Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,362,154	C→T	intergenic (+223024/+31157)	unknown → / ← unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,362,154	0	C	T	100.0%	66.0 / NA	24	intergenic (+223024/+31157)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/11);  total (13/11)

TGAATGCCGGCGATAATTTTCATCAGCGTGGATTTCCCCGCCCCGTTACCGCCGAGCAGTGCGTGCACTTCACCGGCATATAAGGAAAAATCAATGGCTTTCAGCACTTCCACGCCGGAAAACGACTTACCGATGTTTCGGGCGGATATTAAGCATTTTGGCTCTGAAATGTGAGTTTGCATCTTTTACCTGCCGCATAGAATGAACAAATGTATTTAAAAAAAACATTTTTTCAAAATACTAGTCAGGAATGCACAAAATTTCTGAGAACTAGATCACAAATCTGGATAAGATTATCTAAAAATCGCCTTTAATTTTTAAAAAAGTGATCTCCATCATGCTTTTACAAAAAAACGGTAAGAACAATTGATAATAATAAAAAACAAATGAATAATACCCAAAATCAATAAAAACAAATAAATCATATGAACGAATTAATGACGGATAATC  >  1/1361921‑1362370                                                                                                                                                                                                                                          |                                                                                                                                                                                                                         tGAATGCCGGCGATAATTTTCATCAGCGTGGATTTCCCCGCCCCGTTACCGCCGAGCAGTGCGTGCACTTCACCGGCATATAAGGAAAAATCAATGGCTTTCAGCACTTCCACGCCGGAAAACGACTTACCGATGTTTCGAGCGGATATTAAGCATTTTGGCTCTGAAATGTGAGTTTGCATCTTTTACCTGCCGCATAGAATGAACAAATGTATTTAAAAAAAACATTTTTTTAAAATACTAGTCAGGaa                                                                                                                                                                                                         <  1:741126/251‑1 (MQ=255)  gAATGCCGGCGATAATTTTCATCAGCGTGGATTTCCCCGCCCCGTTACCGCCGAGCAGTGCGTGCACTTCACCGGCATATAAGGAAAAATCAATGGCTTTCAGCACTTCCACGCCGGAAAACGACTTACCGATGTTTCGAGCGGATATTAAGCATTTTGGCTCTGAAATGTGAGTTTGCATCTTTTACCTGCCGCATAGAATGAACAAATGTATTTAAAAAAAACATTTTTTTAAAATACTAGTCAGGaa                                                                                                                                                                                                         >  1:880307/1‑250 (MQ=255)               aaTTTTCATCAGCGTGGATTTCCCCGCCCCGTTACCGCCGAGCAGTGCGTGCACTTCACCGGCATATAAGGAAAAATCAATGGCTTTCAGCACTTCCACGCCGGAAAACGACTTACCGATGTTTCGAGCGGATATTAAGCATTTTGGCTCTGAAATGTGAGTTTGCATCTTTTACCTGCCGCATAGAATGAACAAATGTATTTAAAAAAAACATTTTTTTAAAATACTAGTc                                                                                                                                                                                                              <  1:312919/232‑1 (MQ=255)                                aTTTCCCCGCCCCGTTACCGCCGAGCAGTGCGTGCACTTCACCGGCATATAAGGAAAAATCAATGGCTTTCAGCACTTCCACGCCGGAAAACGACTTACCGATGTTTCGAGCGGATATTAAGCATTTTGGCTCTGAAATGTGAGTTTGCATCTTTTACCTGCCGCATAGAATGAACAAATGTATTTAAAAAAAACATTTTTTTAAAATACTAGTCAGGAATGCACAAAATTTCTGAGAACTAGATCACaa                                                                                                                                                                           <  1:659379/250‑1 (MQ=255)                                  ttCCCCGCCCCGTTACCGCCGAGCAGTGCGTGCACTTCACCGGCATATAAGGAAAAATCAATGGCTTTCAGCACTTCCACGCCGGAAAACGACTTACCGATGTTTCGAGCGGATATTAAGCATTTTGGCTCTGAAATGTGAGTTTGCATCTTTTACCTGCCGCATAGAATGAACAAATGTATTTAAAAAAAACATTTTTTTAAAATACTAGTCAGGAATGCACAAAATTTCTGAGAACTAGATCACAAATc                                                                                                                                                                        <  1:770588/251‑1 (MQ=255)                                       cgccccgTTACCGCCGAGCAGTGCGTGCACTTCACCGGCATATAAGGAAAAATCAATGGCTTTCAGCACTTCCACGCCGGAAAACGACTTACCGATGTTTCGAGCGGATATTAAGCATTTTGGCTCTGAAATGTGAGTTTGCATCTTTTACCTGCCGCATAGAATGAACAAATGTATTTAAAAAAAACATTTTTTTAAAATACTAGTCAGGAATGCACAAAATTTCTGAGAACTAGATCACAAATCTGGAt                                                                                                                                                                   <  1:486868/251‑1 (MQ=255)                                         ccccgTTACCGCCGAGCAGTGCGTGCACTTCACCGGCATATAAGGAAAAATCAATGGCTTTCAGCACTTCCACGCCGGAAAACGACTTACCGATGTTTCGAGCGGATATTAAGCATTTTGGCTCTGAAATGTGAGTTTGCATCTTTTACCTGCCGCATAGAATGAACAAATGTATTTAAAAAAAACATTTTTTTAAAATACTAGTc                                                                                                                                                                                                              <  1:822660/206‑1 (MQ=255)                                                 ccgccgAGCAGTGCGTGCACTTCACCGGCATATAAGGAAAAATCAATGGCTTTCAGCACTTCCACGCCGGAAAACGACTTACCGATGTTTCGAGCGGATATTAAGCATTTTGGCTCTGAAATGTGAGTTTGCATCTTTTACCTGCCGCATAGAATGAACAAATGTATTTAAAAAAAACATTTTTTTAAAATACTAGTCAGGAATGCACAAAATTTCTGAGAACTAGATCACAAATCTGGATAAGAt                                                                                                                                                              <  1:348258/246‑1 (MQ=255)                                                           gtgcgtgcACTTCACCGGCATATAAGGAAAAATCAATGGCTTTCAGCACTTCCACGCCGGAAAACGACTTACCGATGTTTCGAGCGGATATTAAGCATTTTGGCTCTGAAATGTGAGTTTGCATCTTTTACCTGCCGCATAGAATGAACAAATGTATTTAAAAAAAACATTTTTTTAAAATACTAGTCAGGAATGCACAAAATTTCTGAGAACTAGATCACAAATCTGGATAAGATTATCTAAAAATCGc                                                                                                                                                >  1:837841/1‑250 (MQ=255)                                                            tgcgtgcACTTCACCGGCATATAAGGAAAAATCAATGGCTTTCAGCACTTCCACGCCGGAAAACGACTTACCGATGTTTCGAGCGGATATTAAGCATTTTGGCTCTGAAATGTGAGTTTGCATCTTTTACCTGCCGCATAGAATGAACAAATGTATTTAAAAAAAACATTTTTTTAAAATACTAGTCAgg                                                                                                                                                                                                           >  1:151273/1‑190 (MQ=255)                                                                  cACTTCACCGGCATATAAGGAAAAATCAATGGCTTTCAGCACTTCCACGCCGGAAAACGACTTACCGATGTTTCGAGCGGATATTAAGCATTTTGGCTCTGAAATGTGAGTTTGCATCTTTTACCTGCCGCATAGAATGAACAAATGTATTTAAAAAAAACATTTTTTTAAAATACTAGTCAGGAATGCACAAAATTTCTGAGAACTAGATCACAAATCTGGATAAGATTATCTAAAAATCGCCTTTAAtt                                                                                                                                        <  1:328985/251‑1 (MQ=255)                                                                            gCATATAAGGAAAAATCAATGGCTTTCAGCACTTCCACGCCGGAAAACGACTTACCGATGTTTCGAGCGGATATTAAGCATTTTGGCTCTGAAATGTGAGTTTGCATCTTTTACCTGCCGCATAGAATGAACAAATGTATTTAAAAAAAACATTTTTTTAAAATACTAGTCAGGAATGCACAAAATTTCTGAGAACTAGATCACAAATCTGGATAAGATTATCTaaaaa                                                                                                                                                    >  1:391742/1‑229 (MQ=255)                                                                                                ggCTTTCAGCACTTCCACGCCGGAAAACGACTTACCGATGTTTCGAGCGGATATTAAGCATTTTGGCTCTGAAATGTGAGTTTGCATCTTTTACCTGCCGCATAGAATGAACAAATGTATTTAAAAAAAACATTTTTTTAAAATACTAGTCAGGAATGCACAAAATTTCTGAGAACTAGATCACAAATCTGGATAAGAt                                                                                                                                                              >  1:208877/1‑199 (MQ=255)                                                                                                                          aCGACTTACCGATGTTTCGAGCGGATATTAAGCATTTTGGCTCTGAAATGTGAGTTTGCATCTTTTACCTGCCGCATAGAATGAACAAATGTATTTAAAAAAAACATTTTTTTAAAATACTAGTCAGGAATGCACAAAATTTCTGAGAACTAGATCACAAATCTGGATAAGATTATCTAAAAATCGCCTTTAATTTTTAAAAAAGTGATCTCCATCATGCTTTTACAAAAAAACGGTAAGAACAATTGata                                                                                <  1:840746/251‑1 (MQ=255)                                                                                                                                           cgagCGGATATTAAGCATTTTGGCTCTGAAATGTGAGTTTGCATCTTTTACCTGCCGCATAGAATGAACAAATGTATTTAAAAAAAACATTTTTTTAAAATACTAGTCAGGAATGCACAAAATTTCTGAGAACTAGATCACAAATCTGGATAAGATTATCTAAAAATCGCCTTTAATTTTTAAAAAAGTGATCTCCATCATGCTTTTACAAAAAAACGGTAAGAACAATTGATAATAATAAAAAACAAATg                                                               <  1:296625/248‑1 (MQ=255)                                                                                                                                                 gATATTAAGCATTTTGGCTCTGAAATGTGAGTTTGCATCTTTTACCTGCCGCATAGAATGAACAAATGTATTTAAAAAAAACATTTTTTTAAAATACTAGTCAGGAATGCACAAAATTTCTGAGAACTAGATCACAAATCTGGATAAGATTATCTAAAAATCGCCTTTAGTTTTTAAAAAAGTGATCTCCATCATGCTTTTACAAAAAAACGGTAAGAACAATTg                                                                                   >  1:454612/1‑225 (MQ=255)                                                                                                                                                  atatTAAGCATTTTGGCTCTGAAATGTGAGTTTGCATCTTTTACCTGCCGCATAGAATGAACAAATGTATTTAAAAAAAACATTTTTTTAAAATACTAGTCAGGAATGCACAAAATTTCTGAGAACTAGATCACAAATCTGGATAAGAt                                                                                                                                                              >  1:680055/1‑149 (MQ=255)                                                                                                                                                  atatTAAGCATTTTGGCTCTGAAATGTGAGTTTGCATCTTTTACCTGCCGCATAGAATGAACAAATGTATTTAAAAAAAACATTTTTTTAAAATACTAGTCAGGAATGCACAAAATTTCTGAGAACTAGATCACAAATCTGGATAAGATTATCTAAAAATCGCCTTTAATTTTTAAAAAAGTGATCTCCATCATGCTTTTACAAAAAAACGGTAAGAACAATTg                                                                                   >  1:743085/1‑224 (MQ=255)                                                                                                                                                                               gTTTGCATCTTTTACCTGCCGCATAGAATGAACAAATGTATTTAAAAAAAACATTTTTTTAAAATACTAGTCAGGAATGCACAAAATTTCTGAGAACTAGATCACAAATCTGGATAAGATTATCTAAAAATCGCCTTTAATTTTTAAAAAAGTGATCTCCATCATGCTTTTACAAAAAAACGGTAAGAACAATTGATAATAATAAAAAACAAATGAATAATACCCAAAATCAATAAAAACAAATAAATCat                           >  1:724181/1‑251 (MQ=255)                                                                                                                                                                                      tCTTTTACCTGCCGCATAGAATGAACAAATGTATTTAAAAAAAACATTTTTTTAAAATACTAGTCAGGAATGCACAAAATTTCTGAGAACTAGATCACAAATCTGGATAAGATTATCTAAAAATCGCCTTTAATTTTTAAAAAAGTGATCTCCATCATGCTTTTACAAAAAAACGGTAAGAACAATTGATAATAATAAAAAACAAATGAATAATACCCAAAATCAATAAAAACAAATAAATCATATGAACg                    >  1:809428/1‑251 (MQ=255)                                                                                                                                                                                                gCCGCATAGAATGAACAAATGTATTTAAAAAAAACATTTTTTTAAAATACTAGTCAGGAATGCACAAAATTTCTGAGAACTAGATCACAAATCTGGATAAGATTATCTAAAAATCGCCTTTAATTTTTAAAAAAGTGATCTCCATCATGCTTTTACAAAAAAACGGTAAGAACAATTGATAATAATAAAAAACAAATGAATAATACCCAAAATCAATAAAAACAAATAAATCATATGAACGAATTAATGa           >  1:645428/1‑250 (MQ=255)                                                                                                                                                                                                        gAATGAACAAATGTATTTAAAAAAAACATTTTTTTAAAATACTAGTCAGGAATGCACAAAATTTCTGAGAACTAGATCACAAATCTGGATAAGATTATCTAAAAATCGCCTTTAATTTTTAAAAAAGTGATCTCCATCATGCTTTTACAAAAAAACGGTAAGAACAATTGATAATAATAAAAAACAAATGAATAATACCCAAAATCAATAAAAACAAATAAATCa                            <  1:653878/225‑1 (MQ=255)                                                                                                                                                                                                            gAACAAATGTATTTAAAAAAAACATTTTTTTAAAATACTAGTCAGGAATGCACAAAATTTCTGAGAACTAGATCACAAATCTGGATAAGATTATCTaa                                                                                                                                                       >  1:99412/1‑98 (MQ=255)                                                                                                                                                                                                                     tATTTAAAAAAAACATTTTTTTAAAATACTAGTCAGGAATGCACAAAATTTCTGAGAACTAGATCACAAATCTGGATAAGATTATCTAAAAATCGCCTTTAATTTTT‑AAAAAGTGATCTCCATCATGCTTTTACAAAAAAACGGTAAGAACAATTGATAATAATAAAAAACAAATGAATAATACCCAAAATCAATAAAAACAAATAAATCATATGAACGAATTAATGACGGATAATc  >  1:176639/1‑237 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                         TGAATGCCGGCGATAATTTTCATCAGCGTGGATTTCCCCGCCCCGTTACCGCCGAGCAGTGCGTGCACTTCACCGGCATATAAGGAAAAATCAATGGCTTTCAGCACTTCCACGCCGGAAAACGACTTACCGATGTTTCGGGCGGATATTAAGCATTTTGGCTCTGAAATGTGAGTTTGCATCTTTTACCTGCCGCATAGAATGAACAAATGTATTTAAAAAAAACATTTTTTCAAAATACTAGTCAGGAATGCACAAAATTTCTGAGAACTAGATCACAAATCTGGATAAGATTATCTAAAAATCGCCTTTAATTTTTAAAAAAGTGATCTCCATCATGCTTTTACAAAAAAACGGTAAGAACAATTGATAATAATAAAAAACAAATGAATAATACCCAAAATCAATAAAAACAAATAAATCATATGAACGAATTAATGACGGATAATC  >  1/1361921‑1362370

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑