Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,361,427	C→T	intergenic (+222297/+31884)	unknown → / ← unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,361,427	0	C	T	100.0%	165.5 / NA	55	intergenic (+222297/+31884)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/39);  total (16/39)

ATTCGGTTCGCCCTGCGCCAACCACGCCCGCCAAGCCTAAAATTTCACCGGGATAAATATCAAAAGTGACATTTTTAAATCCTTCGCCCGTCAAATCCTGCACCTGCAAAATCGGCTTATCGGTAGCTTTCACACGGTTATGTCCCGGCAACGCCAGCCATAATTTTTGGCTTTCCGTTAATTGGGCATTTTTCGCCTTCGGCGTGATGGCGGTAATCACATCGTCTTTATTCAACTCGGCAGTACTGCCGGTTAGTGCAATATAGCCGTCGCGCATGACGCTAATACGATGAGAAATCTGCCACACTTCGGGCAGTTTGTGAGAAATGAAGACAATGCCCACACCCTTTTCCAATAACGAACGAATTTTATTAAACAGGTTCTCCGTTTCAATTGGCGTTAAAGAGGCGGTGGGTTCGTCCAGAATCAGAATAGACGCATTACGCATAAGGCCACGCA  >  1/1361192‑1361650                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                aTTCGTTTCGCCCTGCGCCAACCACGCCCGCCAAGCCTAAAATTTCACCGGGATAAATATCAAAAGTGACATTTTTAAATCCTTCGCCCGTCAAATCCTGCACCTGCAAAATCGGCTTATCGGTAGCTTTCACACGGTTATGTCCCGGCAACGCCAGCCATAATTTTTGGCTTTCCGTTAATTGGGCATTTTTCGCCTTCGGCGTGATGGCGGTAATCACATCGTCTTTATTCAATTCGGCAGTACTGCCg                                                                                                                                                                                                                  <  1:351483/251‑1 (MQ=255)    cGGTTCGCCCTGCGCCAACCACGCCCGCCAAGCCTAAAATTTCACCGGGATAAATATCAAAAGTGACATTTTTAAATCCTTCGCCCGTCAGATCCTGCACCTGCAAAATCGGCTTATCGGTAGCTTTCACACGGTTATGTCCCGGCAACGCCAGCCATAATTTTTGGCTTTCCGTTAATTGGGCATTTTTCGCCTTCGGCGTGATGGCGGTAATCACATCGTCTTTATTCAATTCGGCAGTACTACCGGtt                                                                                                                                                                                                               <  1:557283/251‑1 (MQ=255)          gCCCTGCGCCAACCACGCCCGCCAAGCCTAAAATTTCACCGGGATAAATATCAAAAGTGACATTTTTAAATCCTTCGCCCGTCAAATCCTGCACCTGCAAAATCGGCTTATCGGTAGCTTTCACACGGTTATGTCCCGGCAACGCCAGCCATAATTTTTGGCTTTCCGTTAATTGGGCATTTTTCGCCTTCGGCGTGATGGCGGTAATCACATCGTCTTTATTCAATTCGGCAGTACTGCCGGTTAGTGCa                                                                                                                                                                                                         >  1:296601/1‑251 (MQ=255)              tGCGCCACCCACGCCCGCCAAGCCTAAAATTTCACCGGGATAAATATCAAAAGTGACATTTTTAAATCCTTCGCCCGTCAAATCCTGCACCTGCAAAATCGGCTTATCGGTAGCTTTCACACGGTTATGTCCCGGCAACGCCAGCCATAATTTTTGGCTTTCCGTTAATTGGGCATTTTTCGCCTTCGGCGTGATGGCGGTAATCACATCGTCTTTATTCAATTCGGCAGTACTGCCGGTTAGTGCAatat                                                                                                                                                                                                     <  1:692668/251‑1 (MQ=255)              tGCGCCAACCACGCCCGCCAAGCCTAAAATTTCGCCGGGATAAATATCAAAAGTGACATTTTTAAATCCTTCGCCCGTCAAATCCTGCACCTGCAAAATCGGCTTATCGGTAGCTTTCACACGGTTATGTCCCGGCAACGCCAGCCATAATTTTTGGCTTTCCGTTAATTGGGCATTTTTCGCCTTCGGCGTGATGGCGGTAATCACATCGTCTTTATTCAATTCGGCAGTACTGCCGGTTAGTGCAatat                                                                                                                                                                                                     <  1:30451/251‑1 (MQ=255)              tGCGCCAACCACGCCCGCCAAGCCTAAAATTTCACCGGGATAAATATCAAAAGTGACATTTTTAAATCCTTCGCCCGTCAAATCCTGCACCTGCAAAATCGGCTTATCGGTAGCTTTCACACGGTTATGTCCCGGCAACGCCAGCCATAATTTTTGGCTTTCCGTTAATTGGGCATTTTTCGCCTTCGGCGTGATGGCGGTAATCACATCGTCTTTATTCAATTCGGCAGTACTGCCGGTTAGTGCAatat                                                                                                                                                                                                     <  1:398628/251‑1 (MQ=255)               gcgcCAACCACGCCCGCCAAGCCTAAAATTTCACCGGGATAAATATCAAAAGTGACATTTTTAAATCCTTCGCCCGTCAAATCCTGCACCTGCAAAATCGGCTTATCGGTAGCTTTCACACGGTTATGTCCCGGCAACGCCAGCCATAATTTTTGGCTTTCCGTTAATTGGGCATTTTTCGCCTTCGGCGTGATGGCGGTAATCACATCGTCTTTATTCAATTCGGCAGTACTGCCGGTTAGTGCAatat                                                                                                                                                                                                     >  1:758233/1‑250 (MQ=255)                 cccAACCACGCCCGCCAAGCCTAAAATTTCACCGGGATAAATATCAAAAGTGACATTTTTAAATCCTTCGCCCGTCAAATCCTGCACCTGCAAAATCGGCTTATCGGTAGCTTTCACACGGTTATGTCCCGGCAACGCCAGCCATAATTTTTGGCTTTCCGTTAATTGGGCATTTTTCGCCTTCGGCGTGATGGCGGTAATCACATCGTCTTTATTCAATTCGGCAGTACTGCCGGTTAGTGCAATATAGc                                                                                                                                                                                                  <  1:27871/250‑1 (MQ=255)                  ccAACCACGCCCGCCAAGCCTAAAATTTCACCGGGATAAATATCAAAAGTGACATTTTTAAATCCTTCGCCCGTCAAATCCTGCACCTGCAAAATCGGCTTATCGGTAGCTTTCACACGGTTATGTCCCGGCAACGCCAGCCATAATTTTTGGCTTTCCGTTAATTGGGCATTTTTCGCCTTCGGCGTGATGGCGGTAATCACATCGTCTTTATTCAATTCGGCAGTACTGCCGGTTAGTGCAATATAGc                                                                                                                                                                                                  <  1:806485/250‑1 (MQ=255)                   cAACCACGCCCGCCAAGCCTAAAATTTCACCGGGATAAATATCAAAAGTGACATTTTTAAATCCTTCGCCCGTCAAATCCTGCACCTGCAAAATCGGCTTATCGGTAGCTTTCACACGGTTATGTCCCGGCAACGCCAGCCATAATTTTTGGCTTTCCGTTAATTGGGCATTTTTCGCCTTCGGCGTGATGGCGGTAATCACATCGTCTTTATTCAATTCGGCAGTACTGCCGGTTAGTGCAATATAGCCg                                                                                                                                                                                                <  1:318424/251‑1 (MQ=255)                                    ccTAAAATTTCACCGGGATAAATATCAAAAGTGACATTTTTAAATCCTTCGCCCGTCAAATCCTGCACCTGCAAAATCGGCTTATCGGTAGCTTTCACACGGTTATGTCCCGGCAACGCCAGCCATAATTTTTGGCTTTCCGTTAATTGGGCATTTTTCGCCTTCGGCGTGATGGCGGTAATCACATCGTCTTTATTCAATTCGGCAGTACTGCCGGTTAGTGCAATATAGCCGTCGCGCATGACGCTAAt                                                                                                                                                                               >  1:339650/1‑251 (MQ=255)                                     cTAAAATTTCACCGGGATAAATATCAAAAGTGACATTTTTAAATCCTTCGCCCGTCAAATCCTGCACCTGCAAAATCGGCTTATCGGTAGCTTTCACACGGTTATGTCCCGGCAACGCCAGCCATAATTTTTGGCTTTCCGTTAATTGGGCATTTTTCGCCTTCGGCGTGATGGCGGTAATCACATCGTCTTTATTCAATTCGGCAGTACTGCCGGTTAGTGCAATATAGCCGTCGCGCATGACGCTAATa                                                                                                                                                                              <  1:257603/251‑1 (MQ=255)                                     cTAAAATTTCACCGGGATAAATATCAAAAGTGACATTTTTAAATCCTTCGCCCGTCAAATCCTGCACCTGCAAAATCGGCTTATCGGTAGCTTTCACACGGTTATGTCCCGGCAACGCCAGCCATAATTTTTGGCTTTCCGTTAATTGGGCATTTTTCGCCTTCGGCGTGATGGCGGTAATCACATCGTCTTTATTCAATTCGGCAGTACTGCCGGTTAGTGCAATATAGCCGTCGCGCATGACGCTAATa                                                                                                                                                                              <  1:859956/251‑1 (MQ=255)                                      tAAAATTTCACCGGGATAAATATCAAAAGTGACATTTTTAAATCCTTCGCCCGTCAAATCCTGCACCTGCAAAATCGGCTTATCGGTAGCTTTCACACGGTTATGTCCCGGCAACGCCAGCCATAATTTTTGGCTTTCCGTTAATTGGGCATTTTTCGCCTTCGGCGTGATGGCGGTAATCACATCGTCTTTATTCAATTCGGCAGTACTGCCGGTTAGTGCAATATAGCCGTCGCGCATGACGCTAATAc                                                                                                                                                                             <  1:515428/251‑1 (MQ=255)                                      tAAAATTTCACCGGGATAAATATCAAAAGTGACATTTTTAAATCCTTCGCCCGTCAAATCCTGCACCTGCAAAATCGGCTTATCGGTAGCTTTCACACGGTTATGTCCCGGCAACGCCAGCCATAATTTTTGGCTTTCCGTTAATTGGGCATTTTTCGCCTTCGGCGTGATGGCGGTAATCACATCGTCTTTATTCAATTCGGCAGTACTGCCGGTTAGTGCAATATAGCCGTCGCGCATGACGCTAATAc                                                                                                                                                                             <  1:42827/251‑1 (MQ=255)                                      tAAAATTTCACCGGGATAAATATCAAAAGTGACATTTTTAAATCCTTCGCCCGTCAAATCCTGCACCTGCAAAATCGGCTTATCGGTAGCTTTCACACGGTTATGTCCCGGCAACGCCAGCCATAATTTTTGGCTTTCCGTTAATTGGGCATTTTTCGCCTTCGGCGTGATGGCGGTAATCACATCGTCTTTATTCAATTCGGCAGTACTGCCGGTTAGTGCAATATAGCCGTCGCGCATGACGCTAATAc                                                                                                                                                                             <  1:307204/251‑1 (MQ=255)                                                          tatCAAAAGTGACATTTTTAAATCCTTCGCCCGTCAAATCCTGCACCTGCAAAATCGGCTTATCGGTAGCTTTCACACGGTTATGTCCCGGCAACGCCAGCCATAATTTTTGGCTTTCCGTTAATTGGGCATTTTTCGCCTTCGGCGTGATGGCGGTAATCACATCGTCTTTATTCAATTCGGCAGTACTGCCGGTTAGTGCAATATAGCCGTCGCGCATGACGCTAATACGATGAGAAATCTGCcaca                                                                                                                                                           >  1:678054/1‑249 (MQ=255)                                                          tatCAAAAGTGACATTTTTAAATCCTTCGCCCGTCAAATCCTGCACCTGCAAAATCGGCTTATCGGTAGCTTTCACACGGTTATGTCCCGGCAACGCCAGCCATAATTTTTGGCTTTCCGTTAATTGGGCATTTTTCGCCTTCGGCGTGATGGCGGTAATCACATCGTCTTTATTCAATTCGGCAGTACTGCCGGTTAGTGCAATATAGCCGTCGCGCATGACGCTAATACGATGAGAAATCTGCcaca                                                                                                                                                           >  1:16840/1‑249 (MQ=255)                                                                aaGTGACATTTTTAAATCCTTCGCCCGTCAAATCCTGCACCTGCAAAATCGGCTTATCGGTAGCTTTCACACGGTTATGTCCCGGCAACGCCAGCCATAATTTTTGGCTTTCCGTTAATTGGGCATTTTTCGCCTTCGGCGTGATGGCGGTAATCACATCGTCTTTATTCAATTCGGCAGTACTGCCGGTTAGTGCAATATAGCCGTCGCGCATGACGCTAATACGATGAGAAATCTGCCACACTTCGGGc                                                                                                                                                   <  1:827493/251‑1 (MQ=255)                                                                   tGACATTTTTAAATCCTTCGCCCGTCAAATCCTGCACCTGCAAAATCGGCTTATCGGTAGCTTTCACACGGTTATGTCCCGGCAACGCCAGCCATAATTTTTGGCTTTCCGTTAATTGGGCATTTTTCGCCTTCGGCGTGATGGCGGTAATCACATCGTCTTTATTCAATTCGGCAGTACTGCCGGTTAGTGCAATATAGCCGTCGCGCATGACGCTAATACGATGAGAAATCTGCCACACTTCGGGCAg                                                                                                                                                 <  1:51260/250‑1 (MQ=255)                                                                                               aTCCTGCACCTGCAAAATCGGCTTATCGGTAGCTTTCACACGGTTATGTCCCGGCAACGCCAGCCATAATTTTTGGCTTTCCGTTAATTGGGCATTTTTCGCCTTCGGCGTGATGGCGGTAATCACATCGTCTTTATTCAATTCGGCAGTACTGCCGGTTAGTGCAATATAGCCGTCGCGCATGACGCTAATACGATGAGAAATCTGCcac                                                                                                                                                            <  1:730779/211‑1 (MQ=255)                                                                                               aTCCTGCACCTGCAAAATCGGCTTATCGGTAGCTTTCACACGGTTATGTCCCGGCAACGCCAGCCATAATTTTTGGCTTTCCGTTAATTGGGCATTTTTCGCCTTCGGCGTGATGGCGGTAATCACATCGTCTTTATTCAATTCGGCAGTACTGCCGGTTAGTGCAATATAGCCGTCGCGCATGACGCTAATACGATGAGAAATCTGCCACACTTCGGGCAGTTTGTGAGAAATGAAGACAATGCCcaca                                                                                                                     >  1:764617/1‑250 (MQ=255)                                                                                                tCCTGCACCTGCAAAATCGGCTTATCGGTAGCTTTCACACGGTTATGTCCCGGCAACGCCAGCCATAATTTTTGGCTTTCCGTTAATTGGGCATTTTTCGCCTTCGGCGTGATGGCGGTAATCACATCGTCTTTATTCAATTCGGCAGTACTGCCGGTTAGTGCAATATAGCCGTCGCGCATGACGCTAATACGATGAGAAATCTGCCACACTTCGGGCAGTTTGTGAGAAATGAAGACAATGCCCACAcc                                                                                                                   <  1:473668/251‑1 (MQ=255)                                                                                                        ctgcaAAATCGGCTTATCGGTAGCTTTCACACGGTTATGTCCCGGCAACGCCAGCCATAATTTTTGGCTTTCCGTTAATTGGGCATTTTTCGCCTTCGGCGTGATGGCGGTAATCACATCGTCTTTATTCAATTCGGCAGTACTGCCGGTTAGTGCAATATAGCCGTCGCGCATGACGCTAATACGATGAGAAATCTGCCACACTTCGGGCAGTTTGTGAGAAATGAAGACAATGCCC‑CACCCTTTTCCaa                                                                                                          <  1:856936/251‑1 (MQ=255)                                                                                                         tgcaAAATCGGCTTATCGGTAGCTTTCACACGGTTATGTCCCGGCAACGCCAGCCATAATTTTTGGCTTTCCGTTAATTGGGCATTTTTCGCCTTCGGCGTGATGGCGGTAATCACATCGTCTTTATTCAATTCGGCAGTACTGCCGGTTAGTGCAATATAGCCGTCGCGCATGACGCTAATACGATGAGAAATCTGCCACACTTCGGGCAGTTTGTGAGAAATGAAGAc                                                                                                                               <  1:96041/230‑1 (MQ=255)                                                                                                           caAAATCGGCTTATCGGTAGCTTTCACACGGTTATGTCCCGGCAACGCCAGCCATAATTTTTGGCTTTCCGTTAATTGGGCATTTTTCGCCTTCGGCGTGATGGCGGTAATCACATCGTCTTTATTCAATTCGGCAGTACTGCCGGTTAGTGCAATATAGCCGTCGCGCATGACGCTAATACGATGAGAAATCTGCCACACTTCGGGCAGTTTGTGAGAAATGAAGAc                                                                                                                               <  1:184935/228‑1 (MQ=255)                                                                                                                                     cacGGTTATGTCCCGGCAACGCCAGCCATAATTTTTGGCTTTCCGTTAATTGGGCATTTTTCGCCTTCGGCGTGATGGCGGTAATCACATCGTCTTTATTCAATTCGGCAGTACTGCCGGTTAGTGCAATATAGCCGTCGCGCATGACGCTAATACGATGAGAAATCTGCCACACTTCGGGCAGTTTGTGAGAAATGAAGACAATGCCCACACCCTTTTCCAATAACGAACGAATTTTATTAAACAGGTtc                                                                              <  1:74658/251‑1 (MQ=255)                                                                                                                                       cGGTTATGTCCCGGCAACGCCAGCCATAATTTTTGGCTTTCCGTTAATTGGGCATTTTTCGCCTTCGGCGTGATGGCGGTAATCACATCGTCTTTATTCAATTCGGCAGTACTGCCGGTTAGTGCAATATAGCCg                                                                                                                                                                                                >  1:50384/1‑135 (MQ=255)                                                                                                                                       cGGTTATGTCCCGGCAACGCCAGCCATAATTTTTGGCTTTCCGTTAATTGGGCATTTTTCGCCTTCGGCGTGATGGCGGTAATCACATCGTCTTTATTCAATTCGGCAGTACTGCCGGTTAGTGCAATATAGCCGTCGCGCATGACGCTAATACGATGAGAAATCTGCCACACTTCGGGCAGTTTGTGAGAAATGAAGACAATGCCCACACCCTTTTCCAATAACGAACGAATTTTATTAAACAGGTtctc                                                                            <  1:801930/251‑1 (MQ=255)                                                                                                                                         gTTATGTCCCGGCAACGCCAGCCATAATTTTTGGCTTTCCGTTAATTGGGCATTTTTCGCCTTCGGCGTGATGGCGGTAATCACATCGTCTTTATTCAATTCGGCAGTACTGCCGGTTAGTGCAATATAGCCGTCGCGCATGACGCTAATACGATGAGAAATCTGCCacac                                                                                                                                                          <  1:699472/171‑1 (MQ=255)                                                                                                                                         gTTATGTCCCGGCAACGCCAGCCATAATTTTTGGCTTTCCGTTAATTGGGCATTTTTCGCCTTCGGCGTGATGGCGGTAATCACATCGTCTTTATTCAATTCGGCAGTACTGCCGGTTAGTGCAATATAGCCGTCGCGCATGACGCTAATACGATGAGAAATCTGCCacac                                                                                                                                                          <  1:825990/171‑1 (MQ=255)                                                                                                                                         gTTATGTCCCGGCAACGCCAGCCATAATTTTTGGCTTTCCGTTAATTGGGCATTTTTCGCCTTCGGCGTGATGGCGGTAATCACATCGTCTTTATTCAATTCGGCAGTACTGCCGGTTAGTGCAATATAGCCGTCGCGCATGACGCTAATACGATGAGAAATCTGCCACACTTCGGGCAGTTTGTGAGAAATGAAGACAATGCCCACACCCTTTTCCAATaa                                                                                                       >  1:173384/1‑222 (MQ=255)                                                                                                                                         gTTATGTCCCGGCAACGCCAGCCATAATTTTTGGCTTTCCGTTAATTGGGCATTTTTCGCCTTCGGCGTGATGGCGGTAATCACATCGTCTTTATTCAATTCGGCAGTACTGCCGGTTAGTGCAATATAGCCGTCGCGCATGACGCTAATACGATGAGAAATCTGCCACACTTCGGGCAGTTTGTGAGAAATGAAGACAATGCCCACACCCTTTTCCAATAACGAACGAATTTTATTAAAc                                                                                    >  1:852943/1‑241 (MQ=255)                                                                                                                                         gTTATGTCCCGGCAACGCCAGCCATAATTTTTGGCTTTCCGTTAATTGGGCATTTTTCGCCTTCGGCGTGATGGCGGTAATCACATCGTCTTTATTCAATTCGGCAGTACTGCCGGTTAGTGCAATATAGCCGTCGCGCATGACGCTAATACGATGAGAAATCTGCCACACTTCGGGCAGTTTGTGAGAAATGAAGACAATGCCCACACCCTTTTCCAATAACGAACGAATTTTATTAAACAGGTTCTCCg                                                                          >  1:365481/1‑251 (MQ=255)                                                                                                                                         gTTATGTCCCGGCAACGCCAGCCATAATTTTTGGCTTTCCGTTAATTGGGCATTTTTCGCCTTCGGCGTGATGGCGGTAATCACATCGTCTTTATTCAATTCGGCAGTACTGCCGGTTAGTGCAATATAGCCGTCGCGCATGACGCTAATACGATGAGAAATCTGCCACACTTCGGGCAGTTTGTGAGAAATGAAGACAATGCCCACACCCTTTTCCAATAACGAACGAATTTTATTAAACAGGTTCTCCg                                                                          >  1:200351/1‑251 (MQ=255)                                                                                                                                         gTTATGTCCCGGCAACGCCAGCCATAATTTTTGGCTTTCCGTTAATTGGGCATTTTTCGCCTTCGGCGTGATGGCGGTAATCACATCGTCTTTATTCAATTCGGCAGTACTGCCGGTTAGTGCAATATAGCCGTCGCGCATGACGCTAATACGATGAGAAATCTGCCACACTTCGGGAAGTTTGTGAGAAATGAAGACAATGCCCACACCCTTTTCCAATAACGAAAGAATTTTATTAAACAGGTTCTCCg                                                                          >  1:338321/1‑251 (MQ=255)                                                                                                                                             tGTCCCGGCAACGCCAGCCATAATTTTTGGCTTTCCGTTAATTGGGCATTTTTCGCCTTCGGCGTGATGGCGGTAATCACATCGTCTTTATTCAATTCGGCAGTACTGCCGGTTAGTGCAATATAGCCGTCGCGCATGACGCTAATACGATGAGAAATCTGCCACACTTCGGGCAGTTTGTGAGAAATGAAGAc                                                                                                                               >  1:154/1‑194 (MQ=255)                                                                                                                                              gTCCCGGCAACGCCAGCCATAATTTTTGGCTTTCCGTTAATTGGGCATTTTTCGCCTTCGGCGTGATGGCGGTAATCACATCGTCTTTATTCAATTCGGCAGTACTGCCGGTTAGTGCAATATAGCCGTCGCGCATGACGCTAATACGATGAGAAATCTGCCACACTTCGGGCAGTTTGTGAGAAATGAAGACAATGCCCACAcc                                                                                                                   <  1:279078/205‑1 (MQ=255)                                                                                                                                                 ccGGCAACGCCAGCCATAATTTTTGGCTTTCCGTTAATTGGGCATTTTTCGCCTTCGGCGTGATGGCGGTAATCACATCGTCTTTATTCAATTCGGCAGTACTGCCGGTTAGTGCAATATAGCCGTCGCGCATGACGCTAATACGATGAGAAATCTGCCACACTTCGGGCAGTTTGTGAGAAATGAAGACAATGCCCACACCCTTTTCCAATAACGAACGAATTTTATTAAACAGGTTCTCCGTTTCAAtt                                                                  <  1:284651/251‑1 (MQ=255)                                                                                                                                                                aTAATTTTTGGCTTTCCGTTAATTGGGCATTTTTCGCCTTCGGCGTGATGGCGGTAATCACATCGTCTTTATTCAATTCGGCAGTACTGCCGGTTAGTGCAATATAGCCGTCGCGCATGACGCTAATACGATGAGAAATCTGCCACACTTCGGGCAGTTTGTgag                                                                                                                                         <  1:862646/165‑1 (MQ=255)                                                                                                                                                                 tAATTTTTGGCTTTCCGTTAATTGGGCATTTTTCGCCTTCGGCGTGATGGCGGTAATCACATCGTCTTTATTCAATTCGGCAGTACTGCCGGTTAGTGCAATATAGCCGTCGCGCATGACGCTAATACGATGAGAAATCTGCCACACTTCGGGCAGTTTGTGAGAAATGAAGACAATGCCCACACCCTTTTCCAATAACGAACGAATTTTATTAAACAGGTTCTCCGTTTCAATTGGCGTTAAAGAGGCgg                                                  <  1:370564/251‑1 (MQ=255)                                                                                                                                                                   aTTTTTGGCTTTCCGTTAATTGGGCATTTTTCGCCTTCGGCGTGATGGCGGTAATCACATCGTCTTTATTCAATTCGGCAGTACTGCCGGTTAGTGCAATATAGCCGTCGCGCATGACGCTAATACGATGAGAAATCTGCCACACTTCGGGCAGTTTGTGAGAAATGAAGACAATGCCCACACCCTTTTCCAATAACGAACGAATTTTATTAAACAGGTTCTCCGTTTCAATTGGCGTTAAAGAGGCGGTg                                                >  1:235069/1‑251 (MQ=255)                                                                                                                                                                                      ttGGGCATTTTTCGCCTTCGGCGTGATGGCGGTAATCACATCGTCTTTATTCAATTCGGCAGTACTGCCGGTTAGTGCAATATAGCCGTCGCGCATGACGCTAATACGATGAGAAATCTGCCACACTTCGGGCAGTTTGTGAGAAATGAAGACAATGCCCACACCCTTTTCCAATAACGAACGAATTTTATTAAACAGGTTCTCCGTTTCAATTGGCGTTAAAGAGGCGGTGGGTTCGTCcagaatcagaa                             <  1:544965/251‑1 (MQ=255)                                                                                                                                                                                      ttGGGCATTTTTCGCCTTCGGCGTGATGGCGGTAATCACATCGTCTTTATTCAATTCGGCAGTACTGCCGGTTAGTGCAATATAGCCGTCGCGCATGACGCTAATACGATGAGAAATCTGCCACACTTCGGGCAGTTTGTGAGAAATGAAGACAATGCCCACACCCTTTTCCAATAACGAACGAATTTTATTAAACAGGTTCTCCGTTTCAATTGGCGTTAAAGAGGCGGTGGGTTCGTCcagaatcagaa                             <  1:689012/251‑1 (MQ=255)                                                                                                                                                                                         ggCATTTTTCGCCTTCGGCGTGATGGCGGTAATCACATCGTCTTTATTCAATTCGGCAGTACTGCCGGTTAGTGCAATATAGCCGTCGCGCATGACGCTAATACGATGAGAAATCTGCCACACTTCGGGCAGTTTGTGAGAAATGAAGACAATGCCCACACCCTTTTCCAATAACGAACGAATTTTATTAAACAGGTTCTCCGTTTCAATTGGCGTTAAAGAGGCGGTGGGTTCGTCCAGAATCAGAATa                           <  1:793703/250‑1 (MQ=255)                                                                                                                                                                                                          gCGTGATGGCGGTAATCACATCGTCTTTATTCAATTCGGCAGTACTGCCGGTTAGTGCAATATAGCCGTCGCGCATGACGCTAATACGATGAGAAATCTGCCACACTTCGGGCAGTTTGTGAGAAATGAAGACAATGCCCACACCCTTTTCCAATAACGAACGAATTTTATTAAACAGGTTCTCCGTTTCAATTGGCGTTAAAGAGGCGGTGGGTTCGTCCAGAATCAGAATAGACGCATTACGCATAAg          <  1:164924/250‑1 (MQ=255)                                                                                                                                                                                                                tGGCGGTAATCACATCGTCTTTATTCAATTCGGCAGTACTGCCGGTTAGTGCAATATAGCCGTCGCGCATGACGCTAATACGATGAGAAATCTGCCACACTTCGGGCAGTTTGTGAGAAATGAAGACAATGCCCACACCCTTTTCCAATAACGAACGAATTTTATTAAACAGGTTCTCCGTTTCAATTGGCGTTAAAGAGGCGGTGGGTTCGTCCAGAATCAGAATAGACGCATTACGCATAAGGc        <  1:369729/246‑1 (MQ=255)                                                                                                                                                                                                                 ggCGGTAATCACATCGTCTTTATTCAATTCGGCAGTACTGCCGGTTAGTGCAATATAGCCGTCGCGCATGACGCTAATACGATGAGAAATCTGCCACACTTCGGGCAGTTTGTGAGAAATGAAGACAATGCCCACACCCTTTTCCAATAACGAACGAATTTTATTAAACAGGTTCTCCGTTTCAATTGGCGTTAAAGAGGCGGTGGGTTCGTCcagaatc                                 >  1:290288/1‑220 (MQ=255)                                                                                                                                                                                                                  gCGGTAATCACATCGTCTTTATTCAATTCGGCAGTACTGCCGGTTAGTGCAATATAGCCGTCGCGCATGACGCTAATACGATGAGAAATCTGCCACACTTCGGGCAGTTTGTGAGAAATGAAGACAATGCCCACACCCTTTTcc                                                                                                            <  1:293410/144‑1 (MQ=255)                                                                                                                                                                                                                  gCGGTAATCACATCGTCTTTATTCAATTCGGCAGTACTGCCGGTTAGTGCAATATAGCCGTCGCGCATGACGCTAATACGATGAGAAATCTGCCACACTTCGGGCAGTTTGTGAGAAATGAAGACAATGCCCACACCCTTTTCCAATAACGAACGAATTTTATTAAACAGGTTCTCCGTTTCAATTGGCGTTAAAGAGGCGGTGGGGTCGTCCAGAATCAGAATAGACGCATTACGCATAAGGCCACGCa  >  1:401854/1‑250 (MQ=255)                                                                                                                                                                                                                    ggTAATCACATCGTCTTTATTCAATTCGGCAGTACTGCCGGTTAGTGCAATATAGCCGTCGCGCATGACGCTAATACGATGAGAAATCTGCCACACTTCTGGCAGTTTGTGAGAAATGAAGACAATGCCCACACCCTTTTCCAATAACGAACGAATTTTATTAAACAGGTTCTCCGTTTCAAtt                                                                  <  1:314729/184‑1 (MQ=255)                                                                                                                                                                                                                        aTCACATCGTCTTTATTCAATTCGGCAGTACTGCCGGTTAGTGCAATATAGCCGTCGCGCATGACGCTAATACGATGAGAAATCTGCCACACTTCGGGCAGTTTGTGAGAAATGAAGACAATGCCCACACCCTTTTCCAATAACGAACGAATTTTATTAAACAGGTTCTCCGTTTCAATTGGCGTTAAAGAGGCGGTGGGTTCGTCCAGAATCAGAATAGACg                       <  1:295785/223‑1 (MQ=255)                                                                                                                                                                                                                                gTCTTTATTCAATTCGGCAGTACTGCCGGTTAGTGCAATATAGCCGTCGCGCATGACGCTAATACGATGAGAAATCTGCCACACTTCGGGCAGTTTGTGAGAAATGAAGACAATGCCCACACCCTTTTCCAATAACGAACGAATTTTATTAAACAGGTTCTCCGTTTCAATTGGCGTTAAAGAGGCGGTGGGTTCGTCCAGAATCAGAATAGACGCATTACGCa              <  1:377829/224‑1 (MQ=255)                                                                                                                                                                                                                                 tCTTTATTCAATTCGGCAGTACTGCCGGTTAGTGCAATATAGCCGTCGCGCATGACGCTAATACGATGAGAAATCTGCCACACTTCGGGCAGTTTGTGAGAAATGAAGACAATGCCCACACCCTTTTCCAATAACGAACGAATTTTATTAAACAGGTTCTCCGTTTCAATTGGCGTTAAAGAGGCGGTGGGTTCGTCcagaatcagaa                             <  1:527813/208‑1 (MQ=255)                                                                                                                                                                                                                                 tCTTTATTCAATTCGGCAGTACTGCCGGTTAGTGCAATATAGCCGTCGCGCATGACGCTAATACGATGAGAAATCTGCCACACTTCGGGCAGTTTGTGAGAAATGAAGACAATGCCCACACCCTTTTCCAATAACGAACGAATTTTATTAAACAGGTTCTCCGTTTCAATTGGCGTTAAAGAGGCGGTGGGTTCGTCCAGAATCAGAATAGACGCATTACGCATAAGGc        <  1:614404/229‑1 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                ATTCGGTTCGCCCTGCGCCAACCACGCCCGCCAAGCCTAAAATTTCACCGGGATAAATATCAAAAGTGACATTTTTAAATCCTTCGCCCGTCAAATCCTGCACCTGCAAAATCGGCTTATCGGTAGCTTTCACACGGTTATGTCCCGGCAACGCCAGCCATAATTTTTGGCTTTCCGTTAATTGGGCATTTTTCGCCTTCGGCGTGATGGCGGTAATCACATCGTCTTTATTCAACTCGGCAGTACTGCCGGTTAGTGCAATATAGCCGTCGCGCATGACGCTAATACGATGAGAAATCTGCCACACTTCGGGCAGTTTGTGAGAAATGAAGACAATGCCCACACCCTTTTCCAATAACGAACGAATTTTATTAAACAGGTTCTCCGTTTCAATTGGCGTTAAAGAGGCGGTGGGTTCGTCCAGAATCAGAATAGACGCATTACGCATAAGGCCACGCA  >  1/1361192‑1361650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑