Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	9,086	G→A	intergenic (+4479/+121419)	unknown → / ← unknown	tRNA‑Val‑TAC/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	9,086	0	G	A	100.0%	98.6 / NA	36	intergenic (+4479/+121419)	unknown/unknown	tRNA‑Val‑TAC/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/16);  total (20/16)

TAAACAATTTGAGCCCTGTGCAGTGCAGAGCTCAATATTTAACTTAGGTTTAGCTATCCGCCCGGTTTTAGGGTGCAGATCAAAGTGCGATCATTTTCTTTTCTCATTTCTTTTTTCTTGATTAAATCAATTCTAACTATTAATAGTTATTTTTTAATCGTCAAATATTATTAATTTAATAGATTGAGAACTTTTTGCTGGTATTTATAATTTCTGTCAATCGATCATCCATGAAAATGGATATTTTTTACTCTACTACTCAAAAATTAAGGAAACGATTATGTCAGCAAAATGCCCTTTCGATCATAACTCTGCCACATTGACTACCGCTGCCGGCGCGCCGGTGGTGGATAATGACAACACCATGAGTGCCGGCCCCCGCGGCCCGTTACTGTTACAAGATGTTTGGTATCAAGAAAAACTAGCCCACTTTGCCCGCGAGCGCATTCCTGAGCGTGTGGTGCACGCCAAGGGTTCCG  >  1/8854‑9332                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                       tAAACAATTTGAGCCCTGTGCAGTGCAGAGCTCAATATTTAACTTAGGTTTAGCTATCTGCCCGGTTTTAGGGTGCAGATCAAAGTGCGATCATTTTCTTTTCTCATTTCTTTTTTCTTGATTAAATCAATTCTAACTATTAATAGTTATTTTTTAATCGTCAAATATTATTAATTTAATAGATTGAGAACTTTTTGCTGGTATTTATAATTTCTGTCAATCGATCATCCATAAAAATGGATATTTTTTAc                                                                                                                                                                                                                                      <  1:575162/251‑1 (MQ=255)             gCCCTGTGCAGTGCAGAGCTCAATATTTAACTTAGGTTTAGCTATCTGCCCGGTTTTAGGGTGCAGATCAAAGTGCGATCATTTTCTTTTCTCATTTCTTTTTTCTTGATTAAATCAATTCTAACTATTAATAGTTATTTTTTAATCGTCAAATATTATTAATTTAATAGATTGAGAACTTTTTGCTGGTATTTATAATTTCTGTCAATCGATCATCCATAAAAATGGATATTTTTTACTCTACTACTCaa                                                                                                                                                                                                                          >  1:110976/1‑251 (MQ=255)                  gtgcagtgcaGAGCTCAATATTTAACTTAGGTTTAGCTATCTGCCCGGTTTTAGGGTGCAGATCAAAGTGCGATCATTTTCTTTTCTCATTTCTTTTTTCTTGATTAAATCAATTCTAACTATTAATAGTTATTTTTTAATCGTCAAATATTATTAATTTAATAGATTGAGAACTTTTTGCTGGTATTTATAATTTCTGTCAATCGATCATCCATAAAAATGGATATTTTTTACTCTACTACTCAAAAAtt                                                                                                                                                                                                                     >  1:287428/1‑251 (MQ=255)                       gtgcagAGCTCAATATTTAACTTAGGTTTAGCTATCTGCCCGGTTTTAGGGTGCAGATCAAAGTGCGATCATTTTCTTTTCTCATTTCTTTTTTCTTGATTAAATCAATTCTAACTATTAATAGTTATTTTTTAATCGTCAAATATTATTAATTTAATAGATTGAGAACTTTTTGCTGGTATTTATAATTTCTGTCAATCGATCATCCATAAAAATGGATATTTTTTACTCTACTACTc                                                                                                                                                                                                                            >  1:111448/1‑239 (MQ=255)                                   atatTTAACTTAGGTTTAGCTATCTGCCCGGTTTTAGGGTGCAGATCAAAGTGCGATCATTTTCTTTTCTCATTTCTTTTTTCTTGATTAAATCAATTCTAACTATTAATAGTTATTTTTTAATCGACAAATATTATTAATTTAATAGATTGAGAACTTTTTGCTGGTATTTATAATTTCTGTCAATCGATCATCCATAAAAATGGATATTTTTTACTCTACTACTCAAAAATTAAGGAAACGATTATGt                                                                                                                                                                                                     >  1:194101/1‑250 (MQ=255)                                     atTTAACTTAGGTTTAGCTATCTGCCCGGTTTTAGGGTGCAGATCAAAGTGCGATCATTTTCTTTTCTCATTTCTTTTTTCTTGATTAAATCAATTCTAACTATTAATAGTTATTTTTTAATCGTCAAATATTATTAATTTAATAGATTGAGAACTTTTTGCTGGTATTTATAATTTCTGTCAATCGATCATCCATAAAAATGGATAtttt                                                                                                                                                                                                                                          <  1:342551/211‑1 (MQ=255)                                          aCTTAGGTTTAGCTATCTGCCCGGTTTTAGGGTGCAGATCAAAGTGCGATCATTTTCTTTTCTCATTTCTTTTTTCTTGATTAAATCAATTCTAACTATTAATAGTTATTTTTTAATCGTCAAATATTATTAATTTAATAGATTGAGAACTTTTTGCTGGTATTTATAATTTCTGTCAATCGATCATCCATAAAAATGGATATTTTTTACTCTACTACTCAAAAATTAAgg                                                                                                                                                                                                                 >  1:529649/1‑231 (MQ=255)                                                                 gTTTTAGGGTGCAGATCAAAGTGCGATCATTTTCTTTTCTCATTTCTTTTTTCTTGATTAAATCAATTCTAACTATTAATAGTTATTTTTTAATCGTCAAATATTATTAATTTAATAGATTGAGAACTTTTTGCTGGTATTTATAATTTCTGTCAATCGATCATCCATAAAAATGGATATTTTTTACTCTACTACTCAAAAATTAAGGAAACGATTATGTCAGCAAAATGCCCTTTCGATCATAAc                                                                                                                                                                           <  1:867684/246‑1 (MQ=255)                                                                        ggTGCAGATCAAAGTGCGATCATTTTCTTTTCTCATTTCTTTTTTCTTGATTAAATCAATTCTAACTATTAATAGTTATTTTTTAATCGTCAAATATTATTAATTTAATAGATTGAGAACTTTTTGCTGGTATTTATAATTTCTGTCAATCGATCATCCATAAAAATGGATATTTTTTACTCTACTACTCAAAAATTAAGGAAACGATTATGTCAGCAAAATGCCCTTTCGATCATAACTCTGCCACAt                                                                                                                                                                 <  1:605270/249‑1 (MQ=255)                                                                                tCAAAGTGCGATCATTTTCTTTTCTCATTTCTTTTTTCTTGATTAAATCAATTCTAACTATTAATAGTTATTTTTTAATCGTCAAATATTATTAATTTAATAGATTGAGAACTTTTTGCTGGTATTTATAATTTCTGTCAATCGATCATCCATAAAAATGGATATTTTTTACTCTACTACTCAAAAATTAAGGAAACGATTATGTCAGCAAAATGCCCTTTCGATCATAActc                                                                                                                                                                         <  1:436063/233‑1 (MQ=255)                                                                                                      tctcATTTCTTTTTTCTTGATTAAATCAATTCTAACTATTAATAGTTATTTTTTAATCGTCAAATATTATTAATTTAATAGATTGAGAACTTTTTGCTGGTATTTATAATTTCTGTCAATCGATCATCCATAAAAATGGATATTTTTTACTCTACTACTCAAAAATTAAGGAAACGATTATGTCAGCAAAATGCCCTTTCGATCATAACTCTGCCACATTGACTACCGCTGCCGGCGCGCCGGTGGTGGAt                                                                                                                                 <  1:702290/251‑1 (MQ=255)                                                                                                             tCTTTTTTCTTGATTAAATCAATTCTAACTATTAATAGTTATTTTTTAATCGTCAAATATTATTAATTTAATAGATTGAGAACTTTTTGCTGGTATTTATAATTTCTGTCAATCGATCATCCATAAAAATGGATATTTTTTACTCTACTACTCAAAAATTAAGGAAACGATTATGTCAGCAAAATGCCCTTTCGATCATAACTCTGCCACATTGACTACCGCTGCCGGCGCGCCGGTGGTGGATAATGaca                                                                                                                          <  1:81290/251‑1 (MQ=255)                                                                                                                                cAATTCTAACTATTAATAGTTATTTTTTAATCGTCAAATATTATTAATTTAATAGATTGAGAACTTTTTGCTGGTATTTATAATTTCTGTCAATCGATCATCCATAAAAATGGATATTTTTTACTCTACTACTCAAAAATTAAGGAAACGATTATGTCAGCAAAATGCCCTTTCGATCATAACTCTGCCACATTGACTACCGCTGCCGGCGCGCCGGTGGTGGATAATGACAACACCATGAGTg                                                                                                              <  1:352902/244‑1 (MQ=255)                                                                                                                                         cTATTAATAGTTATTTTTTAATCGTCAAATATTATTAATTTAATAGATTGAGAACTTTTTGCTGGTATTTATAATTTCTGTCAATCGATCATCCATAAAAATGGATATTTTTTACTCTACTACTCAAAAATTAAGGAAACGATTATg                                                                                                                                                                                                      >  1:53398/1‑147 (MQ=255)                                                                                                                                                   ttATTTTTTAATCGTCAAATATTATTAATTTAATAGATTGAGAACTTTTTGCTGGTATTTATAATTTCTGTCAATCGATCATCCATAAAAATGGATATTTTTTACTCTACTACTCAAAAATTAAGGAAACGATTATGTCAGCAAAATGCCCTTTCGATCATAACTCTGCCACATTGACTACCGCTGCCGGCGCGCCGGTGGTGGATAATGACAACACCATGAGTGCCGGCCCCCGCGGCCCGTTACTGtt                                                                                     <  1:750846/250‑1 (MQ=255)                                                                                                                                                                       attattAATTTAATAGATTGAGAACTTTTTGCTGGTATTTATAATTTCTGTCAATCGATCATCCATAAAAATGGATATTTTTTACTCTACTACTCAAAAATTAAGGAAACGATTATGTCAGCAAAATGCCCTTTCGATCATAACTCTGCCACATTGACTACCGCTGCCGGCGCGCCGGTGGTGGATAATGACAACACCATGAGTGCCGGCCCCCGCGGCCCGTTACTGTTACAAGATGTTTGGTATCAAGa                                                                >  1:206480/1‑251 (MQ=255)                                                                                                                                                                          attaATTTAATAGATTGAGAACTTTTTGCTGGTATTTATAATTTCTGTCAATCGATCATCCATAAAAATGGATATTTTTTACTCTACTACTCAAAAATTAAGGAAACGATTATGTCAGCAAAATGCCCTTTCGATCATAAc                                                                                                                                                                           <  1:439477/141‑1 (MQ=255)                                                                                                                                                                            taatttaatAGATTGAGAACTTTTTGCTGGTATTTATAATTTCTGTCAATCGATCATCCATAAAAATGGATATTTTTTACTCTACTACTCAAAAATTAAGGAAACGATTATGTCAGCAAAATGCCCTTTCGATCATAACTCTGCCACATTGACTACCGCTGCCGGCGCGCCGGTGGTGGATAATGACAACACCATGAGTGCCGGCCCCCGCGGCCCGTTACTGTTACAAGATGTTTGGTATCAAGAAAAAc                                                           <  1:422199/251‑1 (MQ=255)                                                                                                                                                                             aatttaatAGATTGAGAACTTTTTGCTGGTATTTATAATTTCTGTCAATCGATCATCCATAAAAATGGATATTTTTTACTCCACTACTCAAAAATTAAGGAAACGATTATGTCAGCAAAATGCCCTTTCGATCATAACTCTGCCACATTGACTACCGCTGCCGGCGCGCCGGTGGTGGATAATGACAACACCATGAGTGCCGGCCCCCGCGGCCCGTTACTGTTACAAGATGTTTGGTATCAAGAAAAACt                                                          <  1:443021/251‑1 (MQ=255)                                                                                                                                                                              atttaatAGATTGAGAACTTTTTGCTGGTATTTATAATTTCTGTCAATCGATCATCCATAAAAATGGATATTTTTTACTCTACTACTCAAAAATTAAGGAAACGATTATGTCAGCAAAATGCCCTTTCGATCATAACTCTGCCACATTGACTACCGCTGCCGGCGCGCCGGTGGTGGATAATGac                                                                                                                           <  1:549078/185‑1 (MQ=255)                                                                                                                                                                              atttaatAGATTGAGAACTTTTTGCTGGTATTTATAATTTCTGTCAATCGATCATCCATAAAAATGGATATTTTTTACTCTACTACTCAAAAATTAAGGAAACGATTATGTCAGCAAAATGCCCTTTCGATCATAACTCTGCCACATTGACTACCGCTGCCGGCGCGCCGGTGGTGGATAATGACAACACCATGAGTGCCGGCCCCCGCGGCCCGTTACTGTTACAAGATGTTTGGTATCAAGAAAAAc                                                           >  1:618687/1‑249 (MQ=255)                                                                                                                                                                              atttaatAGATTGAGAACTTTTTGCTGGTATTTATAATTTCTGTCAATCGATCATCCATAAAAATGGATATTTTTTACTCTACTACTCAAAAATTAAGGAAACGATTATGTCAGCAAAATGCCCTTTCGATCATAACTCTGCCACATTGACTACCGCTGCCGGCGCGCCGGTGGTGGATAATGACAACACCATGAGTGCCGGCCCCCGCGGCCCGTTACTGTTACAAGATGTTTGGTATCAAGAAAAACTa                                                         >  1:341093/1‑251 (MQ=255)                                                                                                                                                                              atttaatAGATTGAGAACTTTTTGCTGGTATTTATAATTTCTGTCAATCGATCATCCATAAAAATGGATATTTTTTACTCTACTACTCAAAAATTAAGGAAACGATTATGTCAGCAAAATGCCCTTTCGATCATAACTCTGCCACATTGACTACCGCTGCCGGCGCGCCAGTGGTGGATAATGACAACACCATGAGTGCCGGCCCCCGCGGCCCGTTACTGTTACAAGATGTTTGGTATCAAGAAAAACTa                                                         >  1:450016/1‑251 (MQ=255)                                                                                                                                                                                       aTTGAGAACTTTTTGCTGGTATTTATAATTTCTGTCAATCGATCATCCATAAAAATGGATATTTTTTACTCTACTACTCAAAAATTAAGGAAACGATTATGt                                                                                                                                                                                                     >  1:202214/1‑102 (MQ=255)                                                                                                                                                                                       aTTGAGAACTTTTTGCTGGTATTTATAATTTCTGTCAATCGATCATCCATAAAAATGGATATTTTTTACTCTACTACTCAAAAATTAAGGAAACGATTATGTCAGCAAAATGCCCTTTCGATCATAACTCTGCCACATTGACTACCGCTGCCGGCGCGCCGGTGGTGGATAATGACAACACCATGAGTGCCGGCCCCCGCGGCCCGTTACTGTTACAAGa                                                                               >  1:874750/1‑220 (MQ=255)                                                                                                                                                                                                          tATTTATAATTTCTGTCAATCGATCATCCATAAAAATGGATATTTTTTACTCTACTACTCAAAAATTAAGGAAACGATTATGTCAGCAAAATGCCCTTTCGATCATAACTCTGCCACATTGACTACCGCTGCCGGCGCGCCGGTGGTGGATAATGac                                                                                                                           >  1:5979/1‑157 (MQ=255)                                                                                                                                                                                                           aTTTATAATTTCTGTCAATCGATCATCCATAAAAATGGATATTTTTTACTCTACTACTCAAAAATTAAGGAAACGATTATGTCAGCAAAATGCCCTTTCGATCATAACTCTGCCACATTGACTACCGCTGCCGGCGCGCCGGTGGTGGATAATGACAACACCATGAGTGCCGGCCCCCGCGGCCCGTTAc                                                                                         <  1:404235/190‑1 (MQ=255)                                                                                                                                                                                                           aTTTATAATTTCTGTCAATCGATCATCCATAAAAATGGATATTTTTTACTCTACTACTCAAAAATTAAGGAAACGATTATGTCAGCAAAATGCCCTTTCGATCATAACTCTGCCACATTGACTACCGCTGCCGGCGCGCCGGTGGTGGATAATGACAACACCATGAGTGCCGGCCCCCGCGGCCCGTTACTGTTACAAGATg                                                                             >  1:649154/1‑202 (MQ=255)                                                                                                                                                                                                           aTTTATAATTTCTGTCAATCGATCATCCATAAAAATGGATATTTTTTACTCTACTACTCAAAAATTAAGGAAACGATTATGTCAGCAAAATGCCCTTTCGATCATAACTCTGCCACATTGACTACCGCTGCCGGCGCGCCGGTGGTGGATAATGACAACACCATGAGTGCCGGCCCCCGCGGCCCGTTACTGTTACAAGATGTTTGGTATCAAGAAAAACTAGccc                                                     >  1:333652/1‑226 (MQ=255)                                                                                                                                                                                                             ttATAATTTCTGTCAATCGATCATCCATAAAAATGGATATTTTTTACTCTACTACTCAAAAATTAAGGAAACGATTATGTCAGCAAAATGCCCTTTCGATCATAACTCTGCCACATTGACTACCGCTGCCGGCGCGCCGGTGGTGGATAATGACAACACCATGAGTGCCGGCCCCCGCGGCCCGTTACTGTTACAAGATGTTTGGTATCAAGAAAAACTAGCCCACTTTGCCCGCGAGCGCATTCCTGAg                           >  1:205535/1‑250 (MQ=255)                                                                                                                                                                                                                  aTTTCTGTCAATCGATCATCCATAAAAATGGATATTTTTTACTCTACTACTCAAAAATTAAGGAAACGATTATGTCAGCAAAATGCCCTTTCGATCATAACTCTGCCACATTGACTACCGCTGCCGGCGCGCCGGTGGTGGATAATGACAACACCATGAGTGCCGGCCCCCGCGGCCCGTTACTGTTACAAGATGTTTGGTATCAAGa                                                                >  1:156340/1‑208 (MQ=255)                                                                                                                                                                                                                        gTCAATCGATCATCCATAAAAATGGATATTTTTTACTCTACTACTCAAAAATTAAGGAAACGATTATGTCAGCAAAATGCCCTTTCGATCATAACTCTGCCACATTGACTACCGCTGCCGGCGCGCCGGTGGTGGATAATGACAACACCATGAGTGCCGGCCCCCGCGGCCCGTTACTGTTACAAGATGTTTGGTATCAAGAAAAACTAGCCCACTTTGCCCGCGAGCGCATTCCTGAGCGTGTGGTGCa                >  1:821610/1‑250 (MQ=255)                                                                                                                                                                                                                           aaTCGATCATCCATAAAAATGGATATTTTTTACTCTACTACTCAAAAATTAAGGAAACGATTATGTCAGCAAAATGCCCTTTCGATCATAACTCTGCCACATTGACTACCGCTGCCGGCGCGCCGGTGGTGGATAATGACAACACCATGAGTGCCGGCCCCCGCGGCCCGTTACTGTTACAAGATGTTTGGTATCAAGAAAAACTAGCCCACTTTGCCCGCGAGCGCATTCCTGAGCGTGTGGTGCACGcc            <  1:76975/251‑1 (MQ=255)                                                                                                                                                                                                                            aTCGATCATCCATAAAAATGGATATTTTTTACTCTACTACTCAAAAATTAAGGAAACGATTATGTCAGCAAAATGCCCTTTCGATCATAACTCTGCCACATTGACTACCGCTGCCGGCGCGCCGGTGGTGGATAATGACAACACCATGAGTGCCGGCCCCCGCGGCCCGTTACTGTTACAAGATGTTTGGTATCAAGAAAAACTAGCCCACTTTGCCCGCGAGCGCATTCCTGAGCGTGTGGTGCACGCCa           >  1:450983/1‑251 (MQ=255)                                                                                                                                                                                                                                     ccATAAAAATGGATATTTTTTACTCTACTACTCAAAAATTAAGGAAACGATTATGTCAGCAAAATGCCCTTTCGATCATAACTCTGCCACATTGACTACCGCTGCCGGCGCGCCGGTGGTGGATAATGACAACACCATGAGTGCCGGCCCCCGCGGCCCGTTACTGTTACAAGATGTTTGGTATCAAGAAAAACTAGCCCACTTTGCCCGCGAGCGCATTCCTg                             <  1:423481/224‑1 (MQ=255)                                                                                                                                                                                                                                     ccATAAAAATGGATATTTTTTACTCTACTACTCAAAAATTAAGGAAACGATTATGTCAGCAAAATGCCCTTTCGATCATAACTCTGCCACATTGACTACCGCTGCCGGCGCGCCGGTGGTGGATAATGACAACACCATGAGTGCCGGCCCCCGCGGCCCGTTACTGTTACAAGATGTTTGGTATCAAGAAAAACTAGCCCACTTTGCCCGCGAGCGCATTCCTGAGCGTGTGGTGCACGCCAAGGGTTccg  >  1:202122/1‑251 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                       TAAACAATTTGAGCCCTGTGCAGTGCAGAGCTCAATATTTAACTTAGGTTTAGCTATCCGCCCGGTTTTAGGGTGCAGATCAAAGTGCGATCATTTTCTTTTCTCATTTCTTTTTTCTTGATTAAATCAATTCTAACTATTAATAGTTATTTTTTAATCGTCAAATATTATTAATTTAATAGATTGAGAACTTTTTGCTGGTATTTATAATTTCTGTCAATCGATCATCCATGAAAATGGATATTTTTTACTCTACTACTCAAAAATTAAGGAAACGATTATGTCAGCAAAATGCCCTTTCGATCATAACTCTGCCACATTGACTACCGCTGCCGGCGCGCCGGTGGTGGATAATGACAACACCATGAGTGCCGGCCCCCGCGGCCCGTTACTGTTACAAGATGTTTGGTATCAAGAAAAACTAGCCCACTTTGCCCGCGAGCGCATTCCTGAGCGTGTGGTGCACGCCAAGGGTTCCG  >  1/8854‑9332

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑