Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,330,606	C→T	intergenic (+191476/+62705)	unknown → / ← unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,330,606	0	C	T	100.0%	195.9 / NA	68	intergenic (+191476/+62705)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/39);  total (29/39)

CTTGTTTGGCTTCGTTAATTTTTGCACCCACCGCCGGACGCTCTTCCGCCGCCACATTGCGTAGTTCTTGCATTAATTGGGTAAAATGCCCTTTTTTACC‑AAAATATTCCACCCGAATTTCATCAAGTGCCGCCAGGCTTTTATTTTCAATACTGTCTATAGCATTTCTTGCTTTCTCCACAAGATCTTTAAGATGCTGCATATATATCCTCTGTCATGTTCGATTTATCTAAAAAAATGCCTGCAATGATAATATTAACTGCTTAGAAAATCACGCAATTTATCACGTTTTACGCAAATTATTCGTGAGTAGAAAAGGATACCGATAAAAAACGCCCGAAAAAATTATTTACAAACTCGCTCTCTCGTTTCTTCACCCCTAACGGGCGGTTGGAAAAGCCAACGTTCAAAAAACGTTGTTTTTTGTGACCGCACTTTTTGTCCTGCAGGAAATTTTAGTGCTGAATCTATTAAGCTATTTTTCATAGACA  >  1/1330362‑1330852                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       cTTGTTTGGCTTCGTTAATTTTTGCACCCACCGCCGGACGCTCTTCCGCCGCCACATTGCGTAGTTCTTGCATTAATTGGGTAAAATGCCCTTTTTTACC‑AAAATATTCCACCCGAATTTCATCAAGTGCCGCCAGGCTTTTATTTTCAATACTGTCTATAGCATTTCTTGCTTTCTCCACAAGATCTTTAAGATGCTGCATATATATCCTCTGTCATGTTCGATTTATCTAAAAAAATGCCTGTAATGa                                                                                                                                                                                                                                                   <  1:818119/250‑1 (MQ=255)  ttGTTTGGCTTCGTTAATTTTTGCACCCACCGCCGGACGCTCTTCCGCCGCCACATTGCGTAGTTCTTGCATTAATTGGGTAAAATGCCCTTTTTTACC‑AAAATATTCCACCCGAATTTCATCAAGTGCCGCCAGGCTTTTATTTTCAATACTGTCTATAGCATTTCTTGCTTTCTCCACAAGATCTTTAAGATGCTGCATATATATCCTCTGTCATGTTCGATTTATCTAAAAAAATGCCTGTAATGata                                                                                                                                                                                                                                                 <  1:767468/251‑1 (MQ=255)            tCGTTAATTTTTGCACCCACCGCCGGACGCTCTTCCGCCGCCACATTGCGTAGTTCTTGCATTAATTGGGTAAAATGCCCTTTTTTACC‑AAAATATTCCACCCGAATTTCATCAAGTGCCGCCAGGCTTTTATTTTCAATACTGTCTATAGCATTTCTTGCTTTCTCCACAAGATCTTTAAGATGCTGCATATATATCCTCTGTCATGTTCGATTTATCTAAAAAAATGCCTGTAATGATAATATTAACTg                                                                                                                                                                                                                                       <  1:39676/251‑1 (MQ=255)            tCGTTAATTTTTGCACCCACCGCCGGACGCTCTTCCGCCGCCACATTGCGTAGTTCTTGCATTAATTGGGTAAAATGCCCTTTTTTACC‑AAAATATTCCACCCGAATTTCATCAAGTGCCGCCAGGCTTTTATTTTCAATACTGTCTATAGCATTTCTTGCTTTCTCCACAAGATCTTTAAGATGCTGCATATATATCCTCTGTCATGTTCGATTTATCTAAAAAAATGCCTGTAATGATAATATTAACTg                                                                                                                                                                                                                                       <  1:469405/251‑1 (MQ=255)                   tttttGCACCCACCGCCGGACGCTCTTCCGCCGCCACATTGCGTAGTTCTTGCATTAATTGGGTAAAATGCCCTTTTTTACCAAAAATATTCCACCCGAATTTCATCAAGTGCCGCCAGGCTTTTATTTTCAATACTGTCTATAGCATTTCTTGCTTTCTCCACAAGATCTTTAAGATGCTGCATATATATCCTCTGTCATGTTCGATTTATCTAAAAAAATGCCTGTAATGATAATATTAACTGCTTAGa                                                                                                                                                                                                                                 >  1:136832/1‑251 (MQ=255)                   tttttGCACCCACCGCCGGACGCTCTTCCGCCGCCACATTGCGTAGTTCTTGCATTAATTGGGTAAAATGCCCTTTTTTACC‑AAAATATTCCACCCGAATTTCATCAAGTGCCGCCAGGCTTTTATTTTCAATACTGTCTATAGCATTTCTTGCTTTCTCCACAAGATCTTTAAGATGCTGCATATATATCCTCTGTCATGTTCGATTTATCTAAAAAAATGCCTGTAATGATAATATTAACTg                                                                                                                                                                                                                                       <  1:304064/244‑1 (MQ=255)                                         ctctTCCGCCGCCACATTGCGTAGTTCTTGCATTAATTGGGTAAAATGCCCTTTTTTACC‑AAAATATTCCACCCGAATTTCATCAAGTGCCGCCAGGCTTTTATTTTCAATACTGTCTATAGCATTTCTTGCTTTCTCCACAAGATCTTTAAGATGCTGCATATATATCCTCTGTCATGTTCGATTTATCTAAAAAAATGCCTGTAATGATAATATTAACTGCTTAGAAAATCACGCAATTTATCACGttt                                                                                                                                                                                                          >  1:200448/1‑251 (MQ=255)                                                           gCGTAGTTCTTGCATTAATTGGGTAAAATGCCCTTTTTTACC‑AAAATATTCCACCCGAATTTCATCAAGTGCCGCCAGGCTTTTATTTTCAATACTGTCTATAGCATTTCTTGCTTTCTCCACAAGATCTTTAAGATGCTGCATATATATCCTCTGTCATGTTCGATTTATCTAAAAAAATGCCTGTAATGATAATATTAACTGCTTAGAAAATCACGCAATTTATCACGTTTTACGCAAATTATTCGTGa                                                                                                                                                                                        >  1:628910/1‑251 (MQ=255)                                                                gTTCTTGCATTAATTGGGTAAAATGCCCTTTTTTACC‑AAAATATTCCACCCGAATTTCATCAAGTGCCGCCAGGCTTTTATTTTCAATACTGTCTATAGCATTTCTTGCTTTCTCCACAAGATCTTTAAGATGCTGCATATATATCCTCTGTCATGTTCGATTTATCTAAAAAAATGCCTGTAATGATAATATTAACTGCTTAGAAAATCACGCAATTTATCACGTTTTACGCAAATTATTCGTGAGTAGa                                                                                                                                                                                   >  1:202493/1‑251 (MQ=255)                                                                         ttAATTGGGTAAAATGCCCTTTTTTACC‑AAAATATTCCACCCGAATTTCATCAAGTGCCGCCAGGCTTTTATTTTCAATACTGTCTATAGCATTTCTTGCTTTCTCCACAAGATCTTTAAGATGCTGCATATATATCCTCTGTCATGTTCGATTTATCTAAAAAAATGCCTGTAATGATAATATTAACTGCTTAGAAAATCACGCAATTTATCACGTTTTACGCAAATTATTCGTGAGTAGAAAAGGATAc                                                                                                                                                                          <  1:584780/251‑1 (MQ=255)                                                                            aTTGGGTAAAATGCCCTTTTTTACC‑AAAATATTCCACCCGAATTTCATCAAGTGCCGCCAGGCTTTTATTTTCAATACTGTCTATAGCATTTCTTGCTTTCTCCACAAGATCTTTAAGATGCTGCATATATATCCTCTGTCATGTTCGATTTATCTAAAAAAATGCCTGTAATGATAATATTAACTGCTTAGAAAATCACGCAATTTATCACGTTTTACGCAAATTATTCGTGAGTAGAAAAGGATACCGa                                                                                                                                                                       <  1:289657/251‑1 (MQ=255)                                                                               gggTAAAATGCCCTTTTTTACC‑AAAATATTCCACCCGAATTTCATCAAGTGCCGCCAGGCTTTTATTTTCAATACTGTCTATAGCATTTCTTGCTTTCTCCACAAGATCTTTAAGATGCTGCATATATATCCTCTGTCATGTTCGATTTATCTAAAAAAATGCCTGTAATGATAATATTAACTGCTTAGAAAATCACGCAATTTATCACGTTTTACGCAAATTATTCGTGAGTAGAAAAGGATACCGATaa                                                                                                                                                                    >  1:144470/1‑251 (MQ=255)                                                                                                      aaaaTATTCCACCCGAATTTCATCAAGTGCCGCCAGGCTTTTATTTTCAATACTGTCTATAGCATTTCTTGCTTTCTCCACAAGATCTTTAAGATGCTGCATATATATCCTCTGTCATGTTCGATTTATCTAAAAAAATGCCTGTAATGATAATATTAACTGCTTAGAAAATCACGCAATTTATCACGTTTTACGCAAATTATTCGTGAGTAGAAAAGGATACCGATAAAAAACGCCCGAAAAAATTAttt                                                                                                                                              >  1:832954/1‑251 (MQ=255)                                                                                                                            tcaAGTGCCGCCAGGCTTTTATTTTCAATACTGTCTATAGCATTTCTTGCTTTCTCCACAAGATCTTTAAGATGCTGCATATATATCCTCTGTCATGTTCGATTTATCTAAAAAAATGCCTGTAATGATAATATTAACTGCTTAGAAAATCACGCAATTTATCACGTTTTACGCAAATTATTCGTGAGTAGAAAAGGATACCGATAAAAAACGCCCGAAAAAATTATTTACAAACTCGCTCTCTCGTttct                                                                                                                        <  1:349602/251‑1 (MQ=255)                                                                                                                                   ccgccAGGCTTTTATTTTCAATACTGTCTATAGCATTTCTTGCTTTCTCCACAAGATCTTTAAGATGCTGCATATATATCCTCTGTCATGTTCGATTTATCTAAAAAAATGCCTGTAATGATAATATTAACTGCTTAGAAAATCACGCAATTTATCACGTTTTACGCAAATTATTCGTGAGTAGAAAAGGATACCGATAAAAAACGCCCGAAAAAATTATTTACAAACTCGCTCTCTCGTTTCTTCAccc                                                                                                                  <  1:374211/250‑1 (MQ=255)                                                                                                                                          gCTTTTATTTTCAATACTGTCTATAGCATTTCTTGCTTTCTCCACAAGATCTTTAAGATGCTGCATATATATCCTCTGTCATGTTCGATTTATCTAAAAAAATGCCTGTAATGATAatat                                                                                                                                                                                                                                             <  1:590556/120‑1 (MQ=255)                                                                                                                                                 ttttCAATACTGTCTATAGCATTTCTTGCTTTCTCCACAAGATCTTTAAGATGCTGCATATATATCCTCTGTCATGTTCGATTTATCTAAAAAAATGCCTGTAATGATAATATTAACTGCTTAGAAAATCACGCAATTTATCACGTTTTACGCAAATTATTCGTGAGTAGAAAAGGATACCGATAAAAAACGCCCGAAAAAATTATTTACAAACTCGCTCTCTCGTTTCTTCACCCCTAACGGGCGGTTgg                                                                                                   >  1:53535/1‑251 (MQ=255)                                                                                                                                                  tttCAATACTGTCTATAGCATTTCTTGCTTTCTCCACAAGATCTTTAAGATGCTGCATATATATCCTCTGTCATGTTCGATTTATCTAAAAAAATGCCTGTAATGATAATATTAACTGCTTAGAAAATCACGCAATTTATCACGTTTTACGCAAATTATTCGTGAGTAGAAAAGGATACCGATAAAAAACGCCCGAAAAAATTATTTACAAACTCGCTCTCTCGTTTCTTCACCCCTAACGGGCGGTTGGa                                                                                                  >  1:558583/1‑251 (MQ=255)                                                                                                                                                      aaTACTGTCTATAGCATTTCTTGCTTTCTCCACAAGATCTTTAAGATGCTGCATATATATCCTCTGTCATGTTCGATTTATCTAAAAAAATGCCTGTAATGATAATATTAACTGCTTAGAAAATCACGCAATTTATCACGTTTTACGCAAATTATTCGTGAGTAGAAAAGGATACCGATAAAAAACGCCCGAAAAAATTATTTACAAACTCGCTCTCTCGTTTCTTCACCCCTAACGGGCGGTTGGAAAAg                                                                                              <  1:141308/251‑1 (MQ=255)                                                                                                                                                        tACTGTCTATAGCATTTCTTGCTTTCTCCACAAGATCTTTAAGATGCTGCATATATATCCTCTGTCATGTTCGATTTATCTAAAAAAATGCCTGTAATGATAATATTAACTGCTTAGAAAATCACGCAATTTATCACGTTTTACGCAAATTATTCGTGAGTAGAAAAGGATACCGATAAAAAACGCCCGAAAAAATTATTTACAAACTCGCTCTCTCGTTTCTTCACCCCTAACGGGCGGTTGGAAAAGcc                                                                                            <  1:791388/251‑1 (MQ=255)                                                                                                                                                            gTCTATAGCATTTCTTGCTTTCTCCACAAGATCTTTAAGATGCTGCATATATATCCTCTGTCATGTTCGATTTATCTAAAAAAATGCCTGTAATGATAATATTAACTGCTTAGAAAATCACGCAATTTATCACGTTTTACGCAAATTATTCGTGAGTAGAAAAGGATACCGa                                                                                                                                                                       <  1:809423/172‑1 (MQ=255)                                                                                                                                                            gTCTATAGCATTTCTTGCTTTCTCCACAAGATCTTTAAGATGCTGCATATATATCCTCTGTCATGTTCGATTTATCTAAAAAAATGCCTGTAATGATAATATTAACTGCTTAGAAAATCACGCAATTTATCACGTTTTACGCAAATTATTCGTGAGTAGAAAAGGATACCGATAAAAAACGCCCGAAAAAATTATTTACAAACTCGCTCTCTCGTTTCTTCACCCCTAACGGGCGGTTGGAAAAGCCAACg                                                                                        >  1:18137/1‑251 (MQ=255)                                                                                                                                                              cTATAGCATTTCTTGCTTTCTCCACAAGATCTTTAAGATGCTGCATATATATCCTCTGTCATGTTCGATTTATCTAAAAAAATGCCTGTAATGATAATATTAACTGCTTAGAAAATCACGCAATTTATCACGTTTTACGCAAatta                                                                                                                                                                                               >  1:222594/1‑146 (MQ=255)                                                                                                                                                              cTATAGCATTTCTTGCTTTCTCCACAAGATCTTTAAGATGCTGCATATATATCCTCTGTCATGTTCGATTTATCTAAAAAAATGCCTGTAATGATAATATTAACTGCTTAGAAAATCACGCAATTTATCACGTTTTACGCAAATTATTCg                                                                                                                                                                                           >  1:340156/1‑150 (MQ=255)                                                                                                                                                              cTATAGCATTTCTTGCTTTCTCCACAAGATCTTTAAGATGCTGCATATATATCCTCTGTCATGTTCGATTTATCTAAAAAAATGCCTGTAATGATAATATTAACTGCTTAGAAAATCACGCAATTTATCACGTTTTACGCAAATTATTCGTGAGTAGAAAAGGATACCGATAAAAAACGCCCGAAAAAATTATTTACAAACTCGCTCTCTCGTTTCTTCAcc                                                                                                                   >  1:88954/1‑222 (MQ=255)                                                                                                                                                                              tttCTCCACAAGATCTTTAAGATGCTGCATATATATCCTCTGTCATGTTCGATTTATCTAAAAAAATGCCTGTAATGATAATATTAACTGCTTAGAAAATCACGCAATTTATCACGTTTTACGCAAATTATTCGTGAGTAGAAAAGGATACCGATAAAAAACGCCCGAAAAAATTATTTACAAACTCGCTCTCTCGTttct                                                                                                                        <  1:679101/201‑1 (MQ=255)                                                                                                                                                                                 ctcCACAAGATCTTTAAGATGCTGCATATATATCCTCTGTCATGTTCGATTTATCTAAAAAAATGCCTGTAATGATAATATTAACTGCTTAGAAAATCACGCAATTTATCACGTTTTACGCAAATTATTCGTGAGTAGAAAAGGATACCGATAAAAAACGCCCGAAAAAATTATTTACAAACTCGCTCTCTCGTTTCTTCACCCCTAACGGGCGGTTGGAAAAGCCAACGTTCAAAAAACGTTGTTTTTtg                                                                   <  1:482771/251‑1 (MQ=255)                                                                                                                                                                                 ctcCACAAGATCTTTAAGATGCTGCATATATATCCTCTGTCATGTTCGATTTATCTAAAAAAATGCCTGTAATGATAATATTAACTGCTTAGAAAATCACGCAATTTATCACGTTTTACGCAAATTATTCGTGAGTAGAAAAGGATACCGATAAAAAACGCCCGAAAAAATTATTTACAAACTCGCTCTCTCGTTTCTTCACCCCTAACGGGCGGTTGGAAAAGCCAACGTTCAAAAAACGTTGTTTTTtg                                                                   >  1:333915/1‑251 (MQ=255)                                                                                                                                                                                            cTTTAAGATGCTGCATATATATCCTCTGTCATGTTCGATTTATCTAAAAAAATGCCTGTAATGATAATATTAACTGCTTAGAAAATCACGCAATTTATCACGTTTTACGCAAATTATTCGTGAGTAGAAAAGGATACCGATAAAAAACGCCCGAAAAAATTATTTACAAACTCGCTCTCTCGTTTCTTCACCCCTAACGGGCGGTTGGAAAAGCCAACGTTCaaaaa                                                                                <  1:79790/227‑1 (MQ=255)                                                                                                                                                                                              ttAAGATGCTGCATATATATCCTCTGTCATGTTCGATTTATCTAAAAAAATGCCTGTAATGATAATATTAACTGCTTAGAAAATCACGCAATTTATCACGTTTTACGCAAATTATTCGTGAGTAGAAAAGGATACCGATAAAAAACGCCCGAAAAAATTATTTACAAACTCGCTCTCTCGTTTCTTCACCCCTAACg                                                                                                            <  1:557321/197‑1 (MQ=255)                                                                                                                                                                                                aaGATGCTGCATATATATCCTCTGTCATGTTCGATTTATCTAAAAAAATGCCTGTAATGATAATATTAACTGCTTAGAAAATCACGCAATTTATCACGTTTTACGCAAATTATTCGTGAGTAGAAAAGGATACCGATAAAAAACGCCCGAAAAAATTATTTACAAACTCGCTCTCTCGTTTCTTCACCCCTAACGGGCGGTTGGAAAAGCCAACGTTCAAAAAACGTTGTTTTTTGTGACCGCACTTTTTg                                                    <  1:637981/251‑1 (MQ=255)                                                                                                                                                                                                   aTGCTGCATATATATCCTCTGTCATGTTCGATTTATCTAAAAAAATGCCTGTAATGATAATATTAACTGCTTAGAAAATCACGCAATTTATCACGTTTTACGCAAATTATTCGTGAGTAGAAAAGGATACCGATAAAAAACGCCCGAAAAAATTATTTACAAACTCGCTCTCTCGTTTCTTCACCCCTAACGGGCGGTTGGAAAAGCCAACGTTCAAAAAACGTTGTTTTTTGTGACCGCACTTTTTGTcc                                                 <  1:615186/251‑1 (MQ=255)                                                                                                                                                                                                      ctgtatatatatCCTCTGTCATGTTCGATTTATCTAAAAAAATGCCTGTAATGATAATATTAACTGCTTAGAAAATCACGCaa                                                                                                                                                                                                                      <  1:73240/79‑1 (MQ=255)                                                                                                                                                                                                        gcagatatatCCTCTGTCATGTTCGATTTATCTAAAAAAATGCCTGTAATGATAATATTAACTGCTTAGAAAATCACGCAATTTATCACGTTTTACGCAAATTATTCGTGAGTAGAAAAGGATACCGATAAAAAACGCCCGAAAAAATTATTTACAAACTCGCTCTCTCGTTTCTTCACCCCTAACGGGCGGTTGGAAAAGCCAACGTTCAAAAAACGTTGTTTTTTGTGACCGCACTTTTTGTCCTGCAg                                            >  1:462635/5‑251 (MQ=255)                                                                                                                                                                                                        gcATATATATCCTCTGTCATGTTCGATTTATCTAAAAAAATGCCTGTAATGATAATATTAACTGCTTAGAAAATCACGCAATTTATCACGTTTTACGCAAATTATTCGTGAGTAGAAAAGGATACCGATAAAAAACGCCCGAAAAAATTATTTACAAACTCGCTCTCTCGTttct                                                                                                                        <  1:770627/175‑1 (MQ=255)                                                                                                                                                                                                        gcATATATATCCTCTGTCATGTTCGATTTATCTAAAAAAATGCCTGTAATGATAATATTAACTGCTTAGAAAATCACGCAATTTATCACGTTTTACGCAAATTATTCGTGAGTAGAAAAGGATACCGATAAAAAACGCCCGAAAAAATTATTTACAAACTCGCTCTCTCGTTTCTTCACCCCTAACGGGCGGTTGGAAAAGCCAACGTTCAAAAAACGTTGTTTTTtg                                                                   >  1:363261/1‑228 (MQ=255)                                                                                                                                                                                                        gcATATATATCCTCTGTCATGTTCGATTTATCTAAAAAAATGCCTGTAATGATAATATTAACTGCTTAGAAAATCACGCAATTTATCACGTTTTACGCAAATTATTCGTGAGTAGAAAAGGATACCGATAAAAAACGCCCGAAAAAATTATTTACAAACTCGCTCTCTCGTTTCTTCACCCCTAACGGGCGGTTGGAAAAGCCAACGTTCAAAAAACGTTGTTTTTtg                                                                   <  1:316133/228‑1 (MQ=255)                                                                                                                                                                                                        gcATATATATCCTCTGTCATGTTCGATTTATCTAAAAAAATGCCTGTAATGATAATATTAACTGCTTAGAAAATCACGCAATTTATCACGTTTTACGCAAATTATTCGTGAGTAGAAAAGGATACCGATAAAAAACGCCCGAAAAAATTATTTACAAACTCGCTCTCTCGTTTCTTCACCCCTAACGGGCGGTTGGAAAAGCCAACGTTCAAAAAACGTTGTTTTTTGTGACCGCACTTTTTGTCCTGCAg                                            >  1:771457/1‑251 (MQ=255)                                                                                                                                                                                                        gcATATATATCCTCTGTCATGTTCGATTTATCTAAAAAAATGCCTGTAATGATAATATTAACTGCTTAGAAAATCACGCAATTTATCACGTTTTACGCAAATTATTCGTGAGTAGAAAAGGATACCGATAAAAAACGCCCGAAAAAATTATTTACAAACTCGCTCTCTCGTTTCTTCACCCCTAACGGGCGGTTGGAAAAGCCAACGTTCAAAAAACGTTGTTTTTTGTGACCGCACTTTTTGTCCTGCAg                                            >  1:315527/1‑251 (MQ=255)                                                                                                                                                                                                          atatatatCCTCTGTCATGTTCGATTTATCTAAAAAAATGCCTGTAATGATAATATTAACTGCTTAGAAAATCACGCAATTTATCACGTTTTACGCAAATTATTCGTGAGTAGAAAAGGATACCGATAAAAAACGCCCGAAAAAATTATTTACAAACTCGCTCTCTCGTTTCTTCACCCCTAACGGGCGGTTGGAAAAGCCAACGTTCAAAAAACGTTGTTTTTTGTGACCGCACTTTTTGTCCTGCAGGa                                          >  1:495392/1‑251 (MQ=255)                                                                                                                                                                                                           tatatatCCTCTGTCATGTTCGATTTATCTAAAAAAATGCCTGTAATGATAATATTAACTGCTTAGAAAATCACGCAATTTATCACGTTTTACGCAAATTATTCGTGAGTAGAAAAGGATAc                                                                                                                                                                          >  1:727349/1‑122 (MQ=255)                                                                                                                                                                                                           tatatatCCTCTGTCATGTTCGATTTATCTAAAAAAATGCCTGTAATGATAATATTAACTGCTTAGAAAATCACGCAATTTATCACGTTTTACGCAAATTATTCGTGAGTAGAAAAGGATACCGATAAAAAACGCCCGAAAAAATTATTTACAAACTCGCTCTCTCGTttct                                                                                                                        <  1:200196/172‑1 (MQ=255)                                                                                                                                                                                                           tatatatCCTCTGTCATGTTCGATTTATCTAAAAAAATGCCTGTAATGATAATATTAACTGCTTAGAAAATCACGCAATTTATCACGTTTTACGCAAATTATTCGTGAGTAGAAAAGGATACCGATAAAAAACGCCCGAAAAAATTATTTACAAACTCGCTCTCTCGTTTCTTCACCCCTAACGGGCGGTTGGAAAAGCCAACGTTCAAAAAACGTTGTTTTTtgtg                                                                 <  1:369345/227‑1 (MQ=255)                                                                                                                                                                                                           tatatatCCTCTGTCATGTTCGATTTATCTAAAAAAATGCCTGTAATGATAATATTAACTGCTTAGAAAATCACGCAATTTATCACGTTTTACGCAAATTATTCGTGAGTAGAAAAGGATACCGATAAAAAACGCCCGAAAAAATTATTTACAAACTCGCTCTCTCGTTTCTTCACCCCTAACGGGCGGTTGGAAAAGCCAACGTTCAAAAAACGTTGTTTTTTGTGACCGCACttt                                                       <  1:543495/237‑1 (MQ=255)                                                                                                                                                                                                           tatatatCCTCTGTCATGTTCGATTTATCTAAAAAAATGCCTGTAATGATAATATTAACTGCTTAGAAAATCACGCAATTTATCACGTTTTACGCAAATTATTCGTGAGTAGAAAAGGATACCGATAAAAAACGCCCGAAAAAATTATTTACAAACTCGCTCTCTCGTTTCTTCACCCCTAACGGGCGGTTGGAAAAGCCAACGTTCAAAAAACGTTGTTTTTTGTGACCGCACTTTTTg                                                    >  1:694711/1‑240 (MQ=255)                                                                                                                                                                                                           tatatatCCTCTGTCATGTTCGATTTATCTAAAAAAATGCCTGTAATGATAATATTAACTGCTTAGAAAATCACGCAATTTATCACGTTTTACGCAAATTATTCGTGAGTAGAAAAGGATACCGATAAAAAACGCCCGAAAAAATTATTTACAAACTCGCTCTCTCGTTTCTTCACCCCTAACGGGCGGTTGGAAAAGCCAACGTTCAAAAAACGTTGTTTTTTGTGACCGCACTTTTTGt                                                   >  1:270518/1‑241 (MQ=255)                                                                                                                                                                                                            atatatCCTCTGTCATGTTCGATTTATCTAAAAAAATGCCTGTAATGATAATATTAACTGCTTAGAAAATCACGCAATTTATCACGTTTTACGCAAATTATTCGTGAGTAGAAAAGGATACCGATAAAAAACGCCCGAAAAAATTATTTACAAACTCGCTCTCTCGTTTCTTCACCCCTAACGGGCGGTTGGAAAAGCCAACGTTCAAAAAACGTTGTTTTTTGTGACCGCACTTTTTGTcc                                                 >  1:216074/1‑242 (MQ=255)                                                                                                                                                                                                                  ccTCTGTCATGTTCGATTTATCTAAAAAAATGCCTGTAATGATAATATTAACTGCTTAGAAAATCACGCAATTTATCACGTTTTACGCAAATTATTCGTGAGTAGAAAAGGATACCGATAAAAAACGCCCGAAAAAATTATTTACAAACTCGCTCTCTCGTTTCTTCACCCCTAACGGGCGGTTGGAAAAGCCAACGTTCAAAAAACGTTGTTTTTTGTGACCGCACTTTTTGTCCTGCAGGAAATTTTAg                                  <  1:186725/251‑1 (MQ=255)                                                                                                                                                                                                                  ccTCTGTCATGTTCGATTTATCTAAAAAAATGCCTGTAATGATAATATTAACTGCTTAGAAAATCACGCAATTTATCACGTTTTACGCAAATTATTCGTGAGTAGAAAAGGATACCGATAAAAAACGCCCGAAAAAATTATTTACAAACTCGCTCTCTCGTTTCTTCACCCCTAACGGGCGGTTGGAAAAGCCAACGTTCAAAAAACGTTGTTTTTTGTGACCGCACTTTTTGTCCTGCAGGAAATTTTAg                                  <  1:66279/251‑1 (MQ=255)                                                                                                                                                                                                                    tctGTCATGTTCGATTTATCTAAAAAAATGCCTGTAATGATAATATTAACTGCTTAGAAAATCACGCAATTTATCACGTTTTACGCAAATTATTCGTGAGTAGAAAAGGATACCGATAAAAAACGCCCGAAAAAATTATTTACAAACTCGCTCTCTCGTTTCTTCACCCCTAACGGGCGGTTGGAAAAGCCAACGTTCAAAAAACGTTGTTTTTTGTGACCGCACTTTTTGTCCTGCAGGAAATTTTAGTg                                >  1:336508/1‑251 (MQ=255)                                                                                                                                                                                                                     ctGTCATGTTCGATTTATCTAAAAAAATGCCTGTAATGATAATATTAACTGCTTAGAAAATCACGCAATTTATCACGTTTTACGCAAATTATTCGTGAGTAGAAAAGGATACCGATAAAAAACGCCCGAAAAAATTATTTACAAACTCGCTCTCTCGTTTCTTCACCCCTAACGGGCGGTTGGAAAAGCCAACGTTCAAAAAACGTTGTTTTTTGTGACCGCACTTTTTGTCCTGCAGGAAATTTTAGTGc                               <  1:587724/251‑1 (MQ=255)                                                                                                                                                                                                                       gTCATGTTCGATTTATCTAAAAAAATGCCTGTAATGATAATATTAACTGCTTAGAAAATCACGCAATTTATCACGTTTTACGCAAATTATTCGTGAGTAGAAAAGGAt                                                                                                                                                                            <  1:736031/108‑1 (MQ=255)                                                                                                                                                                                                                       gTCATGTTCGATTTATCTAAAAAAATGCCTGTAATGATAATATTAACTGCTTAGAAAATCACGCAATTTATCACGTTTTACGCAAATTATTCGTGAGTAGAAAAGGATACCGATAAAAAACGCCCGAAAAAATTATTTACAAACTCGCTCTCTCGTTTCTTCACCCCTAACGGGCGGTTGGAAAAGCCAACGTTCAAAAAACGTTGTTTTTTGTGACCGCACTTTTTGTCCTGCAGGAAAtt                                      >  1:783889/1‑242 (MQ=255)                                                                                                                                                                                                                          aTGTTCGATTTATCTAAAAAAATGCCTGTAATGATAATATTAACTGCTTAGAAAATCACGCAATTTATCACGTTTTACGCAAATTATTCGTGAGTAGAAAAGGATACCGATAAAAAACGCCCGAAAAAATTATTTACAAACTCGCTCTCTCGTTTCTTCACCCCTAACGGGCGGTTGGAAAAGCCAACGTTCAAAAAACGTTGTTTTTTGTGACCGCACTTTTTGTCCTGCAGGAAATTTTAGTGCTGAAt                          <  1:792888/251‑1 (MQ=255)                                                                                                                                                                                                                          aTGTTCGATTTATCTAAAAAAATGCCTGTAATGATAATATTAACTGCTTAGAAAATCACGCAATTTATCACGTTTTACGCAAATTATTCGTGAGTAGAAAAGGATACCGATAAAAAACGCCCGAAAAAATTATTTACAAACTCGCTCTCTCGTTTCTTCACCCCTAACGGGCGGTTGGAAAAGCCAACGTTCAAAAAACGTTGTTTTTTGTGACCGCACTTTTTGTCCTGCAGGAAATTTTAGTGCTGAAt                          <  1:58366/251‑1 (MQ=255)                                                                                                                                                                                                                             ttCGATTTATCTAAAAAAATGCCTGTAATGATAATATTAACTGCTTAGAAAATCACGCAATTTATCACGTTTTACGCAAATTATTCGTGAGTAGAAAAGGATACCGATAAAAAACGCCCGAAAAAATTATTTACAAACTCGCTCTCTCGTTTCTTCACCCCTAACGGGCGGTTGGAAAAGCCAACGTTCAAAAAACGTTGTTTTTTGTGACCGCACTTTTTGTCCTGCAGGAAATTTTAGTGCTGAATCt                        <  1:573328/250‑1 (MQ=255)                                                                                                                                                                                                                                 aTTTATCTAAAAAAATGCCTGTAATGATAATATTAACTGCTTAGAAAATCACGCAATTTATCACGTTTTACGCAAATTATTCGTGAGTAGAAAAGGATACCGATAAAAAACGCCCGAAAAAATTATTTACAAACTCGCTCTCTCGTTTCTTCACCCCTAACGGGCGGTTGGAAAAGCCAACGTTCAAAAAACGTTGTTTTTTGTGACCGCACTTTTTGTCCTGCAGGAAATTTTAGTGCTGAATCTATTa                    <  1:370322/250‑1 (MQ=255)                                                                                                                                                                                                                                     aTCTAAAAAAATGCCTGTAATGATAATATTAACTGCTTAGAAAATCACGCAATTTATCACGTTTTACGCAAATTATTCGTGAGTAGAAAAg                                                                                                                                                                               >  1:232728/1‑91 (MQ=255)                                                                                                                                                                                                                                        tAAAAAAATGCCTGTAATGATAATATTAACTGCTTAGAAAATCACGCAATTTATCACGTTTTACGCAAATTATTCGTGAGTAGAAAAGGATACCGATAAAAAACGCCCGAAAAAATTATTTACAAACTCGCTCTCTCGTTTCTTCACCCCTAACGGGCGGTTGGAAAAGCCAACGTTCAAAAAACGTTGTTTTTTGTGACCGCACTTTTTGTCCTGCAGGAAATTTTAGTGCTGAATCTATTAAGCTAttt            <  1:789652/251‑1 (MQ=255)                                                                                                                                                                                                                                         aaaaaaaTGCCTGTAATGATAATATTAACTGCTTAGAAAATCACGCAATTTATCACGTTTTACGCAAATTATTCGTGAGTAGAAAAGGATAc                                                                                                                                                                          >  1:774718/1‑92 (MQ=255)                                                                                                                                                                                                                                         aaaaaaaTGCCTGTAATGATAATATTAACTGCTTAGAAAATCACGCAATTTATCACGTTTTACGCAAATTATTCGTGAGTAGAAAAGGATACCGATAAAAAACGCCCGAAAAAATTATTTACAAACTCGCTCTCTCGTttct                                                                                                                        >  1:178189/1‑142 (MQ=255)                                                                                                                                                                                                                                         aaaaaaaTGCCTGTAATGATAATATTAACTGCTTAGAAAATCACGCAATTTATCACGTTTTACGCAAATTATTCGTGAGTAGAAAAGGATACCGATAAAAAACGCCCGAAAAAATTATTTACAAACTCGCTCTCTCGTTTCTTCACCCCTAACGGGCGGTTGGAAAAGCCAACGTTCAAAAAACGTTGTTTTTTGTGACCGCACTTTTTGTCCTGCAGGAAATTTTAGTGCTGAATCTATTAAGCTAtttt           <  1:589363/251‑1 (MQ=255)                                                                                                                                                                                                                                          aaaaaaTGCCTGTAATGATAATATTAACTGCTTAGAAAATCACGCAATTTATCTCGTTTTACGCAAATTATTCGTGAGTAGAAAAGGATGCCGATAAAAAACGCCCGAAAAAATTATTTACAAACTCGCTCTCTCGTTTCTTCTCCCCTAACGGGCGGTTGGAAAAGCCAACGTTCAAAAAACGTTGTTTTTTGTGACCGCACTTTTTGTCCTGCAGGAAATTTTAGTGCTGAATCTATTAAGCTAttttt          <  1:372235/251‑1 (MQ=255)                                                                                                                                                                                                                                          aaaaaaTGCCTGTAATGATAATATTAACTGCTTAGAAAATCACGCAATTTATCACGTTTTACGCAAATTATTCGTGAGTAGAAAAGGATACCGATAAAAAACGCCCGAAAAAATTATTTACAAACTCGCTCTCTCGTTTCTTCACCCCTAACGGGCGGTTGGAAAAGCCAACGTTCAAAAAACGTTGTTTTTTGTGACCGCACTTTTTGTCCTGCAGGAAATTTTAGTGCTGAATCTATTAAGCTAttttt          <  1:247664/251‑1 (MQ=255)                                                                                                                                                                                                                                           aaaaaTGCCTGTAATGATAATATTAACTGCTTAGAAAATCACGCAATTTATCACGTTTTACGCAAATTATTCGTGAGTAGAAAAGGATACCGATAAAAAACGCCCGAAAAAATTATTTACAAACTCGCTCTCTCGTTTCTTCACCCCTAACGGGCGGTTGGAAAAGCCAACGTTCAAAAAACGTTGTTTTTTGTGACCGCACTTTTTGTCCTGCAGGAAATTTTAGTGCTGAATCTATTAAGCTATTTTTc         <  1:54016/251‑1 (MQ=255)                                                                                                                                                                                                                                                tGCCTGTAATGATAATATTAACTGCTTAGAAAATCACGCAATTTATCACGTTTTACGCAAATTATTCGTGAGTAGAAAAGGATACCGATAAAAAACGCCCGAAAAAATTATTTACAAACTCGCTCTCTCGTTTCTTCACCCCTAACGGGCGGTTGGAAAAGCCAACGTTCAAAAAACGTTGTTTTTTGTGACCGCACTTTTTGTc                                                  >  1:297307/1‑205 (MQ=255)                                                                                                                                                                                                                                                  ccTGTAATGATAATATTAACTGCTTAGAAAATCACGCAATTTATCACGTTTTACGCAAATTATTCGTGAGTAGAAAAGGATACCGATAAAAAACGCCCGAAAAAATTATTTACAAACTCGCTCTCTCGTTTCTTCACCCCTAACGGGCGGTTGGAAAAGCCAACGTTCAAAAAACGTTGTTTTTTGTGACCGCACTTTTTGTCCTGCAGGAAATTTTAGTGCTGAATCTATTAAGc                 <  1:726129/236‑1 (MQ=255)                                                                                                                                                                                                                                                  ccTGTAATGATAATATTAACTGCTTAGAAAATCACGCAATTTATCACGTTTTACGCAAATTATTCGTGAGTAGAAAAGGATACCGATAAAAAACGCCCGAAAAAATTATTTACAAACTCGCTCTCTCGTTTCTTCACCCCTAACGGGCGGTTGGAAAAGCCAACGTTCAAAAAACGTTGTTTTTTGTGACCGCACTTTTTGTCCTGCAGGAAATTTTAGTGCTGAATCTATTAAGCTATTTTTCATAGACa  >  1:650515/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       CTTGTTTGGCTTCGTTAATTTTTGCACCCACCGCCGGACGCTCTTCCGCCGCCACATTGCGTAGTTCTTGCATTAATTGGGTAAAATGCCCTTTTTTACC‑AAAATATTCCACCCGAATTTCATCAAGTGCCGCCAGGCTTTTATTTTCAATACTGTCTATAGCATTTCTTGCTTTCTCCACAAGATCTTTAAGATGCTGCATATATATCCTCTGTCATGTTCGATTTATCTAAAAAAATGCCTGCAATGATAATATTAACTGCTTAGAAAATCACGCAATTTATCACGTTTTACGCAAATTATTCGTGAGTAGAAAAGGATACCGATAAAAAACGCCCGAAAAAATTATTTACAAACTCGCTCTCTCGTTTCTTCACCCCTAACGGGCGGTTGGAAAAGCCAACGTTCAAAAAACGTTGTTTTTTGTGACCGCACTTTTTGTCCTGCAGGAAATTTTAGTGCTGAATCTATTAAGCTATTTTTCATAGACA  >  1/1330362‑1330852

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑