Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,329,321	T→C	intergenic (+190191/+63990)	unknown → / ← unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,329,321	0	T	C	100.0%	230.4 / NA	77	intergenic (+190191/+63990)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (39/38);  total (39/38)

TTTCAATGGCGTTATCATCTAATTTCAAATAATCACGTAAATTCGTGCCAATCGGCGCGTCTTGCAGCAATTCGATAATGCCGTTAGCGTCGGATTCAATACCGAGTTCGCTGAATGAACAGAGCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCATACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAACCCAACATGGTAATTTGATCGCAAAGTTGCTCCGTGGAAATTACCGGATTAACCCATTCACGTACCCATTGTTCACTAAATTTCATACTTATCTC  >  1/1329077‑1329563                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                   tttCAATGGCGTTATCATCTAATTTCAAATAATCACGTAAATTCGTGCCAATCGGCGCGTCTTGCAGCAATTCGATAATGCCGTTAGCGTCGGATTCAATACCGAGTTCGCTGAATGAACAGAGCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAata                                                                                                                                                                                                                                              >  1:488578/1‑251 (MQ=255)   tCAATGGCGTTATCATCTAATTTCAAATAATCACGTAAATTCGTGCCAATCGGCGCGTCTTGCAGCAATTCGATAATGCCGTTAGCGTCGGATTCAATACCGAGTTCGCTGAATGAACAGAGCATCCCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATc                                                                                                                                                                                                                                            <  1:782841/251‑1 (MQ=255)       tGGCGTTATCATCTAATTTCAAATAATCACGTAAATTCGTGCCAATCGGCGCGTCTTGCAGCAATTCGATAATGCCGTTAGCGTCGGATTCAATACCGAGTTCGCTGAATGAACAGAGCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAAt                                                                                                                                                                                                                                        <  1:752282/251‑1 (MQ=255)        ggCGTTATCATCTAATTTCAAATAATCACGTAAATTCGTGCCAATCGGCGCGTCTTGCAGCAATTCGATAATGCCGTTAGCGTCGGATTCAATACCGAGTTCGCTGAATGAACAGAGCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAAt                                                                                                                                                                                                                                        <  1:825451/250‑1 (MQ=255)         gCGTTATCATCTAATTTCAAATAATCACGTAAATTCGTGCCAATCGGCGCGTCTTGCAGCAATTCGATAATGCCGTTAGCGTCGGATTCAATACCGAGTTCGCTGAATGAACAGAGCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATaa                                                                                                                                                                                                                                      >  1:496260/1‑251 (MQ=255)          cGTTATCATCTAATTTCAAATAATCACGTAAATTCGTGCCAATCGGCGCGTCTTGCAGCAATTCGATAATGCCGTTAGCGTCGGATTCAATACCGAGTTCGCTGAATGAACAGAGCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATaa                                                                                                                                                                                                                                      <  1:663380/250‑1 (MQ=255)            ttATCATCTAATTTCAAATAATCACGTAAATTCGTGCCAATCGGCGCGTCTTGCAGCAATTCGATAATGCCGTTAGCGTCGGATTCAATACCGAGTTCGCTGAATGAACAGAGCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACg                                                                                                                                                                                                                                   <  1:207831/251‑1 (MQ=255)                 atcTAATTTCAAATAATCACGTAAATTCGTGCCAATCGGCGCGTCTTGCAGCAATTCGATAATGCCGTTAGCGTCGGATTCAATACCGAGTTCGCTGAATGAACAGAGCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTcg                                                                                                                                                                                                                               >  1:386363/1‑250 (MQ=255)                 atcTAATTTAAAATAATCACGTAAATTCGTGCCAATCGGCGCGTCTTGCAGCAATTCGATAATGCCGTTAGCGTCGGATTCAATACCGAGTTCGCTGAATGAACAGAGCATCCCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAat                                                                                                                                                                                                                                               <  1:785477/234‑1 (MQ=255)                         tCAAATAATCACGTAAATTCGTGCCAATCGGCGCGTCTTGCAGCAATTCGATAATGCCGTTAGCGTCGGATTCAATACCGAGTTCGCTGAATGAACAGAGCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCcaca                                                                                                                                                                                                                      <  1:349660/251‑1 (MQ=255)                                atCACGTAAATTCGTGCCAATCGGCGCGTCTTGCAGCAATTCGATAATGCCGTTAGCGTCGGATTCAATACCGAGTTCGCTGAATGAACAGAGCATACCTTCGGAAGGTTGCCCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACtt                                                                                                                                                                                                               <  1:394851/251‑1 (MQ=255)                                atCACGTAAATTCGTGCCAATCGGCGCGTCTTGCAGCAATTCGATAATGCCGTTAGCGTCGGATTCAATACCGAGTTCGCTGAATGAACAGAGCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACtt                                                                                                                                                                                                               <  1:689358/251‑1 (MQ=255)                                  cACGTAAATTCGTGCCAATCGGCGCGTCTTGCAGCAATTCGATAATGCCGTTAGCGTCGGATTCAATACCGAGTTCGCTGAATGAATAGAGCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTg                                                                                                                                                                                                             >  1:250229/1‑251 (MQ=255)                                  cACGTAAATTCGTGCCAATCGGCGCGTCTTGCAGCAATTCGATAATGCCGTTAGCGTCGGATTCAATACCGAGTTCGCTGAATGAACAGAGCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTg                                                                                                                                                                                                             >  1:412561/1‑251 (MQ=255)                                    cGTAAATTCGTGCCAATCGGCGCGTCTTGCAGCAATTCGATAATGCCGTTAGCGTCGGATTCAATACCGAGTTCGCTGAATGAACAGAGCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTgg                                                                                                                                                                                                            >  1:760688/1‑250 (MQ=255)                                    cGTAAATTCGTGCCAATCGGCGCGTCTTGCAGCAATTCGATAATGCCGTTAGCGTCGGATTCAATACCGAGTTCGCTGAATGAACAGAGCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGt                                                                                                                                                                                                           >  1:605050/1‑251 (MQ=255)                                                   aTCGGCGCGTCTTGCAGCAATTCGATAATGCCGTTAGCGTCGGATTCAATACCGAGTTCGCTGAATGAACAGAGCATCCCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTCCCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATc                                                                                                                                                                                            <  1:258484/251‑1 (MQ=255)                                                           gTCTTGCAGCAATTCGATAATGCCGTTAGCGTCGGATTCAATACCGAGTTCGCTGAATGAACAGAGCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCg                                                                                                                                                                                    >  1:385832/1‑251 (MQ=255)                                                           gTCTTGCAGCAATTCGATAATGCCGTTAGCGTCGGATTCAATACCGAGTTCGCTGAATGAACAGAGCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCg                                                                                                                                                                                    >  1:693895/1‑251 (MQ=255)                                                            tCTTGCAGCAATTCGATAATGCCGTTAGCGTCGGATTCAATACCGAGTTCGCTGAATGAACAGAGCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCgg                                                                                                                                                                                   >  1:482376/1‑251 (MQ=255)                                                            tCTTGCAGCAATTCGATAATGCCGTTAGCGTCGGATTCAATACCGAGTTCGCTGAATGAACAGAGCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCgg                                                                                                                                                                                   >  1:96607/1‑251 (MQ=255)                                                              ttGCAGCAATTCGATAATGCCGTTAGCGTCGGATTCAATACCGAGTTCGCTGAATGAACAGAGCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGgt                                                                                                                                                                                 >  1:5112/1‑251 (MQ=255)                                                                         cGATAATGCCGTTAGCGTCGGATTCAATACCGAGTTCGCTGAATGAACAGAGCATACCTTCGGAAGGTTGACCACGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATc                                                                                                                                                                                            <  1:779870/229‑1 (MQ=255)                                                                           aTAATGCCGTTAGCGTCGGATTCAATACCGAGTTCGCTGAATGAACAGAGCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCAtt                                                                                                                                                                     >  1:358327/1‑250 (MQ=255)                                                                           aTAATGCCGTTAGCGTCGGATTCAATACCGAGTTCGCTGAATGAACAGAGCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCAtt                                                                                                                                                                     >  1:168397/1‑250 (MQ=255)                                                                                gCCGTTAGCGTCGGATTCAATACCGAGTTCGCTGAATGAACAGAGCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAAtt                                                                                                                                                                                                >  1:505466/1‑218 (MQ=255)                                                                                    ttAGCGTCGGATTCAATACCGAGTTCGCTGAATGAACAGAGCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACtt                                                                                                                                                            <  1:303822/250‑1 (MQ=255)                                                                                              aTTCAATACCGAGTTCGCTGAATGAACAGAGCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTCCCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTacaac                                                                                                                                                  <  1:657638/250‑1 (MQ=255)                                                                                                 cAATACCGAGTTCGCTGAATGAACAGAGCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACg                                                                                                                                              >  1:323042/1‑251 (MQ=255)                                                                                                   aTACCGAGTTCGCTGAATGAACAGAGCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTacaac                                                                                                                                                  <  1:469331/245‑1 (MQ=255)                                                                                                       cGAGTTCGCTGAATGAACAGAGCATCCCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTg                                                                                                                                        <  1:203748/251‑1 (MQ=255)                                                                                                           ttCGCTGAATGAACAGAGCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGaaa                                                                                                                                     <  1:389523/250‑1 (MQ=255)                                                                                                                 gaatgaaCAGAGCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCgg                                                                                                                              >  1:657933/1‑251 (MQ=255)                                                                                                                 gaatgaaCAGAGCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCgg                                                                                                                              >  1:758767/1‑251 (MQ=255)                                                                                                                 gaatgaaCAGAGCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCgg                                                                                                                              >  1:119337/1‑251 (MQ=255)                                                                                                                   atgaaCAGAGCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACtt                                                                                                                                                                                                               <  1:852923/168‑1 (MQ=255)                                                                                                                       aCAGAGCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAAc                                                                                                                          >  1:466060/1‑249 (MQ=255)                                                                                                                          gagCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACtt                                                                                                                                                                                                               >  1:652210/1‑161 (MQ=255)                                                                                                                          gagCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTAt                                                                                                                                                                                             <  1:535466/179‑1 (MQ=255)                                                                                                                          gagCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTacaac                                                                                                                                                  >  1:724743/1‑222 (MQ=255)                                                                                                                          gagCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCtttt                                                                                                                     >  1:859613/1‑251 (MQ=255)                                                                                                                          gagCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCtttt                                                                                                                     >  1:366227/1‑251 (MQ=255)                                                                                                                          gagCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCtttt                                                                                                                     >  1:771776/1‑251 (MQ=255)                                                                                                                                 ccTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACg                                                                                                                                                                                                                                   >  1:420264/1‑134 (MQ=255)                                                                                                                                 ccTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCcac                                                                                                                 >  1:352951/1‑248 (MQ=255)                                                                                                                                    tCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGt                                                                                                            >  1:676854/1‑250 (MQ=255)                                                                                                                                               cccGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTCCCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAAc                                                                                                <  1:453527/250‑1 (MQ=255)                                                                                                                                                  gcgTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAACCCa                                                                                             >  1:371176/1‑251 (MQ=255)                                                                                                                                                    gTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATc                                                                                                                                                                                                                                            <  1:485348/106‑1 (MQ=255)                                                                                                                                                      aaTTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAACCCAACAt                                                                                         >  1:762832/1‑251 (MQ=255)                                                                                                                                                      aaTTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAACCCAACAt                                                                                         <  1:838973/251‑1 (MQ=255)                                                                                                                                                            tttttCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTCCCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAACCCAACATGGTaa                                                                                    <  1:642432/249‑1 (MQ=255)                                                                                                                                                            gTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAACCCAACATGGTAAt                                                                                   >  1:386380/1‑251 (MQ=255)                                                                                                                                                             ttttCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTCCCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAACCCAACATGGTAAtt                                                                                  <  1:564124/251‑1 (MQ=255)                                                                                                                                                                     aTTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATaa                                                                                                                                                                                                                                      >  1:710313/1‑95 (MQ=255)                                                                                                                                                                         tGAAATTGCCCGGCAATTTTGCGCCTTCTCCCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAACCCAACATGGTAATTTGATCGCAAAGt                                                                      <  1:409048/251‑1 (MQ=255)                                                                                                                                                                         tGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAACCCAACATGGTAATTTGATCGCAAAGt                                                                      <  1:834245/251‑1 (MQ=255)                                                                                                                                                                            aaTTGCCCGGCAATTTTGCCCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAACCCAACATGGTAATTTGATCGCAAAGTTGc                                                                   <  1:333834/251‑1 (MQ=255)                                                                                                                                                                              ttGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAACCCAACATGGTAATTTGATCGCAAAGTTGCTc                                                                 <  1:136265/251‑1 (MQ=255)                                                                                                                                                                                     gCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAACCCAACATGGTAATTTGATCGCAAAGTTGCTCCGTGGaa                                                          >  1:607838/1‑251 (MQ=255)                                                                                                                                                                                                ccTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCTACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAACCCAACATGGTAATTTGATCGCAAAGTTGCTCCGTGGAAATTACCGGAt                                                <  1:436983/250‑1 (MQ=255)                                                                                                                                                                                                  ttCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCTTTTCGCCGCACACAATATCCAATAAACTTTCGCCGCCCACATTTCCTTTGGTCACTCTTAATTTATCCTCATCCGGGTGTTGCGCGCATTCCACAACTTCGGCTCCCACAACGCCACCTAAATCGCCGGCACCCTTCTCCACACCGTCAACTTCCAGACCCAACGTGGTAATTTTATCGCAAAGCTTCTCCGTGGAAAGTACCGGATTaa                                             >  1:611992/1‑251 (MQ=255)                                                                                                                                                                                                  ttCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAat                                                                                                                                                                                                                                               >  1:215259/1‑57 (MQ=255)                                                                                                                                                                                                    ctcccGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCcaccac                                                                                                                                                              <  1:780115/133‑1 (MQ=255)                                                                                                                                                                                                    ctcccGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAACCCAACATGGTAATTTGATCGCAAAGTTGCTCCGTGGAAATTACCGGATTAAcc                                           <  1:356651/248‑1 (MQ=255)                                                                                                                                                                                                    cTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAACCCAACATGGTAATTTGATCGCAAAGTTGCTCCGTGGAAATTACCGGATTAAcc                                           <  1:700983/251‑1 (MQ=255)                                                                                                                                                                                                     ccccGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAACCCAACATGGTAATTTGATCGCAAAGTTGCTCCGTGGAAATTACCGGATTAAccc                                          <  1:857890/249‑1 (MQ=255)                                                                                                                                                                                                            caAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAACCCAACATGGTAATTTGATCGCAAAGTTGCTCCGTGGAAATTACCGGATTAACCCATTCACg                                   <  1:17854/251‑1 (MQ=255)                                                                                                                                                                                                              aGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAACCCAACATGGTAATTTGATCGCAAAGTTGCTCCGTGGAAATTACCGGATTAACCCATTCACGTa                                 >  1:562916/1‑251 (MQ=255)                                                                                                                                                                                                                   cTTTCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAACCCAACATGGTAATTTGATCGCAAAGTTGCTCCGTGGAAATTACCGGATTAACCCATTCACGTACCCAt                            >  1:834220/1‑251 (MQ=255)                                                                                                                                                                                                                    tttCAACCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGCCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAACCCAACATGGTAATTTGATCGCAAAGTTGCTCCGTGGAAATTACCGGATTAACCCATTCACGTACCCAtt                           >  1:158448/1‑251 (MQ=255)                                                                                                                                                                                                                         aCCCTTGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCcaccac                                                                                                                                                              <  1:641103/115‑1 (MQ=255)                                                                                                                                                                                                                             ttGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAACCCAACATGGTAATTTGATCGCAAAGTTGCTCCGTGGAAATTACCGGATTAACCCATTCACGTACCCATTGTTCACTaa                  <  1:270468/251‑1 (MQ=255)                                                                                                                                                                                                                             ttGACGGCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAACCCAACATGGTAATTTGATCGCAAAGTTGCTCCGTGGAAATTACCGGATTAACCCATTCACGTACCCATTGTTCACTaa                  <  1:9951/251‑1 (MQ=255)                                                                                                                                                                                                                                   gCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAACCCAACATGGTAATTTGATCGCAAAGTTGCTCCGTGGAAATTACCGGATTAACCCATTCACGTACCCATTGTTCACTAAATTTc             <  1:110631/250‑1 (MQ=255)                                                                                                                                                                                                                                   gCAATTCGGTGCGCCGCACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAACCCAACATGGTAATTTGATCGCAAAGTTGCTCCGTGGAAATTACCGGATTAACCCATCCACGTACCCATTGTTCACTAAATTTCa            >  1:96975/1‑251 (MQ=255)                                                                                                                                                                                                                                              cgccgcACACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAACCCAACATGGTAATTTGATCGCAAAGTTGCTCCGTGGAAATTACCGGATTAACCCATTCACGTACCCATTGTTCACTAAATTTCATACTTAtctc  <  1:36987/250‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                   TTTCAATGGCGTTATCATCTAATTTCAAATAATCACGTAAATTCGTGCCAATCGGCGCGTCTTGCAGCAATTCGATAATGCCGTTAGCGTCGGATTCAATACCGAGTTCGCTGAATGAACAGAGCATACCTTCGGAAGGTTGACCGCGTAATTTGGTTTTCTTGATTTTGAAATTGCCCGGCAATTTTGCGCCTTCTACCGCACAAGCCACTTTCAACCCTTGACGGCAATTCGGTGCGCCGCATACAATATCCAATAAACGTTCGCCGCCCACATTTACTTTGGTCACGCGTAATTTATCCGCATCCGGGTGTTGCGCGCATTCCACCACTTCGCCTACAACAACGCCAGTGAAATCGCCGGCAACCTTTTCCACACCGTCAACTTCCAAACCCAACATGGTAATTTGATCGCAAAGTTGCTCCGTGGAAATTACCGGATTAACCCATTCACGTACCCATTGTTCACTAAATTTCATACTTATCTC  >  1/1329077‑1329563

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑