Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,300,023	T→C	intergenic (+160893/+93288)	unknown → / ← unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,300,023	0	T	C	100.0%	209.9 / NA	74	intergenic (+160893/+93288)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (40/34);  total (40/34)

AAGTGGAAGGACGGCCGATACCACGTTTTTCCAATTCTTTTACCAACGCTGCTTCAGAGAAACGTGCCGGCGGTTTGGTGAAATATTGCTGCGGTTGCACTTCCGACAAGTTAAGTTTATCGTT‑AACGGAAA‑CCGCCGGTAACGCCTGATCTTCTGCAGATTTACTTTGTTGCAGTAACACTTTGGTCCAACCGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATTCCCCGCTGTTACGGTTAATGTTGTGCTGTCATATTGTGCCGCAGGCATTTGACATGCCACAAATTGACGCCAGATTAAATCATATAAACGCACCGCGTCTTTTTCCATGCCCGCTAAGTCATCCAAGCCCACATTGATATCGGACGGACGAATAGCTTCATGGGCTTCCTGCGCATTTTCTTTGCTGGAATAAAAATTCGGTTTTGCCGGCAAATAGGTTTGACCGTACTGTTTTTCAATG  >  1/1299789‑1300264                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                  aaggggagggccggccgatccctgttttttCCAATTCTTTTCCCACCGCTGCTTCAGAGAAACGTCCCGGCGGGTTGGAGAATTATTGCTGCGGTTCCCCTTCCGAAAATTTAATTTTATCGTT‑AACGAAAA‑CCGCCGGTAACGCCTGATCTTCTGCAGATTTACTTTGTTGCAGTAACACTTTGGTCCAACCGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGtt                                                                                                                                                                                                                                   <  1:836347/226‑1 (MQ=255)  aGTGGAAGGACGGCCGATACCACGTTTTTCCAATTCTTTTACCAACGCTGCTTCAGAGAAACGTGCCGGCGGGTTGGTGAAATATTGCTGCGGTTGCACTTCCGACAAGTTAAGTTTATCGTT‑AACGGAAA‑CCGCCGGTAACGCCTGATCTTCTGCAGATTTACTTTGTTGCAGTAACACTTTGGTCCAACCGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGTTa                                                                                                                                                                                                                                  >  1:646705/1‑251 (MQ=255)  aGTGGAAGGACGGCCGATACCACGTTTTTCCAATTCTTTTACCAACGCTGCTTCAGAGAAACGTGCCGGCGGGGTGGTGTAATATTTCTGCGGTTGCACTTCCGACAAGTTAAATTTATCGTT‑AACGGAAA‑TCGCCGGGAACGCCTGATCTTCTGCAGATTTACTTTGTTGCAGTAACACTTTGGTCCAACCGCCAAAAAGTAAAATGCGTCCCTTT‑TCGGTTAAAATGTAAACCCCCGCTGTTTCGGttt                                                                                                                                                                                                                                  >  1:33359/1‑250 (MQ=255)   gTGGAGGGACGGCCAATACCCGGTTTTTCAAATTCTTTTCCCACCGCTGCTTCAGAAAAACGTGCCGGGGGGTTGGTAAAATTTTGCTGCGGTTGCACTCCCGAAAATTTAAGTTTATCGTT‑AACGAAAA‑CCGCCGGTAACGCCTGATCTTCTGCAGATTTACTTTGTTGCAGTAACACTTTGGTCCAACCGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGtt                                                                                                                                                                                                                                   <  1:648229/249‑1 (MQ=255)   gTGGAAGGACGGCCGATACCACGTTTTTCCAATTCTTTTACCAACGCTGCTTCAGAGAAACGTGCCGGCGGGTTGGTGAAATATTGCTGCGGTTGCACTTCCGACAAGTTAAGTTTATCGTT‑AACGGGAA‑CCGCCGGTAACGCCTGATCTTCTGCAGATTTACTTTGTTACAGTAACACTTTGGTCCAACCGTCAAAACGTAAAAAGCG‑CCCTTTCGCGGGTAAACTGTAATCCCCCGCTGTTACGGTTaa                                                                                                                                                                                                                                 >  1:428129/1‑251 (MQ=255)   gTGGAAGGACGGCCGATACCACGTTTTTCCAATTCTTTTACCAACGCTGCTTCAGAGAAACGTGCCGGCGGGTTGGTGAAATATTGCTGCGGTTGCACTTCCGACAAGTTAAGTTTATCGTT‑AACGGAAA‑CCGCCGGTAACGCCTGATCTTCTGCAGATTTACTTTTTTGCAGTAACACTTTGGTCCAACCGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGTTaa                                                                                                                                                                                                                                 >  1:490407/1‑251 (MQ=255)       aaGGGCGGCCGATACCACTTTTTTCCAATTCTTTTACCAACGCTGCTTCAGAAAAGCGTGCCGGGGGGTTGGTGAAATATTGCGGCGGTTCCACTTCCGACAATTTAAGTTTATCGTT‑AACGAAAA‑CCGCCGGTAACGCCTGATCTTCTGCAGATTTACTTTGTTGCAGTAACACTTTGGTCCAACCGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGTTAAtgt                                                                                                                                                                                                                              <  1:775395/250‑1 (MQ=255)       aaGGACGGCCGATCCCACGTTTTTCCATTTCTTTTACCACCGCTGCTTCAGAGAACCGTGCGGGGGGGTGGGTGAAATATTGCTGCGGTTCCACTTCCGACAAGTTAAGTTTATCGTT‑AACGGAAA‑CCGCCGGTAACGCCTGATCTTCTGCAGATTTACTTTGTTGCAGTAACACTTTGGTCCAACCGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGTTAAtgt                                                                                                                                                                                                                              <  1:759272/250‑1 (MQ=255)           ccGGCCAATACCACGTTTTTCCAATTCTTTTCCCAGCGCTGCTTCAGAGAACCGTGCCGGGGGGTTGGTAAAATATTGCTGCGGTTGCACTTCCAACAAGTTAAGTTTATCGTT‑AACGGAAA‑CCGCCGGTGACGCCTGATCTTCTGCAGATTTACTTTGTTGCAGTAACACTTTGGTCCAACCGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGTTAATGTTGTGc                                                                                                                                                                                                                         <  1:76376/250‑1 (MQ=255)            cgcccgatcccccttttttCCAATTCTTTTACAACCGCTGTTTCAGAGAAACGTGCCGGCGGTTTGGTGAAATATGGCTGCGGTTGCACTCCCGACAAGTTAATTTTATCGTT‑AACGGAAA‑CCGCCGGTAACGCCTGATCTTCTGCAGATTTACTTTGTTGCAGTAACACTTTGGTCCAACCGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGTTAATGTTGTGc                                                                                                                                                                                                                         <  1:779989/236‑1 (MQ=255)              gCCGATACCACGTTTTTCCAATTCTTTTACCAACGCTGCTTCAGAGAAACGTGCCGGCGGGTTGGTGAAATATTGCTGCGGTTGCACTTCCGACAAGTTAAGTTTATCGTT‑AACGGGAA‑CCGCCGGTAACGCCTGATCTTCTGCAGATTTACTTTGTTGCAGTAACACTTTGGTCCAACCGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGTTAATGGtgtg                                                                                                                                                                                                                          >  1:816892/1‑247 (MQ=255)                  aTACCACGTTTTTCCAATTCTTTTACCAACGCTGCTTCAGAGAAACGTGCCGGCGGGTTGGTGAAATATTGCTGCGGTTGCACTTCCGACAAGTTAAGTTTATCGTT‑AACGGAAA‑CCGCCGGTAACGCCTGATCTTCTGCAGATTTTCTTTGTTGCAGTAACACTTTGGTCCAACCGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGTTAATGTTGTGCTGTCata                                                                                                                                                                                                                  >  1:181541/1‑251 (MQ=255)                  aTACCACGTTTTTCCAATTCTTTTACCAACGCTGCTTCAGAGAAACGTGCCGGCGGGTTGGTGAAATATTGCTGCGGTTGCACTTCCGACAAGTTAAGTTTATCGTT‑AACGGAAA‑CCGCCGGTAACGCCTGATCTTCTGCAGATTTACTTTGTTGCAGTAACACTTTGGTCCAACCGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGTTAAtgt                                                                                                                                                                                                                              >  1:250968/1‑239 (MQ=255)                   tACCACGTTTTTCCAATTCTTTTCCCAACGCTGCTTCAGAGAAACGTGCCGGCGGGTTGGTAAAATATTGCTGCGGTTGCCCTTCCGAAAAGTTAATTTTATCGTT‑AACGGAAA‑CCGCCGGTAACGCCTGATCTTCTGCAGATTTACTTTGTTGCAGTAACACTTTGGTCCAACCGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGTTAATGTTGTGCTGTCatat                                                                                                                                                                                                                 <  1:154264/251‑1 (MQ=255)                   ccccACTTTTTTCCATTTCTTTTACCAACGCCGCTTCGGAGAAACGTGCCGGCGGGTTGGTAAAATTTGGCTGGGGTGGCACTCCCGAAAAGTTAAGTTTATGTTT‑AAGGGAAA‑CCGCCGGTAACGCCTGATCTTCTGCAGATTTACTTTGTTGCAGTAACACTTTGGTCCAACCGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGTTAATGTTGTGCTGTCatat                                                                                                                                                                                                                 <  1:540822/249‑1 (MQ=255)                   acgcggttttttCCAATTCTTTTACCTACGCGGCTAGAGAAAACCGTCCCGGAGGTTTGGGAAAATATGGCGTAGGTGGCACTCCAGAAAAAATAAGTTT‑TTGTTAAACGAAAG‑CCGCCGGTAACGCCTGATCTTCTGCAGATTTACTTTGTTGCAGTAACACTTTGGTCCAACCGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGTTAATGTTGTGCTGTCatat                                                                                                                                                                                                                 <  1:787855/244‑1 (MQ=255)                   acccgcGTTTTTCCAATTCTTTACCCACCGCTGCTTCAGAAAAACGTGCCGGCGGGTTGGTAAAATATTGCTGCGGTTGCACTTCCGACAATTTATTTTTATCTTT‑AACGGAAA‑CCGCCGGTAACGCCTGATCTTCTGCAGATTTACTTTGTTGCAGTAACACTTTGGTCCAACCGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGTTAATGTTGTGCTGTCatat                                                                                                                                                                                                                 <  1:530549/246‑1 (MQ=255)                     ccccttttttCAAATTCTTTTCCCAACGCTGCTTCAGAGAACCGTGCCGGCGGTTTGGTAAAATATTGCTGCGTTTGCACTCCCGACAAGTTAAGTTTATCGTT‑AACGGAAA‑CCGCCGGTAACGCCTGATCTTCTGCAGATTTACTTTGTTGCAGTAACACTTTGGTCCAACCGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGTTAATGTTGTGCTGTCATATtg                                                                                                                                                                                                               <  1:596608/246‑1 (MQ=255)                     ccACTTTTTTCCAATTCTTTTACCAACGCTGCTTCAGAGAACCTTGCCGGCGGTTTGGTGAAATATTGCTGCGGTTGCTCTTCCGACAAGTTAAGTTTATCTTT‑AACGGAAA‑CCGCCGGTAACGCCTGATCTTCTGCAGATTTACTTTGTTGCAGTAACACTTTGGTCCAACCGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGTTAATGTTGTGCTGTCATATtg                                                                                                                                                                                                               <  1:387383/251‑1 (MQ=255)                     ccACGTTTTTCCAATTCTTTTCCCACCGCTGCTTCAGAGAAACGTGCCGGGGGGTTGGTAAAATATTGCGGCGGTTGCACTTCCGACAAGTTAAGTTTATCGTT‑AACGAAAA‑CCGCCGGTAACGCCTGATCTTCTGCAGATTTACTTTGTTGCAGTAACACTTTGGTCCAACCGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGTTAATGTTGTGCTGTCATATtg                                                                                                                                                                                                               <  1:666844/251‑1 (MQ=255)                        cGTTTTTCCAATTCTTTTACCAACGCTGCTTCAGAGAAACGTGCCGGCGGGTTGGTGAAATATTGCTGCGGTTGCACTTCCGACAAGTTAAGTTTATCGTT‑AACGGGAA‑ACGCCGGTAACGCCTGATCTTCTGCAGATTTTCTTTTTTGCAGTAACACTTTGGTCCAACCGTCAAAACGTAAAATGCG‑CCCTTTTGCGGTTAAACTGTAATCCCCCGCTGTTACGGTTAATGTTGTGCTGTCATATtg                                                                                                                                                                                                               >  1:592651/1‑248 (MQ=255)                                      ttttCCCACCGCTGCTTCAGAGAAACGGGCCGGGGGGTTGGTGAAATATTGCTGCGGTTGCACTTCCGACAATTTAATTTTATCGTT‑AACGGAAA‑CCGCCGGTAACGCCTGATCTTCTGCAGATTTACTTTGTTGCAGTAACACTTTGGTCCAACCGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGTTAATGTTGTGCTGTCATATTGTGCCGCAGGCATTTGAc                                                                                                                                                                                              <  1:626368/251‑1 (MQ=255)                                      ttttACCACCGCTGCTTCAGAGAAACGGGCGGGGGGGTTGGTAAAATATTGCGGCGGTTGCACTCCCGACAAGTTAATTTTATCGTT‑ACCGGAAA‑CCGCCGGTAACGCCTGATCTTCTGCAGATTTACTTTGTTGCAGTAACACTTTGGTCCAACCGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGTTAATGTTGTGCTGTCATATTGTGCCGCAGGCATTTGAc                                                                                                                                                                                              <  1:484089/251‑1 (MQ=255)                                         tACCAACGCTGCTTCAGAGAAACGTGCCGGCGGGTTGGTGAAATATTGCTGCGGTTGCACTTCCGACAAGTTAAGTTTATCGTT‑AACGGAAA‑CCGCCGGTAACGCCTGATCTTCTGCAGATTTACTTTGTTGCAGTAACACTTTGGTCCAACCGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGTTAATGTTGTGCTGTCATATTGTGCCGCAGGCATTTGACATg                                                                                                                                                                                           >  1:312310/1‑251 (MQ=255)                                         tACCAACGCTGCTTCAGAGAAACGTGCCGGCGGGTTGGTGAAATATTGCTGCGGTTGCACTTCCGACAAGTTAAGTTTATCGTT‑AACGGAAA‑CCGCCGGTAACGCCTGATCTTCTGCAGATTTACTTTGTTGCAGTAACACTTTGGTCCAACCGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGTTAATGTTGTGCTGTCATATTGTGCCGCAGGCATTTGACATg                                                                                                                                                                                           >  1:451910/1‑251 (MQ=255)                                                   gctTCAGAGAAACGTGCCGGCGGGTTGGTGAAATATTGCTGCGGTTGCACTTCCGACAAGTTAAGTTTATCGTT‑AACGGAAA‑CCGCCGGTAACGCCTGATCTTCTGCAGATTTACTTTGTTGCAGTAACACTTTGGTCCAACCGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGTTAATGTTGTGCTGTCatat                                                                                                                                                                                                                 >  1:843918/1‑219 (MQ=255)                                                           gaAACGTGCCGGCGGGTTGGTGAAATATTGCTGCGGTTGCACTTCCGACAAGTTAAGTTTATCGTT‑AACGGAAA‑CCGCCGGTAACGCCTGATCTTCTGCAGATTTACTTTGTTGCAGTAACACTTTGGTCCAACCGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGtt                                                                                                                                                                                                                                   >  1:46200/1‑193 (MQ=255)                                                                   cgggggggttGGTGAAATTTTGCTGCGTTTCCTCTTCCGAAAAGTTAATTTTATCTTT‑ACCGAAAA‑CCGCCGGTAACGCCTGATCTTCTGCAGATTTACTTTGTTGCAGTAACACTTTGGTCCAACCGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGTTAATGTTGTGCTGTCATATTGTGCCGCAGGCATTTGACATGCCACAAATTGACGCCAGATTAAATCa                                                                                                                                                                 <  1:665242/243‑1 (MQ=255)                                                                      ttaaaagagacaaaTATTGCGGGGGTTGCACTCCCGAAAATTTATTTTTATCTTT‑AGGGGAAA‑CCGCCGGTAACGCCTGATCTTCTGCAGATTTACTTTGTTGCAGTAACACTTTGGTCCAACCGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGTTAATGTTGTGCTGTCATATTGTGCCGCAGGCATTTGACATGCCACAAATTGACGCCAGATTAAATCatat                                                                                                                                                              <  1:572895/240‑1 (MQ=255)                                                                        gggttGGTGAAATATTGCTGCGGTTGCACTTCCGACAAGTTAAGTTTATCGTT‑AACGGAAA‑CCGCCGGTAACGCCTGATCTTCTGCAGATTTACTTTGTTGCAGTAACACTTTGGTCCAACCGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGTTAAtgc                                                                                                                                                                                                                              >  1:584408/4‑184 (MQ=255)                                                                        gggttGGTAAAATATTGCTGGGGTTGCTCTCCAGAAATGTTAATTTTATCTTT‑AAGGGAAA‑CCGCCGGTAACGCCTGATCTTCTGCAGATTTACTTTGTTGCAGTAACACTTTGGTCCAACCGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGTTAATGTTGTGCTGTCATATTGTGCCGCAGGCATTTGACATGCCACAAATTGACGCCAGATTAAATCAtata                                                                                                                                                             <  1:302177/247‑1 (MQ=255)                                                                           ttGGTAAAATATTGCTGCGGTTGCCCTTCCGACAAGTTAAGTTTATCGTT‑AACGGAAA‑CCGCCGGTAACGCCTGATCTTCTGCAGATTTACTTTGTTGCAGTAACACTTTGGTCCAACCGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGTTAATGTTGTGCTGTCATATTGTGCCGCAGGCATTTGACATGCCACAAATTGACGCCAGATTAAATCATATAAAc                                                                                                                                                          <  1:34827/250‑1 (MQ=255)                                                                            tGGTAAAATTTTGCTGCGATTCCTTTTCCGAAAAGTTATTTTTTTCGTT‑AGCGAAAA‑CCGCCGGTAACGCCTGATCTTCTGCAGATTTACTTTGTTGCAGTAACACTTTGGTCCAACCGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGTTAATGTTGTGCTGTCATATTGTGCCGCAGGCATTTGACATGCCACAAATTGACGCCAGATTAAATCATATAAACGc                                                                                                                                                        <  1:802422/251‑1 (MQ=255)                                                                              gTGAAATATTGCTGCGGTTGCACTTCCGACAAGTTAAGTTTATCGTT‑AACGGAAA‑CCGCCGGTAACGCCTGATCTTCTGCAGATTTACTTTGTTGCAGTAACACTTTGGTCCAACCGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACgg                                                                                                                                                                                                                                     >  1:233449/1‑172 (MQ=255)                                                                              gTGAAATATTGCTGCGGTTGCACTTCCGACAAGTTAAGTTTATCGTT‑AACGGAAA‑CCGCCGGTAACGCCTGATCTTCTGCAGATTTACTTTGTTGCAGTAACACTTTGGTCCAACCGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGTTAATGTTGTGCTGTCATATTGTGCCGCAGGCATTTGACATGCCACAAATTGACGCCAGATTAAATCATATAAACGCa                                                                                                                                                       >  1:259090/1‑250 (MQ=255)                                                                               taaaaTTTTGCTGCGGTTGCACTTCCAACAATTTAATTTTATCGTT‑AGCGAAAA‑CCGCCGGTAACGCCTGATCTTCTGCAGATTTACTTTGTTGCAGTAACACTTTGGTCCAACCGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGTTAATGTTGTGCTGTCATATTGTGCCGCAGGCATTTGACATGCCACAAATTGACGCCAGATTAAATCATATAAACGCAc                                                                                                                                                      <  1:760443/248‑1 (MQ=255)                                                                                   atatTGCTGCGGTTGCACTTCCGACAAGTTAAGTTTATCGTT‑AACGGAAA‑CCGCCGGTAACGCCTGATCTTCTGCAGATTTACTTTGTTGCAGTAACACTTTGGTCCAACCGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGTTAATGTTGTGCTGTCATATTGTGCCGCAGGCATTTGAc                                                                                                                                                                                              >  1:525114/1‑206 (MQ=255)                                                                                   atatTGCTGCGGTTGCACTTCCGACAAGTTAAGTTTATCGTT‑AACGGAAA‑CCGCCGGTAACGCCTGATCTTCTGCAGATTTACTTTGTTGCAGTAACACTTTGGTCCAACCGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGTTAATGTTGTGCTGTCATATTGTGCCGCAGGCATTTGACATGCCACAAATTGACGCCAGATTAAATCATATAAACGCACCGCGt                                                                                                                                                 >  1:796108/1‑251 (MQ=255)                                                                                     atTGCTGCGGTTGCACTTCCGACAAGTTAAGTTTATCGTT‑AACGGAAA‑CCGCCGGTAACGCCTGATCTTCTGCAGATTTACTTTGTTGCAGTAACACTTTGGTCCAACCGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGTTAATGTTGTGCTGTCATATTGTGCCGCAGGCATTTGACATGCCACAAATTGACGCCAGATTAAATCATATaaa                                                                                                                                                           >  1:613293/1‑239 (MQ=255)                                                                                            cggtcgcctttccaaaaatttaatttTATCTTT‑AA‑GGAAACCCGCCGGTAACGCCTGATCTTCTGCAGATTTACTTTGTTGCAGTAACACTTTGGTCCAACCGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGTTAATGTTGTGCTGTCATATTGTGCCGCAGGCATTTGACATGCCACAAATTGACGCCAGATTAAATCATATAAACGCACCGCGTCTTTTTcc                                                                                                                                         <  1:488973/231‑1 (MQ=255)                                                                                                  cACTTCCGACAAGTTAAGTTTATCGTT‑AACGGAAA‑CCGCCGGTAACGCCTGATCTTCTGCAGATTTACTTTGTTGCAGTAACACTTTGGTCCAACCGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGTTAATGTTGTGCTGTCATATTGTGCCGCAGGCATTTGACATGCCACAAATTGACGCCAGATTAAATCATATAAACGCACCGCGTCTTTTTCCATGCCCg                                                                                                                                  >  1:113033/1‑251 (MQ=255)                                                                                                      tCCGACAAGTTAAGTTTATCGTT‑AACGGAAA‑CCGCCGGTAACGCCTGATCTTCTGCAGATTTACTTTGTTGCAGTAACACTTTGGTCCAACCGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGTTAATGTTGTGCTGTCATATTGTGCCGCAGGCATTTGACATGCCACAAATTGACGCCAGATTAAATCATATAAACGCACCGCGTCTTTTTCCATGCCCGCTaa                                                                                                                              >  1:407815/1‑251 (MQ=255)                                                                                                      tCCGACAAGTTAAGTTTATCGTT‑AACGGAAA‑CCGCCGGTAACGCCTGATCTTCTGCAGATTTACTTTGTTGCAGTAACACTTTGGTCCAACCGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGTTAATGTTGTGCTGTCATATTGTGCCGCAGGCATTTGACATGCCACAAATTGACGCCAGATTAAATCATATAAACGCACCGCGTCTTTTTCCATGCCCGCTaa                                                                                                                              >  1:756859/1‑251 (MQ=255)                                                                                                       ccgaaaagtaagttttATCTTA‑AACGAAAA‑CCGCCGGTAACGCCTGATCTTCTGCAGATTTACTTTGTTGCAGTAACACTTTGGTCCAACCGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGTTAATGTTGTGCTGTCATATTGTGCCGCAGGCATTTGACATGCCACAAATTGACGCCAGATTAAATCATATAAACGCACCGCGTCTTTTTCCATGCCCGCTAAg                                                                                                                             <  1:600407/238‑1 (MQ=255)                                                                                                         gACAAGTTAAGTTTATCGTT‑AACGGAAA‑CCGCCGGTAACGCCTGATCTTCTGCAGATTTACTTTGTTGCAGTAACACTTTGGTCCAACCGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGTTAATGTTGTGCTGTCATATtg                                                                                                                                                                                                               >  1:597511/1‑167 (MQ=255)                                                                                                             agttaagttTATCGTT‑AACGGAAA‑CCGCCGGTAACGCCTGATCTTCTGCAGATTTACTTTGTTGCAGTAACACTTTGGTCCAACCGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGTTAATGTTGTGCTGTCATATTGTGCCGCAGGCATTTGGCATGCCACAAATTGACGCCAGATTAAATCATATAAACGCACCGCGTCTTTTTCCATGCCCGCTAAGTCATc                                                                                                                        >  1:199894/1‑250 (MQ=255)                                                                                                              gttaagttTATCGTT‑AACGGAAA‑CCGCCGGTAACGCCTGATCTTCTGCAGATTTACTTTGTTGCAGTAACACTTTGGTCCAACCGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGTTAATGTTGTGCTGTCATATTGTGCCGCAGGCATTTGACATGCCACAAATTGACGCCAGATTAAATCATATAAAc                                                                                                                                                          >  1:83518/1‑215 (MQ=255)                                                                                                                tatttttATCGTT‑AACGAAAA‑CCGCCGGTAACGCCTGATCTTCTGCAGATTTACTTTGTTGCAGTAACACTTTGGTCCAACCGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGTTAATGTTGTGCTGTCATATTGTGCCGCAGGCATTTGACATGCCACAAATTGACGCCAGATTAAATCATATAAACGCACCGCGTCTTTTTCCATGCCCGCTAAGTCATCCAAg                                                                                                                    <  1:490525/247‑1 (MQ=255)                                                                                                                 aagttTATCGTT‑AACGGAAA‑CCGCCGGTAACGCCTGATCTTCTGCAGATTTACTTTGTTGCAGTAACACTTTGGTCCAACCGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGTTAATGTTGTGCTGTCATATTGTGCCGCAGGCATTTGAc                                                                                                                                                                                              >  1:86881/1‑176 (MQ=255)                                                                                                                    ttttatcgtaagggaaaa‑CCGCCGGTAACGCCTGATCTTCTGCAGATTTACTTTGTTGCAGTAACACTTTGGTCCAACCGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGTTAATGTTGTGCTGTCATATTGTGCCGCAGGCATTTGACATGCCACAAATTGACGCCAGATTAAATCATATAAACGCACCGCGTCTTTTTCCATGCCCGCTAAGTCATCCAAGcc                                                                                                                  <  1:862608/235‑1 (MQ=255)                                                                                                                        ttctttacggaaaa‑CCGCCGGTAACGCCTGATCTTCTGCAGATTTACTTTGTTGCAGTAACACTTTGGTCCAACCGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGTTAATGTTGTGCTGTCATATTGTGCCGCAGGCATTTGACATGCCACAAATTGACGCCAGATTAAATCATATAAACGCACCGCGTCTTTTTCCATGCCCGCTAAGTCATCCAAGCCCACAt                                                                                                             <  1:878868/240‑1 (MQ=255)                                                                                                                              accgaaaa‑CCGCCGGTAACGCCTGATCTTCTGCAGATTTACTTTGTTGCAGTAACACTTTGGTCCAACCGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGTTAATGTTGTGCTGTCATATTGTGCCGCAGGCATTTGACATGCCACAAATTGACGCCAGATTAAATCATATAAACGCACCGCGTCTTTTTCCATGCCCGCTAAGTCATCCAAGCCCACATTGatat                                                                                                       <  1:371503/246‑1 (MQ=255)                                                                                                                                    aacccgccgGTAACGCCTGATCTTCTGCAGATTTACTTTGTTGCAGTAACACTTTGGTCCAACCGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGTTAATGTTGTGCTGTCATATTGTGCCGCAGGCATTTGACATGCCACAAATTGACGCCAGATTAAATCATATAAACGCACCGCGTCTTTTTCCATGCCCGCTAAGTCATCCAAGCCCACATTGATATcggac                                                                                                  <  1:613525/248‑1 (MQ=255)                                                                                                                                           cgGTAACGCCTGATCTTCTGCAGATTTACTTTGTTGCAGTAACACTTTGGTCCAACCGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGTTAATGTTGTGCTGTCATATTGTGCCGCAGGCATTTGACATGCCACAAATTGACGCCAGATTAAATCATATAAACGCACCGCGTCTTTTTCCATGCCCGCTAAGTc                                                                                                                           >  1:769813/1‑219 (MQ=255)                                                                                                                                                 cGCCTGATCTTCTGCAGATTTACTTTGTTGCAGTAACACTTTGGTCCAACCGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGTTAATGTTGTGCTGTCATATTGTGCCGCAGGCATTTGACATGCCACAAATTGACGCCAGATTAAATCATATAAACGCACCGCGTCTTTTTc                                                                                                                                          >  1:395804/1‑198 (MQ=255)                                                                                                                                                 cGCCTGATCTTCTGCAGATTTACTTTGTTGCAGTAACACTTTGGTCCAACCGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGTTAATGTTGTGCTGTCATATTGTGCCGCAGGCATTTGACATGCCACAAATTGACGCCAGATTAAATCATATAAACGCACCGCGTCTTTTTCCATGCCCGCTAAGt                                                                                                                            >  1:739732/1‑212 (MQ=255)                                                                                                                                                 cGCCTGATCTTCTGCAGATTTACTTTGTTGCAGTAACACTTTGGTCCAACCGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGTTAATGTTGTGCTGTCATATTGTGCCGCAGGCATTTGACATGCCACAAATTGACGCCAGATTAAATCATATAAACGCACCGCGTCTTTTTCCATGCCCGCTAAGTCATCCAAGCCCACATTGATATCGGACGGACGAATAGCtt                                                                                     <  1:598304/251‑1 (MQ=255)                                                                                                                                                         cttctGCAGATTTACTTTGTTGCAGTAACACTTTGGTCCAACCGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGTTAATGTTGTGCTGTCATATTGTGCCGCAGGCATTTGACATGCCACAAATTGACGCCAGATTAAATCATATAAAc                                                                                                                                                          >  1:538891/1‑174 (MQ=255)                                                                                                                                                            ctGCAGATTTACTTTGTTGCAGTAACACTTTGGTCCAACCGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGTTAATGTTGTGCTGTCATATTGTGCCGCAGGCATTTGACATGCCACAAATTGACGCCAGATTAAATCATATAAACGCACCGCGTCTTTTTCCATGCCCGCTAAGTCATCCAAGCCCACATTGATATCGGACGGACGACTAGCTTCATGGGCTTc                                                                           >  1:875131/1‑250 (MQ=255)                                                                                                                                                            ctGCAGATTTACTTTGTTGCAGTAACACTTTGGTCCAACCGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGTTAATGTTGTGCTGTCATATTGTGCCGCAGGCATTTGACATGCCACAAATTGACGCCAGATTAAATCATATAAACGCACCGCGTCTTTTTCCATGCCCGCTAAGTCATCCAAGCCCACATTGATATCGGACGGACGAATAGCTTCATGGGCTTcc                                                                          <  1:123618/251‑1 (MQ=255)                                                                                                                                                                 gATTTACTTTGTTGCAGTAACACTTTGGTCCAACCGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGTTAATGTTGTGCTGTCa                                                                                                                                                                                                                    <  1:828809/108‑1 (MQ=255)                                                                                                                                                                 gATTTACTTTGTTGCAGTAACACTTTGGTCCAACCGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGTTAATGTTGTGCTGTCATATTGTGCCGCAGGCATTTGACATGCCACAAATTGACGCCAGATTAAATCATATAAACGCACCGCGTCTTTTTCCATGCCCGCTAAGTCATCCAAGCCCACATTGATATCGGACGGACGAATAGCTTCATGGGCTTCCTgcg                                                                      >  1:147404/1‑250 (MQ=255)                                                                                                                                                                  aTTTCCTTTGTTGCAGTAACACTTTGGCCCAACCGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGTTAATGTTGTGCTGTCCTATTGTGCCGCAGGCATTTGACATGCCACAAATTGACGCCAGATTAAATCATATAAACGCCCCGCGTCTTTTTCCATGCCCGCTAAGTCATCCAAGCCCACATTGATATCGGACGGACGAATAGCTTCATGGGCTTCCTgcgc                                                                     <  1:405556/250‑1 (MQ=255)                                                                                                                                                                                aGTAACACTTTGGTCCAACCGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGTTAATGTTGTGCTGTCATATTGTGCCGCAGGCATTTGACATGCCACAAATTGACGCCAGATTAAATCATATAAACGCACcg                                                                                                                                                    >  1:134615/1‑157 (MQ=255)                                                                                                                                                                                    acacTTTGGTCCAACCGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGTTAATGTTGTGCTGTCATATTGTGCCGCAGGCATTTGACATGCCACAAATTGACGCCAGATTAAATCATATAAACGCACCGCGTCTTTTTCCATGCCCGCTAAGTCATCCAAGCCCACATTGATATCGGACGGACGAATAGCTTCATGGGCTTCCTGCGCATTTTCTTTGCTGGAATaa                                                  >  1:565325/1‑251 (MQ=255)                                                                                                                                                                                     cacTTTGGTCCAACCGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGTTAATGTTGTGCTGTCATATTGTGCCGCAGGCATTTGACATGCCACAAATTGACGCCAGATTAAATCATATAAACGCACCGCGTCTTTTTCCATGCCCGCTAAGTCATCCAAGCCCACATTGATATCGGACGGACGAATAGCTTCATGGGCTTCCTGCGCATTTTCTTTGCTGGAATaaa                                                 >  1:583802/1‑251 (MQ=255)                                                                                                                                                                                                 aCCGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGTTAATGTTGTGCTGTCATATTGTGCCGCAGGCATTTGACATGCCACAAATTGACGCCAGATTAAATCATATAAACGCACCGCGTCTTTTTCCATGCCCGCTAAGTCATCCAAGCCCACATTGatat                                                                                                       <  1:55888/185‑1 (MQ=255)                                                                                                                                                                                                  ccGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGTTAATGTTGTGCTGTCATATTGTGCCGCAGGCATTTGACATGCCACAAATTGACGCCAGATTAAATCATATAAACGCACCGCGTCTTTTTCCATGCCCGCTAAGTCATCCAAGCCCACAt                                                                                                             >  1:669550/1‑178 (MQ=255)                                                                                                                                                                                                     tCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGTTAATGTTGTGCTGTCATATTGTGCCGCAGGCATTTGACATGCCACAAATTGACGCCAGATTAAATCATATAAACGCACCGCGTCTTTTTcc                                                                                                                                         >  1:190793/1‑147 (MQ=255)                                                                                                                                                                                                           cGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGTTAATGTTGTGCTGTCATATTGTGCCGCAGGCATTTGACATGCCACAAATTGACGCCAGATTAAATCATATAAACGCACCGCGTCTTTTTCCATGCCCGCTAAGTCATCCAAGCCCACATTGATATCGGACGGACGAATAGCTTCATGGGCTTCCTGCGCATTTTCTTTGCTGGAATAAAAATTCGGTTTTGCCGGCAAATa                           >  1:359010/1‑251 (MQ=255)                                                                                                                                                                                                                aaTGCG‑CCCTTTCGCGGTTAAACTGTAATCCCCCGCTGTTACGGTTAATGTTGTGCTGTCATATTGTGCCGCAGGCATTTGAc                                                                                                                                                                                              >  1:778866/1‑83 (MQ=255)                                                                                                                                                                                                                                 gTTAAACTGTAATCCCCCGCTGTTACGGTTAATGTTGTGCTGTCATATTGTGCCGCAGGCATTTGACATGCCACAAAt                                                                                                                                                                                   <  1:195926/78‑1 (MQ=255)                                                                                                                                                                                                                                     aaCTGTAATCCCCCGCTGTTACGGTTAATGTTGTGCTGTCATATTGTGCCGCAGGCATTTGACATGCCACAAATTGACGCCAGATTAAATCATATAAACGCACCGCGTCTTTTTCCATGCCCGCTAAGTCATCCAAGCCCACATTGATATCGGACGGACGAATAGCTTCATGGGCTTCCTGCGCATTTTCTTTGCTGGAATAAAAATTCGGTTTTGCCGGCAAATAGGTTTGACCGTACTGTTTTTCAATg  >  1:165272/1‑251 (MQ=255)                                                                                                                                                                                                                                         gTAATCCCCCGCTGTTACGGTTAATGTTGTGCTGTCATATTGTGCCGCAGGCATTTGACATGCCACAAATTGACGCCAGATTAAATCATATAAACGCACCGCGTCTTTTTCCATGCCCGCTAAGTCATCCAAGCCCACATTGATATCGGACGGACGAATAGCTTCATGGGCTTCCTGCGCATTTTCTTTGCTGGAATAAAAATTCGGTTTTGCCGGCAAATAgg                         >  1:246157/1‑224 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                  AAGTGGAAGGACGGCCGATACCACGTTTTTCCAATTCTTTTACCAACGCTGCTTCAGAGAAACGTGCCGGCGGTTTGGTGAAATATTGCTGCGGTTGCACTTCCGACAAGTTAAGTTTATCGTT‑AACGGAAA‑CCGCCGGTAACGCCTGATCTTCTGCAGATTTACTTTGTTGCAGTAACACTTTGGTCCAACCGTCAAAACGTAAAATGCG‑CCCTTTCGCGGTTAAACTGTAATTCCCCGCTGTTACGGTTAATGTTGTGCTGTCATATTGTGCCGCAGGCATTTGACATGCCACAAATTGACGCCAGATTAAATCATATAAACGCACCGCGTCTTTTTCCATGCCCGCTAAGTCATCCAAGCCCACATTGATATCGGACGGACGAATAGCTTCATGGGCTTCCTGCGCATTTTCTTTGCTGGAATAAAAATTCGGTTTTGCCGGCAAATAGGTTTGACCGTACTGTTTTTCAATG  >  1/1299789‑1300264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑