Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,295,399	C→T	intergenic (+156269/+97912)	unknown → / ← unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,295,399	0	C	T	100.0%	195.4 / NA	68	intergenic (+156269/+97912)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (43/25);  total (43/25)

CATTGGCTGTCATTACTGCAATTGATGAACCGCCACACCAATTCGCTTCGTTAAAACAAGTACTTGAATTAACATACGAACATGAAAAATTGATTACTGCCAAAATCAATGAATTGGTTGGTAGAACATTTGAAGAGAAGGACTACTCTGCTTTTAACTTCTTACAATGGTACGTTGAAGAACAGCACGAAGAAGAAAAATTATTCAGCGGAATTTTGGATAAACTGAATCTTCTTGGCGAAGATGGAAAAGGTTTATTCCTTGTTGATAAAGATTTAGGCGCTTTAGCAGAAAGCAACTAGTCCGGTTTAAATAAAAGGCTAAGTCACATTGATTTAGCCTTTCTTTTTCTCATTCCAAAACTTAATTTTTATTCTCAGAAAGTGCGGTGTTTTTTTCAAGTGTTTTTGCCGGTGGTTGACCTGTTCCACTGGCTTCATAATAAAACGTGCGATAAAAAAAT  >  1/1295175‑1295637                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                               cATTGGCTGTCATTACTGCAATTGATGAACCGCCACACCAATTCGCTTCGTTAAAACAAGTACTTGAATTAACATACGAACATGAAAAATTGATTACTGCCAAAATCAATGAATTGGTTGGTAGAACATTTGAAGAGAAGGACTACTCTGCTTTTAACTTCTTACAATGATACGTTGAAGAACAGCACGAAGAAGAAAAATTATTCAGCGGAATTTTGGATAAATTGAATCTTCTTGGCGAAGATGGaaaa                                                                                                                                                                                                                      >  1:431927/1‑251 (MQ=255)   ttgccTGTCATTACTGAAATTGATGAACCGCCACACCAATTCGCTTCGTTAAAACAAGTACTTGAATTAACATACGAACATGAAAAATTGATTACTGCCAAAATCAATGAATTGGTTGGTAGAACATTTGAAGAGAAGGACTACTCTGCTTTTAACTTCTTACAATGGTACGTTGAAGAACAGCACGAAGAAGAAAAATTATTCAGCGGAATTTTGGATAAATTGAATCTTCTTGGCGAAGATGGAAAAgg                                                                                                                                                                                                                    <  1:126791/247‑1 (MQ=255)          tCATTACTGCAATTGATGAACCGCCACACCAATTCGCTTCGTTAAAACAAGTACTTGAATTAACATACGAACATGAAAAATTGATTACTGCCAAAATCAATGAATTGGTTGGTAGAACATTTGAAGAGAAGGACTACTCTGCTTTTAACTTCTTACAATGGTACGTTGAAGAACAGCACGAAGAAGAAAAATTATTCAGCGGAATTTTGGATAAATTGAATCTTCTTGGCGAAGATGGAAAAGGTTTATTc                                                                                                                                                                                                             <  1:818486/251‑1 (MQ=255)          tCATTACTGAAATTGATGAACCGCCACACCAATTCGCTTCGTTAAAACAAGTACTTGAATTAACATACGAACATGAAAAATGGATTACTGCCAAAATCAATGAATTGGTTGGTAGAACATTTGAAGAGAAGGACTACTCTGCTTTTAACTTCTTACAATGGTACGTTGAAGAACAGCACGAAGAAGAAAAATTATTCAGCGGAATTTTGGATAAATTGAATCTTCTTGGCGAAGATGGAAAAGGTTTATTc                                                                                                                                                                                                             <  1:452220/251‑1 (MQ=255)             ttACTGCAATTGATGAACCGCCACACCAATTCGCTTCGTTAAAACAAGTACTTGAATTAACATACGAACATGAAAAATTGATTACTGCCAAAATCAATGAATTGGTTGGTAGAACATTTGAAGAGAAGGACTACTCTGCTTTTAACTTCTTACAATGGTACGTTGAAGAACAGCACGAAGAAGAAAAATTATTCAGCGGAATTTTGGATAAATTGAATCTTCTTGGCGAAGATGGAAAAGGTTTATTCCtt                                                                                                                                                                                                          >  1:472796/1‑251 (MQ=255)                       tgatgaACCGCCACACCAATTCGCTTCGTTAAAACAAGTACTTGAATTAACATACGAACATGAAAAATTGATTACTGCCAAAATCAATGAATTGGTTGGTAGAACATTTGAAGAGAAGGACTACTCTGCTTTTAACTTCTTACAATGGTACGTTGAAGAACAGCACGAAGAAGAAAAATTATTCAGCGGAATTTTGGATAAATTGAATCTTCTTGGCGAAGATGGAAAAGGTTTATTCCTTGTTGATAAAg                                                                                                                                                                                                <  1:810329/251‑1 (MQ=255)                        gatgaACCGCCACACCAATTCGCTTCGTTAAAACAAGTACTTGAATTAACATACGAACATGAAAAATTGATTACTGCCAAAATCAATGAATTGGTTGGTAGAACATTTGAAGAGAAGGACTACTCTGCTTTTAACTTCTTACAATGGTACGTTGAAGAACAGCACGAAGAAGAAAAATTATTCAGCGGAATTTTGGATAAATTGAATCTTCTTGGCGAAGATGGAAAAGGTTTATTCCTTGTTGATAAAGa                                                                                                                                                                                               >  1:102757/1‑251 (MQ=255)                                    cacCAATTCGCTTCGTTAAAACAAGTACTTGAATTAACATACGAACATGAAAAATTGATTACTGCCAAAATCAATGAATTGGTTGGTAGAACATTTGAAGAGAAGGACTACTCTGCTTTTAACTTCTTACAATGGTACGTTGAAGAACAGCACGAAGAAGAAAAATTATTCAGCGGAATTTTGGATAAATTGAATCTTCTTGGCGAAGATGGAAAAGGTTTATTcc                                                                                                                                                                                                            <  1:424766/226‑1 (MQ=255)                                                            gTACTTGAATTAACATACGAACATGAAAAATTGATTACTGCCAAAATCAATGAATTGGTTGGTAGAACATTTGAAGAGAAGGACTACTCTGCTTTTAACTTCTTACAATGGTACGTTGAAGAACAGCACGAAGAAGAAAAATTATTCAGCGGAATTTTGGATAAATTGACTCTTCTTGGCGAAGATGGAAAAGGTTTATTCCTTGTTGATAAAGATTTAGGCGCTTTAGCAGAAAGCAACTAGTCCGGttt                                                                                                                                                           >  1:426406/1‑251 (MQ=255)                                                            gTACTTGAATTAACATACGAACATGAAAAATTGATTACTGCCAAAATCAATGAATTGGTTGGTAGAACATTTGAAGAGAAGGACTACTCTGCTTTTAACTTCTTACAATGGTACGTTGAAGAACAGCACGAAGAAGAAAAATTATTCAGCGGAATTTTGGATAAATTGAATCTTCTTGGCGAAGATGGAAAAGGTTTATTCCTTGTTGATAAAGATTTAGGCGCTTTAGCAGAAAGCAACTAGTCCGGttt                                                                                                                                                           >  1:83611/1‑251 (MQ=255)                                                                ttGAATTAACATACGAACATGAAAAATTGATTACTGCCAAAATCAATGAATTGGTTGGTAGAACATTTGAAGAGAAGGACTACTCTGCTTTTAACTTCTTACAATGGTACGTTGAAGAACAGCACGAAGAAGAAAAATTATTCAGCGGAATTTTGGATAAATTGAATCTTCTTGGCGAAGATGGAAAAGGTTTATTCCTTGTTGATAAAGATTTAGGCGCTTTAGCAGAAAGCAACTAGTCCGGTTtaaat                                                                                                                                                       <  1:62916/251‑1 (MQ=255)                                                                       aaCATACGAACATGAAAAATTGATTACTGCCAAAATCAATGAATTGGTTGGTAGAACATTTGAAGAGAAGGACTACTCTGCTTTTAACTTCTTACAATGGTACGTTGAAGAACAGCACGAAGAAGAAAAATTATTCAGCGGAATTTTGGATAAATTGAATCTTCTTGGCGAAGATGGAAAAGGTTTATTc                                                                                                                                                                                                             >  1:630959/1‑190 (MQ=255)                                                                         cATACGAACATGAAAAATTGATTACTGCCAAAATCAATGAATTGGTTGGTAGAACATTTGAAGAGAAGGACTACTCTGCTTTTAACTTCTTACAATGGTACGTTGAAGAACAGCACGAAGAAGAAAAATTATTCAGCGGAATTTTGGATAAATTGAATCTTCTTGGCGAAGATGGAAAAGGTTTATTCCTTGTTGATAAAGATTTAGGCGCTTTAGCAGAAAGCAACTAGTCCGGTTTAAATAAAAGGCTa                                                                                                                                              >  1:800420/1‑251 (MQ=255)                                                                            aCGAACATGAAAAATTGATTACTGCCAAAATCAATGAATTGGTTGGTAGAACATTTGAAGAGAAGGACTACTCTGCTTTTAACTTCTTACAATGGTACGTTGAAGAACAGCACGAAGAAGAAAAATTATTCAGCGGAATTTTGGATAAATTGAATCTTCTTGGCGAAGATGGAAAAGGTTTATTCCTTGTTGATAAAGATTTAGGCGCTTTAGCAGAAAGCAACTAGTCCGGTTTAAATAAAAGGCTAAGt                                                                                                                                           <  1:765711/251‑1 (MQ=255)                                                                                 cATGAAAAATTGATTACTGCCAAAATCAATGAATTGGTTGGTAGAACATTTGAAGAGAAGGACTACTCTGCTTTTAACTTCTTACAATGGTACGTTGAAGAACAGCACGAAGAAGAAAAATTATTCAGCGGAATTTTGGATAAATTGAATCTTCTTGGCGAAGATGGAAAAGGTTTATTCCTTGTTGATAAAGATTTAGGCGCTTTAGCAGAAAGCAACTAGTCCGGTTTAAATAAAAGGCTAAGTCACAt                                                                                                                                      >  1:324304/1‑251 (MQ=255)                                                                                 cATGAAAAATTGATTACTGCCAAAATCAATGAATTGGTTGGTAGAACATTTGAAGAGAAGGACTACTCTGCTTTTAACTTCTTACAATGGTACGTTGAAGAACAGCACGAAGAAGAAAAATTATTCAGCGGAATTTTGGATAAATTGAATCTTCTTGGCGAAGATGGAAAAGGTTTATTCCTTGTTGATAAAGATTTAGGCGCTTTAGCAGAAAGCAACTAGTCCGGTTTAAATAAAAGGCTAAGTCACAt                                                                                                                                      >  1:767376/1‑251 (MQ=255)                                                                                 cATGAAAAATTGATTACTGCCAAAATCAATGAATTGGTTGGTAGAACATTTGAAGAGAAGGACTACTCTGCTATTAACTTCTTACAATGGTACGTTGAAGAACAGCACGAAGAAGAAAAATTATTCAGCGGAATTTTGGATAAATTGAATCTTCTTGGCGAAGATGGAAAAGGTTTATTCCTTGTTGATAAAGAt                                                                                                                                                                                              >  1:684380/1‑195 (MQ=255)                                                                                       aaaTTGATTACTGCCAAAATCAATGAATTGGTTGGTAGAACATTTGAAGAGAAGGACTACTCTGCTTTTAACTTCTTACAATGGTACGTTGAAGAACAGCACGAAGAAGAAAAATTATTCAGCGGAATTTTGGATAAATTGAATCTTCTTGGCGAAGATGGAAAAGGTTTATTCCTTGTTGATAAAGAt                                                                                                                                                                                              >  1:265647/1‑189 (MQ=255)                                                                                       aaaTTGATTACTGCCAAAATCAATGAATTGGTTGGTAGAACATTTGAAGAGAAGGACTACTCTGCTTTTAACTTCTTACAATGGTACGTTGAAGAACAGCACGAAGAAGAAAAATTATTCAGCGGAATTTTGGATAAATTGAATCTTCTTGGCGAAGATGGAAAAGGTTTATTCCTTGTTGATAAAGATTTAGGCGCTTTAGCAGAAAGCAACTAGTCCGGTTTAAATAAAAGGCTAAGTCACATTGAttt                                                                                                                                <  1:460642/251‑1 (MQ=255)                                                                                         aTTGATTACTGCCAAAATCAATGAATTGGTTGGTAGAACATTTGAAGAGAAGGACTACTCTGCTTTTAACTTCTTACAATGGTACGTTGAAGAACAGCACGAAGAAGAAAAATTATTCAGCGGAATTTTGGATAAATTGAATCTTCTTGGCGAAGATGGAAAAGGTTTATTCCTTGTTGATAAAGATTTAGGCGCTTTAGCAGAAAGCAACTAGTCCGGTTTAAATAAAAGGCTAAGTCACATTGATTTAg                                                                                                                              >  1:224036/1‑251 (MQ=255)                                                                                           tGATTACTGCCAAAATCAATGAATTGGTTGGTAGAACATTTGAAGAGAAGGACTACTCTGCTTTTAACTTCTTACAATGGTACGTTGAAGAACAGCACGAAGAAGAAAAATTATTCAGCGGAATTTTGGATAAATTGAATCTTCTTGGCGAAGATGGAAAAGGTTTATTCCTTGTTGATAAAGATTTAGGCGCTTTAGCAGAAAGCAACTAGTCCGGTTTAAATAAAAGGCTAAGTCACATTGATTTAGcc                                                                                                                            <  1:395489/251‑1 (MQ=255)                                                                                                     cAAAATCAATGAATTGGTTGGTAGAACATTTGAAGAGAAGGACTACTCTGCTTTTAACTTCTTACAATGGTACGTTGAAGAACAGCACGAAGAAGAAAAATTATTCAGCGGAATTTTGGATAAATTGAATCTTCTTGGCGAAGATGGAAAAGGTTTATTCCTTGTTGATAAAGATTTAGGCGCTTTAGCAGAAAGCAACTAGTCCGGTTTAAATAAAAGGCTAAGTCACATTGATTTAGCCTttcttcttc                                                                                                                  <  1:559018/251‑1 (MQ=255)                                                                                                        aaTCAATGAATTGGTTGGTAGAACATTTGAAGAGAAGGACTACTCTGCTTTTAACTTCTTACAATGGTACGTTGAAGAACAGCACGAAGAAGAAAAATTATTCAGCGGAATTTTGGATAAATTGAATCTTCTTGGCGAAGATGGAAAAGGTTTATTCCTTGTTGATAAAGATTTAGGCGCTTTAGCAGAAAGCAACTAGTCCGGTTTAAATAAAAGGCTAAGTCACATTGATTTAGCCTTTCTTTTTCTCa                                                                                                               >  1:876061/1‑251 (MQ=255)                                                                                                          tCAATGAATTGGTTGGTAGAACATTTGAAGAGAAGGACTACTCTGCTTTTAACTTCTTACAATGGTACGTTGAAGAACAGCACGAAGAAGAAAAATTATTCAGCGGAATTTTGGATAAATTGAATCTTCTTGGCGAAGATGGAAAAGGTTTATTCCTTGTTGATAAAGATTTAGGCGCTTTAGCAGAAAGCAACTAGTCCGGTTTAAATAAAAGGCTAAGTCACATTGATTTAGCCTTTCTTTTTCTCAtt                                                                                                             >  1:272938/1‑251 (MQ=255)                                                                                                          tCAATGAATTGGTTGGTAGAACATTTGAAGAGAAGGACTACTCTGCTTTTAACTTCTTACAATGGTACGTTGAAGAACAGCACGAAGAAGAAAAATTATTCAGCGGAATTTTGGATAAATTGAATCTTCTTGGCGAAGATGGAAAAGGTTTATTCCTTGTTGATAAAGATTTAGGCGCTTTAGCAGAAAGCAACTAGTCCGGTTTAAATAAAAGGCTAAGTCACATTGATTTAGCCTTTCTTTTTCTCAtt                                                                                                             >  1:765217/1‑251 (MQ=255)                                                                                                                         gtAGAACATTTGAAGAGAAGGACTACTCTGCTTTTAACTTCTTACAATGGTACGTTGAAGAACAGCACGAAGAAGAAAAATTATTCAGCGGAATTTTGGATAAATTGAATCTTCTTGGCGAAGATGGAAAAGGTTTATTCCTTGTTGATAAAGATTTAGGCGCTTTAGCAGAAAGCAACTAGTCCGGtt                                                                                                                                                            <  1:494397/189‑1 (MQ=255)                                                                                                                         gtAGAACATTTGAAGAGAAGGACTACTCTGCTTTTAACTTCTTACAATGGTACGTTGAAGAACAGCACGAAGAAGAAAAATTATTCAGCGGAATTTTGGATAAATTGAATCTTCTTGGCGAAGATGGAAAAGGTTTATTCCTTGTTGATAAAGATTTAGGCGCTTTAGCAGAAAGCAACTAGTCCGGTTTAAATAAAAGGCTAAGTCACATTGATTTAGCCTTTCTTTTTCTCATTCCAAAACTTAAtttt                                                                                              >  1:410668/1‑251 (MQ=255)                                                                                                                               cATTTGAAGAGAAGGACTACTCTGCTTTTAACTTCTTACAATGGTACGTTGAAGAACAGCACGAAGAAGAAAAATTATTCAGCGGAATTTTGGATAAATTGAATCTTCTTGGCGAAGATGGAAAAGGTTTATTCCTTGTTGATAAAGATTTAGGCGCTTTAGCAGAAAGCAACTAGTCCGGTTTAAATAAAAGGCTAAGTCACATTGATTTAGCCTTTCTTTTTCTCATTCCAAAACTTAATTTTTAtt                                                                                          >  1:12879/1‑249 (MQ=255)                                                                                                                                 tttGAAGAGAAGGACTACTCTGCTTTTAACTTCTTACAATGGTACGTTGAAGAACAGCACGAAGAAGAAAAATTATTCAGCGGAATTTTGGATAAATTGAATCTTCTTGGCGAAGATGGAAAAGGTTTATTCCTTGTTGATAAAg                                                                                                                                                                                                >  1:634792/1‑145 (MQ=255)                                                                                                                                 tttGAAGAGAAGGACTACTCTGCTTTTAACTTCTTACAATGGTACGTTGAAGAACAGCACGAAGAAGAAAAATTATTCAGCGGAATTTTGGATAAATTGAATCTTCTTGGCGAAGATGGAAAAGGTTTATTCCTTGTTGATAAAGATTTAGGCGCTTTAGCAGAAAGCAACTAGTCCGGTTTAAATAAAAGGCTAAGTCACATTGATTTAGCCTTTCTTTTTCTCATTCCAAAACTTAATTTTTATTCTCa                                                                                      >  1:596680/1‑251 (MQ=255)                                                                                                                                    gAAGAGAAGGACTACTCTGCTTTTAACTTCTTACAATGGTACGTTGAAGAACAGCACGAAGAAGAAAAATTATTCAGCGGAATTTTGGATAAATTGAATCTTCTTGGCGAAGATGGAAAAGGTTTATTCCTTGTTGATAAAGATTTAGGCGCTTTAGCAGAAAGCAACt                                                                                                                                                                     >  1:564521/1‑169 (MQ=255)                                                                                                                                       gagaAGGACTACTCTGCTTTTAACTTCTTACAATGGTACGTTGAAGAACAGCACGAAGAAGAAAAATTATTCAGCGGAATTTTGGATAAATTGAATCTTCTTGGCGAAGATGGAAAAgg                                                                                                                                                                                                                    <  1:156313/119‑1 (MQ=255)                                                                                                                                       gagaAGGACTACTCTGCTTTTAACTTCTTACAATGGTACGTTGAAGAACAGCACGAAGAAGAAAAATTATTCAGCGGAATTTTGGATAAATTGAATCTTCTTGGCGAAGATGGAAAAGGTTTATTCCTTGTTGATAAAGATTTAGGCGCTTTAGCAGAAAGCAACTAGTCCGGTTTAAATAAAAGGCTAAGTCACATTGATTTAGCCTTTCTTTTTCTCATTCCAAAACTTAATTTTTATTCTCAGAAAGt                                                                                >  1:801525/1‑251 (MQ=255)                                                                                                                                         gaAGGACTACTCTGCTTTTAACTTCTTACAATGGTACGTTGAAGAACAGCACGAAGAAGAAAAATTATTCAGCGGAATTTTGGATAAATTGAATCTTCTTGGCGAAGATGGAAAAGGTTTATTCCTTGTTGATAAAGATTTAGGCGCTTTAGCAGAAAGCAACTAGTCCGGTTTAAATAAAAGGCTAAGTCACATTGATTTAGcc                                                                                                                            >  1:689739/1‑205 (MQ=255)                                                                                                                                         gaAGGACTACTCTGCTTTTAACTTCTTACAATGGTACGTTGAAGAACAGCACGAAGAAGAAAAATTATTCAGCGGAATTTTGGATAAATTGAATCTTCTTGGCGAAGATGGAAAAGGTTTATTCCTTGTTGATAAAGATTTAGGCGCTTTAGCAGAAAGCAACTAGTCCGGTTTAAATAAAAGGCTAAGTCACATTGATTTAGCCTTTCTTTTTCTCATTCCAAAACTTAAttt                                                                                               <  1:471609/234‑1 (MQ=255)                                                                                                                                            ggACTACTCTGCTTTTAACTTCTTACAATGGTACGTTGAAGAACAGCACGAAGAAGAAAAATTATTCAGCGGAATTTTGGATAAATTGAATCTTCTTGGCGAAGATGGAAAAGGTTTATTCCTTGTTGATAAAg                                                                                                                                                                                                >  1:310430/1‑134 (MQ=255)                                                                                                                                            ggACTACTCTGCTTTTAACTTCTTACAATGGTACGTTGAAGAACAGCACGAAGAAGAAAAATTATTCAGCGGAATTTTGGATAAATTGAATCTTCTTGGCGAAGATGGAAAAGGTTTATTCCTTGTTGATAAAGATTTAGGCGCTTTAGCAGAAAGCAACTAGTCCGGTTTAAATAAAAGGCTAAGTCACATTGATTTAGCCTTTCTTTTTCTCATTCCAAAACTTAATTTTTATTCTCAGAAAGTGCgg                                                                            <  1:715874/250‑1 (MQ=255)                                                                                                                                            ggACTACTCTGCTTTTAACTTCTTACAATGGTACGTTGAAGAACAGCACGAAGAAGAAAAATTATTCAGCGGAATTTTGGATAAATTGAATCTTCTTGGCGAAGATGGAAAAGGTTTATTCCTTGTTGATAAAGATTTAGGCGCTTTAGCAGAAAGCAACTAGTCCGGTTTAAATAAAAGGCTAAGTCACATTGATTTAGCCTTTCTTTTTCTCATTCCAAAACTTAATTTTTATTCTCAGAAAGTGCGgt                                                                           >  1:789780/1‑251 (MQ=255)                                                                                                                                            ggACTACTCTGCTTTTAACTTCTTACAATGGTACGTTGAAGAACAGCACGAAGAAGAAAAATTATTCAGCGGAATTTTGGATAAATTGAATCTTCTTGGCGAAGATGGAAAAGGTTTATTCCTTGTTGATAAAGATTTAGGCGCTTTAGCAGAAAGCAACTAGTCCGGTTTAAATAAAAGGCTAAGTCACATTGATTTAGCCTTTCTTTTTCTCATTCCAAAACTTAATTTTTATTCTCAGAAAGTGCGgt                                                                           >  1:676480/1‑251 (MQ=255)                                                                                                                                             gACTACTCTGCTTTTAACTTCTTACAATGGTACGTTGAAGAACAGCACGAAGAAGAAAAATTATTCAGCGGAATTTTGGATAAATTGAATCTTCTTGGCGAAGATGGAAAAGGTTTATTCCTTGTTGATAAAGATTTAGGCGCTTTAGCAGAAAGCAACTAGTCCGGTTTAAATAAAAGGCTAAGTCACATTGATTTAGCCTTTCTTTTTCTCATTCCAAAACTTAATTTTTATTCTCAGAAAGTGCgg                                                                            >  1:257531/1‑249 (MQ=255)                                                                                                                                               ctactCTGCTTTTAACTTCTTACAATGGTACGTTGAAGAACAGCACGAAGAAGAAAAATTATTCAGCGGAATTTTGGATAAATTGAATCTTCTTGGCGAAGATGGAAAAGGTTTATTCCTTGTTGATAAAGATTTAGGCGCTTTAGCAGAAAGCAACTAGTCCGGTTtaaat                                                                                                                                                       <  1:85106/172‑1 (MQ=255)                                                                                                                                                 actCTGCTTTTAACTTCTTACAATGGTACGTTGAAGAACAGCACGAAGAAGAAAAATTATTCAGCGGAATTTTGGATAAATTGAATCTTCTTGGCGAAGATGGAAAAGGTTTATTCCTTGTTGATAAAGATTTAGGCGCTTTAGCAGAAAGCAACTAGTCCGGTTTAAATAAAAGGCTAAGTCACATTGAttt                                                                                                                                <  1:178646/193‑1 (MQ=255)                                                                                                                                                      gCTTTTAACTTCTTACAATGGTACGTTGAAGAACAGCACGAAGAAGAAAAATTATTCAGCGGAATTTTGGATAAATTGAAtc                                                                                                                                                                                                                                          >  1:547659/1‑82 (MQ=255)                                                                                                                                                      gCTTTTAACTTCTTACAATGGTACGTTGAAGAACAGCACGAAGAAGAAAAATTATTCAGCGGAATTTTGGATAAATTGAATCTTCTTGGCGAAGATGGAAAAGGTTTATTCCTTGTTGATAAAGATTTAGgc                                                                                                                                                                                        <  1:125776/132‑1 (MQ=255)                                                                                                                                                      gCTTTTAACTTCTTACAATGGTACGTTGAAGAACAGCACGAAGAAGAAAAATTATTCAGCGGAATTTTGGATAAATTGAATCTTCTTGGCGAAGATGGAAAAGGTTTATTCCTTGTTGATAAAGATTTAGGCGCTTTAGCAGAAAGCAACTAGTCCGGTTTAAATAAAAGGCTAAGTCACATTGATTTAGCCTTTCTTTTTCTCATTCCAAAACTTAATTTTTATTCTCAGAAAGTGCGGTGTTTTTTTCa                                                                 >  1:50588/1‑251 (MQ=255)                                                                                                                                                             aCTTCTTACAATGGTACGTTGAAGAACAGCACGAAGAAGAAAAATTATTCAGCGGAATTTTGGATAAATTGAATCTTCTTGGCGAAGATGGAAAAGGTTTATTCCTTGTTGATAAAGATTTAGGCGCTTTAGCAGAAAGCAACTAGTCCGGTTTAAATAAAAGGCTAAGTCACATTGATTTAGCCTTTCTTTTTCTCATTCCAAAACTTAATTTTTATTCTCAGAAAGTGCGGTGTTTTTTTCAAGTGttt                                                          >  1:168805/1‑251 (MQ=255)                                                                                                                                                                  ttACAATGGTACGTTGAAGAACAGCACGAAGAAGAAAAATTATTCAGCGGAATTTTGGATAAATTGAATCTTCTTGGCGAAGATGGAAAAGGTTTATTCCTTGTTGATAAAGATTTAGGCGCTTTAGCAGAAAGCAACTAGTCCGGtt                                                                                                                                                            >  1:259353/1‑148 (MQ=255)                                                                                                                                                                     cAATGGTACGTTGAAGAACAGCACGAAGAAGAAAAATTATTCAGCGGAATTTTGGATAAATTGAATCTTCTTGGCGAAGATGGAAAAGGTTTATTCCTTGTTGATAAAGATTTAGGCGCTTTAGCAGAAAGCAACTAGTCCGGTTTAAATAAAAGGCTAAGTCACATTGATTTAGCCTTTCTTTTTCTCATTCCAAAACTTAATTTTTATTCTCAGAAAGTGCGGTGTTTTTTTCAAGTGTTTTTGCCggt                                                  >  1:726615/1‑251 (MQ=255)                                                                                                                                                                       aTGGTACGTTGAAGAACAGCACGAAGAAGAAAAATTATTCAGCGGAATTTTGGATAAATTGAATCTTCTTGGCGAAGATGGAAAAGGTTTATTCCTTGTTGATAAAGATTTAGGCGCTTTAGCAGAAAGCAACTAGTCCGGttt                                                                                                                                                           <  1:63176/144‑1 (MQ=255)                                                                                                                                                                       aTGGTACGTTGAAGAACAGCACGAAGAAGAAAAATTATTCAGCGGAATTTTGGATAAATTGAATCTTCTTGGCGAAGATGGAAAAGGTTTATTCCTTGTTGATAAAGATTTAGGCGCTTTAGCAGAAAGCAACTAGTCCGGTTTAAATAAAAGGc                                                                                                                                                <  1:795184/155‑1 (MQ=255)                                                                                                                                                                       aTGGTACGTTGAAGAACAGCACGAAGAAGAAAAATTATTCAGCGGAATTTTGGATAAATTGAATCTTCTTGGCGAAGATGGAAAAGGTTTATTCCTTGTTGATAAAGATTTAGGCGCTTTAGCAGAAAGCAACTAGTCCGGTTTAAATAAAAGGCTAAGTCACATTGATTTAGCCTTTCTTTTTCTCATTCCAAAACTTAATTTTTATTCTCAGAAAGTGCGGTGTTTTTTTCAAGTGTTTTTGCCggtgg                                                >  1:56596/1‑251 (MQ=255)                                                                                                                                                                             cGTTGAAGAACAGCACGAAGAAGAAAAATTATTCAGCGGAATTTTGGATAAATTGAATCTTCTTGGCGAAGATGGAAAAGGTTTATTCCTTGTTGATAAAGATTTAGGCGCTTTAGCAGAAAGCAACTAGTCCGGTTTAAATAAAAGGCTAAGTCACATTGATTTAGCCTTTCTTTTTCTCATTCCAAAACTTAATTTTTATTCTCAGAAAGTGCGGTGTTTTTTTCAAGTGTTTTTGCCGGTGGTTGAcc                                          <  1:462852/251‑1 (MQ=255)                                                                                                                                                                               ttGAAGAACAGCACGAAGAAGAAAAATTATTCAGCGGAATTTTGGATAAATTGAATCTTCTTGGCGAAGATGGAAAAGGTTTATTCCTTGTTGATAAAGATTTAGGCGCTTTAGCAGAAAGCAAc                                                                                                                                                                      >  1:146687/1‑125 (MQ=255)                                                                                                                                                                               ttGAAGAACAGCACGAAGAAGAAAAATTATTCAGCGGAATTTTGGATAAATTGAATCTTCTTGGCGAAGATGGAAAAGGTTTATTCCTTGTTGATAAAGATTTAGGCGCTTTAGCAGAAAGCAACTAGTCCGGTTtaaata                                                                                                                                                      <  1:42056/141‑1 (MQ=255)                                                                                                                                                                               ttGAAGAACAGCACGAAGAAGAAAAATTATTCAGCGGAATTTTGGATAAATTGAATCTTCTTGGCGAAGATGGAAAAGGTTTATTCCTTGTTGATAAAGATTTAGGCGCTTTAGCAGAAAGCAACTAGTCCGGTTTAAATAAAAGGCTAAGTc                                                                                                                                          >  1:381400/1‑153 (MQ=255)                                                                                                                                                                               ttGAAGAACAGCACGAAGAAGAAAAATTATTCAGCGGAATTTTGGATAAATTGAATCTTCTTGGCGAAGATGGAAAAGGTTTATTCCTTGTTGATAAAGATTTAGGCGCTTTAGCAGAAAGCAACTAGTCCGGTTTAAATAAAAGGCTAAGTCACATTGATTTAGCCTTTCTTTTTCTCATTCCAAAACTTAATTTTTATTCTCAGAAAGTg                                                                               >  1:141624/1‑212 (MQ=255)                                                                                                                                                                               ttGAAGAACAGCACGAAGAAGAAAAATTATTCAGCGGAATTTTGGATAAATTGAATCTTCTTGGCGAAGATGGAAAAGGTTTATTCCTTGTTGATAAAGATTTAGGCGCTTTAGCAGAAAGCAACTAGTCCGGTTTAAATAAAAGGCTAAGTCACATTGATTTAGCCTTTCTTTTTCTCATTCCAAAACTTAATTTTTATTCTCAGAAAGTGCGGTGTTTTTTTCAAGTGTTTTTGCCGGTGGTTGAc                                           >  1:613390/1‑248 (MQ=255)                                                                                                                                                                               ttGAAGAACAGCACGAAGAAGAAAAATTATTCAGCGGAATTTTGGATAAATTGAATCTTCTTGGCGAAGATGGAAAAGGTTTATTCCTTGTTGATAAAGATTTAGGCGCTTTAGCAGAAAGCAACTAGTCCGGTTTAAATAAAAGGCTAAGTCACATTGATTTAGCCTTTCTTTTTCTCATTCCAAAACTTAATTTTTATTCTCAGAAAGTGCGGTGTTTTTTTCAAGTGTTTTTGCCGGTGGTTGAc                                           >  1:373817/1‑248 (MQ=255)                                                                                                                                                                               ttGAAGAACAGCACGAAGAAGAAAAATTATTCAGCGGAATTTTGGATAAATTGAATCTTCTTGGCGAAGATGGAAAAGGTTTATTCCTTGTTGATAAAGATTTAGGCGCTTTAGCAGAAAGCAACTAGTCCGGTTTAAATAAAAGGCTAAGTCACATTGATTTAGCCTTTCTTTTTCTCATTCCAAAACTTAATTTTTATTCTCAGAAAGTGCGGTGTTTTTTTCAAGTGTTTTTGCCGGTGGTTGAc                                           >  1:15738/1‑248 (MQ=255)                                                                                                                                                                                acaccgcactttctgagaataaAAATTATTCAGCGGAATTTTGGATAAATTGAATCTTCTTGGCGAAGATGGAAAAGGTTTATTCCTTGTTGATAAAGATTTAGGCGCTTTAGCAGAAAGCAACTAGTCCGGTTTAAATAAAAGGCTAAGTCACATTGATTTAGCCTTTCTTTTTCTCATTCCAAAACt                                                                                                     >  1:265622/16‑189 (MQ=255)                                                                                                                                                                                    gaaCAGCACGAAGAAGAAAAATTATTCAGCGGAATTTTGGATAAATTGAATCTTCTTGGCGAAGATGGAAAAGGTTTATTCCTTGTTGATAAAGATTTAGGCGCTTTAGCAGAAAGCAACTAGTCCGGTTTAAATAAAAGGCTAAGTCACATTGATTTAGCCTTTCTTTTTCTCATTCCAAAACTTAATTTTTATTCTCAGAAAGTGCGGTGTTTTTTTCAAGTGTTTTTGCCGGTGGTTGACCTGTTCCa                                   >  1:351329/1‑251 (MQ=255)                                                                                                                                                                                     aaCAGCACGAAGAAGAAAAATTGTTCAGCGGAATTTTGGATAAATTGAATCTTCTTGGCGAAGATGGAAAAGGTTTATTCCTTGTTGATAAAGATTTAGGCGCTTTAGCAGAAAGCAACTAGTCCGGTTTAAATAAAAGGCTAAGTCACATTGATTTAGCCTTTCTTTTTCTCATTCCAAAACTTAATTTTTATTCTCAGAAAGTGCGGTGTTTTTTTCAAGTGTTTTTGCCGGTGGTTGACCTGTTcc                                    <  1:740942/249‑1 (MQ=255)                                                                                                                                                                                     aaCAGCACGAAGAAGAAAAATTATTCAGCGGAATTTTGGATAAATTGAATCTTCTTGGCGAAGATGGAAAAGGTTTATTCCTTGTTGATAAAGATTTAGGCGCTTTAGCAGAAAGCAACTAGTCCGGTTTAAATAAAAGGCTAAGTCACATTGATTTAGCCTTTCTTTTTCTCATTCCAAAACTTAATTTTTATTCTCAGAAAGTGCGGTGTTTTTTTCAAGTGTTTTTGCCGGTGGTTGACCTGTTCCAc                                  <  1:3771/251‑1 (MQ=255)                                                                                                                                                                                          cACGAAGAAGAAAAATTATTCAGCGGAATTTTGGATAAATTGAATCTTCTTGGCGAAGATGGAAAAGGTTTATTCCTTGTTGATAAAGATTTAGGCGCTTTAGCAGAAAGCAACTAGTCCGGTTTAAATAAAAGGCTAAGTCACATTGATTTAGCCTTTCTTTTTCTCATTCCAAAACTTAATTTTTATTCTCAGAAAGTGCGGTGTTTTTTTCAAGTGTTTTTGCCGGTGGTTGACCTGTTCCACTGGc                              <  1:720431/250‑1 (MQ=255)                                                                                                                                                                                                     aaaaTTATTCAGCGGAATTTTGGATAAATTGAATCTTCTTGGCGAAGATGGAAAAGGTTTATTCCTTGTTGATAAAGATTTAGGCGCTTTAGCAGAAAGCAACTAGTCCGGTTTAAATAAAAGGCTAAGTCACATTGATTTAGCCTTTCTTTTTCTCATTCCAAAACTTAATTTTTATTCTCAGAAAGTGCGGTGTTTTTTTCAAGTGTTTTTGCCGGTGGTTGACCTGTTCCACTGGCTTCATAATaaaa                  <  1:577762/251‑1 (MQ=255)                                                                                                                                                                                                      aaaTTATTCAGCGGAATTTTGGATAAATTGAATCTTCTTGGCGAAGATGGAAAAGGTTTATTCCttg                                                                                                                                                                                                         >  1:687540/1‑67 (MQ=255)                                                                                                                                                                                                                     aTTTTGGATAAATTGAATCTTCTTGGCGAAGATGGAAAAGGTTTATTCCTTGTTGATAAAGATTTAGGCGCTTTAGCAGAAAGCAACTAGTCCGGTTTAAATAAAAGGCTAAGTCACATTGATTTAGCCTTTCTTTTTCTCATTCCAAAACTTAATTTTTATTCTCAGAAAGTGCGGTGTTTTTTTCAAGTGTTTTTGCCGGTGGTTGACCTGTTCCACTGGCTTCATAATAAAACGTGCGATAAAAAAAt  >  1:465249/1‑251 (MQ=255)                                                                                                                                                                                                                     aTTTTGGATAAATTGAATCTTCTTGGCGAAGATGGAAAAGGTTTATTCCTTGTTGATAAAGATTTAGGCGCTTTAGCAGAAAGCAACTAGTCCGGTTTAAATAAAAGGCTAAGTCACATTGATTTAGCCTTTCTTTTTCTCATTCCAAAACTTAATTTTTATTCTCAGAAAGTGCGGTGTTTTTTTCAAGTGTTTTTGCCGGTGGTTGACCTGTTCCACTGGCTTCATAATAAAACGTGCGATAAAAAAAt  >  1:571268/1‑251 (MQ=255)                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                               CATTGGCTGTCATTACTGCAATTGATGAACCGCCACACCAATTCGCTTCGTTAAAACAAGTACTTGAATTAACATACGAACATGAAAAATTGATTACTGCCAAAATCAATGAATTGGTTGGTAGAACATTTGAAGAGAAGGACTACTCTGCTTTTAACTTCTTACAATGGTACGTTGAAGAACAGCACGAAGAAGAAAAATTATTCAGCGGAATTTTGGATAAACTGAATCTTCTTGGCGAAGATGGAAAAGGTTTATTCCTTGTTGATAAAGATTTAGGCGCTTTAGCAGAAAGCAACTAGTCCGGTTTAAATAAAAGGCTAAGTCACATTGATTTAGCCTTTCTTTTTCTCATTCCAAAACTTAATTTTTATTCTCAGAAAGTGCGGTGTTTTTTTCAAGTGTTTTTGCCGGTGGTTGACCTGTTCCACTGGCTTCATAATAAAACGTGCGATAAAAAAAT  >  1/1295175‑1295637

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑