Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,294,004	T→A	intergenic (+154874/+99307)	unknown → / ← unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,294,004	0	T	A	100.0%	66.7 / NA	24	intergenic (+154874/+99307)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (11/13);  total (11/13)

CAGCTATCCAATTTTATTAAGACGGATAAAGGCACGTAATAACTGCTGATTAGATAATGCGATACGTGCTTGAATTTATGCTTACACTTCTAAATAAGTCTTGATAAATCAATATTTATTGATGTTACAAGGCTTTATTTCTTCAGTTTTCATCACGTTTAACTAGTAATAGTGACCTGTGAATAACTCTGTGTTTTCATTAAATCGTAGTGTTCATTTACTTTGTCACTAATTTCGCATAACATATATTATGTTAAATAAAATAAATGGTAAGTCACTTCTGGGAATTTGAGGTTTGCTCTCTTTAAAAATGACTGGGTAAAAAAATTTATTTCTTGAAGATTTGATTTTTTGAGCGTTTATGATGATGAGATATTCGTGAGTTTTTAGAGGTAGGTGAAAATACTTCTCTAGCTGAATCTAGGTTTATAAAAATCGACTGATATTCATAGAGGGTTTTTATCGTTATGTACATC  >  1/1293772‑1294247                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                    aaatttcccgcccctgcaaatacccctaaagccacgtaataactgctgactagataatgcgatacgtgcttgaatttatgcttacatTTTTAAATAAGTCTTGATAAATCAATATTTATTGATGTTACAAGGCTTTATTTCTTCAGTTTCCATCACGTTTAACCAGTAAGAGTGACCTGTGAATAACTCTGTGTTTTTATTAATCCGTAGCGTTTATTAACGAAGTCACTAAATTCGCATAACATAtatta                                                                                                                                                                                                                                   <  1:358946‑M2/164‑1 (MQ=255)    tttcccgcccctgcaaatacccctaaagccacgtaataactgctgattagataatgcgatacgtgcttgaatttatgcttacatTTTTAAATAAGTCTTGATAAATCAATATTTATTGATGTTACAAGGCTTTATTTCTTCAGTTTCCATCACGTTTAACCAGTAAGAGTGACCTGTGAATAACTCTGTGTTTTTATTAATCCGTAGCGTTTATTAACGAAGTCACTAAATTCGCATAACATATATTATGt                                                                                                                                                                                                                                <  1:641107‑M2/167‑1 (MQ=255)                    atacccctaaagccacgtaataactgctgattagataatgcgatacgtgcttgaatttatgcttacatTTTTAAATAAGTCTTGATAAATCAATATTTATTGATGTTACAAGGCTTTATTTCTTCAGTTTCCATCACGTTTAACCAGTAAGAGTGACCTGTGAATAACTCTGTGTTTTTATTAATCCGTAGCGTTTATTAACGAAGTCACTAAATTCGCATAACATATATTATGTTAAATAAAATAAATgg                                                                                                                                                                                                                <  1:334688‑M2/183‑1 (MQ=255)                       cccctaaagccacgtaataactgctgattagataatgcgatacgtgcttgaatttatgcttacatTTTTAAATAAGTCTTGATAAATCAATATTTATTGATGTTACAAGGCTTTATTTCTTCAGTTTCCATCACGTTTAACCAGTAAGAGTGACCTGTGAATAACTCTGTGTTTTTATTAATCCGTAGCGTTTATTAACGAAGTCACTAAATTCGCATAACATAtatt                                                                                                                                                                                                                                    <  1:332040‑M2/163‑1 (MQ=255)                       cccctaaagccacgtaataactgctgattagataatgcgatacgtgcttgaatttatgcttacatTTTTAAATAAGTCTTGATAAATCAATATTTATTGATGTTACAAGGCTTTATTTCTTCAGTTTCCATCACGTTTAACCAGTAAGAGTGACCTGTGAATAACTCTGTGTTTTTATTAATCCGTAGCGTTTATTAACGAAGTCACTAAATTCGCATAACATATATTATGTTAAATAAAATAAATGGTaa                                                                                                                                                                                                             >  1:814500‑M2/66‑251 (MQ=255)                                             gctgattagataatgcgatacgtgcttgaatttatgcttacatTTTTAAATAAGTCTTGATAAATCAATATTTATTGATGTTACAAGGCTTTATTTCTTCAGTTTCCATCACGTTTAACCAGTAAGAGTGACCTGTGAATAACTCTGTGTTTTTATTAATCCGTAGCGTTTATTAACGAAGTCACTAAATTCGCATAACATAtatt                                                                                                                                                                                                                                    >  1:591573‑M2/44‑206 (MQ=255)                                                gattagataatgcgatacgtgcttgaatttatgcttacatTTTTAAATAAGTCTTGATAAATCAATATTTATTGATGTTACAAGGCTTTATTTCTTCAGTTTCCATCACGTTTAACCAGTAAGAGTGACCTGTGAATAACTCTGTGTTTTTATTAATCCGTAGCGTTTATTAACGAAGTCACTAAATTCGCATAACatatat                                                                                                                                                                                                                                     <  1:873395‑M2/162‑1 (MQ=255)                                                gattagataatgcgatacgtgcttgaatttatgcttacatTTTTAAATAAGTCTTGATAAATCAATATTTATTGATGTTACAAGGCTTTATTTCTTCAGTTTCCATCACGTTTAACCAGTAAGAGTGACCTGTGAATAACTCTGTGTTTTTATTAATCCGTAGCGTTTATTAACGAAGTCACTAAATTCGCATAACATAtatta                                                                                                                                                                                                                                   <  1:882711‑M2/164‑1 (MQ=255)                                                    agataatgcgatacgtgcttgaatttatgcttacatTTTTAAATAAGTCTTGATAAATCAATATTTATTGATGTTACAAGGCTTTATTTCTTCAGTTTCCATCACGTTTAACCAGTAAGAGTGACCTGTGAATAACTCTGTGTTTTTATTAATCCGTAGCGTTTATTAACGAAGTCACTAAATTCGCATAACatatat                                                                                                                                                                                                                                     >  1:413914‑M2/37‑198 (MQ=255)                                                              atacgtgcttgaatttatgcttacatTTTTAAATAAGTCTTGATAAATCAATATTTATTGATGTTACAAGGCTTTATTTCTTCAGTTTCCATCACGTTTAACCAGTAAGAGTGACCTGTGAATAACTCTGTGTTTTTATTAATCCGTAGCGTTTATTAACGAAGTCACTAAATTCGCATAACATATATTATGTTAAATAAAATAAATGGTAAGTCACTTCTGGGAATTTGAGATTTGCTCTCTTTaaaaa                                                                                                                                                                       <  1:732398‑M2/224‑1 (MQ=255)                                                                 cgtgcttgaatttatgcttacatTTTTAAATAAGTCTTGATAAATCAATATTTATTGATGTTACAAGGCTTTATTTCTTCAGTTTCCATCACGTTTAACCAGTAAGAGTGACCTGTGAATAACTCTGTGTTTTTATTAATCCGTAGCGTTTATTAACGAAGTCACTAAATTCGCATAACATATATTATGTTAAATAAAATAAATGGTAAGTCACTTCTGGGAATTTGAGATTTGCTCTCTTTAAAAATGa                                                                                                                                                                    >  1:684168‑M2/24‑250 (MQ=255)                                                                                                                atatTTATTGATGTTACAAGGCTTTATTTCTTCAGTTTCCATCACGTTTAACCAGTAAGAGTGACCTGTGAATAACTCTGTGTTTTTATTAATCCGTAGCGTTTATTAACGAAGTCACTAAATTCGCATAACATATATTATGt                                                                                                                                                                                                                                >  1:796235/1‑143 (MQ=255)                                                                                                                             ttACAAGGCTTTATTTCTTCAGTTTCCATCACGTTTAACCAGTAAGAGTGACCTGTGAATAACTCTGTGTTTTTATTAATCCGTAGCGTTTATTAACGAAGTCACTAAATTCGCATAACATAtatt                                                                                                                                                                                                                                    <  1:659191/126‑1 (MQ=255)                                                                                                                             ttACAAGGCTTTATTTCTTCAGTTTCCATCACGTTTAACCAGTAAGAGTGACCTGTGAATAACTCTGTGTTTTTATTAATCCGTAGCGTTTATTAACGAAGTCACTAAATTCGCATAACATATATTATGTTAAATAAAATAAATGGTAAGTCACTTCTGGGAATTTGAGATTTGCTCTCTTTaaaaa                                                                                                                                                                       >  1:661888/1‑187 (MQ=255)                                                                                                                                    gCTTTATTTCTTCAGTTTCCATCACGTTTAACCAGTAAGAGTGACCTGTGAATAACTCTGTGTTTTTATTAATCCGTAGCGTTTATTAACGAAGTCACTAAATTCGCATAACat                                                                                                                                                                                                                                         >  1:676356/1‑114 (MQ=255)                                                                                                                                            tcttcAGTTTCCATCACGTTTAACCAGTAAGAGTGACCTGTGAATAACTCTGTGTTTTTATTAATCCGTAGCGTTTATTAACGAAGTCACTAAATTCGCATAACATATATTATGTTAAATAAAATAAATGGTAAGTCACTTCTGGGAATTTGAGATTTGCTCTCTTTAAAAATGACTGGGTAAAAAAATTTATTTCTTGAAGATTTGATTTTTTGAGCGTTTATGATGATGAGATATTCGTGAGTTTTTag                                                                                        <  1:352855/251‑1 (MQ=255)                                                                                                                                             cttcAGTTTCCATCACGTTTAACCAGTAAGAGTGACCTGTGAATAACTCTGTGTTTTTATTAATCCGTAGCGTTTATTAACGAAGTCACTAAATTCGCATAACATATATTATGTTAAATAAAATAAATGGTAAGTCACTTCTGGGAATTTGAGATTTGCTCTCTTTAAAAATGACTGGGTAAAAAAATTTATTTCTTGAAGATTTGATTTTTTGAGCGTTTATGATGATGAGATATTCGTGAGTTTTTaga                                                                                       >  1:313791/1‑251 (MQ=255)                                                                                                                                                               ttAACCAGTAAGAGTGACCTGTGAATAACTCTGTGTTTTTATTAATCCGTAGCGTTTATTAACGAAGTCACTAAATTCGCATAACATATATTATGTTAAATAAAATAAATGGTAAGTCACTTCTGGGAATTTGAGATTTGCTCTCTTTAAAAATGACTGGGTAAAAAAATTTATTTCTTGAAGATTTGATTTTTTGAGCGTTTATGATGATGAGATATTCGTGAGTTTTTAGAGGTAGGTGAAAATACTtc                                                                     <  1:807161/251‑1 (MQ=255)                                                                                                                                                                                                        ttaatccgtagcgtttattaacgaagTCACTAAATTCGCATAACATATATTATGTTAAATAAAATAAATGGTAAGTCACTTCTGGGAATTTGAGATTTGCTCTCTTTAAAAATGACTGGGTAAAAAAATTTATTTCTTGAAGATTTGATTTTTTGAGCGTTTATGATGATGAGATATTCGTGAGTTTTTAGAGGTAGGTGAAAATACTTCTCTAGCTGAATCTAGGTTTATAAAAATCGACTGATATTCAt                            <  1:385787/226‑1 (MQ=255)                                                                                                                                                                                                          aatccgtagcgtttattaacgaagTCACTAAATTCGCATAACATATATTATGTTAAATAAAATAAATGGTAAGTCACTTCTGGGAATTTGAGATTTGCTCTCTTTAAAAATGACTGGGTAAAAAAATTTATTTCTTGAAGATTTGATTTTTTGAGCGTTTATGATGATGCGATATTCGTGAGTTTTTAGAGGTAGGTGAAAATACTTCTCTAGCTGAATCTAGGTTTATAAAAATCGACTGATATTCATag                          >  1:425117/24‑251 (MQ=255)                                                                                                                                                                                                              cgtagcgtttattaacgaagTCACTAAATTCGCATAACATATATTATGTTAAATAAAATAAATGGTAAGTCACTTCTGGGAATTTGAGATTTGCTCTCTTTAAAAATGACTGGGTAAAAAAATTTATTTCTTGAAGATTTGATTTTTTGAGCGTTTATGATGATGAGATATTCGTGAGTTTTTAGAGGTAGGTGAAAATACTTCTCTAGCTGAATCTAGGTTTATAAAAATCGACTGATATTCATAGAggg                      <  1:898298/232‑1 (MQ=255)                                                                                                                                                                                                                                 gTCACTAAATTCGCATAACATATATTATGTTAAATAAAATAAATGGTAAGTCACTTCTGGGAATTTGAGATTTGCTCTCTTTAAAAATGACTGGGTAAAAAAATTTATTTCTTGAAGATTTGATTTTTTGAGCGTTTATGATGATGAGATATTCGTGAGTTTTTAGAGGTAGGTGAAAATACTTCTCTAGCTGAATCTAGGTTTATAAAAATCGACTGATATTCATAGAGGGTTTTTATCGTTATGTACAt   >  1:360393/1‑251 (MQ=255)                                                                                                                                                                                                                                   cACTAAATTCGCATAACATATATTATGTTAAATAAAATAAATGGTAAGTCACTTCTGGGAATTTGAGATTTGCTCTCTTTAAAAATGACTGGGTAAAAAAATTTATTTCTTGAAGATTTGATTTTTTGAGCGTTTATGATGATGAGATATTCGTGAGTTTTTAGAGGTAGGTGAAAATACTTCTCTAGCTGAATCTAGGtt                                                   <  1:397864/201‑1 (MQ=255)                                                                                                                                                                                                                                   cACTAAATTCGCATAACATATATTATGTTAAATAAAATAAATGGTAAGTCACTTCTGGGAATTTGAGATTTGCTCTCTTTAAAAATGACTGGGTAAAAAAATTTATTTCTTGAAGATTTGATTTTTTGAGCGTTTATGATGATGAGATATTCGTGAGTTTTTAGAGGTAGGTGAAAATACTTCTCTAGCTGAATCTAGGTTTATAAAAATCGACTGATATTCATAGAGGGTTTTTATCGTTATGTACATc  >  1:296838/1‑250 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                    CAGCTATCCAATTTTATTAAGACGGATAAAGGCACGTAATAACTGCTGATTAGATAATGCGATACGTGCTTGAATTTATGCTTACACTTCTAAATAAGTCTTGATAAATCAATATTTATTGATGTTACAAGGCTTTATTTCTTCAGTTTTCATCACGTTTAACTAGTAATAGTGACCTGTGAATAACTCTGTGTTTTCATTAAATCGTAGTGTTCATTTACTTTGTCACTAATTTCGCATAACATATATTATGTTAAATAAAATAAATGGTAAGTCACTTCTGGGAATTTGAGGTTTGCTCTCTTTAAAAATGACTGGGTAAAAAAATTTATTTCTTGAAGATTTGATTTTTTGAGCGTTTATGATGATGAGATATTCGTGAGTTTTTAGAGGTAGGTGAAAATACTTCTCTAGCTGAATCTAGGTTTATAAAAATCGACTGATATTCATAGAGGGTTTTTATCGTTATGTACATC  >  1/1293772‑1294247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑