Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,292,997	A→C	intergenic (+153867/+100314)	unknown → / ← unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,292,997	0	A	C	100.0%	165.1 / NA	56	intergenic (+153867/+100314)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (24/32);  total (24/32)

TAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATAGTTTTGGGTGGACTGGCCGAAAACAAGATAACTGAAGGCGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGTAAATCCAAAAATGATACTAGGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTA‑TTTTCCGCTTGC‑GG‑AAAATGCAGGGCGAAGGCTTGCAGCCCGGTAAACAATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCGCGCTTAAATTTAAGCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCTTAAAGCCACGTAATAACTGCTGATTAGATAATGCGAAACGCGCCTTTAATTTATGCTTACATTTCATCAATCACGTCTAACTCTGTGTTTTCATTAAATCGTAGTGTTCATTTACTTTGTCACTAATTTCGCATAATG  >  1/1292755‑1293223                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                   taAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTc                                                                                                                                                                                                                              <  1:21991/251‑1 (MQ=255)     aCGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATg                                                                                                                                                                                                                          <  1:871823/251‑1 (MQ=255)      cgcgACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGt                                                                                                                                                                                                                         <  1:796521/251‑1 (MQ=255)        cgACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTa                                                                                                                                                                                                                       <  1:624089/251‑1 (MQ=255)               gTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCggg                                                                                                                                                                                                                 >  1:315815/1‑250 (MQ=255)                tCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGt                                                                                                                                                                                                                         <  1:92661/241‑1 (MQ=255)                tCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGc                                                                                                                                                                                                                <  1:251811/250‑1 (MQ=255)                tCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCa                                                                                                                                                                                                               >  1:321878/1‑251 (MQ=255)                     gATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCatttt                                                                                                                                                                                                           >  1:791560/1‑250 (MQ=255)                      aTGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCattttta                                                                                                                                                                                                         >  1:18069/1‑251 (MQ=255)                         tAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAGCCATGCTCGATGTTATTCGGGCatttttattt                                                                                                                                                                                                      <  1:721810/251‑1 (MQ=255)                                           aTGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTgcgcgtacctaaatt                                                                                                                                                                                    >  1:212832/1‑241 (MQ=255)                                              tgattgttcttggtggtCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTgcgcgtacc                                                                                                                                                                                          <  1:57031/231‑5 (MQ=255)                                              tgattgttcttggtggtCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCGTACCTAAATTTaa                                                                                                                                                                                 >  1:137407/12‑251 (MQ=255)                                                   gttcttggtggtCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTgcgcgtacctaaat                                                                                                                                                                                     <  1:275550/236‑10 (MQ=255)                                                   gttcttggtggtCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCGTACCTAAATTTAATCgag                                                                                                                                                                            >  1:717953/7‑251 (MQ=255)                                                   gttcttggtggtCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCGTACCTAAATTTAATCgag                                                                                                                                                                            >  1:322230/7‑251 (MQ=255)                                                     tcttggtggtCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCGTACCTAAATTTAATCGAGaa                                                                                                                                                                          >  1:203027/5‑251 (MQ=255)                                                             gtcTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCGTACCTAAATTTAATCGAGAAATGAAAAt                                                                                                                                                                  >  1:850041/3‑251 (MQ=255)                                                                       aaataaaaTAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCGTACCTAAATTTAATCGAGAAATGAAAATTTCCCGccc                                                                                                                                                         >  1:712035/8‑250 (MQ=255)                                                                            aaaTAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCGTACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCaa                                                                                                                                                   <  1:689263/249‑1 (MQ=255)                                                                                         tgAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTAA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCGTACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGc                                                                                                                                      <  1:484113/250‑1 (MQ=255)                                                                                         tgAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTGGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCGTACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGc                                                                                                                                      <  1:575145/250‑1 (MQ=255)                                                                                                ggTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCGTACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGtaa                                                                                                                               >  1:875811/1‑251 (MQ=255)                                                                                                    tttCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCGTACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGtaataa                                                                                                                            >  1:521567/1‑250 (MQ=255)                                                                                                    tttCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCGTACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAAc                                                                                                                           <  1:41444/251‑1 (MQ=255)                                                                                                       cATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCGTACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAActgc                                                                                                                        <  1:498703/251‑1 (MQ=255)                                                                                                                cTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCGTACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGAt                                                                                                               <  1:790901/251‑1 (MQ=255)                                                                                                                        gtttgatagttttttaacgaaattGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCGTACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGCGat                                                                                                       >  1:297496/24‑250 (MQ=255)                                                                                                                                   aaaatCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCGTACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGCGAtacgtgcttgaa                                                                                            <  1:341661‑M1/250‑13 (MQ=255)                                                                                                                                               tGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCGTACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAAc                                                                                                                           >  1:400230/1‑208 (MQ=255)                                                                                                                                                  tACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCGTACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGc                                                                                                          <  1:531319/222‑1 (MQ=255)                                                                                                                                                  tACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCGTACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGCGAtacg                                                                                                    <  1:613501‑M1/228‑5 (MQ=255)                                                                                                                                                  tACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCGTACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGCGAtac                                                                                                     >  1:303813‑M1/1‑224 (MQ=255)                                                                                                                                                   aCTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCGTACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGCGAtacgtgcttgaatttatgcttacatttt                                                                            >  1:739068‑M1/1‑223 (MQ=255)                                                                                                                                                    ctaagacagATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCGTACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGCGAtacgtgcttgaatttatgcttacattttt                                                                           >  1:756241‑M1/5‑222 (MQ=255)                                                                                                                                                        gACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCGTACCTAAATTTaat                                                                                                                                                                                >  1:89368/1‑145 (MQ=255)                                                                                                                                                        gACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCGTACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTaa                                                                                                                                         <  1:723725/185‑1 (MQ=255)                                                                                                                                                        gACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCGTACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGCGAtacgtgcttgaattta                                                                                        >  1:791023‑M1/1‑218 (MQ=255)                                                                                                                                                          cAGATATAGTGATTTTCCTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCAGGCAGAATGTTATTCGGGCATTTTTATTTTTTCCGCGTCCATAAATTAAATCGAGAATTGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGCGatac                                                                                                     <  1:226758/219‑4 (MQ=255)                                                                                                                                                             atatAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCGTACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGAt                                                                                                               <  1:171824/206‑1 (MQ=255)                                                                                                                                                                   tGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGGTTGCAGCCCGATAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCGTCCCAAAATTTAATCAAGAAATAAAAATTTACCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGCGATACGTG‑CTTGAATTTATGCTTACAtttttaaataagtcttgata                                                           <  1:711642/251‑18 (MQ=16)                                                                                                                                                                    gATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCatttttattttt                                                                                                                                                                                                    <  1:524522/114‑1 (MQ=255)                                                                                                                                                                     aTTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCGTACCTAAATTTaat                                                                                                                                                                                <  1:162350/133‑2 (MQ=255)                                                                                                                                                                     aTTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCGTACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAAc                                                                                                                           >  1:799159/1‑186 (MQ=255)                                                                                                                                                                     aTTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCGTACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGCGAtacgtgcttgaatttatgcttacatttttaaataagtcttgataaa                                                          <  1:268366‑M1/251‑47 (MQ=255)                                                                                                                                                                     aTTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCGTACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGCGAtacgtgcttgaatttatgcttacatttttaaataagtc                                                                  <  1:426022‑M1/243‑39 (MQ=255)                                                                                                                                                                      ttttACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCA‑AAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCGTACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGCGAtacgtgcttgaatttatgcttacatttttaaataagtcttgataaat                                                         >  1:784907‑M1/1‑204 (MQ=255)                                                                                                                                                                               aaGTGAAACTTGTCTAACTATTTTTGAGCAAGC‑TCAAAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCGTACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGCGAtacgtgcttgaatttatgcttacatttttaaataagtcttgataaatcaatattta                                                >  1:844796‑M1/1‑195 (MQ=255)                                                                                                                                                                                               aactatttttgggcaagctcaaaaatgtagggcgaaggcttgcagcCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCGTACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGCGAtacgtgcttgaatttatgcttacatttttaaataagtcttgataaatcaatatttattgatgttacaagg                                  >  1:346280‑M1/22‑180 (MQ=255)                                                                                                                                                                                               aactatttttgagcaagctcaaaaatgtagggcgaaggcttgcagcCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCGTACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGCGAtacgtgcttgaatttatgcttacatttttaaataagtcttgataaatcaatatttattgatgttacaaggc                                 <  1:236940‑M1/230‑72 (MQ=255)                                                                                                                                                                                                               gctcaaaaaTGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCGTACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATa                                                                                                                                                <  1:720040/119‑1 (MQ=255)                                                                                                                                                                                                                  caaaaaTGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCGTACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAg                                                                                                                                       <  1:233728/128‑1 (MQ=255)                                                                                                                                                                                                                               cGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCGTACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGCGAtacgtgcttgaatttatgcttacatttttaaataagtcttgataaatcaatatttattgatgttacaaggctttatttcttcagtttccatcacgtttaacc  <  1:367120‑M1/250‑103 (MQ=255)                                                                                                                                                                                                                                           gCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCGTACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGCGAtacgtgcttgaatttatgcttacatttttaaataagtcttgataaatcaatatttattgatgttacaa                                    <  1:828074‑M1/204‑69 (MQ=255)                                                                                                                                                                                                                                            cccGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCGTACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGATAATGCGAtacgtgcttgaatttatgcttacatttttaaataagtcttgataaatcaatat                                                   <  1:530159‑M1/188‑54 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                   TAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATAGTTTTGGGTGGACTGGCCGAAAACAAGATAACTGAAGGCGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGTAAATCCAAAAATGATACTAGGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTA‑TTTTCCGCTTGC‑GG‑AAAATGCAGGGCGAAGGCTTGCAGCCCGGTAAACAATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCGCGCTTAAATTTAAGCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCTTAAAGCCACGTAATAACTGCTGATTAGATAATGCGAAACGCGCCTTTAATTTATGCTTACATTTCATCAATCACGTCTAACTCTGTGTTTTCATTAAATCGTAGTGTTCATTTACTTTGTCACTAATTTCGCATAATG  >  1/1292755‑1293223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑