Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,292,885	T→A	intergenic (+153755/+100426)	unknown → / ← unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,292,885	0	T	A	100.0%	162.7 / NA	55	intergenic (+153755/+100426)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (26/29);  total (26/29)

ACATTTAAAAAAGTAGCCGGTTACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATAGTTTTGGGTGGACTGGCCGAAAACAAGATAACTGAAGGCGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGTAAATCCAAAAATGATACTAGGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTA‑TTTTCCGCTTGC‑GGAAAATGCAGGGCGAAGGCTTGCAGCCCGGTAAACAATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCGCGCTTAAATTTAAGCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCTTAAAGCCACGTAATAACTGCTGATTAGAT  >  1/1292705‑1293114                                                                                                                                                                                     |                                                                                                                                                                                                                                        atctaattttgttaagacggatACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTc                                                                                                                                                                                <  1:735103/217‑1 (MQ=255) atctaattttgttaagacggatACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTA‑tttttga                                                                                                                                                                  >  1:897988/22‑248 (MQ=255)      attttgttaagacggatACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTA‑tttttgagcaag                                                                                                                                                             >  1:47361/17‑243 (MQ=255)                gacggatACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGAc                                                                                                                                                                                                                  <  1:356024/183‑1 (MQ=255)                gacggatACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTa                                                                                                                                                                              <  1:645917/219‑1 (MQ=255)                gacggatACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTA‑tttttgagcaagctcaaaaatg                                                                                                                                                   >  1:631333/7‑233 (MQ=255)                   ggatACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAATAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTA‑tttttgagcaagctcaaaaatgtag                                                                                                                                                >  1:92644/4‑230 (MQ=255)                   ggatACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGAc                                                                                                                                                                                                                  >  1:126598/4‑186 (MQ=255)                    gatACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTg                                                                                                                                                                                                        >  1:710266/3‑195 (MQ=255)                    gatACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTA‑tttttgagcaagctcaaaaatgtagg                                                                                                                                               >  1:60513/3‑229 (MQ=255)                      tACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTg                                                                                                                                                                                                        <  1:573317/193‑1 (MQ=255)                      tACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTAc                                                                                                                                                                                                 >  1:740583/1‑200 (MQ=255)                                     atcTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCaaaa                                                                                                                                                                                                                               <  1:858884/155‑1 (MQ=255)                                            acacTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCAAAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAAc                                                                                                                        <  1:828510/251‑1 (MQ=255)                                            acacTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTCTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCAAAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAAc                                                                                                                        <  1:639394/251‑1 (MQ=255)                                             cacTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCAAAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAAcc                                                                                                                       <  1:119271/251‑2 (MQ=255)                                              acTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCAAAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAAcca                                                                                                                      <  1:283417/251‑3 (MQ=255)                                               cTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGAc                                                                                                                                                                                                                  <  1:700016/158‑1 (MQ=255)                                                   taAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCAAAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTc                                                                                                                 <  1:21991/251‑1 (MQ=255)                                                     aaaCGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCAAAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAAc                                                                                                                        >  1:882242/1‑242 (MQ=255)                                                       aCGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCAAAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATg                                                                                                             <  1:871823/251‑1 (MQ=255)                                                        cgcgACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCAAAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGt                                                                                                            <  1:796521/251‑1 (MQ=255)                                                          cgACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCAAAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTa                                                                                                          <  1:624089/251‑1 (MQ=255)                                                                 gTCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCAAAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCggg                                                                                                    >  1:315815/1‑250 (MQ=255)                                                                  tCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGatat                                                                                                                                                                                                            >  1:119379/1‑145 (MQ=255)                                                                  tCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCAAAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGt                                                                                                            <  1:92661/241‑1 (MQ=255)                                                                  tCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCAAAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGc                                                                                                   <  1:251811/250‑1 (MQ=255)                                                                  tCACAGATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCAAAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCa                                                                                                  >  1:321878/1‑251 (MQ=255)                                                                       gATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCaaaaa                                                                                                                                                                                                                              >  1:435792/1‑122 (MQ=255)                                                                       gATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGa                                                                                                                                                                                                       <  1:472060/145‑1 (MQ=255)                                                                       gATGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCAAAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCatttt                                                                                              >  1:791560/1‑250 (MQ=255)                                                                        aTGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACt                                                                                                                                                                                    <  1:595573/163‑1 (MQ=255)                                                                        aTGTAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCAAAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCattttta                                                                                            >  1:18069/1‑251 (MQ=255)                                                                           tAAGTGTAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCAAAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAGCCATGCTCGATGTTATTCGGGCatttttattt                                                                                         <  1:721810/251‑1 (MQ=255)                                                                              gtgtAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTg                                                                                                                                                                                                        >  1:645560/1‑137 (MQ=255)                                                                                gtAAAAAATGGCGATGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAg                                                                                                                                                                                                          <  1:602292/133‑1 (MQ=255)                                                                                             aTGTGATTGTTCTTGGTGGTCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCAAAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTgcgcgtacctaaatt                                                                       >  1:212832/1‑241 (MQ=255)                                                                                                tgattgttcttggtggtCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCAAAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTgcgcgtacc                                                                             <  1:57031/231‑5 (MQ=255)                                                                                                tgattgttcttggtggtCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCAAAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCGTACCTAAATTTaa                                                                    >  1:137407/12‑251 (MQ=255)                                                                                                     gttcttggtggtCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCAAAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTgcgcgtacctaaat                                                                        <  1:275550/236‑10 (MQ=255)                                                                                                     gttcttggtggtCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCAAAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCGTACCTAAATTTAATCgag                                                               >  1:717953/7‑251 (MQ=255)                                                                                                     gttcttggtggtCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCAAAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCGTACCTAAATTTAATCgag                                                               >  1:322230/7‑251 (MQ=255)                                                                                                       tcttggtggtCTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCAAAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCGTACCTAAATTTAATCGAGaa                                                             >  1:203027/5‑251 (MQ=255)                                                                                                               gtcTGGCTGAAAATAAAATAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCAAAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCGTACCTAAATTTAATCGAGAAATGAAAAt                                                     >  1:850041/3‑251 (MQ=255)                                                                                                                         aaataaaaTAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCAAAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAAc                                                                                                                        >  1:587545/8‑174 (MQ=255)                                                                                                                         aaataaaaTAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCAAAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCGTACCTAAATTTAATCGAGAAATGAAAATTTCCCGccc                                            >  1:712035/8‑250 (MQ=255)                                                                                                                              aaaTAACGGAAGGTGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCAAAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCGTACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCaa                                      <  1:689263/249‑1 (MQ=255)                                                                                                                                          gtgAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAAc                                                                                                                                                                            <  1:181126/103‑1 (MQ=255)                                                                                                                                           tgAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTAAAAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCGTACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGc                         <  1:484113/250‑1 (MQ=255)                                                                                                                                           tgAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTGGTCTAACTATTTTTGAGCAAGCTCAAAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCGTACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGc                         <  1:575145/250‑1 (MQ=255)                                                                                                                                                  ggTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCAAAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCGTACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGtaa                  >  1:875811/1‑251 (MQ=255)                                                                                                                                                      tttCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCAAAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCGTACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGtaataa               >  1:521567/1‑250 (MQ=255)                                                                                                                                                      tttCATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCAAAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCGTACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAAc              <  1:41444/251‑1 (MQ=255)                                                                                                                                                         cATTTCTTCCTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCAAAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCGTACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAActgc           <  1:498703/251‑1 (MQ=255)                                                                                                                                                                  cTAAAGGTTTTTTAACGGGAAAATCCAAAAATGATACTAAGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTATTTTTGAGCAAGCTCAAAAATGTAGGGCGAAGGCTTGCAGCCCGGTAAACCATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCGTACCTAAATTTAATCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCCTAAAGCCACGTAATAACTGCTGATTAGAt  <  1:790901/251‑1 (MQ=255)                                                                                                                                                                                     |                                                                                                                                                                                                                                        ACATTTAAAAAAGTAGCCGGTTACAGGTGTTAATCAATCTCCGACACTTATAAAACGCGACATTGTCACAGATGTAAGTGTAAAAAATGGCGATGTGATAGTTTTGGGTGGACTGGCCGAAAACAAGATAACTGAAGGCGAAACAGGTTTTTCATTTCTTCCTAAAGGTTTTTTAACGGGTAAATCCAAAAATGATACTAGGACAGATATAGTGATTTTACTCCAAGTGAAACTTGTCTAACTA‑TTTTCCGCTTGC‑GGAAAATGCAGGGCGAAGGCTTGCAGCCCGGTAAACAATGCTCGATGTTATTCGGGCATTTTTATTTTTTGCGCGCGCTTAAATTTAAGCGAGAAATGAAAATTTCCCGCCCCTGCAAATACCCTTAAAGCCACGTAATAACTGCTGATTAGAT  >  1/1292705‑1293114

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑