Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,286,084	G→A	intergenic (+146954/+107227)	unknown → / ← unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,286,084	0	G	A	100.0%	191.4 / NA	67	intergenic (+146954/+107227)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/42);  total (25/42)

GTTACATGAATTGGAACAACGTCTGAATCAACAACAAAGTGCGGTCAGATTATTAAACGATTTTAATCAACGCGCCAATTTGTCTTTAGAAACCGCAGATGAATTAGAAGAATTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCGCCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAAGAGCGTGAATTCACCATGGAGCGCGATCAGCTTGAGTTACAACGTCAGCAATTAGATGAGCAAATCAGCCGATTAAGTCAGCCCGACGGATCCGAAGATGCCCGTTTGAACGTACTTGCGG  >  1/1285841‑1286316                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                         gTTACATGAATTGGAACAACGTCTGAATCAACAACAAAGTGCGGTCAGATTATTAAACGATTTTAATCAACGCGCCAATTTGTCTTTAGAAACCGCAGATGAATTAGAAGAATTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCAt                                                                                                                                                                                                                                   >  1:260172/1‑251 (MQ=255)  ttACATGAATTGGAACAACGTCTGAATCAACAACAAAGTGCGGTCAGATTATTAAACGATTTTAATCAACGCGCCAATTTGTCTTTAGAAACCGCAGATGAATTAGAAGAATTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCAt                                                                                                                                                                                                                                   <  1:868472/250‑1 (MQ=255)      aTGAATTGGAACAACGTCTGAATCAACAACAAAGTGCGGTCAGATTATTAAACGATTTTAATCAACGCGCCAATTTGTCTTTAGAAACCGCAGATGAATTAGAAGAATTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTc                                                                                                                                                                                                                              <  1:409071/251‑1 (MQ=255)         aaTTGGAACAACGTCTGAATCAACAACAAAGTGCGGTCAGATTATTAAACGATTTTAATCAACGCGCCAATTTGTCTTTAGAAACCGCAGATGAATTAGAAGAATTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTc                                                                                                                                                                                                                              <  1:803829/248‑1 (MQ=255)         aaTTGGAACAACGTCTGAATCAACAACAAAGTGCGGTCAGATTATTAAACGATTTTAATCAACGCGCCAATTTGTCTTTAGAAACCGCAGATGAATTAGAAGAATTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACg                                                                                                                                                                                                                           >  1:156816/1‑251 (MQ=255)         aaTTGGAACAACGTCTGAATCAACAACAAAGTGCGGTCAGATTATTAAACGATTTTAATCAACGCGCCAATTTGTCTTTAGAAACCGCAGATGAATTAGAAGAATTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACg                                                                                                                                                                                                                           >  1:720930/1‑251 (MQ=255)           ttGGAACAACGTCTGAATCAACAACAAAGTGCGGTCAGATTATTAAACGATTTTAATCAACGCGCCAATTTGTCTTTAGAAACCGCAGATGAATTAGAAGAATTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGc                                                                                                                                                                                                                         <  1:752326/251‑1 (MQ=255)                    cGTCTGAATCAACAACAAAGTGCGGTCAGATTATTAAACGATTTTAATCAACGCGCCAATTTGTCTTTAGAAACCGCAGATGAATTAGAAGAATTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAgcgg                                                                                                                                                                                                                 >  1:810840/1‑250 (MQ=255)                           aTCAACAACAAAGTGCGGTCAGATTATTAAACGATTTTAATCAACGCGCCAATTTGTCTTTAGAAACCGCAGATGAATTAGAAGAATTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGaa                                                                                                                                                                                                         >  1:772989/1‑251 (MQ=255)                           aTCAACAACAAAGTGCGGTCAGATTATTAAACGATTTTAATCAACGCGCCAATTTGTCTTTAGAAACCGCAGATGAATTAGAAGAATTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGaa                                                                                                                                                                                                         <  1:804601/251‑1 (MQ=255)                             caacaacaaAGTGCGGTCAGATTATTAAACGATTTTAATCAACGCGCCAATTTGTCTTTAGAAACCGCAGATGAATTAGAAGAATTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACg                                                                                                                                                                                                       >  1:18531/1‑251 (MQ=255)                                       gTGCGGTCAGATTATTAAACGATTTTAATCAACGCGCCAATTTGTCTTTAGAAACCGCAGATGAATTAGAAGAATTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCATAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGaa                                                                                                                                                                                                         <  1:430278/239‑1 (MQ=255)                                             tCAGATTATTAAACGATTTTAATCAACGCGCCAATTTGTCTTTAGAAACCGCAGATGAATTAGAAGAATTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAg                                                                                                                                                                                        <  1:289809/250‑1 (MQ=255)                                              cAGATTATTAAACGATTTTAATCAACGCGCCAATTTGTCTTTAGAAACCGCAGATGAATTAGAAGAATTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCg                                                                                                                                                                                      >  1:897482/1‑251 (MQ=255)                                                 attattAAACGATTTTAATCAACGCGCCAATTTGTCTTTAGAAACCGCAGATGAATTAGAAGAATTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCgg                                                                                                                                                                                     <  1:581563/249‑1 (MQ=255)                                                  ttattaAACGATTTTAATCAACGCGCCAATTTGTCTTTAGAAACCGCAGATGAATTAGAAGAATTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTg                                                                                                                                                                                                   <  1:845995/234‑1 (MQ=255)                                                            gttttAATCAACGCGCCAATTTGTCTTTAGAAACCGCAGATGAATTAGAAGAATTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAGAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGc                                                                                                                                                                         >  1:428306/2‑250 (MQ=255)                                                            aTTTTAATCAACGCGCCAATTTGTCTTTAGAAACCGCAGATGAATTAGAAGAATTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGaa                                                                                                                                                                                                         >  1:384106/1‑218 (MQ=255)                                                            aTTTTAATCAACGCGCCAATTTGTCTTTAGAAACCGCAGATGAATTAGAAGAATTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGc                                                                                                                                                                                       >  1:593846/1‑236 (MQ=255)                                                                         cgcCAATTTGTCTTTAGAAACCGCAGATGAATTAGAAGAATTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCg                                                                                                                                                           <  1:174084/251‑1 (MQ=255)                                                                              aTTTGTCTTTAGAAACCGCAGATGAATTAGAAGAATTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGtgatg                                                                                                                                                      <  1:639464/251‑1 (MQ=255)                                                                                 tGTCTTTAGAAACCGCAGATGAATTAGAAGAATTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAAt                                                                                                                                                   >  1:421559/1‑251 (MQ=255)                                                                                          aaaCCGCAGATGAATTAGAAGAATTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGc                                                                                                                                            <  1:241748/249‑1 (MQ=255)                                                                                                      aaTTAGAAGAATTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAgcggcg                                                                                                                                                                                                               <  1:195682/170‑1 (MQ=255)                                                                                                      aaTTAGAAGAATTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGtt                                                                                                                              <  1:577406/251‑1 (MQ=255)                                                                                                            aagaaTTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAagag                                                                                                                        <  1:28656/251‑1 (MQ=255)                                                                                                            aagaaTTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAag                                                                                                                          <  1:652047/249‑1 (MQ=255)                                                                                                                aTTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAaga                                                                                                                         >  1:512392/1‑246 (MQ=255)                                                                                                                aTTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAAGAGCGTg                                                                                                                    >  1:758395/1‑251 (MQ=255)                                                                                                                     aTGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAag                                                                                                                          >  1:198749/1‑240 (MQ=255)                                                                                                                     aTGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAAGAGCGTGAATTc                                                                                                               <  1:121115/251‑1 (MQ=255)                                                                                                                                gAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAAGAGCGTGAATTCACCATgg                                                                                                        <  1:550733/247‑1 (MQ=255)                                                                                                                                    cTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGAc                                                                                                                                                                     <  1:92846/182‑1 (MQ=255)                                                                                                                                         aTTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAag                                                                                                                          <  1:614777/220‑1 (MQ=255)                                                                                                                                                aTTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAAGAGCGTGAATTCACCATGGAGCGCGATCAGCTTGAGtt                                                                                     <  1:274138/250‑1 (MQ=255)                                                                                                                                                aTTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTAAAAGAGCGTGAATTCACCATGGAGCGCGATCAGCTTGAGtt                                                                                     <  1:817125/250‑1 (MQ=255)                                                                                                                                                            aaCTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAAGAGCGTGAATTCACCATGGAGCGCGATCAGCTTGAGTTACAACGTCAGCaa                                                                        <  1:204086/251‑1 (MQ=255)                                                                                                                                                            aaCTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAAGAGCGTGAATTCACCATGGAGCGCGATCAGCTTGAGTTACAACGTCAGCaa                                                                        <  1:377766/251‑1 (MQ=255)                                                                                                                                                              cTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAAGAGCGTGAATTCACCATGGAGCGCGATCAGCTTGAGTTACAACGTCAGCAAtt                                                                      >  1:554712/1‑251 (MQ=255)                                                                                                                                                                ttCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATC‑AAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAAGAGCGTGAATTCACCATGGAGCGCGATCAGCTTGAGTTACAACGTCAGCAATTAGa                                                                   <  1:461648/251‑1 (MQ=255)                                                                                                                                                                 tCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGGAGTTTTTGAGGAGAGCGCCCGTAAAGCACCGGCATGGCTCACGGCACATGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGTCAAGGCGTGATGAATTTCATGCAGGCACAGGTGGTTAAAGAGCGTGAATTCACCATGGAGCGCGATCAGCTTGAGGTACAACGTCAGCAATTAGa                                                                   <  1:257693/251‑1 (MQ=255)                                                                                                                                                                 tCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAAGAGCGTGAATTCACCATGGAGCGCGATCAGCTTGAGTTACAACGTCAGCAATTAGa                                                                   <  1:419793/251‑1 (MQ=255)                                                                                                                                                                 tCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAAGAGCGTGAATTCACCATGGAGCGCGATCAGCTTGAGTTACAACGTCAGCAATTAGa                                                                   <  1:200895/251‑1 (MQ=255)                                                                                                                                                                 tCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAAGAGCGTGAATTCACCATGGAGCGCGATCAGCTTGAGTTACAACGTCAGCAATTAGa                                                                   <  1:386418/251‑1 (MQ=255)                                                                                                                                                                 tCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGAATGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAAGAGCGTGAATTCACCATGGAGCGCGATCAGCTTGAGTTACAACGTCAGCAATTAGa                                                                   <  1:106295/251‑1 (MQ=255)                                                                                                                                                                  cGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAAGAGCGTGAATTCACCATGGAGCGCGATCAGCTTGAGTTACAACGTCAGCAATTAGAt                                                                  <  1:585995/251‑1 (MQ=255)                                                                                                                                                                    gACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAAGAGCGTGAATTCACCATGGAGCGCGATCAGCTTGAGTTACAACGTCAGCAATTAGATg                                                                 <  1:754129/250‑1 (MQ=255)                                                                                                                                                                    gACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAAGAGCGTGAATTCACCATGGAGCGCGATCAGCTTGAGTTACAACGTCAGCAATTAGATGa                                                                >  1:138095/1‑251 (MQ=255)                                                                                                                                                                        tGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAAGAGCGTGAATTCACCATGGAGCGCGATCAGCTTGAGt                                                                                      >  1:840435/1‑225 (MQ=255)                                                                                                                                                                          gTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAAGAGCGTGAATTCACCATGGAGCGCGATCAGCTTGAg                                                                                       <  1:861633/222‑1 (MQ=255)                                                                                                                                                                          gTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAAGAGCGTGAATTCACCATGGAGCGCGATCAGCTTGAGTTACAACg                                                                               <  1:85962/230‑1 (MQ=255)                                                                                                                                                                          gTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAAGAGCGTGAATTCACCATGGAGCGCGATCAGCTTGAGTTACAACGTCAGCAATTAGATGAGCAAAt                                                          >  1:771076/1‑251 (MQ=255)                                                                                                                                                                                    gTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAAGAGCGTGAATTCACCATGGAGCGCGATCAGCTTGAGTTACAACGTCAGCAATTAGATGAGCAAATCAGCCGATTa                                                >  1:658467/1‑251 (MQ=255)                                                                                                                                                                                        aaCCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAAGAGCGTGAATTCACCATGGAGCGCGATCAGCTTGAGTTACAACGTCAGCAATTAGATGAGCAAATCAGCCGATTAAGTc                                            <  1:877187/251‑1 (MQ=255)                                                                                                                                                                                        aaCCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAAGAGCGTGAATTCACCATGGAGCGCGATCAGCTTGAGTTACAACGTCAGCAATTAGATGAGCAAATCAGCCGATTAAGTc                                            <  1:796506/251‑1 (MQ=255)                                                                                                                                                                                        aaCCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAAGAGCGTGAATTCACCATGGAGCGCGATCAGCTTGAGTTACAACGTCAGCAATTAGATGAGCAAATCAGCCGATTAAGTc                                            <  1:274876/251‑1 (MQ=255)                                                                                                                                                                                          cccTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGAc                                                                                                                                                                     <  1:279294/128‑1 (MQ=255)                                                                                                                                                                                                 ccAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAag                                                                                                                          <  1:429629/164‑1 (MQ=255)                                                                                                                                                                                                             aaaTTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAAGAGCGTGAATTCACCATGGAgc                                                                                                     >  1:318335/1‑173 (MQ=255)                                                                                                                                                                                                             aaaTTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAAGAGCGTGAATTCACCATGGAGCGCGATCAGCTTGAGTTACAACGTCAGCAATTAGATGAGCAAATCAGCCGATTAAGTCAGCCCGACGGATCCGAAGATg                       >  1:800034/1‑251 (MQ=255)                                                                                                                                                                                                               aTTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAAGAGCGTGAATTCACCATGGAGCGCGATCAGCTTGAGTTACAACGTCAGCAATTAGATGAGCAAATCAGCCGATTAAGTCAGCCCGACGGATCCGAAGATGcc                     <  1:151512/251‑1 (MQ=255)                                                                                                                                                                                                                  tGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGaa                                                                                                                                                                                                         >  1:713622/1‑68 (MQ=255)                                                                                                                                                                                                                        cACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAAGAGCGTGAATTCACCATGGAGCGCGATCAGCTTGAGTTACaa                                                                                 <  1:128939/182‑1 (MQ=255)                                                                                                                                                                                                                        cACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAAGAGCGTGAATTCACCATGGAGCGCGATCAGCTTGAGTTACAACGTCAGCAATTAGATGAGCAAATCAGCCGATTAAGTCAGCCCGACGGATCCGAAGATGCCCGTTTGaa             >  1:646006/1‑250 (MQ=255)                                                                                                                                                                                                                                 aGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAag                                                                                                                          >  1:156948/1‑132 (MQ=255)                                                                                                                                                                                                                                  ggAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAAGAGCGTGAATTCACCATGGAGCGCGATCAGCTTGAGTTACAACGTCAGCAATTAGATGAGCAAATCAGCCGATTAAGTCAGCCCGACGGATCCGAAGATGCCCGTTTGAACGTACTTg     >  1:688823/1‑248 (MQ=255)                                                                                                                                                                                                                                   gAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAAGAGCGTGAATTCACCATGGAGCGCGATCAGCTTGAGTTACAACGTCAGCAATTAGATGAGCAAATCAGCCGATTAAGTCAGCCCGACGGATCCGAAGATGCCCGTTTGAACGTACTTGCgg  <  1:827620/250‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                         GTTACATGAATTGGAACAACGTCTGAATCAACAACAAAGTGCGGTCAGATTATTAAACGATTTTAATCAACGCGCCAATTTGTCTTTAGAAACCGCAGATGAATTAGAAGAATTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCGCCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAAGAGCGTGAATTCACCATGGAGCGCGATCAGCTTGAGTTACAACGTCAGCAATTAGATGAGCAAATCAGCCGATTAAGTCAGCCCGACGGATCCGAAGATGCCCGTTTGAACGTACTTGCGG  >  1/1285841‑1286316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑