Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,285,735	T→C	intergenic (+146605/+107576)	unknown → / ← unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,285,735	0	T	C	100.0%	218.2 / NA	75	intergenic (+146605/+107576)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (39/36);  total (39/36)

GGCGTTGGATGCGCAACAAACCCGCGCATTGCAATATCAACAAGCCATTCAAGCCTTAGAAAAAGCCAAAACGTTGTGCGGTTTGGCGGATTTAGCCGTGAAAAATGTGGATGTTTACCACGAAGAATTTGAAGCGCAAGCGGAAGTGCTAACGGATAAAGTGCTTGAACTTGAACAAAAAATGTCTATTTCCGAAGCGGCAAAAACGCAATTCGACAAAGCCTATCAGTTGGTTTGCAAAATTGTCGGCGATGTACCTCGTTCTGCTGCGTGGGAAAGTGCGAAAGAATTGTTGCGTGAATATCCGACACAAAAACTGCAAGCACAGCAAACACCGCAACTACGCGCCAAGTTACATGAATTGGAACAACGTCTGAATCAACAACAAAGTGCGGTCAGATTATTAAACGATTTTAATCAACGCGCCAATTTGTCTTTAGAAACCGCAGATGAATTAGAAGAATTTTATGGTGAACAGGAAGCTTTAAT  >  1/1285490‑1285978                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    ggCGTTGGATGCGCAACAAACCCGCGCATTGCAATATCAACAAGCCATTCAAGCCTTAGAAAAAGCCAAAACGTTGTGCGGTTTGGCGGATTTAGCCGTGAAAAATGTGGATGTTTACCACGAAGAATTTGAAGCGCAAGCGGAAGTGCTAACGGATAAAGTGCTTGAACTTGAACAAAAAATGTCTATTTCCGAAGCGGCAAAAACGCAATTCGACAAAGCCTATCAGTTGGTTTGCAAAATTGCCGGCg                                                                                                                                                                                                                                                >  1:351450/1‑251 (MQ=255) ggCGTTGGATGCGCAACAAACCCGCGCATTGCAATATCAACAAGCCATTCAAGCCTTAGAAAAAGCCAAAACGTTGTGCGGTTTGGCGGATTTAGCCGTGAAAAATGTGGATGTTTACCACGAAGAATTTGAAGCGCAAGCGGAAGTGCTAACGGATAAAGTGCTTGAACTTGAACAAAAAATGTCTATTTCCGAAGCGGCAAAAACGCAATTCGACAAAGCCTATCAGTTGGTTTGCAAAATTGCCGGCg                                                                                                                                                                                                                                                >  1:766644/1‑251 (MQ=255)           gcgcAACAAACCCGCGCATTGCAATATCAACAAGCCATTCAAGCCTTAGAAAAAGCCAAAACGTTGTGCGGTTTGGCGGATTTAGCCGTGAAAAATGTGGATGTTTACCACGAAGAATTTGAAGCGCAAGCGGAAGTGCTAACGGATAAAGTGCTTGAACTTGAACAAAAAATGTCTATTTCCGAAGCGGCAAAAACGCAATTCGACAAAGCCTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCg                                                                                                                                                                                                                                      >  1:740069/1‑251 (MQ=255)              caacaaACCCGCGCATTGCAATATCAACAAGCCATTCAAGCCTTAGAAAAAGCCAAAACGTTGTGCGGTTTGGCGGATTTAGCCGTGAAAAATGTGGATGTTTACCACGAAGAATTTGAAGCGCAAGCGGAAGTGCTAACGGATAAAGTGCTTGAACTTGAACAAAAAATGTCTATTTCCGAAGCGGCAAAAACGCAATTCGACAAAGCCTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCGtt                                                                                                                                                                                                                                    >  1:550744/1‑250 (MQ=255)              caacaaACCCGCGCATTGCAATATCAACAAGCCATTCAAGCCTTAGAAAAAGCCAAAACGTTGTGCGGTTTGGCGGATTTAGCCGTGAAAAATGTGGATGTTTACCACGAAGAATTTGAAGCGCAAGCGGAAGTGCTAACGGATAAAGTGCTTGAACTTGAACAAAAAATGTCTATTTCCGAAGCGGCAAAAACGCAATTCGACAAAGCCTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCGt                                                                                                                                                                                                                                     <  1:180397/249‑1 (MQ=255)                 caaACCCGCGCATTGCAATATCAACAAGCCATTCAAGCCTTAGAAAAAGCCAAAACGTTGTGCGGTTTGGCGGATTTAGCCGTGAAAAATGTGGATGTTTACCACGAAGAATTTGAAGCGCAAGCGGAAGTGCTAACGGATAAAGTGCTTGAACTTGAACAAAAAATGTCTATTTCCGAAGCGGCAAAAACGCAATTCGACAAAGCCTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCCCGTTctgc                                                                                                                                                                                                                                <  1:774689/251‑1 (MQ=255)                           cATTGCAATATCAACAAGCCATTCAAGCCTTAGAAAAAGCCAAAACGTTGTGCGGTTTGGCGGATTTAGCCGTGAAAAATGTGGATGTTTACCACGAAGAATTTGAAGCGCAAGCGGAAGTGCTAACGGATAAAGTGCTTGAACTTGAACAAAAAATGTCTATTTCCGAAGCGGCAAAAACGCAATTCGACAAAGCCTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCGTTCTGCTGCGTGGGaa                                                                                                                                                                                                                      <  1:648959/251‑1 (MQ=255)                             ttGCAATATCAACAAGCCATTCAAGCCTTAGAAAAAGCCAAAACGTTGTGCGGTTTGGCGGATTTAGCCGTGAAAAATGTGGATGTTTACCACGAAGAATTTGAAGCGCAAGCGGAAGTGCTAACGGATAAAGTGCTTGAACTTGAACAAAAAATGTCTATTTCCGAAGCGGCAAAAACGCAATTCGACAAAGCCTATCAGTTGGTTTGCAAAATTGCTGGCGATGTACCTCGTTCTGCTGCGTGGGAAAg                                                                                                                                                                                                                    >  1:557722/1‑251 (MQ=255)                             ttGCAATATCAACAAGCCATTCAAGCCTTAGAAAAAGCCAAAACGTTGTGCGGTTTGGCGGATTTAGCCGTGAAAAATGTGGATGTTTACCACGAAGAATTTGAAGCGCAAGCGGAAGTGCTAACGGATAAAGTGCTTGAACTTGAACAAAAAATGTCTATTTCCGAAGCGGCAAAAACGCAATTCGACAAAGCCTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCg                                                                                                                                                                                                                                      >  1:752053/1‑233 (MQ=255)                             ttGCAATATCAACAAGCCATTCAAGCCTTAGAAAAAGCCAAAACGTTGTGCGGTTTGGCGGATTTAGCCGTGAAAAATGTGGATGTTTACCACGAAGAATTTGAAGCGCAAGCGGAAGTGCTAACGGATAAAGTGCTTGAACTTGAACAAAAAATGTCTATTTCCGAAGCGGCAAAAACGCAATTCGACAAAGCCTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCGTTCTGCTGCGt                                                                                                                                                                                                                           <  1:243871/244‑1 (MQ=255)                             ttGCAATATCAACAAGCCATTCAAGCCTTAGAAAAAGCCAAAACGTTGTGCGGTTTGGCGGATTTAGCCGTGAAAAATGTGGATGTTTACCACGAAGAATTTGAAGCGCAAGCGGAAGTGCTAACGGATAAAGTGCTTGAACTTGAACAAAAAATGTCTATTTCCGAAGCGGCAAAAACGCAATTCGACAAAGCCTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCGTTCTGCTGCGTGGGAAAg                                                                                                                                                                                                                    <  1:243254/251‑1 (MQ=255)                                cAATATCAACAAGCCATTCAAGCCTTAGAAAAAGCCAAAACGTTGTGCGGTTTGGCGGATTTAGCCGTGAAAAATGTGGATGTTTACCACGAAGAATTTGAAGCGCAAGCGGAAGTGCTAACGGATAAAGTGCTTGAACTTGAACAAAAAATGTCTATTTCCGAAGCGGCAAAAACGCAATTCGACAAAGCCTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCGTTCTGCTGCGTGGGAAAGTGc                                                                                                                                                                                                                 <  1:26999/251‑1 (MQ=255)                                 aaTATCAACAAGCCATTCAAGCCTTAGAAAAAGCCAAAACGTTGTGCGGTTTGGCGGATTTAGCCGTGAAAAATGTGGATGTTTACCACGAAGAATTTGAAGCGCAAGCGGAAGTGCTAACGGATAAAGTGCTTGAACTTGAACAAAAAATGTCTATTTCCGAAGCGGCAAAAACGCAATTCGACAAAGCCTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCGTTCTGCTGCGTGGGAAAGTGCg                                                                                                                                                                                                                <  1:777085/251‑1 (MQ=255)                                  atatCAACAAGCCATTCAAGCCTTAGAAAAAGCCAAAACGTTGTGCGGTTTGGCGGATTTAGCCGTGAAAAATGTGGATGTTTACCACGAAGAATTTGAAGCGCAAGCGGAAGTGCTAACGGATAAAGTGCTTGAACTTGAACAAAAAATGTCTATTTCCGAAGCGGCAAAAACGCAATTCGACAAAGCCTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCGTTctgc                                                                                                                                                                                                                                >  1:52555/1‑234 (MQ=255)                                  atatCAACAAGCCATTCAAGCCTTAGAAAAAGCCAAAACGTTGTGCGGTTTGGCGGATTTAGCCGTGAAAAATGTGGATGTTTACCACGAAGAATTTGAAGCGCAAGCGGAAGTGCTAACGGATAAAGTGCTTGAACTTGAACAAAAAATGTCTATTTCCGAAGCGGCAAAAACGCAATTCGACAAAGCCTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCGTTCTGCTGCGTGGGaa                                                                                                                                                                                                                      <  1:754566/244‑1 (MQ=255)                                  atatCAACAAGCCATTCAAGCCTTAGAAAAAGCCAAAACGTTGTGCGGTTTGGCGGATTTAGCCGTGAAAAATGTGGATGTTTACCACGAAGAATTTGAAGCGCAAGCGGAAGTGCTAACGGATAAAGTGCTTGAACTTGAACAAAAAATGTCTATTTCCGAAGCGGCAAAAACGCAATTCGACAAAGCCTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCGTTCTGCTGCGTGGGAAAGTGCGa                                                                                                                                                                                                               >  1:645814/1‑251 (MQ=255)                                    atCAACAAGCCATTCAAGCCTTAGAAAAAGCCAAAACGTTGTGCGGTTTGGCGGATTTAGCCGTGAAAAATGTGGATGTTTACCACGAAGAATTTGAAGCGCAAGCGGAAGTGCTAACGGATAAAGTGCTTGAACTTGAACAAAAAATGTCTATTTCCGAAGCGGCAAACACGCAATTCGACAAAGCCTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCGTTctgc                                                                                                                                                                                                                                >  1:443971/1‑232 (MQ=255)                                      caacaaGCCATTCAAGCCTTAGAAAAAGCCAAAACGTTGTGCGGTTTGGCGGATTTAGCCGTGAAAAATGTGGATGTTTACCACGAAGAATTTGAAGCGCAAGCGGAAGTGCTAACGGATAAAGTGCTTGAACTTGAACAAAAAATGTCTATTTCCGAAGCGGCAAAAACGCAATTCGACAAAGCCTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCGTTctgctg                                                                                                                                                                                                                              >  1:525389/1‑232 (MQ=255)                                             ccATTCAAGCCTTAGAAAAAGCCAAAACGTTGTGCGGTTTGGCGGATTTAGCCGTGAAAAATGTGGATGTTTACCACGAAGAATTTGAAGCGCAAGCGGAAGTGCTAACGGATAAAGTGCTTGAACTTGAACAAAAAATGTCTATTTCCGAAGCGGCAAAAACGCAATTCGACAAAGCCTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCGTTCTGCTGCGTGGGAAAg                                                                                                                                                                                                                    >  1:213784/1‑235 (MQ=255)                                               aTTCAAGCCTTAGAAAAAGCCAAAACGTTGTGCGGTTTGGCGGATTTAGCCGTGAAAAATGTGGATGTTTACCACGAAGAATTTGAAGCGCAAGCGGAAGTGCTAACGGATAAAGTGCTTGAACTTGAACAAAAAATGTCTATTTCCGAAGCGGCAAAAACGCAATTCGACAAAGCCTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCGTTCTGCTGCGt                                                                                                                                                                                                                           <  1:679029/226‑1 (MQ=255)                                               aTTCAAGCCTTAGAAAAAGCCAAAACGTTGTGCGGTTTGGCGGATTTAGCCGTGAAAAATGTGGATGTTTACCACGAAGAATTTGAAGCGCAAGCGGAAGTGCTAACGGATAAAGTGCTTGAACTTGAACAAAAAATGTCTATTTCCGAAGCGGCAAAAACGCAATTCGACAAAGCCTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCGTTCTGCTGCGTGGGAAAGTGCGAAAGAATTGTTGCg                                                                                                                                                                                                  >  1:747579/1‑251 (MQ=255)                                                   aaGCCTTAGAAAAAGCCAAAACGTTGTGCGGTTTGGCGGATTTAGCCGTGAAAAATGTGGATGTTTACCACGAAGAATTTGAAGCGCAAGCGGAAGTGCTAACGGATAAAGTGCTTGAACTTGAACAAAAAATGTCTATTTCCGAAGCGGCAAAAACGCAATTCGACAAAGCCTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCGTTCTGCTGCGTGGGAAAGTGCGAAAGAATTGTTGCGTGaa                                                                                                                                                                                              <  1:364428/251‑1 (MQ=255)                                                     gCCTTAGAAAAAGCCAAAACGTTGTGCGGTTTGGCGGATTTAGCCGTGAAAAATGTGGATGTTTACCACGAAGAATTTGAAGCGCAAGCGGAAGTGCTAACGGATAAAGTGCTTGAACTTGATCAAAAAATGTCTATTTCCGAAGCGGCAAAAACGCAATTCGACAAAGCCTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCGt                                                                                                                                                                                                                                     <  1:408172/210‑1 (MQ=255)                                                      ccTTAGAAAAAGCCAAAACGTTGTGCGGTTTGGCGGATTTAGCCGTGAAAAATGTGGATGTTTACCACGAAGAATTTGAAGCGCAAGCGGAAGTGCTAACGGATAAAGTGCTTGAACTTGAACAAAAAATGTCTATTTCCGAAGCGGCAAAAACGCAATTCGACAAAGCCTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTc                                                                                                                                                                                                                                       <  1:254824/207‑1 (MQ=255)                                                          aGAAAAAGCCAAAACGTTGTGCGGTTTGGCGGATTTAGCCGTGAAAAATGTGGATGTTTACCACGAAGAATTTGAAGCGCAAGCGGAAGTGCTAACGGATAAAGTGCTTGAACTTGAACAAAAAATGTCTATTTCCGAAGCGGCAAAAACGCAATTCGACAAAGCCTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCGTTCTGCTGCGTGGGAAAGTGCGAAAGAATTGTTGCGTGAATATCCGa                                                                                                                                                                                       >  1:617881/1‑251 (MQ=255)                                                                 gCCAAAACGTTGTGCGGTTTGGCGGATTTAGCCGTGAAAAATGTGGATGTTTACCACGAAGAATTTGAAGCGCAAGCGGAAGTGCTAACGGATAAAGTGCTTGAACTTGAACAAAAAATGTCTATTTCCGAAGCGGCAAAAACGCAATTCGACAAAGCCTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCGTTCTGCTGCGTGGGAAAGTGCGAAAGAATTGTTGCGTGAATATCCGACACaaaa                                                                                                                                                                                >  1:380101/1‑251 (MQ=255)                                                                  ccAAAACGTTGTGCGGTTTGGCGGATTTAGCCGTGAAAAATGTGGATGTTTACCACGAAGAATTTGAAGCGCAAGCGGAAGTGCTAACGGATAAAGTGCTTGAACTTGAACAAAAAATGTCTATTTCCGAAGCGGCAAAAACGCAATTCGACAAAGCCTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCGTTCTGCTGCGTGGGAAAGTGCGAAAGAATTGTTGCGTGAATATCCGACACaaaaa                                                                                                                                                                               >  1:145902/1‑251 (MQ=255)                                                                   cAAAACGTTGTGCGGTTTGGCGGATTTAGCCGTGAAAAATGTGGATGTTTACCACGAAGAATTTGAAGCGCAAGCGGAAGTGCTAACGGATAAAGTGCTTGAACTTGAACAAAAAATGTCTATTTCCGAAGCGGCAAAAACGCAATTCGACAAAGCCTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCGTTCTGCTGCGTGGGAAAGTGCGAAAGAATTGTTGCGTGAATATCCGACACAAAAAc                                                                                                                                                                              <  1:256055/251‑1 (MQ=255)                                                                     aaaCGTTGTGCGGTTTGGCGGATTTAGCCGTGAAAAATGTGGATGTTTACCACGAAGAATTTGAAGCGCAAGCGGAAGTGCTAACGGATAAAGTGCTTGAACTTGAACAAAAAATGTCTATTTCCGAAGCGGCAAAAACGCAATTCGACAAAGCCTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCGTTCTGCTGCGTGGGAAAGTGCGAAAGAATTGTTGCGTGAATATCCGACACAAAAACTg                                                                                                                                                                            <  1:808269/251‑1 (MQ=255)                                                                       aCGTTGTGCGGTTTGGCGGATTTAGCCGTGAAAAATGTGGATGTTTACCACGAAGAATTTGAAGCGCAAGCGGAAGTGCTAACGGATAAAGTGCTTGAACTTGAACAAAAAATGTCTATTTCCGAAGCGGCAAAAACGCAATTCGACAAAGCCTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCGTTCTGCTGCGTGGGAAAGTGCGAAAGAATTGTTGCGTGAATATCCGACACAAAAACTGCa                                                                                                                                                                          >  1:572727/1‑251 (MQ=255)                                                                            gtgCGGTTTGGCGGATTTAGCCGTGAAAAATGTGGATGTTTACCACGAAGAATTTGAAGCGCAAGCGGAAGTGCTAACGGATAAAGTGCTTGAACTTGAACAAAAAATGTCTATTTCCGAAGCGGCAAAAACGCAATTCGACAAAGCCTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCGTTCTGCTGCGTGGGAAAGTGCGAAAGAATTGTTGCGTGAATATCCGACACAAAAACTGCAAGca                                                                                                                                                                      >  1:694394/1‑250 (MQ=255)                                                                             tgCGGTTTGGCGGATTTAGCCGTGAAAAATGTGGATGTTTACCACGAAGAATTTGAAGCGCAAGCGGAAGTGCTAACGGATAAAGTGCTTGAACTTGAACAAAAAATGTCTATTTCCGAAGCGGCAAAAACGCAATTCGACAAAGCCTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCGTTCTGCTGCGTGGGAAAGTGCGAAAGAATTGTTGCGTGAATATCCGACACAAAAACTGCAAGcac                                                                                                                                                                     <  1:875943/250‑1 (MQ=255)                                                                               cGGTTTGGCGGATTTAGCCGTGAAAAATGTGGATGTTTACCACGAAGAATTTGAAGCGCAAGCGGAAGTGCTAACGGATAAAGTGCTTGAACTTGAACAAAAAATGTCTATTTCCGAAGCGGCAAAAACGCAATTCGACAAAGCCTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCGTTCTGCTGCGTGGGAAAGTGCGAAAGAATTGTTGCGTGAATATCCGACACAAAAACTGCAAGCACAGc                                                                                                                                                                  <  1:354698/251‑1 (MQ=255)                                                                                 gTTTGGCGGATTTAGCCGTGAAAAATGTGGATGTTTACCACGAAGAATTTGAAGCGCAAGCGGAAGTGCTAACGGATAAAGTGCTTGAACTTGAACAAAAAATGTCTATTTCCGAAGCGGCAAAAACGCAATTCGACAAAGCCTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCGTTCTGCTGCGTGGGAAAGTGCGAAAGAATTGTTGCGTGAATATCCGACACAAAAACTGCAAGCACAGCaa                                                                                                                                                                >  1:708632/1‑251 (MQ=255)                                                                                      gCGGATTTAGCCGTGAAAAATGTGGATGTTTACCACGAAGAATTTGAAGCGCAAGCGGAAGTGCTAACGGATAAAGTGCTTGAACTTGAACAAAAAATGTCTATTTCCGAAGCGGCAAAAACGCAATTCGACAAAGCCTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCGTTCTGCTGCGTGGGAAAGTGCGAAAGAATTGTTGCGTGAATATCCGACACAAAAACTGCAAGCACAGCAAacac                                                                                                                                                            <  1:795697/250‑1 (MQ=255)                                                                                       cGGATTTAGCCGTGAAAAATGTGGATGTTTACCACGAAGAATTTGAAGCGCAAGCGGAAGTGCTAACGGATAAAGTGCTTGAACTTGAACAAAAAATGTCTATTTCCGAAGCGGCAAAAACGCAATTCGACAAAGCCTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCGTTCTGCTGCGTGGGAAAGTGCGAAAGAATTGTTGCGTGAATATCCGACACAAAAACTGCAAGCACAGCAAACACCg                                                                                                                                                          <  1:447362/251‑1 (MQ=255)                                                                                                    gAAAAATGTGGATGTTTACCACGAAGAATTTGAAGCGCAAGCGGAAGTGCTAACGGATAAAGTGCTTGAACTTGAACAAAAAATGTCTATTTCCGAAGCGGCAAAAACGCAATTCGACAAAGCCTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCGTTctgctg                                                                                                                                                                                                                              >  1:190323/1‑170 (MQ=255)                                                                                                       aaaTGTGGATGTTTACCACGAAGAATTTGAAGCGCAAGCGGAAGTGCTAACGGATAAAGTGCTTGAACTTGAACAAAAAATGTCTATTTCCGAAGCGGCAAAAACGCAATTCGACAAAGCCTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCGTTCTGCTGCGTGGGAAAGTGCGAAAGAATTGTTGCGTGAATATCCGACACAAAAACTGCAAGCACAGCAAACACCGCAACTACGCGCCAAGt                                                                                                                                          >  1:294924/1‑251 (MQ=255)                                                                                                         aTGTGGATGTTTACCACGAAGAATTTGAAGCGCAAGCGGAAGTGCTAACGGATAAAGTGCTTGAACTTGAACAAAAAATGTCTATTTCCGAAGCGGCAAAAACGCAATTCGACAAAGCCTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCGTTCTGCTGCGTGGGAAAGTGCGAAAGAATTGTTGCGTGAATATCCGACACAAAAACTGCAAGCACAGCAAacac                                                                                                                                                            >  1:616993/1‑231 (MQ=255)                                                                                                              gATGTTTACCACGAAGAATTTGAAGCGCAAGCGGAAGTGCTAACGGATAAAGTGCTTGAACTTGAACAAAAAATGTCTATTTCCGAAGCGGCAAAAACGCAATTCGACAAAGCCTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCGTTCTGCTGCGTGGGAAAATGCGAAAGAATTGTTGCGTGAATATCCGACACAAAAACTGCAAGCACAGCAAACACCGCAACTACGCGCCAAGTTACATGa                                                                                                                                   >  1:423697/1‑251 (MQ=255)                                                                                                                 gTTTACCACGAAGAATTTGAAGCGCAAGCGGAAGTGCTAACGGATAAAGTGCTTGAACTTGAACAAAAAATGTCTATTTCCGAAGCGGCAAAAACGCAATTCGACAAAGCCTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCGTTCTGCTGCGTGGGAAAGTGCGAAAg                                                                                                                                                                                                            <  1:178045/175‑1 (MQ=255)                                                                                                                 gTTTACCACGAAGAATTTGAAGCGCAAGCGGAAGTGCTAACGGATAAAGTGCTTGAACTTGAACAAAAAATGTCTATTTCCGAAGCGGCAAAAACGCAATTCGACAAAGCCTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCGTTCTGCTGCGTGGGAAAGTGCGAAAGAATTGTTGCGTGAATATCCGACACAAAAACTGCAAGCACAGCAAACACCGCAACTACGCGCCAAGTTACATGAAtt                                                                                                                                >  1:456911/1‑251 (MQ=255)                                                                                                                      ccACGAAGAATTTGAAGCGCAAGCGGAAGTGCTAACGGATAAAGTGCTTGAACTTGAACAAAAAATGTCTATTTCCGAAGCGGCAAAAACGCAATTCGACAAAGCCTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCGTTCTGCTGCGTGGGAAAGTGCGAAAGAATTGTTGCGTGAATATCCGACACAAAAACTGCAAGCACAGCAAACACCGCAACTACGCGCCAAGTTACATGAATTGGaac                                                                                                                           <  1:376833/251‑1 (MQ=255)                                                                                                                           aagaaTTTGAAGCGCAAGCGGAAGTGCTAACGGATAAAGTGCTTGAACTTGAACAAAAAATGTCTATTTCCGAAGCGGCAAAAACGCAATTCGACAAAGCCTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCGTTCTGCTGCGTGGGAAAGTGCGAAAGAATTGTTGCGTGAATATCCGACACAAAAACTGCAAGCACAGCAAACACCGCAACTACGCGCCAAGTTACATGAATTGGAACAACGt                                                                                                                      <  1:392303/251‑1 (MQ=255)                                                                                                                             gaaTTTGAAGCGCAAGCGGAAGTGCTAACGGATAAAGTGCTTGAACTTGAACAAAAAATGTCTATTTCCGAAGCGGCAAAAACGCAATTCGACAAAGCCTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCGTTCTGCTGCGTGGGAAAGTGCGAAAGAATTGTTGCGCGAATATCCGACACAAAAACTGCAAGCACAGCAAACACCGCAAc                                                                                                                                                      >  1:626944/1‑217 (MQ=255)                                                                                                                              aaTTTGAAGCGCAAGCGGAAGTGCTAACGGATAAAGTGCTTGAACTTGAACAAAAAATGTCTATTTCCGAAGCGGCAAAAACGCAATTCGACAAAGCCTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCGTTCTGCTGCGTGGGAAAGTGCGAAAGAATTGTTGCGTGAATATCCGACACAAAAACTGCAAGCACAGCAAACACCGCAACTACGCGCCAAGTTACATGAATTGGAACAACGt                                                                                                                      >  1:816631/1‑248 (MQ=255)                                                                                                                              aaTTTGAAGCGCAAGCGGAAGTGCTAACGGATAAAGTGCTTGAACTTGAACAAAAAATGTCTATTTCCGAAGCGGCAAAAACGCAATTCGACAAAGCCTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCGTTCTGCTGCGTGGGAAAGTGCGAAAGAATTGTTGCGTGAATATCCGACACAAAAACTGCAAGCACAGCAAACACCGCAACTACGCGCCAAGTTACATGAATTGGAACAACGTc                                                                                                                     <  1:580236/249‑1 (MQ=255)                                                                                                                                 ttGAAGCGCAAGCGGAAGTGCTAACGGATAAAGTGCTTGAACTTGAACAAAAAATGTCTATTTCCGAAGCGGCAAAAACGCAATTCGACAAAGCCTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCGTTCTGCTGCGTGGGAAAGTGCGAAAGAATTGTTGCGTGAATATCCGACACAAAAACTGCAAGCACAGCAAACACCGCAACTACGCGCCAAGTTACATGAATTGGAACAACGTCTGAAt                                                                                                                <  1:602296/251‑1 (MQ=255)                                                                                                                                 ttGAAGCGCAAGCGGAAGTGCTAACGGATAAAGTGCTTGAACTTGAACAAAAAATGTCTATTTCCGAAGCGGCAAAAACGCAATTCGACAAAGCCTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCGTTCTGCTGCGTGGGAAAGTGCGAAAGAATTGTTGCGTGAATATCCGACACAAAAACTGCAAGCACAGCAAACACCGCAACTACGCGCCAAGTTACATGAATTGGAACAACGTCTGAAt                                                                                                                <  1:85852/251‑1 (MQ=255)                                                                                                                                  tGAAGCGCAAGCGGAAGTGCTAACGGATAAAGTGCTTGAACTTGAACAAAAAATGTCTATTTCCGAAGCGGCAAAAACGCAATTCGACAAAGCCTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCGTTCTGCTGCGTGGGAAAGTGCGAAAGAATTGTTGCGTGAATATCCGACACAAAAACTGCAAGCACAGCAAACACCGCAACTACGCGCCAAGTTACATGAATTGGAACAACGTCTGAATc                                                                                                               <  1:430908/251‑1 (MQ=255)                                                                                                                                          aaGCGGAAGTGCTAACGGATAAAGTGCTTGAACTTGAACAAAAAATGTCTATTTCCGAAGCGGCAAAAACGCAATTCGACAAAGCCTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCGTTCTGCTGCGTGGGAAAGTGCGAAAGAATTGTTGCGTGAATATCCGACACAAAAACTGCAAGCACAGCAAACACCGCAACTACGCGCCAAGTTACATGAATTGGAACAACGTCTGAATcaacaacaa                                                                                                       <  1:24307/251‑1 (MQ=255)                                                                                                                                               ctttatccgttagcacttccgCTTGAACTTGAACAAAAAATGTCTATTTCCGAAGCGGCAAAAACGCAATTCGACAAAGCCTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCGTTCTGCTGCGTGGGAAAGTGCGAAAGAATTGTTGCGTGAATATCCGACACAAAAACTGCAAGCACAGCAAACACCGCAACTACGCGCCAAGTTACATGAATTGGAACAACGTCTGAATCAACAACAAAg                                                                                                     <  1:590835/228‑1 (MQ=255)                                                                                                                                                    gCTAACGGATAAAGTGCTTGAACTTGAACAAAAAATGTCTATTTCCGAAGCGGCAAAAACGCAATTCGACAAAGCCTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCGTTCTGCTGCGTGGGAAAGTGCGAAAGAATTGTTGCGTGAATATCCGACACAAAAACTGCAAGCACAGCAAACACCGCAACTACGCGCCAAGTTACATGAATTGGAACAACGTCTGAATCAACAACAAAg                                                                                                     <  1:783786/243‑1 (MQ=255)                                                                                                                                                         cGGATAAAGTGCTTGAACTTGAACAAAAAATGTCTATTTCCGAAGCGGCAAAAACGCAATTCGACAAAGCCTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCGt                                                                                                                                                                                                                                     >  1:863535/1‑110 (MQ=255)                                                                                                                                                         cGGATAAAGTGCTTGAACTTGAACAAAAAATGTCTATTTCCGAAGCGGCAAAAACGCAATTCGACAAAGCCTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCGTTCTGCTGCGTGGGAAAGTGCGAAAGAATTGTTGCGTGAATATCCGACACAAAAACTGCAAGCACAGCAAACACCGCAACTACGCGCCAAGTTACATGAATTGGaac                                                                                                                           >  1:388950/1‑216 (MQ=255)                                                                                                                                                         cGGATAAAGTGCTTGAACTTGAACAAAAAATGTCTATTTCCGAAGCGGCAAAAACGCAATTCGACAAAGCCTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCGTTCTGCTGCGTGGGAAAGTGCGAAAGAATTGTTGCGTGAATATCCGACACAAAAACTGCAAGCACAGCAAACACCGCAACTACGCGCCAAGTTACATGAATTGGaac                                                                                                                           >  1:541128/1‑216 (MQ=255)                                                                                                                                                                   gCTTGAACTTGAACAAAAAATGTCTATTTCCGAAGCGGCAAAAACGCAATTCGACAAAGCCTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCGTTCTGCTGCGTGGGAAAGTGCGAAAGAATTGTTGCGTGAATATCCGACACAAAAACTGCAAGCACAGCAAACACCGCAACTAcgc                                                                                                                                                 >  1:146407/1‑184 (MQ=255)                                                                                                                                                                              aacAAAAAATGTCTATTTCCGAAGCGGCAAAAACGCAATTCGACAAAGCCTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCGTTCTGCTGCGTGGGAAAGTGCGAAAGAATTGTTGCGTGAATATCCGACACAAAAACTGCAAGCACAGCAAACACCGCAACTACGCGCCAAGTTACATGAATTGGAACAACGTCTGAATCAACAACAAAGTGCGGTCAGATTATTAAACGATTTTAATCAAcg                                                                    >  1:761983/1‑250 (MQ=255)                                                                                                                                                                                  aaaaaTGTCTATTTCCGAAGCGGCAAAAACGCAATTCGACAAAGCCTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCGTTCTGCTGCGTGGGAAAGTGCGAAAGAATTGTTGCGTGAATATCCGACACAAAAACTGCAAGCACAGCAAACACCGCAACTACGCGCCAAGTTACATGAATTGGAACAACGTCTGAATCAACAACAAAGTGCGGTCAGATTATTAAACGATTTTAATCAACGCGCCa                                                               >  1:146356/1‑251 (MQ=255)                                                                                                                                                                                        gTCTATTTCCGAAGCGGCAAAAACGCAATTCGACAAAGCCTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCGTTCTGCTGCGTGGGAAAGTGCGAAAGAATTGTTGCGTGAATATCCGACACAAAAACTGCAAGCACAGCAAACACCGCAACTACGCGCCAAGTTACATGAATTGGAACAACGTCTGAATCAACAACAAAGTGCGGTCAGATTATTAAACGATTTTAATCAACGCGCCAATTTGt                                                         >  1:666656/1‑251 (MQ=255)                                                                                                                                                                                               tCCGAAGCGGCAAAAACGCAATTCGACAAAGCCTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCGTTCTGCTGCGTGGGAAAGTGCGAAAGAATTGTTGCGTGAATATCCGACACAAAAACTGCAAGCACAGCAAACACCGCAACTACGCGCCAAGTTACATGAATTGGAACAACGTCTGAATCAACAACAAAGTGCGGTCAGATTATTAAACGATTTTAATCAACGCGCCAATTTGTCTTTAGa                                                  <  1:56082/251‑1 (MQ=255)                                                                                                                                                                                                        gCAAAAACGCAATTCGACAAAGCCTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCGTTCTGCTGCGTGGGAAAGTGCGAAAGAATTGTTGCGTGAATATCCGACACAAAAACTGCAAGCACAGCAAACACCGCAACTACGCGCCAAGTTACATGAATTGGAACAACGTCTGAATCAACAACAAAGTGCGGTCAg                                                                                            >  1:140309/1‑200 (MQ=255)                                                                                                                                                                                                              aCGCAATTCGACAAAGCCTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCGTTCTGCTGCGTGGGAAAGTGCGAAAGAATTGTTGCGTGAATATCCGACACAAAAACTGCAAGCACAGCAAACACCGCAACTACGCGCCAAGTTACATGAATTGGAACAACGTCTGAATCAACAACAAAGTGCGGTCAGATTATTAAACGATTTTAATCAACGCGCCAATTTGTCTTTAGAAACCGCAGATGAAtt                                   >  1:490265/1‑251 (MQ=255)                                                                                                                                                                                                                             gCCTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCGTTCTGCTGCGTGGGAAAGTGCGAAAGAATTGTTGCGTGAATATCCGACACAAAAACTGCAAGCACAGCAAACACCGCAACTACGCGCCAAGTTACATGAATTGGAACAACGTCTGAATCAACAACAAAGTGCGGTCAGATTATTAAACGATTTTAATCAACGCGCCAATTTGTCTTTAGAAACCGCAGATGAATTAGAAGAATTTTATgg                    >  1:662999/1‑251 (MQ=255)                                                                                                                                                                                                                             gCCTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCGTTCTGCTGCGTGGGAAAGTGCGAAAGAATTGTTGCGTGAATATCCGACACAAAAACTGCAAGCACAGCAAACACCGCAACTACGCGCCAAGTTACATGAATTGGAACAACGTCTGAATCAACAACAAAGTGCGGTCAGATTATTAAACGATTTTAATCAACGCGCCAATTTGTCTTTAGAAACCGCAGATGAATTAGAAGAATTTTATgg                    >  1:38629/1‑251 (MQ=255)                                                                                                                                                                                                                             gCCTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCGTTCTGCTGCGTGGGAAAGTGCGAAAGAATTGTTGCGTGAATATCCGACACAAAAACTGCAAGCACAGCAAACACCGCAACTACGCGCCAAGTTACATGAATTGGAACAACGTCTGAATCAACAACAAAATGCGGTCAGATTATTaa                                                                                    >  1:430076/1‑187 (MQ=255)                                                                                                                                                                                                                              ccTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCGTTCTGCTGCGTGGGAAAGTGCGAAAGAATTGTTGCGTGAATATCCGACACAAAAACTGCAAGCACAGCAAACACCGCAACTACGCGCCAAGTTACATGAATTGGAACAACGTCTGAATCAACAACAAAGTGCGGTCAGATTATTAAACGATTTTAATCAACGCGCCAATTTGTCTTTAGAAACCGCAGATGAATTAGAAGAATTTTATgg                    <  1:710744/250‑1 (MQ=255)                                                                                                                                                                                                                              ccTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCGTTCTGCTGCGTGGGAAAGTGCGAAAGAATTGTTGCGTGAATATCCGACACAAAAACTGCAAGCACAGCAAACACCGCAACTACGCGCCAAGTTACATGAATTGGAACAACGTCTGAATCAACAACAAAGTGCGGTCAGATTATTAAACGATTTTAATCAACGCGCCAATTTGTCTTTAGAAACCGCAGATGAATTAGAAGAATTTTATgg                    <  1:620856/250‑1 (MQ=255)                                                                                                                                                                                                                              ccTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCGTTCTGCTGCGTGGGAAAGTGCGAAAGAATTGTTGCGTGAATATCCGACACAAAAACTGCAAGCACAGCAAACACCGCAACTACGCGCCAAGTTACATGAATTGGAACAACGTCTGAATCAACAACAAAGTGCGGTCAGATTATTAAACGATTTTAATCAACGCGCCAATTTGTCTTTAGAAACCGCAGATGAATTAGAAGAATTTTATgg                    <  1:528381/250‑1 (MQ=255)                                                                                                                                                                                                                              ccTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCGTTCTGCTGCGTGGGAAAGTGCGAAAGAATTGTTGCGTGAATATCCGACACAAAAACTGCAAGCACAGCAAACACCGCAACTACGCGCCAAGTTACATGAATTGGAACAACGTCTGAATCAACAACAAAGTGCGGTCAGATTATTAAACGATTTTAATCAACGCGCCAATTTGTCTTTAGAAACCGCAGATGAATTAGAAGAATTTTATgg                    <  1:477895/250‑1 (MQ=255)                                                                                                                                                                                                                              ccTATCAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCGTTCTGCTGCGTGGGAAAGTGCGAAAGAATTGTTGCGTGAATATCCGACACAAAAACTGCAAGCACAGCAAACACCGCAACTACGCGCCAAGTTACATGAATTGGAACAACGTCTGAATCAACAACAAAGTGCGGTCAGATTATTAAACGATTTTAATCAACGCACCAATTTGTCTTTAGAAACCGCAGATGAATTAGAAGAATTTTATgg                    <  1:205658/250‑1 (MQ=255)                                                                                                                                                                                                                                   cAGTTGGTTTGCAAAATTGCCGGCGATGTACCTCGTTCTGCTGCGTGGGAAAGTGCGAAAGAATTGTTGCGTGAATATCCGACACAAAAACTGCAAGCACAGCAAACACCGCAACTACGCGCCAAGTTACATGAATTGGAACAACGTCTGAATCAACAACAAAGTGCGGTCAGATTATTAAACGATTTTAATc                                                                        >  1:751195/1‑193 (MQ=255)                                                                                                                                                                                                                                      ttGGTTTGCAAAATTGCCGGCGATGTACCTCGTTCTGCTGCGTGGGAAAGTGCGAAAGAATTGTTGCGTGAATATCCGACACAAAAACTGCAAGCACAGCAAACACCGCAACTACGCGCCAAGTTACATGAATTGGAACAACGTCTGAATCAACAACAAAGTGCGGTCAGATTATTAAACGATTTTAATCAACGCGCCAATTTGTCTTTAGAAACCGCAGATGAATTAGAAGAATTTTATGGTGAACAg             <  1:417899/249‑1 (MQ=255)                                                                                                                                                                                                                                        ggTTTGCAAAATTGCCGGCGATGTACCTCGTTCTGCTGCGTGGGAAAGTGCGAAAGAATTGTTGCGTGAATATCCGACACAAAAACTGCAAGCACAGCAAACACCGCAACTACGCGCCAAGTTACATGAATTGGAACAACGTCTGAATCAACAACAAAGTGCGGTCAGATTATTAAACGATTTTAATCAACGCGCCAATTTGTCTTTAGAAACCGCAGATGAATTAGAAGAATTTTATGGTGAACAGGAAg         >  1:708587/1‑251 (MQ=255)                                                                                                                                                                                                                                               aaaaTTGCCGGCGATGTACCTCGTTCTGCTGCGTGGGAAAGTGCGAAAGAATTGTTGCGTGAATATCCGACACAAAAACTGCAAGCACAGCAAACACCGCAACTACGCGCCAAGTTACATGAATTGGAACAACGTCTGAATCAACAACAAAGTGCGGTCAGATTATTAAACGATTTTAATCAACGCGCCAATTTGTCTTTAGAAACCGCAGATGAATTAGAAGAATTTTATGGTGAACAGGAAGCTTTAAt  <  1:256802/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    GGCGTTGGATGCGCAACAAACCCGCGCATTGCAATATCAACAAGCCATTCAAGCCTTAGAAAAAGCCAAAACGTTGTGCGGTTTGGCGGATTTAGCCGTGAAAAATGTGGATGTTTACCACGAAGAATTTGAAGCGCAAGCGGAAGTGCTAACGGATAAAGTGCTTGAACTTGAACAAAAAATGTCTATTTCCGAAGCGGCAAAAACGCAATTCGACAAAGCCTATCAGTTGGTTTGCAAAATTGTCGGCGATGTACCTCGTTCTGCTGCGTGGGAAAGTGCGAAAGAATTGTTGCGTGAATATCCGACACAAAAACTGCAAGCACAGCAAACACCGCAACTACGCGCCAAGTTACATGAATTGGAACAACGTCTGAATCAACAACAAAGTGCGGTCAGATTATTAAACGATTTTAATCAACGCGCCAATTTGTCTTTAGAAACCGCAGATGAATTAGAAGAATTTTATGGTGAACAGGAAGCTTTAAT  >  1/1285490‑1285978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑