Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	167,633	C→T	intergenic (‑32182/+46064)	unknown ← / ← unknown	SSU rRNA ## 16S rRNA, small subunit ribosomal RNA/tRNA‑Arg‑ACG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	167,633	0	C	T	100.0%	213.1 / NA	71	intergenic (‑32182/+46064)	unknown/unknown	SSU rRNA ## 16S rRNA, small subunit ribosomal RNA/tRNA‑Arg‑ACG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (40/31);  total (40/31)

CCCTTATTACCACAAGGCAGCTTACAAAATAGCGGCGATCGTGGCGAATCCAGCGCAACGGTGCGAGAAAAAGTCCTGAAAACCCGTGCCATTCAACTTCAGCGCGCCGGCAAAATTAATGCCCATTTAAATAGCAAAGAAATCGAACGGGACTGCAAACTAACCGACAAAGACGCACAGTTCCTGGAAAATGCCCTGACTAAACTGGGGCTTTCCGTACGAGCGTACCACCGCATACTGAAAGTGCCCCGCACCATTGCCGATTTAGATGGAGAACAACACATCAACCAGCGCCATCTGGCCGAAGCTCTGGGTTATCGGGCAATGGACAGGTTGTTGCAAAAATTGGCGAAAAACTCGGCGTAATGCGCAAAATTTTAAGGCACACCACGCAGTAAGGGCACATTTTCATAAGGTGGGACGGAAGTCGGTGCAGCGGCTTCACTAAATAAGAAAATAAACGGTTTCGGCGGAATAACCTTTTCATTACGC  >  1/167387‑167878                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      cccTTATTACCACAAGGCAGCTTACAAAATAGCGGCGATCGTGGCGAATCCAGCGCAACGGTGCGAGAAAAAGTCCTGAAAACCCGTGCCATTCAACTTCAGCGCGCCGGCAAAATTAATGCCCATTTAAATAGCAAAGAAATCGAACGGGACTGCAAACTAACCGACAAAGACGCACAGTTCCTGGAAAATGCCCTGACTAAACTGGGGCTTTCCGTACGAGCGTACCACCGCATACTGAAAGTGTCCCg                                                                                                                                                                                                                                                   >  1:652999/1‑251 (MQ=255)  ccTTATTACCACAAGGCAGCTTACAAAATAGCGGCGATCGTGGCGAATCCAGCGCAACGGTGCGAGAAAAAGTCCTGAAAACCCGTGCCATTCAACTTCAGCGCGCCGGCAAAATTAATGCCCATTTAAATAGCAAAGAAATCGAACGGGACTGCAAACTAACCGACAAAGACGCACAGTTCCTGGAAAATGCCCTGACTAAACTGGGGCTTTCCGTACGAGCGTACCACCGCATACTGAAAGTGTCCCg                                                                                                                                                                                                                                                   >  1:511486/1‑250 (MQ=255)    ttattaCCACAAGGCAGCTTACAAAATAGCGGCGATCGTGGCGAATCCAGCGCAACGGTGCGAGAAAAAGTCCTGAAAACCCGTGCCATTCAACTTCAGCGCGCCGGCAAAATTAATGCCCATTTAAATAGCAAAGAAATCGAACGGGACTGCAAACTAACCGACAAAGACGCACAGTTCCTGGAAAATGCCCTGACTAAACTGGGGCTTTCCGTACGAGCGTACCACCGCATACTGAAAGTGTCCCGCAc                                                                                                                                                                                                                                                <  1:226036/251‑1 (MQ=255)     tattaCCACAAGGCAGCTTACAAAATAGCGGCGATCGTGGCGAATCCAGCGCAACGGTGCGAGAAAAAGTCCTGAAAACCCGTGCCATTCAACTTCAGCGCGCCGGCAAAATTAATGCCCATTTAAATAGCAAAGAAATCGAACGGGACTGCAAACTAACCGACAAAGACGCACAGTTCCTGGAAAATGCCCTGACTAAACTGGGGCTTTCCGTACGAGCGTACCACCGCATACTGAAAGTGTCCCGCAc                                                                                                                                                                                                                                                >  1:531005/1‑250 (MQ=255)          ccACAAGGCAGCTTACAAAATAGCGGCGATCGTGGCGAATCCAGCGCAACGGTGCGAGAAAAAGTCCTGAAAACCCGTGCCATTCAACTTCAGCGCGCCGGCAAAATTAATGCCCATTTAAATAGCAAAGAAATCGAACGGGACTGCAAACTAACCGACAAAGACGCACAGTTCCTGGAAAATGCCCTGACTAAACTGGGGCTTTCCGTACGAGCGTACCACCGCATACTGAAAGTGTCCCGCACCATTg                                                                                                                                                                                                                                           >  1:650045/1‑250 (MQ=255)             caAGGCAGCTTACAAAATAGCGGCGATCGTGGCGAATCCAGCGCAACGGTGCGAGAAAAAGTCCTGAAAACCCGTGCCATTCAACTTCAGCGCGCCGGCAAAATTAATGCCCATTTAAATAGCAAAGAAATCGAACGGGACTGCAAACTAACCGACAAAGACGCACAGTTCCTGGAAAATGCCCTGACTAAACTGGGGCTTTCCGTACGAGCGTACCACCGCATACTGAAAGTGTCCCGCACCATTGCCGa                                                                                                                                                                                                                                       >  1:823791/1‑251 (MQ=255)                      ttACAAAATAGCGGCGATCGTGGCGAATCCAGCGCAACGGTGCGAGAAAAAGTCCTGAAAACCCGTGCCATTCAACTTCAGCGCGCCGGCAAAATTAATGCCCATTTAAATAGCAAAGAAATCGAACGGGACTGCAAACTAACCGACAAAGACGCACAGTTCCTGGAAAATGCCCTGACTAAACTGGGGCTTTCCGTACGAGCGTACCACCGCATACTGAAAGTGTCCCGCACCATTGCCGATTTAGATgg                                                                                                                                                                                                                              >  1:63807/1‑251 (MQ=255)                                                aTCCAGCGCAACGGTGCGAGAAAAAGTCCTGAAAACCCGTGCCATTCAACTTCAGCGCGCCGGCAAAATTAATGCCCATTTAAATAGCAAAGAAATCGAACGGGACTGCAAACTAACCGACAAAGACGCACAGTTCCTGGAAAATGCCCTGACTAAACTGGGGCTTTCCGTACGAGCGTACCACCGCATACTGAAAGTGTCCCGCACCATTGCCGATTTAGATGGAGAACAACACATCAAcc                                                                                                                                                                                                             <  1:467073/242‑1 (MQ=255)                                                aTCCAGCGCAACGGTGCGAGAAAAAGTCCTGAAAACCCGTGCCATTCAACTTCAGCGCGCCGGCAAAATTAATGCCCATTTAAATAGCAAAGAAATCGAACGGGACTGCAAACTAACCGACAAAGACGCACAGTTCCTGGAAAATGCCCTGACTAAACTGGGGCTTTCCGTACGAGCGTACCACCGCATACTGAAAGTGTCCCGCACCATTGCCGATTTAGATGGAGAACAACACATCAACCAgcgc                                                                                                                                                                                                        >  1:347670/1‑247 (MQ=255)                                                aTCCAGCGCAACGGTGCGAGAAAAAGTCCTGAAAACCCGTGCCATTCAACTTCAGCGCGCCGGCAAAATTAATGCCCATTTAAATAGCAAAGAAATCGAACGGGACTGCAAACTAACCGACAAAGACGCACAGTTCCTGGAAAATGCCCTGACTAAACTGGGGCTTTCCGTACGAGCGTACCACCGCATACTGAAAGTGTCCCGCACCATTGCCGATTTAGATGGAGAACAACACATCAACCAGCGCCAt                                                                                                                                                                                                     >  1:457884/1‑250 (MQ=255)                                                   cAGCGCAACGGTGCGAGAAAAAGTCCTGAAAACCCGTGCCATTCAACTTCAGCGCGCCGGCAAAATTAATGCCCATTTAAATAGCAAAGAAATCGAACGGGACTGCAAACTAACCGACAAAGACGCACAGTTCCTGGAAAATGCCCTGACTAAACTGGGGCTTTCCGTACGAGCGTACCACCGCATACTGAAAGTGTCCCGCACCATTGCCGATTTAGATGGAGAACAACACATCAACCAGCGCCATCTgg                                                                                                                                                                                                 >  1:733378/1‑251 (MQ=255)                                                             gTGCGAGAAAAAGTCCTGAAAACCCGTGCCATTCAACTTCAGCGCGCCGGCAAAATTAATGCCCATTTAAATAGCAAAGAAATCGAACGGGACTGCAAACTAACCGACAAAGACGCACAGTTCCTGGAAAATGCCCTGACTAAACTGGGGCTTTCCGTACGAGCGTACCACCGCATACTGAAAGTGTCCCGCAc                                                                                                                                                                                                                                                <  1:50379/194‑1 (MQ=255)                                                                           ccTGAAAACCCGTGCCATTCAACTTCAGCGCGCCGGCAAAATTAATGCCCATTTAAATAGCAAAGAAATCGAACGGGACTGCAAACTAACCGACAAAGACGCACAGTTCCTGGAAAATGCCCTGACTAAACTGGGGCTTTCCGTACGAGCGTACCACCGCATACTGAAAGTGTCCCGCACCATTGCCGATTTAGATGGAGAACAACACATCAAcc                                                                                                                                                                                                             >  1:880410/1‑215 (MQ=255)                                                                           ccTGAAAACCCGTGCCATTCAACTTCAGCGCGCCGGCAAAATTAATGCCCATTTAAATAGCAAAGAAATCGAACGGGACTGCAAACTAACCGACAAAGACGCACAGTTCCTGGAAAATGCCCTGACTAAACTGGGGCTTTCCGTACGAGCGTACCACCGCATACTGAAAGTGTCCCGCACCATTGCCGATTTAGATGGAGAACAACACATCAACCAGCGCCATCTGGCCGAAGCTCTGGGTTATCGGGCaa                                                                                                                                                                         >  1:87479/1‑251 (MQ=255)                                                                           ccTGAAAACCCGTGCCATTCAACTTCAGCGCGCCGGCAAAATTAATGCCCATTTAAATAGCAAAGAAATCGAACGGGACTGCAAACTAACCGACAAAGACGCACAGTTCCTGGAAAATGCCCTGACTAAACTGGGGCTTTCCGTACGAGCGTACCACCGCATACTGAAAGTGTCCCGCACCATTGCCGATTTAGATGGAGAACAACACATCAACCAGCGCCATCTGGCCGAAGCTCTGGGTTATCGGGCaa                                                                                                                                                                         >  1:237310/1‑251 (MQ=255)                                                                                aaaCCCGTGCCATTCAACTTCAGCGCGCCGGCAAAATTAATGCCCATTTAAATAGCAAAGAAATCGAACGGGACTGCAAACTAACCGACAAAGACGCACAGTTCCTGGAAAATGCCCTGACTAAACTGGGGCTTTCCGTACGAGCGTACCACCGCATACTGAAAGTGTCCCGCACCATTGCCGTTTTAGATGGAGAACAACACATCAACCAGCGCCATCTGGCCGAAGCTCTGGGTTATCGGGCAATGGAc                                                                                                                                                                    <  1:860705/251‑1 (MQ=255)                                                                                aaaCCCGTGCCATTCAACTTCAGCGCGCCGGCAAAATTAATGCCCATTTAAATAGCAAAGAAATCGAACGGGACTGCAAACTAACCGACAAAGACGCACAGTTCCTGGAAAATGCCCTGACTAAACTGGGGCTTTCCGTACGAGCGTACCACCGCATACTGAAAGTGTCCCGCACCATTGCCGATTTAGATGGAGAACAACACATCAACCAGCGCCATCTGGCCGAAGCTCTGGGTTATCGGGCAATGGAc                                                                                                                                                                    <  1:578989/251‑1 (MQ=255)                                                                                aaaCCCGTGCCATTCAACTTCAGCGCGCCGGCAAAATTAATGCCCATTTAAATAGCAAAGAAATCGAACGGGACTGCAAACTAACCGACAAAGACGCACAGTTCCTGGAAAATGCCCTGACTAAACTGGGGCTTTCCGTACGAGCGTACCACCGCATACTGAAAGTGTCCCGCACCATTGCCGATTTAGATGGAGAACAACACATCAACCAGCGCCATCTGGCCGAAGCTCTGGGTTATCGGGCAATGGAc                                                                                                                                                                    <  1:421450/251‑1 (MQ=255)                                                                                 aaCCCGTGCCATTCAACTTCAGCGCGCCGGCAAAATTAATGCCCATTTAAATAGCAAAGAAATCGAACGGGACTGCAAACTAACCGACAAAGACGCACAGTTCCTGGAAAATGCCCTGACTAAACTGGGGCTTTCCGTACGAGCGTACCACCGCATACTGAAAGTGTCCCGCACCATTGCCGATTTAGATGGAGAACAACACATCAACCAGCGCCATCTGGCCGAAGCTCTGGGTTATCGGGCAATGGACa                                                                                                                                                                   >  1:727997/1‑251 (MQ=255)                                                                                   cccGTGCCATTCAACTTCAGCGCGCCGGCAAAATTAATGCCCATTTAAATAGCAAAGAAATCGAACGGGACTGCAAACTAACCGACAAAGACGCACAGTTCCTGGAAAATGCCCTGACTAAACTGGGGCTTTCCGTACGAGCGTACCACCGCATACTGAAAGTGTCCCGCACCATTGCCGATTTAGATGGAGAACAACACATCAACCAGCGCCATCTGGCCGAAGCTCTGGGTTATCGGGCAATGGACAgg                                                                                                                                                                 <  1:216659/251‑1 (MQ=255)                                                                                           aTTCAACTTCAGCGCGCCGGCAAAATTAATGCCCATTTAAATAGCAAAGAAATCGAACGGGACTGCAAACTAACCGACAAAGACGCACAGTTCCTGGAAAATGCCCTGACTAAACTGGGGCTTTCCGTACGAGCGTACCACCGCATACTGAAAGTGTCCCGCACCATTGCCGATTTAGATGGAGAACAACACATCAACCAGCGCCATCTGGCCGAAGCTCTGGGTTATCGGGCAATGGACAGGTTGTTGCa                                                                                                                                                         >  1:693765/1‑251 (MQ=255)                                                                                            ttCAACTTCAGCGCGCCGGCAAAATTAATGCCCATTTAAATAGCAAAGAAATCGAACGGGACTGCAAACTAACCGACAAAGACGCACAGTTCCTGGAAAATGCCCTGACTAAACTGGGGCTTTCCGTACGAGCGTACCACCGCATACTGAAAGTGTCCCGCACCATTGCCGATTTAGATGGAGAACAACACATCAACCAGCGCCATCTGGCCGAAGCTCTGGGTTATCGGGCAATGGACAGGTTGTTGCaa                                                                                                                                                        >  1:371896/1‑251 (MQ=255)                                                                                               aaCTTCAGCGCGCCGGCAAAATTAATGCCCATTTAAATAGCAAAGAAATCGAACGGGACTGCAAACTAACCGACAAAGACGCACAGTTCCTGGAAAATGCCCTGACTAAACTGGGGCTTTCCGTACGAGCGTACCACCGCATACTGAAAGTGTCCCGCACCATTGCCGATTTAGATGGAGAACAACACATCAACCAGCGCCATCTGGCCGAAGCTCTGGGTTATCGGGCAATGGACAGGTTGTTGCaaaaa                                                                                                                                                     <  1:795102/251‑1 (MQ=255)                                                                                                aCTTCAGCGCGCCGGCAAAATTAATGCCCATTTAAATAGCAAAGAAATCGAACGGGACTGCAAACTAACCGACAAAGACGCACAGTTCCTGGAAAATGCCCTGACTAAACTGGGGCTTTCCGTACGAGCGTACCACCGCATACTGAAAGTGTCCCGCACCATTGCCGATTTAGATGGAGAACAACACATCAACCAGCGCCATCTGGCCGAAGCTCTGGGTTATCGGGCAATGGACAgg                                                                                                                                                                 >  1:280432/1‑238 (MQ=255)                                                                                                aCTTCAGCGCGCCGGCAAAATTAATGCCCATTTAAATAGCAAAGAAATCGAACGGGACTGCAAACTAACCGACAAAGACGCACAGTTCCTGGAAAATGCCCTGACTAAACTGGGGCTTTCCGTACGAGCGTACCACCGCATACTGAAAGTGTCCCGCACCATTGCCGATTTAGATGGAGAACAACACATCAACCAGCGCCATCTGGCCGAAGCTCTGGGTTATCGGGCAATGGACAGGTTGTTGCAAAAAt                                                                                                                                                    >  1:187091/1‑251 (MQ=255)                                                                                                                   aTTAATGCCCATTTAAATAGCAAAGAAATCGAACGGGACTGCAAACTAACCGACAAAGACGCACAGTTCCTGGAAAATGCCCTGACTAAACTGGGGCTTTCCGTACGAGCGTACCACCGCATACTGAAAGTGTCCCGCACCATTGCCGATTTAGATGGAGAACAACACATCAACCAGCGCCATCTGGCCGAAGCTCTGGGTTATCGGGCAATGGACAGGTTGTTGCAAAAATTGGCGAAAAACTCGGCGTa                                                                                                                                 >  1:601976/1‑251 (MQ=255)                                                                                                                   aTTAATGCCCATTTAAATAGCAAAGAAATCGAACGGGACTGCAAACTAACCGACAAAGACGCACAGTTCCTGGAAAATGCCCTGACTAAACTGGGGCTTTCCGTACGAGCGTACCACCGCATACTGAAAGTGTCCCGCACCATTGCCGATTTAGATGGAGAACAACACATCAACCAGCGCCATCTGGCCGAAGCTCTGGGTTATCGGGCAATGGACAGGTTGTTGCAAAAATTGGCGAAAAACTCGGCGTa                                                                                                                                 >  1:881550/1‑251 (MQ=255)                                                                                                                      aaTGCCCATTTAAATAGCAAAGAAATCGAACGGGACTGCAAACTAACCGACAAAGACGCACAGTTCCTGGAAAATGCCCTGACTAAACTGGGGCTTTCCGTACGAGCGTACCACCGCATACTGAAAGTGTCCCGCACCATTGCCGATTTAGATGGAGAACAACACATCAACCAGCGCCATCTGGCCGAAGCTCTGGGTTATCGGGCAATGGACAGGTTGTTGCAAAAATTGGCGAAAAACTCGGCGTAATg                                                                                                                              <  1:623658/251‑1 (MQ=255)                                                                                                                            cATTTAAATAGCAAAGAAATCGAACGGGACTGCAAACTAACCGACAAAGACGCACAGTTCCTGGAAAATGCCCTGACTAAACTGGGGCTTTCCGTACGAGCGTACCACCGCATACTGAAAGTGTCCCGCACCATTGCCGATTTAGATGGAGAACAACACATCAACCAGCGCCATCTGGc                                                                                                                                                                                                <  1:292136/179‑1 (MQ=255)                                                                                                                            cATTTAAATAGCAAAGAAATCGAACGGGACTGCAAACTAACCGACAAAGACGCACAGTTCCTGGAAAATGCCCTGACTAAACTGGGGCTTTCCGTACGAGCGTACCACCGCATACTGAAAGTGTCCCGCACCATTGCCGATTTAGATGGAGAACAACACATCAACCAGCGCCATCTGGCCGAAGCTCTGGGt                                                                                                                                                                                   >  1:604791/1‑192 (MQ=255)                                                                                                                                   aTAGCAAAGAAATCGAACGGGACTGCAAACTAACCGACAAAGACGCACAGTTCCTGGAAAATGCCCTGACTAAACTGGGGCTTTCCGTACGAGCGTACCACCGCATACTGAAAGTGTCCCGCACCATTGCCGATTTAGATGGAGAACAACACATCAACCAGCGCCATCTGGCCGAAGCTCTGGGTTATCGGGCAATGGACAGGTTGTTGCAAAAATTGGCGAAAAACTCGGCGTAATGCGCAAAATTTTaa                                                                                                                 <  1:281564/251‑1 (MQ=255)                                                                                                                                    tAGCAAAGAAATCGAACGGGACTGCAAACTAACCGACAAAGACGCACAGTTCCTGGAAAATGCCCTGACTAAACTGGGGCTTTCCGTACGAGCGTACCACCGCATACTGAAAGTGTCCCGCACCATTGCCGATTTAGATGGAGAACAACACATCAACCAGCGCCATCTGGCCGAAGCTCTGGGTTATCGGGCAATGGACAGGTTGTTGCAAAAATTGGCGAAAAACTCGGCGTAATGCGCAAAATTTTAAg                                                                                                                <  1:314362/251‑1 (MQ=255)                                                                                                                                             aaTCGAACGGGACTGCAAACTAACCGACAAAGACGCACAGTTCCTGGAAAATGCCCTGACTAAACTGGGGCTTTCCGTACGAGCGTACCACCGCATACTGAAAGTGTCCCGCACCATTGCCGATTTAGATGGAGAACAACACATCAACCAGCGCCATCTGGCCGAAGCTCTGGGTTATCGGGCAATGGACAGGTTGTTGCAAAAATTGGCGAAAAACTCGGCGTAATGCGCAAAATTTTAAGGCAcaccac                                                                                                       <  1:662913/251‑1 (MQ=255)                                                                                                                                                  aaCGGGACTGCAAACTAACCGACAAAGACGCACAGTTCCTGGAAAATGCCCTGACTAAACTGGGGCTTTCCGTACGAGCGTACCACCGCATACTGAAAGTGTCCCGCACCATTGCCGATTTAGATGGAGAACAACACATCAACCAGCGCCATCTGGCCGAAGCTCTGGGTTATCGGGCAATGGACAGGTTGTTGCAAAAATTGGCGAAAAACTCGGCGTAATGCGCAAAATTTTAAGGCACACCACGCAGt                                                                                                  <  1:67876/251‑1 (MQ=255)                                                                                                                                                  aaCGGGACTGCAAACTAACCGACAAAGACGCACAGTTCCTGGAAAATGCCCTGACTAAACTGGGGCTTTCCGTACGAGCGCACCACCGCATACTGAAAGTGTCCCGCACCATTGCCGATTTAGATGGAGAACAACACATCAACCAGCGCCATCTGGCCGAAGCTCTGGGTTATCGGGCAATGGACAGGTTGTTGCAAAAATTGGCGAAAAACTCGGCGTAATGCGCAAAATTTTAAGGCACACCACGCAg                                                                                                   <  1:434597/250‑1 (MQ=255)                                                                                                                                                      ggACTGCAAACTAACCGACAAAGACGCACAGTTCCTGGAAAATGCCCTGACTAAACTGGGGCTTTCCGTACGAGCGTACCACCGCATACTGAAAGTGTCCCGCACCATTGCCGATTTAGATGGAGAACAACACATCAACCAGCGCCATCTGGCCGAAGCTCTGGGTTATCGGGCAATGGACAGGTTGTTGCAAAAATTGGCGAAAAACTCGGCGTAATGCGCAAAATTTTAAGGCAcaccac                                                                                                       <  1:551971/242‑1 (MQ=255)                                                                                                                                                      ggACTGCAAACTAACCGACAAAGACGCACAGTTCCTGGAAAATGCCCTGACTAAACTGGGGCTTTCCGTACGAGCGTACCACCGCATACTGAAAGTGTCCCGCACCATTGCCGATTTAGATGGAGAACAACACATCAACCAGCGCCATCTGGCCGAAGCTCTGGGTTATCGGGCAATGGACAGGTTGTTGCAAAAATTGGCGAAAAACTCGGCGTAATGCGCAAAATTTTAAGGCACACCACGCAGTAAgg                                                                                              >  1:556684/1‑251 (MQ=255)                                                                                                                                                        aCTGCAAACTAACCGACAAAGACGCACAGTTCCTGGAAAATGCCCTGACTAAACTGGGGCTTTCCGTACGAGCGTACCACCGCATACTGAAAGTGTCCCGCACCATTGCCGATTTAGATGGAGAACAACACATCAACCAGCGCCATCTGGCCGAAGCTCTGGGTTATCGGGCAATGGACAGGTTGTTGCAAAAATTGGCGAAAAACTCGGCGTAATGCGCAAAATTTTAAGGCACACCACGCAGTAAggg                                                                                             <  1:199525/250‑1 (MQ=255)                                                                                                                                                        aCTGCAAACTAACCGACAAAGACGCACAGTTCCTGGAAAATGCCCTGACTAAACTGGGGCTTTCCGTACGAGCGTACCACCGCATACTGAAAGTGTCCCGCACCATTGCCGATTTAGATGGAGAACAACACATCAACCAGCGCCATCTGGCCGAAGCTCTGGGTTATCGGGCAATGGACAGGTTGTTGCAAAAATTGGCGAAAAACTCGGCGTAATGCGCAAAATTTTAAGGCACACCACGCAGTAAggg                                                                                             <  1:236919/250‑1 (MQ=255)                                                                                                                                                          tGCAAACTAACCGACAAAGACGCACAGTTCCTGGAAAATGCCCTGACTAAACTGGGGCTTTCCGTACGAGCGTACCACCGCATACTGAAAGTGTCCCGCACCATTGCCGATTTAGATGGAGAACAACACATCAACCAGCGCCATCTGGCCGAAGCTCTGGGTTATCGGGCAATGGACAGGTTGTTGCAAAAATTGGCGAAAAACTCGGCGTAATGCGCAAAATTTTAAGGCACACCACGCAGTAAGGGcac                                                                                          <  1:398036/251‑1 (MQ=255)                                                                                                                                                          tGCAAACTAACCGACAAAGACGCACAGTTCCTGGAAAATGCCCTGACTAAACTGGGGCTTTCCGTACGAGCGTACCACCGCATACTGAAAGTGTCCCGCACCATTGCCGATTTAGATGGAGAACAACACATCAACCAGCGCCATCTGGCCGAAGCTCTGGGTTATCGGGCAATGGACAGGTTGTTGCAAAAATTGGCGAAAAACTCGGCGTAATGCGCAAAATTTTAAGGCACACCACGCAGTAAGGGcac                                                                                          <  1:275637/251‑1 (MQ=255)                                                                                                                                                             aaaCTAACCGACAAAGACGCACAGTTCCTGGAAAATGCCCTGACTAAACTGGGGCTTTCCGTACGAGCGTACCACCGCATATTGAAAGTGTCCCGCACCATTGCCGATTTAGATGGAGAACAACACATCAACCAGCGCCATCTGGCCGAAGCTCTGGGTTATCGGGCAATGGACAGGTTGTTGCAAAAATTGGCGAAAAACTCGGCGTAATGCGCAAAATTTTAAGGCACACCACGCAGTAAGGGCACAtt                                                                                       <  1:434319/251‑1 (MQ=255)                                                                                                                                                                cTAACCGACAAAGACGCACAGTTCCTGGAAAATGCCCTGACTAAACTGGGGCTTTCCGTACGAGCGTACCACCGCATACTGAAAGTGTCCCGCACCATTGCCGATTTAGAt                                                                                                                                                                                                                                <  1:452237/111‑1 (MQ=255)                                                                                                                                                                  aaCCGACAAAGACGCACAGTTCCTGGAAAATGCCCTGACTAAACTGGGGCTTTCCGTACGAGCGTACCACCGCATACTGAAAGTGTCCCGCACCATTGCCGATTTAGATGGAGAACAACACATCAACCAGCGCCATCTGGCCGATGCTCTGGGTTATCGGGCAATGGACAGGTTGTTGCAAAAATTGGCGAAAAACTCGGCGTAATGCGCAAAATTTTAAGGCACACCACGCAGTAAGGGCACATTTTCAt                                                                                  >  1:780161/1‑251 (MQ=255)                                                                                                                                                                  aaCCGACAAAGACGCACAGTTCCTGGAAAATGCCCTGACTAAACTGGGGCTTTCCGTACGAGCGTACCACCGCATACTGAAAGTGTCCCGCACCATTGCCGATTTAGATGGAGAACAACACATCAACCAGCGCCATCTGGCCGAAGCTCTGGGTTATCGGGCAATGGACAGGTTGTTGCAAAAATTGGCGAAAAACTCGGCGTAATGCGCAAAATTTTAAGGCACACCACGc                                                                                                     <  1:743681/232‑1 (MQ=255)                                                                                                                                                                    ccGACAAAGACGCACAGTTCCTGGAAAATGCCCTGACTAAACTGGGGCTTTCCGTACGAGCGTACCACCGCATACTGAAAGTGTCCCGCACCATTGCCGATTTAGATGGAGAACAACACATCAACCAGCGCCATCTGGCCGAAGCTCTGGGTTATCGGGCAATGGACAGATTGTTGCAAAAATTGGCGAAAAACTCGGCGTAATGCGCAAAATTTTAAGGCACACCACGCAGTAAGGGCACAttt                                                                                      >  1:423582/1‑245 (MQ=255)                                                                                                                                                                          aaGACGCACAGTTCCTGGAAAATGCCCTGACTAAACTGGGGCTTTCCGTACGAGCGTACCACCGCATACTGAAAGTGTCCCGCACCATTGCCGATTTAGATGGAGaacaa                                                                                                                                                                                                                       <  1:589960/110‑1 (MQ=255)                                                                                                                                                                                    gTTCCTGGAAAATGCCCTGACTAAACTGGGGCTTTCCGTACGAGCGTACCACCGCATACTGAAAGTGTCCCGCACCATTGCCGATTTAGATGGAGAACAACACATCAACCAGCGCCATCTGGCCGAAGCTCTGGGTTATCGGGCAATGGAc                                                                                                                                                                    >  1:498658/1‑151 (MQ=255)                                                                                                                                                                                    gTTCCTGGAAAATGCCCTGACTAAACTGGGGCTTTCCGTACGAGCGTACCACCGCATACTGAAAGTGTCCCGCACCATTGCCGATTTAGATGGAGAACAACACATCAACCAGCGCCATCTGGCCGAAGCTCTGGGTTATCGGGCAATGGACAGGTTGTTGCAAAAATTGGCGAAAAACTCGGCGTAATGCGCAAAATTTTAAGGCACACCACGCAg                                                                                                   <  1:876170/216‑1 (MQ=255)                                                                                                                                                                                    gTTCCTGGAAAATGCCCTGACTAAACTGGGGCTTTCCGTACGAGCGTACCACCGCATACTGAAAGTGTCCCGCACCATTGCCGATTTAGATGGAGAACAACACATCAACCAGCGCCATCTGGCCGAAGCTCTGGGTTATCGGGCAATGGACAGGTTGTTGCAAAAATTGGCGAAAAACTCGGCGTAATGCGCAAAATTTTAAGGCACACCACGCAGt                                                                                                  >  1:170513/1‑217 (MQ=255)                                                                                                                                                                                    gTTCCTGGAAAATGCCCTGACTAAACTGGGGCTTTCCGTACGAGCGTACCACCGCATACTGAAAGTGTCCCGCACCATTGCCGATTTAGATGGAGAACAACACATCAACCAGCGCCATCTGGCCGAAGCTCTGGGTTATCGGGCAATGGACAGGTTGTTGCAAAAATTGGCGAAAAACTCGGCGTAATGCGCAAAATTTTAAGGCACACCACGCAGTAAGGGCACATTTTCATAAGGTGGGACGGAAGTCg                                                                >  1:166229/1‑251 (MQ=255)                                                                                                                                                                                    gTTCCTGGAAAATGCCCTGACTAAACTGGGGCTTTCCGTACGAGCGTACCACCGCATACTGAAAGTGTCCCGCACCATTGCCGATTTAGATGGAGAACAACACATCAACCAGCGCCATCTGGCCGAAGCTCTGGGTTATCGGGCAATGGACAGGTTGTTGCAAAAATTGGCGAAAAACTCGGCGTAATGCGCAAAATTTTAAGGCACACCACGCAGTAAGGGCACATTTTCATAAGGTGGGACGGAAGTCg                                                                >  1:704336/1‑251 (MQ=255)                                                                                                                                                                                    gTTCCTGGAAAATGCCCTGACTAAACTGGGGCTTTCCGTACGAGCGTACCACCGCATACTGAAAGTGTCCCGCACCATTGCCGATTTAGATGGAGAACAACACATCAACCAGCGCCATCTGGCCGAAGCTCTGGGTTATCGGGCAATGGACAGGTTGTTGCAAAAATTGGCGAAAAACTCGGCGTAATGCGCAAAATTTTAAGGCACACCACGCAGTAAGGGCACATTTTCATAAGGTGGGACGGAAGTCg                                                                >  1:531315/1‑251 (MQ=255)                                                                                                                                                                                    gTTCCTGGAAAATGCCCTGACTAAACTGGGGCTTTCCGTACGAGCGTACCACCGCATACTGAAAGTGTCCCGCACCATTGCCGATTTAGATGGAGAACAACACATCAACCAGCGCCATCTGGCCGAAGCTCTGGGTTATCGGGCAATGGACAGGTTGTTGCAAAAATTGGCGAAAAACTCGGCGTAATGCGCAAAATTTTAAGGCACACCACGCAGTAAGGGCACATTTTCATAAGGTGGGACGGAAGTCg                                                                >  1:57664/1‑251 (MQ=255)                                                                                                                                                                                      tCCTGGAAAATGCCCTGACTAAACTGGGGCTTTCCGTACGAGCGTACCACCGCATACTGAAAGTGTCCCGCACCATTGCCGATTTAGATGGAGAACAACACATCAACCAGCGCCATCTGGCCGAAGCTCTGGGTTATCGGGCAATGGACAGGTTGTTGCAAAAATTGGCGAAAAACTCGGCGTAATGCGCAAAATTTTAAGGCACACCACGCAGTAAGGGCACATTTTCATAAGGTGGGACGGAAGTCGGt                                                              <  1:518310/251‑1 (MQ=255)                                                                                                                                                                                           gAAAATGCCCTGACTAAACTGGGGCTTTCCGTACGAGCGTACCACCGCATACTGAAAGTGTCCCGCACCATTGCCGATTTAGATGGAGAACAACACATCAACCAGCGCCATCTGGCCGAAGCTCTGGGTTATCGGGCAATGGACAGGTTGTTGCAAAAATTGGCGAAAAACTCGGCGTAATGCGCAAAATTTTAAGGCACACCACGCAGTAAggg                                                                                             <  1:114069/215‑1 (MQ=255)                                                                                                                                                                                                  cccTGACTAAACTGGGGCTTTCCGTACGAGCGTACCACCGCATACTGAAAGTGTCCCGCACCATTGCCGATTTAGATGGAGAACAACACATCAACCAGCGCCATCTGGCCGAAGCTCTGGGTTATCGGGCAATGGACAGGTTGTTGCAAAAATTGGCGAAAAACTCGGCGTAATGCGCAAAATTTTAAGGCACACCACGCAGTAAGGGCACATTTTCATAAGGTGGGACGGAAGTCGGTGCAGCGGCTTc                                                   <  1:686545/250‑1 (MQ=255)                                                                                                                                                                                                    cTGACTAAACTGGGGCTTTCCGTACGAGCGTACCACCGCATACTGAAAGTGTCCCGCACCATTGCCGATTTAGATGGAGAACAACACATCAACCAGCGCCATCTGGCCGAAGCTCTGGGTTATCGGGCAATGGACAGGTTGTTGCAAAAATTGGCGAAAAACTCGGCGTAATGCGCAAAATTTTAAGGCACACCACGCAGTAAGGGCACATTTTCATAAGGTGGGACGGAAGTCGGTGCAGCGGCTTCACt                                                <  1:610841/251‑1 (MQ=255)                                                                                                                                                                                                      gACTAAACTGGGGCTTTCCGTACGAGCGTACCACCGCATACTGAAAGTGTCCCGCACCATTGCCGATTTAGATGGAGAACAACACATCAACCAGCGCCATCTGGCCGAAGCTCTGGGTTATCGGGCAATGGAc                                                                                                                                                                    >  1:841113/1‑133 (MQ=255)                                                                                                                                                                                                      gACTAAACTGGGGCTTTCCGTACGAGCGTACCACCGCATACTGAAAGTGTCCCGCACCATTGCCGATTTAGATGGAGAACAACACATCAACCAGCGCCATCTGGCCGAAGCTCTGGGTTATCGGGCAATGGACAGGTTGTTGCAAAAATTGGCGAAAAACTCGGCGTAATGCGCAAAATTTTAAGGCACACCACGCAGTAAGGGCACATTTTCATAAGGTGGGACGGAAGTCGGTGCAGCGGCTTCACTaa                                              >  1:127587/1‑251 (MQ=255)                                                                                                                                                                                                                     ttCCGTACGAGCGTACCACCGCATACTGAAAGTGTCCCGCACCATTGCCGATTTAGATGGAGAACAACACATCAACCAGCGCCATCTGGCCGAAGCTCTGGGTTATCGGGCAATGGACAGGTTGTTGCAAAAATTGGCGAAAAACTCGGCGTAATGCGCAAAATTTTAAGGCACACCACGCAGTAAGGGCACATTTTCATAAGGTGGGACGGAAGTCGGTGCAGCGGCTTCACTAAATAAGAAAATAAACg                               >  1:209360/1‑251 (MQ=255)                                                                                                                                                                                                                      tCCGTACGAGCGTACCACCGCATACTGAAAGTGTCCCGCACCATTGCCGATTTAGATGGAGAACAACACATCAACCAGCGCCATCTGGCCGAAGCTCTGGGTTATCGGGCAATGGACAGGTTGTTGCAAAAATTGGCGAAAAACTCGGCGTAATGCGCAAAATTTTAAGGCACACCACGCAGTAAGGGCACATTTTCATAAGGTGGGACGGAAGTCGGTGCAGCGGCTTCACTAAATAAGAAAATAAACgg                              <  1:655406/251‑1 (MQ=255)                                                                                                                                                                                                                       ccGTACGAGCGTACCACCGCATACTGAAAGTGTCCCGCACCATTGCCGATTTAGATGGAGAACAACACATCAACCAGCGCCATCTGGCCGAAGCTCTGGGTTATCGGGCAATGGACAGGTTGTTGCAAAAATTGGCGAAAAACTCGGCGTAATGCGCAAAATTTTAAGGCACACCACGCAGTAAGGGCACATTTTCATAAGGTGGGACGGAAGTCGGTGCAGCGGCTTCACTAAATAAGAAAATAAACGGt                             >  1:34449/1‑251 (MQ=255)                                                                                                                                                                                                                         gTACGAGCGTACCACCGCATACTGAAAGTGTCCCGCACCATTGCCGATTTAGATGGAGAACAACACATCAACCAGCGCCATCTGGCCGAAGCTCTGGGTTATCGGGCAATGGACAGGTTGTTGCAAAAATTGGCGAAAAACTCGGCGTAATGCGCAAAATTTTAAGGCACACCACGCAGTAAGGGCACATTTTCATAAGGTGGGACGGAAGTCGGTGCAGCGGCTTCACTAAATAAGAAAATAAACGGttt                           >  1:734673/1‑251 (MQ=255)                                                                                                                                                                                                                             gAGCGTACCACCGCATACTGAAAGTGTCCCGCACCATTGCCGATTTAGATGGAGAACAACACATCAACCAGCGCCATCTGGCCGAAGCTCTGGGTTATCGGGCAATGGACAGGTTGTTGCAAAAATTGGCGAAAAACTCGGCGTAATGCGCAAAATTTTAAGGCACACCACGCAGTAAGGGCACATTTTCATAAGGTGGGACGGAAGTCGGTGCAGCGGCTTCACTAAATAAGAAAATAAACGGTTTcgg                        >  1:542070/1‑250 (MQ=255)                                                                                                                                                                                                                                      accGCATACTGAAAGTGTCCCGCACCATTGCCGATTTAGATGGAGAACAACACATCAACCAGCGCCATCTGGCCGAAGCTCTGGGTTATCGGGCAATGGACAGGTTGTTGCAAAAATTGGCGAAAAACTCGGCGTAATGCGCAAAATTTTAAGGCACACCACGCAGTAAGGGCACATTTTCATAAGGTGGGACGGAAGTCGGTGCAGCGGCTTCACTAAATAAGAAAATAAACGGTTTCGGCGGAATAAc               >  1:664265/1‑250 (MQ=255)                                                                                                                                                                                                                                           aTACTGAAAGTGTCCCGCACCATTGCCGATTTAGATGGAGAACAACACATCAACCAGCGCCATCTGGCCGAAGCTCTGGGTTATCGGGCAATGGACAGGTTGTTGCAAAAATTGGCGAAAAACTCGGCGTAATGCGCAAAATTTTAAGGCACACCACGCAGTAAGGGCACATTTTCATAAGGTGGGACGGAAGTCGGTGCAGCGGCTTCACTAAATAAGAAAATAAACGGTTTCGGCGGAATAACCtttt          >  1:650717/1‑250 (MQ=255)                                                                                                                                                                                                                                            tACTGAAAGTGTCCCGCACCATTGCCGATTTAGATGGAGAACAACACATCAACCAGCGCCATCTGGCCGAAGCTCTGGGTTATCGGGCAATGGACAGGTTGTTGCAAAAATTGGCGAAAAACTCGGCGTAATGCGCAAAATTTTAAGGCACACCACGCAGTAAGGGCACATTTTCATAAGGTGGGACGGAAGTCGGTGCAGCGGCTTCACTAAATAAGaa                                       >  1:232611/1‑220 (MQ=255)                                                                                                                                                                                                                                              cTGAAAGTGTCCCGCACCATTGCCGATTTAGATGGAGAACAACACATCAACCAGCGCCATCTGGCCGAAGCTCTGGGTTATCGGGCAATGGACAGGTTGTTGCAAAAATTGGCGAAAAACTCGGCGTAATGCGCAAAATTTTAAGGCACACCACGCAGTAAggg                                                                                             >  1:324727/1‑164 (MQ=255)                                                                                                                                                                                                                                                  aaGTGTCCCGCACCATTGCCGATTTAGATGGAGAACAACACATCAACCAGCGCCATCTGGCCGAAGCTCTGGGTTATCGGGCAATGGACAGGTTGTTGCAAAAATTGGCGAAAAACTCGGCGTAATGCGCAAAATTTTAAGGCACACCACGCAGTAAGGGCACATTTTCATAAGGTGGGACGGAAGTCGGTGCAGCGGCTTCACTAAATAAGAAAATAAACGGTTTCGGCGGAATAACCTTTTCATTACg   >  1:727152/1‑250 (MQ=255)                                                                                                                                                                                                                                                  aaGTGTCCCGCACCATTGCCGATTTAGATGGAGAACAACACATCAACCAGCGCCATCTGGCCGAAGCTCTGGGTTATCGGGCAATGGACAGGTTGTTGCAAAAATTGGCGAAAAACTCGGCGTAATGCGCAAAATTTTAAGGCACACCACGCAGTAAGGGCACATTTTCATAAGGTGGGACGGAAGTCGGTGCAGCGGCTTCACTAAATAAGAAAATAAACGGTTTCGGCGGAATAACCTTTTCATTACGc  <  1:623542/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      CCCTTATTACCACAAGGCAGCTTACAAAATAGCGGCGATCGTGGCGAATCCAGCGCAACGGTGCGAGAAAAAGTCCTGAAAACCCGTGCCATTCAACTTCAGCGCGCCGGCAAAATTAATGCCCATTTAAATAGCAAAGAAATCGAACGGGACTGCAAACTAACCGACAAAGACGCACAGTTCCTGGAAAATGCCCTGACTAAACTGGGGCTTTCCGTACGAGCGTACCACCGCATACTGAAAGTGCCCCGCACCATTGCCGATTTAGATGGAGAACAACACATCAACCAGCGCCATCTGGCCGAAGCTCTGGGTTATCGGGCAATGGACAGGTTGTTGCAAAAATTGGCGAAAAACTCGGCGTAATGCGCAAAATTTTAAGGCACACCACGCAGTAAGGGCACATTTTCATAAGGTGGGACGGAAGTCGGTGCAGCGGCTTCACTAAATAAGAAAATAAACGGTTTCGGCGGAATAACCTTTTCATTACGC  >  1/167387‑167878

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑