Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,278,687	T→C	intergenic (+139557/+114624)	unknown → / ← unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,278,687	0	T	C	100.0%	126.2 / NA	45	intergenic (+139557/+114624)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/21);  total (24/21)

AAATAATGCATTGTGAGATCCCTTAATGCTTGAAACATCACAAACAATCCCCGAACTGGTTTCATGGGCGAAAGAACGCGAATTTTCGCTGAATTTACCCACCGATCGTCTGGCGTTTTTATTGGCAATTGCCATTTATAATAACGAACGTTTTGACGGTGAAATGATCGAATCGGATCTGGTGGACATTTTTCGCCACGTTTCCGGCGCCTTTGACCAATCCCAGGAAACCATTGCTACCCGTGCCAATAACGCCATTAACGAATTGGTAAAACAACGCTTTCTTAACCGCTTCAGCAGTGAATTTACCGAAGGTTTGGCGATTTATCGCTTAACCCCGCTGGGTGTCGGCGTCTCCGATTACTACATTCGTCAACGCGAATTTTCCGCCTTGCGCCTTTCCGTTCAGCTTTCCATTGTCGCCGATGAAATTCAACGTGCCTCTGACGCCGCCGAAGAAGATGGCGACGAACATTATTGG  >  1/1278444‑1278924                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                              aaaTAATGCATTGTGAGATCCCTTAATGCTTGAAACATCACAAACAATCCCCGAACTGGTTTCATGGGCGAAAGAACGCGAATTTTCGCTGAATTTACCCACCGATCGTCTGGCGTTTTTATTGGCAATTGCCATTTATAATAACGAACGTTTTGACGGTGAAATGATCGAATCGGATCTGGTGGACATTTTTCGCCACGTTTCCGGCGCCTTTGACCAATCCCAGGAAACCATTGCTACCCGCGCCAATa                                                                                                                                                                                                                                        >  1:27990/1‑251 (MQ=255)   ataatGCATTGTGAGATCCCTTAATGCTTGAAACATCACAAACAATCCCCGAACTGGTTTCATGGGCGAAAGAACGCGAATTTTCGCTGAATTTACCCACCGATCGTCTGGCGTTTTTATTGGCAATTGCCATTTATAATAACGAACGTTTTGACGGTGAAATGATCGACTCGGATCTGGTGGACATTTTTCGCCACGTTTCCGGCGCCTTTGACCAATCCCAGGAAACCATTGCTACCCGCGCCAATaa                                                                                                                                                                                                                                       >  1:445976/1‑250 (MQ=255)   ataatGCATTGTGAGATCCCTTAATGCTTGAAACATCACAAACAATCCCCGAACTGGTTTCATGGGCGAAAGAACGCGAATTTTCGCTGAATTTACCCACCGATCGTCTGGCGTTTTTATTGGCAATTGCCATTTATAATAACGAACGTTTTGACGGTGAAATGATCGAATCGGATCTGGTGGACATTTTTCGCCACGTTTCCGGCGCCTTTGACCAATCCCAGGAAACCATTGCTACCCGCGCCAATaa                                                                                                                                                                                                                                       >  1:305819/1‑250 (MQ=255)       tGCATTGTGAGATCCCTTAATGCTTGAAACATCACAAACAATCCCCGAACTGGTTTCATGGGCGAAAGAACGCGAATTTTCGCTGAATTTACCCACCGATCGTCTGGCGTTTTTATTGGCAATTGCCATTTATAATAACGAACGTTTTGACGGTGAAATGATCGAATCGGATCTGGTGGACATTTTTCGCCACGTTTCCGGCGCCTTTGACCAATCCCAGGAAACCATTGCTACCCGCGCCAATAACGCCa                                                                                                                                                                                                                                  >  1:900462/1‑251 (MQ=255)              tgAGATCCCTTAATGCTTGAAACATCACAAACAATCCCCGAACTGGTTTCATGGGCGAAAGAACGCGAATTTTCGCTGAATTTACCCACCGATCGTCTGGCGTTTTTATTGGCAATTGCCATTTATAATAACGAACGTTTTGACGGTGAAATGATCGAATCGGATCTGGTGGACATTTTTCGCCACGTTTCCGGCGCCTTTGACCAATCCCAGGAAACCATTGCTACCCGCGCCAATAACGCCATTAACg                                                                                                                                                                                                                            <  1:64133/250‑1 (MQ=255)                    cccTTAATGCTTGAAACATCACAAACAATCCCCGAACTGGTTTCATGGGCGAAAGAACGCGAATTTTCGCTGAATTTACCCACCGATCGTCTGGCGTTTTTATTGGCAATTGCCATTTATAATAACGAACGTTTTGACGGTGAAATGATCGAATCGGATCTGGTGGACATTTTTCGCCACGTTTCCGGCGCCTTTGACCAATCCCAGGAAACCATTGCTACCCGCGCCAATAACGCCATTAACGAATTGGt                                                                                                                                                                                                                     >  1:776953/1‑251 (MQ=255)                     ccTTAATGCTTGAAACATCACAAACAATCCCCGAACTGGTTTCATGGGCGAAAGAACGCGAATTTTCGCTGAATTTACCCACCGATCGTCTGGCGTTTTTATTGGCAATTGCCATTTATAATAACGAACGTTTTGACGGTGAAATGATCGAATCGGATCTGGTGGACATTTTTCGCCACGTTTCCGGCGCCTTTGACCAATCCCAGGAAACCATTGCTACCCGCGCCAATAACGCCATTAACGAATTGGTa                                                                                                                                                                                                                    >  1:155048/1‑251 (MQ=255)                                     aTCACAAACAATCCCCGAACTGGTTTCATGGGCGAAAGAACGCGAATTTTCGCTGAATTTACCCACCGATCGTCTGGCGTTTTTATTGGCAATTGCCATTTATAATAACGAACGTTTTGACGGTGAAATGATCGAATCGGATCTGGTGGACATTTTTCGCCACGTTTCCGGCGCCTTTGACCAATCCCAGGAAACCATTGCTACCCGCGCCAATaa                                                                                                                                                                                                                                       >  1:184322/1‑216 (MQ=255)                                     aTCACAAACAATCCCCGAACTGGTTTCATGGGCGAAAGAACGCGAATTTTCGCTGAATTTACCCACCGATCGTCTGGCGTTTTTATTGGCAATTGCCATTTATAATAACGAACGTTTTGACGGTGAAATGATCGAATCGGATCTGGTGGACATTTTTCGCCACGTTTCCGGCGCCTTTGACCAATCCCAGGAAACCATTGCTACCCGCGCCAATAACGCCATTAACGAATTGGTAAAACAACGCTTTCTTa                                                                                                                                                                                                    >  1:530505/1‑251 (MQ=255)                                        acaaacaaTCCCCGAACTGGTTTCATGGGCGAAAGAACGCGAATTTTCGCTGAATTTACCCACCGATCGTCTGGCGTTTTTATTGGCAATTGCCATTTATAATAACGAACGTTTTGACGGTGAAATGATCGAATCGGATCTGGTGGACATTTTTCGCCACGTTTCCGGCGCCTTTGACCAATCCCAGGAAACCATTGCTACCCGCGCCAATAACGCCATTAACGAATTGGTAAAACAACGCTTTCTTAAcc                                                                                                                                                                                                 <  1:667387/251‑1 (MQ=255)                                                tCCCCGAACTGGTTTCATGGGCGAAAGAACGCGAATTTTCGCTGAATTTACCCACCGATCGTCTGGCGTTTTTATTGGCAATTGCCATTTATAATAACGAACGTTTTGACGGTGAAATGATCGAATCGGATCTGGTGGACATTTTTCGCCACGTTTCCGGCGCCTTTGACCAATCCCAGGAAACCATTGCTACCCGCGCCAATAACGCCATTAACGAATTGGTAAAACAACGCTTTCTTAACCGCTTcag                                                                                                                                                                                          >  1:418707/1‑250 (MQ=255)                                                       aCTGGTTTCATGGGCGAAAGAACGCGAATTTTCGCTGAATTTACCCACCGATCGTCTGGCGTTTTTATTGGCAATTGCCATTTATAATAACGAACGTTTTGACGGTGAAATGATCGAATCGGATCTGGTGGACATTTTTCGCCACGTTTCCGGCGCCTTTGACCAATCCCAGGAAACCATTGCTACCCGCGCCAATAACGCCATTAACGAATTGGTAAAACAACGCTTTCTTAACCGCTTCAGCAGTGAAt                                                                                                                                                                                  <  1:830787/251‑1 (MQ=255)                                                          ggTTTCATGGGCGAAAGAACGCGAATTTTCGCTGAATTTACCCACCGATCGTCTGGCGTTTTTATTGGCAATTGCCATTTATAATAACGAACGTTTTGACGGTGAAATGATCGAATCGGATCTGGTGGACATTTTTCGCCACGTTTCCGGCGCCTTTGACCAATCCCAGGAAACCATTGCTACCCGCGCCAATaa                                                                                                                                                                                                                                       <  1:496256/195‑1 (MQ=255)                                                          ggTTTCATGGGCGAAAGAACGCGAATTTTCGCTGAATTTACCCACCGATCGTCTGGCGTTTTTATTGGCAATTGCCATTTATAATAACGAACGTTTTGACGGTGAAATGATCGAATCGGATCTGGTGGACATTTTTCGCCACGTTTCCGGCGCCTTTGACCAATCCCAGGAAACCATTGCTACCCGCGCCAATAACGCCATTAACGAAt                                                                                                                                                                                                                         <  1:261817/209‑1 (MQ=255)                                                            tttCATGGGCGAAAGAACGCGAATTTTCGCTGAATTTACCCACCGATCGTCTGGCGTTTTTATTGGCAATTGCCATTTATAATAACGAACGTTTTGACGGTGAAATGATCGAATCGGATCTGGTGGACATTTTTCGCCACGTTTCCGGCGCCTTTGACCAATCCCAGGAAACCATTGCTACCCGCGCCAATAACGCCATTAACGAATTGGTAAAACAACGCTTTCTTAACCGCTTCAGCAGTGAATTTAc                                                                                                                                                                              <  1:694225/250‑1 (MQ=255)                                                                 tGGGCGAAAGAACGCGAATTTTCGCTGAATTTACCCACCGATCGTCTGGCGTTTTTATTGGCAATTGCCATTTATAATAACGAACGTTTTGACGGTGAAATGATCGAATCGGATCTGGTGGACATTTTTCGCCACGTTTCCGGCGCCTTTGACCAATCCCAGGAAACCATTGCTACCCGCGCCAATAACGCCATTAACGAATTGGTAAAACAACGCTTTCTTAACCGCTTCAGCAGTGAATTTACCGAAgg                                                                                                                                                                        <  1:8803/251‑1 (MQ=255)                                                                       aaaGAACGCGAATTTTCGCTGAATTTACCCACCGATCGTCTGGCGTTTTTATTGGCAATTGCCATTTATAATAACGAACGTTTTGACGGTGAAATGATCGAATCGGATCTGGTGGACATTTTTCGCCACGTTTCCGGCGCCTTTGACCAATCCCAGGAAACCATTGCTACCCGCGCCAATAACGCCATTAACGAATTGGTAAAACAACGCTTTCTTAAc                                                                                                                                                                                                  >  1:631983/1‑219 (MQ=255)                                                                                                tACCCACCGATCGTCTGGCGTTTTTATTGGCAATTGCCATTTATAATAACGAACGTTTTGACGGTGAAATGATCGAATCGGATCTGGTGGACATTTTTCGCCACGTTTCCGGCGCCTTTGACCAATCCCAGGAAACCATTGCTACCCGCGCCAATAACGCCATTAACGAATTGGTAAAACAACGCTTTCTTAACCGCTTCAGCAGTGAATTTACCGAAGGTTTGGCGATTTATCGCTTAACCCCGCTGGgt                                                                                                                                         <  1:778271/251‑1 (MQ=255)                                                                                                      ccGATCGTCTGGCGTTTTTATTGGCAATTGCCATTTATAATAACGAACGTTTTGACGGTGAAATGATCGAATCGGATCTGGTGGACATTTTTCGCCACGTTTCCGGCGCCTTTGACCAATCCCAGGAAACCATTGCTACCCGCGCCAATAACGCCATTAACGAATTGGTAAAACAACGCTTTCTTAACCGCTTCAGCAGTGAATTTACCGAAGGTTTGGCGATTTATCGCTTAACCCCGCTGGGTGTCgg                                                                                                                                    >  1:61886/1‑250 (MQ=255)                                                                                                       cGATCGTCTGGCGTTTTTATTGGCAATTGCCATTTATAATAACGAACGTTTTGACGGTGAAATGATCGAATCGGATCTGGTGGACATTTTTCGCCACGTTTCCGGCGCCTTTGACCAATCCCAGGAAACCATTGCTACCCGCGCCAATAACGCCATTAACGAATTGGTAAAACAACGCTTTCTTAACCGCTTCAGCAGTGAATTTACCGAAGGTTTGGCGATTTATCGCTTAACCCCGCTGGGTGTCGGCg                                                                                                                                  <  1:122684/251‑1 (MQ=255)                                                                                                         aTCGTCTGGCGTTTTTATTGGCAATTGCCATTTATAATAACGAACGTTTTGACGGTGAAATGATCGAATCGGATCTGGTGGACATTTTTCGCCACGTTTCCGGCGCCTTTGACCAATCCCAGGAAACCATTGCTACCCGCGCCAATAACGCCATTAACGAATTGGTAAAACAACGCTTTCTTAACCGCTTCAGCAGTGAATTTACCGAAGGTTTGGCGATTTATCGCTTAACCCCGCTGGGTGTCGGCGt                                                                                                                                 >  1:753349/1‑250 (MQ=255)                                                                                                                 gCGTTTTTATTGGCAATTGCCATTTATAATAACGAACGTTTTGACGGTGAAATGATCGAATCGGATCTGGTGGACATTTTTCGCCACGTTTCCGGCGCCTTTGACCAATCCCAGGAAACCATTGCTACCCGCGCCAATAACGCCATTAACGAATTGGTAAAACAACGCTTTCt                                                                                                                                                                                                      >  1:589529/1‑173 (MQ=255)                                                                                                                     ttttATTGGCAATTGCCATTTATAATAACGAACGTTTTGACGGTGAAATGATCGAATCGGATCTGGTGGACATTTTTCGCCACGTTTCCGGCGCCTTTGACCAATCCCAGGAAACCATTGCTACCCGCGCCAATAACGCCATTAACGAATTGGTAAAACAACGCTTTCTTAACCGCTTCAGCAGTGAATTTACCGAAGGTTTGGCGATTTATCGCTTAACCCCGCTGGGTGTCGGCGTCTCCGATtactac                                                                                                                    <  1:502716/251‑1 (MQ=255)                                                                                                                       ttATTGGCAATTGCCATTTATAATAACGAACGTTTTGACGGTGAAATGATCGAATCGGATCTGGTGGACATTTTTCGCCACGTTTCCGGCGCCTTTGACCAATCCCAGGAAACCATTGCTACCCGCGCCAATAACGCCATTAACGAATTGGTAAAACAACGCTTTCTTAACCGCTTCAGCAGTGAATTTACCGAAGGTTTGGCGATTTATCGCTTAACCCCGCTGGGTGTCGGCGTCTCCGATTACTACAt                                                                                                                  <  1:106752/251‑1 (MQ=255)                                                                                                                       ttATTGGCAATTGCCATTTATAATAACGAACGTTTTGACGGTGAAATGATCGAATCGGATCTGGTGGACATTTTTCGCCACGTTTCCGGCGCCTTTGACCAATCCCAGGAAACCATTGCTACCCGCGCCAATAACGCCATTAACGAATTGGTAAAACAACGCTTTCTTAACCGCTTCAGCAGTGAATTTACCGAAGGTTTGGCGATTTATCGCTTAACCCCGCTGGGTGTCGGCGTCTCCGATTACTACAt                                                                                                                  <  1:838877/251‑1 (MQ=255)                                                                                                                            ggCAATTGCCATTTATAATAACGAACGTTTTGACGGTGAAATGATCGAATCGGATCTGGTGGACATTTTTCGCCACGTTTCCGGCGCCTTTGACCAATCCCAGGAAACCATTGCTACCCGCGCCAATAACGCCATTAACGAATTGGTAAAACAACGCTTTCTTAACCGCTTCAGCAGTGAATTTACCGAAGGTTTGGCGATTTATCGCTTAACCCCGCTGGGTGTCGGCGTCTCCGATTACTACATTCGt                                                                                                              >  1:824955/1‑250 (MQ=255)                                                                                                                                        ttATAATAACGAACGTTTTGACGGTGAAATGATCGAATCGGATCTGGTGGACATTTTTCGCCACGTTTCCGGCGCCTTTGACCAATCCCAGGAAACCATTGCTACCCGCGCCAATAACGCCATTAACGAATTGGTAAAACAACGCTTTCTTAACCGCTTCAGCAGTGAATTTACCGAAGGTTTGGCGATTTATCGCTTAACCCCGCTGGGTGTCGGCGTCTCCGATTACTACATTCGTCAACGCGAAtttt                                                                                                 >  1:881063/1‑251 (MQ=255)                                                                                                                                              taaCGAACGTTTTGACGGTGAAATGATCGAATCGGATCTGGTGGACATTTTTCGCCACGTTTCCGGCGCCTTTGACCAATCCCAGGAAACCATTGCTACCCGCGCCAATAACGCCATTAACGAATTGGTAAAACAACGCTTTCTTAACCGCTTCAGCAGTGAATTTACCg                                                                                                                                                                            >  1:50943/1‑170 (MQ=255)                                                                                                                                                     cgTTTTGACGGTGAAATGATCGAATCGGATCTGGTGGACATTTTTCGCCACGTTTCCGGCGCCTTTGACCAATCCCAGGAAACCATTGCTACCCGCGCCAATAACGCCATTAACGAATTGGTAAAACAACGCTTTCTTAACCGCTTCAGCAGTGAATTTACCGAAGGTTTGGCGATTTATCGCTTAACCCCGCTGGGTGTCGGCGTCTCCGATTACTACATTCGTCAACGCGAATTTTCCGCCTTgcgc                                                                                      >  1:403944/1‑249 (MQ=255)                                                                                                                                                     cgTTTTGACGGTGAAATGATCGAATCGGATCTGGTGGACATTTTTCGCCACGTTTCCGGCGCCTTTGACCAATCCCAGGAAACCATTGCTACCCGCGCCAATAACGCCATTAACGAATTGGTAAAACAACGCTTTCTTAACCGCTTCAGCAGTGAATTTACCGAAGGTTTGGCGATTTATCGCTTAACCCCGCTGGGTGTCGGCGTCTCCGATTACTACATTCGTCAACGCGAATTTTCCGCCTTGCGcc                                                                                     <  1:448196/250‑1 (MQ=255)                                                                                                                                                        tttGACGGTGAAATGATCGAATCGGATCTGGTGGACATTTTTCGCCACGTTTCCGGCGCCTTTGACCAATCCCAGGAAACCATTGCTACCCGCGCCAATAACGCCATTAACGAATTGGTAAAACAACGCTTTCTTAACCGCTTCAGCAGTGAATTTACCGAAGGTTTGGCGATTTATCGCTTAACCCCGCTGGGTGTCGGCGTCTCCGATTACTACATTCGTCAACGCGAATTTTCCGCCTTGCGCCTTTc                                                                                 <  1:343765/251‑1 (MQ=255)                                                                                                                                                             cGGTGAAATGATCGAATCGGATCTGGTGGACATTTTTCGCCACGTTTCCGGCGCCTTTGACCAATCCCAGGAAACCATTGCCACCCGCGCCAATAACGCCATTAACGAATTGGTAAAACAACGCTTTCTTAACCGCTTCAGCAGTGAATTTACCGAAGGTTTGGCGATTTATCGCTTAACCCCGCTGGGTGTCGGCGTCTCCGATTACTACATTCGTCAACGCGAATTTTCCGCCTTGCGCCTTTCCGTTc                                                                            <  1:424008/251‑1 (MQ=255)                                                                                                                                                               gTGAAATGATCGAATCGGATCTGGTGGACATTTTTCGCCACGTTTCCGGCGCCTTTGACCAATCCCAGGAAACCATTGCTACCCGCGCCAATAACGCCATTAACGAATTGGTAAAACAACGCTTTCTTAACCGCTTCAGCAGTGAATTTACCGAAGGTTTGGCGATTTATCGCTTAACCCCGCTGGGTGTCGGCGTCTCCGATTACTACATTCGTCAACGCGAATTTTCCGCCTTGCGCCTTTCCGTTCAg                                                                          >  1:38151/1‑251 (MQ=255)                                                                                                                                                                       aTCGAATCGGATCTGGTGGACATTTTTCGCCACGTTTCCGGCGCCTTTGACCAATCCCAGGAAACCATTGCTACCCGCGCCAATAACGCCATTAACGAATTGGTAAAACAACGCTTTCTTAACCGCTTCAGCAGTGAATTTACCGAAGGTTTGGCGATTTATCGCTTAACCCCGCTGGGTGTCGGCGTCTCCGATTACTACATTCGTCAACGCGAATTTTCCGCCTTGCGCCTTTCCGTTCAGCTTTCCAt                                                                  <  1:453545/251‑1 (MQ=255)                                                                                                                                                                           aaTCGGATCTGGTGGACATTTTTCGCCACGTTTCCGGCGCCTTTGACCAATCCCAGGAAACCATTGCTACCCGCGCCAATAACGCCATTAACGAATTGGTAAAACAACGCTTTCTTAACCGCTTCAGCAGTGAATTTACCGAAGGTTTGGCGATTTATCGCTTAACCCCGCTGGGTGTCGGCGTCTCCGATTACTACATTCGTCAACGCGAATTTTCCGCCTTGCGCCTTTCCGTTCAGCTTTCCATTGt                                                               <  1:780617/250‑1 (MQ=255)                                                                                                                                                                                             tttttCGCCACGTTTCCGGCGCCTTTGACCAATCCCAGGAAACCATTGCTACCCGCGCCAATAACGCCATTAACGAATTGGTAAAACAACGCTTTCTTAACCGCTTCAGCAGTGAATTTACCGAAGGTTTGGCGATTTATCGCTTAACCCCGCTGGGTGTCGGCGTCTCCGATTACTACATTCGTCAACGCGAATTTTCCGCCTTGCGCCTTTCCGTTCAGCTTTCCATTGTCGCCGATGAAATTCAACGt                                            >  1:599057/1‑251 (MQ=255)                                                                                                                                                                                                      aCGTTTCCGGCGCCTTTGACCAATCCCAGGAAACCATTGCTACCCGCGCCAATAACGCCATTAACGAATTGGTAAAACAACGCTTTCTTAACCGCTTCAGCAGTGAATTTACCGAAGGTTTGGCGATTTATCGCTTAACCCCGCTGGGTGTCGGCGTCTCCGATTACTACATTCGTCAACGCGAATTTTCCGCCTTGCGCCTTTCCGTTCAGCTTTCCATTGt                                                               <  1:642325/223‑1 (MQ=255)                                                                                                                                                                                                       cGTTTCCGGCGCCTTTGACCAATCCCAGGAAACCATTGCTACCCGCGCCAATAACGCCATTAACGAATTGGTAAAACAACGCTTTCTTAACCGCTTCAGCAGTGAATTTACCGAAGGTTTGGCGATTTATCGCTTAACCCCGCTGGGTGTCGGCGTCTCCGATTACTACATTCGTCAACGCGAATTTTCCGCCTTGCGCCTTTCCGTTCAGCTTTCCATTGTCGCCggcggcgttaagggtatcttgtagt                                  >  1:62723/1‑227 (MQ=255)                                                                                                                                                                                                          ttCCGGCGCCTTTGACCAATCCCAGGAAACCATTGCTACCCGCGCCAATAACGCCATTAACGAATTGGTAAAACAACGCTTTCTTAAcc                                                                                                                                                                                                 <  1:106451/89‑1 (MQ=255)                                                                                                                                                                                                                  ccTTTGACCAATCCCAGGAAACCATTGCTACCCGCGCCAATAACGCCATTAACGAATTGGTAAAACAACGCTTTCTTAACCGCTTCAGCAGTGAATTTACCGAAGGTTTGGCGATTTATCGCTTAACCCCGCTGGGTGTCGGCg                                                                                                                                  >  1:152104/1‑144 (MQ=255)                                                                                                                                                                                                                       gACCAATCCCAGGAAACCATTGCTACCCGCGCCAATAACGCCATTCACGAATTGGTAAAACAACGCTTTCTTAACCGCTTCAGCAGTGAATTTACCGAAGGTTTGGCGATTTATCGCTTAACCCCGCTGGGTGTCGGCGTCTCCGATTACTACATTCGTCAACGCGAATTTTCCGCCTTGCGCCTTTCCGTTCAGCTTTCCATTGTCGCCGATGAAATTCAACGTGCCTCTGACGCCGCCGAAGAAGATgg                  >  1:414211/1‑251 (MQ=255)                                                                                                                                                                                                                        aCCAATCCCAGGAAACCATTGCTACCCGCGCCAATAACGCCATTAACGAATTGGTAAAACAACGCTTTCTTAACCGCTTCAGCAGTGAATTTACCGAAGGTTTGGCGATTTATCGCTTAACCCCGCTGGGTGTCGGCGTCTCCGATTACTACATTCGTCAACGCGAATTTTCCGCCTTGCGCCTTTCCGTTCAGCTTTCCATTGTCGCCGATGAAATTCAACGTGCCTCTGACGCCGCCGAAGAAGATGGc                 <  1:691256/251‑1 (MQ=255)                                                                                                                                                                                                                            aTCCCAGGAAACCATTGCTACCCGCGCCAATAACGCCATTAACGAATTGGTAAAACAACGCTTTCTTAACCGCTTCAGCAGTGAATTTACCGAAGGTTTGGCGATTTATCGCTTAACCCCGCTGGGTGTCGGCGTCTCCGATTACTACATTCGTCAACGCGAATTTTCCGCCTTGCGCCTTTCCGTTCAGCTTTCCATTGTCGCCGATGAAATTCAACGTGCCTCTGACGCCGCCGAAGAAGATGGcgacg             >  1:421035/1‑251 (MQ=255)                                                                                                                                                                                                                             tCCCAGGAAACCATTGCTACCCGCGCCAATAACGCCATTAACGAATTGGTAAAACAACGCTTTCTTAACCGCTTCAGCAGTGAATTTACCGAAGGTTTGGCGATTTATCGCTTAACCCCGCTGGGTGTCGGCGTCTCCGATTACTACATTCGTCAACGCGAATTTTCCGCCTTGCGCCTTTCCGTTCAGCTTTCCATTGTCGCCGATGAAATTCAACGTGCCTCTGACGCCGCCGAAGAAGAt                    >  1:141034/1‑243 (MQ=255)                                                                                                                                                                                                                                       ccATTGCTACCCGCGCCAATAACGCCATTAACGAATTGGTAAAACAACGCTTTCTTAACCGCTTCAGCAGTGAATTTACCGAAGGTTTGGCGATTTATCGCTTAACCCCGCTGGGTGTCGGCGTCTCCGATTACTACATTCGTCAACGCGAATTTTCCGCCTTGCGCCTTTCCGTTCAGCTTTCCATTGTCGCCGATGAAATTCAACGTGCCTCTGACGCCGCCGAAGAAGATGGCGACGAACATTATTgg  <  1:770533/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                              AAATAATGCATTGTGAGATCCCTTAATGCTTGAAACATCACAAACAATCCCCGAACTGGTTTCATGGGCGAAAGAACGCGAATTTTCGCTGAATTTACCCACCGATCGTCTGGCGTTTTTATTGGCAATTGCCATTTATAATAACGAACGTTTTGACGGTGAAATGATCGAATCGGATCTGGTGGACATTTTTCGCCACGTTTCCGGCGCCTTTGACCAATCCCAGGAAACCATTGCTACCCGTGCCAATAACGCCATTAACGAATTGGTAAAACAACGCTTTCTTAACCGCTTCAGCAGTGAATTTACCGAAGGTTTGGCGATTTATCGCTTAACCCCGCTGGGTGTCGGCGTCTCCGATTACTACATTCGTCAACGCGAATTTTCCGCCTTGCGCCTTTCCGTTCAGCTTTCCATTGTCGCCGATGAAATTCAACGTGCCTCTGACGCCGCCGAAGAAGATGGCGACGAACATTATTGG  >  1/1278444‑1278924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑