Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,270,629	C→G	intergenic (+131499/+122682)	unknown → / ← unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,270,629	0	C	G	100.0%	214.2 / NA	70	intergenic (+131499/+122682)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (31/39);  total (31/39)

GATTATCTCTATGAAATGCTGAATCGGGAAGTCAACACGCCTTGCACCTCTCACGACTTTGGTAAAGACGTATTGCCGAAATGTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTCGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAATTAACGAAGGCTCGCAAATCGATCATTCCGTGGTGTTACCGCAAGTGGAAATCGGGAAAAACTGCGTGTTAAC  >  1/1270399‑1270874                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      gATTATCTCTATGAAATGCTGAATCGGGAAGTCAACACGCCTTGCACCTCTCACGACTTTGGTAAAGACGTATTGCCGAAATGTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAAc                                                                                                                                                                                                                                      >  1:278343/1‑248 (MQ=255)              aaaTGCTGAATCGGGAAGTCAACACGCCTTGCACCTCTCACGACTTTGGTAAAGACGTATTGCCGAAATGTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGt                                                                                                                                                                                                                      <  1:774461/251‑1 (MQ=255)                     gAATCGGGAAGTCAACACGCCTTGCACCTCTCACGACTTTGGTAAAGACGTATTGCCGAAATGTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCgg                                                                                                                                                                                                               >  1:593244/1‑251 (MQ=255)                       aTCGGGAAGTCAACACGCCTTGCACCTCTCACGACTTTGGTAAAGACGTATTGCCGAAATGTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTg                                                                                                                                                                                                             >  1:34421/1‑251 (MQ=255)                           ggAAGTCAACACGCCTTGCACCTCTCACGACTTTGGTAAAGACGTATTGCCGAAATGTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAg                                                                                                                                                                                                         >  1:761009/1‑251 (MQ=255)                                     acGCCTTGCACCTCTCACGACTTTGGTAAAGACGTATTGCCGAAATGTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATc                                                                                                                                                                                               <  1:373769/251‑1 (MQ=255)                                       gCCTTGCACCTCTCACGACTTTGGTAAAGACGTATTGCCGAAATGTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATcc                                                                                                                                                                                                  <  1:417352/246‑1 (MQ=255)                                          ttGCACCTCTCACGACTTTGGTAAAGACGTATTGCCGAAATGTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAAt                                                                                                                                                                                          <  1:568340/251‑1 (MQ=255)                                                 tctcACGACTTTGGTAAAGACGTATTGCCGAAATGTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATcc                                                                                                                                                                                                                 <  1:881705/221‑1 (MQ=255)                                                 tctcACGACTTTGGTAAAGACGTATTGCCGAAATGTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATcc                                                                                                                                                                                                  <  1:820426/236‑1 (MQ=255)                                                 tctcACGACTTTGGTAAAGACGTATTGCCGAAATGTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTta                                                                                                                                                                                   >  1:271088/1‑251 (MQ=255)                                                 tctcACGACTTTGGTAAAGACGTATTGCCGAAATGTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTta                                                                                                                                                                                   >  1:269452/1‑251 (MQ=255)                                                  ctcACGACTTTGGTAAAGACGTATTGCCGAAATGTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTtat                                                                                                                                                                                  >  1:691597/1‑251 (MQ=255)                                                        aCTTTGGTAAAGACGTATTGCCGAAATGTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATaaaaa                                                                                                                                                                             >  1:862850/1‑250 (MQ=255)                                                             ggTAAAGACGTATTGCCGAAATGTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCaa                                                                                                                                                                       >  1:438632/1‑251 (MQ=255)                                                                aaaGACGTATTGCCGAAATGTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAatat                                                                                                                                                                    <  1:455437/251‑1 (MQ=255)                                                                     cGTATTGCCGAAATGTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAAc                                                                                                                                                               <  1:844517/251‑1 (MQ=255)                                                                     cGTATTGCCGAAATGTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAAc                                                                                                                                                               <  1:700332/251‑1 (MQ=255)                                                                     cGTATTGCCGAAATGTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATAGCAAAc                                                                                                                                                               <  1:268593/251‑1 (MQ=255)                                                                        aTTGCCGAAATGTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCgg                                                                                                                                                            <  1:747721/251‑1 (MQ=255)                                                                             gcatttcatagagataatcggcgTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGcc                                                                                                                                                                                                       <  1:801907/183‑1 (MQ=255)                                                                                   gTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCttt                                                                                                                                                                                     <  1:872258/215‑1 (MQ=255)                                                                                   gTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAAct                                                                                                                                                   >  1:84025/1‑249 (MQ=255)                                                                                            aagGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCgg                                                                                                                                         <  1:877786/250‑1 (MQ=255)                                                                                                   gTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCttt                                                                                                                                                                                     <  1:33752/199‑1 (MQ=255)                                                                                                    ttATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAAc                                                                                                                                                               >  1:844309/1‑220 (MQ=255)                                                                                                    ttATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTa                                                                                                                                             <  1:767300/238‑1 (MQ=255)                                                                                                      ataCGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCg                                                                                                                              <  1:271412/251‑1 (MQ=255)                                                                                                        aCGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTa                                                                                                                                                                                                                                               <  1:181631/136‑1 (MQ=255)                                                                                                               cccGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCg                                                                                                                     >  1:745837/1‑251 (MQ=255)                                                                                                               cccGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCg                                                                                                                     >  1:446270/1‑251 (MQ=255)                                                                                                                ccGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCgg                                                                                                                                         <  1:9265/230‑1 (MQ=255)                                                                                                                           cTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGatat                                                                                                                                                                                                                                                  <  1:434690/114‑1 (MQ=255)                                                                                                                           cTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCatcaatc                                                                                                          <  1:556012/250‑1 (MQ=255)                                                                                                                           cTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCatcaa                                                                                                            <  1:68321/248‑1 (MQ=255)                                                                                                                                gCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGt                                                                                                                                        >  1:106719/1‑215 (MQ=255)                                                                                                                                gCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTAt                                                                                                    >  1:469734/1‑251 (MQ=255)                                                                                                                                  aTGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTc                                                                                                  <  1:649289/251‑1 (MQ=255)                                                                                                                                     ggACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCg                                                                                                <  1:636167/250‑1 (MQ=255)                                                                                                                                       aCGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTc                                                                                                  >  1:474069/1‑246 (MQ=255)                                                                                                                                         gTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGtgttg                                                                                           >  1:643327/1‑251 (MQ=255)                                                                                                                                              aCCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCgg                                                                                                                                         <  1:833560/200‑1 (MQ=255)                                                                                                                                                   aGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCa                                                                                 >  1:851673/1‑251 (MQ=255)                                                                                                                                                          atTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGat                                                                                                                                                                                                                                                    <  1:660160/81‑1 (MQ=255)                                                                                                                                                          atTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCgtgt                                                                                             >  1:91418/1‑232 (MQ=255)                                                                                                                                                            ttACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAATTa                                                                        >  1:101715/1‑251 (MQ=255)                                                                                                                                                                 ggCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCat                                                                                                               <  1:175970/207‑1 (MQ=255)                                                                                                                                                                        cGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTa                                                                                                                                                                                                                                               >  1:820094/1‑72 (MQ=255)                                                                                                                                                                         gTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAATTAACGAAGGCTCGCa                                                           >  1:554853/1‑251 (MQ=255)                                                                                                                                                                          tAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACATCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAAt                                                                          <  1:803490/235‑1 (MQ=255)                                                                                                                                                                          tAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCg                                                                                   <  1:572413/226‑1 (MQ=255)                                                                                                                                                                          tAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAATTAACGAAGGCTCGCaa                                                          >  1:543034/1‑251 (MQ=255)                                                                                                                                                                          tAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAATTAACGAAGGCTCGCaa                                                          >  1:273311/1‑251 (MQ=255)                                                                                                                                                                          tAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAATTAACGAAGGCTCGCaa                                                          >  1:583327/1‑251 (MQ=255)                                                                                                                                                                            ggTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCGAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGAc                                                                                     <  1:84044/222‑1 (MQ=255)                                                                                                                                                                            ggTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGATGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAATTAACGAAGGCTCGCAAAt                                                        >  1:456311/1‑251 (MQ=255)                                                                                                                                                                               aCATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAATTAACGAAgg                                                                 <  1:395093/239‑1 (MQ=255)                                                                                                                                                                                    ggACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGAt                                                                                                                   <  1:374450/184‑1 (MQ=255)                                                                                                                                                                                     gACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAATTAACGAAGGCTCGCAAATCGATCAtt                                                >  1:351639/1‑250 (MQ=255)                                                                                                                                                                                         gCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGt                                                                                                                                        >  1:448199/1‑158 (MQ=255)                                                                                                                                                                                          cTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAATTAACGAAGGCTCGCAAATCGATCATTCCg                                             <  1:893525/248‑1 (MQ=255)                                                                                                                                                                                            tCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAATTAACGAAGGCTCGCAAATCGATCATTCCgtggtg                                        <  1:682402/251‑1 (MQ=255)                                                                                                                                                                                                  aaTCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAATTAACGAAGGCTCGCAAATCGATCATTCCGTGGTGTTACCg                                  <  1:409862/251‑1 (MQ=255)                                                                                                                                                                                                   aTCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAATTAACGAAGGCTCGCAAATCGATCATTCCGTGGTGTTACCg                                  >  1:137141/1‑250 (MQ=255)                                                                                                                                                                                                        atatCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAATTAACGAAGGCTCGCAAAt                                                        >  1:88344/1‑223 (MQ=255)                                                                                                                                                                                                           tCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCTATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAATTAACGAAGGCTCGCAAATCGATCATTCCGTGGTGTTACCGCAAGTGGaa                         <  1:432007/251‑1 (MQ=255)                                                                                                                                                                                                                     ttCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTc                                                                                                  <  1:339580/168‑1 (MQ=255)                                                                                                                                                                                                                     ttCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAAt                                                                          <  1:389419/192‑1 (MQ=255)                                                                                                                                                                                                                               ccGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAATTAACGAAGGCTCGCAAATCGATCATTCCGTGGTGTTACCGCAAGTGGAAATCGGGAAAAACTGCGTGtt     >  1:546363/1‑251 (MQ=255)                                                                                                                                                                                                                                  cAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAATTAACGAAGGCTCGCAAATCGATCATTCCGTGGTGTTACCGCAAGTGGAAATCGGGAAAAACTGCGTGTTAac  <  1:135524/251‑1 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      GATTATCTCTATGAAATGCTGAATCGGGAAGTCAACACGCCTTGCACCTCTCACGACTTTGGTAAAGACGTATTGCCGAAATGTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTCGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAATTAACGAAGGCTCGCAAATCGATCATTCCGTGGTGTTACCGCAAGTGGAAATCGGGAAAAACTGCGTGTTAAC  >  1/1270399‑1270874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑