Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,267,840	G→T	intergenic (+128710/+125471)	unknown → / ← unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,267,840	0	G	T	100.0%	207.4 / NA	69	intergenic (+128710/+125471)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (36/33);  total (36/33)

TTTAGGCGCTGTACACAGCGAAGACATCGCCAGCCATAATCGGGCGAACTCCATTTCCGTCTCTATTCCGCCATTGGCAACCATTTATTTGAAATATCAGGGTAAAGCATGATTGCAGTAAAAAATAACGGTAAACCTTATCCTTTCGGCTACAGCCGATACGAAGAAAGCGACGAACAGATCACGAACTTCGCCCTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGAGGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAATATGGCTTCAGAATCACATCAAAAAATGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACGCAAGCGTGGTTTTTGTTGAGCGACGAGCGGGATAACGCCCGCTA  >  1/1267598‑1268085                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                      tttAGGCGCTGTACACAGCGAAGACATCGCCAGCCATAATCGGGCGAACTCCATTTCCGTCTCTATTCCGCCATTGGCAACCATTTATTTGAAATATCAGGGTAAAGCATGATTGCAGTAAAAAATAACGGTAAACCTTATCCTTTCGGCTACAGCCGATACGAAGAAAGCGACGAACAGATCACGAACTTCTCCCTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGa                                                                                                                                                                                                                                               >  1:139831/1‑251 (MQ=255)      gcgcTGTACACAGCGAAGACATCGCCAGCCATAATCGGGCGAACTCCATTTCCGTCTCTATTCCGCCATTGGCAACCATTTATTTGAAATATCAGGGTAAAGCATGATTGCAGTAAAAAATAACGGTAAACCTTATCCTTTCGGCTACAGCCGATACGAAGAAAGCGACGAACAGATCACGAACTTCTCCCTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATcc                                                                                                                                                                                                                                           >  1:154338/1‑250 (MQ=255)        gcTGTACACAGCGAAGACATCGCCAGCCATAATCGGGCGAACTCCATTTCCGTCTCTATTCCGCCATTGGCAACCATTTATTTGAAATATCAGGGTAAAGCATGATTGCAGTAAAAAATAACGGTAAACCTTATCCTTTCGGCTACAGCCGATACGAAGAAAGCGACGAACAGATCACGAACTTCTCCCTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGt                                                                                                                                                                                                                                        >  1:508666/1‑251 (MQ=255)         cTGTACACAGCGAAGACATCGCCAGCCATAATCGGGCGAACTCCATTTCCGTCTCTATTCCGCCATTGGCAACCATTTATTTGAAATATCAGGGTAAAGCATGATTGCAGTAAAAAATAACGGTAAACCTTATCCTTTCGGCTACAGCCGATACGAAGAAAGCGACGAACAGATCACGAACTTCTCCCTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGtt                                                                                                                                                                                                                                       >  1:670747/1‑251 (MQ=255)           gTACACAGCGAAGACATCGCCAGCCATAATCGGGCGAACTCCATTTCCGTCTCTATTCCGCCATTGGCAACCATTTATTTGAAATATCAGGGTAAAGCATGATTGCAGTAAAAAATAACGGTAAACCTTATCCTTTCGGCTACAGCCGATACGAAGAAAGCGACGAACAGATCACGAACTTCTCCCTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGttt                                                                                                                                                                                                                                      >  1:388937/1‑250 (MQ=255)           gTACACAGCGAAGACATCGCCAGCCATAATCGGGCGAACTCCATTTCCGTCTCTATTCCGCCATTGGCAACCATTTATTTGAAATATCAGGGTAAAGCATGATTGCAGTAAAAAATAACGGTAAACCTTATCCTTTCGGCTACAGCCGATACGAAGAAAGCGACGAACAGATCACGAACTTCTCCCTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGttt                                                                                                                                                                                                                                      >  1:395009/1‑250 (MQ=255)           gTACACAGCGAAGACATCGCCAGCCATAATCGGGCGAACTCCATTTCCGTCTCTATTCCGCCATTGGCAACCATTTATTTGAAATATCAGGGTAAAGCATGATTGCAGTAAAAAATAACGGTAAACCTTATCCTTTCGGCTACAGCCGATACGAAGAAAGCGACGAACAGATCACGAACTTCTCCCTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGttt                                                                                                                                                                                                                                      >  1:568453/1‑250 (MQ=255)                                         cGGGCGAACTCCATTTCCGTCTCTATTCCGCCATTGGCAACCATTTATTTGAAATATCAGGGTAAAGCATGATTGCAGTAAAAAATAACGGTAAACCTTATCCTTTCGGCTACAGCCGATACGAAGAAAGCGACGAACAGATCACGAACTTCTCCCTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCAt                                                                                                                                                                                                       <  1:620070/251‑1 (MQ=255)                                           ggCGAACTCCATTTCCGTCTCTATTCCGCCATTGGCAACCATTTATTTGAAATATCAGGGTAAAGCATGATTGCAGTAAAAAATAACGGTAAACCTTATCCTTTCGGCTACAGCCGATACGAAGAAAGCGACGAACAGATCACGAACTTCTCCCTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGc                                                                                                                                                                                                         <  1:83349/247‑1 (MQ=255)                                            gCGAACTCCATTTCCGTCTCTATTCCGCCATTGGCAACCATTTATTTGAAATATCAGGGTAAAGCATGATTGCAGTAAAAAATAACGGTAAACCTTATCCTTTCGGCTACAGCCGATACGAAGAAAGCGACGAACAGATCACGAACTTCTCCCTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCAttt                                                                                                                                                                                                     <  1:702910/250‑1 (MQ=255)                                                 cTCCATTTCCGTCTCTATTCCGCCATTGGCAACCATTTATTTGAAATATCAGGGTAAAGCATGATTGCAGTAAAAAATAACGGTAAACCTTATCCTTTCGGCTACAGCCGATACGAAGAAAGCGACGAACAGATCACGAACTTCTCCCTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCAt                                                                                                                                                                                                       >  1:883762/1‑243 (MQ=255)                                                 cTCCATTTCCGTCTCTATTCCGCCATTGGCAACCATTTATTTGAAATATCAGGGTAAAGCATGATTGCAGTAAAAAATAACGGTAAACCTTATCCTTTCGGCTACAGCCGATACGAAGAAAGCGACGAACAGATCACGAACTTCTCCCTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGtt                                                                                                                                                                                               >  1:871046/1‑251 (MQ=255)                                                   ccATTTCCGTCTCTATTCCGCCATTGGCAACCATTTATTTGAAATATCAGGGTAAAGCATGATTGCAGTAAAAAATAACGGTAAACCTTATCCTTTCGGCTACAGCCGATACGAAGAAAGCGACGAACAGATCACGAACTTCTCCCTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTaa                                                                                                                                                                                             >  1:807066/1‑251 (MQ=255)                                                   ccATTTCCGTCTCTATTCCGCCATTGGCAACCATTTATTTGAAATATCAGGGTAAAGCATGATTGCAGTAAAAAATAACGGTAAACCTTATCCTTTCGGCTACAGCCGATACGAAGAAAGCGACGAACAGATCACGAACTTCTCCCTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTaa                                                                                                                                                                                             >  1:279569/1‑251 (MQ=255)                                                            tctcTATTCCGCCATTGGCAACCATTTATTTGAAATATCAGGGTAAAGCATGATTGCAGTAAAAAATAACGGTAAACCTTATCCTTTCGGCTACAGCCGATACGAAGAAAGCGACGAACAGATCACGAACTTCTCCCTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTgg                                                                                                                                                                                    >  1:781933/1‑251 (MQ=255)                                                               ctATTCCGCCATTGGCAACCATTTATTTGAAATATCAGGGTAAAGCATGATTGCAGTAAAAAATAACGGTAAACCTTATCCTTTCGGCTACAGCCGATACGAAGAAAGCGACGAACAGATCACGAACTTCTCCCTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGc                                                                                                                                                                                           >  1:260601/1‑241 (MQ=255)                                                                        cATTGGCAACCATTTATTTGAAATATCAGGGTAAAGCATGATTGCAGTAAAAAATAACGGTAAACCTTATCCTTTCGGCTACAGCCGATACGAAGAAAGCGACGAACAGATCACGAACTTCTCCCTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAAccc                                                                                                                                                                        <  1:376565/251‑1 (MQ=255)                                                                                          tGAAATATCAGGGTAAAGCATGATTGCAGTAAAAAATAACGGTAAACCTTATCCTTTCGGCTACAGCCGATACGAAGAAAGTGACGAACAGATCACGAACTTCTCCCTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAATATGGCTTCAGAATca                                                                                                                                                      <  1:453721/251‑1 (MQ=255)                                                                                             aaTATCAGGGTAAAGCATGATTGCAGTAAAAAATAACGGTAAACCTTATCCTTTCGGCTACAGCCGATACGAAGAAAGCGACGAACAGATCACGAACTTCTCCCTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAATATGGCTTCAGAATCACAt                                                                                                                                                   <  1:743189/251‑1 (MQ=255)                                                                                               tatCAGGGTAAAGCATGATTGCAGTAAAAAATAACGGTAAACCTTATCCTTTCGGCTACAGCCGATACGAAGAAAGCGACGAACAGATCACGAACTTCTCCCTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAAc                                                                                                                                                                                                                                  >  1:693151/1‑170 (MQ=255)                                                                                               tatCAGGGTAAAGCATGATTGCAGTAAAAAATAACGGTAAACCTTATCCTTTCGGCTACAGCCGATACGAAGAAAGCGACGAACAGATCACGAACTTCTCCCTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAATATGGCTTCAGAATCACATCa                                                                                                                                                 >  1:275613/1‑251 (MQ=255)                                                                                                    gggTAAAGCATGATTGCAGTAAAAAATAACGGTAAACCTTATCCTTTCGGCTACAGCCGATACGAAGAAAGCGACGAACAGATCACGAACTTCTCCCTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTc                                                                                                                                                                                                                       <  1:561263/176‑1 (MQ=255)                                                                                                    gggTAAAGCATGATTGCAGTAAAAAATAACGGTAAACCTTATCCTTTCGGCTACAGCCGATACGAAGAAAGCGACGAACAGATCACGAACTTCTCCCTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCa                                                                                                                                                                                                        >  1:306314/1‑191 (MQ=255)                                                                                                    gggTAAAGCATGATTGCAGTAAAAAATAACGGTAAACCTTATCCTTTCGGCTACAGCCGATACGAAGAAAGCGACGAACAGATCACGAACTTCTCCCTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGc                                                                                                                                                                                           >  1:585039/1‑204 (MQ=255)                                                                                                    gggTAAAGCATGATTGCAGTAAAAAATAACGGTAAACCTTATCCTTTCGGCTACAGCCGATACGAAGAAAGCGACGAACAGATCACGAACTTCTCCCTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAATATGGCTTCAGAATCACATCaaaaaa                                                                                                                                            >  1:590918/1‑251 (MQ=255)                                                                                                    gggTAAAGCATGATTGCAGTAAAAAATAACGGTAAACCTTATCCTTTCGGCTACAGCCGATACGAAGAAAGCGACGAACAGATCACGAACTTCTCCCTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAATATGGCTTCAGAATCACATCaaaaaa                                                                                                                                            >  1:12819/1‑251 (MQ=255)                                                                                                           gCATGATTGCAGTAAAAAATAACGGTAAACCTTATCCTTTCGGCTACAGCCGATACGAAGAAAGCGACGAACAGATCACGAACTTCTCCCTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAATATGGCTTCAGAATCACATCAAAAAATGACCg                                                                                                                                      >  1:364698/1‑250 (MQ=255)                                                                                                             aTGATTGCAGTAAAAAATAACGGTAAACCTTATCCTTTCGGCTACAGCCGATACGAAGAAAGCGACGAACAGATCACGAACTTCTCCCTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAATATGGCTTCAGAATCACATCAAAAAATGACCGCAc                                                                                                                                   <  1:593563/251‑1 (MQ=255)                                                                                                                    cAGTAAAAAATAACGGTAAACCTTATCCTTTCGGCTACAGCCGATACGAAGAAAGCGACGAACAGATCACGAACTTCTCCCTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAATATGGCTTCAGAATCACATCAAAAAATGACCGCACTTTGAc                                                                                                                             <  1:510373/250‑1 (MQ=255)                                                                                                                    cAGTAAAAAATAACGGTAAACCTTATCCTTTCGGCTACAGCCGATACGAAGAAAGCGACGAACAGATCACGAACTTCTCCCTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAATATGGCTTCAGAATCACATCAAAAAATGACCGCACTTTGAc                                                                                                                             <  1:762519/250‑1 (MQ=255)                                                                                                                      gTAAAAAATAACGGTAAACCTTATCCTTTCGGCTACAGCCGATACGAAGAAAGCGATGAACAGATCACGAACTTCTCCCTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAATATGGCTTCAGAATCACAt                                                                                                                                                   <  1:436116/226‑1 (MQ=255)                                                                                                                       tAAAAAATAACGGTAAACCTTATCCTTTCGGCTACAGCCGATACGAAGAAAGCGACGAACAGATCACGAACTTCTCCCTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAATATGGCTTCAGAATCACATCAAAAAATGACCGCACTTTGACgg                                                                                                                           <  1:763880/249‑1 (MQ=255)                                                                                                                           aaaTAACGGTAAACCTTATCCTTTCGGCTACAGCCGATACGAAGAAAGCGACGAACAGATCACGAACTTCTCCCTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTc                                                                                                                                                                                                                       >  1:277511/1‑153 (MQ=255)                                                                                                                           aaaTAACGGTAAACCTTATCCTTTCGGCTACAGCCGATACGAAGAAAGCGACGAACAGATCACGAACTTCTCCCTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAATATGGc                                                                                                                                                                <  1:625855/208‑1 (MQ=255)                                                                                                                                 cGGTAAACCTTATCCTTTCGGCTACAGCCGATACGAAGAAAGCGACGAACAGATCACGAACTTCTCCCTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAATATGGCTTCAGAATCACATCAAAAAATGACCGCACTTTGACGGCAAATCCGCAGa                                                                                                               >  1:889031/1‑251 (MQ=255)                                                                                                                                       aCCTTATCCTTTCGGCTACAGCCGATACGAAGAAAGCGACGAACAGATCACGAACTTCTCCCTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACa                                                                                                                                                                                                                  <  1:809957/146‑1 (MQ=255)                                                                                                                                       aCCTTATCCTTTCGGCTACAGCCGATACGAAGAAAGCGACGAACAGATCACGAACTTCTCCCTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCaa                                                                                                                                                                                 <  1:198690/179‑1 (MQ=255)                                                                                                                                       aCCTTATCCTTTCGGCTACAGCCGATACGAAGAAAGCGACGAACAGATCACGAACTTCTCCCTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAatat                                                                                                                                                                   <  1:746532/193‑1 (MQ=255)                                                                                                                                       aCCTTATCCTTTCGGCTACAGCCGATACGAAGAAAGCGACGAACAGATCACGAACTTCTCCCTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAATATGGCTTCAGAATCACATCAAAAAATGACCGCACTTTGACGGCAAATCCGCAGAAATTGa                                                                                                         >  1:538713/1‑251 (MQ=255)                                                                                                                                          ttATCCTTTCGGCTACAGCCGATACGAAGAAAGCGACGAACAGATCACGAACTTCTCCCTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAATATGGCTTCAGAATCACATCAAAAAATGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGc                                                                                                      <  1:527447/251‑1 (MQ=255)                                                                                                                                              ccTTTCGGCTACAGCCGATACGAAGAAAGCGACGAACAGATCACGAACTTCTCCCTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAATATGGCTTCAGAATCACATCAAAAAATGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGCTGGa                                                                                                  >  1:157771/1‑251 (MQ=255)                                                                                                                                               cTTTCGGCTACAGCCGATACGAAGAAAGCGACGAACAGATCACGAACTTCTCCCTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAATATGGCTTCAGAATCACATCAAAAAATGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGCTGGAc                                                                                                 <  1:217729/251‑1 (MQ=255)                                                                                                                                                    ggCTACAGCCGATACGAAGAAAGCGACGAACAGATCACGAACTTCTCCCTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAatat                                                                                                                                                                   <  1:154599/180‑1 (MQ=255)                                                                                                                                                     gCTACAGCCGATACGAAGAAAGCGACGAACAGATCACGAACTTCTCCCTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAATATGGCTTCAGAATCACATCAAAAAATGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTa                                                                                            <  1:794446/250‑1 (MQ=255)                                                                                                                                                       tACAGCCGATACGAAGAAAGCGACGAACAGATCACGAACTTCTCCCTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAATATGGCTTCAGAATCACATCAAAAAATGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTATGc                                                                                         <  1:14577/251‑1 (MQ=255)                                                                                                                                                       tACAGCCGATACGAAGAAAGCGACGAACAGATCACGAACTTCTCCCTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAATATGGCTTCAGAATCACATCAAAAAATGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTATGc                                                                                         <  1:280128/251‑1 (MQ=255)                                                                                                                                                                  cGAAGAAAGCGACGAACAGATCACGAACTTCTCCCTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAATATGGCTTCAGAAt                                                                                                                                                        <  1:46240/177‑1 (MQ=255)                                                                                                                                                                             acgaACAGATCACGAACTTCTCCCTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAATATGGCTTCAGAATCACATCAAAAAATGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAAcc                                                                    <  1:865039/250‑1 (MQ=255)                                                                                                                                                                             acgaACAGATCACGAACTTCTCCCTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAATATGGCTTCAGAATCACATCAAAAAATGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCg                                                                   >  1:846373/1‑251 (MQ=255)                                                                                                                                                                                  cAGATCACGAACTTCTCCCTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAatat                                                                                                                                                                   <  1:389727/150‑1 (MQ=255)                                                                                                                                                                                    gATCACGAACTTCTCCCTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGc                                                                                                                                                                                           <  1:534669/124‑1 (MQ=255)                                                                                                                                                                                    gATCACGAACTTCTCCCTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAAccc                                                                                                                                                                        <  1:311961/143‑1 (MQ=255)                                                                                                                                                                                    gATCACGAACTTCTCCCTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAATATGGCTTCAGAATCACATCaaaaaa                                                                                                                                            >  1:15566/1‑171 (MQ=255)                                                                                                                                                                                    gATCACGAACTTCTCCCTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAATATGGCTTCAGAATCACATCAAAAAATGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTaa                                                            >  1:667133/1‑251 (MQ=255)                                                                                                                                                                                    gATCACGAACTTCTCCCTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAATATGGCTTCAGAATCACATCAAAAAATGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTaa                                                            >  1:779806/1‑251 (MQ=255)                                                                                                                                                                                      tCACGAACTTCTCCCTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAATATGGCTTCAGAATCACATCAAAAAATGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGc                                                          <  1:122765/251‑1 (MQ=255)                                                                                                                                                                                             cTTCTCCCTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTc                                                                                                                                                                                                                       >  1:692676/1‑87 (MQ=255)                                                                                                                                                                                               tctcccTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAATATGGCTTCAGAATCACATCAAAAAATGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGaa                                                 >  1:589820/4‑251 (MQ=255)                                                                                                                                                                                                ctcccTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAATATg                                                                                                                                                                  >  1:281141/3‑137 (MQ=255)                                                                                                                                                                                                  cccTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAATATGGCTTCAGAATCACATCAAAAAATGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACg                                              >  1:459066/1‑251 (MQ=255)                                                                                                                                                                                                  cccTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAATATGGCTTCAGAATCACATCAAAAAATGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACg                                              >  1:326599/1‑251 (MQ=255)                                                                                                                                                                                                    cTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAATATGGCTTCAGAATCACATCAAAAAATGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCgtgg                                                                              >  1:897627/1‑217 (MQ=255)                                                                                                                                                                                                      gTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAATATGGCTTCAGAAt                                                                                                                                                        >  1:809130/1‑141 (MQ=255)                                                                                                                                                                                                        aTTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCAt                                                                                                                                                                                                       <  1:77737/92‑1 (MQ=255)                                                                                                                                                                                                           cttcGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAATATGGCTTCAGAATCACATCAAAAAATGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGc                                                                         <  1:759480/215‑1 (MQ=255)                                                                                                                                                                                                                   gcgcAACAGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAATATGGCTTCAGAATCACATCAAAAAATGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACGCAAGCGTGGTTTttgt                              <  1:50260/250‑1 (MQ=255)                                                                                                                                                                                                                          aGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAATATGGCTTCAGAATCACATCAAAAAATGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGc                                                                                                      <  1:477463/171‑1 (MQ=255)                                                                                                                                                                                                                          aGGTGGAACTTTGTCTGTTTGAAGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAATATGGCTTCAGAATCACATCAAAAAATGACCGCACTTTGACGGCAAATCCGCAGAAATTGATACTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACGCAAGCGTGGTTTTTGTTGAGcga                       >  1:422975/1‑250 (MQ=255)                                                                                                                                                                                                                                               aaGATGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAATATGGCTTCAGAATCACATCAAAAAATGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACGCAAGCGTGGTTTTTGTTGAGCGACGAGCGGGATAACGCCCGCTa  <  1:105822/250‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                      TTTAGGCGCTGTACACAGCGAAGACATCGCCAGCCATAATCGGGCGAACTCCATTTCCGTCTCTATTCCGCCATTGGCAACCATTTATTTGAAATATCAGGGTAAAGCATGATTGCAGTAAAAAATAACGGTAAACCTTATCCTTTCGGCTACAGCCGATACGAAGAAAGCGACGAACAGATCACGAACTTCGCCCTGTATTCTTCGGCGGCGCAACAGGTGGAACTTTGTCTGTTTGAAGAGGAGCGGGAATCCCGTTTCAACATGGTTAATTCCGACAACGTGTGGCATTTGGCGTTAAGCGGGGTGGCAAACGGAACCCAATATGGCTTCAGAATCACATCAAAAAATGACCGCACTTTGACGGCAAATCCGCAGAAATTGATGCTGGACCCTTATGCCAAGGCCGTGGTGGGCAAACCGGATTTAAGCTCGCCTGAAACGCAAGCGTGGTTTTTGTTGAGCGACGAGCGGGATAACGCCCGCTA  >  1/1267598‑1268085

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑