Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,261,065	G→T	intergenic (+121935/+132246)	unknown → / ← unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,261,065	0	G	T	100.0%	194.5 / NA	72	intergenic (+121935/+132246)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (28/44);  total (28/44)

GCCATTTATCGCCCAATCCCAGTTTTTCCAATAATTGTTTGGCACGTTCCAAGCGTTGGCTTTGTGGCATTCCCGCATAAATTGCAGGTAAGGCGACGTTTTCTGCGGCGGTTAAGCTGCTTAATAGGTTATAGCGTTGGAAAATAAAACCGAATTTTTGGCTGCGTAAATCGGAAAGCTGATCGTTGGTTAATTCAATGGTTTCTTTTCCATCAATTTTGGATGAACCACCGGTTGCCGTGTCCAAACAACCAATGATATTCATCAGTGTGGATTTGCCTGAACCAGATTGTCCCATAATGGCGACAAAATCACCTCTTTCGATGGAAAGGGAAATGTCTTTTAAAACATGAACGCGGTTTTCACCTTCGCCAAAATAACGGTTGAGCTGCTTGATTTCAATTATATTCATCGTCGAAACCCACCGCACTTTAGAAGATAATCGGGGCATCAGGAT  >  1/1260844‑1261300                                                                                                                                                                                                                              |                                                                                                                                                                                                                                            gCCATTTATCGCCCAATCCCAGTTTTTCCAATAATTGTTTGGCACGTTCCAAGCGTTGGCTTTGTGGCATTCCCGCATAAATTGCAGGTAAGGCGACGTTTTCTGCGGCGGTTAAGCTGCTTAATAGGGTATAGCGTTGGAAAAAAAAAACGAATTTTTGGCTGCGTAAATCGGAAAACTGATCGTTGGTTAATTCAATGGGTTTTTTTTCATCAATTTTGTATGAACCACCGGTTGCCGTGTCCAaacaa                                                                                                                                                                                                                >  1:336169/1‑251 (MQ=255)   cATTTATCGCCCAATCCCAGTTTTTCCAATAATTGTTTGGCACGTTCCAAGCGTTGGCTTTGTGGCATTCCCGCATAAATTGCAGGTAAGGCGACGTTTTCTGCGGCGGTTAAGCTGCTTAATAGGTTATAGCGTTGGAAAATAAAACCGAATTTTTGGCTGCGTAAATCGGAAAGCTGATCGTTGGTTAATTCAATGGTTTCTTTTCCATCAATTTTGTATGAACCACCGGTTGCCGTGTCCAAACAAcc                                                                                                                                                                                                              <  1:273081/251‑1 (MQ=255)       tATCGCCCAATCCCAGTTTTTCCAATAATTGTTTGGCACGTTCCAAGCGTTGGCTTTGTGGCATTCCCGCATAAATTGCAGGTAAGGCGACGTTTTCTGCGGCGGTTAAGCTGCTTAATAGGTTATAGCGTTGGAAAATAAAACCGAATTTTTGGCTGCGTAAATCGGAAAGCTGATCGTTGGTTAATTCAATGGTTTCTTTTCCATCAATTTTGTATGAACCACCGGTTGCCGTGTCCAAACAACCAATg                                                                                                                                                                                                          <  1:67179/251‑1 (MQ=255)         tCGCCCAATCCCAGTTTTTCCAATAATTGTTTGGCACGTTCCAAGCGTTGGCTTTGTGGCATTCCCGCATAAATTGCAGGTAAGGCGACGTTTTCTGCGGCGGTTAAGCTGCTTAATAGGTTATAGCGTTGGAAAATAAAACCGAATTTTTGGCTGCGTAAATCGGAAAGCTGATCGTTGGTTAATTCAATGGTTTCTTTTCCATCAATTTTGTATGAACCACCGGTTGCCGTGTCCAAACAACCAATGat                                                                                                                                                                                                        <  1:164755/251‑1 (MQ=255)         tCGCCCAATCCCAGTTTTTCCAATAATTGTTTGGCACGTTCCAAGCGTTGGCTTTGTGGCATTCCCGCATAAATTGCAGGTAAGGCGACGTTTTCTGCGGCGGTTAAGCTGCTTAATAGGTTATAGCGTTGGAAAATAAAACCGAATTTTTGGCTGCGTAAATCGGAAAGCTGATCGTTGGTTAATTCAATGGTTTCTTTTCCATCAATTTTGTATGAACCACCGGTTGCCGTGTCCAAACAACCAATGat                                                                                                                                                                                                        <  1:712034/251‑1 (MQ=255)            cccAATCCCAGTTTTTCCAATAATTGTTTGGCACGTTCCAAGCGTTGGCTTTGTGGCATTCCCGCATAAATTGCAGGTAAGGCGACGTTTTCTGCGGCGGTTAAGCTGCTTAATAGGTTATAGCGTTGGAAAATAAAACCGAATTTTTGGCTGCGTAAATCGGAAAGCTGATCGTTGGTTAATTCAATGGTTTCTTTTCCATCAATTTTGTATGAACCACCGGTTGCCGTGTCCAAACAACCAATGatat                                                                                                                                                                                                      <  1:325903/250‑1 (MQ=255)                      gTTTTTCCAATAATTGTTTGGCACGTTCCAAGCGTTGGCTTTGTGGCATTCCCGCATAAATTGCAGGTAAGGCGACGTTTTCTGCGGCGGTTAAGCTGCTTAATAGGTTATAGCGTTGGGAAATAAAACCGAATTTTTTGCTGCGTAAATCGGGAAGCTGATCGTTGGGTAATTCAATGGTTTTTTTTTCCTCAATTTTGTATGAacc                                                                                                                                                                                                                                      >  1:117384/1‑208 (MQ=255)                       tttttCCAATAATTGTTTGGCACGTTCCAAGCGTTGGCTTTGTGGCATTCCCGCATAAATTGCAGGTAAGGCGACGTTTTCTGCGGCGGTTAAGCTGCTTAATAGGTTATAGCGTTGGAAAATAAAACCGAATTTTTGGCTGCGTAAATCGGAAAGCTGATCGTTGGTTAATTCAATGGTTTCTTTTCCATCAATTTTGTATGAACCACCGGTTGCCGTGTCCAAACAACCAATGATATTCATCAgtg                                                                                                                                                                                             <  1:303553/248‑1 (MQ=255)                          ttCCAATAATTGTTTGGCACGTTCCAAGCGTTGGCTTTGTGGCATTCCCGCATAAATTGCAGGTAAGGCGACGTTTTCTGCGGCGGTTAAGCTGCTTAATAGGTTATAGCGTTGGAAAATAAAACCGAATTTTTGGCTGCGTAAATCGGAAAGCTGATCGTTGGTTAATTCAATGGTTTCTTTTCCATCAATTTTGTATGAACCACCGGTTGCCGTGTCCAAACAACCAATGATATTCATCAGTGTGGAtt                                                                                                                                                                                       <  1:412291/251‑1 (MQ=255)                          ttCCAATAATTGTTTGGCACGTTCCAAGCGTTGGCTTTGTGGCATTCCCGCATAAATTGCAGGTAAGGCGACGTTTTCTGCGGCGGTTAAGCTGCTTAATAGGTTATAGCGTTGGAAAATAAAACCGAATTTTTGGCTGCGTAAATCGGAAAGCTGATCGTTGGTTAATTCAATGGTTTCTTTTCCATCAATTTTGTATGAACCACCGGTTGCCGTGTCCAAACAACCAATGATATTCATCAGTGTGGAtt                                                                                                                                                                                       <  1:885686/251‑1 (MQ=255)                              aataatTGTTTGGCACGTTCCAAGCGTTGGCTTTGTGGCATTCCCGCATAAATTGCAGGTAAGGCGACGTTTTCTGCGGCGGTTTAGCTGCTTAATAGGGTATAACGTTGGAAAATAAAACCGAATTTTTTGCTGCGTAAATCGGAAAGGTGATCGTTGGTTAATTCAATGGTTTCTTTTCCATCAATTTTTTATGAACCACCGGGTGCCGTGTCCAAACAAACAAAGATATTTATCAGTGTGGGTTTGc                                                                                                                                                                                    >  1:180388/1‑250 (MQ=255)                              aataatTGTTTGGCACGTTCCAAGCGTTGGCTTTGTGGCATTCCCGCATAAATTGCAGGTAAGGCGACGTTTTCTGCGGCGGTTAAGCTGCTTAATAGGTTATAGCGTTGGAAAATAAAACCGAATTTTTGGCTGCGTAAATCGGAAAGCTGATCGTTGGTTAATTCAATGGTTTCTTTTCCATCAATTTTGTATGAACCACCGGTTGCCGTGTCCAAACAACCAATGATATTCATCAGTGTGGATTTGcc                                                                                                                                                                                   <  1:117375/251‑1 (MQ=255)                                    tGTTTGGCACGTTCCAAGCGTTGGCTTTGTGGCATTCCCGCATAAATTGCAGGTAAGGCGACGTTTTCTGCGGCGGTTAAGCTGCTTAATAGGTTATAGCGTTGGAAAATAAAACCGAATTTTTGGCTGCGTAAATCGGAAAGCTGATCGTTGGTTAATTCAATGGTTTCTTTTCCATCAATTTTGTATGAACCACCGGTTGCCGTGTCCAAACAACCAATGATATTCATCAGTGTGGATTTGCCTGAAcc                                                                                                                                                                             <  1:595828/251‑1 (MQ=255)                                     gTTTGGCACGTTCCAAGCGTTGGCTTTGTGGCATTCCCGCATAAATTGCAGGTAAGGCGACGTTTTCTGCGGCGGTTAAGCTGCTTAATAGGTTATAGCGTTGGAAAATAAAACCGAATTTTTGGCTGCGTAAATCGGAAAGCTGATCGTTGGTTAATTCAATGGTTTCTTTTCCATCAATTTTGTATGAACCACCGGTTGCCGTGTCCAAACAACCAATGATATtcatc                                                                                                                                                                                                 <  1:553608/230‑1 (MQ=255)                                     gTTTGGCACGTTCCAAGCGTTGGCTTTGTGGCATTCCCGCATAAATTGCAGGTAAGGCGACGTTTTCTGCGGCGGTTAAGCTGCTTAATAGGTTATAGCGTTGGAAAATAAAACCGAATTTTTGGCTGCGTAAATCGGAAAGCTGATCGTTGGTTAATTCAATGGTTTCTTTTCCATCAATTTTGTATGAACCACCGGTTGCCGTGTCCAAACAACCAATGATATTCATCAgtg                                                                                                                                                                                             <  1:356281/234‑1 (MQ=255)                                        tGGCACGTTCCAAGCGTTGGCTTTGTGGCATTCCCGCATAAATTGCAGGTAAGGCGACGTTTTCTGCGGCGGTTAAGCTGCTTAATAGGTTATAGCGTTGGAAAATAAAACCGAATTTTTGGCTGCGTAAATCGGAAAGCTGATCGTTGGTTAATTCAATGGTTTCTTTTCCATCAATTTTGTATGAACCACCGGTTGCCGTGTCCAAACAACCAATGATATTCATCAGTGTGGATTTGCCTGAACCAGAt                                                                                                                                                                         <  1:399398/251‑1 (MQ=255)                                             cGTTCCAAGCGTTGGCTTTGTGGCATTCCCGCATAAATTGCAGGTAAGGCGACGTTTTCTGCGGCGGTTAAACTGCTTTATAGGTTATAGCGTTGGAAAATAAAACCGAATTTTTTGCTGCGTAAATTGGAAAGCTGGTCCTTGGGTTATTCAATGGGTTTTTTTTCATCAATTTTTTATGAAACACCCGTTGCCCGGTCCCAACAAACAATGATATTCATTATTTTGGCTTTTCCTGAACCAGAttttc                                                                                                                                                                     >  1:676888/1‑247 (MQ=255)                                              gTTCCAAGCGTTGGCTTTGTGGCATTCCCGCATAAATTGCAGGTAAGGCGACGTTTTCTGCGGCGGTTAAGCTGCTTAATAAGTTATAGCGGTGGGAAATAAAAACGAAATTTTGGCTGCGTAAATCGGAAAGCTGATCGTTGGGTAATTCAATGGGTTCTTTTTCATCAATTTTGTATTAACCACCCGTTGCCGTGTCCAAACAACCAATGATATTCATCAGTGTGGATTTTCCTGAACCAGAt                                                                                                                                                                         >  1:90230/1‑245 (MQ=255)                                               ttCCAAGCGTTGGCTTTGTGGCATTCCCGCATAAATTGCAGGTAAGGCGACGTTTTCTGCGGCGGTTAAGCTGCTTTATAGGGTATAGCGTTGGGAAATAAAACCGAATTTTTTGCTGCGTAAATCGGAAAGCTGATCGTTGGTTAATTCAATGGTTTCTTTTTCATCAATTTTGTATGAAACACCGGTTGCCGTGTCCAAACAACCAATGATATTTATCAGTGTGGATTTTCCTGGAcccgaatttccc                                                                                                                                                                   >  1:841142/1‑240 (MQ=255)                                               ttCCAAGCGTTGGCTTTGTGGCATTCCCGCATAAATTGCAGGTAAGGCGACGTTTTCTGCGGCGGTTAAGCTGCTTAATAGGTTATAGCGTTGGAAAATAAAACCGAATTTTTGGCTGCGTAAATCGGAAAGCTGATCGTTGGTTAATTCAATGGTTTCTTTTCCATCAATTTTGTATGAACCACCGGTTGCCGTGTCCAAACAACCAATGATATtcatc                                                                                                                                                                                                 <  1:64518/220‑1 (MQ=255)                                               ttCCAAGCGTTGGCTTTGTGGCATTCCCGCATAAATTGCAGGTAAGGCGACGTTTTCTGCGGCGGTTAAGCTGCTTAATAGGGTATAGCGTTGGGAAATAAAACCGAATTTTTTGCTGCGTAAATCGGAAAGCTGATCGTTGGTTAATTCAATGGGTTCTTTTCCATCAATTTTGTATGAACCCCCCGTTTCCGTGTCCAAACAACCAATGATATTCATCAGTGTGGGTTTTCCTGAACCAGATTGTccc                                                                                                                                                                   >  1:283838/1‑250 (MQ=255)                                               ttCCAAGCGTTGGCTTTGTGGCATTCCCGCATAAATTGCAGGTAAGGCGACGTTTTCTGCGGCGGTTAAGCTGCTTAATAGGGTATAGCGTTGGGAAATAAAACCGAATTTTTGGCTGCGTAAATCGGAAAGCTGATCGTTGGTTAATTCAATGGTTTCTTTTCCATCAATTTTTTATTAACCACCGGTTGCCGTGTCCAAACAACCAATGATATTCATCAGTGTGGATTTTCCTGAACCAGATTGTccc                                                                                                                                                                   >  1:80187/1‑250 (MQ=255)                                                  cAAGCGTTGGCTTTGTGGCATTCCCGCATAAATTGCAGGTAAGGCGACGTTTTCTGCGGCGGTTAAGCTGCTTAATAGGTTATAGCGTTGGAAAATAAAACCGAATTTTTGGCTGCGTAAATCGGAAAGCTGATCGTTGGTTAATTCAATGGTTTCTTTTCCATCAATTTTGTATGAACCACCGGTTGCCGTGTCCAAACAACCAATGATATTCATCAGTGTGGATTTGCCTGAACCAGATTGTCCCATaa                                                                                                                                                               <  1:821396/251‑1 (MQ=255)                                                  cAAGCGTTGGCTTTGTGGCATTCCCGCATAAATTGCAGGTAAGGCGACGTTTTCTGCGGCGGTTAAGCTGCTTAATAGGTTATAGCGTTGGAAAATAAAACCGAATTTTTGGCTGCGTAAATCGGAAAGCTGATCGTTGGTTAATTCAATGGTTTCTTTTCCATCAATTTTGTATGAACCACCGGTTGCCGTGTCCAAACAACCAATGATATTCATCAGTGTGGATTTGCCTGAACCAGATTGTCCCATaa                                                                                                                                                               <  1:606474/251‑1 (MQ=255)                                                  cAAGCGTTGGCTTTGTGGCATTCCCGCATAAATTGCAGGTAAGGCGACGTTTTCTGCGGCGGTTAAGCTGCTTAATAGGTTATAGCGTTGGAAAATAAAACCGAATTTTTGGCTGCGTAAATCGGAAAGCTGATCGTTGGTTAATTCAATGGTTTCTTTTCCATCAATTTTGTATGAACCACCGGTTGCCGTGTCCAAACAACCAATGATATTCATCAGTGTGGATTTGCCTGAACCAGATTGTCCCATaa                                                                                                                                                               <  1:527349/251‑1 (MQ=255)                                                  cAAGCGTTGGCTTTGTGGCATTCCCGCATAAATTGCAGGTAAGGCGACGTTTTCTGCGGCGGTTAAGCTGCTTAATAGGTTATAGCGTTGGAAAATAAAACCGAATTTTTGGCTGCGTAAATCGGAAAGCTGATCGTTGGTTAATTCAATGGTTTCTTTTCCATCAATTTTGTATGAACCACCGGTTGCCGTGTCCAAACAACCAATGATATTCATCAGTGTGGATTTGCCTGAACCAGATTGTCCCATaa                                                                                                                                                               <  1:129990/251‑1 (MQ=255)                                                   aaGCGTTGGCTTTGTGGCATTCCCGCATAAATTGCAGGTAAGGCGACGTTTTCTGCGGCGGTTAAGCTGCTTAATAGGTTATAGCGTTGGAAAATAAAACCGAATTTTTGGCTGCGTAAATCGGAAAGCTGATCGTTGGTTAATTCAATGGTTTCTTTTCCATCAATTTTGTATGAACCACCGGTTGCCGTGTCCAAACAACCAATGATATTCATCAGTGTGGATTTGCCTGAACCAGATTGTCCCATAAt                                                                                                                                                              <  1:513855/251‑1 (MQ=255)                                                           gCTTTGTGGCATTCCCGCATAAATTGCAGGTAAGGCGACGTTTTCTGCGGCGGTTAAGCTGCTTAATAGGTTATAGCGTTGGAAAATAAAACCGAATTTTTTGCTGCGTAAATCGGAAAGCTGATCGTTGGTTAATTCAATGGGTTCTTTTTCATCAATTTTGTATGAACCACCGGGTGCCGTGTCCAAACAACCAATGATATTCATCAGTGTGGATTTTCCTGAACCAGATTGTCCCAAAATGGCGACaa                                                                                                                                                      >  1:493030/1‑251 (MQ=255)                                                           gCTTTGTGGCATTCCCGCATAAATTGCAGGTAAGGCGACGTTTTCTGCGGCGGTTAAGCTGCTTAATAGGTTATAGCGTTGGAAAATAAAACCGAATTTTTGGCTGCGTAAATCGGAAAGCTGATCGTTGGTTAATTCAATGGTTTCTTTTCCATCAATTTTGTATGAACCACCGGTTGCCGTGTCCAAACAACCAATGATATTCATCAGTGTGGATTTGCCTGAAcc                                                                                                                                                                             <  1:460731/228‑1 (MQ=255)                                                              ttGTGGCATTCCCGCATAAATTGCAGGTAAGGCGACGTTTTCTGCGGCGGTTAAGCTGCTTAATAGGTTATAGCGTTGGAAAATAAAACCGAATTTTTGGCTGCGTAAATCGGAAAGCTGATCGTTGGTTAATTCAATGGTTTCTTTTCCATCAATTTTGTATGAACCACCGGTTGCCGTGTCCAAACAACCAATGATATTCATCAGTGTGGATTTGCCTGAACCAGATTGTCCCATAATGGCGACAAAAt                                                                                                                                                   <  1:308979/251‑1 (MQ=255)                                                                    cATTCCCGCATAAATTGCAGGTAAGGCGACGTTTTCTGCGGCGGTTAAGCTGCTTAATAGGTTATAGCGTTGGAAAATAAAACCGAATTTTTGGCTGCGTAAATCGGAAAGCTGATCGTTGGTTAATTCAATGGTTTCTTTTCCATCAATTTTGTATGAACCACCGGTTGCCGTGTCCAAACAACCAATGATATTCATCAGTGTGGATTTGCCTGAACCAGATTGTCCCATAATGGCGACAAAATCACctc                                                                                                                                             <  1:782125/251‑1 (MQ=255)                                                                         ccGCATAAATTGCAGGTAAGGCGACGTTTTCTGCGGCGGTTAAGCTGCTTAATAGGTTATAGCGTTGGAAAATAAAACCGAATTTTTGGCTGCGTAAATCGGAAAGCTGATCGTTGGTTAATTCAATGGTTTCTTTTCCATCAATTTTGTATGAACCACCGGTTGCCGTGTCCAAACAACCAATGATATTCATCAGTGTGGATTTGCCTGAACCAGATTGTCCCATAATGGCGACAAAATCACCTCTTTCg                                                                                                                                        <  1:689881/251‑1 (MQ=255)                                                                          cGCATAAATTGCAGGTAAGGCGACGTTTTCTGCGGCGGTTAAGCTGCTTAATAGGGTATAGCGTTGGGAAATAAAACCGAATTTTTTGCTGCGTAAATCGGAAAGCTGATCGTTGGGTAATTTAATGGGTTCTTTTTCATCAATTTTGTATGAAACACCGGGTGCCGTGTCCAAACAACCAATGATATTCATCAGTGTGGGtttacc                                                                                                                                                                                   >  1:442413/1‑204 (MQ=255)                                                                                 aTTGCAGGTAAGGCGACGTTTTCTGCGGCGGTTAAACTGCTTTATAGGTTATAGCGTTGGGAAATAAAACCGAATTTTTGGCTGCGTAAATCGGAAAACTGATCGTTGGTTAATTCAATGGGTTCTTTTCCATCAATTTTTTATGAACCACCGGTTGCCGTGTCCAAACAACCAATGATATTCATCAGTGTGGGTTTTCCTGAACCAGATTGTCCCATAAATGCGACAAAAACACCTCTTTCGATGGAAAg                                                                                                                                >  1:214682/1‑251 (MQ=255)                                                                                     cAGGTAAGGCGACGTTTTCTGCGGCGGTTAAACTGCTTAATAGGGTATAGCGTTGGAAAATAAAACCGAATTTTTGGCTGCGTAAATCGGAAAGCTGATCGTTGGTTAATTCAATGGGTTTTTTTTCATCAATTTTGTATGAACCACCGGGTGCCGTGTCCAAACAACCAATTATATTCATCAGTGTGGATTTGCCTGAACCAGATTGTCCCATAATGGCGACAAAATCACCTCTTTCGATGGAAAGGGaa                                                                                                                            >  1:10517/1‑251 (MQ=255)                                                                                        gTAAGGCGACGTTTTCTGCGGCGGTTAAGCTGCTTAATAGGTTATAGCGTTGGAAAATAAAACCGAATTTTTGGCTGCGTAAATCGGAAAGCTGATCGTTGGTTAATTCAATGGTTTCTTTTCCATCAATTTTGTATGAACCACCGGTTGCCGTGTCCAAACAACCAATGATATTCATCAGTGTGGATTTGCCTGAACCAGATTGTCCCATAATGGCGACAAAATCACCTCTTTCGATGGAAAGGGaaa                                                                                                                           <  1:627041/249‑1 (MQ=255)                                                                                                            gcggTTAAGCTGCTTAATAGGTTATAGCGTTGGAAAATAAAACCGAATTTTTGGCTGCGTAAATCGGAAAGCTGATCGTTGGTTAATTCAATGGTTTCTTTTCCATCAATTTTGTATGAACCACCGGTTGCCGTGTCCAAACAACCAATGATATTCATCAGTGTGGATTTGCCTGAAcc                                                                                                                                                                             >  1:610811/1‑179 (MQ=255)                                                                                                              ggTTAAGCTGCTTAATAGGTTATAGCGTTGGAAAATAAAACCGAATTTTTGGCTGCGTAAATCGGAAAGCTGATCGTTGGTTAATTCAATGGTTTCTTTTCCATCAATTTTGTATGAACCACCGGTTGCCGTGTCCAAACAACCAATGATATTCATCAGTGTGGATTTGCCTGAACCAGATTGTCCCATAATGGCGACAAAATCACCTCTTTCGATGGAAAGGGAAATg                                                                                                                         <  1:768003/229‑1 (MQ=255)                                                                                                               gTTAAGCTGCTTAATAGGTTATAGCGTTGGAAAATAAAACCGAATTTTTGGCTGCGTAAATCGGAAAGCTGATCGTTGGTTAATTCAATGGTTTCTTTTCCATCAATTTTGTATGAACCACCGGTTGCCGTGTCCAAACAACCAATGATATTCATCAGTGTGGAtt                                                                                                                                                                                       <  1:411892/166‑1 (MQ=255)                                                                                                                       gctTAATAGGTTATAGCGTTGGAAAATAAAACCGAATTTTTGGCTGCGTAAATCGGAAAGCTGATCGTTGGTTAATTCAATGGTTTCTTTTCCATCAATTTTGTATGAacc                                                                                                                                                                                                                                      <  1:472070/111‑1 (MQ=255)                                                                                                                       gctTAATAGGTTATAGCGTTGGAAAATAAAACCGAATTTTTGGCTGCGTAAATCGGAAAGCTGATCGTTGGTTAATTCAATGGTTTCTTTTCCATCAATTTTGTATGAACCACCGGTTGCCGTGTCCAAACAACCAATGATATTCATCAg                                                                                                                                                                                               <  1:822333/150‑1 (MQ=255)                                                                                                                       gctTAATAGGTTATAGCGTTGGAAAATAAAACCGAATTTTTGGCTGCGTAAATCGGAAAGCTGATCGTTGGTTAATTCAATGGTTTCTTTTCCATCAATTTTGTATGAACCACCGGTTGCCGTGTCCAAACAACCAATGATATTCATCAGTGTGGATTTGCCTGAACCAGATTGTCCCATAATGGCGACAAAATCACCTCTTTCGATGGAAAGGGAAATGTCTTTTAAAACATGAACGCgg                                                                                                    <  1:539112/241‑1 (MQ=255)                                                                                                                       gctTAATAGGTTATAGCGTTGGAAAATAAAACCGAATTTTTGGCTGCGTAAATCGGAAAGCTGATCGTTGGTTAATTCAATGGTTTCTTTTCCATCAATTTTGTATGAACCACCGGTTGCCGTGTCCAAACAACCAATGATATTCATCAGTGTGGATTTGCCTGAACCAGATTGTCCCATAATGGCGACAAAATCACCTCTTTCGATGGAAAGGGAAATGTCTTTTAAAACATGAACGCGGTTTTCAc                                                                                             >  1:470704/1‑248 (MQ=255)                                                                                                                       gctTAATAGGTTATAGCGTTGGAAAATAAAACCGAATTTTTGGCTGCGTAAATCGGAAAGCTGATCGTTGCTTAATTCAATGGTTTCTTTTCAATCAATTTTGTATGAACCACCGGTTGCAGTGTCCAAACAACCAATGATATTCATCAGTGTGGATTTGCCTGAACCAGATTGTCCCATAATGGCGACAAAATCACCTCTTTCGATGGAAAGGGAAATGTCTTTTAAAACATGAACGCGGTTTTCACCtt                                                                                          >  1:544412/1‑251 (MQ=255)                                                                                                                               ggTTATAGCGTTGGAAAATAAAACCGAATTTTTGGCTGCGTAAATCGGAAAGCTGATCGTTGGTTAATTCAATGGTTTCTTTTCCATCAATTTTGTATGAACCACCGGTTGCCGTGTCCAAACAACCAATGATATTCATCAGTGTGGATTTGCCTGAACCAGATTGTCCCATAATGGCGACAAAATCACCTCTTTCGATGGAAAGGGaaa                                                                                                                           >  1:798002/1‑210 (MQ=255)                                                                                                                               ggTTATAGCGTTGGAAAATAAAACCGAATTTTTGGCTGCGTAAATCGGAAAGCTGATCGTTGGTTAATTCAATGGTTTCTTTTCCATCAATTTTGTATGAACCACCGGTTGCCGTGTCCAAACAACCAATGATATTCATCAGTGTGGATTTGCCTGAACCAGATTGTCCCATAATGGCGACAAAATCACCTCTTTCGATGGAAAGGGAAATGTCTTTTAAAACATGAACGCGGTTTTCACCTTCGCCaaaa                                                                                  >  1:401270/1‑251 (MQ=255)                                                                                                                                gTTATAGCGTTGGAAAATAAAACCGAATTTTTGGCTGCGTAAATCGGAAAGCTGATCGTTGGTTAATTCAATGGTTTCTTTTCCATCAATTTTGTATGAACCACCGGTTGCCGTGTCCAAACAACCAATGATATtcatc                                                                                                                                                                                                 <  1:281324/139‑1 (MQ=255)                                                                                                                                gTTATAGCGTTGGAAAATAAAACCGAATTTTTGGCTGCGTAAATCGGAAAGCTGATCGTTGGTTAATTCAATGGTTTCTTTTCCATCAATTTTGTATGAACCACCGGTTGCCGTGTCCAAACAACCAATGATATTCATCAGTGTGGATTTGCCTGAACCAGATTGTCCCATAATGGCGACAAAATCACCTCTTTCGATGGAAAGGGAAATGTATTTTAAAACATGAACGCGGTTTTCACCTTCGCCAAAAt                                                                                 >  1:885007/1‑251 (MQ=255)                                                                                                                                 ttATAGCGTTGGAAAATAAAACCGAATTTTTGGCTGCGTAAATCGGAAAGCTGATCGTTGGTTAATTCAATGGTTTCTTTTCCATCAATTTTGTATGAACCACCGGTTGCCGTGTCCAAACAACCAATGATATTCATCAGTGTGGATTTGCCTGAACCAGATTGTCCCATAATGGCGACAAAATCACCTCTTTCGATGGAAAGGGAAATGTCTTTTAAAACATGAACGCGGTTTTCACCTTCGCCAAAAt                                                                                 <  1:74459/250‑1 (MQ=255)                                                                                                                                 ttATAGCGTTGGAAAATAAAACCGAATTTTTGGCTGCGTAAATCGGAAAGCTGATCGTTGGTTAATTCAATGGTTTCTTTTCCATCAATTTTGTATGAACCACCGGTTGCCGTGTCCAAACAACCAATGATATTCATCAGTGTGGATTTGCCTGAACCAGATTGTCCCATAATGGCGACAAAATCACCTCTTTCGATGGAAAGGGAAATGTCTTTTAAAACATGAACGCGGTTTTCACCTTCGCCAAAATa                                                                                >  1:517299/1‑251 (MQ=255)                                                                                                                                      gCGTTGGAAAATAAAACCGAATTTTTGGCTGCGTAAATCGGAAAGCTGATCGTTGGTTAATTCAATGGTTTCTTTTCCATCAATTTTGTATGAACCACCGGTTGCCGTGTCCAAACAACCAATGATATTCATCAGTGTGGATTTGCCTGAACCAGATTGTCCCATAATGGCGACAAAATCACCTCTTTCGATGGAAAGGGAAATGTCTTTTAAAACATGAACGCGGTTTTCACCTTCGcc                                                                                      >  1:74379/1‑240 (MQ=255)                                                                                                                                       cGTTGGAAAATAAAACCGAATTTTTGGCTGCGTAAATCGGAAAGCTGATCGTTGGTTAATTCAATGGTTTCTTTTCCATCAATTTTGTATGAACCACCGGTTGCCGTGTCCAAACAACCAATGATATTCATCAGTGTGGATTTGCCTGAACCAGATTGTCCCATAATGGCGACAAAATCACCTCTTTCGATGGAAAGGGAAATGTCTTTTAAAACATGAACGCGGTTTTCACCTTCGCCAAAATAACGGt                                                                           <  1:627363/250‑1 (MQ=255)                                                                                                                                         ttGGAAAATAAAACCGAATTTTTGGCTGCGTAAATCGGAAAGCTGATCGTTGGTTAATTCAATGGTTTCTTTTCCATCAATTTTGTATGAACCACCGGTTGCCGTGTCCAAACAACCAATGATATTCATCAGTGTGGATTTGCCTGAACCAGATTGTCCCATAATGGCGACAAAATCACCTCTTTCGATGGAAAGGGAAATGTCTTTTAAAACATGAACGCGGTTTTCACCTTCGCCAAAATAACGGTTg                                                                         <  1:854192/250‑1 (MQ=255)                                                                                                                                           ggAAAATAAAACCGAATTTTTGGCTGCGTAAATCGGAAAGCTGATCGTTGGTTAATTCAATGGTTTCTTTTCCATCAATTTTGTATGAACCACCGGTTGCCGTGTCCAAACAACCAATGATATTCATCAGTGTGGATTTGCCTGAACCAGATTGTCCCATAATGGCGACAAAATCACCTCTTTCGATGGAAAGGGAAATGTCTTTTAAAACATGAACGCGGTTTTCACCTTCGCCAAAATAACGGTTGAg                                                                       >  1:460926/1‑250 (MQ=255)                                                                                                                                                              ttGGCTGCGTAAATCGGAAAGCTGATCGTTGGTTAATTCAATGGTTTCTTTTCCATCAATTTTGTATGAACCACCGGTTGCCGTGTCCAAACAACCAATGATATTCATCAGTGTGGATTTGCCTGAACCAGATTGTCCCATAATGGCGACAAAATCACCTCTTTCGATGGAAAGGGAAATGTCTTTTAAAACATGAACGCGGTTTTCACCTTCGCCAAAATAACGGTTGAGCTGCTTGATTTCAATTatat                                                   <  1:798098/251‑1 (MQ=255)                                                                                                                                                              ttGGCTGCGTAAATCGGAAAGCTGATCGTTGGTTAATTCAATGGTTTCTTTTCCATCAATTTTGTATGAACCACCGGTTGCCGTGTCCAAACAACCAATGATATTCATCAGTGTGGATTTGCCTGAACCAGATTGTCCCATAATGGCGACAAAATCACCTCTTTCGATGGAAAGGGAAATGTCTTTTAAAACATGAACGCGGTTTTCACCTTCGCCAAAATAACGGTTGAGCTGCTTGATTTCAATTatat                                                   <  1:678550/251‑1 (MQ=255)                                                                                                                                                                           tCGGAAAGCTGATCGTTGGTTAATTCAATGGTTTCTTTTCCATCAATTTTGTATGAACCACCGGTTGCCGTGTCCAAACAACCAATGATATTCATCAGTGTGGATTTGCCTGAACCAGATTGTCCCATAAt                                                                                                                                                              <  1:888810/131‑1 (MQ=255)                                                                                                                                                                           tCGGAAAGCTGATCGTTGGTTAATTCAATGGTTTCTTTTCCATCAATTTTGTATGAACCACCGGTTGCCGTGTCCAAACAACCAATGATATTCATCAGTGTGGATTTGCCTGAACCAGATTGTCCCATAATGGCGACAAAATCACCTCTTTCGATGGAAAGGGAAATGTCTTTTAAAACATGAACGCGGTTTTCACCTTCGCCAAAATAACGGTTGAGCTGCTTGATTTCAATTATATTCATCGTCGaaa                                       >  1:352755/1‑250 (MQ=255)                                                                                                                                                                              gAAAGCTGATCGTTGGTTAATTCAATGGTTTCTTTTCCATCAATTTTGTATGAACCACCGGTTGCCGTATCCAAACAACCAATGATATTCATCAGTGTGGATTTGCCTGAACCAGATTGTCCCATAATGGCGACAAAATCACCTCTTTCGATGGAAAGGGAAATGTCTTTTAAAACATGAACGCGGTTTTCACCTTCGCCAAAATAACGGTTGAGCTGCTTGATTTCAATTATATTCATCGTCGAAAccc                                    <  1:28133/250‑1 (MQ=255)                                                                                                                                                                                  gCTGATCGTTGGTTAATTCAATGGTTTCTTTTCCATCAATTTTGTATGAACCACCGGTTGCCGTGTCCAAACAACCAATGATATTCATCAGTGTGGATTTGCCTGAACCAGATTGTCCCATAATGGCGACAAAATCACCTCTTTCGATGGAAAGGGAAATGTCTTTTAAAACATGAACGCGGTTTTCACCTTCGCCAAAATAACGGTTGAGCTGCTTGATTTCAATTatat                                                   >  1:283954/1‑231 (MQ=255)                                                                                                                                                                                  gCTGATCGTTGGTTAATTCAATGGTTTCTTTTCCATCAATTTTGTATGAACCACCGGTTGCCGTGTCCAAACAACCAATGATATTCATCAGTGTGGATTTGCCTGAACCAGATTGTCCCATAATGGCGACAAAATCACCTCTTTCGATGGAAAGGGAAATGTCTTTTAAAACATGAACGCGGTTTTCACCTTCGCCAAAATAACGGTTGAGCTGCTTGATTTCAATTATATTCATCGTCGAAACCCACCg                                >  1:564590/1‑250 (MQ=255)                                                                                                                                                                                            ggTTAATTCAATGGTTTCTTTTCCATCAATTTTGTATGAACCACCGGTTGCCGTGTCCAAACAACCAATGATATTCATCAGTGTGGATTTGCCTGAACCAGATTGTCCCATAATGGCGACAAAATCACCTCTTTCGATGGAAAGGGAAATGTCTTTTAAAACATGAACGCGGTTTTCACCTTCGCCAAAATAACGGTTGAGCTGCTTGATTTCAATTATATTCATCGTCGAAACCCACCGCACTTTagaag                     >  1:621601/1‑251 (MQ=255)                                                                                                                                                                                             gTTAATTCAATGGTTTCTTTTCCATCAATTTTGTATGAACCACCGGTTGCCGTGTCCAAACAACCAATGATATTCATCAGTGTGGATTTGCCTGAACCAGATTGTCCCATAATGGCGACAAAATCACCTCTTTCGATGGAAAGGGAAATGTCTTTTAAAACATGAACGCGGTTTTCACCTTCGCCAAAATAACGGTTGAGCTGCTTGATTTCAATTATATTCATCGTCGAAACCCACCGCACTTTagaag                     <  1:741209/250‑1 (MQ=255)                                                                                                                                                                                             gTTAATTCAATGGTTTCTTTTCCATCAATTTTGTATGAACCACCGGTTGCCGTGTCCAAACAACCAATGATATTCATCAGTGTGGATTTGCCTGAACCAGATTGTCCCATAATGGCGACAAAATCACCTCTTTCGATGGAAAGGGAAATGTCTTTTAAAACATGAACGCGGTTTTCACCTTCGCCAAAATAACGGTTGAGCTGCTTGATTTCAATTATATTCATCGTCGAAACCCACCGCACTTTagaag                     <  1:621896/250‑1 (MQ=255)                                                                                                                                                                                                 aTTCAATGGTTTCTTTTCCATCAATTTTGTATGAACCACCGGTTGCCGTGTCCAAACAACCAATGATATTCATCAGTGTGGATTTGCCTGAACCAGATTGTCCCATAATGGCGACAAAATCACCTCTTTCGATGGAAAGGGAAATGTCTTTTAAAACATGAACGCGGTTTTCACCTTCGCCAAAATAACGGTTGAGCTGCTTGATTTCAATTATATTc                                                 >  1:110141/1‑218 (MQ=255)                                                                                                                                                                                                 aTTCAATGGTTTCTTTTCCATCAATTTTGTATGAACCACCGGTTGCCGTGTCCAAACAACCAATGATATTCATCAGTGTGGATTTGCCTGAACCAGATTGTCCCATAATGGCGACAAAATCACCTCTTTCGATGGAAAGGGAAATGTCTTTTAAAACATGAACGCGGTTTTCACCTTCGCCAAAATAACGGTTGAGCTGCTTGATTTCAATTATATTCATCGTCGAAACCCACCGCACTTTAGAAGATAAt                >  1:811760/1‑251 (MQ=255)                                                                                                                                                                                                    cAATGGTTTCTTTTCCATCAATTTTGTATGAACCACCGGTTGCCGTGTCCAAACAACCAATGATATTCATCAGTGTGGATTCGCCTGAACCAGATTGTCCCATAATGGCGACAAAATCACCTCTTTCGATGGAAAGGGAAATGTCTTTTAAAACATGAACGCGGTTTTCACCTTCGCCAAAATAACGGTTGAGCTGCTTGATTTCAATTATATTCATCGTCGAAACCCACCGCACTTTAGAAGATAATCgg             <  1:431996/251‑1 (MQ=255)                                                                                                                                                                                                             cTTTTCCATCAATTTTGTATGAACCACCGGTTGCCGTGTCCAAACAACCAATGATATTCATCAGTGTGGATTTGCCTGAACCAGATTGTCCCATAATGGCGACAAAATCACCTCTTTCGATGGAAAGGGAAATGTCTTTTAAAACATGAACGCGGTTTTCACCTTCGCCAAAATAACGGTTGAGCTGCTTGATTTCAATTATATTCATCGTCGAAACCCACCGCACTTTAGAAGATAATCGGGGCATCAgg    <  1:603675/251‑1 (MQ=255)                                                                                                                                                                                                              ttttCCATCAATTTTGTATGAACCACCGGTTGCCGTGTCCAAACAACCAATGATATTCATCAGTGTGGATTTGCCTGAACCAGATTGTCCCATAATGGCGACAAAATCACCTCTTTCGATGGAAAGGGAAATGTCTTTTAAAACATGAACGCGGTTTTCACCTTCGCCAAAATAACGGTTGAGCTGCTTGATTTCAATTATATTCATCGTCGAAACCCACCGCACTTTAGAAGATAATCGGGGCATCAGGa   <  1:166774/251‑1 (MQ=255)                                                                                                                                                                                                               tttCCATCAATTTTGTATGAACCACCGGTTGCCGTGTCCAAACAACCAATGATATTCATCAGTGTGGATTTGCCTGAACCAGATTGTCCCATAATGGCGACAAAATCACCTCTTTCGATGGAAAGGGAAATGTCTTTTAAAACATGAACGCGGTTTTCACCTTCGCCAAAATAACGGTTGAGCTGCTTGATTTCAATTATATTCATCGTCGAAACCCACCGCACTTTAGAAGATAATCGGGGCATCAGGAt  <  1:50618/251‑1 (MQ=255)                                                                                                                                                                                                                   cATCAATTTTGTATGAACCACCGGTTGCCGTGTCCAAACAACCAATGATATTCATCAGTGTGGATTTGCCTGAACCAGATTGTCCCATAATGGCGACAAAATCACCTCTTTCGATGGAAAGGGAAATGTCTTTTAAAACATGAACGCGGTTTTCACCTTCGCCAAAATAACGGTTGAGCTGCTTGATTTCAATTATATTCATCGTCGAAACCCACCGCACTTTagaag                     <  1:548288/228‑1 (MQ=255)                                                                                                                                                                                                                        aTTTTGTATGAACCACCGGTTGCCGTGTCCAAACAACCAATGATATTCATCAGTGTGGATTTGCCTGAACCAGATTGTCCCATAATGGCGACAAAATCACCTCTTTCGATGGAAAGGGAAATGTCTTTTAAAACATGAACGCGGTTTTCACCTTCGCCAAAATAACGGTTGAGCTGCTTGATTTCAATTATATTc                                                 >  1:65191/1‑195 (MQ=255)                                                                                                                                                                                                                              |                                                                                                                                                                                                                                            GCCATTTATCGCCCAATCCCAGTTTTTCCAATAATTGTTTGGCACGTTCCAAGCGTTGGCTTTGTGGCATTCCCGCATAAATTGCAGGTAAGGCGACGTTTTCTGCGGCGGTTAAGCTGCTTAATAGGTTATAGCGTTGGAAAATAAAACCGAATTTTTGGCTGCGTAAATCGGAAAGCTGATCGTTGGTTAATTCAATGGTTTCTTTTCCATCAATTTTGGATGAACCACCGGTTGCCGTGTCCAAACAACCAATGATATTCATCAGTGTGGATTTGCCTGAACCAGATTGTCCCATAATGGCGACAAAATCACCTCTTTCGATGGAAAGGGAAATGTCTTTTAAAACATGAACGCGGTTTTCACCTTCGCCAAAATAACGGTTGAGCTGCTTGATTTCAATTATATTCATCGTCGAAACCCACCGCACTTTAGAAGATAATCGGGGCATCAGGAT  >  1/1260844‑1261300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑