Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,255,510	A→C	intergenic (+116380/+137801)	unknown → / ← unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,255,510	0	A	C	100.0%	169.3 / NA	66	intergenic (+116380/+137801)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (47/19);  total (47/19)

ATTAGAAAACTGGGAAAGACGACTTATAAAAGGAAGAAAAGGGAAAACATACGAATACCATTATTTATCCATGCCTTACGAGGTGCAGCGCGCCCTAGGCTTTAAACGCAATATAATAGAAGAGCCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCAGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAGCGCTAACGAGAAGATTATTAATTTAGTTAAATTGGTTGCATAAACTAAGGAGAGTTATGGACAAGCAGTCAATTCTTAAATTCTTTAATGATAATACTAAGGACGCGCCACAAGGCAGCATATACATTCAATATGGCAACAACTATACAACAAATGACATCTGTATAGCAG  >  1/1255267‑1255734                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                 aTTAGAAAACTGGGAAAGACGACTTATAAAAGGAAGAAAAGGGAAAACATACGAATACCATTATTTATCCATGCCTTACGAGGTGCAGCGCGCCCTAGGCTTTAAACGCAATATAATAGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCa                                                                                                                                                                                                                           >  1:830752/1‑251 (MQ=255)  ttAGAAAACTGGGAAAGACGACTTATAAAAGGAAGAAAAGGGAAAACATACGAATACCATTATTTATCCATGCCTTACGAGGTGCAGCGCGCCCTAGGCTTTAAACGCAATATAATAGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCaa                                                                                                                                                                                                                          >  1:319097/1‑251 (MQ=255)        aaCTGGGAAAGACGACTTATAAAAGGAAGAAAAGGGAAAACATACGAATACCATTATTTATCCATGCCTTACGAGGTGCAGCGCGCCCTAGGCTTTAAACGCAATATAATAGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCa                                                                                                                                                                                                                    >  1:464/1‑251 (MQ=255)                                     aaaaGGGAAAACATACGAATACCATTATTTATCCATGCCTTACGAGGTGCAGCGCGCCCTAGGCTTTAAACGCAATATAATAGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTa                                                                                                                                                                                        <  1:189513/250‑1 (MQ=255)                                           gAAAACATACGAATACCATTATTTATCCATGCCTTACGAGGTGCAGCGCGCCCTAGGCTTTAAACGCAATATAATAGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGaa                                                                                                                                                                                              >  1:729569/1‑238 (MQ=255)                                             aaaCATACGAATACCATTATTTATCCATGCCTTACGAGGTGCAGCGCGCCCTAGGCTTTAAACGCAATATAATAGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCgaagaa                                                                                                                                                                               >  1:112038/1‑251 (MQ=255)                                               aCATACGAATACCATTATTTATCCATGCCTTACGAGGTGCAGCGCGCCCTAGGCTTTAAACGCAATATAATAGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAg                                                                                                                                                                             >  1:47706/1‑251 (MQ=255)                                                          ccATTATTTATCCATGCCTTACGAGGTGCAGCGCGCCCTAGGCTTTAAACGCAATATAATAGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGaa                                                                                                                                                                                              >  1:823092/1‑223 (MQ=255)                                                             ttatttatCCATGCCTTACGAGGTGCAGCGCGCCCTAGGCTTTAAACGCAATATAATAGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAGCGCTAACGagaaga                                                                                                                                                               >  1:332880/1‑251 (MQ=255)                                                             ttatttatCCATGCCTTACGAGGTGCAGCGCGCCCTAGGCTTTAAACGCAATATAATAGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAGCGCTAACGagaag                                                                                                                                                                <  1:470275/250‑1 (MQ=255)                                                              tatttatCCATGCCTTACGAGGTGCAGCGCGCCCTAGGCTTTAAACGCAATATAATAGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAGCGCTAACGAGAAGat                                                                                                                                                              >  1:694608/1‑251 (MQ=255)                                                                         gCCTTACGAGGTGCAGCGCGCCCTAGGCTTTAAACGCAATATAATAGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAGCGCTAACGAGAAGATTATTAATTTAg                                                                                                                                                   >  1:572260/1‑251 (MQ=255)                                                                                   gTGCAGCGCGCCCTAGGCTTTAAACGCAATATAATAGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGaa                                                                                                                                                                                              >  1:493123/1‑198 (MQ=255)                                                                                   gTGCAGCGCGCCCTAGGCTTTAAACGCAATATAATAGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAGCGCTAACGAGAAGATTATTAAt                                                                                                                                                       >  1:878318/1‑237 (MQ=255)                                                                                            gcCCTAGGCTTTAAACGCAATATAATAGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAGCGCTAACGAGAAGATTATTAATTTAGTTAAATTGGTTGCATaaa                                                                                                                                 >  1:415189/1‑250 (MQ=255)                                                                                             cccTAGGCTTTAAACGCAATATAATAGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACTCGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGgatgat                                                                                                                                                                                                     <  1:767249/181‑1 (MQ=255)                                                                                             cccTAGGCTTTAAACGCAATATAATAGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAGCGCTAACGAGAAGATTATTAATTTAGTTAAATTGGTTGCATaaa                                                                                                                                 >  1:723616/1‑249 (MQ=255)                                                                                                    cTTTAAACGCAATATAATAGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAGCGCTAACGAGAAGATTATTAATTTAGTTAAATTGGTTGCATAAACTAAGgag                                                                                                                         <  1:852450/250‑1 (MQ=255)                                                                                                          aCGCAATATAATAGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGcc                                                                                                                                                                                                                            >  1:286589/1‑145 (MQ=255)                                                                                                             cAATATAATAGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAGCGCTAACGAGAAGATTATTAATTTAGTTAAATTGGTTGCATAAACTAAGGAGAGTTATGGa                                                                                                                >  1:854002/1‑250 (MQ=255)                                                                                                               atatAATAGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATgg                                                                                                                                                                                                               >  1:285794/1‑153 (MQ=255)                                                                                                               atatAATAGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAGCGCTAACGAGAAGATTATTAATTTAGTTAAATTGGTTGCATAAACTAAGGAGAGTTATGGAc                                                                                                               >  1:876946/1‑249 (MQ=255)                                                                                                                 ataataGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAGCGCTAACGAGAAGATTATTAATTTAGTTAAATTGGTTGCATAAACTAAGGAGAGTTATGGACAAg                                                                                                            >  1:253706/1‑250 (MQ=255)                                                                                                                  taataGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGaaa                                                                                                                                                                              >  1:833169/1‑183 (MQ=255)                                                                                                                  taataGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAGCGCTAACGAGAAGATTATTAATTTAGTTAAATTGGTTGCATAAACTAAGGAGAGTTATGGACAAGCa                                                                                                          >  1:752460/1‑251 (MQ=255)                                                                                                                  taataGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAGCGCTAACGAGAAGATTATTAATTTAGTTAAATTGGTTGCATAAACTAAGGAGAGTTATGGACAAGCa                                                                                                          >  1:218147/1‑251 (MQ=255)                                                                                                                  taataGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAGCGCTAACGAGAAGATTATTAATTTAGTTAAATTGGTTGCATAAACTAAGGAGAGTTATGGACAAGCa                                                                                                          >  1:23326/1‑251 (MQ=255)                                                                                                                        aagaGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAGCGCTAACGAGAAGATTATTAATTTAGTTAAATTGGTTGCATAAACTAAGGAGAGTTATGGACAAGCAGTCAAt                                                                                                    <  1:185669/251‑1 (MQ=255)                                                                                                                            gtcTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAGCGCTAACGAGAAGATTATTAATTTAGTTAAATTGGTTGCATAAACTAAGGAGAGTTATgg                                                                                                                 >  1:727140/3‑234 (MQ=255)                                                                                                                              cTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGgatg                                                                                                                                                                                                       >  1:365010/1‑146 (MQ=255)                                                                                                                                 ccGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAGCGCTAACGAGAAGATTATTAATTTAGTTAAATTGGTTGCATAAACTAAgg                                                                                                                           >  1:735222/1‑219 (MQ=255)                                                                                                                                   gCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAGCGCTAACGAGAAGATTATTAATTTAGTTAAATTGGTTGCATAAACTAAGGAGAGTTATGGACAAGCAGTCAATTCTTAAAtt                                                                                           >  1:874746/1‑249 (MQ=255)                                                                                                                                   gCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAGCGCTAACGAGAAGATTATTAATTTAGTTAAATTGGTTGCATAAACTAAGGAGAGTTATGGACAAGCAGTCAATTCTTAAAtt                                                                                           >  1:302979/1‑249 (MQ=255)                                                                                                                                      caATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAGCGCTAACGAGAAGATTATTAATTTAGtt                                                                                                                                                 <  1:230423/192‑1 (MQ=255)                                                                                                                                        atatAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGaa                                                                                                                                                                                              <  1:463967/145‑1 (MQ=255)                                                                                                                                        atatAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAGCGCTAACGAGAAGATTATTAATTTAGTTAAATTGGTTGCATAAACTAAGgag                                                                                                                         >  1:72388/1‑214 (MQ=255)                                                                                                                                        atatAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAGCGCTAACGAGAAGATTATTAATTTAGTTAAATTGGTTGCATAAACTAAGGAGAGTTATgg                                                                                                                 <  1:708853/222‑1 (MQ=255)                                                                                                                                        atatAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAGCGCTAACGAGAAGATTATTAATTTAGTTAAATTGGTTGCATAAACTAAGGAGAGTTATGGACAAGCAGTCAATTCt                                                                                                 <  1:404192/238‑1 (MQ=255)                                                                                                                                        atatAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAGCGCTAACGAGAAGATTATTAATTTAGTTAAATTGGTTGCATAAACTAAGGAGAGTTATGGACAAGCAGTCAATTCTTAAATTCTTTAAt                                                                                    >  1:634670/1‑251 (MQ=255)                                                                                                                                        atatAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAGCGCTAACGAGAAGATTATTAATTTAGTTAAATTGGTTGCATAAACTAAGGAGAGTTATGGACAAGCAGTCAATTCTTAAATTCTTTAAt                                                                                    >  1:111645/1‑251 (MQ=255)                                                                                                                                          ataACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAGCGCTAACGAGAAGATTATTAATTTAGTTAAATTGGTTGCATAAACTAAGGAGAGTTATGGACAAGCAGTCAATTCTTAAATTCTTTAATGa                                                                                  <  1:570980/251‑1 (MQ=255)                                                                                                                                                cATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGaa                                                                                                                                                                                              >  1:79157/1‑137 (MQ=255)                                                                                                                                                     aaaGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAGCGCTAACGagaag                                                                                                                                                                >  1:423023/1‑162 (MQ=37)                                                                                                                                                     aaaGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAGCGCTAACGAGAAGATTATTAATTTAGTTAAATTGGTTGCATAAACTAAgg                                                                                                                           >  1:287294/1‑199 (MQ=25)                                                                                                                                                                    gAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAGCGCTAACGAGAAGATTATTaa                                                                                                                                                        <  1:321064/155‑1 (MQ=17)                                                                                                                                                                      aGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAGCGCTAACGAGAAGATTATTAATTTAGTTAAATTGGTTGCATAAACTAAGgag                                                                                                                         >  1:81330/1‑184 (MQ=17)                                                                                                                                                                      aGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAGCGCTAACGAGAAGATTATTAATTTAGTTAAATTGGTTGCATAAACTAAGGAGAGTTATGGACAAGCAGTCAATTCTTAAATTCTTTAATGATAATACTAAGGACGCGCCACAAGgcag                                                       >  1:6797/1‑250 (MQ=14)                                                                                                                                                                      aGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAGCGCTAACGAGAAGATTATTAATTTAGTTAAATTGGTTGCATAAACTAAGGAGAGTTATGGACAAGCAGTCAATTCTTAAATTCTTTAATGATAATACTAAGGACGCGCCACAAGgcag                                                       >  1:154283/1‑250 (MQ=14)                                                                                                                                                                        tAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAGCGCTAACGAGAAGATTATTAATTTAGTTAAATTGGTTGCATAAACTAAGGAGAGTTATGGACAAGCAGTCAATTCTTAAATTCTTTAATGATAATACTAAGGACGCGCCACAAGGCAGCat                                                    >  1:745630/1‑251 (MQ=14)                                                                                                                                                                            cAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAGCGCTAACGAGAAGATTATTAATTTAGTTAAATTGGTTGCATAAACTAAGGAGAGTTATGGACAAGCAGTCAATTCTTAAATTCTTTAATGATAATACTAAGGACGCGCCACAAGGCAGCATATAc                                                <  1:849176/251‑1 (MQ=14)                                                                                                                                                                                   cgcTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAGCGCTAACGAGAAGATTATTAATTTAGTTAAATTGGTTGCATAAACTAAGGAGAGTTATGGACAAGCAGTCAATTCTTAAATTCTTTAATGATAATACTAAGGACGCGCCACAAGGCAGCATATACATTCaa                                          <  1:805082/250‑1 (MQ=14)                                                                                                                                                                                     cTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGaa                                                                                                                                                                                              <  1:296660/100‑1 (MQ=21)                                                                                                                                                                                     cTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAGCGCTAACGAGAAGATTATTAATTTAGTTAAATTGGTTGCATAAACTAAGGAGAGTTATGGACAAGCAGTCAATTCTTAAATTCTTTAATGATAATACTAAGGACGCGCCACAAGGCAGCATATACAt                                              >  1:343897/1‑244 (MQ=14)                                                                                                                                                                                     cTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAGCGCTAACGAGAAGATTATTAATTTAGTTAAATTGGTTGCATAAACTAAGGAGAGTTATGGACAAGCAGTCAATTCTTAAATTCTTTAATGATAATACTAAGGACGCGCCACAAGGCAGCATATACATTCAatat                                       >  1:125072/1‑251 (MQ=14)                                                                                                                                                                                     cTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAGCGCTAACGAGAAGATTATTAATTTAGTTAAATTGGTTGCATAAACTAAGGAGAGTTATGGACAAGCAGTCAATTCTTAAATTCTTTAATGATAATACTAAGGACGCGCCACAAGGCAGCATATACATTCAatat                                       >  1:256609/1‑251 (MQ=14)                                                                                                                                                                                       ttttAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTa                                                                                                                                                                                                             >  1:309070/1‑83 (MQ=25)                                                                                                                                                                                       ttttAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCgaagaa                                                                                                                                                                               >  1:279847/1‑113 (MQ=18)                                                                                                                                                                                       ttttAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAGCGCTAACGAGAAGATTATTaa                                                                                                                                                        <  1:289276/136‑1 (MQ=18)                                                                                                                                                                                       ttttAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAGCGCTAACGAGAAGATTATTAATTTAGTTAAATTg                                                                                                                                           >  1:278736/1‑149 (MQ=17)                                                                                                                                                                                       ttttAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAGCGCTAACGAGAAGATTATTAATTTAGTTAAATTGGTTGCATAAACTAAGGAGAGTTATGGACAAGCAGTCAATTCTTAAATTCTTTAATGATAATACTAAGGACGCGCCACAAGGCAGCATATACa                                               >  1:128528/1‑241 (MQ=14)                                                                                                                                                                                       ttttAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAGCGCTAACGAGAAGATTATTAATTTAGTTAAATTGGTTGCATAAACTAAGGAGAGTTATGGACAAGCAGTCAATTCTTAAATTCTTTAATGATAATACTAAGGACGCGCCACAAGGCAGCATATACATTCAATATgg                                     >  1:776223/1‑251 (MQ=14)                                                                                                                                                                                                       cacaCTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAGCGCTAACGAGAAGATTATTAATTTAGTTAAATTGGTTGCATAAACTAAGGAGAGTTATGGACAAGCAGTCAATTCTTAAATTCTTTAATGATAATACTAAGGACGCGCCACAAGGCAGCATATACATTCAATATGGCAACAACTATacaa                       <  1:482717/249‑1 (MQ=17)                                                                                                                                                                                                         cacTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAGCGCTAACGAGAAGATTATTAATTTAGTTAAATTGGTTGCATAAACTAAGGAGAGTTATGGACAAGCAGTCAATTCTTAAATTCTTTAATGATAATACTAAGGACGCGCCACAAGGCAGCATATACATTCAATATGGCAACAACTATACAACaaa                   >  1:164065/1‑251 (MQ=14)                                                                                                                                                                                                                           cAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAGCGCTAACGAGAAGATTATTAATTTAGTTAAATTGGTTGCATAAACTAAGGAGAGTTATGGACAAGCAGTCAATTCTTAAATTCTTTAATGATAATACTAAGGACGCGCCACAAGGCAGCATATACATTCAATATGGCAACAACTATACAACAAATGACATCTGTATAGCAg  <  1:73859/250‑1 (MQ=11)                                                                                                                                                                                                                           cAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAGCGCTAACGAGAAGATTATTAATTTAGTTAAATTGGTTGCATAAACTAAGGAGAGTTATGGACAAGCAGTCAATTCTTAAATTCTTTAATGATAATACTAAGGACGCGCCACAAGGCAGCATATACATTCAATATGGCAACAACTATACAACAAATGACATCTGTATAGCAg  <  1:845690/250‑1 (MQ=11)                                                                                                                                                                                                                           cAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAGCGCTAACGAGAAGATTATTAATTTAGTTAAATTGGTTGCATAAACTAAGGAGAGTTATGGACAAGCAGTCAATTCTTAAATTCTTTAATGATAATACTAAGGACGCGCCACAAGGCAGCATATACATTCAATATGGCAACAACTATACAACAAATGACATCTGTATAGCAg  <  1:731311/250‑1 (MQ=11)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                 ATTAGAAAACTGGGAAAGACGACTTATAAAAGGAAGAAAAGGGAAAACATACGAATACCATTATTTATCCATGCCTTACGAGGTGCAGCGCGCCCTAGGCTTTAAACGCAATATAATAGAAGAGCCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCAGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAGCGCTAACGAGAAGATTATTAATTTAGTTAAATTGGTTGCATAAACTAAGGAGAGTTATGGACAAGCAGTCAATTCTTAAATTCTTTAATGATAATACTAAGGACGCGCCACAAGGCAGCATATACATTCAATATGGCAACAACTATACAACAAATGACATCTGTATAGCAG  >  1/1255267‑1255734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑