Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,255,391	C→T	intergenic (+116261/+137920)	unknown → / ← unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,255,391	0	C	T	100.0%	157.6 / NA	54	intergenic (+116261/+137920)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (38/16);  total (38/16)

GTTTTAATCCTTGGTTTAAAACTAGGAGGTTAGATATGATCCGAGAGTGGCTAGAACTGAAAGACCTTTTGGGAGTAGCTGGACTACCAGAAACTGTTCAAGGGGTAACTAAAAAAGCCAAATTAGAAAACTGGGAAAGACGACTTATAAAAGGAAGAAAAGGGAAAACATACGAATACCATTATTTATCCATGCCTTACGAGGTGCAGCGCGCCCTAGGCTTTAAACGCAATATAATAGAAGAGCCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCAGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAGCGCTAACGAGAAGATTATTAATTTAGTTAAATTGGTTGCATAAACTAAGGAGAGTTATGGACAAGCAGTCAAT  >  1/1255146‑1255636                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      gTTTTAATCCTTGGTTTAAAACTAGGAGGTTAGATATGATCCGAGAGTGGCTAGAACTGAAAGACCTTTTGGGAGTAGCTGGACTACCAGAAACTGTTCAAGGGGTAACTAAAAAAGCCAAATTAGAAAACTGGGAAAGACGACTTATAAAAGGAAGAAAAGGGAAAACATACGAATACCATTATTTATCCATGCCTTACGAGGTGCAGCGCGCCCTAGGCTTTAAACGCAATATAATAGAAGAGTCTAc                                                                                                                                                                                                                                                   >  1:522773/1‑250 (MQ=255)          cTTGGTTTAAAACTAGGAGGTTAGATATGATCCGAGAGTGGCTAGAACTGAAAGACCTTTTGGGAGTAGCTGGACTACCAGAAACTGTTCAAGGGGTAACTAAAAAAGCCAAATTAGAAAACTGGGAAAGACGACTTATAAAAGGAAGAAAAGGGAAAACATACGAATACCATTATTTATCCATGCCTTACGAGGTGCAGCGCGCCCTAGGCTTTAAACGCAATATAATAGAAGAGTCTACCGCACAatat                                                                                                                                                                                                                                         <  1:646406/251‑1 (MQ=255)               tttAAAACTAGGAGGTTAGATATGATCCGAGAGTGGCTAGAACTGAAAGACCTTTTGGGAGTAGCTGGACTACCAGAAACTGTTCAAGGGGTAACTAAAAAAGCCAAATTAGAAAACTGGGAAAGACGACTTATAAAAGGAAGAAAAGGGAAAACATACGAATACCATTATTTATCCATGCCTTACGAGGTGCAGCGCGCCCTAGGCTTTAAACGCAATATAATAGAAGAGTCTACCGCACAATATAACGc                                                                                                                                                                                                                                    <  1:226745/251‑1 (MQ=255)                      cTAGGAGGTTAGATATGATCCGAGAGTGGCTAGAACTGAAAGACCTTTTGGGAGTAGCTGGACTACCAGAAACTGTTCAAGGGGTAACTAAAAAAGCCAAATTAGAAAACTGGGAAAGACGACTTATAAAAGGAAGAAAAGGGAAAACATACGAATACCATTATTTATCCATGCCTTACGAGGTGCAGCGCGCCCTAGGCTTTAAACGCAATATAATAGAAGAGTCTACCGcac                                                                                                                                                                                                                                              <  1:526286/234‑1 (MQ=255)                            ggTTAGATATGATCCGAGAGTGGCTAGAACTGAAAGACCTTTTGGGAGTAGCTGGACTACCAGAAACTGTTCAAGGGGTAACTAAAAAAGCCAAATTAGAAAACTGGGAAAGACGACTTATAAAAGGAAGAAAAGGGAAAACATACGAATACCATTATTTATCCATGCCTTACGAGGTGCAGCGCGCCCTAGGCTTTAAACGCAATATAATAGAAGAGTCTACCGCACAATATAAc                                                                                                                                                                                                                                      <  1:407825/236‑1 (MQ=255)                               tAGATATGATCCGAGAGTGGCTAGAACTGAAAGACCTTTTGGGAGTAGCTGGACTACCAGAAACTGTTCAAGGGGTAACTAAAAAAGCCAAATTAGAAAACTGGGAAAGACGACTTATAAAAGGAAGAAAAGGGAAAACATACGAATACCATTATTTATCCATGCCTTACGAGGTGCAGCGCGCCCTAGGCTTTAAACGCAATATAATAGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTa                                                                                                                                                                                                                    >  1:403446/1‑251 (MQ=255)                                 gATATGATCCGAGAGTGGCTAGAACTGAAAGACCTTTTGGGAGTAGCTGGACTACCAGAAACTGTTCAAGGGGTAACTAAAAAAGCCAAATTAGAAAACTGGGAAAGACGACTTATAAAAGGAAGAAAAGGGAAAACATACGAATACCATTATTTATCCATGCCTTACGAGGTGCAGCGCGCCCTAGGCTTTAAACGCAATATAATAGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTaa                                                                                                                                                                                                                   >  1:358351/1‑250 (MQ=255)                                 gATATGATCCGAGAGTGGCTAGAACTGAAAGACCTTTTGGGAGTAGCTGGACTACCAGAAACTGTTCAAGGGGTAACTAAAAAAGCCAAATTAGAAAACTGGGAAAGACGACTTATAAAAGGAAGAAAAGGGAAAACATACGAATACCATTATTTATCCATGCCTTACGAGGTGCAGCGCGCCCTAGGCTTTAAACGCAATATAATAGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTaa                                                                                                                                                                                                                   >  1:357377/1‑250 (MQ=255)                                       aTCCGAGAGTGGCTAGAACTGAAAGACCTTTTGGGAGTAGCTGGACTACCAGAAACTGTTCAAGGGGTAACTAAAAAAGCCAAATTAGAAAACTGGGAAAGACGACTTATAAAAGGAAGAAAAGGGAAAACATACGAATACCATTATTTATCCATGCCTTACGAGGTGCAGCGCGCCCTAGGCTTTAAACGCAATATAATAGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAg                                                                                                                                                                                                             <  1:230592/250‑1 (MQ=255)                                            agagTGGCTAGAACTGAAAGACCTTTTGGGAGTAGCTGGACTACCAGAAACTGTTCAAGGGGTAACTAAAAAAGCCAAATTAGAAAACTGGGAAAGACGACTTATAAAAGGAAGAAAAGGGAAAACATACGAATACCATTATTTATCCATGCCTTACGAGGTGCAGCGCGCCCTAGGCTTTAAACGCAATATAATAGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCa                                                                                                                                                                                                       >  1:368594/1‑251 (MQ=255)                                                 ggCTAGAACTGAAAGACCTTTTGGGAGTAGCTGGACTACCAGAAACTGTTCAAGGGGTAACTAAAAAAGCCAAATTAGAAAACTGGGAAAGACGACTTATAAAAGGAAGAAAAGGGAAAACATACGAATACCATTATTTATCCATGCCTTACGAGGTGCAGCGCGCCCTAGGCTTTAAACGCAATATAATAGAAGAGTCTACCGCACAatat                                                                                                                                                                                                                                         <  1:373073/212‑1 (MQ=255)                                                 ggCTAGAACTGAAAGACCTTTTGGGAGTAGCTGGACTACCAGAAACTGTTCAAGGGGTAACTAAAAAAGCCAAATTAGAAAACTGGGAAAGACGACTTATAAAAGGAAGAAAAGGGAAAACATACGAATACCATTATTTATCCATGCCTTACGAGGTGCAGCGCGCCCTAGGCTTTAAACGCAATATAATAGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGgcg                                                                                                                                                                                                  >  1:687382/1‑251 (MQ=255)                                                               gACCTTTTGGGAGTAGCTGGACTACCAGAAACTGTTCAAGGGGTAACTAAAAAAGCCAAATTAGAAAACTGGGAAAGACGACTTATAAAAGGAAGAAAAGGGAAAACATACGAATACCATTATTTATCCATGCCTTACGAGGTGCAGCGCGCCCTAGGCTTTAAACGCAATATAATAGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACgg                                                                                                                                                                                     >  1:70638/1‑250 (MQ=255)                                                                    tttGGGAGTAGCTGGACTACCAGAAACTGTTCAAGGGGTAACTAAAAAAGCCAAATTAGAAAACTGGGAAAGACGACTTATAAAAGGAAGAAAAGGGAAAACATACGAATACCATTATTTATCCATGCCTTACGAGGTGCAGCGCGCCCTAGGCTTTAAACGCAATATAATAGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCa                                                                                                                                                                               >  1:123657/1‑251 (MQ=255)                                                                                tGGACTACCAGAAACTGTTCAAGGGGTAACTAAAAAAGCCAAATTAGAAAACTGGGAAAGACGACTTATAAAAGGAAGAAAAGGGAAAACATACGAATACCATTATTTATCCATGCCTTACGAGGTGCAGCGCGCCCTAGGCTTTAAACGCAATATAATAGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTgaag                                                                                                                                                                   <  1:811320/251‑1 (MQ=255)                                                                                      aCCAGAAACTGTTCAAGGGGTAACTAAAAAAGCCAAATTAGAAAACTGGGAAAGACGACTTATAAAAGGAAGAAAAGGGAAAACATACGAATACCATTATTTATCCATGCCTTACGAGGTGCAGCGCGCCCTAGGCTTTAAACGCAATATAATAGAAGAGTCTACCGCACAATATAAc                                                                                                                                                                                                                                      <  1:246757/178‑1 (MQ=255)                                                                                      aCCAGAAACTGTTCAAGGGGTAACTAAAAAAGCCAAATTAGAAAACTGGGAAAGACGACTTATAAAAGGAAGAAAAGGGAAAACATACGAATACCATTATTTATCCATGCCTTACGAGGTGCAGCGCGCCCTAGGCTTTAAACGCAATATAATAGAAGAGTCTACCGCACAATATAACGc                                                                                                                                                                                                                                    >  1:391216/1‑180 (MQ=255)                                                                                      aCCAGAAACTGTTCAAGGGGTAACTAAAAAAGCCAAATTAGAAAACTGGGAAAGACGACTTATAAAAGGAAGAAAAGGGAAAACATACGAATACCATTATTTATCCATGCCTTACGAGGTGCAGCGCGCCCTAGGCTTTAAACGCAATATAATAGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAAt                                                                                                                                                               >  1:635266/1‑249 (MQ=255)                                                                                      aCCAGAAACTGTTCAAGGGGTAACTAAAAAAGCCAAATTAGAAAACTGGGAAAGACGACTTATAAAAGGAAGAAAAGGGAAAACATACGAATACCATTATTTATCCATGCCTTACGAGGTGCAGCGCGCCCTAGGCTTTAAACGCAATATAATAGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGAATTTTAACGGCGATCAGCACACTTGAAGAAAt                                                                                                                                                               >  1:320736/1‑249 (MQ=255)                                                                                              cTGTTCAAGGGGTAACTAAAAAAGCCAAATTAGAAAACTGGGAAAGACGACTTATAAAAGGAAGAAAAGGGAAAACATACGAATACCATTATTTATCCATGCCTTACGAGGTGCAGCGCGCCCTAGGCTTTAAACGCAATATAATAGAAGAGTCTACCGCACAatat                                                                                                                                                                                                                                         <  1:884690/167‑1 (MQ=255)                                                                                                gTTCAAGGGGTAACTAAAAAAGCCAAATTAGAAAACTGGGAAAGACGACTTATAAAAGGAAGAAAAGGGAAAACATACGAATACCATTATTTATCCATGCCTTACGAGGTGCAGCGCGCCCTAGGCTTTAAACGCAATATAATAGAAGAGTCTACCGCACAATATAACGCATTAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGa                                                                                                                                                    >  1:147169/1‑250 (MQ=255)                                                                                                gTTCAAGGGGTAACTAAAAAAGCCAAATTAGAAAACTGGGAAAGACGACTTATAAAAGGAAGAAAAGGGAAAACATACGAATACCATTATTTATCCATGCCTTACGAGGTGCAGCGCGCCCTAGGCTTTAAACGCAATATAATAGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGa                                                                                                                                                    >  1:338539/1‑250 (MQ=255)                                                                                                                       cAAATTAGAAAACTGGGAAAGACGACTTATAAAAGGAAGAAAAGGGAAAACATACGAATACCATTATTTATCCATGCCTTACGAGGTGCAGCGCGCCCTAGGCTTTAAACGCAATATAATAGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCacac                                                                                                                                                                         <  1:888767/206‑1 (MQ=255)                                                                                                                         aaTTAGAAAACTGGGAAAGACGACTTATAAAAGGAAGAAAAGGGAAAACATACGAATACCATTATTTATCCATGCCTTACGAGGTGCAGCGCGCCCTAGGCTTTAAACGCAATATAATAGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAAc                                                                                                                                          >  1:692607/1‑235 (MQ=255)                                                                                                                          aTTAGAAAACTGGGAAAGACGACTTATAAAAGGAAGAAAAGGGAAAACATACGAATACCATTATTTATCCATGCCTTACGAGGTGCAGCGCGCCCTAGGCTTTAAACGCAATATAATAGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCa                                                                                                                         >  1:830752/1‑251 (MQ=255)                                                                                                                           ttAGAAAACTGGGAAAGACGACTTATAAAAGGAAGAAAAGGGAAAACATACGAATACCATTATTTATCCATGCCTTACGAGGTGCAGCGCGCCCTAGGCTTTAAACGCAATATAATAGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCaa                                                                                                                        >  1:319097/1‑251 (MQ=255)                                                                                                                                 aaCTGGGAAAGACGACTTATAAAAGGAAGAAAAGGGAAAACATACGAATACCATTATTTATCCATGCCTTACGAGGTGCAGCGCGCCCTAGGCTTTAAACGCAATATAATAGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCa                                                                                                                  >  1:464/1‑251 (MQ=255)                                                                                                                                                              aaaaGGGAAAACATACGAATACCATTATTTATCCATGCCTTACGAGGTGCAGCGCGCCCTAGGCTTTAAACGCAATATAATAGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTa                                                                                      <  1:189513/250‑1 (MQ=255)                                                                                                                                                                    gAAAACATACGAATACCATTATTTATCCATGCCTTACGAGGTGCAGCGCGCCCTAGGCTTTAAACGCAATATAATAGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGaa                                                                                            >  1:729569/1‑238 (MQ=255)                                                                                                                                                                      aaaCATACGAATACCATTATTTATCCATGCCTTACGAGGTGCAGCGCGCCCTAGGCTTTAAACGCAATATAATAGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCgaagaa                                                                             >  1:112038/1‑251 (MQ=255)                                                                                                                                                                        aCATACGAATACCATTATTTATCCATGCCTTACGAGGTGCAGCGCGCCCTAGGCTTTAAACGCAATATAATAGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAg                                                                           >  1:47706/1‑251 (MQ=255)                                                                                                                                                                                   ccATTATTTATCCATGCCTTACGAGGTGCAGCGCGCCCTAGGCTTTAAACGCAATATAATAGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGaa                                                                                            >  1:823092/1‑223 (MQ=255)                                                                                                                                                                                      ttatttatCCATGCCTTACGAGGTGCAGCGCGCCCTAGGCTTTAAACGCAATATAATAGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAGCGCTAACGagaaga                                                             >  1:332880/1‑251 (MQ=255)                                                                                                                                                                                      ttatttatCCATGCCTTACGAGGTGCAGCGCGCCCTAGGCTTTAAACGCAATATAATAGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAGCGCTAACGagaag                                                              <  1:470275/250‑1 (MQ=255)                                                                                                                                                                                       tatttatCCATGCCTTACGAGGTGCAGCGCGCCCTAGGCTTTAAACGCAATATAATAGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAGCGCTAACGAGAAGat                                                            >  1:694608/1‑251 (MQ=255)                                                                                                                                                                                                  gCCTTACGAGGTGCAGCGCGCCCTAGGCTTTAAACGCAATATAATAGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAGCGCTAACGAGAAGATTATTAATTTAg                                                 >  1:572260/1‑251 (MQ=255)                                                                                                                                                                                                            gTGCAGCGCGCCCTAGGCTTTAAACGCAATATAATAGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGaa                                                                                            >  1:493123/1‑198 (MQ=255)                                                                                                                                                                                                            gTGCAGCGCGCCCTAGGCTTTAAACGCAATATAATAGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAGCGCTAACGAGAAGATTATTAAt                                                     >  1:878318/1‑237 (MQ=255)                                                                                                                                                                                                                     gcCCTAGGCTTTAAACGCAATATAATAGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAGCGCTAACGAGAAGATTATTAATTTAGTTAAATTGGTTGCATaaa                               >  1:415189/1‑250 (MQ=255)                                                                                                                                                                                                                      cccTAGGCTTTAAACGCAATATAATAGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACTCGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGgatgat                                                                                                   <  1:767249/181‑1 (MQ=255)                                                                                                                                                                                                                      cccTAGGCTTTAAACGCAATATAATAGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAGCGCTAACGAGAAGATTATTAATTTAGTTAAATTGGTTGCATaaa                               >  1:723616/1‑249 (MQ=255)                                                                                                                                                                                                                            gCTTTAAACGCAATATAATAGAAGAGTCTACCGCACAATATAACGCATCAAAAAAAGACTTAACGGAAGTAAGc                                                                                                                                                                                                        <  1:517443/74‑1 (MQ=255)                                                                                                                                                                                                                             cTTTAAACGCAATATAATAGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAGCGCTAACGAGAAGATTATTAATTTAGTTAAATTGGTTGCATAAACTAAGgag                       <  1:852450/250‑1 (MQ=255)                                                                                                                                                                                                                                   aCGCAATATAATAGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGcc                                                                                                                          >  1:286589/1‑145 (MQ=255)                                                                                                                                                                                                                                      cAATATAATAGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTa                                                                                                                                                                                                           >  1:81697/1‑61 (MQ=255)                                                                                                                                                                                                                                      cAATATAATAGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAGCGCTAACGAGAAGATTATTAATTTAGTTAAATTGGTTGCATAAACTAAGGAGAGTTATGGa              >  1:854002/1‑250 (MQ=255)                                                                                                                                                                                                                                        atatAATAGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATgg                                                                                                             >  1:285794/1‑153 (MQ=255)                                                                                                                                                                                                                                        atatAATAGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAGCGCTAACGAGAAGATTATTAATTTAGTTAAATTGGTTGCATAAACTAAGGAGAGTTATGGAc             >  1:876946/1‑249 (MQ=255)                                                                                                                                                                                                                                          ataataGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAGCGCTAACGAGAAGATTATTAATTTAGTTAAATTGGTTGCATAAACTAAGGAGAGTTATGGACAAg          >  1:253706/1‑250 (MQ=255)                                                                                                                                                                                                                                           taataGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGaaa                                                                            >  1:833169/1‑183 (MQ=255)                                                                                                                                                                                                                                           taataGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAGCGCTAACGAGAAGATTATTAATTTAGTTAAATTGGTTGCATAAACTAAGGAGAGTTATGGACAAGCa        >  1:218147/1‑251 (MQ=255)                                                                                                                                                                                                                                           taataGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAGCGCTAACGAGAAGATTATTAATTTAGTTAAATTGGTTGCATAAACTAAGGAGAGTTATGGACAAGCa        >  1:752460/1‑251 (MQ=255)                                                                                                                                                                                                                                           taataGAAGAGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAGCGCTAACGAGAAGATTATTAATTTAGTTAAATTGGTTGCATAAACTAAGGAGAGTTATGGACAAGCa        >  1:23326/1‑251 (MQ=255)                                                                                                                                                                                                                                                 aagaGTCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCCGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAGCGCTAACGAGAAGATTATTAATTTAGTTAAATTGGTTGCATAAACTAAGGAGAGTTATGGACAAGCAGTCAAt  <  1:185669/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      GTTTTAATCCTTGGTTTAAAACTAGGAGGTTAGATATGATCCGAGAGTGGCTAGAACTGAAAGACCTTTTGGGAGTAGCTGGACTACCAGAAACTGTTCAAGGGGTAACTAAAAAAGCCAAATTAGAAAACTGGGAAAGACGACTTATAAAAGGAAGAAAAGGGAAAACATACGAATACCATTATTTATCCATGCCTTACGAGGTGCAGCGCGCCCTAGGCTTTAAACGCAATATAATAGAAGAGCCTACCGCACAATATAACGCATCAAAAGAAGACTTAACGGAAGTAAGCAAGGCGCGCTTTTTAACGGCGATCAGCACACTTGAAGAAATCTTAGCAATGACACGCAAAACCATGGAGCCAGAAGCCAAGGCGCAAATGGTATGGATGATTTATGAATTACTAAGCGAAGAAAGCGCTAACGAGAAGATTATTAATTTAGTTAAATTGGTTGCATAAACTAAGGAGAGTTATGGACAAGCAGTCAAT  >  1/1255146‑1255636

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑