Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 1,254,714 G→A intergenic (+115584/+138597) unknown → / ← unknown tRNA‑Met‑CAT/tRNA‑Val‑GAC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*11,254,7140GA100.0% 111.4 / NA 40intergenic (+115584/+138597)unknown/unknowntRNA‑Met‑CAT/tRNA‑Val‑GAC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/16);  total (24/16)

TAATAGATGAAAATGCTTTCTTTATATGTAATAAATAATCTTTTTTCTGCTTTGTATCATGAGTGTTTATAGCAATTTGATATTTAAATGATTCTAAGGAATCAATACCAAATAACGAAACATAAGAATACTTATTCATTTTGAAATTATTATGATTCTTTTCATAGAAATTCTTCCAGAAATGCGTTTTACCAATTCCCCATTCACCTGTAAGTGCAGTAGCAAAAGAGTCTGCTTGATTGATAAGGTTTAATAATTTTTCTTCAATTTTTTCAGTAGTTGCCATAATTGGTTACTCTAAATGATCCATTCTCCCTTCGGATAACTAACTCGCAATGCATTTTTTAATGTGGAGGGAGCCAATCCAGACTTAACTGAAAGCGAATGCAATGTAATGTTTCGCTCATGTAACTCGTAAACAATCCGCTTTCTACTCCAATCCTTTTTTTTCATAGTTCCTCG  >  1/1254496‑1254957
                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                   
taataGATGAAAATGCTTTCTTTATATGTAATAAATAATCTTTTTTCTGCTTTGTATCATGAGTGTTTATAGCAATTTGATATTTAAATGATTCTAAGGAATCAATACCAAATAACGAAACATAAGAATACTTATTCATTTTGAAATTATTATGATTCTTTTCATAGAAATTCTTCCAGAAATGCGTTTTACCAATTCCCCATTCACCTGTAAGTGCAATAGCAAAAGAGTCTGCTTGATTGATAAGGttt                                                                                                                                                                                                                     >  1:158180/1‑251 (MQ=255)
                   ctttATATGTAATAAATAATCTTTTTTCTGCTTTGTATCATGAGTGTTTATAGCAATTTGATATTTAAATGATTCTAAGGAATCAATACCAAATAACGAAACATAAGAATACTTATTCATTTTGAAATTATTATGATTCTTTTCATAGAAATTCTTCCAGAAATGCGTTTTACCAATTCCCCATTCACCTGTAAGTGCAATAGCAAAAGAGTCTGCTTGATTGATAAGGTTTAATAATTTTTCTTCAAttt                                                                                                                                                                                                  >  1:558871/1‑251 (MQ=255)
                                  ataatCTTTTTTCTGCTTTGTATCATGAGTGTTTATAGCAATTTGATATTTAAATGATTCTAAGGAATCAATACCAAATAACGAAACATAAGAATACTTATTCATTTTGAAATTATTATGATTCTTTTCATAGAAATTCTTCCAGAAATGCGTTTTACCAATTCCCCATTCACCTGTAAGTGCAATAGCAAAAGAGTCTGCttgat                                                                                                                                                                                                                                <  1:729162/206‑1 (MQ=255)
                                     atCTTTTTTCTGCTTTGTATCATGAGTGTTTATAGCAATTTGATATTTAAATGATTCTAAGGAATCAATACCAAATAACGAAACACAAGAATACTTATTCATTTTGAAATTATTATGATTCTTTTCATAGAAATTCTTCCAGAAATGCGTTTTACCAATTCCCCATTCACCTGTAAGTGCAATAGCAAAAGAGTCTGCTTGATTGATAAGGTTTAATAATTTTTCTTCAATTTTTTCAGTAGTTGCCATaa                                                                                                                                                                                >  1:545540/1‑251 (MQ=255)
                                                             aGTGTTTATAGCAATTTGATATTTAAATGATTCTAAGGAATCAATACCAAATAACGAAACATAAGAATACTTATTCATTTTGAAATTATTATGATTCTTTTCATAGAAATTCTTCCAGAAATGCGTTTTACCAATTCCCCATTCACCTGTAAGTGCAATAGCAAAAGAGTCTGCTTGATTGATAAGGTTTAATAATTTTTCTTCAATTTTTTCagt                                                                                                                                                                                           >  1:553330/1‑216 (MQ=255)
                                                                gtTTATAGCAATTTGATATTTAAATGATTCTAAGGAATCAATACCAAATAACGAAACATAAGAATACTTATTCATTTTGAAATTATTATGATTCTTTTCATAGAAATTCTTCCAGAAATGCGTTTTACCAATTCCCCATTCACCTGTAAGTGCAATAGCAAAAGAGTCTGCTTGATTGATAAGGTTTAATAATTTTTCTTCAATTTTTTCAGTAGTTGCCATAATTGGTTACTCTAAATGATCCATtctc                                                                                                                                                      >  1:737754/1‑250 (MQ=255)
                                                                    ataGCAATTTGATATTTAAATGATTCTAAGGAATCAATACCAAATAACGAAACATAAGAATACTTATTCATTTTGAAATTATTATGATTCTTTTCATAGAAATTCTTCCAGAAATGCGTTTTACCAATTCCCCATTCACCTGTAAGTGCAATAGCAAAAGAGTCTGCTTGATTGATAAGGTTTAATAATTTTTCTTCAATTTTTTCAGTAGTTGCCATAATTGGTTACTCTAAATGATCCATTCTCCCTTc                                                                                                                                                 <  1:391973/251‑1 (MQ=255)
                                                                        cAATTTGATATTTAAATGATTCTAAGGAATCAATACCAAATAACGAAACATAAGAATACTTATTCATTTTGAAATTATTATGATTCTTTTCATAGAAATTCTTCCAGAAATGCGTTTTACCAATTCCCCATTCACCTGTAAGTGCAATAGCAAAAGAGTCTGCTTGATTGATAAGGTTTAATAATTTTTCTTCAATTTTTTCAGTAGTTGCCATAATTGGTTACTCTAAATGATCCATTCTCCCTTCGGa                                                                                                                                              <  1:169220/250‑1 (MQ=255)
                                                                                          aTTCTAAGGAATCAATACCAAATAACGAAACATAAGAATACTTATTCATTTTGAAATTATTATGATTCTTTTCATAGAAATTCTTCCAGAAATGCGTTTTACCAATTCCCCATTCACCTGTAAGTGCAATAGCAAAAGAGTCTGCttgatt                                                                                                                                                                                                                               >  1:488062/1‑151 (MQ=255)
                                                                                          aTTCTAAGGAATCAATACCAAATAACGAAACATAAGAATACTTATTCATTTTGAAATTATTATGATTCTTTTCATAGAAATTCTTCCAGAAATGCGTTTTACCAATTCCCCATTCACCTGTAAGTGCAATAGCAAAAGAGTCTGCTTGATTGATAAGGTTTAATAATTTTTCTTCAATTTTTTCAGTAGTTGCCATAATTGGTTACTCTAAATGATCCATtctc                                                                                                                                                      <  1:162474/224‑1 (MQ=255)
                                                                                          aTTCTAAGGAATCAATACCAAATAACGAAACATAAGAATACTTATTCATTTTGAAATTATTATGATTCTTTTCATAGAAATTCTTCCAGAAATGCGTTTTACCAATTCCCCATTCACCTGTAAGTGCAATAGCAAAAGAGTCTGCTTGATTGATAAGGTTTAATAATTTTTCTCCAATTTTTTCAGTAGTTGCCATAATTGGTTACTCTAAATGATCCATTCTCCCTTCGGATAACTAACTCGCAATGCAt                                                                                                                           >  1:821184/1‑251 (MQ=255)
                                                                                                    aTCAATACCAAATAACGAAACATAAGAATACTTATTCATTTTGAAATTATTATGATTCTTTTCATAGAAATTCTTCCAGAAATGCGTTTTACCAATTCCCCATTCACCTGTAAGTGCAATAGCAAAAGAGTCTGCTTGATTGATAAGGTTTAATAATTTTTCTTCAATTTTTTCAGTAGTTGCCATAATTGGTTACTCTAAAt                                                                                                                                                                 <  1:845244/203‑1 (MQ=255)
                                                                                                    aTCAATACCAAATAACGAAACATAAGAATACTTATTCATTTTGAAATTATTATGATTCTTTTCATAGAAATTCTTCCAGAAATGCGTTTTACCAATTCCCCATTCACCTGTAAGTGCAATAGCAAAAGAGTCTGCTTGATTGATAAGGTTTAATAATTTTTCTTCAATTTTTTCAGTAGTTGCCATAATTGGTTACTCTAAATGAt                                                                                                                                                              >  1:382603/1‑206 (MQ=255)
                                                                                                        aTACCAAATAACGAAACATAAGAATACTTATTCATTTTGAAATTATTATGATTCTTTTCATAGAAATTCTTCCAGAAATGCGTTTTACCAATTCCCCATTCACCTGTAAGTGCAATAGCAAAAGAGTCTGCTTGATTGATAAGGTTTAATAATTTTTCTTCAATTTTTTCAGTAGTTGCCATAATTGGTTACTCTAAATg                                                                                                                                                                <  1:641552/200‑1 (MQ=255)
                                                                                                          aCCAAATAACGAAACATAAGAATACTTATTCATTTTGAAATTATTATGATTCTTTTCATAGAAATTCTTCCAGAAATGCGTTTTACCAATTCCCCATTCACCTGTAAGTGCAATAGCAAAAGAGTCTGCTTGATTGATAAGGTTTAATAATTTTTCTTCAATTTTTTCAGTAGTTGCCATAATTGGTTACTCTAAATGATCCATTCTCCCTTCGGATAACTAACTCGCAATGCATTTTTTAATGTggaggg                                                                                                           >  1:527454/1‑251 (MQ=255)
                                                                                                                tAACGAAACATAAGAATACTTATTCATTTTGAAATTATTATGATTCTTTTCATAGAAATTCTTCCAGAAATGCGTTTTACCAATTCCCCATTCACCTGTAAGTGCAATAGCAAAAGAGTCTGCTTGATTGATAAGGTTTAATAATTTTTCTTCAATTTTTTCAGTAGTTGCCATAATTGGTTACTCTAAATGATCCATTCTCCCTTCGGATAACTAACTCGCAATGCATTTTTTAATGTGGAGGGAGCCaa                                                                                                     >  1:516325/1‑251 (MQ=255)
                                                                                                                     aaaCATAAGAATACTTATTCATTTTGAAATTATTATGATTCTTTTCATAGAAATTCTTCCAGAAATGCGTTTTACCAATTCCCCATTCACCTGTAAGTGCAATAGCAAAAGAGTCTGCTTGATTGATAAGGTTtaata                                                                                                                                                                                                                 >  1:870926/1‑138 (MQ=255)
                                                                                                                        cATAAGAATACTTATTCATTTTGAAATTATTATGATTCTTTTCATAGAAATTCTTCCAGAAATGCGTTTTACCAATTCCCCATTCACCTGTAAGTGCAATAGCAAAAGAGTCTGCTTGATTGATAAGGTTTAATAATTTTTCTTCAATTTTTTCAGTAGTTGCCATAATTGGTTACTCTAAATGATCCATTCTCCCTTCGGATAACTAACTCGCAATGCATTTTTTAATGTGGAGGGAGCCAATCCAGa                                                                                               >  1:114073/1‑249 (MQ=255)
                                                                                                                        cATAAGAATACTTATTCATTTTGAAATTATTATGATTCTTTTCATAGAAATTCTTCCAGAAATGCGTTTTACCAATTCCCCATTCACCTGTAAGTGCAATAGCAAAAGAGTCTGCTTGATTGATAAGGTTTAATAATTTTTCTTCAATTTTTTCAGTAGTTGCCATAATTGGTTACTCTAAATGACCCATTCTCCCTTCGGATAACTAACTCGCAATGCATTTTTTAATGTGGAGGGAGCCAATCCAGa                                                                                               >  1:103011/1‑249 (MQ=255)
                                                                                                                          tAAGAATACTTATTCATTTTGAAATTATTATGATTCTTTTCATAGAAATTCTTCCAGAAATGCGTTTTACCAATTCCCCATTCACCTGTAAGTGCAATAGCAAAAGAGTCTGCTTGATTGATAAGGTTTAATAATTTTTCTTCAATTTTTTCagt                                                                                                                                                                                           <  1:464768/155‑1 (MQ=255)
                                                                                                                          tAAGAATACTTATTCATTTTGAAATTATTATGATTCTTTTCATAGAAATTCTTCCAGAAATGCGTTTTACCAATTCCCCATTCACCTGTAAGTGCAATAGCAAAAGAGTCTGCTTGATTGATAAGGTTTAATAATTTTTCTTCAATTTTTTCAGTAGTTGCCATAATTGGTTACTCTAAATGATCCATtctc                                                                                                                                                      <  1:828964/192‑1 (MQ=255)
                                                                                                                                               aaaTTATTATGATTCTTTTCATAGAAATTCTTCCAGAAATGCGTTTTACCAATTCCCCATTCACCTGTAAGTGCAATAGCAAAAGAGTCTGCTTGATTGATAAGGTTTAATAATTTTTCTTCAATTTTTTCAGTAGTTGCCATAATTGGTTACTCTAAATGATCCATTCTCCCTTCGGATAACTAACTCGCAATGCATTTTTTAATGTGGAGGGAGCCAATCCAGACTTAACTGAAAGCGAATGCaatgt                                                                       <  1:311202/250‑1 (MQ=255)
                                                                                                                                                 attattATGATTCTTTTCATAGAAATTCTTCCAGAAATGCGTTTTACCAATTCCCCATTCACCTGTAAGTGCAATAGCAAAAGAGTCTGCTTGATTGATAAGGTTTAATAATTTTTCTTCAATTTTTTCAGTAGTTGCCATAATTGGTTACTCTAAATGATCCATtctc                                                                                                                                                      <  1:677812/169‑1 (MQ=255)
                                                                                                                                                     ttatGATTCTTTTCATAGAAATTCTTCCAGAAATGCGTTTTACCAATTCCCCATTCACCTGTAAGTGCAATAGCAAAAGAGTCTGCTTGATTGATAAGGTTTAATAATTTTTCTTCAATTTTTTCAGTAGTTGCCATAATTGGTTACTCTAAATg                                                                                                                                                                >  1:879286/1‑155 (MQ=255)
                                                                                                                                                     ttatGATTCTTTTCATAGAAATTCTTCCAGAAATGCGTTTTACCAATTCCCCATTCACCTGTAAGTGCAATAGCAAAAGAGTCTGCTTGATTGATAAGGTTTAATAATTTTTCTTCAATTTTTTCAGTAGTTGCCATAATTGGTTACTCTAAATGATCCATTCTCCCTTCGGATAACTAACTCGCAATGCATTTTTTAATGTGGAGGGAGCCAATCCAGACTTAACTGAAAGCGAATGCAATGTAATGttt                                                                >  1:773902/1‑251 (MQ=255)
                                                                                                                                                     ttatGATTCTTTTCATAGAAATTCTTCCAGAAATGCGTTTTACCAATTCCCCATTCACCTGTAAGTGCAATAGCAAAAGAGTCTGCTTGATTGATAAGGTTTAATAATTTTTCTTCAATTTTTTCAGTAGTTGCCATAATTGGTTACTCTAAATGATCCATTCTCCCTTCGGATAACTAACTCGCAATGCATTTTTTAATGTGGAGGGAGCCAATCCAGACTTAACTGAAAGCGAATGCAATGTAATGttt                                                                <  1:184885/251‑1 (MQ=255)
                                                                                                                                                                         aTTCTTCCAGAAATGCGTTTTACCAATTCCCCATTCACCTGTAAGTGCAATAGCAAAAGAGTCTGCTTGATTGATAAGGTTTAATAATTTTTCTTCAATTTTTTCAGTAGTTGCCATAATTGGTTACTCTAAATGATCCATTCTCCCTTCGGATAACTAACTCGCAATGCATTTTTTAATGTGGAGGGAGCCAATCCAGACTTAACTGAAAGCGAATGCAATGTAATGTTTCGCTCATGTAACTCGTAAAc                                            <  1:613601/251‑1 (MQ=255)
                                                                                                                                                                             ttcCAGAAATGCGTTTTACCAATTCCCCATTCACCTGTAAGTGCAATAGCAAAAGAGTCTGCTTGATTGATAAGGTTTAATAATTTTTCTTCAATTTTTTCAGTAGTTGCCATAATTGGTTACTCTAAATGATCCATTCTCCCTTCGGATAACTAACTCGCAATGCATTTTTTAATGTGGAGGGAGCCAATCCAGACt                                                                                             <  1:613128/198‑1 (MQ=255)
                                                                                                                                                                                  gAAATGCGTTTTACCAATTCCCCATTCACCTGTAAGTGCAATAGCAAAAGAGTCTGCTTGATTGATAAGGTTTAATAATTTTTCTTCAATTTTTTCAGTAGTTGCCATAATTGGTTACTCTAAATGATCCATTCTCCCTTCGGATAACTAACTCGCAATGCATTTTTTAATGTGGAGGGAGCCAATCCAGACTTAACTGAAAGCGAATGCaatgtaatgt                                                                  >  1:626132/1‑220 (MQ=255)
                                                                                                                                                                                       gCGTTTTACCAATTCCCCATTCACCTGTAAGTGCAATAGCAAAAGAGTCTGCTTGATTGATAAGGTTTAATAATTTTTCTTCAATTTTTTCAGTAGTTGCCATAATTGGTTACTCTAAATGATCCATTCTCCCTTCGGATAACTAACTCGCAATGCATTTTTTAATGTGGAGGGAGCCAATCCAGACt                                                                                             <  1:221044/188‑1 (MQ=255)
                                                                                                                                                                                             tACCAATTCCCCATTCACCTGTAAGTGCAATAGCAAAAGAGTCTGCTTGATTGATAAGGTTTAATAATTTTTCTTCAATTTTTTCAGTAGTTGCCATAATTGGTTACTCTAAATGATCCATTCTCCCTTCGGATAACTAACTCGCAATGCATTTTTTAATGTGGAGGGAGCCAATCCAGACTTAACTGAAAGCGAATGCAATGTAATGttt                                                                <  1:102694/211‑1 (MQ=255)
                                                                                                                                                                                                   ttCCCCATTCACCTGTAAGTGCAATAGCAAAAGAGTCTGCTTGATTGATAAGGTTTAATAATTTTTCTTCAATTTTTTCAGTAGTTGCCATAATTGGTTACTCTAAATGATCCATTCTCCCTTCGGATAACTAACTCGCAATGCATTTTTTAATGTGGAGGGAGCCAATCCAGACTTAACTGAAAGCGAATGCAATGTAATGTTTCGCTCATGTAACt                                                   >  1:145754/1‑218 (MQ=255)
                                                                                                                                                                                                       ccATTCACCTGTAAGTGCAATAGCAAAAGAGTCTGCTTGATTGATAAGGTTTAATAATTTTTCTTCAATTTTTTCAGTAGTTGCCATAATTGGTTACTCTAAATGATCCATTCTCCCTTCGGATAACTAACTCGCAATGCATTTTTTAATGTGGAGGGAGCCAATCCAGACTTAACTGAAAGCGAATGCAATGTAATGTTTCGCTCATGTAACTCGTAAACAATCCGCTTTCTACTCCAATCCtttttttt              >  1:38287/1‑251 (MQ=255)
                                                                                                                                                                                                              ccTGTAAGTGCAATAGCAAAAGAGTCTGCTTGATTGATAAGGTTTAATAATTTTTCTTCAATTTTTTCAGTAGTTGCCATAATTGGTTACTCTAAATGATCCATTCTCCCTTCGGATAACTAACTCGCAATGCATTTTTTAATGTGGAGGGAGCCAATCCAGACTTAACTGAAAGCGAATGCAATGTAATGTTTCGCTCATGTAACTCGTAAACAATCCGCTTTCTACTCCAATCCTTTTTTTTCATAGtt       >  1:680530/1‑251 (MQ=255)
                                                                                                                                                                                                              ccTGTAAGTGCAATAGCAAAAGAGTCTGCTTGATTGATAAGGTTTAATAATTTTTCTTCAATTTTTTCAGTAGTTGCCATAATTGGTTACTCTAAATGATCCATTCTCCCTTCGGATAACTAACTCGCAATGCATTTTTTAATGTGGAGGGAGCCAATCCAGACTTAACTGAAAGCGAATGCAATGTAATGTTTCGCTCATGTAACTCGTAAACAATCCGCTTTCTACTCCAATCCTTTTTTTTCATAGtt       >  1:510180/1‑251 (MQ=255)
                                                                                                                                                                                                                tGTAAGTGCAATAGCAAAAGAGTCTGCTTGATTGATAAGGTTtaat                                                                                                                                                                                                                  >  1:872130/1‑46 (MQ=255)
                                                                                                                                                                                                                 gTAAGTGCAATAGCAAAAGAGTCTGCTTGATTGATAAGGTTTAATAAttt                                                                                                                                                                                                             <  1:638159/50‑1 (MQ=255)
                                                                                                                                                                                                                 gTAAGTGCAATAGCAAAAGAGTCTGCTTGATTGATAAGGTTTAATAATTTTTCTTCAATTTTTTCAGTAGTTGCCATAATTGGTTACTCTAAATGATCCATTCTCCCTTCGGATAACTAACTCGCAATGCATTTTTTAATGTGGAGGGAGCCAATCCAGACTTAACTGAAAGCGAATGCAATGTAATGTTTCGCTCATGTAACTCGTAAACAATCCGCTTTCTACTCCAATCCTTTTTTTTCATAGTTc      >  1:626668/1‑249 (MQ=255)
                                                                                                                                                                                                                     gTGCAATAGCAAAAGAGTCTGCTTGATTGATAAGGTTTAATAATTTTTCTTCAATTTTTTCAGTAGTTGCCATAATTGGTTACTCTAAATGATCCATTCTCCCTTCGGATAACTAACTCGCAATGCATTTTTTAATGTGGAGGGAGCCAATCCAGACTTAACTGAAAGCGAATGCAATGTAATGttt                                                                >  1:680172/1‑187 (MQ=255)
                                                                                                                                                                                                                     gTGCAATAGCAAAAGAGTCTGCTTGATTGATAAGGTTTAATAATTTTTCTTCAATTTTTTCAGTAGTTGCCATAATTGGTTACTCTAAATGATCCATTCTCCCTTCGGATAACTAACTCGCAATGCATTTTTTAATGTGGAGGGAGCCAATCCAGACTTAACTGAAAGCGAATGCAATGTAATGTTTCGCTCATGTAACTCGTAAACAATCCGCTTTCTACTCCAATCCTTTTTTTTCATAGTTCCTCg  >  1:827800/1‑249 (MQ=255)
                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                   
TAATAGATGAAAATGCTTTCTTTATATGTAATAAATAATCTTTTTTCTGCTTTGTATCATGAGTGTTTATAGCAATTTGATATTTAAATGATTCTAAGGAATCAATACCAAATAACGAAACATAAGAATACTTATTCATTTTGAAATTATTATGATTCTTTTCATAGAAATTCTTCCAGAAATGCGTTTTACCAATTCCCCATTCACCTGTAAGTGCAGTAGCAAAAGAGTCTGCTTGATTGATAAGGTTTAATAATTTTTCTTCAATTTTTTCAGTAGTTGCCATAATTGGTTACTCTAAATGATCCATTCTCCCTTCGGATAACTAACTCGCAATGCATTTTTTAATGTGGAGGGAGCCAATCCAGACTTAACTGAAAGCGAATGCAATGTAATGTTTCGCTCATGTAACTCGTAAACAATCCGCTTTCTACTCCAATCCTTTTTTTTCATAGTTCCTCG  >  1/1254496‑1254957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑