Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,250,842	A→G	intergenic (+111712/+142469)	unknown → / ← unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,250,842	0	A	G	100.0%	219.6 / NA	76	intergenic (+111712/+142469)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (38/38);  total (38/38)

CATTTTTTCCGCATTGTGTTTTAGGGGTTGCGGGTGAAGTTTGTCGTAAACCAACAAGCCGATAAAATTAAACCCTTGCGTCAATAAAATAAAGGTTAGGGCATAAACGCCGATTTGCGGTAATTGTTGTGCCAAATCATTCAGTTGACCGAGAGAAAAGCCCATGGAGAACAAGATCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCATCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTAAAATAATGAGCAAACCTTCGTACACCGTGAAAATACCTTAGAGTTATTGTTAAGCGCGAAGTATAGCACTTTTCTGTCACCCCGCCTAATCGCGCGCCGATAAAGCGATTGTTGTATGTTATAATGCCACCCGTTTTTTCTTATTGATTTTCTCCATGACGTTTCAAATTGTATTTCAACATCCTGATT  >  1/1250619‑1251076                                                                                                                                                                                                                                |                                                                                                                                                                                                                                           cATTTTTTCCGCATTGTGTTTTAGGGGTTGCGGGTGAAGTTTGTCGTAAACCAACAAGCCGATAAAATTAAACCCTTGCGTCAATAAAATAAAGGTTAGGGCATAAACGCCGATTTGCGGTAATTGTTGTGCCAAATCATTCAGTTGACCGAGAGAAAAGCCCATGGAGAACAAGATCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAg                                                                                                                                                                                                                  >  1:179160/1‑250 (MQ=255) cATTTTTCCCGCATTGTGTTTTAGGGGTTGCGGGTGAAGTTTGTCGTAAACCAACAAGCCGATAAAATTAAACCCTTGCGTCAATAAAATAAAGGTTAGGGCATAAACGCCGATTTGCGGTAATTGTTGTGCCAAATCATTCAGTTGACCGAGAGAAAAGCCCATGGAGAACAAGATCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGc                                                                                                                                                                                                                 <  1:133452/251‑1 (MQ=255)           gCATTGTGTTTTAGGGGTTGCGGGTGAAGTTTGTCGTAAACCAACAAGCCGATAAAATTAAACCCTTGCGTCAATAAAATAAAGGTTAGGGCATAAACGCCGATTTGCGGTAATTGTTGTGCCAAATCATTCAGTTGACCGAGAGAAAAGCCCATGGAGAACAAGATCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACAt                                                                                                                                                                                                       >  1:703796/1‑251 (MQ=255)                gtgtTTTAGGGGTTGCGGGTGAAGTTTGTCGTAAACCAACAAGCCGATAAAATTAAACCCTTGCGTCAATAAAATAAAGGTTAGGGCATAAACGCCGATTTGCGGTAATTGTTGTGCCAAATCATTCAGTTGACCGAGAGAAAAGCCCATGGAGAACAAGATCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCa                                                                                                                                                                                                  >  1:395246/1‑251 (MQ=255)                gtgtTTTAGGGGTTGCGGGTGAAGTTTGTCGTAAACCAACAAGCCGATAAAATTAAACCCTTGCGTCAATAAAATAAAGGTTAGGGCATAAACGCCGATTTGCGGTAATTGTTGTGCCAAATCATTCAGTTGACCGAGAGAAAAGCCCATGGAGAACAAGATCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCa                                                                                                                                                                                                  >  1:569370/1‑251 (MQ=255)                gtgtTTTAGGGGTTGCGGGTGAAGTTTGTCGTAAACCAACAAGCCGATAAAATTAAACCCTTGCGTCAATAAAATAAAGGTTAGGGCATAAACGCCGATTTGCGGTAATTGTTGTGCCAAATCATTCAGTTGACCGAGAGAAAAGCCCATGGAGAACAAGATCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCa                                                                                                                                                                                                  >  1:358526/1‑251 (MQ=255)                          gttagCGGGTAAAGTTTGTCGTAAACCACCAAGCCGATAAAATTAAACCCTTGCATCAATAAAATAAAGGTTAAGGCATAAACGCCGATTTGCGGTAATTGTTGTGCCAAATCATTCAGTTGACCGAGAGAAAAGCCCATGGAGAACAAGATCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTAAaataa                                                                                                                                                                                         <  1:347199/246‑1 (MQ=255)                           aaacaggttGAAGTTTGTCTTAAACCAACAAGCCGATAAAATTAAACCCTTGCGTCAATAAAATAAAGGTTAGGGCATAAACGCCGATTTGCGGTAATTGTTGTGCCAAATCATTCAGTTGACCGAGAGAAAAGCCCATGGAGAACAAGATCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTAAaataa                                                                                                                                                                                         <  1:420094/241‑1 (MQ=255)                             tGCGGGTGAAGTTTGTCGAAAACAAACAAGCCGATAAAATTAAACCCTTGCGTCAATAAAATAAAGGTTAGGGCATAAACGCCGATTTGCGGTAATTGTTGTGCCAAATCATTCAGTTGACCGAGAGAAAAGCCCATGGAGAACAAGATCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTAAAATAATGAg                                                                                                                                                                                     <  1:281519/251‑1 (MQ=255)                                gggTGAAGTTTGTCGTAAACCAACAAGCCGATAAAATTAAACCCTTGCGTCAATAAAATAAAGGTTAGGGCATAAACGCCGATTTGCGGTAATTGTTGTGCCAAATCATTCAGTTGACCGAGAGAAAAGCCCATGGAGAACAAGATCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTAAAATAATGAGCaa                                                                                                                                                                                  >  1:28617/1‑251 (MQ=255)                                 ggTGAAGTTTGTCGTAAACCAACAAGCCGATAAAATTAAACCCTTGCGTCAATAAAATAAAGGTTAGGGCATAAACGCCGATTTGCGGTAATTGTTGTGCCAAATCATTCAGTTGACCGAGAGAAAAGCCCATGGAGAACAAGATCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTAAAATAATGAGCaaa                                                                                                                                                                                 >  1:626300/1‑251 (MQ=255)                                      aGTTTGTCGTAAACCAACAAGCCGATAAAATTAAACCCTTGCGTCAATAAAATAAAGGTTAGGGCATAAACGCCGATTTGCGGTAATTGTTGTGCCAAGTCATTCAGTTGACCGAGAGAAAAGCCCATGGAGAACAAGATCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTAAAATAATGAGCAAACCtt                                                                                                                                                                             >  1:280586/1‑250 (MQ=255)                                      aGTTTGTCGTAAACCAACAAGCCGATAAAATTAAACCCTTGCGTCAATAAAATAAAGGTTAGGGCATAAACGCCGATTTGCGGTAATTGTTGTGCCAAATCATTCAGTTGACCGAGAGAAAAGCCCATGGAGAACAAGATCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAAc                                                                                                                                                                                                                         <  1:402978/206‑1 (MQ=255)                                      aGTTTGTCGTAAACCAACAAGCCGATAAAATTAAACCCTTGCGTCAATAAAATAAAGGTTAGGGCATAAACGCCGATTTGCGGTAATTGTTGTGCCAAATCATTCAGTTGACCGAGAGAAAAGCCCATGGAGAACAAGATCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTAAaataa                                                                                                                                                                                         >  1:143218/1‑238 (MQ=255)                                          tgtggTAAACAAACAAGCCGATAAAATTAAACCCTTGCGTCAATAAAATAAAGGTTAGGGCATAAACGCCGATTTGCGGTAATTGTTGTGCCAAATCATTCAGTTGACCGAGAGAAAAGCCCATGGAGAACAAGATCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTAAAATAATGAGCAAACCTTCGTac                                                                                                                                                                        <  1:438744/247‑1 (MQ=255)                                          tGTCGTAAACCAACAAGCCGATAAAATTAAACCCTTGCGTCAATAAAATAAAGGTTAGGGCATAAACGCCGATTTGCGGTAATTGTTGTGCCAAATCATTCAGTTGACCGAGAGAAAAGCCCATGGAGAACAAGATCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTAAAATAATGAGCAAACCTTCGTac                                                                                                                                                                        <  1:323548/251‑1 (MQ=255)                                            tCGTAAACCAACAAGCCGATAAAATTAAACCCTTGCGTCAATAAAATAAAGGTTAGGGCATAAACGCCGATTTGCGGTAATTGTTGTGCCAAATCATTCAGTTGACCGAGAGAAAAGCCCATGGAGAACAAGATCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTAAAATAATGAGCAAACCTTCGTacac                                                                                                                                                                      <  1:390544/251‑1 (MQ=255)                                                          gCCGATAAAATTAAACCCTTGCGTCAATAAAATAAAGGTTAGGGCATAAACGCCGATTTGCGGTAATTGTTGTGCCAAATCATTCAGTTGACCGAGAGAAAAGCCCATGGAGAACAAGATCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTAAAATAATGAGCAAACCTTCGTACACCGTGAAAATACCtt                                                                                                                                                        >  1:412198/1‑251 (MQ=255)                                                                 aaaTTAAACCCTTGCGTCAATAAAATAAAGGTTAGGGCATAAACGCCGATTTGCGGTAATTGTTGTGCCAAATCATTCAGTTGACCGAGAGAAAAGCCCATGGAGAACAAGATCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTAAAATAATGAGCAAACCTTCGTACACCGTGAAAATACCTTAGAGTTa                                                                                                                                                 >  1:402001/1‑251 (MQ=255)                                                                 aaaTTAAACCCTTGCGTCAATAAAATAAAGGTTAGGGCATAAACGCCGATTTGCGGTAATTGTTGTGCCAAATCATTCAGTTGACCGAGAGAAAAGCCCATGGAGAACAAGATCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTAAAATAATGAGCAAACCTTCGTACACCGTGAAAATACCTTAGAGTTa                                                                                                                                                 >  1:197642/1‑251 (MQ=255)                                                                  aaTTAAACCCTTGCGTCAATAAAATAAAGGTTAGGGCATAAACGCCGATTTGCGGTAATTGTTGTGCCAAATCATTCAGTTGACCGAGAGAAAAGCCCATGGAGAACAAGATCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGc                                                                                                                                                                                                                 >  1:754783/1‑186 (MQ=255)                                                                  aaTTAAACCCTTGCGTCAATAAAATAAAGGTTAGGGCATAAACGCCGATTTGCGGTAATTGTTGTGCCAAATCATTCAGTTGACCGAGAGAAAAGCCCATGGAGAACAAGATCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTAAAATAATGAGCAAACCTTCGTacac                                                                                                                                                                      >  1:869479/1‑229 (MQ=255)                                                                  aaTTAAACCCTTGCGTCAATAAAATAAAGGTTAGGGCATAAACGCCGATTTGCGGTAATTGTTGTGCCAAATCATTCAGTTGACCGAGAGAAAAGCCCATGGAGAACAAGATCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTAAAATAATGAGCAAACCTTCGTACACCGTGAAAATACCTTAGAGTTAt                                                                                                                                                >  1:634310/1‑251 (MQ=255)                                                                      aaaCCCTTGCGTCAATAAAATAAAGGTTAGGGCATAAACGCCGATTTGCGGTAATTGTTGTGCCAAATCATTCAGTTGACCGAGAGAAAAGCCCATGGAGAACAAGATCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTAAAATAATGAGCAAACCTTCGTACACCGTGAAAATACCTTAGAGTTATTGtt                                                                                                                                            <  1:297266/251‑1 (MQ=255)                                                                         cccTTGCGTCAATACAATAAAGGTTAGGGCATAAACGCCGATTTGCGGTAATTGTTGTGCCAAATCATTCAGTTGACCGAGAGAAAAGCCCATGGAGAACAAGATCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTAAAATAATGAGCAAACCTTCGTACACCGTGAAAATACCTTAGAGTTATTGTTAAg                                                                                                                                         >  1:567972/1‑251 (MQ=255)                                                                         cccTTGCGTCAATAAAATAAAGGTTAGGGCATAAACGCCGATTTGCGGTAATTGTTGTGCCAAATCATTCAGTTGACCGAGAGAAAAGCCCATGGAGAACAAGATCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACaa                                                                                                                                                                                                           <  1:520663/185‑1 (MQ=255)                                                                         cccTTGCGTCAATAAAATAAAGGTTAGGGCATAAACGCCGATTTGCGGTAATTGTTGTGCCAAATCATTCAGTTGACCGAGAGAAAAGCCCATGGAGAACAAGATCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTaaaa                                                                                                                                                                                            >  1:245940/1‑200 (MQ=255)                                                                         cccTTGCGTCAATAAAATAAAGGTTAGGGCATAAACGCCGATTTGCGGTAATTGTTGTGCCAAATCATTCAGTTGACCGAGAGAAAAGCCCATGGAGAACAAGATCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTAAAATAATGAGCAAACCTTCGTacac                                                                                                                                                                      <  1:62629/222‑1 (MQ=255)                                                                         cccTTGCGTCAATAAAATAAAGGTTAGGGCATAAACGCCGATTTGCGGTAATTGTTGTGCCAAATCATTCAGTTGACCGAGAGAAAAGCCCATGGAGAACAAGATCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTAAAATAATGAGCAAACCTTCGTACACCGTGAAAATACCTTAGAGTTATTGTTAAg                                                                                                                                         <  1:709405/251‑1 (MQ=255)                                                                         cccTTGCGTCAATAAAATAAAGGTTAGGGCATAAACGCCGATTTGCGGTAATTGTTGTGCCAAATCATTCAGTTGACCGAGAGAAAAGCCCATGGAGAACAAGATCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTAAAATAATGAGCAAACCTTCGTACACCGTGAAAATACCTTAGAGTTATTGTTAAg                                                                                                                                         >  1:23028/1‑251 (MQ=255)                                                                               cGTCAATAAAATAAAGGTTAGGGCATAAACGCCGATTTGCGGTAATTGTTGTGCCAAATCATTCAGTTGACCGAGAGAAAAGCCCATGGAGAACAAGATCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGc                                                                                                                                                                                                                 <  1:510851/173‑1 (MQ=255)                                                                                 tCAATAAAATAAAGGTTAGGGCATAAACGCCGATTTGCGGTAATTGTTGTGCCAAATCATTCAGTTGACCGAGAGAAAAGCCCATGGAGAACAAGATCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTAAAATAATGAGCAAACCTTCGTACACCGTGAAAATACCTTAGAGTTATTGTTAAGCGCGAAGt                                                                                                                                 <  1:112614/251‑1 (MQ=255)                                                                                    ataaaataaaGGTTAGGGCATAAACGCCGATTTGCGGTAATTGTTGTGCCAAATCATTCAGTTGACCGAGAGAAAAGCCCATGGAGAACAAGATCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTAAAATAATGAGCAAACCTTCGTac                                                                                                                                                                        >  1:139368/1‑209 (MQ=255)                                                                                       aaataaaGGTTAGGGCATAAACGCCGATTTGCGGTAATTGTTGTGCCAAATCATTCAGTTGACCGAGAGAAAAGCCCATGGAGAACAAGATCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTAAaataa                                                                                                                                                                                         <  1:636267/189‑1 (MQ=255)                                                                                       aaataaaGGTTAGGGCATAAACGCCGATTTGCGGTAATTGTTGTGCCAAATCATTCAGTTGACCGAGAGAAAAGCCCATGGAGAACAAGATCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTAAAATAATGATCAAACCTTCGTACACCGTGAAAATACCTTAGAGTTATTGTTAAGCGCGAAGTATAGCa                                                                                                                           >  1:463978/1‑251 (MQ=255)                                                                                       aaataaaGGTTAGGGCATAAACGCCGATTTGCGGTAATTGTTGTGCCAAATCATTCAGTTGACCGAGAGAAAAGCCCATGGAGAACAAGATCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTAAAATAATGAGCAAACCTTCGTacac                                                                                                                                                                      <  1:174742/208‑1 (MQ=255)                                                                                       aaataaaGGTTAGGGCATAAACGCCGATTTGCGGTAATTGTTGTGCCAAATCATTCAGTTGACCGAGAGAAAAGCCCATGGAGAACAAGATCATGTAAAGCAGAACCATGGTTAATCG‑CCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTAAAATAATGAGCAAACCTTCGTACACCGTGAAAATACCTTAGAGTTATTGTTAAGCGCGAAGTATAGCAc                                                                                                                          <  1:436794/251‑1 (MQ=255)                                                                                        aataaaGGTTAGGGCATAAACGCCGATTTGCGTTAATTGTTGTGCCAAATCATTCAGTTGACCGAGAGAAAAGCCCATGGAGAACAAGATCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTAAAATAATGAGCAAACCTTCGTACACCGTGAAAATACCTTAGAGTTATTGTTAAGCGCGAAGTATAGCAc                                                                                                                          <  1:492872/251‑1 (MQ=255)                                                                                        aataaaGGTTAGGGCATAAACGCCGATTTGCGGTAATTGTTGTGCCAAATCATTCAGTTGACCGAGAGAAAAGCCCATGGAGAACAAGATCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTAAAATAATGAGCAAACCTTCGTACACCGTGAAAATACCTTAGAGTTATTGTTAAGCGCGAAGTATAGCAc                                                                                                                          <  1:617342/251‑1 (MQ=255)                                                                                        aataaaGGTTAGGGCATAAACGCCGATTTGCGGTAATTGTTGTGCCAAATCATTCAGTTGACCGAGAGAAAAGCCCATGGAGAACAAGATCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTAAAATAATGAGCAAACCTTCGTACACCGTGAAAATACCTTAGAGTTATTGTTAAGCGCGAAGTATAGCAc                                                                                                                          <  1:564132/251‑1 (MQ=255)                                                                                              ggTTAGGGCATAAACGCCGATTTGTGGTAATTGTTGTGCCAAATCATTCAGTTGACCGAGAGAAAAGCCCATGGAGAACAAGATCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTAAAATAATGAGCAAACCTTCGTACACCGTGAAAATACCTTAGAGTTATTGTTAAGCGCGAAGTATAGCAc                                                                                                                          <  1:76527/245‑1 (MQ=255)                                                                                                ttAGGGCATAAACGCCGATTTGCGGTAATTGTTGTGCCAAATCATTCAGTTGACCGAGAGAAAAGCCCATGGAGAACAAGATCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTAAAGTAATGAGCAAACCTTCGTACACCGTGAAAATACCTTAGAGTTATTGTTAAGCGCGAAGTATAGCACTTTTCTGt                                                                                                                  <  1:75977/251‑1 (MQ=255)                                                                                                   gggCATAAACGCCGATTTGCGGTAATTGTTGTGCCAAATCATTCAGTTGACCGAGAGAAAAGCCCATGGAGAACAAGATCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTAAAATAATGAGCAAACCTTCGTACACCGTGAAAATAc                                                                                                                                                           >  1:457275/1‑207 (MQ=255)                                                                                                      cATAAACGCCGATTTGCGGTAATTGTTGTGCCAAATCATTCAGTTGACCGAGAGAAAAGCCCATGGAGAACAAGATCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTAAAATAATGAGCAAACCTTCGTACACCGTGAAAATACCTTAGAGTTATTGTTAAGCGCGAAGTATAGCACTTTTCTGTCAccc                                                                                                             <  1:682452/250‑1 (MQ=255)                                                                                                      cATAAACGCCGATTTGCGGTAATTGTTGTGCCAAATCATTCAGTTGACCGAGAGAAAAGCCCATGGAGAACAAGATCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTAAAATAATGAGCAAACCTTCGTACACCGTGAAAATACCTTAGAGTTATTGTTAAGCGCGAAGTATAGCACTTTTCTGTCAccc                                                                                                             >  1:84103/1‑250 (MQ=255)                                                                                                                            ttgttgTGCCAAATCATTCAGTTGACCGAGAGAAAAGCCCATGGAGAACAAGATCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTAAAATAATGAGCAAACCTTCGTACACCGTGAAAATACCTTAGAGTTATTg                                                                                                                                              >  1:120521/1‑195 (MQ=255)                                                                                                                            ttgttgTGCCAAATCATTCAGTTGACCGAGAGAAAAGCCCATGGAGAACAAGATCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTAAAATAATGAGCAAACCTTCGTACACCGTGAAAATACCTTAGAGTTATTGTTAAGCGCGAAGTATAGCACTTTTCTGTCACCCCGCCTAATcg                                                                                                   >  1:167715/1‑238 (MQ=255)                                                                                                                                      aaaTCATTCAGTTGACCGAGAGAAAAGCCCATGGAGAACAAGATCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTAAAATAATg                                                                                                                                                                                       >  1:36502/1‑144 (MQ=255)                                                                                                                                       gaTCATTCAGTTGACCGAGAGAAAAGCCCATGGAGAACAAGATCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTAAAATAATGAGCAAACCTTCGTACACCGTGAAAATACCTTAGAGTTATTGTTAAGCGCGAAGTATAGCACTTTTCTGTCACCCCGCCTAATCGCGCGCCGATAAAGCGATTGTTGTa                                                                           >  1:724324/2‑251 (MQ=255)                                                                                                                                        aTCATTCAGTTGACCGAGAGAAAAGCCCATGGAGAACAAGATCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTAAAATAATGAGCAAACCTTCGTACACCGTGaaaa                                                                                                                                                              >  1:635559/1‑167 (MQ=255)                                                                                                                                         tCATTCAGTTGACCGAGAGAAAAGCCCATGGAGAACAAGATCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTAAAATAATGAGCAAACCTTCGTACACCGTGAAAATACCTTagag                                                                                                                                                    <  1:83921/176‑1 (MQ=255)                                                                                                                                          cATTCAGTTGACCGAGAGAAAAGCCCATGGAGAACAAGATCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTAAAATAATGAGCAAACCTTCGTACACCGTGAAAATACCTTAGAGTTATTGTTAAGCGCGAAGTATAGCACTTTTCTGTCACCCCGCCTAATCGCGCGCCGATAAAGCGATTGTTGTATGt                                                                        <  1:640054/251‑1 (MQ=255)                                                                                                                                             tCAGTTGACCGAGAGAAAAGCCCATGGAGAACAAGATCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTAAAATAATGAGCAAACCTTCGTACACCGTGAAAATACCTTAGAGTTATTGTTAAGCGCGAAGTATAGCACTTTTCTGTCACCCCGCCTAAt                                                                                                     >  1:530073/1‑219 (MQ=255)                                                                                                                                                 ttGACCGAGAGAAAAGCCCATGGAGAACAAGATCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTAAAATAATGAGCAAACCTTCGTACACCGTGAAAATACCTTAGAGTTATTGTTAAGCGCGAAGTATAGCACTTTTCTGTCACCCCGCCTAATCGCGCGCCGATAAAGCGATTGTTGTATGTTATaa                                                                   <  1:811930/249‑1 (MQ=255)                                                                                                                                                  tGACCGAGAGAAAAGCCCATGGAGAACAAGATCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCCGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTAAAATAATGAGCAAACCTTCGTACACCGTGAAAATACCTTAGAGTTATTGTTAAGCGCGAAGTATAGCACTTTTCTGTCACCCCGCCTAATCGCGCGCCGATAAAGCGATTGTTGTATGTTATAATGc                                                                <  1:445027/251‑1 (MQ=255)                                                                                                                                                  tGACCGAGAGAAAAGCCCATGGAGAACAAGATCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCCGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTAAAATAATGAGCAAACCTTCGTACACCGTGAAAATACCTTAGAGTTATTGTTAAGCGCGAAGTATAGCACTTTTCTGTCACCCCGCCTAATCGCGCGCCGATAAAGCGATTGTTGTATGTTATAATGc                                                                <  1:443141/251‑1 (MQ=255)                                                                                                                                                      cGAGAGAAAAGCCCATGGAGAACAAGATCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTAAAATAATGAGCAAACCTTCGTACACCGTGAAAATACCTTAGAGTTATTGTTAAGCGCGAAGTATAGCACTTTTCTGTCACCCCGCCTAATCGCGCGCCGATAAAGCGATTGTTGTATGTTATAATGCCAc                                                             >  1:113748/1‑250 (MQ=255)                                                                                                                                                         gagaAAAGCCCATGGAGAACAAGATCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTAAAATAATGAGCAAACCTTCGTACACCGTGAAAATACCTTAGAGTTATTGTTAAGCGCGAAGTATAGCACTTTTCTGTCACCCCGCCTAATCGCGCGCCGATAAAGCGATTGTTGTATGTTATAATGCCACCCGt                                                         >  1:793220/1‑251 (MQ=255)                                                                                                                                                                gCCCATGGAGAACAAGATCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTAAAATAATGAGCAAACCTTCGTACACCGTGAAAATACCTTAGAGTTATTGTTAAGCGCGAGGTATAGCAc                                                                                                                          >  1:448279/1‑179 (MQ=255)                                                                                                                                                                    aTGGAGAACAAGATCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTAAAATAATGAGCAAACCTTCGTACACCGTGAAAATACCTTAGAGTTATTGTTAAGCGCGAAGTATAGCACTTTTCTGTCACCCCGCCTAATCGCGCGCCGATAAAGCGATTGTTGTATGTTATAATGCCACCCGTTTTTTCTTAtt                                              >  1:143995/1‑251 (MQ=255)                                                                                                                                                                       gagaACAAGATCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTAAAATAATGAGCAAACCTTCGTacac                                                                                                                                                                      <  1:218344/128‑1 (MQ=255)                                                                                                                                                                         gaACAAGATCATGTAAAGCAGAACCATTGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTAAAATAATGAGCAAACCTTCGTACACCGTGAAAATACCTTAGAGTTATTGTTAAGCGCGAAGTATAGCACTTTTCTGTCACCCCGCCTAATCGCGCGCCGATAAAGCGATTGTTGTATGt                                                                        <  1:383497/220‑1 (MQ=255)                                                                                                                                                                         gaACAAGATCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTAAAATAATGAGCAAACCTTCGTACACCGTGAAAATACCTTAGAGTTATTGTTAAGCGCGAAGTATAGCACTTTTCTGTCACCCCGCCTAATCGCGCGCCGATAAAGCGATTGTTGTATGTTATaa                                                                   <  1:516480/225‑1 (MQ=255)                                                                                                                                                                         gaACAAGATCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTAAAATAATGAGCAAACCTTCGTACACCGTGAAAATACCTTAGAGTTATTGTTAAGCGCGAAGTATAGCACTTTTCTGTCACCCCGCCTAATCGCGCGCCGATAAAGCGATTGTTGTATGTTATAATGCCACCCGTTTTTTCTTATTGAttt                                         >  1:453031/1‑251 (MQ=255)                                                                                                                                                                              aGATCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAAcc                                                                                                                                                                                                                        <  1:830273/71‑1 (MQ=255)                                                                                                                                                                              aGATCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTAAAATAATGAGCAAACCTTCGTACACCGTGAAAATACCTTAGAGTTATTg                                                                                                                                              <  1:864726/145‑1 (MQ=255)                                                                                                                                                                                aTCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTAAAATAATGAGCAAAcc                                                                                                                                                                               >  1:506068/1‑110 (MQ=255)                                                                                                                                                                                   aTGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTAAAATAATGAGCAAACCTTCGTACACCGTGAAAATACCTTAGAGTTATTGTTAAGCGCGAAGTATAGCACTTTTCTGTCAccc                                                                                                             <  1:854450/173‑1 (MQ=255)                                                                                                                                                                                    tGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTAAAATAATGAGCAAACCTTCGTac                                                                                                                                                                        <  1:230949/113‑1 (MQ=255)                                                                                                                                                                                        aaGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTAAAATAATGAGCAAAcc                                                                                                                                                                               <  1:573833/102‑1 (MQ=255)                                                                                                                                                                                        aaGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTAAAATAATGAGCAAACCTTCGTACACCGTGAAAATACCTTAGAGTTATTGTTAAGCGCGAAGTATAGCAc                                                                                                                          <  1:141408/155‑1 (MQ=255)                                                                                                                                                                                          gCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTAAAATAATGAGCAAACCTTCGTACACCGTGAAAATACCTTAGAGTTATTGTTAAGCGCGAAGTATAGCACTTTTCTGTCACCCCGCCTAATCGCGCGCCGATAAAGCGATTGTTGTATGt                                                                        >  1:866747/1‑203 (MQ=255)                                                                                                                                                                                               aCCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTAACATAATGAGCAAACCTTCGTACACCGTGAAAATACCTTAGAGTTATTGTTAAGCGCGAAGTATAGCACTTTTCTGTCACCCCGCCTAATCGCGCGCCGATAAAGCGATTGTTGTATGTTATAATGCCACCCGTTTTTTCTTATTGATTTTCTCCATGACGTTTCAAATTGt                   >  1:795465/1‑251 (MQ=255)                                                                                                                                                                                               aCCATGGTTAATCGCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTAAAATAATGAGCAAACCTTCGTACACCGTGAAAATACCTTAGAGTTATTGTTAAGCGCGAAGTATAGCACTTTTCTGTCACCCCGCCTAATCGCGCGCCGATAAAGCGATTGTTGTATGTTATAATGCCACCCGTTTTTTCTTATTGATTTTCTCCATGACGTTTCAAATTGt                   >  1:334250/1‑251 (MQ=255)                                                                                                                                                                                                            gCCCCAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTAAAATAATGAGCAAACCTTCGTACACCGTGAAAATACCTTAGAGTTATTGTTAAGCGCGAAGTATAGCACTTTTCTGTCACCCCGCCTAATCGCGCGCCGATAAAGCGATTGTTGTATGTtat                                                                     >  1:171407/1‑188 (MQ=255)                                                                                                                                                                                                                cAATGCGTGCAAAATCGTCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTAAAATAATGAGCAAACCTTCGTACACCGTGAAAATACCTTAGAGTTATTGTTAAGCGCGAAGTATAGCACTTTTCTGTCACCCCGCCTAATCGCGCGCCGATAAAGCGATTGTTGTATGTTATAATGCCACCCGTTTTTTCTTATTGATTTTCTCCATGACGTTTCAAATTGTATTTCAACATCCTGAtt  <  1:224488/251‑1 (MQ=255)                                                                                                                                                                                                                                |                                                                                                                                                                                                                                           CATTTTTTCCGCATTGTGTTTTAGGGGTTGCGGGTGAAGTTTGTCGTAAACCAACAAGCCGATAAAATTAAACCCTTGCGTCAATAAAATAAAGGTTAGGGCATAAACGCCGATTTGCGGTAATTGTTGTGCCAAATCATTCAGTTGACCGAGAGAAAAGCCCATGGAGAACAAGATCATGTAAAGCAGAACCATGGTTAATCGCCCCAATGCGTGCAAAATCATCTGATTTTTCGTTTTAACCATGTAGCCGACAAACATCGGCACTAAAATAATGAGCAAACCTTCGTACACCGTGAAAATACCTTAGAGTTATTGTTAAGCGCGAAGTATAGCACTTTTCTGTCACCCCGCCTAATCGCGCGCCGATAAAGCGATTGTTGTATGTTATAATGCCACCCGTTTTTTCTTATTGATTTTCTCCATGACGTTTCAAATTGTATTTCAACATCCTGATT  >  1/1250619‑1251076

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑