Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,244,905	T→C	intergenic (+105775/+148406)	unknown → / ← unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,244,905	0	T	C	100.0%	153.5 / NA	52	intergenic (+105775/+148406)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/22);  total (30/22)

AAAGCCAATGTCTGCAGCGCATCGCCAGCTAAAATAGCGGTGGCTTCATCAAATGCAATGTGGCAGGTTTGGTGTCCGCGACGCAATTCATCGTCGTCCATAGCGGGCAAATCATCGTGAATTAACGAGTAAGCGTGAATGGCTTCAATAGCGGCGGCGGCGTAATCCAGGTGCGCCATGTCAGCGCCTAGCATTTTGCCGGTGGCGTACACCAAAAAAGGACGAATGCGTTTGCCACCCAATAGTAATCCGTATTTCATGGCTTCAGCCAGTGGCGCGGGGGCGCTGTCAATGTGCGCAAATTGCTTGGCTAAAAATTGATTAATCCGTTGCTGAACGTGCTGTAAATCTTGCGAAAAATGATACATTGCCGACCCGTTATTCTTCCGGTTGATAATCGCTGAGTTTTGCGCTTTCGCTTTTTTGCAACAAAATTTGGATGCGTTGTTCCGCTTGTTGTAAACGTTCCTGACCCAATTGAGCCAATTTG  >  1/1244660‑1245149                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     aaaGCCAATGTCTGCAGCGCATCGCCAGCTAAAATAGCGGTGGCTTCATCAAATGCAATGTGGCAGGTTTGGTGTCCGCGACGCAATTCATCGTCGTCCATAGCGGGCAAATCATCGTGAATTAACGAGTAAGCGTGAATGGCTTCAATAGCGGCGGCGGCGTAATCCAGGTGCGCCATGTCAGCGCCTAGCATTTTGCCGGTGGCGTACACCAAAAAAGGACGAATGCGTTTGCCACCCAATAGCAATcc                                                                                                                                                                                                                                                 <  1:217785/251‑1 (MQ=255)        aTGTCTGCAGCGCATCGCCAGCTAAAATAGCGGTGGCTTCATCAAATGCAATGTGGCAGGTTTGGTGTCCGCGACGCAATTCATCGTCGTCCATAGCGGGCAAATCATCGTGGATTAACGAGTAAGCGTGAATGGCTTCAATAGCGGCGGCGGCGTAATCCAGGTGCGCCATGTCAGCGCCTAGCATTTTGCCGGTGGCGTACACCAAAAAAGGACGAATGCGTTTGCCACCCAATAGCAATCCGTATTTc                                                                                                                                                                                                                                          <  1:786549/251‑1 (MQ=255)        aTGTCTGCAGCGCATCGCCAGCTAAAATAGCGGTGGCTTCATCAAATGCAATGTGGCAGGTTTGGTGTCCGCGACGCAATTCATCGTCGTCCATAGCGGGCAAATCATCGTGAATTAACGAGTAAGCGTGAATGGCTTCAATAGCGGCGGCGGCGTAATCCAGGTGCGCCATGTCAGCGCCTAGCATTTTGCCGGTGGCGTACACCAAAAAAGGACGAATGCGTTTGCCACCCAATAGCAATCCGTATTTc                                                                                                                                                                                                                                          <  1:704380/251‑1 (MQ=255)         tGTCTGCAGCGCATCGCCAGCTAAAATAGCGGTGGCTTCATCAAATGCAATGTGGCAGGTTTGGTGTCCGCGACGCAATTCATCGTCGTCCATAGCGGGCAAATCATCGTGAATTAACGAGTAAGCGTGAATGGCTTCAATAGCGGCGGCGGCGTAATCCAGGTGCGCCATGTCAGCGCCTAGCATTTTGCCGGTGGCGTACACCAAAAAAGGACGAATGCGTTTGCCACCCAATAGCAATCCGTATTTCa                                                                                                                                                                                                                                         >  1:101301/1‑251 (MQ=255)          gTCTGCAGCGCATCGCCAGCTAAAATAGCGGTGGCTTCATCAAATGCAATGTGGCAGGTTTGGTGTCCGCGACGCAATTCATCGTCGTCCATAGCGGGCAAATCATCGTGAATTAACGAGTAAGCGTGAATGGCTTCAATAGCGGCGGCGGCGTAATCCAGGTGCGCCATGTCAGCGCCTAGCATTTTGCCGGTGGCGTACACCAAAAAAGGACGAATGCGTTTGCCACCCAATAGCAATCCGt                                                                                                                                                                                                                                               >  1:786486/1‑244 (MQ=255)                 gcgcATCGCCAGCTAAAATAGCGGTGGCTTCATCAAATGCAATGTGGCAGGTTTGGTGTCCGCGACGCAATTCATCGTCGTCCATAGCGGGCAAATCATCGTGAATTAACGAGTAAGCGTGAATGGCTTCAATAGCGGCGGCGGCGTAATCCAGGTGCGCCATGTCAGCGCCTAGCATTTTGCCGGTGGCGTACACCAAAAAAGGACGAATGCGTTTGCCACCCAATAGCAATc                                                                                                                                                                                                                                                  <  1:745963/234‑1 (MQ=255)                            gCTAAAATAGCGGTGGCTTCATCAAATGCAATGTGGCAGGTTTGGTGTCCGCGACGCAATTCATCGTCGTCCATAGCGGGCAAATCATCGTGAATTAACGAGTAAGCGTGAATGGCTTCAATAGCGGCGGCGGCGTAATCCAGGTGCGCCATGTCAGCGCCTAGCATTTTGCCGGTGGCGTACACCAAAAAAGGACGAATGCGTTTGCCACCCAATAGCAATCCGTATTTCATGGCTTCAGCCAGTGgcg                                                                                                                                                                                                                       >  1:807113/1‑250 (MQ=255)                             cTAAAATAGCGGTGGCTTCATCAAATGCAATGTGGCAGGTTTGGTGTCCGCGACGCAATTCATCGTCGTCCATAGCGGGCAAATCATCGTGAATTAACGAGTAAGCGTGAATGGCTTCAATAGCGGCGGCGGCGTAATCCAGGTGCGCCATGTCAGCGCCTAGCATTTTGCCGGTGGCGTACACCAAAAAAGGACGAATGCGTTTGCCACCCAATAGCAATCCGTATTTCATGGCTTCAGCCAGTGGcgcg                                                                                                                                                                                                                     >  1:262812/1‑251 (MQ=255)                             cTAAAATAGCGGTGGCTTCATCAAATGCAATGTGGCAGGTTTGGTGTCCGCGACGCAATTCATCGTCGTCCATAGCGGGCAAATCATCGTGAATTAACGAGTAAGCGTGAATGGCTTCAATAGCGGCGGCGGCGTAATCCAGGTGCGCCATGTCAGCGCCTAGCATTTTGCCGGTGGCGTACACCAAAAAAGGACGAATGCGTTTGCCACCCAATAGCAATCCGTATTTCATGGCTTCAGCCAGTGGcgcg                                                                                                                                                                                                                     >  1:366546/1‑251 (MQ=255)                                aaaTAGCGGTGGCTTCATCAAATGCAATGTGGCAGGTTTGGTGTCCGCGACGCAATTCATCGTCGTCCATAGCGGGCAAATCATCGTGAATTAACGAGTAAGCGTGAATGGCTTCAATAGCGGCGGCGGCGTAATCCAGGTGCGCCATGTCAGCGCCTAGCATTTTGCCGGTGGCGTACACCAAAAAAGGACGAATGCGTTTGCCACCCAATAGCAATCCGTATTTCATGGCTTCAGCCAGTGGCGCgggg                                                                                                                                                                                                                  >  1:548964/1‑251 (MQ=255)                                 aaTAGCGGTGGCTTCATCAAATGCAATGTGGCAGGTTTGGTGTCCGCGACGCAATTCATCGTCGTCCATAGCGGGCAAATCATCGTGAATTAACGAGTAAGCGTGAATGGCTTCAATAGCGGCGGCGGCGTAATCCAGGTGCGCCATGTCAGCGCCTAGCATTTTGCCGGTGGCGTACACCAAAAAAGGACGAATGCGTTTGCCACCCAATAGCAATCCGTATTTCATGGCTTCAGCCAGTGGCGCggggg                                                                                                                                                                                                                 >  1:180520/1‑251 (MQ=255)                                            cTTCATCAAATGCAATGTGGCAGGTTTGGTGTCCGCGACGCAATTCATCGTCGTCCATAGCGGGCAAATCATCGTGAATTAACGAGTAAGCGTGAATGGCTTCAATAGCGGCGGCGGCGTAATCCAGGTGCGCCATGTCAGCGCCTAGCATTTTGCCGGTGGCGTACACCAAAAAAGGACGAATGCGTTTGCCACCCAATAGCAATCCGTATTTCATGGCTTCAGCCAGTGGCGCGGGGGCGCTGTCAAtg                                                                                                                                                                                                      <  1:244284/251‑1 (MQ=255)                                                 tcaAATGCAATGTGGCAGGTTTGGTGTCCGCGACGCAATTCATCGTCGTCCATAGCGGGCAAATCATCGTGAATTAACGAGTAAGCGTGAATGGCTTCAATAGCGGCGGCGGCGTAATCCAGGTGCGCCATGTCAGCGCCTAGCATTTTGCCGGTGGCGTACACCAAAAAAGGACGAATGCGTTTGCCACCCAATAGCAATCCGTATTTCATGGCTTCAGCCAGTGGCGCGGGGGCGCTGTCAATGTgcgc                                                                                                                                                                                                 <  1:639750/251‑1 (MQ=255)                                                            gtgGCAGGTTTGGTGTCCGCGACGCAATTCATCGTCGTCCATAGCGGGCAAATCATCGTGAATTAACGAGTAAGCGTGAATGGCTTCAATAGCGGCGGCGGCGTAATCCAGGTGCGCCATGTCAGCGCCTAGCATTTTGCCGGTGGCGTACACCAAAAAAGGACGAATGCGTTTGCCACCCAATAGCAATCCGTATTTCATGGCTTCAGCCAGTGGCGCGGGGGCGCTGTCAATGTGCGCAAATTGCTTgg                                                                                                                                                                                      >  1:810382/1‑251 (MQ=255)                                                                  ggTTTGGTGTCCGCGACGCAATTCATCGTCGTCCATAGCGGGCAAATCATCGTGAATTAACGAGTAAGCGTGAATGGCTTCAATAGCGGCGGCGGCGTAATCCAGGTGCGCCATGTCAGCGCCTAGCATTTTGCCGGTGGCGTACACCAAAAAAGGACGAATGCGTTTGCCACCCAATAGCAATCCGTATTTCATGGCTTCAGCCAGTGGCGCGGGGGCGCTGTCAATGTGCGCAAATTGCTTGGCTaaaa                                                                                                                                                                                >  1:319589/1‑251 (MQ=255)                                                                       ggTGTCCGCGACGCAATTCATCGTCGTCCATAGCGGGCAAATCATCGTGAATTAACGAGTAAGCGTGAATGGCTTCAATAGCGGCGGCGGCGTAATCCAGGTGCGCCATGTCAGCGCCTAGCATTTTGCCGGTGGCGTACACCAAAAAAGGACGAATGCGTTTGCCACCCAATAGCAATCCGTATTTCATGGCTTCAGCCAGTGGCGCGGGGGCGCTGTCaa                                                                                                                                                                                                        >  1:36780/1‑222 (MQ=255)                                                                                    cAATTCATCGTCGTCCATAGCGGGCAAATCATCGTGAATTAACGAGTAAGCGTGAATGGCTTCAATAGCGGCGGCGGCGTAATCCAGGTGCGCCATGTCAGCGCCTAGCATTTTGCCGGTGGCGTACACCAAAAAAGGACGAATGCGTTTGCCACCCAATAGCAATCCGTATTTCATGGCTTCAGCCAGTGGCGCGGGGGCGCTGTCAATGTGCGCAAATTGCTTGGCTAAAAATTGATTAATCCGTTGc                                                                                                                                                               <  1:508214/250‑1 (MQ=255)                                                                                      aTTCATCGTCGTCCATAGCGGGCAAATCATCGTGAATTAACGAGTAAGCGTGAATGGCTTCAATAGCGGCGGCGGCGTAATCCAGGTGCGCCATGTCAGCGCCTAGCATTTTGCCGGTGGCGTACACCAAAAAAGGACGAATGCGTTTGCCACCCAATAGCAATCCGTATTTCATGGCTTCAGCCAGTGGCGCGGGGGCGCTGTCAAtg                                                                                                                                                                                                      <  1:566263/209‑1 (MQ=255)                                                                                      aTTCATCGTCGTCCATAGCGGGCAAATCATCGTGAATTAACGAGTAAGCGTGAATGGCTTCAATAGCGGCGGCGGCGTAATCCAGGTGCGCCATGTCAGCGCCTAGCATTTTGCCGGTGGCGTACACCAAAAAAGGACGAATGCGTTTGCCACCCAATAGCAATCCGTATTTCATGGCTTCAGCCAGTGGCGCGGGGGCGCTGTCAATGTGCGCAAATTGCTTGGCTAAAAATTGATTAATCCGTTGCTGa                                                                                                                                                            >  1:783577/1‑251 (MQ=255)                                                                                          aTCGTCGTCCATAGCGGGCAAATCATCGTGAATTAACGAGTAAGCGTGAATGGCTTCAATAGCGGCGGCGGCGTAATCCAGGTGCGCCATGTCAGCGCCTAGCATTTTGCCGGCGGCGTACACCAAAAAAGGACGAATGCGTTTGCCACCCAATAGCAATCCGTATTTCATGGCTTCAGCCAGTGGCGCGGGGGCGCTGTCAATGTGCGCAAATTGCTTGGCTAAAAATTGATTAATCCGTTGCTGAACGt                                                                                                                                                        >  1:345992/1‑251 (MQ=255)                                                                                               cgtcCATAGCGGGCAAATCATCGTGAATTAACGAGTAAGCGTGAATGGCTTCAATAGCGGCGGCGGCGTAATCCAGGTGCGCCATGTCAGCGCCTAGCATTTTGCCGGTGGCGTACACCAAAAAAGGACGAATGCGTTTGCCACCCAATAGCAATCCGTATTTCATGGCTTCAGCCAGTGGCGCGGGGGCGCTGTCAATGTGCGCAAATTGCTTGGCTAAAAATTGATTAATCCGTTGCTGAACGTg                                                                                                                                                       >  1:315088/1‑247 (MQ=255)                                                                                                                    cGTGAATTAACGAGTAAGCGTGAATGGCTTCAATAGCGGCGGCGGCGTAATCCAGGTGCGCCATGTCAGCGCCTAGCATTTTGCCGGTGGCGTACACCAAAAAAGGACGAATGCGTTTGCCACCCAATAGCAATCCGTATTTCATGGCTTCAGCCAGTGGCGCGGGGGCGCTGTCAATGTGCGCAAATTGCTTGGCTAAAAATTGATTAATCCGTTGCTGAACGTGCTGTAAATCTTGCGAAAAATGATAc                                                                                                                              <  1:856077/251‑1 (MQ=255)                                                                                                                    cGTGAATTAACGAGTAAGCGTGAATGGCTTCAATAGCGGCGGCGGCGTAATCCAGGTGCGCCATGTCAGCGCCTAGCATTTTGCCGGTGGCGTACACCAAAAAAGGACGAATGCGTTTGCCACCCAATAGCAATCCGTATTTCATGGCTTCAGCCAGTGGCGCGGGGGCGCTGTCAATGTGCGCAAATTGCTTGGCTAAAAATTGATTAATCCGTTGCTGAACGTGCTGTAAATCTTGCGAAAAATGATAc                                                                                                                              <  1:371629/251‑1 (MQ=255)                                                                                                                                    aGCGTGAATGGCTTCAATAGCGGCGGCGGCGTAATCCAGGTGCGCCATGTCAGCGCCTAGCATTTTGCCGGTGGCGTACACCAAAAAAGGACGAATGCGTTTGCCACCCAATAGCAATCCGTATTTCATGGCTTCAGCCAGTGGCGCGGGGGCGCTGTCAATGTGCGCAAATTGCTTGGCTAAAAATTGATTAATCCGTTGCTGAACGTGCTGTAAATCTTGCGAAAAATGATACATTGCCGACCCGTTAt                                                                                                              >  1:653770/1‑251 (MQ=255)                                                                                                                                     gCGTGAATGGCTTCAATAGCGGCGGCGGCGTAATCCAGGTGCGCCATGTCAGCGCCTAGCATTTTGCCGGTGGCGTACACCAAAAAAGGACGAATGCGTTTGCCACCCAATAGCAATCCGTATTTCATGGCTTCAGCCAGTGGCGCGGGGGCGCTGTCAATGTGCGCAAATTGCTTGGCTAAAAATTGATTAATCCGTTGCTGAACGTGCTGTAAATCTTGCGAAAAATGATACATTGCCGACCCGTTAtt                                                                                                             >  1:52388/1‑251 (MQ=255)                                                                                                                                      cGTGAATGGCTTCAATAGCGGCGGCGGCGTAATCCAGGTGCGCCATGTCAGCGCCTAGCATTTTGCCGGTGGCGTACACCAAAAAAGGACGAATGCGTTTGCCACCCAATAGCAATCCGTATTTCATGGCTTCAGCCAGTGGCGCGGGGGCGCTGTCAATGTGCGCAAATTGCTTGGCTAAAAATTGATTAATCCGTTGCTGAAc                                                                                                                                                          >  1:432903/1‑205 (MQ=255)                                                                                                                                       gTGAATGGCTTCAATAGCGGCGGCGGCGTAATCCAGGTGCGCCATGTCAGCGCCTAGCATTTTGCCGGTGGCGTACACCAAAAAAGGACGAATGCGTTTGCCACCCAATAGCAATCCGTATTTCATGGCTTCAGCCAGTGGCGCGGGGGCGCTGTCAATGTGCGCAAATTGCTTGGCTAAAAATTGATTAATCCGTTGCTGAACGTGCTGTAAATCTTGCGAAAAATGATAc                                                                                                                              <  1:248367/232‑1 (MQ=255)                                                                                                                                             ggCTTCAATAGCGGCGGCGGCGTAATCCAGGTGCGCCATGTCAGCGCCTAGCATTTTGCCGGTGGCGTACACCAAAAAAGGACGAATGCGTTTGCCACCCAATAGCAATCCGTATTTCATGGCTTCAGCCAGTGGCGCGGGGGCGCTGTCAATGTGCGCAAATTGCTTGGCTAAAAATTGAtt                                                                                                                                                                         >  1:105228/1‑183 (MQ=255)                                                                                                                                              gCTTCAATAGCGGCGGCGGCGTAATCCAGGTGCGCCATGTCAGCGCCTAGCATTTTGCCGGTGGCGTACACCAAAAAAGGACGAATGCGTTTGCCACCCAATAGCAATCCGTATTTCATGGCTTCAGCCAGTGGCGCGGGGGCGCTGTCAATGTGCGCAAATTGCTTGGCTAAAAATTGATTAATCCGTTGCTGAACGTGCTGTAAATCTTg                                                                                                                                           <  1:244659/212‑1 (MQ=255)                                                                                                                                               cTTCAATAGCGGCGGCGGCGTAATCCAGGTGCGCCATGTCAGCGCCTAGCATTTTGCCGGTGGCGTACACCAAAAAAGGACGAATGCGTTTGCCACCCAATAGCAATCCGTATTTCATGGCTTCAGCCAGTGGCGCGGGGGCGCTGTCAATGTGCGCAAATTGCTTGGCTAAAAATTGATTAATCCGTTGCTGAACGTGCTGTAAATCTTGCGAAAAATGATACATTGCCGACCCGTTAttcttc                                                                                                         >  1:853410/1‑245 (MQ=255)                                                                                                                                                  cAATAGCGGCGGCGGCGTAATCCAGGTGCGCCATGTCAGCGCCTAGCATTTTGCCGGTGGCGTACACCAAAAAAGGACGAATGCGTTTGCCACCCAATAGCAATCCGTATTTCATGGCTTCAGCCAGTGGCGCGGGGGCGCTGTCAATGTGCGCAAATTGCTTGGCTAAAAATTGATTAATCCGTTGCTGAACGTGCTGTAAATCTTGCGAAAAATGATACATTGCCGACCCGTTATTCTTCCGGTTGATa                                                                                                >  1:851300/1‑251 (MQ=255)                                                                                                                                                        cggcggcggcgTAATCCAGGTGCGCCATGTCAGCGCCTAGCATTTTGCCGGTGGCGTACACCAAAAAAGGACGAATGCGTTTGCCACCCAATAGCAATCCGTATTTCATGGCTTCAGCCAGTGGCGCGGGGGCGCTGTCAATGTGCGCAAATTGCTTGGCTAAAAATTGATTAATCCGTTGCTGAACGTGCTGTAAATCTTGCGAAAAATGATACATTGCCGACCCGTTATTCTTCCGGTTGATAATCGc                                                                                           <  1:178789/250‑1 (MQ=255)                                                                                                                                                                gcgTAATCCAGGTGCGCCATGTCAGCGCCTAGCATTTTGCCGGTGGCGTACACCAAAAAAGGACGAATGCGTTTGCCACCCAATAGCAATCCGTATTTCATGGCTTCAGCCAGTGGCGCGGGGGCGCTGTCAATGTGCGCAAATTGCTTGGCTAAAAATTGATTAATcc                                                                                                                                                                    >  1:799849/1‑169 (MQ=255)                                                                                                                                                                   tAATCCAGGTGCGCCATGTCAGCGCCTAGCATTTTGCCGGTGGCGTACACCAAAAAAGGACGAATGCGTTTGCCACCCAATAGCAATCCGTATTTCATGGCTTCAGCCAGTGGCGCGGGGGCGCTGTCAATGTGCGCAAATTGCTTGGCTAAAAATTGATTAATCCGTTGCTGAACGTGCTGTAAATCTTGCGAAAAAt                                                                                                                                   <  1:421934/199‑1 (MQ=255)                                                                                                                                                                   tAATCCAGGTGCGCCATGTCAGCGCCTAGCATTTTGCCGGTGGCGTACACCAAAAAAGGACGAATGCGTTTGCCACCCAATAGCAATCCGTATTTCATGGCTTCAGCCAGTGGCGCGGGGGCGCTGTCAATGTGCGCAAATTGCTTGGCTAAAAATTGATTAATCCGTTGCTGAACGTGCTGTAAATCTTGCGAAAAATGATACATTGCCGACCCGTTATTCTTCCGGTTGATAATCGCTGAGTTTTgcg                                                                                <  1:763615/250‑1 (MQ=255)                                                                                                                                                                     aTCCAGGTGCGCCATGTCAGCGCCTAGCATTTTGCCGGTGGCGTACACCAAAAAAGGACGAATGCGTTTGCCACCCAATAGCAATCCGTATTTCATGGCTTCAGCCAGTGGCGCGGGGGCGCTGTCAATGTGCGCAAATTGCTTGGCTAAAAATTGATTAATCCGTTGCTGAACGTGCTGTAAATCTTGCGAAAAATGATACATTGCCGACCCGTTATTCTTCCGGTTGATAATCGCTGAGTTTTGcgctt                                                                             >  1:551444/1‑251 (MQ=255)                                                                                                                                                                     aTCCAGGTGCGCCATGTCAGCGCCTAGCATTTTGCCGGTGGCGTACACCAAAAAAGGACGAATGCGTTTGCCACCCAATAGCAATCCGTATTTCATGGCTTCAGCCAGTGGCGCGGGGGCGCTGTCAATGTGCGCAAATTGCTTGGCTAAAAATTGATTAATCCGTTGCTGAACGTGCTGTAAATCTTGCGAAAAATGATACATTGCCGACCCGTTATTCTTCCGGTTGATAATCGCTGAGTTTTGcgctt                                                                             >  1:664439/1‑251 (MQ=255)                                                                                                                                                                             gcgcCATGTCAGCGCCTAGCATTTTGCCGGTGGCGTACACCAAAAAAGGACGAATGCGTTTGCCACCCAATAGCAATCCGTATTTCATGGCt                                                                                                                                                                                                                                    <  1:656410/92‑1 (MQ=255)                                                                                                                                                                                ccATGTCAGCGCCTAGCATTTTGCCGGTGGCGTACACCAAAAAAGGACGAATGCGTTTGCCACCCAATAGCAATCCGTATTTCATGGCTTCAGCCAGTGGCGCGGGGGCGCTGTCAATGTGCGCAAATTGCTTGGCTAAAAATTGATTAATCCGTTGCTGAACGTGCTGTAAATCTTGCGAAAAATGATACATTGCCGACCCGTTATTCTTCCGGTTGATAATCGCTGAGTTTTGCGCTTTCGCTTTTTTg                                                                  >  1:613524/1‑251 (MQ=255)                                                                                                                                                                                           ccTAGCATTTTGCCGGTGGCGTACACCAAAAAAGGACGAATGCGTTTGCCACCCAATAGCAATCCGTATTTCATGGCTTCAGCCAGTGGCGCGGGGGCGCTGTCAATGTGCGCAAATTGCTTGGCTAAAAATTGATTAATCCGTTGCTGAACGTGCTGTAAATCTTGCGAAAAATGATACATTGCCGACCCGTTATTCTTCCGGTTGATAATCGCTGAGTTTTGCGCTTTCGCTTTTTTGCAACAAAAttt                                                       <  1:336152/251‑1 (MQ=255)                                                                                                                                                                                             tAGCATTTTGCCGGTGGCGTACACCAAAAAAGGACGAATGCGTTTGCCACCCAATAGCAATCCGTATTTCATGGCTTCAGCCAGTGGCGCGGGGGCGCTGTCAATGTGCGCAAATTGCTTGGCTAAAAATTGATTAATCCGTTGCTGAACGTGCTGTAAATCTTGCGAAAAATGATACATTGCCGACCCGTTATTCTTCCGGTTGATAATCGCTGAGTTTTGCGCTTTCGCTTTTTTGCAACAAAATTTgg                                                     >  1:629377/1‑251 (MQ=255)                                                                                                                                                                                                cATTTTGCCGGTGGCGTACACCAAAAAAGGACGAATGCGTTTGCCACCCAATAGCAATCCGTATTTCATGGCTTCAGCCAGTGGCGCGGGGGCGCTGTCAATGTGCGCAAATTGCTTGGCTAAAAATTGATTAATCCGTTGCTGAACGTGCTGTAAATCTTGCGAAAAATGATACATTGCCGACCCGTTATTCTTCCGGTTGATAATCGCTGAGTTTTGCGCTTTCGCTTTTTTGCAACAAAATTTGGa                                                    <  1:764922/249‑1 (MQ=255)                                                                                                                                                                                                        cGGTGGCGTACACCAAAAAAGGACGAATGCGTTTGCCACCCAATAGCAATCCGTATTTCATGGCTTCAGCCAGTGGCGCGGGGGCGCTGTCAATGTGCGCAAATTGCTTGGCTAAAAATTGATTAATCCGTTGCTGAACGTGCTGTAAATCTTGCGAAAAATGATACATTGCCGACCCGTTATTCTTCCGGTTGATAATCGCTGAGTTTTGCGCTTTCGCTTTTTTGCAACAAAATTTGGATGCGTTGTTc                                          <  1:505500/251‑1 (MQ=255)                                                                                                                                                                                                                  cacCAAAAAAGGACGAATGCGTTTGCCACCCAATAGCAATCCGTATTTCATGGCTTCAGCCAGTGGCGCGGGGGCGCTGTCAATGTGCGCAAATTGCTTGGCTAAAAATTGATTAATCCGTTGCTGAACGTGCTGTAAATCTTGCGAAAAATGATACATTGc                                                                                                                         >  1:793121/1‑162 (MQ=255)                                                                                                                                                                                                                    ccAAAAAAGGACGAATGCGTTTGCCACCCAATAGCAATCCGTATTTCATGGCTTCAGCCAGTGGCGCGGGGGCGCTGTCAATGTGCGCAAATTGCTTGGCTAAAAATTGATTAATCCGTTGCTGAACGTGCTGTAAATCTTGCGAAAAATGATACATTGCCGACCCGTTATTCTTCCGGTTGATAATCGCTGAGTTTTGCGCTTTCGCTTTTTTGCAACAAAATTTGGATGCGTTGTTCCGCTTGTTGTaa                              >  1:550069/1‑251 (MQ=255)                                                                                                                                                                                                                             gACGAATGCGTTTGCCACCCAATAGCAATCCGTATTTCATGGCTTCAGCCAGTGGCGCGGGGGCGCTGTCAATGTGCGCAAATTGCTTGGCTAAAAATTGATTAATCCGTTGCTGAACGTGCTGTAAATCTTGCGAAAAATGATACATTGCCGACCCGTTATTCTTCCGAGTGATAATCGCTGAGTTTTGcgctttcgc                                                                         >  1:431806/1‑199 (MQ=255)                                                                                                                                                                                                                                 aaTGCGTTTGCCACCCAATAGCAATCCGTATTTCATGGCTTCAGCCAGTGGCGCGGGGGCGCTGTCAATGTGCGCAAATTGCTTGGCTAAAAATTGATTAATCCGTTGCTGAACGTGCTGTAAATCTTGCGAAAAATGATACATTGCCGACCCGTTATTCTTCCGGTTGATAATCGCTGAGTTTTGCGCTTTCGCTTTTTTGCAACAAAATTTGGATGCGTTGTTCCGCTTGTTGTAAACGTTCCTGAcc                  <  1:9613/250‑1 (MQ=255)                                                                                                                                                                                                                                 aaTGCGTTTGCCACCCAATAGCAATCCGTATTTCATGGCTTCAGCCAGTGGCGCGGGGGCGCTGTCAATGTGCGCAAATTGCTTGGCTAAAAATTGATTAATCCGTTGCTGAACGTGCTGTAAATCTTGCGAAAAATGATACATTGCCGACCCGTTATTCTTCCGGTTGATAATCGCTGAGTTTTGCGCTTTCGCTTTTTTGCAACAAAATTTGGATGCGTTGTTCCGCTTGTTGTAAACGTTCCTGAcc                  <  1:62655/250‑1 (MQ=255)                                                                                                                                                                                                                                              cccAATAGCAATCCGTATTTCATGGCTTCAGCCAGTGGCGCGGGGGCGCTGTCAATGTGCGCAAATTGCTTGGCTAAAAATTGATTAATCCGTTGCTGAACGTGCTGTaa                                                                                                                                                 >  1:125954/1‑110 (MQ=255)                                                                                                                                                                                                                                              cccAATAGCAATCCGTATTTCATGGCTTCAGCCAGTGGCGCGGGGGCGCTGTCAATGTGCGCAAATTGCTTGGCTAAAAATTGATTAATCCGTTGCTGAACGTGCTGTAAATCTTGCGAAAAATGATACATTGCCGACCCGTTATTCTTCCGGTTGATAATCGCTGAGTTTTGCGCTTTCGCTTTTTTGCAACAAAATTTGGATGCGTTGTTCCGCTTGTTGTAAACGTTCCTGACCCAATTGAGCCAAtt    >  1:556905/1‑251 (MQ=255)                                                                                                                                                                                                                                                cAATAGCAATCCGTATTTCATGGCTTCAGCCAGTGGCGCGGGGGCGCTGTCAATGTGCGCAAATTGCTTGGCTAAAAATTGAtt                                                                                                                                                                         >  1:575427/1‑84 (MQ=255)                                                                                                                                                                                                                                                cAATAGCAATCCGTATTTCATGGCTTCAGCCAGTGGCGCGGGGGCGCTGTCAATGTGCGCAAATTGCTTGGCTAAAAATTGATTAATCCGTTGCTGAACGTGCTGTAAATCTTGCGAAAAATGATACATTGCCGACCCGTTATTCTTCCGGTTGATAATCGCTGAGTTTTGCGCTTTCGCTTTTTTGCAACAAAATTTGGATGCGTTGTTCCGCTTGTTGTAAACGTTCCTGACCCAATTGAGCCAATTTg  <  1:808432/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     AAAGCCAATGTCTGCAGCGCATCGCCAGCTAAAATAGCGGTGGCTTCATCAAATGCAATGTGGCAGGTTTGGTGTCCGCGACGCAATTCATCGTCGTCCATAGCGGGCAAATCATCGTGAATTAACGAGTAAGCGTGAATGGCTTCAATAGCGGCGGCGGCGTAATCCAGGTGCGCCATGTCAGCGCCTAGCATTTTGCCGGTGGCGTACACCAAAAAAGGACGAATGCGTTTGCCACCCAATAGTAATCCGTATTTCATGGCTTCAGCCAGTGGCGCGGGGGCGCTGTCAATGTGCGCAAATTGCTTGGCTAAAAATTGATTAATCCGTTGCTGAACGTGCTGTAAATCTTGCGAAAAATGATACATTGCCGACCCGTTATTCTTCCGGTTGATAATCGCTGAGTTTTGCGCTTTCGCTTTTTTGCAACAAAATTTGGATGCGTTGTTCCGCTTGTTGTAAACGTTCCTGACCCAATTGAGCCAATTTG  >  1/1244660‑1245149

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑