Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,239,132	A→G	intergenic (+100002/+154179)	unknown → / ← unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,239,132	0	A	G	100.0%	204.8 / NA	71	intergenic (+100002/+154179)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/45);  total (26/45)

GATTATTATTCTTTCGTTGTTGCTCGGCATGAAAGCCGGCGATTCCTTTAAGGCCGGATTACAAATCGGTATTGGCTTTGTAGGGATCGGGCTGGTTATCGGCTTAATGCTGGATTCTATCGGACCGGCAGCAAAAGCGATGGCGCAGGCATTTGATATTAATTTGAATGTGGTGGATATCGGCTGGCCGGGCGCCGCACCGATTACTTGGGCATCGCAAATTGCGTTAATCGCTATTCCTATCACAATCGCGGTGAATATCGTCATGTTGCTGTTGAAACTGACCCGCGTGGTGAACGTAGATATTTGGAATATCTGGCATATGACCTTCACCGGCGCCATTGTGCATATCGCCACCGGTTCTTATTGGCTGGGTATTCTCGGGGTTGTGATTCACGCTGCGTTTGTTTACAAACTGGGAGATTGGTTTAGTCGCGATACGAAAAATTTCTTTGAACTGGACGGCATTGCCATTCCGCAC  >  1/1238888‑1239368                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                             gATTATTATTCTTTCGTTGTTGCTCGGCATGAAAGCCGGCGATTCCTTTAAGGCCGGATTACAAATCGGTATTGGCTTTGTAGGGATCGGGCTGGTTATCGGCTTAATGCTGGATTCTATCGGACCGGCAGCAAAAGCGATGGTGCAGGCATTTGATATTAATTTGAATGTGGTGGATATCGGCTGGCCGGGCGCCGCACCGATTACTTGGGCATCGCAAATTGCGTTAATCGCTATTCCTATCGCAATcg                                                                                                                                                                                                                                        >  1:261921/1‑251 (MQ=255)                                aaaGCCGGCGATTCCTTTAAGGCCGGATTACAAATCGGTATTGGCTTTGTAGGGATCGGGCTGGTTATCGGCTTAATGCTGGATTCTATCGGACCGGCAGCAAAAGCGATGGTGCAGGCATTTGATATTAATTTGAATGTGGTGGATATCGGCTGGCCGGGCGCCGCACCGATTACTTGGGCATCGCAAATTGCGTTAATCGCTATTCCTATCGCAATCGCGGTGAATATCGTCATGTTGCTGTTGAAACt                                                                                                                                                                                                         <  1:591527/251‑1 (MQ=255)                                             ccTTTAAGGCCGGATTACAAATCGGTATTGGCTTTGTAGGGATCGGGCTGGTTATCGGCTTAATGCTGGATTCTATCGGACCGGCAGCAAAAGCGATGGTGCAGGCATTTGATATTAATTTGAATGTGGTGGATATCGGCTGGCCGGGCGCCGCACCGATTACTTGGGCATCGCAAATTGCGTTAATCGCTATTCCTATCGCAATCGCGGTGAATATCGTCATGTTGCTGTTGAAACTGACCCGCgtggtg                                                                                                                                                                                            >  1:394039/1‑251 (MQ=255)                                                  aaGGCCGGATTACAAATCGGTATTGGCTTTGTAGGGATCGGGCTGGTTATCGGCTTAATGCTGGATTCTATCGGACCGGCAGCAAAAGCGATGGTGCAGGCATTTGATATTAATTTGAATGTGGTGGATATCGGCTGGCCGGGCGCCGCACCGATTACTTGGGCATCGCAAATTGCGTTAATCGCTATTCCTATCGCAATCGCGGTGAATATCGTCATGTTGCTGTTGAAACTGACCCGCGTGGTGAACGt                                                                                                                                                                                       <  1:135978/251‑1 (MQ=255)                                                      ccGGATTACAAATCGGTATTGGCTTTGTAGGGATCGGGCTGGTTATCGGCTTAATGCTGGATTCTATCGGACCGGCAGCAAAAGCGATGGTGCAGGCATTTGATATTAATTTGAATGTGGTGGATATCGGCTGGCCGGGCGCCGCACCGATTACTTGGGCATCGCAAATTGCGTTAATCGCTATTCCTATCGCAATCGCGGTGAATATCGTCATGTTGCTGTTGAAACTGACCCGCGTGGTGAACGTAGa                                                                                                                                                                                    <  1:870080/250‑1 (MQ=255)                                                       cGGATTACAAATCGGTATTGGCTTTGTAGGGATCGGGCTGGTTATCGGCTTAATGCTGGATTCTATCGGACCGGCAGCAAAAGCGATGGTGCAGGCATTTGATATTAATTTGAATGTGGTGGATATCGGCTGGCCGGGCGCCGCACCGATTACTTGGGCATCGCAAATTGCGTTAATCGCTATTCCTATCGCAATCGCGGTGAATATCGTCATGTTGCTGTTGAAACTGACCCGCGTGGTGAACGTAGata                                                                                                                                                                                  <  1:256689/251‑1 (MQ=255)                                                         gATTACAAATCGGTATTGGCTTTGTAGGGATCGGGCTGGTTATCGGCTTAATGCTGGATTCTATCGGACCGGCAGCAAAAGCGATGGTGCAGGCATTTGATATTAATTTGAATGTGGTGGATATCGGCTGGCCGGGCGCCGCACCGATTACTTGGGCATCGCAAATTGCGTTAATCGCTATTCCTATCGCAATCGCGGTGAATATCGTCATGATGCTGTTGAAACTGACCCGCGTGGTGAACGTAGATAtt                                                                                                                                                                                >  1:874286/1‑251 (MQ=255)                                                                 aTCGGTATTGGCTTTGTAGGGATCGGGCTGGTTATCGGCTTAATGCTGGATTCTATCGGACCGGCAGCAAAAGCGATGGTGCAGGCATTTGATATTAATTTGAATGTGGTGGATATCGGCTGGCCGGGCGCCGCACCGATTACTTGGGCATCGCAAATTGCGTTAATCGCTATTCCTATCGCAATCGCGGTGAATATCGTCATGTTGCTGTTGAAACTGACCCGCGTGGTGAACGTAGATATTTGGAatat                                                                                                                                                                        <  1:248461/251‑1 (MQ=255)                                                                     gTATTGGCTTTGTAGGGATCGGGCTGGTTATCGGCTTAATGCTGGATTCTATCGGACCGGCAGCAAAAGCGATGGTGCAGGCATTTGATATTAATTTGAATGTGGTGGATATCGGCTGGCCGGGCGCCGCACCGATTACTTGGGCATCGCAAATTGCGTTAATCGCTATTCCTATCGCAATCGCGGTGAATATCGt                                                                                                                                                                                                                           <  1:639247/196‑1 (MQ=255)                                                                           gCTTTGTAGGGATCGGGCTGGTTATCGGCTTAATGCTGGATTCTATCGGACCGGCAGCAAAAGCGATGGTGCAGGCATTTGATATTAATTTGAATGTGGTGGATATCGGCTGGCCGGGCGCCGCACCGATTACTTGGGCATCGCAAATTGCGTTAATCGCTATTCCTATCGCAATCGCGGTGAATATCGTCATGTTGCTGTTGAAACTg                                                                                                                                                                                                        >  1:238537/1‑209 (MQ=255)                                                                           gCTTTGTAGGGATCGGGCTGGTTATCGGCTTAATGCTGGATTCTATCGGACCGGCAGCAAAAGCGATGGTGCAGGCATTTGATATTAATTTGAATGTGGTGGATATCGGCTGGCCGGGCGCCGCACCGATTACTTGGGCATCGCAAATTGCGTTAATCGCTATTCCTATCGCAATCGCGGTGAATATCGTCATGTTGCTGTTGAAACTGACCCGCGTGGTGAACGTAg                                                                                                                                                                                     <  1:491711/228‑1 (MQ=255)                                                                           gCTTTGTAGGGATCGGGCTGGTTATCGGCTTAATGCTGGATTCTATCGGACCGGCAGCAAAAGCGATGGTGCAGGCATTTGATATTAATTTGAATGTGGTGGATATCGGCTGGCCGGGCGCCGCACCGATTACTTGGGCATCGCAAATTGCGTTAATCGCTATTCCTATCGCAATCGCGGTGAATATCGTCATGTTGCTGTTGAAACTGACCCGCGTGGTGAACGTAGATATTTGGAATATCTGGCATATg                                                                                                                                                              >  1:506700/1‑251 (MQ=255)                                                                             tttGTAGGGATCGGGCTGGTTATCGGCTTAATGCTGGATTCTATCGGACCGGCAGCAAAAGCGATGGTGCAGGCATTTGATATTAATTTGAATGTGGTGGATATCGGCTGGCCGGGCGCCGCACCGATTACTTGGGCATCGCAAATTGCGTTAATCGCTATTCCTATCGCAATCGCGGTGAATATCGTCATGTTGCTGTTGAAACTGACCCGCGTGGTGAACGTAGATATTTGGAATATCTGGCATATGAc                                                                                                                                                            <  1:568520/251‑1 (MQ=255)                                                                                gTAGGGATCGGGCTGGTTATCGGCTTAATGCTGGATTCTATCGGACCGGCAGCAAAAGCGATGGTGCAGGCATTTGATATTAATTTGAATGTGGTGGATATCGGCTGGCCGGGCGCCGCACCGATTACTTGGGCATCGCAAATTGCGTTAATCGCTATTCCTATCGCAATCGCGGTGAATATCGTCATGTTGc                                                                                                                                                                                                                   >  1:353157/1‑193 (MQ=255)                                                                                     gATCGGGCTGGTTATCGGCTTAATGCTGGATTCTATCGGACCGGCAGCAAAAGCGATGGTGCAGGCATTTGATATTAATTTGAATGTGGTGGATATCGGCTGGCCGGGCGCCGCACCGATTACTTGGGCATCGCAAATTGCGTTAATCGCTATTCCTATCGCAATCGCGGTGAATATCGTCATGTTGCTGTTGAAACTGACCCGCGTGGTGAACGTAGATATTTGGAATATCTGGCATATGACCTTCAcc                                                                                                                                                     <  1:493919/250‑1 (MQ=255)                                                                                     gATCGGGCTGGTTATCGGCTTAATGCTGGATTCTATCGGACCGGCAGCAAAAGCGATGGTGCAGGCATTTGATATTAATTTGAATGTGGTGGATATCGGCTGGCCGGGCGCCGCACCGATTACTTGGGCATCGCAAATTGCGTTAATCGCTATTCCTATCGCAATCGCGGTGAATATCGTCATGTTGCTGTTGAAACTGACCCGCGTGGTGAACGTAGATATTTGGAATATCTGGCATATGACCTTCACCg                                                                                                                                                    >  1:534292/1‑251 (MQ=255)                                                                                               gTTATCGGCTTAATGCTGGATTCTATCGGACCGGCAGCAAAAGCGATGGTGCAGGCATTTGATATTAATTTGAATGTGGTGGATATCGGCTGGCCGGGCGCCGCACCGATTACTTGGGCATCGCAAATTGCGTTAATCGCTATTCCTATCGCAATCGCGGTGAATATCGTCATGTTGCTGTTGAAACTGACCCGCGTGGTGAACGTAGATATTTGGAATATCTGGCATATGACCTTCACCGGCGCCATtg                                                                                                                                           <  1:360938/250‑1 (MQ=255)                                                                                                 tATCGGCTTAATGCTGGATTCTATCGGACCGGCAGCAAAAGCGATGGTGCAGGCATTTGATATTAATTTGAATGTGGTGGATATCGGCTGGCCGGGCGCCGCACCGATTACTTGGGCATCGCAAATTGCGTTAATCGCTATTCCTATCGCAATCGCGGTGAATATCGTCATGTTGCTGTTGAAACTGACCCGCGTGGTGAACGTAGATATTTGGAATATCTGGCATATGACCTTCACCGGCGCCATTGTGc                                                                                                                                        <  1:651414/251‑1 (MQ=255)                                                                                                 tATCGGCTTAATGCTGGATTCTATCGGACCGGCAGCAAAAGCGATGGTGCAGGCATTTGATATTAATTTGAATGTGGTGGATATCGGCTGGCCGGGCGCCGCACCGATTACTTGGGCATCGCAAATTGCGTTAATCGCTATTCCTATCGCAATCGCGGTGAATATCGTCATGTTGCTGTTGAAACTGACCCGCGTGGTGAACGTAGATATTTGGAATATCTGGCATATGACCTTCACCGGCGCCATTGTGc                                                                                                                                        <  1:10770/251‑1 (MQ=255)                                                                                                   tCGGCTTAATGCTGGATTCTATCGGACCGGCAGCAAAAGCGATGGTGCAGGCATTTGATATTAATTTGAATGTGGTGGATATCGGCTGGCCGGGCGCCGCACCGATTACTTGGGCATCGCAAATTGCGTTAATCGCTATTCCTATCGCAATCGCGGTGAATATCGTCATGTTGCTGTTGAAACTGACCCGCGTGGTGAACGTAGATATTTGGAATATCTGGCATATGACCTTCACCGGCGCCATTGTGCat                                                                                                                                      <  1:246123/251‑1 (MQ=255)                                                                                                   tCGGCTTAATGCTGGATTCTATCGGACCGGCAGCAAAAGCGATGGTGCAGGCATTTGATATTAATTTGAATGTGGTGGATATCGGCTGGCCGGGCGCCGCACCGATTACTTGGGCATCGCAAATTGCGTTAATCGCTATTCCTATCGCAATCGCGGTGAATATCGTCATGTTGCTGTTGAAACTGACCCGCGTGGTGAACGTAGATATTTGGAATATCTGGCATATGACCTTCACCGGCGCCATTGTGCat                                                                                                                                      <  1:513687/251‑1 (MQ=255)                                                                                                      gCTTAATGCTGGATTCTATCGGACCGGCAGCAAAAGCGATGGTGCAGGCATTTGATATTAATTTGAATGTGGTGGATATCGGCTGGCCGGGCGCCGCACCGATTACTTGGGCATCGCAAATTGCGTTAATCGCTATTCCTATCGCAATCGCGGTGAATATCGTCATGTTGCTGTTGAAACTGACCCGCGTGGTGAACGTAGATATTTGGAATATCTGGCATATGACCTTCACCGGCGCCATTGTGCatat                                                                                                                                    >  1:781884/1‑250 (MQ=255)                                                                                                           aTGCTGGATTCTATCGGACCGGCAGCAAAAGCGATGGTGCAGGCATTTGATATTAATTTGAATGTGGTGGATATCGGCTGGCCGGGCGCCGCACCGATTACTTGGGCATCGCAAATTGCGTTAATCGCTATTCCTATCGCAATCGCGGTGAATATCGTCATGTTGCTGTTGAAACTGACCCGCGTGGTGAACGTAGATATTTGGAATATCTGGCATATGACCTTCACCGGCGCCATTGTGCATATCGCCa                                                                                                                               >  1:403453/1‑250 (MQ=255)                                                                                                            attcataaatattccttattacagcaAAAGCGATGGTTCAGGCATTTGATATTAATTTGAATGTGTTGGATATCGGCTGGCCGGGCGCCGCACCGATTACTTGGGCATCGCAAATTGCGTTAATCGCTATTCCTATCGCAATCGCGGTGAATATCGTCATGTTGCTGTTGAAACTGACCCGCGTGGTGAACGTAGATATTTGGAATATCTGGCATATGACCTTCACCGGCGCCATTGTGCATATCGCCAc                                                                                                                              >  1:826402/22‑250 (MQ=255)                                                                                                                ggATTCTATCGGACCGGCAGCAAAAGCGATGGTGCAGGCATTTGATATTAATTTGAATGTGGTGGATATCGGCTGGCCGGGCGCCGCACCGATTACTTGGGCATCGCAAATTGCGTTAATCGCTATTCCTATCGCAATCGCGGTGAATATCGTCATGTTGCTGTTGAAACTGACCCGCGTGGTGAACGTAGATATTTGGAATATCTGGCATATGACCTTCACCGGCGCCATTGTGCat                                                                                                                                      <  1:278717/238‑1 (MQ=255)                                                                                                                  aTTCTATCGGACCGGCAGCAAAAGCGATGGTGCAGGCATTTGATATTAATTTGAATGTGGTGGATATCGGCTGGCCGGGCGCCGCACCGATTACTTGGGCATCGCAAATTGCGTTAATCGCTATTCCTATCGCAATCGCGGTGAATATCGTCATGTTGCTGTTGAAACTGACCCGCGTGGTGAACGTAGATATTTGGAATATCTGGCATATGACCTTCACCGGCGCCATTGTGCATATCGCCACCGGTTc                                                                                                                        <  1:695278/250‑1 (MQ=255)                                                                                                                     cTATCGGACCGGCAGCAAAAGCGATGGTGCAGGCATTTGATATTAATTTGAATGTGGTGGATATCGGCTGGCCGGGCGCCGCACCGATTACTTGGGCATCGCAAATTGCGTTAATCGCTATTCCTATCGCAATCGCGGTGAATATCGTCATGTTGCTGTTGAAACTGACCCGCGTGGTGAACGTAGATATTTGGAATATCTGGCATATGACCTTCACCGGCGCCATTGTGCat                                                                                                                                      >  1:44084/1‑233 (MQ=255)                                                                                                                         cGGACCGGCAGCAAAAGCGATGGTGCAGGCATTTGATATTAATTTGAATGTGGTGGATATCGGCTGGCCGGGCGCCGCACCGATTACTTGGGCATCGCAAATTGCGTTAATCGCTATTCCTATCGCAATCGCGGTGAATATCGTCATGTTGCTGTTGAAACTGACCCGCGTGGTGAACGTAGATATTTGGAATATCTGGCATATGACCTTCACCGGCGCCATTGTGCATATCGCCACCGGTTCTTATTGGc                                                                                                                <  1:515592/251‑1 (MQ=255)                                                                                                                         cGGACCGGCAGCAAAAGCGATGGTGCAGGCATTTGATATTAATTTGAATGTGGTGGATATCGGCTGGCCGGGCGCCGCACCGATTACTTGGGCATCGCAAATTGCGTTAATCGCTATTCCTATCGCAATCGCGGTGAATATCGTCATGTTGCTGTTGAAACTGACCCGCGTGGTGAACGTAGATATTTGGAATATCTGGCATATGACCTTCACCGGCGCCATTGTGCATATCGCCACCGGTTCTTATTGGc                                                                                                                <  1:9109/251‑1 (MQ=255)                                                                                                                         cGGACCGGCAGCAAAAGCGATGGTGCAGGCATTTGATATTAATTTGAATGTGGTGGATATCGGCTGGCCGGGCGCCGCACCGATTACTTGGGCATCGCAAATTGCGTTAATCGCTATTCCTATCGCAATCGCGGTGAATATCGTCATGTTGCTGTTGAAACTGACCCGCGTGGTGAACGTAGATATTTGGAATATCTGGCATATGACCTTCACCGGCGCCATTGTGCATATCGCCACCGGTTCTTATTGGc                                                                                                                <  1:511742/251‑1 (MQ=255)                                                                                                                         cGGACCGGCAGCAAAAGCGATGGTGCAGGCATTTAATATTAATTTGAATGTGGTGGATATCGGCTGGCCGGGCGCCGCACCGATTACTTGGGCATCGCAAATTGCGTTAATCGCTATTCCTATCGCAATCGCGGTGAATATCGTCATGTTGCTGTTGAAACTGACCCGCGTGGTGAACGTAGATATTTGGAATATCTGGCATATGACCTTCACCGGCGCCATTGTGCATATCGCCACCGGTTCTTATTGGc                                                                                                                <  1:587204/251‑1 (MQ=255)                                                                                                                             ccGGCAGCAAAAGCGATGGTGCAGGCATTTGATATTAATTTGAATGTGGTGGATATCGGCTGGCCGGGCGCCGCACCGATTACTTGGGCATCGCAAATTGCGTTAATCGCTATTCCTATCGCAATCGCGGTGAATATCGTCATGTTGCTGTTGAAACTGACCCGCGTGGTGAACGTAGATATTTGGAATATCTGGCATATGACCTTCACCGGCGCCATTGTGCATATCGCCACCGGTTCTTATTGGCTggg                                                                                                            <  1:883110/251‑1 (MQ=255)                                                                                                                                               gtgcAGGCATTTGATATTAATTTGAATGTGGTGGATATCGGCTGGCCGGGCGCCGCACCGATTACTTGGGCATCGCAAATTGCGTTAATCGCTATTCCTATCGCAATCGCGGTGAATATCGTCATGTTGc                                                                                                                                                                                                                   <  1:665724/128‑1 (MQ=255)                                                                                                                                               gtgcAGGCATTTGATATTAATTTGAATGTGGTGGATATCGGCTGGCCGGGCGCCGCACCGATTACTTGGGCATCGCAAATTGCGTTAATCGCTATTCCTATCGCAATCGCGGTGAATATCGTCATGTTGCTGTTGAAACTGACCCGCGTGGTGAACGTAGATATTTGGAGTATCTGGCATATGa                                                                                                                                                             >  1:456184/3‑184 (MQ=255)                                                                                                                                               gtgcAGGCATTTGATATTAATTTGAATGTGGTGGATATCGGCTGGCCGGGCGCCGCACCGATTACTTGGGCATCGCAAATTGCGTTAATCGCTATTCCTATCGCAATCGCGGTGAATATCGTCATGTTGCTGTTGAAACTGACCCGCGTGGTGAACGTAGATATTTGGAATATCTGGCATATGACCTTc                                                                                                                                                        >  1:179485/3‑189 (MQ=255)                                                                                                                                               gtgcAGGCATTTGATATTAATTTGAATGTGGTGGATATCGGCTGGCCGGGCGCCGCACCGATTACTTGGGCATCGCAAATTGCGTTAATCGCTATTCCTATCGCAATCGCGGTGAATACCGTCATGTTGCTGTTGAAACTGACCCGCGTGGTGAACGTAGATATTTGGAATATCTGGCATATGACCTTCACCGGCGCCATTGTGCATATCg                                                                                                                                  >  1:667357/3‑211 (MQ=255)                                                                                                                                                tgcAGGCATTTGATATTAATTTGAATGTGGTGGATATCGGCTGGCCGGGCGCCGCACCGATTACTTGGGCATCGCAAATTGCGTTAATCGCTATTCCTATCGCAATCGCGGTGAATATCGTCATGTTGCTGTTGAAACTGACCCGCGTGGTGAACGTAGATATTTGGAATATCTGGCATATGACCTTCACCGGCGCCATTGTGCATATCGCCACCGGTTCTTATTGGCTGGGTATTCTCGGGGTTGTGAtt                                                                                         <  1:498300/250‑1 (MQ=255)                                                                                                                                                tgcAGGCATTTGATATTAATTTGAATGTGGTGGATATCGGCTGGCCGGGCGCCGCACCGATTACTTGGGCATCGCAAATTGCGTTAATCGCTATTCCTATCGCAATCGCGGTGAATATCGTCATGTTGCTGTTGAAACTGACCCGCGTGGTGAACGTAGATATTTGGAATATCTGGCATATGACCTTCACCGGCGCCATTGTGCATATCGCCACCGGTTCTTATTGGCTGGGTATTCTCGGGGTTGTGAtt                                                                                         <  1:715328/250‑1 (MQ=255)                                                                                                                                                     gCATTTGATATTAATTTGAATGTGGTGGATATCGGCTGGCCGGGCGCCGCACCGATTACTTGGGCATCGCAAATTGCGTTAATCGCTATTCCTATCGCAATCGCGGTGAATATCGTCATGTTGCTGTTGAAACTGACCCGCGTGGTGAACGTAGATATTTGGAATATCTGGCATATGACCTTCACCGGCGCCATTGTGCATATCGCCACCGGTTCTTATTGGCTGGGTATTCTCGGGGTTGTGATTCACg                                                                                     >  1:333896/1‑250 (MQ=255)                                                                                                                                                      cATTTGATATTAATTTGAATGTGGTGGATATCGGCTGGCCGGGCGCCGCACCGATTACTTGGGCATCGCAAATTGCGTTAATCGCTATTCCTATCGCAATCGCGGTGAATATCGTCATGTTGCTGTTGAAACTGACCCGCGTGGTGAACGTAGATATTTGGAATATCTGGCATATGAc                                                                                                                                                            <  1:8651/178‑1 (MQ=255)                                                                                                                                                        tttGATATTAATTTGAATGTGGTGGATATCGGCTGGCCGGGCGCCGCACCGATTACTTGGGCATCGCAAATTGCGTTAATCGCTATTCCTATCGCAATCGCGGTGAATATCGTc                                                                                                                                                                                                                          <  1:551696/114‑1 (MQ=255)                                                                                                                                                          tGATATTAATTTGAATGTGGTGGATATCGGCTGGCCGGGCGCCGCACCGATTACTTGGGCATCGCAAATTGCGTTAATCGCTATTCCTATCGCAATCGCGGTGAATATCGTCATGTTGCTGTTGAAACTGACCCGCGTGGTGAACGTAGATATTTGGAATATCTGGCATATGACCTTCACCGGCGCCATTGTGCATATCGCCACCGGTTCTTATTGGCTGGGTATTCTCGGGGTTGTGATTCACGCTGCgt                                                                               <  1:703507/251‑1 (MQ=255)                                                                                                                                                              atTAATTTGAATGTGGTGGATATCGGCTGGCCGGGCGCCGCACCGATTACTTGGGCATCGCAAATTGCGTTAATCGCTATTCCTATCGCAATCGCGGTGAATATCGTCATGTTGCTGTTGAAACTGACCCGCGTGGTGAACGTAGATATTTGGAATATCTGGCATATGACCTTCACCGGCGCCATTGTGCATATCGCCACCGGTTCTTATTGGCTGGGTATTCTCGGGGTTGTGATTCACGCTGCgtttgt                                                                           <  1:498690/251‑1 (MQ=255)                                                                                                                                                               ttAATTTGAATGTGGTGGATATCGGCTGGCCGGGCGCCGCACCGATTACTTGGGCATCGCAAATTGCGTTAATCGCTATTCCTATCGCAATCGCGGTGAATATCGTCATGTTGCTGTTGAAACTGACCCGCGTGGTGAACGTAGATATTTGGAATATCTGGCATATGACCTTCACCGGCGCCATTGTGCATATCGCCACCGGTTCTTATTGGCTGGGTATTCTCGGGGTTGTGATTCACGCTGCgtttgtt                                                                          <  1:616184/251‑1 (MQ=255)                                                                                                                                                                    ttGAATGTGGTGGATATCGGCTGGCCGGGCGCCGCACCGATTACTTGGGCATCGCAAATTGCGTTAATCGCTATTCCTATCGCAATCGCGGTGAATATCGTCATGTTGCTGTTGAAACTGACCCGCGTGGTGAACGTAGATATTTGGAATATCTGGCATATGACCTTCACCGGCGCCATTGTGCATATCGCCACCGGTTCTTATTGGCTGGGTATTCTCGGGGTTGTGATTCACGCTGCGTTTGTTTACaa                                                                     <  1:508907/251‑1 (MQ=255)                                                                                                                                                                          gtggtgGATATCGGCTGGCCGGGCGCCGCACCGATTACTTGGGCATCGCAAATTGCGTTAATCGCTATTCCTATCGCAATCGCGGTGAATATCGTCATGTTGCTGTTGAAACTGACCCGCgtggtg                                                                                                                                                                                            <  1:35762/126‑1 (MQ=255)                                                                                                                                                                          gtggtgGATATCGGCTGGCCGGGCGCCGCACCGATTACTTGGGCATCGCAAATTGCGTTAATCGCTATTCCTATCGCAATCGCGGTGAATATCGTCATGTTGCTGTTGAAACTGACCCGCGTGGTGAACGTAGATATTTGGaa                                                                                                                                                                           >  1:478393/1‑143 (MQ=255)                                                                                                                                                                          gtggtgGATATCGGCTGGCCGGGCGCCGCACCGATTACTTGGGCATCGCAAATTGCGTTAATCGCTATTCCTATCGCAATCGCGGTGAATATCGTCATGTTGCTGTTGAAACTGACCCGCGTGGTGAACGTAGATATTTGGAATATCTGGCATATGACCTTCACCGGCGCCATTGTGCATATCGCCACCGGTTCTTATTGGCTGGGTATTCTCGGGGTTGTGATTCACGCTGCGTTTGTTTACAAACTggg                                                               >  1:206830/1‑251 (MQ=255)                                                                                                                                                                          gtggtgGATATCGGCTGGCCGGGCGCCGCACCGATTACTTGGGCATCGCAAATTGCGTTAATCGCTATTCCTATCGCAATCGCGGTGAATATCGTCATGTTGCTGTTGAAACTGACCCGCGTGGTGAACGTAGATATTTGGAATATCTGGCATATGACCTTCACCGGCGCCATTGTGCATATCGCCACCGGTTCTTATTGGCTGGGTATTCTCGGGGTTGTGATTCACGCTGCGTTTGTTTACAAACTggg                                                               >  1:378352/1‑251 (MQ=255)                                                                                                                                                                             gtggATATCGGCTGGCCGGGCGCCGCACCGATTACTTGGGCATCGCGAATTGCGTTAATCGCTATTCCTATCGCAATCGCGGTGAATATCGTCATGTTGCTGTTGAAACTGACCCGCGTGGTGAACGTAGATATTTGGAATATCTGGCATATGACCTTCACCGGCGCCATTGTGCATATCGCCACCGGTTCTTATTGGCTGGGTATTCTCGGGGTTGTGATTCACGCTGCGTTTGTTTACAAACTGGgag                                                             <  1:613020/250‑1 (MQ=255)                                                                                                                                                                              tggATATCGGCTGGCCGGGCGCCGCACCGATTACTTGGGCATCGCAAATTGCGTTAATCGCTATTCCTATCGCAATCGCGGTGAATATCGTCATGTTGCTGTTGAAACTGACCCGCGTGGTGAACGTAGATATTTGGAATATCTGGCATATGACCTTCACCGGCGCCATTGTGCATATCGCCACCGGTTCTTATTGGCTGGGTATTCTCGGGGTTGTGATTCACGCTGCGTTTGTTTACAAACTGGGAGAt                                                           <  1:85130/251‑1 (MQ=255)                                                                                                                                                                               ggATATCGGCTGGCCGGGCGCCGCACCGATTACTTGGGCATCGCAAATTGCGTTAATCGCTATTCCTATCGCAATCGCGGTGAATATCGTCATGTTGCTGTTGAAACTGACCCGCGTGGTGAACGTAGATATTTGGAATATCTGGCATATGACCTTCACCGGCGCCATTGTGCATATCGCCACCGGTTCTTATTGGCTGGGTATTCTCGGGGt                                                                                                <  1:655778/213‑1 (MQ=255)                                                                                                                                                                                 atatCGGCTGGCCGGGCGCCGCACCGATTACTTGGGCATCGCAAATTGCGTTAATCGCTATTCCTATCGCAATCGCGGTGAATATCGTCATGTTGCTGTTGAAACTGACCCGCGTGGTGAACGTAGATATTTGGAATATCTGGCATATGACCTTCACCGGCGCCATTGTGCATATCGCCACCGGTTCTTATTGGCTGGGTATTCTCGGGGTTGTGATTCACGCTGCGTTTGTTTACAAACTGGGAGATTgg                                                        <  1:571864/251‑1 (MQ=255)                                                                                                                                                                                                   ccgcACCGATTACTTGGGCATCGCAAATTGCGTTAATCGCTATTCCTATCGCAATCGCGGTGAATATCGTCATGTTGCTGTTGAAACTGACCCGCGTGGTGAACGTAGATATTTGGAATATCTGGCATATGACCTTCACCGGCGCCATTGTGCat                                                                                                                                      >  1:439866/1‑155 (MQ=255)                                                                                                                                                                                                   ccgcACCGATTACTTGGGCATCGCAAATTGCGTTAATCGCTATTCCTATCGCAATCGCGGTGAATATCGTCATGTTGCTGTTGAAACTGACCCGCGTGGTGAACGTAGATATTTGGAATATCTGGCATATGACCTTCACCGGCGCCATTGTGCATATCGCCACCGGTTCTTATTGGCTGGGTATTCTCGGGGTTGTGATTCACGCTGCGTTTGTTTACAAACTGGGAGATTGGTTTAGTCGCGATACGaaa                                      <  1:791177/251‑1 (MQ=255)                                                                                                                                                                                                        ccGATTACTTGGGCATCGCAAATTGCGTTAATCGCTATTCCTATCGCAATCGCGGTGAATATCGTCATGTTGCTGTTGAAACTGACCCGCgtggtg                                                                                                                                                                                            <  1:54614/96‑1 (MQ=255)                                                                                                                                                                                                         cGATTACTTGGGCATCGCAAATTGCGTTAATCGCTATTCCTATCGCAATCGCGGTGAATATCGTCATGTTGCTGTTGAAACTGACCCGCGTGGTGAACGTAGATATTTGGAATATCTGGCATATGACCTTCACCGGCGCCATTGTGCATATCGCCACCGGTTCTTATTGGCTGGGTATTCTCGGGGTTGTGATTCACGCTGCGTTTGTTTACAAACTGGGAGATTGGttt                                                     >  1:22596/1‑230 (MQ=255)                                                                                                                                                                                                          gATTACTTGGGCATCGCAAATTGCGTTAATCGCTATTCCTATCGCAATCGCGGTGAATATCGTCATGTTGCTGTTGAAACTGACCCGCGTGGTGAACGTAGATATTTGGAATATCTGGCATATGACCTTCACCGGCGCCATTGTGCATATCGCCACCGGTTCTTATTGGCTGGGTATTCTCGGGGTTGTGATTCACGCTGCGTTTGTTTACAAACTggg                                                               <  1:534909/219‑1 (MQ=255)                                                                                                                                                                                                          gATTACTTGGGCATCGCAAATTGCGTTAATCGCTATTCCTATCGCAATCGCGGTGAATATCGTCATGTTGCTGTTGAAACTGACCCGCGTGGTGAACGTAGATATTTGGAATATCTGGCATATGACCTTCACCGGCGCCATTGTGCATATCGCCACCGGTTCTTATTGGCTGGGTATTCTCGGGGTTGTGATTCACGCTGCGTTTGTTTACAAACTGGGAGATTGGTTTAGTCGCGATACGAAAAAttt                                 >  1:874804/1‑249 (MQ=255)                                                                                                                                                                                                          gATTACTTGGGCATCGCAAATTGCGTTAATCGCTATTCCTATCGCAATCGCGGTGAATATCGTCATGTTGCTGTTGAAACTGACCCGCGTGGTGAACGTAGATATTTGGAATATCTGGCATATGACCTTCACCGGCGCCATTGTGCATATCGCCACCGGTTCTTATTGGCTGGGTATTCTCGGGGTTGTGATTCACGCTGCGTTTGTTTACAAACTGGGAGATTGGTTTAGTCGCGATACGAAAAAttt                                 >  1:741063/1‑249 (MQ=255)                                                                                                                                                                                                             tACTTGGGCATCGCAAATTGCGTTAATCGCTATTCCTATCGCAATCGCGGTGAATATCGTCATGTTGCTGTTGAAACTGACCCGCGTGGTGAACGTAGATATTTGGAATATCTGGCATATGACCTTCACCGGCGCCATTGTGCat                                                                                                                                      <  1:470026/145‑1 (MQ=255)                                                                                                                                                                                                                          cAAATTGCGTTAATCGCTATTCCTATCGCAATCGCGGTGAATATCGTCATGTTGCTGTTGAAACTGACCCGCGTGGTGAACGTAGATATTTGGAATATCTGGCATATGACCTTCACCGGCGCCATTGTGCATATCGCCACCGGTTCTTATTGGCTGGGTATTCTCGGGGTTGTGATTCACGCTGCGTTTGTTTACAAACTGGGAGATTGGTTTAGTCGCGATACGAAAAATTTCTTTGAACTGGAc                    >  1:196976/1‑246 (MQ=255)                                                                                                                                                                                                                          cAAATTGCGTTAATCGCTATTCCTATCGCAATCGCGGTGAATATCGTCATGTTGCTGTTGAAACTGACCCGCGTGGTGAACGTAGATATTTGGAATATCTGGCATATGACCTTCACCGGCGCCATTGTGCATATCGCCACCGGTTCTTATTGGCTGGGTATTCTCGGGGTTGTGATTCACGCTGCGTTTGTTTACAAACTGGGAGATTGGTTTAGTCGCGATACGAAAAATTTCTTTGAACTGGAc                    >  1:2747/1‑246 (MQ=255)                                                                                                                                                                                                                               tGCGTTAATCGCTATTCCTATCGCAATCGCGGTGAATATCGTCATGTTGCTGTTGAAACTGACCCGCGTGGTGAACGTAGATATTTGGAATATCTGGCATATGACCTTCACCGGCGCCATTGTGCATATCGCCACCGGTTCTTATTGGCTGGGTATTCTCGGGGTTGTGATTCACGCTGCGTTTGTTTACAAACTGGGAGATTGGTTTAGTCGCGATACGAAAAATTTCTTTGAACTGGACGGCATTGCCa          <  1:47673/251‑1 (MQ=255)                                                                                                                                                                                                                                gCGTTAATCGCTATTCCTATCGCAATCGCGGTGAATATCGTCATGTTGCTGTTGAAACTGACCCGCGTGGTGAACGTAGATATTTGGAATATCTGGCATATGACCTTCACCGGCGCCATTGTGCATATCGCCACCGGTTCTTATTGGCTGGGTATTCTCGGGGTTGTGATTCACGCTGCGTTTGTTTACAAACTg                                                                 >  1:362974/1‑195 (MQ=255)                                                                                                                                                                                                                                   ttAATCGCTATTCCTATCGCAATCGCGGTGAATATCGTCATGTTGCTGTTGAAACTGACCCGCGTGGTGAACGTAGATATTTGGAATATCTGGCATATGACCTTCACCGGCGCCATTGTGCATATCGCCACCGGTTCTTATTGGCTGGGTATTCTCGGGGTTGTGATTCACGCTGCGTTTGTTTACAAACTGGGAGATTGGTTTAGTCGCGATACGAAAAATTTCTTTGAACTGGACGGCATTGCCATTc       <  1:649524/250‑1 (MQ=255)                                                                                                                                                                                                                                     aaTCGCTATTCCTATCGCAATCGCGGTGAATATCGTCATGTTGCTGTTGAAACTGACCCGCGTGGTGAACGTAGATATTTGGAATATCTGGCATATGACCTTCACCGGCGCCATTGTGCATATCGCCACCGGTTCTTATTGGCTGGGTATTCTCGGGGTTGTGATTCACGCTGCGTTTGTTTACAAACTGGGAGATTGGTTTAGTCGCGATACGAAAAATTTCTTTGAACTGGACGGCATTGCCATTCCg     >  1:183842/1‑250 (MQ=255)                                                                                                                                                                                                                                       tCGCTATTCCTATCGCTATCGCGGTGAATATCGTCATGTTGCTGTTGAAACTGACCCTCGTGGTGAACGTAGATATTTGGAATATCTGGCATATGACCTTCACCGGCGCCATTGTGCATATCGCCACCGGTTCTTATTGGCTGGGTATTCTCGGGGTTGTGATTCACGCTGCGTTTGTTTACAAACTGGGAGATTGGTTTAGTCGCGATACGAAAAATTTCTTTGAACTGGACGGCATTGCCATTCCGCAc  <  1:302748/251‑1 (MQ=255)                                                                                                                                                                                                                                       tCGCTATTCCTATCGCAATCGCGGTGAATATCGTCATGTTGCTGTTGAAACTGACCCGCGTGGTGAACGTAGATATTTGGAATATCTGGCATATGAc                                                                                                                                                            <  1:762210/97‑1 (MQ=255)                                                                                                                                                                                                                                       tCGCTATTCCTATCGCAATCGCGGTGAATATCGTCATGTTGCTGTTGAAACTGACCCGCGTGGTGAACGTAGATATTTGGAATATCTGGCATATGACCTTCACCGGCGCCATTGTGCATATCGCCACCGGTTCTTATTGGCTGGGTATTCTCGGGGTTGTGAtt                                                                                         <  1:333686/164‑1 (MQ=255)                                                                                                                                                                                                                                       tCGCTATTCCTATCGCAATCGCGGTGAATATCGTCATGTTGCTGTTGAAACTGACCCGCGTGGTGAACGTAGATATTTGGAATATCTGGCATATGACCTTCACCGGCGCCATTGTGCATATCGCCACCGGTTCTTATTGGCTGGGTATTCTCGGGGTTGTGATTCACGCTGCGTTTGTTTACAAACTGGGAGATTGGTTTAGTCGCGATACGAAAAATTTCTTTGAACTGGACGGCATTGCCATTc       <  1:660832/246‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                             GATTATTATTCTTTCGTTGTTGCTCGGCATGAAAGCCGGCGATTCCTTTAAGGCCGGATTACAAATCGGTATTGGCTTTGTAGGGATCGGGCTGGTTATCGGCTTAATGCTGGATTCTATCGGACCGGCAGCAAAAGCGATGGCGCAGGCATTTGATATTAATTTGAATGTGGTGGATATCGGCTGGCCGGGCGCCGCACCGATTACTTGGGCATCGCAAATTGCGTTAATCGCTATTCCTATCACAATCGCGGTGAATATCGTCATGTTGCTGTTGAAACTGACCCGCGTGGTGAACGTAGATATTTGGAATATCTGGCATATGACCTTCACCGGCGCCATTGTGCATATCGCCACCGGTTCTTATTGGCTGGGTATTCTCGGGGTTGTGATTCACGCTGCGTTTGTTTACAAACTGGGAGATTGGTTTAGTCGCGATACGAAAAATTTCTTTGAACTGGACGGCATTGCCATTCCGCAC  >  1/1238888‑1239368

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑