Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,229,702	T→C	intergenic (+90572/+163609)	unknown → / ← unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,229,702	0	T	C	100.0%	205.3 / NA	68	intergenic (+90572/+163609)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (38/30);  total (38/30)

GCAAAACGGGATCATCGTAAAATCGGTAAAGCATTGGATTTATATCACATGCAGGAAGAAGCGCCGGGTATGGTGTTCTGGCACAATGACGGCTGGACGATTTTCCGTGAACTGGAAACTTTCGTGCGCACTAAATTGAAACAATATGACTATCAGGAAGTGAAAGGTCCGTTCATGATGGATCGCGTGTTGTGGGAAAAAACCGGTCACTGGCAAAACTACGGCGATTTGATGTTCACCACCTTTTCTGAAAACCGCGAATATGCCATCAAACCGATGAACTGCCCGGGACACGTTCAGATCTTCAATCAAGGTTTAAAATCTTATCGCGATTTACCGCTACGTATGGCGGAATTCGGTTCCTGCCACCGTAACGAACCGTCCGGTTCATTGCACGGTTTAATGCGCGTGCGCGGTTTTACTCAAGATGACGCTCACATTTTCTGTATGCCGGATCAAGTGGAAAGCGAAGTGACTTCCTGCA  >  1/1229458‑1229941                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                gCAAAACGGGATCATCGTAAAATCGGTAAAGCATTGGATTTATATCACATGCAGGAAGAAGCGCCGGGTATGGTGTTCTGGCACAATGACGGCTGGACGATTTTCCGTGAACTGGAAACTTTCGTGCGCACTAAATTGAAACAATATGACTATCAGGAAGTGAAAGGTCCGTTCATGATGGATCGCGTGTTGTGGGAAAAAACCGGTCACTGGCAAAACTACGGCGATTTGATGTTCACCACCTCTTCTg                                                                                                                                                                                                                                            <  1:382426/250‑1 (MQ=255)   aaaaCGGGATCATCGTAAAATCGGTAAAGCATTGGATTTATATCACATGCAGGAAGAAGCGCCGGGTATGGTGTTCTGGCATAATGACGGCTGGACGATTTTCCGTGAACTGGAAACTTTCGTGCGCACTAAATTGAAACAATATGACTATCAGGAAGTGAAAGGTCCGTTCATGATGGATCGCGTGTTGTGGGAAAAAACCGGTCACTGGCAAAACTACGGCGATTTGATGTTCACCACCTCTTCTGaaa                                                                                                                                                                                                                                         <  1:427684/251‑1 (MQ=255)   aaaaCGGGATCATCGTAAAATCGGTAAAGCATTGGATTTATATCACATGCAGGAAGAAGCGCCGGGTATGGTGTTCTGGCACAATGACGGCTGGACGATTTTCCGTGAACTGGAAACTTTCGTGCGCACTAAATTGAAACAATATGACTATCAGGAAGTGAAAGGTCCGTTCATGATGGATCGCGTGTTGTGGGAAAAAACCGGTCACTGGCAAAACTACGGCGATTTGATGTTCACCACCTCTTCTGaaa                                                                                                                                                                                                                                         <  1:627236/251‑1 (MQ=255)     aaCGGGATCATCGTAAAATCGGTAAAGCATTGGATTTATATCACATGCAGGAAGAAGCGCCGGGTATGGTGTTCTGGCACAATGACGGCTGGACGATTTTCCGTGAACTGGAAACTTTCGTGCGCACTAAATTGAAACAATATGACTATCAGGAAGTGAAAGGTCCGTTCATGATGGATCGCGTGTTGTGGGAAAAAACCGGTCACTGGCAAAACTACGGCGATTTGATGTTCACCACCTCTTCTGAAAAc                                                                                                                                                                                                                                       <  1:728585/251‑1 (MQ=255)               tcGTAAAATCGGTAAAGCATTGGATTTATATCACATGCAGGAAGAAGCGCCGGGTATGGTGTTCTGGCACAATGACGGCTGGACGATTTTCCGTGAACTGGAAACTTTCGTGCGCACTAAATTGAAACAATATGACTATCAGGAAGTGAAAGGTCCGTTCATGATGGATCGCGTGTTGTGGGAAAAAACCGGTCACTGGCAAAACTACGGCGATTTGATGTTCACCACCTCTTCTGAAAACCGCGAATATg                                                                                                                                                                                                                             >  1:27822/1‑251 (MQ=255)                          gTAAAGCATTGGATTTATATCACATGCAGGAAGAAGCGCCGGGTATGGTGTTCTGGCACAATGACGGCTGGACGATTTTCCGTGAACTGGAAACTTTCGTGCGCACTAAATTGAAACAATATGACTATCAGGAAGTGAAAGGTCCGTTCATGATGGATCGCGTGTTGTGGGAAAAAACCGGTCACTGGCAAAACTACGGCGATTTGATGTTCACCACCTCTTCTGAAAACCGCGAATATGCCATCAAAcc                                                                                                                                                                                                                   <  1:154061/250‑1 (MQ=255)                               gCATTGGATTTATATCACATGCAGGAAGAAGCGCCGGGTATGGTGTTCTGGCACAATGACGGCTGGACGATTTTCCGTGAACTGGAAACTTTCGTGCGCACTAAATTGAAACAATATGACTATCAGGAAGTGAAAGGTCCGTTCATGATGGATCGCGTGTTGTGGGAAAAAACCGGTCACTGGCAAAACTACGGCGATTTGATGTTCACCACCTCTTCTGa                                                                                                                                                                                                                                           >  1:592108/1‑221 (MQ=255)                                cATTGGATTTATATCACATGCAGGAAGAAGCGCCGGGTATGGTGTTCTGGCACAATGACGGCTGGACGATTTTCCGTGAACTGGAAACTTTCGTGCGCACTAAATTGAAACAATATGACTATCAGGAAGTGAAAGGTCCGTTCATGATGGATCGCGTGTTGTGGGAAAAAACCGGTCACTGGCAAAACTACGGCGATTTGATGTTCACCACCTCTTCTGAAAACCGCGAATATGCCATCAAACCGATGAAc                                                                                                                                                                                                            <  1:796130/251‑1 (MQ=255)                                    ggATTTATATCACATGCAGGAAGAAGCGCCGGGTATGGTGTTCTGGCACAATGACGGCTGGACGATTTTCCGTGAACTGGAAACTTTCGTGCGCACTAAATTGAAACAATATGACTATCAGGAAGTGAAAGGTCCGTTCATGATGGATCGCGTGTTGTGGGAAAAAACCGGTCACTGGCAAAACTACGGCGATTTGATGTTCACCACCTCTTCTGAAAACCGCGAATATGCCATCAAACCGATGAACTGc                                                                                                                                                                                                         >  1:856390/1‑250 (MQ=255)                                     gATTTATATCACATGCAGGAAGAAGCGCCGGGTATGGTGTTCTGGCACAATGACGGCTGGACGATTTTCCGTGAACTGGAAACTTTCGTGCGCACTAAATTGAAACAATATGACTATCAGGAAGTGAAAGGTCCGTTCATGATGGATCGCGTGTTGTGGGAAAAAACCGGTCACTGGCAAAACTACGGCGATTTGATGTTCACCACCTCTTCTGAAAACCGCGAATATGCCATCAAACCGATGAACTGcc                                                                                                                                                                                                        >  1:763750/1‑250 (MQ=255)                                          atatCACATGCAGGAAGAAGCGCCGGGTATGGTGTTCTGGCACAATGACGGCTGGACGATTTTCCGTGAACTGGAAACTTTCGTGCGCACTAAATTGAAACAATATGACTATCAGGAAGTGAAAGGTCCGTTCATGATGGATCGCGTGTTGTGGGAAAAAACCGGTCACTGGCAAAACTACGGCGATTTGATGTTCACCACCTCTTCTGAAAACCGCGAATATGCCATCAAACCGATGAACTGCCCGGGac                                                                                                                                                                                                  <  1:4337/251‑1 (MQ=255)                                          atatCACATGCAGGAAGAAGCGCCGGGTATGGTGTTCTGGCACAATGACGGCTGGACGATTTTCCGTGAACTGGAAACTTTCGTGCGCACTAAATTGAAACAATATGACTATCAGGAAGTGAAAGGTCCGTTCATGATGGATCGCGTGTTGTGGGAAAAAACCGGTCACTGGCAAAACTACGGCGATTTGATGTTCACCACCTCTTCTGAAAACCGCGAATATGCCATCAAACCGATGAACTGCCCGGGac                                                                                                                                                                                                  <  1:197989/251‑1 (MQ=255)                                                   gCAGGAAGAAGCGCCGGGTATGGTGTTCTGGCACAATGACGGCTGGACGATTTTCCGTGAACTGGAAACTTTCGTGCGCACTAAATTGAAACAATATGACTATCAGGAAGTGAAAGGTCCGTTCATGATGGATCGCGTGTTGTGGGAAAAAACCGGTCACTGGCAAAACTACGGCGATTTGATGTTCACCACCTCTTCTGAAAACCGCGAATATGCCATCAAACCGATGAACTGCCCGGGACACGTTCAGa                                                                                                                                                                                         >  1:179429/1‑251 (MQ=255)                                                              cgcCGGGTATGGTGTTCTGGCACAATGACGGCTGGACGATTTTCCGTGAACTGGAAACTTTCGTGCGCACTAAATTGAAACAATATGACTATCAGGAAGTGAAAGGTCCGTTCATGATGGATCGCGTGTTGTGGGAAAAAACCGGTCACTGGCAAAACTACGGCGATTTGATGTTCACCACCTCTTCTGAAAACCGCGAATATGCCATCAAACCGATGAACTGCCCGGGACACGTTCAGATCTtcaatca                                                                                                                                                                               <  1:737154/250‑1 (MQ=255)                                                              cgcCGGGTATGGTGTTCTGGCACAATGACGGCTGGACGATTTTCCGTGAACTGGAAACTTTCGTGCGCACTAAATTGAAACAATATGACTATCAGGAAGTGAAAGGTCCGTTCATGATGGATCGCGTGTTGTGGGAAAAAACCGGTCACTGGCAAAACTACGGCGATTTGATGTTCACCACCTCTTCTGAAAACCGCGAATATGCCATCAAACCGATGAACTGCCCGGGACACGTTCAGATCTtcaatc                                                                                                                                                                                <  1:777619/249‑1 (MQ=255)                                                                  gggTATGGTGTTCTGGCACAATGACGGCTGGACGATTTTCCGTGAACTGGAAACTTTCGTGCGCACTAAATTGAAACAATATGACTATCAGGAAGTGAAAGGTCCGTTCATGATGGATCGCGTGTTGTGGGAAAAAACCGGTCACTGGCAAAACTACGGCGATTTGATGTTCACCACCTCTTCTGAAAACCGCGAATATGCCATCAAACCGATGAACTGCCCGGGACACGTTCAGAt                                                                                                                                                                                        >  1:706547/1‑237 (MQ=255)                                                                   ggTATGGTGTTCTGGCACAATGACGGCTGGACGATTTTCCGTGAACTGGAAACTTTCGTGCGCACTAAATTGAAACAATATGACTATCAGGAAGTGAAAGGTCCGTTCATGATGGATCGCGTGTTGTGGGAAAAAACCGGTCACTGGCAAAACTACGGCGATTTGATGTTCACCACCTCTTCTGAAAACCGCGAATATGCCATCAAACCGATGAACTGCCCGGGACACGTTCAGATCTTCAATCAAGGttt                                                                                                                                                                         >  1:603658/1‑251 (MQ=255)                                                                      aTGGTGTTCTGGCACAATGACGGCTGGACGATTTTCCGTGAACTGGAAACTTTCGTGCGCACTAAATTGAAACAATATGACTATCAGGAAGTGAAAGGTCCGTTCATGATGGATCGCGTGTTGTGGGAAAAAACCGGTCACTGGCAAAACTACGGCGATTTGATGTTCACCACCTCTTCTGAAAACCGCGAATATGCCATCAAACCGATGAACTGCCCGGGACACGTTCAGATCTtcaatc                                                                                                                                                                                >  1:272670/1‑241 (MQ=255)                                                                         gtgtTCTGGCACAATGACGGCTGGACGATTTTCCGTGAACTGGAAACTTTCGTGCGCACTAAATTGAAACAATATGACTATCAGGAAGTGAAAGGTCCGTTCATGATGGATCGCGTGTTGTGGGAAAAAACCGGTCACTGGCAAAACTACGGCGATTTGATGTTCACCACCTCTTCTGAAAACCGCGAATATGCCATCAAACCGATGAACTGCCCGGGACACGTTCAGATCTTCAATCAAGGTTTAAAAt                                                                                                                                                                    >  1:869150/1‑250 (MQ=255)                                                                                ggCACAATGACGGCTGGACGATTTTCCGTGAACTGGAAACTTTCGTGCGCACTAAATTGAAACAATATGACTATCAGGAAGTGAAAGGTCCGTTCATGATGGATCGCGTGTTGTGGGAAAAAACCGGTCACTGGCAAAACTACGGCGATTTGATGTTCACCACCTCTTCTGaaa                                                                                                                                                                                                                                         >  1:335665/1‑174 (MQ=255)                                                                                ggCACAATGACGGCTGGACGATTTTCCGTGAACTGGAAACTTTCGTGCGCACTAAATTGAAACAATATGACTATCAGGAAGTGAAAGGTCCGTTCATGATGGATCGCGTGTTGTGGGAAAAAACCGGTCACTGGCAAAACTACGGCGATTTGATGTTCACCACCTCTTCTGAAAACCGCGAATATGCCATCAAACCGATGAACTGCCCGGGACACGTTCAGATCTTCAATCAAGGTTTAAAATCTTATcg                                                                                                                                                             >  1:665824/1‑250 (MQ=255)                                                                                    caATGACGGCTGGACGATTTTCCGTGAACTGGAAACTTTCGTGCGCACTAAATTGAAACAATATGACTATCAGGAAGTGAAAGGTCCGTTCATGATGGATCGCGTGTTGTGGGAAAAAACCGGTCACTGGCAAAACTACGGCGATTTGATGTTCACCACCTCTTCTGAAGACCGCGAATATGCCATCAAACCGATGAACTGCCCGGGACACGTTCAGATCTTCAATCAAGGTTTAAAATCTTATCGCGAtt                                                                                                                                                        >  1:426357/1‑251 (MQ=255)                                                                                                 aCGATTTTCCGTGAACTGGAAACTTTCGTGCGCACTAAATTGAAACAATATGACTATCAGGAAGTGAAAGGTCCGTTCATGATGGATCGCGTGTTGTGGGAAAAAACCGGTCACTGGCAAAACTACGGCGATTTGATGTTCACCACCTCTTCTGAAAACCGCGAATATGCCATCAAACCGATGAACTGCCCGGGACACGTTc                                                                                                                                                                                            >  1:575658/1‑202 (MQ=255)                                                                                                    aTTTTCCGTGAACTGGAAACTTTCGTGCGCACTAAATTGAAACAATATGACTATCAGGAAGTGAAAGGTCCGTTCATGATGGATCGCGTGTTGTGGGAAAAAACCGGTCACTGGCAAAACTACGGCGATTTGATGTTCACCACCTCTTCTGAAAACCGCGAATATGCCATCAAACCGATGAACTGCCCGGGACACGTTCAGATCTTCAATCAAGGTTTAAAATCTTATCGCGATTTACCGCTACGTATgg                                                                                                                                         >  1:872142/1‑250 (MQ=255)                                                                                                      tttCCGTGAACTGGAAACTTTCGTGCGCACTAAATTGAAACAATATGACTATCAGGAAGTGAAAGGTCCGTTCATGATGGATCGCGTGTTGTGGGAAAAAACCGGTCACTGGCAAAACTACGGCGATTTGATGTTCACCACCTCTTCTGAAAACCGCGAATATGCCATCAAACCGATGAACTGCCCGGGACACGTTCAGATCTTCAATCAAGGTTTAAAATCTTATCGCGATTTACCGCTACGTATGGCgg                                                                                                                                      <  1:156857/251‑1 (MQ=255)                                                                                                      tttCCGTGAACTGGAAACTTTCGTGCGCACTAAATTGAAACAATATGACTATCAGGAAGTGAAAGGTCCGTTCATGATGGATCGCGTGTTGTGGGAAAAAACCGGTCACTGGCAAAACTACGGCGATTTGATGTTCACCACCTCTTCTGAAAACCGCGAATATGCCATCAAACCGATGAACTGCCCGGGACACGTTCAGATCTTCAATCAAGGTTTAAAATCTTATCGCGATTTACCGCTACGTATGGCgg                                                                                                                                      <  1:341782/251‑1 (MQ=255)                                                                                                                cTGGAAACTTTCGTGCGCACTAAATTGAAACAATATGACTATCAGGAAGTGAAAGGTCCGTTCATGATGGATCGCGTGTTGTGGGAAAAAACCGGTCACTGGCAAAACTACGGCGATTTGATGTTCACCACCTCTTCTGAAAACCGCGAATATGCCATCAAACCGATGAACTGCCCGGGACACGTTCAGATCTTCAATCAAGGTTTAAAATCTTATCGCGATTTACCGCTACGTATGGCGGAATTCGGTTc                                                                                                                            <  1:642473/251‑1 (MQ=255)                                                                                                                    aaaCTTTCGTGCGCACTAAATTGAAACAATATGACTATCAGGAAGTGAAAGGTCCGTTCATGATGGATCGCGTGTTGTGGGAAAAAACCGGTCACTGGCAAAACTACGGCGATTTGATGTTCACCACCTCTTCTGAAAACCGCGAATATGCCATCAAACCGATGAACTGCCCGGGACACGTTCAGATCTTCAATCAAGGTTTAAAATCTTATCGCGATTTACCGCTACGTATGGCGGAATTCGGTTCCTg                                                                                                                         >  1:464870/1‑250 (MQ=255)                                                                                                                        tttCGTGCGCACTAAATTGAAACAATATGACTATCAGGAAGTGAAAGGTCCGTTCATGATGGATCGCGTGTTGTGGGAAAAAACCGGTCACTGGCAAAACTACGGCGATTTGATGTTCACCACCTCTTCTGAAAACCGCGAATATGCCATCAAACCGATGAACTGCCCGGGACACGTTCAGATCTTCAATCAAGGTTTAAAATCTTATCGCGATTTACCGCTACGTATGGCGGAATTCGGTTCCTGCCAcc                                                                                                                    <  1:551170/251‑1 (MQ=255)                                                                                                                          tCGTGCGCACTAAATTGAAACAATATGACTATCAGGAAGTGAAAGGTCCGTTCATGATGGATCGCGTGTTGTGGGAAAAAACCGGTCACTGGCAAAACTACGGCGATTTGATGTTCACCACCTCTTCTGAAAACCGCGAATATGCCATCAAACCGATGAACTGCCCGGGACACGTTCAGATCTTCAATCAAGGTTTAAAATCTTATCGCGATTTACCGCTACGTATGGCGGAATTCGGTTCCTGCCACCGt                                                                                                                  <  1:158665/251‑1 (MQ=255)                                                                                                                                 cACTAAATTGAAACAATATGACTATCAGGAAGTGAAAGGTCCGTTCATGATGGATCGCGTGTTGTGGGAAAAAACCGGTCACTGGCAAAACTACGGCGATTTGATGTTCACCACCTCTTCTGAAAACCGCGAATATGCCATCAAACCGATGAACTGccc                                                                                                                                                                                                       >  1:20331/1‑159 (MQ=255)                                                                                                                                 cACTAAATTGAAACAATATGACTATCAGGAAGTGAAAGGTCCGTTCATGATGGATCGCGTGTTGTGGGAAAAAACCGGTCACTGGCAAAACTACGGCGATTTGATGTTCACCACCTCTTCTGAAAACCGCGAATATGCCATCAAACCGATGAACTGCCCGGGACACGTTCAGATCTTCAATCAAGGTTTAAAATCTTATCGCGATTTACCGCTACGTATGGCGGAATTCGGTTCCTGCCACCGTAACGAAc                                                                                                           <  1:14898/251‑1 (MQ=255)                                                                                                                                   cTAAATTGAAACAATATGACTATCAGGAAGTGAAAGGTCCGTTCATGATGGATCGCGTGTTGTGGGAAAAAACCGGTCACTGGCAAAACTACGGCGATTTGATGTTCACCACCTCTTCTGAAAACCGCGAATATGCCATCAAACCGATGAACTGCCCGGGACACGTTCAGATCTTCAATCAAGGTTTAAAATCTTATCGCGATTTACCGCTACGTATGGCGGAATTCGGTTCCTGCCACCGTAACGAACCg                                                                                                         >  1:389402/1‑251 (MQ=255)                                                                                                                                     aaaTTGAAACAATATGACTATCAGGAAGTGAAAGGTCCGTTCATGATGGATCGCGTGTTGTGGGAAAAAACCGGTCACTGGCAAAACTACGGCGATTTGATGTTCACCACCTCTTCTGAAAACCGCGAATATGCCATCAAACCGATGAACTGCCCGGGACACGTTCAGATCTTCAATCAAGGTTTAAAATCTTATCGCGATTTACCGCTACGTATGGCGGAATTCGGTTCCTGCCACCGTAACGAACCGt                                                                                                        >  1:350256/1‑250 (MQ=255)                                                                                                                                     aaaTTGAAACAATATGACTATCAGGAAGTGAAAGGTCCGTTCATGATGGATCGCGTGTTGTGGGAAAAAACCGGTCACTGGCAAAACTACGGCGATTTGATGTTCACCACCTCTTCTGAAAACCGCGAATATGCCATCAAACCGATGAACTGCCCGGGACACGTTCAGATCTTCAATCAAGGTTTAAAATCTTATCGCGATTTACCGCTACGTATGGCGGAATTCGGTTCCTGCCACCGTAACGAACCGt                                                                                                        >  1:231981/1‑250 (MQ=255)                                                                                                                                                atatGACTATCAGGAAGTGAAAGGTCCGTTCATGATGGATCGCGTGTTGTGGGAAAAAACCGGTCACTGGCAAAACTACGGCGATTTGATGTTCACCACCTCTTCTGAAAACCGCGAATATGCCATCAAACCGATGAACTGCCCGGGACACGTTCAGATCTTCAATCAAAGTTTAAAATCTTATCGCGATTTACCGCTACGTATGGCGGAATTCGGTTCCTGCCACCGTAACGAACCGTCCGGTTCATTg                                                                                             >  1:439895/1‑250 (MQ=255)                                                                                                                                                  atGACTATCAGGAAGTGAAAGGTCCGTTCATGATGGATCGCGTGTTGTGGGAAAAAACCGGTCACTGGCAAAACTACGGCGATTTGATGTTCACCACCTCTTCTGAAAACCGCGAATATGCCATCAAACCGATTAACTGCCCGGGACACGTTCAGATCTTCAATCAAGGTTTAAAATCTTATCGCGATTTACCGCTACGTATGGCGGAATTCGGTTCCTGCCACCGTAACGAACCGTCCGGTTCATTGCAc                                                                                          <  1:616456/251‑1 (MQ=255)                                                                                                                                                    gACTATCAGGAAGTGAAAGGTCCGTTCATGATGGATCGCGTGTTGTGGGAAAAAACCGGTCACTGGCAAAACTACGGCGATTTGATGTTCACCACCTCTTCTGAAAACCGCGAATATGCCATCAAACCGATGAACTGCCCGGGACACGTTCAGATCTTCAATCAAGGTTTAAAATCTTATCGCGATTTACCGCTACGTATGGCGGAATTCGGTTCCTGCCACCGTAACGAACCGTcc                                                                                                      <  1:463350/237‑1 (MQ=255)                                                                                                                                                     aCTATCAGGAAGTGAAAGGTCCGTTCATGATGGATCGCGTGTTGTGGGAAAAAACCGGTCACTGGCAAAACTACGGCGATTTGATGTTCACCACCTCTTCTGAAAACCGCGAATATGCCATCAAACCGATGAACTGCCCGGGACACGTTCAGATCTTCAATCAAGGTTTAAAATCTTATCGCGATTTACCGCTACGTATGGCGGAATTCGGTTCCTGCCACTGTAACGAACCGTCCGGTTCATTGCACGGt                                                                                       >  1:701535/1‑251 (MQ=255)                                                                                                                                                      cTATCAGGAAGTGAAAGGTCCGTTCATGATGGATCGCGTGTTGTGGGAAAAAACCGGTCACTGGCAAAACTACGGCGATTTGATGTTCACCACCTCTTCTGAAAACCGCGAATATGCCATCAAACCGATGAACTGCCCGGGACACGTTCAGATCTTCAATCAAGGTTTAAAATCTTATCGCGATTTACCGCTACGTATGGCGGAATTCGGTTCCTGCCACCGTAACGa                                                                                                             >  1:175697/1‑228 (MQ=255)                                                                                                                                                      cTATCAGGAAGTGAAAGGTCCGTTCATGATGGATCGCGTGTTGTGGGAAAAAACCGGTCACTGGCAAAACTACGGCGATTTGATGTTCACCACCTCTTCTGAAAACCGCGAATATGCCATCAAACCGATGAACTGCCCGGGACACGTTCAGATCTTCAATCAAGGTTTAAAATCTTATCGCGATTTACCGCTACGTATGGCGGAATTCGGTTCCTGCCACCGTAACGAACCGTCCGGTTCATTGCACGGtt                                                                                      >  1:407421/1‑251 (MQ=255)                                                                                                                                                            ggAAGTGAAAGGTCCGTTCATGATGGATCGCGTGTTGTGGGAAAAAACCGGTCACTGGCAAAACTACGGCGATTTGATGTTCACCACCTCTTCTGAAAACCGCGAATATGCCATCAAACCGATGAACTGCCCGGGACACGTTCAGATCTTCAATCAAGGTTTAAAATCTTATCGCGATTTACCGCTACGTATGGCGGAATTCGGTTCCTGCCACCGTAACGAACCGTCCGGTTCATTGCACGGTTTAAtg                                                                                 >  1:152281/1‑250 (MQ=255)                                                                                                                                                             gAAGTGAAAGGTCCGTTCATGATGGATCGCGTGTTGTGGGAAAAAACCGGTCACTGGCAAAACTACGGCGATTTGATGTTCACCACCTCTTCTGAAAACCGCGAATATGCCATCAAACCGATGAACTGCCCGGGACACGTTCAGATCTTCAATCAAGGTTTAAAATCTTATCGCGATTTACCGCTACGTATGGCGGAATTCGGTTCCTGCCACCGTAACGAACCGTCCGGTTCATTGCACGGTTTAAtgcg                                                                               >  1:733998/1‑251 (MQ=255)                                                                                                                                                             gAAGTGAAAGGTCCGTTCATGATGGATCGCGTGTTGTGGGAAAAAACCGGTCACTGGCAAAACTACGGCGATTTGATGTTCACCACCTCTTCTGAAAACCGCGAATATGCCATCAAACCGATGAACTGCCCGGGACACGTTCAGATCTTCAATCAAGGTTTAAAATCTTATCGCGATTTACCGCTACGTATGGCGGAATTCGGTTCCTGCCACCGTAACGAACCGTCCGGTTCATTGCACGGTTTAAtgc                                                                                <  1:210243/250‑1 (MQ=255)                                                                                                                                                             gAAGTGAAAGGTCCGTTCATGATGGATCGCGTGTTGTGGGAAAAAACCGGTCACTGGCAAAACTACGGCGATTTGATGTTCACCACCTCTTCTGAAAACCGCGAATATGCCATCAAACCGATGAACTGCCCGGGACACGTTCAGATCTTCAATCAAGGTTTAAAATCTTATCGCGATTTACCGCTACGTATGGCGGAATTCGGTTCCTGCCACCGTAACGAACCGTCCGGTTCATTGCACGGTTTAAtgc                                                                                <  1:318072/250‑1 (MQ=255)                                                                                                                                                              aaGTGAAAGGTCCGTTCATGATGGATCGCGTGTTGTGGGAAAAAACCGGTCACTGGCAAAACTACGGCGATTTGATGTTCACCACCTCTTCTGAAAACCGCGAATATGCCATCAAACCGATGAACTGCCCGGGACACGTTCAGATCTTCAATCAAGGTTTAAAATCTTATCGCGATTTACCGCTACGTATGGCGGAATTCGGTTCCTGCCACCGTAACGAACCGTCCGGTTCATTGCACGGTTTAAtgcg                                                                               <  1:301790/250‑1 (MQ=255)                                                                                                                                                               aGTGAAAGGTCCGTTCATGATGGATCGCGTGTTGTGGGAAAAAACCGGTCACTGGCAAAACTACGGCGATTTGATGTTCACCACCTCTTCTGAAAACCGCGAATATGCCATCAAACCGATGAACTGCCCGGGACACGTTCAGATCTTCAATCAAGGTTTAAAATCTTATCGCGATTTACCGCTACGTATGGCGGAATTCGGTTCCTGCCACCGTAACGAACCGTCCGGtt                                                                                                  <  1:47216/230‑1 (MQ=255)                                                                                                                                                                gTGAAAGGTCCGTTCATGATGGATCGCGTGTTGTGGGAAAAAACCGGTCACTGGCAAAACTACGGCGATTTGATGTTCACCACCTCTTCTGAAAACCGCGAATATGCCATCAAACCGATGAACTGCCCGGGACACGTTCAGATCTTCAATCAAGGTTTAAAATCTTATCGCGATTTACCGCTACGTATGGCGGAATTCGGTTCCTGCCACCGTAACGAACCGTCCGGTTCATTGCACGGTTTAAtgcgcgt                                                                            >  1:233277/1‑251 (MQ=255)                                                                                                                                                                gTGAAAGGTCCGTTCATGATGGATCGCGTGTTGTGGGAAAAAACCGGTCACTGGCAAAACTACGGCGATTTGATGTTCACCACCTCTTCTGAAAACCGCGAATATGCCATCAAACCGATGAACTGCCCGGGACACGTTCAGATCTTCAATCAAGGTTTAAAATCTTATCGCGATTTACCGCTACGTATGGCGGAATTCGGTTCCTGCCACCGTAACGAACCGTCCGGTTCATTGCACGGTTTAAtgcgcgt                                                                            >  1:116411/1‑251 (MQ=255)                                                                                                                                                                gTGAAAGGTCCGTTCATGATGGATCGCGTGTTGTGGGAAAAAACCGGTCACTGGCAAAACTACGGCGATTTGATGTTCACCACCTCTTCTGAAAACCGCGAATATGCCATCAAACCGATGAACTGCCCGGGACACGTTCAGATCTTCAATCAAGGTTTAAAATCTTATCGCGATTTACCGCTACGTATGGCGGAATTCGGTTCCTGCCACCGTAACGAACCGTCCGGTTCATTGCACGGTTTAAtg                                                                                 >  1:719722/1‑246 (MQ=255)                                                                                                                                                                           gTTCATGATGGATCGCGTGTTGTGGGAAAAAACCGGTCACTGGCAAAACTACGGCGATTTGATGTTCACCACCTCTTCTGAAAACCGCGAATATGCCATCAAACCGATGAACTGCCCGGGACACGTTCAGATCTTCAATCAAGGTTTAAAATCTTATCGCGATTTACCGCTACGTATGGCGGAATTCGGTTCCTGCCACCGTAACGAACCGTCCGGTTCATTGCACGGTTTAAtg                                                                                 >  1:732185/1‑235 (MQ=255)                                                                                                                                                                           gTTCATGATGGATCGCGTGTTGTGGGAAAAAACCGGTCACTGGCAAAACTACGGCGATTTGATGTTCACCACCTCTTCTGAAAACCGCGAATATGCCATCAAACCGATGAACTGCCCGGGACACGTTCAGATCTTCAATCAAGGTTTAAAATCTTATCGCGATTTACCGCTACGTATGGCGGAATTCGGTTCCTGCCACCGTAACGAACCGTCCGGTTCATTGCACGGTTTAATGCGCGTGCGCGGTTTTa                                                                 >  1:852281/1‑251 (MQ=255)                                                                                                                                                                           gTTCATGATGGATCGCGTGTTGTGGGAAAAAACCGGTCACTGGCAAAACTACGGCGATTTGATGTTCACCACCTCTTCTGAAAACCGCGAATATGCCATCAAACCGATGAACTGCCCGGGACACGTTCAGATCTTCAATCAAGGTTTAAAATCTTATCGCGATTTACCGCTACGTATGGCGGAATTCGGTTCCTGCCACCGTAACGAACCGTCCGGTTCATTGCACGGTTTAATGCGCGTGCGCGGTTTTa                                                                 >  1:249984/1‑251 (MQ=255)                                                                                                                                                                                  atgGATCGCGTGTTGTGGGAAAAAACCGGTCACTGGCAAAACTACGGCGATTTGATGTTCACCACCTCTTCTGAAAACCGCGAATATGCCATCAAACCGATGAACTGCCCGGGACACGTTCAGATCTTCAATCAAGGTTTAAAATCTTATCGCGATTTACCGCTACGTATGGCGGAATTCGGTTCCTGCCACCGTAACGAACCGTCCGGTTCATTGCACGGTTTAATGCGCGTGCGCGGTTTTACTCAAGa                                                          >  1:609301/1‑251 (MQ=255)                                                                                                                                                                                      aTCGCGTGTTGTGGGAAAAAACCGGTCACTGGCAAAACTACGGCGATTTGATGTTCACCACCTCTTCTGAAAACCGCGAATATGCCATCAAACCGATGAACTGCCCGGGACACGTTCAGATCTTCAATCAAGGTTTAAAATCTTATCGCGATTTACCGCTACGt                                                                                                                                             <  1:703720/164‑1 (MQ=255)                                                                                                                                                                                      aTCGCGTGTTGTGGGAAAAAACCGGTCACTGGCAAAACTACGGCGATTTGATGTTCACCACCTCTTCTGAAAACCGCGAATATGCCATCAAACCGATGAACTGCCCGGGACACGTTCAGATCTTCAATCAAGGTTTAAAATCTTATCGCGATTTACCGCTACGTATGGCGGAATTCGGTTCCTGCCACCGTAACGAAc                                                                                                           >  1:361621/1‑198 (MQ=255)                                                                                                                                                                                      aTCGCGTGTTGTGGGAAAAAACCGGTCACTGGCAAAACTACGGCGATTTGATGTTCACCACCTCTTCTGAAAACCGCGAATATGCCATCAAACCGATGAACTGCCCGGGACACGTTCAGATCTTCAATCAAGGTTTAAAATCTTATCGCGATTTACCGCTACGTATGGCGGAATTCGGTTCCTGCCACCGTAACGAACCGTCCGGTTCATTGCACGGTTTAATGCGCGTGCGCGGTTTTACTCAAGAt                                                         <  1:17488/248‑1 (MQ=255)                                                                                                                                                                                       tCGCGTGTTGTGGGAAAAAACCGGTCACTGGCAAAACTACGGCGATTTGATGTTCACCACCTCTTCTGAAAACCGCGAATATGCCATCAAACCGATGAACTGCCCGGGac                                                                                                                                                                                                  <  1:891238/110‑1 (MQ=255)                                                                                                                                                                                                  gggAAAAAACCGGTCACTGGCAAAACTACGGCGATTTGATGTTCACCACCTCTTCTGAAAACCGCGAATATGCCATCAAACCGATGAACTGCCCGGGACACGTTCAGATCTTCAATCAAGGTTTAAAATCTTATCGCGATTTACCGCTACGTATGGCGGAATTCGGTTCCTGCCACCGTAACGAACCGTCCGGTTCATTGCACGGTTTAATGCGCGTGCGCGGTTTTACTCAAGATGACGCTCACAtttt                                           >  1:178601/1‑250 (MQ=255)                                                                                                                                                                                                     aaaaaaCCGGTCACTGGCAAAACTACGGCGATTTGATGTTCACCACCTCTTCTGAAAACCGCGAATATGCCATCAAACCGATGAACTGCCCGGGACACGTTCAGATCTTCAATCAAGGTTTAAAATCTTATCGCGATTTACCGCTACGTATGGCGGAATTCGGTTCCTGCCACCGTAACGAACCGTCCGGTTCATTGCACGGTTTAATGCGCGTGCGCGGTTTTACTCAAGATGACGCTCACATTTTCTg                                        <  1:267383/250‑1 (MQ=255)                                                                                                                                                                                                          aCCGGTCACTGGCAAAACTACGGCGATTTGATGTTCACCACCTCTTCTGAAAACCGCGAATATGCCATCAAACCGATGAACTGCCCGGGACACGTTCAGATCTTCAATCAAGGTTTAAAATCTTATCGCGATTTACCGCTACGTATGGCGGAATTCGGTTCCTGCCACCGTAACGAACCGTCCGGTTCATTGCACGGTTTAATGCGCGTGCGCGGTTTTACTCAAGATGACGCTCACATTTTCTGTATGcc                                  <  1:83515/251‑1 (MQ=255)                                                                                                                                                                                                            cGGTCACTGGCAAAACTACGGCGATTTGATGTTCACCACCTCTTCTGAAAACCGCGAATATGCCATCAAACCGATGAACTGCCCGGGACACGTTCAGATCTTCAATCAAGGTTTAAAATCTTATCGCGATTTACCGCTACGTATGGCGGAATTCGGTTCCTGCCACCGTAACGAACCGTCCGGTTCATTGCACGGTTTAATGCGCGTGCGCGGTTTTACTCAAGATGACGCTCACATTTTCTGTATGCCgg                                <  1:209206/251‑1 (MQ=255)                                                                                                                                                                                                                    ggCAAAACTACGGCGATTTGATGTTCACCACCTCTTCTGAAAACCGCGAATATGCCATCAAACCGATGAACTGCCCGGGACACGTTCAGATCTTCAATCAAGGTTTAAAATCTTATCGCGATTTACCGCTACGTATGGCGGAATTCGGTTCCTGCCACCGTAACGAACCGTCCGGTTCATTGCACGGTTTAAtgc                                                                                <  1:800085/195‑1 (MQ=255)                                                                                                                                                                                                                    ggCAAAACTACGGCGATTTGATGTTCACCACCTCTTCTGAAAACCGCGAATATGCCATCAAACCGATGAACTGCCCGGGACACGTTCAGATCTTCAATCAAGGTTTAAAATCTTATCGCGATTTACCGCTACGTATGGCGGAATTCGGTTCCTGCCACCGTAACGAACCGTCCGGTTCATTGCACGGTTTAATGCGCGTGCGCGGTTTTACTCAAGATGACGCTCACATTTTCTGTATGCCGGATCAAGTg                        >  1:280615/1‑251 (MQ=255)                                                                                                                                                                                                                                 cGATTTGATGTTCACCACCTCTTCTGAAAACCGCGAATATGCCATCAAACCGATGAACTGCCCGGGACACGTTCAGATCTTCAATCAAGGTTTAAAATCTTATCGCGATTTACCGCTACGTATGGCGGAATTCGGTTCCTGCCACCGTGACGAACCGTCCGGTTCATTGCACGGTTTAATGCGCGTGCGCGGTTTTACTC‑AGATGACGCTCACATTTTCTGTATGCCGGATCAAGTGGAAAGCGAAGTGa           <  1:462976/250‑1 (MQ=255)                                                                                                                                                                                                                                  gATTTGATGTTCACCACCTCTTCTGAAAACCGCGAATATGCCATCAAACCGATGAACTGCCCGGGACACGTTCAGATCTTCAATCAAGGTTTAAAATCTTATCGCGATTTACCGCTACGTATGGCGGAATTCGGTTCCTGCCACCGTAACGAACCGTCCGGTTCATTGCACGGTTTAATGCGCGTGCGCGGTTTTACTCAAGATGACGCTCACATTTTCTGTATGCCGGATCAAGTGGAAAGCGAAGTGa           >  1:720889/1‑250 (MQ=255)                                                                                                                                                                                                                                          gTTCACCACCTCTTCTGAAAACCGCGAATATGCCATCAAACCGATGAACTGCCCGGGACACGTTCAGATCTTCAATCAAGGTTTAAAATCTTATCGCGATTTACCGCTACGTATGGCGGAATTCGGTTCCTGCCACCGTAACGAACCGTCCGGTTCATTGCACGGTTTAATGCGCGTGCGCGGTTTTACTCAAGATGACGCTCACATTTTCTGTATGCCGGATCAAGTGGAAAGCGAAGTGACTTCCTGCa  >  1:32355/1‑251 (MQ=255)                                                                                                                                                                                                                                            tCACCACCTCTTCTGAAAACCGCGAATATGCCATCAAACCGATGAACTGCCCGGGACACGTTCAGATCTTCAATCAAGGTTTAAAATCTTATCGCGATTTACCGCTACGTATGGCGGAATTCGGTTCCTGCCACCGTAACGAACCGTCCGGTTCATTGCACGGTTTAAtg                                                                                 >  1:647267/1‑170 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                GCAAAACGGGATCATCGTAAAATCGGTAAAGCATTGGATTTATATCACATGCAGGAAGAAGCGCCGGGTATGGTGTTCTGGCACAATGACGGCTGGACGATTTTCCGTGAACTGGAAACTTTCGTGCGCACTAAATTGAAACAATATGACTATCAGGAAGTGAAAGGTCCGTTCATGATGGATCGCGTGTTGTGGGAAAAAACCGGTCACTGGCAAAACTACGGCGATTTGATGTTCACCACCTTTTCTGAAAACCGCGAATATGCCATCAAACCGATGAACTGCCCGGGACACGTTCAGATCTTCAATCAAGGTTTAAAATCTTATCGCGATTTACCGCTACGTATGGCGGAATTCGGTTCCTGCCACCGTAACGAACCGTCCGGTTCATTGCACGGTTTAATGCGCGTGCGCGGTTTTACTCAAGATGACGCTCACATTTTCTGTATGCCGGATCAAGTGGAAAGCGAAGTGACTTCCTGCA  >  1/1229458‑1229941

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑