Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,226,345	A→C	intergenic (+87215/+166966)	unknown → / ← unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,226,345	0	A	C	100.0%	185.2 / NA	65	intergenic (+87215/+166966)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (36/29);  total (36/29)

TCGGTGTGGTAGCGCAGATTCCAAATGTTCATCGCCTACTAAAAAAACATGATGTGGATGTGGACGTGATGACGGCAGGCGAATATAAACGCACTATGACGATTTTTGGTGAAAATACCGAAAAAGGAAAGCAAAAATTCCAACAGGAATTAGAAGAAACTCATCAGTTATTCAAGCAATTTGTCGCACAGAATCGACCGCACTTGGATCTGGATAAAGTAGCAACAGGGGAACATTGGTTAGGTCAGCAGGCATTAAATTTAAATTTAGTGGATGAGATTATGACCAGCGATGATTTGCTATTACAGGCGATGAAAGAGAAATCGGTTATCGGCGTAAAATATGCGGTGAAGAAATCGTTATTACAAAAAGTCGGTAAACAGGCGGAAGAAAGTGCCGATAATATTGCTTTACGTTGGCTTAAGAAAAATGAAAGAACATTGATATAACATCCTATTTTTTTCGATTCTATTTATAC  >  1/1226104‑1226581                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                             tCGGTGTGGTAGCGCAGATTCCAAATGTTCATCGCCTACTAAAAAAACATGATGTGGATGTGGACGTGATGACGGCAGGCGAATATAAACGCACTATGACGATTTTTGGTGAAAATACCGAAAAAGGAAAGCAAAAATTCCAACAGGAATTAGAAGAAACTCATCAGTTATTCAAGCAATTTGTCGCACAGAATCGACCGCACTTGGATCTGGATAAAGTAGCAACAGGGGAACATTGGTTCGGTcagcag                                                                                                                                                                                                                                     <  1:552117/251‑1 (MQ=255)    gtgtGGTAGCGCAGATTCCAAATGTTCATCGCCTACTAAAAAAACATGATGTGGATGTGGACGTGATGACGGCAGGCGAATATAAACGCACTATGACGATTTTTGGTGAAAATACCGAAAAAGGAAAGCAAAAATTCCAACAGGAATTAGAAGAAACTCATCAGTTATTCAAGCAATTTGTCGCACAGAATCGACCGCACTTGGATCTGGATAAAGTAGCAACAGGGGAACATTGGTTCGGTCAGCAGGCa                                                                                                                                                                                                                                  >  1:587106/1‑251 (MQ=255)    gtgtGGTAGCGCAGATTCCAAATGTTCATCGCCTACTAAAAAAACATGATGTGGATGTGGACGTGATGACGGCAGGCGAATATAAACGCACTATGACGATTTTTGGTGAAAATACCGAAAAAGGAAAGCAAAAATTCCAACAGGAATTAGAAGAAACTCATCAGTTATTCAAGCAATTTGTCGCACAGAATCGACCGCACTTGGATCTGGATAAAGTAGCAACAGGGGAACATTGGTTCGGTCAGCAGGCa                                                                                                                                                                                                                                  >  1:197303/1‑251 (MQ=255)    gtgtGGTAGCGCAGATTCCAAATGTTCATCGCCTACTAAAAAAACATGATGTGGATGTGGACGTGATGACGGCAGGCGAATATAAACGCACTATGACGATTTTTGGTGAAAATACCGAAAAAGGAAAGCAAAAATTCCAACAGGAATTAGAAGAAACTCATCAGTTATTCAAGCAATTTGTCGCACAGAATCGACCGCACTTGGATCTGGATAAAGTAGCAACAGGGGAACATTGGTTCGGTCAGCAGGCa                                                                                                                                                                                                                                  >  1:207950/1‑251 (MQ=255)    gtgtGGTAGCGCAGATTCCAAATGTTCATCGCCTACTAAAAAAACATGATGTGGATGTGGACGTGATGACGGCAGGCGAATATAAACGCACTATGACGATTTTTGGTGAAAATACCGAAAAAGGAAAGCAAAAATTCCAACAGGAATTAGAAGAAACTCATCAGTTATTCAAGCAATTTGTCGCACAGAATCGACCGCACTTGGATCTGGATAAAGTAGCAACAGGGGAACATTGGTTCGGTCAGCAGGCa                                                                                                                                                                                                                                  >  1:872217/1‑251 (MQ=255)       tgGTAGCGCAGATTCCAAATGTTCATCGCCTACTAAAAAAACATGATGTGGATGTGGACGTGATGACGGCAGGCGAATATAAACGCACTATGACGATTTTTGGTGAAAATACCGAAAAAGGAAAGCAAAAATTCCAACAGGAATTAGAAGAAACTCATCAGTTATTCAAGCAATTTGTCGCACAGAATCGACCGCACTTGGATCTGGATAAAGTAGCAACAGGGGAACATTGGTTCGGTCAGCAGGCAtta                                                                                                                                                                                                                               >  1:569592/1‑251 (MQ=255)                  aTTCCAAATGTTCATCGCCTACTAAAAAAACATGATGTGGATGTGGACGTGATGACGGCAGGCGAATATAAACGCACTATGACGATTTTTGGTGAAAATACCGAAAAAGGAAAGCAAAAATTCCAACAGGAATTAGAAGAAACTCATCAGTTATTCAAGCAATTTGTCGCACAGAATCGACCGCACTTGGATCTGGATAAAGTAGCAACAGGGGAACATTGGTTCGGTCAGCAGGCATtaaatttaaatt                                                                                                                                                                                                                     <  1:863486/250‑1 (MQ=255)                   ttCCAAATGTTCATCGCCTACTAAAAAAACATGATGTGTATGTGGACGTGATGACGGCAGGCGAATATAAACGCACTATGACGATTTTTGGTGAAAATACCGAAAAAGGAAAGCAAAAATTCCAACAGGAATTAGAAGAAACTCATCAGTTATTCAAGCAATTTGTCGCACAGAATCGACCGCACTTGGATCTGGATAAAGTAGCAACAGGGGAACATTGGTTCGGTCAGCAGGCATTAaatttaaattta                                                                                                                                                                                                                   >  1:180949/1‑251 (MQ=255)                   ttCCAAATGTTCATCGCCTACTAAAAAAACATGATGTGGATGTGGACGTGATGACGGCAGGCGAATATAAACGCACTATGACGATTTTTGGTGAAAATACCGAAAAAGGAAAGCAAAAATTCCAACAGGAATTAGAAGAAACTCATCAGTTATTCAAGCAATTTGTCGCACAGAATCGACCGCACTTGGATCTGGATAAAGTAGCAACAGGGGAACATTGGTTCGGTCAGCAGGCATTAaatttaaattta                                                                                                                                                                                                                   >  1:664583/1‑251 (MQ=255)                   ttCCAAATGTTCATCGCCTACTAAAAAAACATGATGTGGATGTGGACGTGATGACGGCAGGCGAATATAAACGCACTATGACGATTTTTGGTGAAAATACCGAAAAAGGAAAGCAAAAATTCCAACAGGAATTAGAAGAAACTCATCAGTTATTCAAGCAATTTGTCGCACAGAATCGACCGCACTTGGATCTGGATAAAGTAGCAACAGGGGAACATTGGTTCGGTCAGCAGGCATTAaatttaaattta                                                                                                                                                                                                                   >  1:386967/1‑251 (MQ=255)                           gTTCATCGCCTACTAAAAAAACATGATGTGGATGTGGACGTGATGACGGCAGGCGAATATAAACGCACTATGACGATTTTTGGTGAAAATACCGAAAAAGGAAAGCAAAAATTCCAACAGGAATTAGAAGAAACTCATCAGTTATTCAAGCAATTTGTCGCACAGAATCGACCGCACTTGGATCTGGATAAAGTAGCAACAGGGGAACATTGGTTCGGTCAGCAGGCATTAAATTTAAATTTAGTGGATga                                                                                                                                                                                                           >  1:899969/1‑251 (MQ=255)                                    ctactaAAAAAACATGATGTGGATGTGGACGTGATGACGGCAGGCGAATATAAACGCACTATGACGATTTTTGGTGAAAATACCGAAAAAGGAAAGCAAAAATTCCAACAGGAATTAGAAGAAACTCATCAGTTATTCAAGCAATTTGTTGCACAGAATCGACCGCACTTGGATCTGGATAAAGTAGCAACAGGGGAACATTGGTTCGGTCAGCAGGCATTAAATTTAAATTTAGTGGATGAGATTATGAc                                                                                                                                                                                                  <  1:8337/251‑1 (MQ=255)                                    ctactaAAAAAACATGATGTGGATGTGGACGTGATGACGGCAGGCGAATATAAACGCACTATGACGATTTTTGGTGAAAATACCGAAAAAGGAAAGCAAAAATTCCAACAGGAATTAGAAGAAACTCATCAGTTATTCAAGCAATTTGTCGCACAGAATCGACCGCACTTGGATCTGGATAAAGTAGCAACAGGGGAACATTGGTTCGGTCAGCAGGCATTAAATTTAAATTTAGTGGATGAGATTATGAc                                                                                                                                                                                                  <  1:651342/251‑1 (MQ=255)                                    ctactaAAAAAACATGATGTGGATGTGGACGTGATGACGGCAGGCGAATATAAACGCACTATGACGATTTTTGGTGAAAATACCGAAAAAGGAAAGCAAAAATTCCAACAGGAATTAGAAGAAACTCATCAGTTATTCAAGCAATTTGTCGCACAGAATCGACCGCACTTGGATCTGGATAAAGTAGCAACAGGGGAACATTGGTTCGGTCAGCAGGCATTAAATTTAAATTTAGTGGATGAGATTATGAc                                                                                                                                                                                                  <  1:864404/251‑1 (MQ=255)                                    ctactaAAAAAACATGATGTGGATGTGGACGTGATGACGGCAGGCGAATATAAACGCACTATGACGATTTTTGGTGAAAATACCGAAAAAGGAAAGCAAAAATTCCAACAGGAATTAGAAGAAACTCATCAGTTATTCAAGCAATTTGTCGCACAGAATCGACCGCACTTGGATCTGGATAAAGTAGCAACAGGGGAACATTGGTTCGGTCAGCAGGCATTAAATTTAAATTTAGTGGATGAGATTATGAc                                                                                                                                                                                                  <  1:654714/251‑1 (MQ=255)                                    ctactaAAAAAACATGATGTGGATGTGGACGTGATGACGGCAGGCGAATATAAACGCACTATGACGATTTTTGGTGAAAATACCGAAAAAGGAAAGCAAAAATTCCAACAGGAATTAGAAGAAACTCATCAGTTATTCAAGCAATTTGTCGCACAGAATCGACCGCACTTGGATCTGGATAAAGTAGCAACAGGGGAACATTGGTTCGGTCAGCAGGCATTAAATTTAAATTTAGTGGATGAGATTATGAc                                                                                                                                                                                                  <  1:94975/251‑1 (MQ=255)                                     tactaAAAAAACATGATGTGGATGTGGACGTGATGACGGCAGGCGAATATAAACGCACTATGACGATTTTTGGTGAAAATACCGAAAAAGGAAAGCAAAAATTCCAACAGGAATTAGAAGAAACTCATCAGTTATTCAAGCAATTTGTCGCACAGAATCGACCGCACTTGGATCTGGATAAAGTAGCAACAGGGGAACATTGGTTCGGTCAGCAGGCATTAAATTTAAATTTAGTGGATGAGATTATGAcc                                                                                                                                                                                                 <  1:544500/251‑1 (MQ=255)                                                    atgtggatgtggACGTGATGACGGCAGGCGAATATAAACGCACTATGACGATTTTTGGTGAAAATACCGAAAAAGGAAAGCAAAAATTCCAACAGGAATTAGAAGAAACTCATCAGTTATTCAAGCAATTTGTCGCACAGAATCGACCGCACTTGGATCTGGATAAAGTAGCAACAGGGGAACATTGGTTCGGTCAGCAGGCATTAAATTTAAATTTAGTGGATGAGATTATGACCAGCGATGATTTGCTa                                                                                                                                                                                  >  1:487317/1‑251 (MQ=255)                                                      gtggatgtggaCGTGATGACGGCAGGCGAATATAAACGCACTATGACGATTTTTGGTGAAAATACCGAAAAAGGAAAGCAAAAATTCCAACAGGAATTAGAAGAAACTCATCAGTTATTCAAGCAATTTGTCGCACAGAATCGACCGCACTTGGATCTGGATAAAGTAGCAACAGGGGAACATTGGTTCGGTCAGCAGGCATTAAATTTAAATTTAGTGGATGAGATTATGACCAGCGATGATTTGCTAtt                                                                                                                                                                                >  1:83473/1‑251 (MQ=255)                                                        ggatgtggaCGTGATGACGGCAGGCGAATATAAACGCACTATGACGATTTTTGGTGAAAATACCGAAAAAGGAAAGCAAAAATTCCAACAGGAATTAGAAGAAACTCATCAGTTATTCAAGCAATTTGTCGCACAGAATCGACCGCACTTGGATCTGGATAAAGTAGCAACAGGGGAACATTGGTTCGGTCAGCAGGCATTAAATTTAAATTTAGTGGATGAGATTATGACCAGCGATGATTTGCTATTa                                                                                                                                                                               >  1:580577/1‑250 (MQ=255)                                                         gatgtggaCGTGATGACGGCAGGCGAATATAAACGCACTATGACGATTTTTGGTGAAAATACCGAAAAAGGAAAGCAAAAATTCCAACAGGAATTAGAAGAAACTCATCAGTTATTCAAGCAATTTGTCGCACAGAATCGACCGCACTTGGATCTGGATAAAGTAGCAACAGGGGAACATTGGTTCGGTCAGCAGGCATTAAATTTAAATTTAg                                                                                                                                                                                                                  <  1:96918/214‑1 (MQ=255)                                                         gatgtggaCGTGATGACGGCAGGCGAATATAAACGCACTATGACGATTTTTGGTGAAAATACCGAAAAAGGAAAGCAAAAATTCCAACAGGAATTAGAAGAAACTCATCAGTTATTCAAGCAATTTGTCGCACAGAATCGACCGCACTTGGATCTGGATAAAGTAGCAACAGGGGAACATTGGTTCGGTCAGCAGGCATTAAATTTAAATTTAGTGGATGAGATTATGACCAGCGATGATTTGCTATTAc                                                                                                                                                                              <  1:895518/250‑1 (MQ=255)                                                           tgtggaCGTGATGACGGCAGGCGAATATAAACGCACTATGACGATTTTTGGTGAAAATACCGAAAAAGGAAAGCAAAAATTCCAACAGGAATTAGAAGAAACTCATCAGTTATTCAAGCAATTTGTCGCACAGAATCGACCGCACTTGGATCTGGATAAAGTAGCAACAGGGGAACATTGGTTCGGTCAGCAGGCATTAAATTTAAATTTAGTGGATGAGATTATGACCAGCGATGATTTGCTATTACAgg                                                                                                                                                                           <  1:78590/251‑1 (MQ=255)                                                               gaCGTGATGACGGCAGGCGAATATAAACGCACTATGACGATTTTTGGTGAAAATACCGAAAAAGGAAAGCAAAAATTCCAACAGGAATTAGAAGAAACTCATCAGTTATTCAAGCAATTTGTCGCACAGAATCGACCGCACTTGGATCTGGATAAAGTAGCAACAGGGGAACATTGGTTCGGTCAGCAGGCATTAAATTTAAATTTAGTGGATGAGATTATGAcc                                                                                                                                                                                                 <  1:271939/225‑1 (MQ=255)                                                                     atgaCGGCAGGCGAATATAAACGCACTATGACGATTTTTGGTGAAAATACCGAAAAAGGAAAGCAAAAATTCCAACAGGAATTAGAAGAAACTCATCAGTTATTCAAGCAATTTGTCGCACAGAATCGACCGCACTTGGATCTGGATAAAGTAGCAACAGGGGAACATTGGTTCGGTCAGCAGGCATTAAATTTAAATTTAGTGGATGAGATTATGACCAGCGATGATTTGCTATTACAGGCGATGAAag                                                                                                                                                                  <  1:800080/250‑1 (MQ=255)                                                                         cGGCAGGCGAATATAAACGCACTATGACGATTTTTGGTGAAAATACCGAAAAAGGAAAGCAAAAATTCCAACAGGAATTAGAAGAAACTCATCAGTTATTCAAGCAATTTGTCGCACAGAATCGACCGCACTTGGATCTGGATAAAGTAGCAACAGGGGAACATTGGTTCGGTCAGCAGGCATTAAATTTAAATTTAGTGGATGAGATTATGACCAGCGATGATTTGCTATTACAGGCGATGAAAGAGaaa                                                                                                                                                             >  1:753170/1‑251 (MQ=255)                                                                                 gAATATAAACGCACTATGACGATTTTTGGTGAAAATACCGAAAAAGGAAAGCAAAAATTCCAACAGGAATTAGAAGAAACTCATCAGTTATTCAAGCAATTTGTCGCACAGAATCGACCGCACTTGGATCTGGATAAAGTAGCAACAGGGGAACATTGGTTCGGTCAGCAGGCAttaaa                                                                                                                                                                                                                             >  1:228974/1‑179 (MQ=255)                                                                                  aaTATAAACGCACTATGACGATTTTTGGTGAAAATACCGAAAAAGGAAAGCAAAAATTCCAACAGGAATTAGAAGAAACTCATCAGTTATTCAAGCAATTTGTCGCACAGAATCGACCGCACTTGGATCTGGATAAAGTAGCAACAGGGGAACATTGGTTCGGTCAGCAGGCATTAAATTTAAATTTAGTGGATGAGATTATGACCAGCGATGATTTGCTATTACAGGCGATGAAAGAGAAATCGGTTAt                                                                                                                                                     >  1:715607/1‑250 (MQ=255)                                                                                    tataAACGCACTATGACGATTTTTGGTGAAAATACCGAAAAAGGAAAGCAAAAATTCCAACAGGAATTAGAAGAAACTCATCAGTTATTCAAGCAATTTGTCGCACAGAATCGACCGCACTTGGATCTGGATAAAGTAGCAACAGGGGAACATTGGTTCGGTCAGCAGGCATTAAATTTAAATTTAGTGGATGAGATTATGACCAGCGATGATTTGCTATTACAGGCGATGaa                                                                                                                                                                    >  1:617868/1‑233 (MQ=255)                                                                                    tataAACGCACTATGACGATTTTTGGTGAAAATACCGAAAAAGGAAAGCAAAAATTCCAACAGGAATTAGAAGAAACTCATCAGTTATTCAAGCAATTTGTCGCACAGAATCGACCGCACTTGGATCTGGATAAAGTAGCAACAGGGGAACATTGGTTCGGTCAGCAGGCATTAAATTTAAATTTAGTGGATGAGATTATGACCAGCGATGATTTGCTATTACAGGCGATGAAAGAGAAATCGGTTATCgg                                                                                                                                                  >  1:704930/1‑251 (MQ=255)                                                                                      taAACGCACTATGACGATTTTTGGTGAAAATACCGAAAAAGGAAAGCAAAAATTCCAACAGGAATTAGAAGAAACTCATCAGTTATTCAAGCAATTTGTCGCACAGAATCGACCGCACTTGGATCTGGATAAAGTAGCAACAGGGGAACATTGGTTCGGTCAGCAGGCATTAAATTTAAATTTAGTGGATGAGATTATGACCAGCGATGATTTGCTATTACAGGCGATGAAAGAGAAATCGGTTATCGGCg                                                                                                                                                >  1:866917/1‑251 (MQ=255)                                                                                            cACTATGACGATTTTTGGTGAAAATACCGAAAAAGGAAAGCAAAAATTCCAACAGGAATTAGAAGAAACTCATCAGTTATTCAAGCAATTTGTCGCACAGAATCGACCGCACTTGGATCTGGATAAAGTAGCAACAGGGGAACATTGGTTCGGTCAGCAGGCATTAAATTTAAATTTAGTGGATGAGAt                                                                                                                                                                                                        <  1:459524/189‑1 (MQ=255)                                                                                              cTATGACGATTTTTGGTGAAAATACCGAAAAAGGAAAGCAAAAATTCCAACAGGAATTAGAAGAAACTCATCAGTTATTCAAGCAATTTGTCGCACAGAATCGACCGCACTTGGATCTGGATAAAGTAGCAACAGGGGAACATTGGTTCGGTCAGCAGGCATTAAATTTAAATTTAGTGGATGAGATTATGACCAGCGATGATTTGCTATTACAGGCGATGAAAGAGAAATCGGTTATCGGCGTAAAatat                                                                                                                                        >  1:559755/1‑251 (MQ=255)                                                                                              cTATGACGATTTTTGGTGAAAATACCGAAAAAGGAAAGCAAAAATTCCAACAGGAATTAGAAGAAACTCATCAGTTATTCAAGCAATTTGTCGCACAGAATCGACCGCACTTGGATCTGGATAAAGTAGCAACAGGGGAACATTGGTTCGGTCAGCAGGCATTAAATTTAAA‑TTAGTGGATGAGATTATGACCAGCGATGAtt                                                                                                                                                                                       >  1:740853/1‑203 (MQ=255)                                                                                                                                    cAAAAATTCCAACAGGAATTAGAAGAAACTCATCAGTTATTCAAGCAATTTGTCGCACAGAATCGACCGCACTTGGATCTGGATAAAGTAGCAACAGGGGAACATTGGTTCGGTCAGCAGGCATTAAATTTAAATTTAGTGGATGAGATTATGACCAGCGATGATTTGCTATTACAGGCGATGAAAGAGAAATCGGTTATCGGCGTaaaa                                                                                                                                           <  1:401539/210‑1 (MQ=255)                                                                                                                                                  ggAATTAGAAGAAACTCATCAGTTATTCAAGCAATTTGTCGCACAGAATCGACCGCACTTGGATCTGGATAAAGTAGCAACAGGGGAACATTGGTTCGGTCAGCAGGCATTAAATTTAAATTTAGTGGATGAGATTATGAc                                                                                                                                                                                                  <  1:192696/141‑1 (MQ=255)                                                                                                                                                    aaTTAGAAGAAACTCATCAGTTATTCAAGCAATTTGTCGCACAGAATCGACCGCACTTGGATCTGGATAAAGTAGCAACAGGGGAACATTGGTTCGGTCAGCAGGCATTAAATTTAAATTTAGTGGATGAGATTATGACCAGCGATGATTTGCTATTACAGGCGATGAAAGAGAAATCGGTTATCGGCGTAAAATATGCGGTGAAGAAATCGTTATTACAAAAAGTCGGTAAACAGGCGGAAGAAAGTGc                                                                                   >  1:141278/1‑250 (MQ=255)                                                                                                                                                          aagaaACTCATCAGTTATTCAAGCAATTTGTCGCACAGAATCGACCGCACTTGGATCTGGATAAAGTAGCAACAGGGGAACATTGGTTCGGTCAGCAGGCATTAAATTTAAATTTAGTGGATGAGATTATGACCAGCGATGATTTGCTATTACAGGCGATGAAAGAGAAATCGGTTATCGGCGTAAAATATGCGGTGAAGAAATCGTTATTACAAAAAGTCGGTAAACAGGCGGAAGAAAGTGCCGataat                                                                            <  1:10161/251‑1 (MQ=255)                                                                                                                                                          aagaaACGCATCAGTTATTCAAGCAATTTGTCGCACAGAATCGACCGCACTTGGATCTGGATAAAGTAGCAACAGGGGAACATTGGTTCGGTCAGCAGGCATTAAATTTAAATTTAGTGGATGAGATTATGACCAGCGATGATTTGCTATTACAGGCGATGAAAGAGAAATCGGATATCGGCGTAAAATATGCGGTGAAGAAATCGTTATTACAAAAAGTCGGTAAACAGGCGGAAGAAAGTGCCGataat                                                                            <  1:33988/251‑1 (MQ=255)                                                                                                                                                                cTCATCAGTTATTCAAGCAATTTGTCGCACAGAATCGACCGCACTTGGATCTGGATAAAGTAGCAACAGGGGAACATTGGTTCGGTCAGCAGGCATTAAATTTAAATTTAGTGGATGAGATTATGACCAGCGATGATTTGCTATTACAGGCGATGAAAGAGAAATCGGTTATCGGCGTAAAata                                                                                                                                         >  1:786722/1‑184 (MQ=255)                                                                                                                                                                 tcatcaGTTATTCAAGCAATTTGTCGCACAGAATCGACCGCACTTGGATCTGGATAAAGTAGCAACAGGGGAACATTGGTTCGGTCAGCAGGCATTAAATTTAAATTTAGTGGATGAGATTATGACCAGCGATGATTTGCTATTACAGGCGATGAAAGAGAAATCGGTTATCGGCGTAAAATATGCGGTGAAGAAATCGTTATTACAAAAAGTCGGTAAACAGGCGGAAGAAAGTGCCGATAATATTGCtt                                                                     <  1:122998/251‑1 (MQ=255)                                                                                                                                                                     caGTTATTCAAGCAATTTGTCGCACAGAATCGACCGCACTTGGATCTGGATAAAGTAGCAACAGGGGAACATTGGTTCGGTCAGCAGGCATTAAATTTAAATTTAGTGGATGAGATTATGACCAGCGATGATTTGCTATTACAGGCGATGAAAGAGAAATCGGt                                                                                                                                                        >  1:832265/1‑164 (MQ=255)                                                                                                                                                                        ttATTCAAGCAATTTGTCGCACAGAATCGACCGCACTTGGATCTGGATAAAGTAGCAACAGGGGAACATTGGTTCGGTCAGCAGGCATTAAATTTAAATTTAGTGGATGAGATTATGACCAGCGATGATTTGCTATTACAGGCGATGAAAGAGAAATCGGTTATCGGCGTAAAATATGCGGTGAAGAAATCGTTATTACAAAAAGTCGGTAAACAGGCGGAAGAAAGTGCCGATAATATTGCTTTACGt                                                                <  1:402731/249‑1 (MQ=255)                                                                                                                                                                        ttATTCAAGCAATTTGTCGCACAGAATCGACCGCACTTGGATCTGGATAAAGTAGCAACAGGGGAACATTGGTTCGGTCAGCAGGCATTAAATTTAAATTTAGTGGATGAGATTATGACCAGCGATGATTTGCTATTACAGGCGATGAAAGAGAAATCGGTTATCGGCGTAAAATATGCGGTGAAGAAATCGTTATTACAAAAAGTCGGTAAACAGGCGGAAGAAAGTGCCGATAATATTGCTTTACGt                                                                <  1:529434/249‑1 (MQ=255)                                                                                                                                                                                     ttGTCGCACAGAATCGACCGCACTTGGATCTGGATAAAGTAGCAACAGGGGAACATTGGTTCGGTCAGCAGGCATTAAATTTAAATTTAGTGGATGAGATTATGACCAGCGATGATTTGCTATTACAGGCGATGAAAGAGAAATCGGTTATCGGCGTAAAATATGCGGTGAAGAAATCGTTATTACAAAAAGTCGGTAAACAGGCGGAAGAAAGTGCCGATAATATTGCTTTACGTTGGCTTAAGAAAAAt                                                 >  1:711023/1‑251 (MQ=255)                                                                                                                                                                                      tGTCGCACAGAATCGACCGCACTTGGATCTGGATAAAGTAGCAACAGGGGAACATTGGTTCGGTCAGCAGGCATTAAATTTAAATTTAGTGGATGAGATTATGACCAGCGATGATTTGCTATTACAGGCGATGAAAGAGAAATCGGATATCGGCGTAAAATATGCGGTGAAGAAATCGTTATTACAAAAAGTCGGTAAACAGGCGgaagaa                                                                                        <  1:63279/211‑1 (MQ=255)                                                                                                                                                                                               gAATCGACCGCACTTGGATCTGGATAAAGTAGCAACAGGGGAACATTGGTTCGGTCAGCAGGCATTAAATTTAAATTTAGTGGATGAGATTATGACCAGCGATGATTTGCTATTACAGGCGATGAAAGAGAAATCGGTTATCGGCGTAAAATATGCGGTGAAGAAATCGTTATTACAAAAAGTCGGTAAACAGGCGGAAGAAAGTGCCGATAATATTGCTTTACGTTGGCTTAAGAAAAATGAAAGAACAt                                       >  1:799470/1‑251 (MQ=255)                                                                                                                                                                                                     aCCGCACTTGGATCTGGATAAAGTAGCAACAGGGGAACATTGGTTCGGTCAGCAGGCATTAAATTTAAATTTAGTGGATGAGATTATGACCAGCGATGATTTGCTATTACAGGCGATGAAAGAGAAATCGGTTATCGGCGTAAAATATGCGGTGAAGAAATCGTTATTACAAAAAGTCGGTAAACAGGCGGAAGAAAGTGCCGATAATATTGCTTTACGTTGGCTTAAGAAAAATGAAAGAACATTGatat                                 <  1:499529/251‑1 (MQ=255)                                                                                                                                                                                                     aCCGCACTTGGATCTGGATAAAGTAGCAACAGGGGAACATTGGTTCGGTCAGCAGGCATTAAATTTAAATTTAGTGGATGAGATTATGACCAGCGATGATTTGCTATTACAGGCGATGAAAGAGAAATCGGTTATCGGCGTAAAATATGCGGTGAAGAAATCGTTATTACAAAAAGTCGGTAAACAGGCGGAAGAAAGTGCCGATAATATTGCTTTACGTTGGCTTAAGAAAAATGAAAGAACATTGata                                  <  1:883947/250‑1 (MQ=255)                                                                                                                                                                                                         cACTTGGATCTGGATAAAGTAGCAACAGGGGAACATTGGTTCGGTCAGCAGGCATTAAATTTAAATTTAGTGGATGAGATTATGACCAGCGATGATTTGCTATTACAGGCGATGAAAGAGAAATCGGTTATCGGCGTAAAATATGCGGTGAAGAAATCGTTATTACAAAAAGTCGGTAAACAGGCGGAAGAAAGTGCCGATAATATTGCTTTACGTTGGCTTAAGAAAAATGAAAGAACATTGATATAAc                              <  1:202342/250‑1 (MQ=255)                                                                                                                                                                                                          aCTTGGATCTGGATAAAGTAGCAACAGGGGAACATTGGTTCGGTCAGCAGGCATTAAATTTAAATTTAGTGGATGAGATTATGACCAGCGATGATTTGCTATTACAGGCGATGAAAGAGAAATCGGTTATCGGCGTAAAATATGCGGTGAAGAAATCGTTATTACAAAAAGTCGGTAAACAGGCGGAAGAAAGTGCCGATAATATTGCTTTACGTTGGCTTAAGAAAAATGAAAGAACATTGATATAACAt                            <  1:896912/251‑1 (MQ=255)                                                                                                                                                                                                          aCTTGGATCTGGATAAAGTAGCAACAGGGGAACATTGGTTCGGTCAGCAGGCATTAAATTTAAATTTAGTGGATGAGATTATGACCAGCGATGATTTGCTATTACAGGCGATGAAAGAGAAATCGGTTATCGGCGTAAAATATGCGGTGAAGAAATCGTTATTACAAAAAGTCGGTAAACAGGCGGAAGAAAGTGCCGATAATATTGCTTTACGTTGGCTTAAGAAAAATGAAAGAACATTGATATAACAt                            <  1:826673/251‑1 (MQ=255)                                                                                                                                                                                                           cTTGGATCTGGATAAAGTAGCAACAGGGGAACATTGGTTCGGTCAGCAGGCAttaaat                                                                                                                                                                                                                            >  1:765652/1‑58 (MQ=255)                                                                                                                                                                                                                    ggATAAAGTAGCAACAGGGGAACATTGGTTCGGTCAGCAGGCATTAAATTTAAATTTAGTGGATGAGATTATGAcc                                                                                                                                                                                                 >  1:45963/1‑76 (MQ=255)                                                                                                                                                                                                                    ggATAAAGTAGCAACAGGGGAACATTGGTTCGGTCAGCAGGCATTAAATTTAAATTTAGTGGATGAGATTATGACCAGCGATGATTTGCTATTACAGGCGATGAAAGAGAAATCGGTTATCGGCGTAAAATATGCGGTGAAGAAAt                                                                                                                           >  1:813792/1‑146 (MQ=255)                                                                                                                                                                                                                    ggATAAAGTAGCAACAGGGGAACATTGGTTCGGTCAGCAGGCATTAAATTTAAATTTAGTGGATGAGATTATGACCAGCGATGATTTGCTATTACAGGCGATGAAAGAGAAATCGGTTATCGGCGTAAAATATGCGGTGAAGAAATc                                                                                                                          >  1:492977/1‑147 (MQ=255)                                                                                                                                                                                                                      aTAAAGTAGCAACAGGGGAACATTGGTTCGGTCAGCAGGCATTAAATTTAAATTTAGTGGATGAGATTATGACCAGCGATGATTTGCTATTACAGGCGATGAAAGAGAAATCGGTTATCGGCGTAAAATATGCGGTGAAGAAATCGTTATTACAAAAAGTCGGTAAACAGGCGGAAGAAAGTGCCGATAATATTGCTTTACGTTGGCTTAAGAAAAATGAAAGAACATTGATATAACATCCTAttttttt                 <  1:276420/250‑1 (MQ=255)                                                                                                                                                                                                                          aGTAGCAACAGGGGAACATTGGTTCGGTCAGCAGGCATTAAATTTAAAATTAGTGGATGAGATTATGACCAGCGATGATTTGCTATTACAGGCGATGAAAGAGAAATCGGTTATCGGCGTAAAATATGCGGTGAAGAAATCGTTATTACAAAAAGTCGGTAAACAGGCGGAAGAAAGTGCCGATAATATTGCTTTACGCTGGCTTAAGAAAAATGAAAGAACATTGATATAACATCCTATTTTTTTCGAtt            >  1:787690/1‑251 (MQ=255)                                                                                                                                                                                                                           gTAGCAACAGGGGAACATTGGTTCGGTCAGCAGGCATTAAATTTAAATTTAGTGGATGAGATTATGACCAGCGATGATTTGCTATTACAGGCGATGAAAGAGAAATCGGTTATCGGCGTAAAATATGCGGTGAAGAAATCGtt                                                                                                                       >  1:84943/1‑143 (MQ=255)                                                                                                                                                                                                                                  cAGGGGAACATTGGTTCGGTCAGCAGGCATTAAATTTAAATTTAGTGGATGAGATTATGACCAGCGATGATTTGCTATTACAGGCGATGAAAGAGAAATCGGTTATCGGCGTAAAATATGCGGTGAAGAAATCGTTATTACAAAAAGTCGGTAAACAGGCGGAAGAAAGTGCCGATAATATTGCTTTACGt                                                                >  1:618985/1‑191 (MQ=255)                                                                                                                                                                                                                                  cAGGGGAACATTGGTTCGGTCAGCAGGCATTAAATTTAAATTTAGTGGATGAGATTATGACCAGCGATGATTTGCTATTACAGGCGATGAAAGAGAAATCGGTTATCGGCGTAAAATATGCGGTGAAGAAATCGTTATTACAAAAAGTCGGTAAACAGGCGGAAGAAAGTGCCGATAATATTGCTTTACGTTGGCTTAAGa                                                      >  1:610960/1‑201 (MQ=255)                                                                                                                                                                                                                                    ggggAACATTGGTTCGGTCAGCAGGCATTAAATTTAAATTTAGTGGATGAGATTATGACCAGCGATGATTTGCTATTACAGGCGATGAAAGAGAAATCGGTTATCGGCGTAAAATATGCGGTGAAGAAATCGTTATTACAAAAAGTCGGTAAACAGGCGGAAGAAAGTGCCGATAATATTg                                                                        >  1:615202/1‑181 (MQ=255)                                                                                                                                                                                                                                     gggAACATTGGTTCGGTCAGCAGGCATTAAATTTAAATTTAGTGGATGAGATTATGACCAGCGATGATTTGCTATTACAGGCGATGAAAGAGAAATCGGTTATCGGCGTAAAATATGCGGTGAAGAAATCGTTATTACAAAAAGTCGGTAAACAGGCGGAAGAAAGTGCCGATAATATTGCTTTACGTTGGCTTAAGAAAAATGAAAGAACATTGATATAACATCCTATTTTTTTCGATTCTATTTATAc  <  1:550210/250‑1 (MQ=255)                                                                                                                                                                                                                                      ggAACATTGGTTCGGTCAGCAGGCATTAAATTTAAATTTAGTGGATGAGATTATGACCAGCGATGATTTGCTATTACAGGCGATGAAAGAGAAATCGGTTATCGGCGTAAAATATGCGGTGAAGAAATCGTTATTACAAAAAGTCGGTAAACAGGCGGAAGAAAGTGCCGATAATATTGCTTTACGTTGGCTTAAGAAAAATGAAAGAACATTGATATAACa                             >  1:785516/1‑222 (MQ=255)                                                                                                                                                                                                                                       gAACATTGGTTCGGTCAGCAGGCATTAAATTTAAATTTAGTGGATGAGATTATGACCAGCGATGATTTGCTATTACAGGCGATGAAAGAGAAATCGGTTATCGGCGTAAAATATGCGGTGAAGAAATCGTTATTACAAAAAGTCGGTAAACAGGCGGAAGAAAGTGCCGATAATATTGCTTTACGTTGGCTTAAGAAAAATGAAAGAACATTGATATAAc                              >  1:212256/1‑220 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                             TCGGTGTGGTAGCGCAGATTCCAAATGTTCATCGCCTACTAAAAAAACATGATGTGGATGTGGACGTGATGACGGCAGGCGAATATAAACGCACTATGACGATTTTTGGTGAAAATACCGAAAAAGGAAAGCAAAAATTCCAACAGGAATTAGAAGAAACTCATCAGTTATTCAAGCAATTTGTCGCACAGAATCGACCGCACTTGGATCTGGATAAAGTAGCAACAGGGGAACATTGGTTAGGTCAGCAGGCATTAAATTTAAATTTAGTGGATGAGATTATGACCAGCGATGATTTGCTATTACAGGCGATGAAAGAGAAATCGGTTATCGGCGTAAAATATGCGGTGAAGAAATCGTTATTACAAAAAGTCGGTAAACAGGCGGAAGAAAGTGCCGATAATATTGCTTTACGTTGGCTTAAGAAAAATGAAAGAACATTGATATAACATCCTATTTTTTTCGATTCTATTTATAC  >  1/1226104‑1226581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑