Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,183,721	T→C	intergenic (+44591/+209590)	unknown → / ← unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,183,721	0	T	C	100.0%	196.4 / NA	68	intergenic (+44591/+209590)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/33);  total (35/33)

GAATCCAAATTAATACCGCACTTTCGTTTCGTGGTGGATTGGTTCTTCCAACAATTACAAGCCAATTCCGGTTTTTTAAATTCAACCCTGAACTTGGAGCAGATTGCCAATCCGGCAACAAATGCGGTTCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAATGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAACGGCGGCGCCAGTTGGCGTATTGCATACTGGTGCCCGGGATGTTGACCGGTTCGGTCATACCCAGCCAGTCTTCCGGTTGGAAGCCGAACAGGGCGCTGTTGACC  >  1/1183477‑1183959                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                               gAATCCAAATTAATACCGCACTTTCGTTTCGTGGTGGATTGGTTCTTCCAACAATTACAAGCCAATTCCGGTTTTTTAAATTCAACCCTGAACTTGGAGCAGATTGCCAATCCGGCAACAAATGCGGTTCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGttttt                                                                                                                                                                                                                                          >  1:837796/1‑251 (MQ=255)   aTCCAAATTAATACCGCACTTTCGTTTCGTGGTGGATTGGTTCTTCCAACAATTACAAGCCAATTCCGGTTTTTTAAATTCAACCCTGAACTTGGAGCAGATTGCCAATCCGGCAACAAATGCGGTTCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTaa                                                                                                                                                                                                                                        <  1:405282/251‑1 (MQ=255)        aaTTAATACCGCACTTTCGTTTCGTGGTGGATTGGTTCTTCCAACAATTACAAGCCAATTCCGGTTTTTTAAATTCAACCCTGAACTTGGAGCAGATTGCCAATCCGGCAACAAATGCGGTTCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAAcc                                                                                                                                                                                                                                    >  1:150532/1‑250 (MQ=255)        aaTTAATACCGCACTTTCGTTTCGTGGTGGATTGGTTCTTCCAACAATTACAAGCCAATTCCGGTTTTTTAAATTCAACCCTGAACTTGGAGCAGATTGCCAATCCGGCAACAAATGCGGTTCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAAcc                                                                                                                                                                                                                                    >  1:817220/1‑250 (MQ=255)                     cTTTCGTTTCGTGGTGGATTGGTTCTTCCAACAATTACAAGCCAATTCCGGTTTTTTAAATTCAACCCTGAACTTGGAGCAGATTGCCAATCCGGCAACAAATGCGGTTCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAAc                                                                                                                                                                                                                                     >  1:75045/1‑236 (MQ=255)                       ttcgtttcgtGGTGGATTGGTTCTTCCAACAATTACAAGCCAATTCCGGTTTTTTAAATTCAACCCTGAACTTGGAGCAGATTGCCAATCCGGCAACAAATGCGGTTCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCAtt                                                                                                                                                                                                                    <  1:58511/251‑1 (MQ=255)                       ttcgtttcgtGGTGGATTGGTTCTTCCAACAATTACAAGCCAATTCCGGTTTTTTAAATTCAACCCTGAACTTGGAGCAGATTGCCAATCCGGCAACAAATGCGGTTCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCAtt                                                                                                                                                                                                                    <  1:645776/251‑1 (MQ=255)                         cgtttcgtGGTGGATTGGTTCTTCCAACAATTACAAGCCAATTCCGGTTTTTTAAATTCAACCCTGAACTTGGAGCAGATTGCCAATCCGGCAACAAATGCGGTTCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTta                                                                                                                                                                                                                  <  1:375387/251‑1 (MQ=255)                          gtttcgtGGTGGATTGGTTCTTCCAACAATTACAAGCCAATTCCGGTTTTTTAAATTCAACCCTGAACTTGGAGCAGATTGCCAATCCGGCAACAAATGCGGTTCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTtat                                                                                                                                                                                                                 >  1:759422/1‑251 (MQ=255)                            ttcgtGGTGGATTGGTTCTTCCAACAATTACAAGCCAATTCCGGTTTTTTAAATTCAACCCTGAACTTGGAGCAGATTGCCAATCCGGCAACAAATGCGGTTCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTatat                                                                                                                                                                                                               <  1:111967/251‑1 (MQ=255)                            ttcgtGGTGGATTGGTTCTTCCAACAATTACAAGCCAATTCCGGTTTTTTAAATTCAACCCTGAACTTGGAGCAGATTGCCAATCCGGCAACAAATGCGGTTCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTatat                                                                                                                                                                                                               <  1:786859/251‑1 (MQ=255)                               gtggtgGATTGGTTCTTCCAACAATTACAAGCCAATTCCGGTTTTTTAAATTCAACCCTGAACTTGGAGCAGATTGCCAATCCGGCAACAAATGCGGTTCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTgg                                                                                                                                                                                                            >  1:600466/1‑251 (MQ=255)                                tggtggATTGGTTCTTCCAACAATTACAAGCCAATTCCGGTTTTTTAAATTCAACCCTGAACTTGGAGCAGATTGCCAATCCGGCAACAAATGCGGTTCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGt                                                                                                                                                                                                           >  1:803058/1‑251 (MQ=255)                                      aTTGGTTCTTCCAACAATTACAAGCCAATTCCGGTTTTTTAAATTCAACCCTGAACTTGGAGCAGATTGCCAATCCGGCAACAAATGCGGTTCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACAt                                                                                                                                                                                                      <  1:366704/250‑1 (MQ=255)                                        tGGTTCTTCCAACAATTACAAGCCAATTCCGGTTTTTTAAATTCAACCCTGAACTTGGAGCAGATTGCCAATCCGGCAACAAATGCGGTTCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCAGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCgg                                                                                                                                                                                                   >  1:436858/1‑251 (MQ=255)                                                ccAACAATTACAAGCCAATTCCGGTTTTTTAAATTCAACCCTGAACTTGGAGCAGATTGCCAATCCGGCAACAAATGCGGTTCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTAcc                                                                                                                                                                                           <  1:621841/251‑1 (MQ=255)                                                 caacaaTTACAAGCCAATTCCGGTTTTTTAAATTCAACCCTGAACTTGGAGCAGATTGCCAATCCGGCAACAAATGCGGTTCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTAccc                                                                                                                                                                                          <  1:241739/251‑1 (MQ=255)                                                 caacaaTTACAAGCCAATTCCGGTTTTTTAAATTCAACCCTGAACTTGGAGCAGATTGCCAATCCGGCAACAAATGCGGTTCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTAccc                                                                                                                                                                                          <  1:563345/251‑1 (MQ=255)                                                       ttACAAGCCAATTCCGGTTTTTTAAATTCAACCCTGAACTTGGAGCAGATTGCCAATCCGGCAACAAATGCGGTTCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACg                                                                                                                                                                                    <  1:896875/251‑1 (MQ=255)                                                        tACAAGCCAATTCCGGTTTTTTAAATTCAACCCTGAACTTGGAGCAGATTGCCAATCCGGCAACAAATGCGGTTCCGGTTTTAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGt                                                                                                                                                                                   <  1:434769/251‑1 (MQ=255)                                                            aGCCAATTCCGGTTTTTTAAATTCAACCCTGAACTTGGAGCAGATTGCCAATCCGGCAACAAATGCGGTTCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAg                                                                                                                                                                               >  1:468895/1‑251 (MQ=255)                                                                   tCCGGTTTTTTAAATTCAACCCTGAACTTGGAGCAGATTGCCAATCCGGCAACAAATGCGGTTCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCAc                                                                                                                                                                        <  1:95973/251‑1 (MQ=255)                                                                       gTTTTTTAAATTCAACCCTGAACTTGGAGCAGATTGCCAATCCGGCAACAAATGCGGTTCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCActtc                                                                                                                                                                     <  1:413650/250‑1 (MQ=255)                                                                              aaaTTCAACCCTGAACTTGGAGCAGATTGCCAATCCGGCAACAAATGCGGTTCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTAccc                                                                                                                                                                                          <  1:204475/222‑1 (MQ=255)                                                                               aaTTCAACCCTGAACTTGGAGCAGATTGCCAATCCGGCAACAAATGCGGTTCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCaa                                                                                                                                                            <  1:87792/251‑1 (MQ=255)                                                                                            aCTTGGAGCAGATTGCCAATCCGGCAACAAATGCGGTTCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGt                                                                                                                                                 >  1:307984/1‑249 (MQ=255)                                                                                            aCTTGGAGCAGATTGCCAATCCGGCAACAAATGCGGTTCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGAAGGTGTTTAAAGTGCGGAGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGTGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGaa                                                                                                                                                    >  1:430053/1‑246 (MQ=255)                                                                                                  aGCAGATTGCCAATCCGGCAACAAATGCGGTTCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAACCGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATc                                                                                                                                          <  1:361295/250‑1 (MQ=255)                                                                                                                          aTGCGGTTCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATc                                                                                                                                          >  1:352987/1‑226 (MQ=255)                                                                                                                             cGGTTCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGtt                                                                                                              >  1:59473/1‑251 (MQ=255)                                                                                                                               gTTCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTta                                                                                                                                                                                                                  >  1:754729/1‑149 (MQ=255)                                                                                                                               gTTCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTaa                                                                                                            >  1:682375/1‑251 (MQ=255)                                                                                                                                 tCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAAc                                                                                                          <  1:885429/251‑1 (MQ=255)                                                                                                                                    ggTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAAcgg                                                                                                        >  1:77619/1‑250 (MQ=255)                                                                                                                                     gTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAAcggcg                                                                                                      >  1:655398/1‑251 (MQ=255)                                                                                                                                                   cTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGtt                                                                                                                                                                                                          <  1:284970/137‑1 (MQ=255)                                                                                                                                                   cTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAAcggctgt                                                                                                    <  1:177404/239‑4 (MQ=255)                                                                                                                                                   cTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAACGGCGGCGCCAGTTGGCGt                                                                                        >  1:460212/1‑251 (MQ=255)                                                                                                                                                        cTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAACGGCGGCGCCAGTTGGCGTATTGc                                                                                   <  1:424098/251‑1 (MQ=255)                                                                                                                                                            ttggttggGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAACGGCGGCGCCAGTTGGCGTATTGCATAc                                                                               <  1:494216/251‑1 (MQ=255)                                                                                                                                                              ggttggGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAACGGCGGCGCCAGTTGGCGTATTGCATa                                                                                >  1:181374/1‑248 (MQ=255)                                                                                                                                                                  gggATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAACGGCGGCGCCAGTTGGCGTATTGCATACTGGTg                                                                          >  1:560941/1‑250 (MQ=255)                                                                                                                                                                    gATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAAt                                                                                                                                                   >  1:220652/1‑175 (MQ=255)                                                                                                                                                                    gATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAACGGCGGCGCCAGTTGGCGTATTGCATACTGGTGcc                                                                        >  1:405165/1‑250 (MQ=255)                                                                                                                                                                         gATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAACGGCGGCGCCAGTTGGCg                                                                                         >  1:46138/1‑228 (MQ=255)                                                                                                                                                                         gATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAACGGCGGCGCCAGTTGGCGTATTGCATACTGGTGCCCGGGAt                                                                  >  1:690501/1‑251 (MQ=255)                                                                                                                                                                            gAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCActtc                                                                                                                                                                     >  1:72254/1‑149 (MQ=255)                                                                                                                                                                            gAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAACGGCGGCGCCAGTTGGCGTATTGCATAc                                                                               >  1:100533/1‑235 (MQ=255)                                                                                                                                                                                aCCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAACGGCGGCGCCAGTTGGCGTATTGCATACTGGTGCCCGGGATGTTGAcc                                                           <  1:495371/251‑1 (MQ=255)                                                                                                                                                                                 ccccTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAACGGCGGCGCCAGTTGGCGTATTGCATACTGGTGCCCGGGATGTTGACCg                                                          >  1:20574/1‑251 (MQ=255)                                                                                                                                                                                   ccTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAACGGCGGCGCCAGTTGGCGTATTGCATACTgg                                                                            >  1:536671/1‑231 (MQ=255)                                                                                                                                                                                          aCGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTCTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAACGGCGGCGCCAGTTGGCGTATTGCATACTGGTGCCCGGGATGTTGACCGGTTCGGTCa                                                 <  1:454251/251‑1 (MQ=255)                                                                                                                                                                                               atttatttGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTTTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAACGGCGGCGCCAGTTGGCGTATTGCATACTGGTGCCCGGAATGTTGACCGGTTCGGTCATAccc                                            <  1:398778/251‑1 (MQ=255)                                                                                                                                                                                               atttatttGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAACGGCGGCGCCAGTTGGCGTATTGCATACTGGTGCCCGGGATGTTGACCGGTTCGGTCATAccc                                            <  1:40178/251‑1 (MQ=255)                                                                                                                                                                                                  tatttGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAACGGCGGCGCCAGTTGGCGTATTGCATACTGGTGCCCGGGATGTTGACCGGTTCGGTCATACccagc                                         <  1:410974/251‑1 (MQ=255)                                                                                                                                                                                                   atttGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAACGGCGGCGCCAGTTGGCGTATTGCATACTGGTGCCCGGGATGTTGACCGGTTCGGTCATACccagc                                         >  1:326988/1‑250 (MQ=255)                                                                                                                                                                                                          cGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTCACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAACGGCGGCGCCAGTTGGCGTATTGCATACTGGTGCCCGGGATGTTGACCGGTTCGGTCATACCCAGCCAGTCTTc                                 <  1:436111/251‑1 (MQ=255)                                                                                                                                                                                                            ttGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAACGGCGGCGCCAGTTGGCGTATTGCATACTGGTGCCCGGGATGTTGACCGGTTCGGTCATACCCAGCCAGTCTTCCg                               <  1:42223/251‑1 (MQ=255)                                                                                                                                                                                                              ggCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAACGGCGGCGCCAGTTGGCGTATTGCATACTGGTGCCCGGGATGTTGACCGGTTCGGTCATACCCAGCCAGTCTTCCGGt                             >  1:753716/1‑251 (MQ=255)                                                                                                                                                                                                                   gggTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAACGGCGGCGCCAGTTGGCGTATTGCATACTGGTGCCCGGGATGTTGACCGGTTCGGTCATAccc                                            >  1:620190/1‑231 (MQ=255)                                                                                                                                                                                                                   gggTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAACGGCGGCGCCAGTTGGCGTATTGCATACTGGTGCCCGGGATGTTGACCGGTTCGGTCATACCCAGCCAGTCTTCCGGTTGGaa                        >  1:421535/1‑251 (MQ=255)                                                                                                                                                                                                                         gtgtTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAACGGCGGCGCCAGtt                                                                                             <  1:140848/176‑1 (MQ=255)                                                                                                                                                                                                                         gtgtTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAACGGCGGCGCCAGTTGGCGTATTGCATACTgg                                                                            >  1:177583/1‑193 (MQ=255)                                                                                                                                                                                                                         gtgtTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAACGGCGGCGCCAGTTGGCGTATTGCATACTgg                                                                            <  1:132863/193‑1 (MQ=255)                                                                                                                                                                                                                         gtgtTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAACGGCGGCGCCAGTTGGCGTATTGCATACTGGTGCCCGGGATGTTGACCGGTTCGGTCATAccc                                            <  1:710469/225‑1 (MQ=255)                                                                                                                                                                                                                          tgtTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAACGGCGGCGCCAGTTGGCGTATTGCATACTGGTGCCCGGGATGTTGACCGGTTCGGTCATACCCAGCCAGTCTTCCGGTTGGAAGCCGaa                  >  1:564335/1‑250 (MQ=255)                                                                                                                                                                                                                           gtTTAAAGTGCGGTGGGTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAACGGCGGCGCCAGTTGGCGTATTGCATACTGGTGCCCGGGATGTTGACCGGTTCGGTCATACCCAGCCAGTCTTCCGGTTGGAAGCCGAACa                >  1:859132/1‑251 (MQ=255)                                                                                                                                                                                                                                          ggTTTTAAAAACGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAACGGCGGCGCCAGTTGGCGTATTGCATACTGGTGCCCGGGATGTTGACCGGTTCGGTCATACCCAGCCAGTCTTCCGGTTGGAAGCCGAACAGGGCGCTGTTGAcc  <  1:205417/250‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                               GAATCCAAATTAATACCGCACTTTCGTTTCGTGGTGGATTGGTTCTTCCAACAATTACAAGCCAATTCCGGTTTTTTAAATTCAACCCTGAACTTGGAGCAGATTGCCAATCCGGCAACAAATGCGGTTCCGGTTTCAACTACAAACTAGTCTATTTGGTTGGGATCAGATGAATACCCCTTGGAACGGGATTTATTTGAACGTTGGCGGGGGTAGGTGTTTAAAGTGCGGTGGGTTTTAAAAATGTTTTTAAAACCCACCGCACTTTTCATTTATATTGGTTACATCGGGGCTTACCCTTTACGTTTAGCGTTCACTTCTTTCAGCAATTTCTGAATGTCTTGATCGGCAAAAATTGCGTCGGTGTTTTGCGTTAAACGGCGGCGCCAGTTGGCGTATTGCATACTGGTGCCCGGGATGTTGACCGGTTCGGTCATACCCAGCCAGTCTTCCGGTTGGAAGCCGAACAGGGCGCTGTTGACC  >  1/1183477‑1183959

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑