Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,183,057	T→C	intergenic (+43927/+210254)	unknown → / ← unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,183,057	0	T	C	100.0%	231.0 / NA	78	intergenic (+43927/+210254)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (48/30);  total (48/30)

CTTTGATTTAGCGGTGTTGTTAAGACAGGCGCAACAAAAATTAGGACGGGATTTCTGGCAGGATCTTTAAGCCTCGAATTTTCCATATTTATAAATATAACAATGGGATAGTAACGTCCTTTGGATCATTTGATCAAAAGGGCGCTTTTTTGAGCGCATAATTTTTTTGCGCAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATTTTTTTTCAACGCACAGAGATGATTTAATGATTGATTACATTATTATCGGCATTATTGTTTTTTCCATTATTGTCAGTTTATTACGCGGTTTTGTCCGCGAAGTCATGTCACTAGCCAGCTGGATCGTGGCATTTTTAATTGCAAGCAATTTTTATATGTATCTTGCCGGACTCTTAACATCAATTGAGTCCGTTTATATTCGTAACGGCACGGCTATCGGTATTTTAT  >  1/1182836‑1183285                                                                                                                                                                                                                              |                                                                                                                                                                                                                                     cttTGATTTAGCGGTGTTGTTAAGACAGGCGCAACAAAAATTAGGACGGGATTTCTGGCAGGATCTTTAAGCCTCGAATTTTCCATATTTATAAATATAACAATGGGATAGTAACGTCCTTTGGATCATTTGATCAAAAGGGCGCTTTTTTGAGCGCATAATTTTTTTGCGCAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAAtg                                                                                                                                                                                                         <  1:665980/251‑1 (MQ=255) cttTGATTTAGCGGTGTTGTTAAGACAGGCGCAACAAAAATTAGGACGGGATTTCTGGCAGGATCTTTAAGCCTCGAATTTTCCATATTTATAAATATAACAATGGGATAGTAACGTCCTTTGGATCATTTGATCAAAAGGGCGCTTTTTTGAGCGCATAATTTTTTTGCGCAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAAtg                                                                                                                                                                                                         >  1:571244/1‑251 (MQ=255)  tttGATTTAGCGGTGTTGTTAAGACAGGCGCAACAAAAATTAGGACGGGATTTCTGGCAGGATCTTTAAGCCTCGAATTTTCCATATTTATAAATATAACAATGGGATAGTAACGTCCTTTGGATCATTTGATCAAAAGGGCGCTTTTTTGAGCGCATAATTTTTTTGCGCAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAAtga                                                                                                                                                                                                        <  1:243859/251‑1 (MQ=255)      aTTTAGCGGTGTTGTTAAGACAGGCGCAACAAAAATTAGGACGGGATTTCTGGCAGGATCTTTAAGCCTCGAATTTTCCATATTTATAAATATAACAATGGGATAGTAACGTCCTTTGGATCATTTGATCAAAAGGGCGCTTTTTTGAGCGCATAATTTTTTTGCGCAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAAtgattga                                                                                                                                                                                                    >  1:333614/1‑251 (MQ=255)                  tgttAAGACAGGCGCAACAAAAATTAGGACGGGATTTCTGGCAGGATCTTTAAGCCTCGAATTTTCCATATTTATAAATATAACAATGGGATAGTAACGTCCTTTGGATCATTTGATCAAAAGTGCGCTTTTTTGAGCGCATAATTTTTTTGCGCAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAATGATTGATTACATtattat                                                                                                                                                                                        <  1:124163/251‑1 (MQ=255)                  tgttAAGACAGGCGCAACAAAAATTAGGACGGGATTTCTGGCAGGATCTTTAAGCCTCGAATTTTCCATATTTATAAATATAACAATGGGATAGTAACGTCCTTTGGATCATTTGATCAAAAGGGCGCTTTTTTGAGCGCATAATTTTTTTGCGCAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAgaga                                                                                                                                                                                                                   <  1:481398/224‑1 (MQ=255)                  tgttAAGACAGGCGCAACAAAAATTAGGACGGGATTTCTGGCAGGATCTTTAAGCCTCGAATTTTCCATATTTATAAATATAACAATGGGATAGTAACGTCCTTTGGATCATTTGATCAAAAGGGCGCTTTTTTGAGCGCATAATTTTTTTGCGCAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAgaga                                                                                                                                                                                                                   >  1:412993/1‑224 (MQ=255)                  tgttAAGACAGGCGCAACAAAAATTAGGACGGGATTTCTGGCAGGATCTTTAAGCCTCGAATTTTCCATATTTATAAATATAACAATGGGATAGTAACGTCCTTTGGATCATTTGATCAAAAGGGCGCTTTTTTGAGCGCATAATTTTTTTGCGCAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAAtgat                                                                                                                                                                                                       >  1:361856/1‑236 (MQ=255)                                    aaaaaTTAGGACGGGATTTCTGGCAGGATCTTTAAGCCTCGAATTTTCCATATTTATAAATATAACAATGGGATAGTAACGTCCTTTGGATCATTTGATCAAAAGGGCGCTTTTTTGAGCGCATAATTTTTTTGCGCAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAATGATTGATTACATTATTATCGGCATTATTGTTTTTTc                                                                                                                                                                      <  1:252524/251‑1 (MQ=255)                                                  gATTTCTGGCAGGATCTTTAAGCCTCGAATTTTCCATATTTATAAATATAACAATGGGATAGTAACGTCCTTTGGATCATTTGATCAAAAGGGCGCTTTTTTGAGCGCATAATTTTTTTGCGCAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAATGATTGATTACATTATTATCGGCATTATTGTTTTTTCCATTATTGTCAGt                                                                                                                                                         <  1:308123/250‑1 (MQ=255)                                                   aTTTCTGGCAGGATCTTTAAGCCTCGAATTTTCCATATTTATAAATATAACAATGGGATAGTAACGTCCTTTGGATCATTTGATCAAAAGGGCGCTTTTTTGAGCGCATAATTTTTTTGCGCAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAATGATTGATTACATTATTATCGGCATTATTGTTTTTTCCATTATTGTCAGttt                                                                                                                                                       <  1:462827/251‑1 (MQ=255)                                                               aTCTTTAAGCCTCGAATTTTCCATATTTATAAATATAACAATGGGATAGTAACGTCCTTTGGATCATTTGATCAAAAGGGCGCTTTTTTGAGCGCATAATTTTTTTGCGCAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAATGATTGATTACATTATTATCGGCATTATTGTTTTTTCCATTATTGTCAGTTTATTACGCGGttt                                                                                                                                           >  1:504854/1‑251 (MQ=255)                                                                tCTTTAAGCCTCGAATTTTCCATATTTATAAATATAACAATGGGATAGTAACGTCCTTTGGATCATTTGATCAAAAGGGCGCTTTTTTGAGCGCATAATTTTTTTGCGCAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAATGATTGATTACATTATTATCGGCATTATTGTTTTTTCCATTATTGTCAGTTTATTACGCGGtttt                                                                                                                                          >  1:32504/1‑251 (MQ=255)                                                                tCTTTAAGCCTCGAATTTTCCATATTTATAAATATAACAATGGGATAGTAACGTCCTTTGGATCATTTGATCAAAAGGGCGCTTTTTTGAGCGCATAATTTTTTTGCGCAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAATGATTGATTACATTATTATCGGCATTATTGTTTTTTCCATTATTGTCAGTTTATTACGCGGtttt                                                                                                                                          >  1:761581/1‑251 (MQ=255)                                                                tCTTTAAGCCTCGAATTTTCCATATTTATAAATATAACAATGGGATAGTAACGTCCTTTGGATCATTTGATCAAAAGGGCGCTTTTTTGAGCGCATAATTTTTTTGCGCAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAATGATTGATTACATTATTATCAGCATTATTGTTTTTTCCATTATTGTCAGTTTATTACGCGGtttt                                                                                                                                          >  1:852994/1‑251 (MQ=255)                                                                 cTTTAAGCCTCGAATTTTCCATATTTATAAATATAACAATGGGATAGTAACGTCCTTTGGATCATTTGATCAAAAGGGCGCTTTTTTGAGCGCATAATTTTTTTGCGCAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGcac                                                                                                                                                                                                                        >  1:826701/1‑172 (MQ=255)                                                                       gCCTCGAATTTTCCATATTTATAAATATAACAATGGGATAGTAACGTCCTTTGGATCATTTGATCAAAAGGGCGCTTTTTTGAGCGCATAATTTTTTTGCGCAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAATGATTGATTACATTATTATCGGCATTATTGTTTTTTCCATTATTGTCAGTTTATTACGCGGTTTTGTCcgcg                                                                                                                                   >  1:325375/1‑251 (MQ=255)                                                                                       atTTATAAATATAACAATGGGATAGTAACGTCCTTTGGATCATTTGATCAAAAGGGCGCTTTTTTGAGCGCATAATTTTTTTGCGCAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAATGATTGATTACATTATTATCGGCATTATTGTTTTTTcc                                                                                                                                                                     >  1:855976/1‑201 (MQ=255)                                                                                        tttATAAATATAACAATGGGATAGTAACGTCCTTTGGATCATTTGATCAAAAGGGCGCTTTTTTGAGCGCATAATTTTTTTGCGCAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAgaga                                                                                                                                                                                                                   >  1:240141/1‑154 (MQ=255)                                                                                        tttATAAATATAACAATGGGATAGTAACGTCCTTTGGATCATTTGATCAAAAGGGCGCTTTTTTGAGCGCATAATTTTTTTGCGCAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAATGATTGATTACATTATTATCGGCATTATTGTTTTTTCCATTATTGTCAGTTTATTACGCGGTTTTGTCCGCGAAGTCATGTCACTAGcc                                                                                                                  <  1:121414/251‑1 (MQ=255)                                                                                          tataAATATAACAATGGGATAGTAACGTCCTTTGGATCATTTGATCAAAAGGGCGCTTTTTTGAGCGCATAATTTTTTTGCGCAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAATGATTGATTACATTATTATCGGCATTATTGTTTTTTCCATTATTGTCAGTTTATTACGCGGTTTTGTCCGCGAAGTCATGTCACTAGCCAg                                                                                                                >  1:192378/1‑251 (MQ=255)                                                                                             aaaTATAACAATGGGATAGTAACGTCCTTTGGATCATTTGATCAAAAGGGCGCTTTTTTGAGCGCATAATTTTTTTGCGCAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAATGATTGATTACATTATTATCGGCATTATTg                                                                                                                                                                             >  1:17263/1‑187 (MQ=255)                                                                                             aaaTATAACAATGGGATAGTAACGTCCTTTGGATCATTTGATCAAAAGGGCGCTTTTTTGAGCGCATAATTTTTTTGCGCAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAATGATTGATTACATTATTATCGGCATTATTGTTTTTTCCATTATTGTCAGTTTATTACGCGGTTTTGTcc                                                                                                                                      >  1:630140/1‑226 (MQ=255)                                                                                             aaaTATAACAATGGGATAGTAACGTCCTTTGGATCATTTGATCAAAAGGGCGCTTTTTTGAGCGCATAATTTTTTTGCGCAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAATGATTGATTACATTATTATCGGCATTATTGTTTTTTCCATTATTGTCAGTTTATTACGCGGTTTTGTCCGCGAAGTCATGTCACTAGcc                                                                                                                  <  1:569232/246‑1 (MQ=255)                                                                                               atatAACAATGGGATAGTAACGTCCTTTGGATCATTTGATCAAAAGGGCGCTTTTTTGAGCGCATAATTTTTTTGCGCAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAAtga                                                                                                                                                                                                        >  1:688736/1‑158 (MQ=255)                                                                                               atatAACAATGGGATAGTAACGTCCTTTGGATCATTTGATCAAAAGGGCGCTTTTTTGAGCGCATAATTTTTTTGCGCAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAAtg                                                                                                                                                                                                         <  1:766052/157‑1 (MQ=255)                                                                                               atatAACAATGGGATAGTAACGTCCTTTGGATCATTTGATCAAAAGGGCGCTTTTTTGAGCGCATAATTTTTTTGCGCAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAATGATTGATTACATTATTATCGGCATTATTGTTTTTTCCATTATTGTCAGTTTATTACGCGGTTTTGTCCGCGAAGTCATGTCACTAGCCAGCTGGa                                                                                                           >  1:416260/1‑251 (MQ=255)                                                                                               atatAACAATGGGATAGTAACGTCCTTTGGATCATTTGATCAAAAGGGCGCTTTTTTGAGCGCATAATTTTTTTGCGCAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAATGATTGATTACATTATTATCGGCATTATTGTTTTTTCCATTATTGTCAGTTTATTACGCGGTTTTGTCCGCGAAGTCATGTCACTAGCCAGCTGGa                                                                                                           >  1:421835/1‑251 (MQ=255)                                                                                               atatAACAATGGGATAGTAACGTCCTTTGGATCATTTGATCAAAAGGGCGCTTTTTTGAGCGCATAATTTTTTTGCGCAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAATGATTGATTACATTATTATCGGCATTATTGTTTTTTCCATTATTGTCAGTTTATTACGCGGTTTTGTCCGCGAAGTCATGTCACTAGCCAGCTGGa                                                                                                           >  1:15990/1‑251 (MQ=255)                                                                                                  taACAATGGGATAGTAACGTCCTTTGGATCATTTGATCAAAAGGGCGCTTTTTTGAGCGCATAATTTTTTTGCGCAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAATGATTGATTACATTATTATCGGCATTATTGttt                                                                                                                                                                          >  1:338011/1‑185 (MQ=255)                                                                                                     cAATGGGATAGTAACGTCCTTTGGATCATTTGATCAAAAGGGCGCTTTTTTGAGCGCATAATTTTTTTGCGCAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAATGATTGATTACATTATTATCGGCATTATTGTTTTTTCCATTATTGTc                                                                                                                                                            >  1:568675/1‑196 (MQ=255)                                                                                                     cAATGGGATAGTAACGTCCTTTGGATCATTTGATCAAAAGGGCGCTTTTTTGAGCGCATAATTTTTTTGCGCAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAATGATTGATTACATTATTATCGGCATTATTGTTTTTTCCATTATTGTCAGTTTATTACGCGGTTTTGTCCGCGAAGTCATGTCACTAGCCAGCTGGATCGTgg                                                                                                     >  1:563766/1‑251 (MQ=255)                                                                                                       aTGGGATAGTAACGTCCTTTGGATCATTTGATCAAAAGGGCGCTTTTTTGAGCGCATAATTTTTTTGCGCAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAATGATTGATTACat                                                                                                                                                                                              >  1:850432/1‑160 (MQ=255)                                                                                                         gggATAGTAACGTCCTTTGGATCATTTGATCAAAAGGGCGCTTTTTTGAGCGCATAATTTTTTTGCGCAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGAtt                                                                                                                                                                                                              <  1:171275/142‑1 (MQ=255)                                                                                                         gggATAGTAACGTCCTTTGGATCATTTGATCAAAAGGGCGCTTTTTTGAGCGCATAATTTTTTTGCGCAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAATGATTGATTACATTATTATCgg                                                                                                                                                                                     >  1:562546/1‑167 (MQ=255)                                                                                                         gggATAGTAACGTCCTTTGGATCATTTGATCAAAAGGGCGCTTTTTTGAGCGCATAATTTTTTTGCGCAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAATGATTGATTACATTATTATCGGCATTATTGTTTTTTCCATTATTGTCAGTTTATTACGCGGTTTTGTCCGCGAAGTCATGTCACTAGCCAGCTGGATCGTGGCAtt                                                                                                 >  1:653828/1‑251 (MQ=255)                                                                                                          ggATAGTAACGTCCTTTGGATCATTTGATCAAAAGGGCGCTTTTTTGAGCGCATAATTTTTTTGCGCAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGAtt                                                                                                                                                                                                              >  1:118961/1‑141 (MQ=255)                                                                                                          ggATAGTAACGTCCTTTGGATCATTTGATCAAAAGGGCGCTTTTTTGAGCGCATAATTTTTTTGCGCAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAATGATTGATTACatt                                                                                                                                                                                             <  1:781491/158‑1 (MQ=255)                                                                                                          ggATAGTAACGTCCTTTGGATCATTTGATCAAAAGGGCGCTTTTTTGAGCGCATAATTTTTTTGCGCAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAATGATTGATTACAttatta                                                                                                                                                                                         >  1:321852/1‑162 (MQ=255)                                                                                                          ggATAGTAACGTCCTTTGGATCATTTGATCAAAAGGGCGCTTTTTTGAGCGCATAATTTTTTTGCGCAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAATGATTGATTACATTATTATCGGCATTATTGTTTTTTCCATTATTg                                                                                                                                                              >  1:224084/1‑189 (MQ=255)                                                                                                          ggATAGTAACGTCCTTTGGATCATTTGATCAAAAGGGCGCTTTTTTGAGCGCATAATTTTTTTGCGCAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAATGATTGATTACATTATTATCGGCATTATTGTTTTTTCCATTATTGTc                                                                                                                                                            >  1:200333/1‑191 (MQ=255)                                                                                                          ggATAGTAACGTCCTTTGGATCATTTGATCAAAAGGGCGCTTTTTTGAGCGCATAATTTTTTTGCGCAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAATGATTGATTACATTATTATCGGCATTATTGTTTTTTCCATTATTGTCAGTTTATTACGCGGTTTTGTCCGCGAAGTCATGTc                                                                                                                         <  1:525211/226‑1 (MQ=255)                                                                                                          ggATAGTAACGTCCTTTGGATCATTTGATCAAAAGGGCGCTTTTTTGAGCGCATAATTTTTTTGCGCAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAATGATTGATTACATTATTATCGGCATTATTGTTTTTTCCATTATTGTCAGTTTATTACGCGGTTTTGTCCGCGAAGTCATGTCACTAGCCAGCTGGATCGTGGCAttt                                                                                                >  1:759612/1‑251 (MQ=255)                                                                                                          ggATAGTAACGTCCTTTGGATCATTTGATCAAAAGGGCGCTTTTTTGAGCGCATAATTTTTTTGCGCAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAATGATTGATTACATTATTATCGGCATTATTGTTTTTTCCATTATTGTCAGTTTATTACGCGGTTTTGTCCGCGAAGTCATGTCACTAGCCAGCTGGATCGTGGCAttt                                                                                                >  1:499117/1‑251 (MQ=255)                                                                                                          ggATAGTAACGTCCTTTGGATCATTTGATCAAAAGGGCGCTTTTTTGAGCGCATAATTTTTTTGCGCAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAATGATTGATTACATTATTATCGGCATTATTGTTTTTTCCATTATTGTCAGTTTATTACGCGGTTTTGTCCGCGAAGTCATGTCACTAGCCAGCTGGATCGTGGCAttt                                                                                                >  1:309169/1‑251 (MQ=255)                                                                                                          ggATAGTAACGTCCTTTGGATCATTTGATCAAAAGGGCGCTTTTTTGAGCGCATAATTTTTTTGCGCAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAATGATTGATTACATTATTATCGGCATTATTGTTTTTTCCATTATTGTCAGTTTATTACGCGGTTTTGTCCGCGAAGTCATGTCACTAGCCAGCTGGATCGTGGCAttt                                                                                                >  1:656238/1‑251 (MQ=255)                                                                                                          ggATAGTAACGTCCTTTGGATCATTTGATCAAAAGGGCGCTTTTTTGAGCGCATAATTTTTTTGCGCAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAATGATTGATTACATTATTATCGGCATTATTGTTTTTTCCATTATTGTCAGTTTATTACGCGGTTTTGTCCGCGAAGTCATGTCACTAGCCAGCTGGATCGTGGCAttt                                                                                                >  1:383136/1‑251 (MQ=255)                                                                                                          ggATAGTAACGTCCTTTGGATCATTTGATCAAAAGGGCGCTTTTTTGAGCGCATAATTTTTTTGCGCAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAATGATTGATTACATTATTATCGGCATTATTGTTTTTTCCATTATTGTCAGTTTATTACGCGGTTTTGTCCGCGAAGTCATGTCACTAGCCAGCTGGATCGTGGCAttt                                                                                                >  1:382017/1‑251 (MQ=255)                                                                                                               gTAACGTCCTTTGGATCATTTGATCAAAAGGGCGCTTTTTTGAGCGCATAATTTTTTTGCGCAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAATGATTGATTACattat                                                                                                                                                                                           >  1:328354/1‑155 (MQ=255)                                                                                                               gTAACGTCCTTTGGATCATTTGATCAAAAGGGCGCTTTTTTGAGCGCATAATTTTTTTGCGCAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAATGATTGATTACatt                                                                                                                                                                                             <  1:57619/153‑1 (MQ=255)                                                                                                               gTAACGTCCTTTGGATCATTTGATCAAAAGGGCGCTTTTTTGAGCGCATAATTTTTTTGCGCAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAATGATTGATTACAttatta                                                                                                                                                                                         >  1:246543/1‑157 (MQ=255)                                                                                                               gTAACGTCCTTTGGATCATTTGATCAAAAGGGCGCTTTTTTGAGCGCATAATTTTTTTGCGCAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAATGATTGATTACATTATTATCGGCATTATTGTTTTTTCCATTATTg                                                                                                                                                              <  1:567029/184‑1 (MQ=255)                                                                                                                      ccTTTGGATCATTTGATCAAAAGGGCGCTTTTTTGAGCGCATAATTTTTTTGCGCAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAgaga                                                                                                                                                                                                                   >  1:68817/1‑124 (MQ=255)                                                                                                                      ccTTTGGATCATTTGATCAAAAGGGCGCTTTTTTGAGCGCATAATTTTTTTGCGCAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAATGATTGATTACat                                                                                                                                                                                              <  1:141738/145‑1 (MQ=255)                                                                                                                      ccTTTGGATCATTTGATCAAAAGGGCGCTTTTTTGAGCGCATAATTTTTTTGCGCAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAATGATTGATTACATTATTATCGGCATTATTGTTTTTTCCATTATTGTCAGTTTATTACGCGGTTTTGTCCGCGAAGTCATGTCACTAGCCAGCTGGATCGTGGCATTTTTAATTgcaag                                                                                     >  1:646097/1‑250 (MQ=255)                                                                                                                           ggATCATTTGATCAAAAGGGCGCTTTTTTGAGCGCATGATTTTTTTGCGAAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGAtt                                                                                                                                                                                                              <  1:331879/124‑1 (MQ=255)                                                                                                                           ggATCATTTGATCAAAAGGGCGCTTTTTTGAGCGCATAATTTTTTTGCGCAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAATGATTGATTACATTATTATCGGCATTATTGTTTTTTCCATTATTGTCAGTTTATTACGCGGTTTTGTCCGCGAAGTCATGTCACTAGCCAGCTGGATCGTGGCATTTTTAATTGCAAGCAAttt                                                                               >  1:700136/1‑251 (MQ=255)                                                                                                                                  ttGATCAAAAGGGCGCTTTTTTGAGCGCATAATTTTTTTGCGCAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAATGATTGATTACATTATTATCGGCATTATTGTTTTTTCCATTATTGTCAGTTTATTACGCGGTTTTGTCCGCGAAGTCATGTCACTAGCCAGCTGGATCGTGGCATTTTTAATTGCAAGCAATTTTTATATg                                                                        <  1:439013/251‑1 (MQ=255)                                                                                                                                    gATCAAAAGGGCGCTTTTTTGAGCGCATAATTTTTTTGCGCAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGAtt                                                                                                                                                                                                              >  1:727910/1‑115 (MQ=255)                                                                                                                                    gATCAAAAGGGCGCTTTTTTGAGCGCATAATTTTTTTGCGCAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAATGATTGATTACATTATTATCGGCATTATTGTTTTTTCCATTATTGTCAGTTTATTACGCGGTTTTGTCCGCGAAGTCATGTCACTAGCCAGCTGGATCGTGGCATTTTTAATTGCAAGCAATTTTTATATGTa                                                                      >  1:487401/1‑251 (MQ=255)                                                                                                                                     aTCAAAAGGGCGCTTTTTTGAGCGCATAATTTTTTTGCGCAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAATGATTGATTACATTATTATCGGCATTATTGTTTTTTCCATTATTGTCAGTTTGTTACGCGGTTTTGTCCGCGAAGTCATGTCAc                                                                                                                       >  1:426950/1‑201 (MQ=255)                                                                                                                                          aaGGGCGCTTTTTTGAGCGCATAATTTTTTTGCGCAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAATGATTGATTACATTATTATCGGCATTATTGTTTTTTCCATTATTGTCAGTTTATTACGCGGTTTTGTCCGCGAAGTCATGTCACTAGCCAGCTGGATCGTGGCATTTTTAATTGCAAGCAATTTTTATATGTATCTTGc                                                                <  1:879208/251‑1 (MQ=255)                                                                                                                                                              aTAATTTTTTTGCGCAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAATGATTGATTACATTATTATCGGCATTATTGTTTTTTCCATTATTGTCAGTTTATTACGCGGTTTTGTCCGCGAAGTCATGTCACTAGCCAGCTGGATCGTGGCATTTTTAATTGCAAGCAATTTTTATATGTATCTTGCCGGACTCTTAACATCAATTg                                            <  1:793811/251‑1 (MQ=255)                                                                                                                                                               tAATTTTTTTGCGCAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAATGATTGATTACATTATTATCGGCATTATTGTTTTTTCCATTATTGTCAGTTTATTACGCGGTTTTGTCCGCGAAGTCATGTCACTAGCCAGCTGGATCGTGGCATTTTTAATTGCAAGCAATTTTTATATg                                                                        <  1:312804/222‑1 (MQ=255)                                                                                                                                                                            cAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAATGATTGATTACATTATTATCGGCATTATTGTTTTTTCCATTATTGTCAGTTTATTACGCGGTTTTGTCCGCGAAGTCATGTCACTAGCCAGCTGGATCGTGGCATTTTTAATTGCAAGCAATTTTTATATGTATCTTGCCGGACTCTTAACATCAATTGAGTCCGTTTatat                               <  1:721300/250‑1 (MQ=255)                                                                                                                                                                                 gTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAATGATTGATTACATTATTATCGGCATTATTGTTTTTTCCATTATTGTCAGTTTATTACGCGGTTTTGTCCGCGAAGTCATGTCACTAGCCAGCTGGATCGTGGCATTTTTAATTGCAAGCAATTTTTATATGTATCTTGCCGGACTCTTAACATCAATTGAGTCCGTTTATATTCGTaa                         >  1:884183/1‑251 (MQ=255)                                                                                                                                                                                          ttttGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAATGATTGATTACATTATTATCGGCATTATTGTTTTTTCCATTATTGTCAGTTTATTACGCGGTTTTGTCCGCGAAGTCATGTCACTAGCCAGCTGGATCGTGGCATTTTTAATTGCAAGCAATTTTTATATGTATCTTGCCGGACTCTTAACATCAATTGAGTCCGTTTATATTCGTAacggcacggc                <  1:140387/251‑1 (MQ=255)                                                                                                                                                                                             tGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAATGATTGATTACAttatta                                                                                                                                                                                         <  1:392249/79‑1 (MQ=255)                                                                                                                                                                                             tGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAATGATTGATTACATTATTATCGGc                                                                                                                                                                                    <  1:93680/84‑1 (MQ=255)                                                                                                                                                                                             tGCTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAATGATTGATTACATTATTATCGGCATTATTGTTTTTTCCATTATTGTCAGTTTATTACGCGGTTTTGTCCGCGAAGTCATGTCACTAGCCAGCTGGATCGTGGCATTTTTAATTGCAAGCAATTTTtata                                                                          >  1:634244/1‑190 (MQ=255)                                                                                                                                                                                               cTAGAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAATGATTGATTACATTATTATCGGCATTATTGTTTTTTCCATTATTGTc                                                                                                                                                            <  1:541030/106‑1 (MQ=255)                                                                                                                                                                                                  gAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAATGATTGATTACATTATTATCGGCATTATTGTTTTTTCCATTATTGTCAGTTTATTACGCGGTTTTGTCCGCGAAGTCATGTCACTAGCCAGCTGGATCGTGGCATTTTTAATTGCAAGCAATTTTTATATGTATCTTGCCGGACTCTTAACATCAATTGAGTCCGTTTATATTCGTAACGGCACGGCTATCgg          >  1:194518/1‑249 (MQ=255)                                                                                                                                                                                                  gAATAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAATGATTGATTACATTATTATCGGCATTATTGTTTTTTCCATTATTGTCAGTTTATTACGCGGTTTTGTCCGCGAAGTCATGTCACTAGCCAGCTGGATCGTGGCATTTTTAATTGCAAGCAATTTTTATATGTATCTTGCCGGACTCTTAACATCAATTGAGTCCGTTTATATTCGTAACGGCACGGCTATCGGta        >  1:40876/1‑251 (MQ=255)                                                                                                                                                                                                   aaTAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAATGATTGATTACATTATTATCGGCATTATTGTTTTTTCCATTATTGTCAGTTTATTACGCGGTTTTGTCCGCGAAGTCATGTCACTAGCCAGCTGGATCGTGGCATTTTTAATTGCAAGCAATTTTTATATGTATCTTGCCGGACTCTTAACATCAATTGAGTCCGTTTATATTCGTAACGGCACGGCTATCGGt         <  1:692525/249‑1 (MQ=255)                                                                                                                                                                                                   aaTAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAATGATTGATTACATTATTATCGGCATTATTGTTTTTTCCATTATTGTCAGTTTATTACGCGGTTTTGTCCGCGAAGTCATGTCACTAGCCAGCTGGATCGTGGCATTTTTAATTGCAAGCAATTTTTATATGTATCTTGCCGGACTCTTAACATCAATTGAGTCCGTTTATATTCGTAACGGCACGGCTATCGGt         <  1:528960/249‑1 (MQ=255)                                                                                                                                                                                                   aaTAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAATGATTGATTACATTATTATCGGCATTATTGTTTTTTCCATTATTGTCAGTTTATTACGCGGTTTTGTCCGCGAAGTCATGTCACTAGCCAGCTGGATCGTGGCATTTTTAATTGCAAGCAATTTTTATATGTATCTTGCCGGACTCTTAACATCAATTGAGTCCGTTTATATTCGTAACGGCACGGCTATCGGt         <  1:51813/249‑1 (MQ=255)                                                                                                                                                                                                    aTAGCGCCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAATGATTGATTACATTATTATCGGCATTATTGTTTTTTCCATTATTGTCAGTTTATTACGCGGTTTTGTCCGCGAAGTCATGTCGCTAGCCAGCTGGATCGTGGCATTTTTAATTGCAAGCAATTTTTATATGTATCTTGCCGGACTCTTAACATCAATTGAGTCCGTTTATATTCGTAACGGCACGGCTATCGGtatt      >  1:462301/1‑251 (MQ=255)                                                                                                                                                                                                        cgcCCATTGCAAATGAAAATATCTTTTTTCAACGCACAGAGATGATTTAATGATTGATTACATTATTATCGGCATTATTGTTTTTTCCATTATTGTCAGTTTATTACGCGGTTTTGTCCGCGAAGTCATGTCACTAGCCAGCTGGATCGTGGCATTTTTAATTGCAAGCAATTTTTATATGTATCTTGCCGGACTCTTAACATCAATTGAGTCCGTTTATATTCGTAACGGCACGGCTATCGGtattttat  <  1:129878/251‑1 (MQ=255)                                                                                                                                                                                                                              |                                                                                                                                                                                                                                     CTTTGATTTAGCGGTGTTGTTAAGACAGGCGCAACAAAAATTAGGACGGGATTTCTGGCAGGATCTTTAAGCCTCGAATTTTCCATATTTATAAATATAACAATGGGATAGTAACGTCCTTTGGATCATTTGATCAAAAGGGCGCTTTTTTGAGCGCATAATTTTTTTGCGCAAATGTTTTCGTTTTTTGCTAGAATAGCGCCCATTGCAAATGAAAATATTTTTTTTCAACGCACAGAGATGATTTAATGATTGATTACATTATTATCGGCATTATTGTTTTTTCCATTATTGTCAGTTTATTACGCGGTTTTGTCCGCGAAGTCATGTCACTAGCCAGCTGGATCGTGGCATTTTTAATTGCAAGCAATTTTTATATGTATCTTGCCGGACTCTTAACATCAATTGAGTCCGTTTATATTCGTAACGGCACGGCTATCGGTATTTTAT  >  1/1182836‑1183285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑