Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,172,641	G→A	intergenic (+33511/+220670)	unknown → / ← unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,172,641	0	G	A	100.0%	193.3 / NA	67	intergenic (+33511/+220670)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (36/31);  total (36/31)

GAACATTAAGTGTTTAAACAAACGGCGGCGCATGGTCATCACTACTTTGCCGGAGACCCATGCCAGACAGTAATTGGAAATAAAGCTGGAAATGCCGCGAAAGAGAATCAGCAGCACCACAAATACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACGATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCATAATAAATTTTCTGTCTTAGGTCAAAAAATAATGGCGCATTGTACCTTTATTTCGTGCTCAAGCCAATCAATCTGCGATACCAAGGCGAAAATTCAGCGCGCGCGGTCTGAATTTTGATCTCTTCATTGTAGAACAATAGACTGATTTG  >  1/1172395‑1172869                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                     gAACATTAAGTGTTTAAACAAACGGCGGCGCATGGTCATCACTACTTTGCCGGAGACCCATGCCAGACAGTAATTGGAAATAAAGCTGGAAATGCCGCGAAAGAGAATCAGCAGCACCACAAATACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCa                                                                                                                                                                                                                                  >  1:547300/1‑251 (MQ=255)  aaCATTAAGTGTTTAAACAAACGGCGGCGCATGGTCATCACTACTTTGCCGGAGACCCATGCCAGACAGTAATTGGAAATAAAGCTGGAAATGCCGCGAAAGAGAATCAGCAGCACCACAAATACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCaa                                                                                                                                                                                                                                 >  1:763756/1‑251 (MQ=255)           tgtTTAAACAAACGGCGGCGCATGGTCATCACTACTTTGCCGGAGACCCATGCCAGACAGTAATTGGAAATAAAGCTGGAAATGCCGCGAAAGAGAATCAGCAGCACCACAAATACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCtt                                                                                                                                                                                                                         <  1:186619/250‑1 (MQ=255)               tAAACAAACGGCGGCGCATGGTCATCACTACTTTGCCGGAGACCCATGCCAGACAGTAATTGGAAATAAAGCTGGAAATGCCGCGAAAGAGAATCAGCAGCACCACAAATACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATAc                                                                                                                                                                                                                    <  1:76100/251‑1 (MQ=255)               tAAACAAACGGCGGCGCATGGTCATCACTACTTTGCCGGAGACCCATGCCAGACAGTAATTGGAAATAAAGCTGGAAATGCCGCGAAAGAGAATCAGCAGCACCACAAATACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGATTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATAc                                                                                                                                                                                                                    <  1:62329/251‑1 (MQ=255)                 aacaaacGGCGGCGCATGGTCATCACTACTTTGCCGGAGACCCATGCCAGACAGTAATTGGAAATAAAGCTGGAAATGCCGCGAAAGAGAATCAGCAGCACCACAAATACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACgg                                                                                                                                                                                                                  >  1:337639/1‑251 (MQ=255)                  acaaacGGCGGCGCATGGTCATCACTACTTTGCCGGAGACCCATGCCAGACAGTAATTGGAAATAAAGCTGGAAATGCCGCGAAAGAGAATCAGCAGCACCACAAATACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACggg                                                                                                                                                                                                                 <  1:378109/251‑1 (MQ=255)                         gcggcgCATGGTCATCACTACTTTGCCGGAGACCCATGCCAGACAGTAATTGGAAATAAAGCTGGAAATGCCGCGAAAGAGAATCAGCAGCACCACAAATACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAatc                                                                                                                                                                                                           <  1:217689/250‑1 (MQ=255)                                   gTCATCACTACTTTGCCGGAGACCCATGCCAGACAGTAATTGGAAATAAAGCTGGAAATGCCGCGAAAGAGAATCAGCAGCACCACAAATACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATaa                                                                                                                                                                                                >  1:825115/1‑251 (MQ=255)                                    tcatcaCTACTTTGCCGGAGACCCATGCCAGACAGTAATTGGAAATAAAGCTGGAAATGCCGCGAAAGAGAATCAGCAGCACCACAAATACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATaaa                                                                                                                                                                                               <  1:129899/251‑1 (MQ=255)                                       tcaCTACTTTGCCGGAGACCCATGCCAGACAGTAATTGGAAATAAAGCTGGAAATGCCGCGAAAGAGAATCAGCAGCACCACAAATACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGt                                                                                                                                                                                            >  1:859661/1‑251 (MQ=255)                                          ctactTTGCCGGAGACCCATGCCAGACAGTAATTGGAAATAAAGCTGGAAATGCCGCGAAAGAGAATCAGCAGCACCACAAATACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTg                                                                                                                                                                                         >  1:808530/1‑251 (MQ=255)                                              tttGCCGGAGACCCATGCCAGACAGTAATTGGAAATAAAGCTGGAAATGCCGCGAAAGAGAATCAGCAGCACCACAAATACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAgg                                                                                                                                                                                     <  1:622686/251‑1 (MQ=255)                                                      agaCCCATGCCAGACAGTAATTGGAAATAAAGCTGGAAATGCCGCGAAAGAGAATCAGCAGCACCACAAATACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCAt                                                                                                                                                                              <  1:8136/250‑1 (MQ=255)                                                       gaCCCATGCCAGACAGTAATTGGAAATAAAGCTGGAAATGCCGCGAAAGAGAATCAGCAGCACCACAAATACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCAtgg                                                                                                                                                                            >  1:367238/1‑251 (MQ=255)                                                            aTGCCAGACAGTAATTGGAAATAAAGCTGGAAATGCCGCGAAAGAGAATCAGCAGCACCACAAATACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAatcat                                                                                                                                                                                                         >  1:513194/1‑217 (MQ=255)                                                                   aCAGTAATTGGAAATAAAGCTGGAAATGCCGCGAAAGAGAATCAGCAGCACCACAAATACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGt                                                                                                                                                                   <  1:547670/248‑1 (MQ=255)                                                                      gTAATTGGAAATAAAGCTGGAAATGCCGCGAAAGAGAATCAGCAGCACCACAAATACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAatca                                                                                                                                                                                                          >  1:58337/1‑206 (MQ=255)                                                                      gTAATTGGAAATAAAGCTGGAAATGCCGCGAAAGAGAATCAGCAGCACCACAAATACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAg                                                                                                                                                                     >  1:459517/1‑243 (MQ=255)                                                                       tAATTGGAAATAAAGCTGGAAATGCCGCGAAAGAGAATCAGCAGCACCACAAATACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGAt                                                                                                                                                             <  1:296735/250‑1 (MQ=255)                                                                       tAATTGGAAATAAAGCTGGAAATGCCGCGAAAGAGAATCAGCAGCACCACAAATACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGAt                                                                                                                                                             <  1:669384/250‑1 (MQ=255)                                                                       tAATTGGAAATAAAGCTGGAAATGCCGCGAAAGAGAATCAGCAGCACCACAAATACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATc                                                                                                                                                            <  1:187951/251‑1 (MQ=255)                                                                              aaaTAAAGCTGGAAATGCCGCGAAAGAGAATCAGCAGCACCACAAATACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGAt                                                                                                                                                             <  1:176744/243‑1 (MQ=255)                                                                              aaaTAAAGCTGGAAATGCCGCGAAAGAGAATCAGCAGCACCACAAATACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCat                                                                                                                                                      <  1:638148/250‑1 (MQ=255)                                                                                            aTGCCGCGAAAGAGAATCAGCAGCACCACAAATACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCATAATAAATTTTCTGTc                                                                                                                                       <  1:630896/251‑1 (MQ=255)                                                                                                       gagaATCAGCAGCACCACAAATACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAGTCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAgg                                                                                                                                                                    >  1:456370/1‑211 (MQ=255)                                                                                                         gaATCAGCAGCACCACAAATACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGAt                                                                                                                                                             >  1:651763/1‑216 (MQ=255)                                                                                                                  gcaCCACAAATACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCAt                                                                                                                                                                              >  1:224163/1‑190 (MQ=255)                                                                                                                     ccacAAATACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATAc                                                                                                                                                                                                                    >  1:694018/1‑149 (MQ=255)                                                                                                                     ccacAAATACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGAt                                                                                                                                                             <  1:66681/204‑1 (MQ=255)                                                                                                                     ccacAAATACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGATTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAAc                                                                                                                                                                                              >  1:874769/1‑171 (MQ=255)                                                                                                                            tACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATAc                                                                                                                                                                                                                    <  1:70653/142‑1 (MQ=255)                                                                                                                              ccGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCat                                                                                                                                                      >  1:260839/1‑202 (MQ=255)                                                                                                                              ccGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCATAATAAATTTTCTGTCTTAGGTCAAAAAATAATGGCGCATTGTACCTTTa                                                                                                     <  1:289811/251‑1 (MQ=255)                                                                                                                                gACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCATAATAAATTTTCTGTCTTAGGTCAAAAAATAATGGCGCATTGTACCTTTAtt                                                                                                   >  1:146937/1‑251 (MQ=255)                                                                                                                                 aCATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCATAATAAATTTTCTGTCTTAGGTCAAAAAATAATGGCGCATTGTACCTTTAttt                                                                                                  <  1:497769/251‑1 (MQ=255)                                                                                                                                  cATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCATAATAAATTTTCTGTCTTAGGTCaaaaaa                                                                                                                          <  1:766175/226‑1 (MQ=255)                                                                                                                                  cATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCATAATAAATTTTCTGTCTTAGGTCAAAAAATAATGGCGCATTGTACCTTTAttt                                                                                                  >  1:844379/1‑250 (MQ=255)                                                                                                                                       gTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCATAATAAATTTTCTGTCTTAGGTCAAAAAATAATGGCGCATTGTACCTTTa                                                                                                     >  1:771174/1‑242 (MQ=255)                                                                                                                                                           gCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCATAATAAATTTTCTGTCTTAGGTCAAAAAATAATGGCGCATTGTACCTTTAttt                                                                                                  >  1:8571/1‑225 (MQ=255)                                                                                                                                                               tGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCATAATAAATTTTCTGTCTTAGGTCAAAAAATAATGGCGCATTGTACCTTTa                                                                                                     >  1:873658/1‑218 (MQ=255)                                                                                                                                                               tGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCATAATAAATTTTCTGTCTTAGGTCAAAAAATAATGGCGCATTGTACCTTTATTTCGTGCTCAAGCCAATCAATCTGCGATACCa                                                                    >  1:405028/1‑251 (MQ=255)                                                                                                                                                               tGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCATAATAAATTTTCTGTCTTAGGTCAAAAAATAATGGCGCATTGTACCTTTATTTCGTGCTCAAGCCAATCAATCTGCGATACCa                                                                    <  1:545895/251‑1 (MQ=255)                                                                                                                                                                             tcCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAatca                                                                                                                                                                                                          <  1:862255/103‑1 (MQ=255)                                                                                                                                                                               cAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAAc                                                                                                                                                                                              >  1:412976/1‑113 (MQ=255)                                                                                                                                                                               cAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCATAATAAATTTTCTGTCTTAGGTCAAAAAATAATGGCGCATTGTACCTTTATTTCGTGCTCAAGCCAATCAATCTGCGATACCAAGGCGAAAATTCAgcg                                                    >  1:189035/1‑251 (MQ=255)                                                                                                                                                                               cAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCATAATAAATTTTCTGTCTTAGGTCAAAAAATAATGGCGCATTGTACCTTTATTTCGTGCTCAAGCCAATCAATCTGCGATACCAAGGCGAAAATTCAgcg                                                    >  1:432359/1‑251 (MQ=255)                                                                                                                                                                                              tAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCATAATAAATTTTCTGTCTTAGGTCAAAAAATAATGGCGCATTGTACCTTTATTTCGTGCTCAAGCCAATCAATCTGCGATACCAAGGCGAAAATTCAGCGCGCGCGGTCTGAAtt                                     <  1:96817/251‑1 (MQ=255)                                                                                                                                                                                               aaGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCATAATAAATTTTCTGTCTTAGGTCAAAAAATAATGGCGCATTGTACCTTTAttt                                                                                                  >  1:124659/1‑189 (MQ=255)                                                                                                                                                                                                          gCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCATAATAAATTTTCTGTCTTAGGTCAAAAAATAATGGCGCATTGTACCTTTATTTCGTGCTCAAGCCAATCAATCTGCGATACCAAGGCGAAAATTCAGCGCGCGCGGTCTGAATTTTGATCTCTTCa                         >  1:519313/1‑251 (MQ=255)                                                                                                                                                                                                           ccGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCATAATAAATTTTCTGTCTTAGGTCAAAAAATAATGGCGCATTGTACCTTTATTTCGTGCTCAAGCCAATCAATCTGCGATACCAAGGCGAAAATTCAGCGCGCGCGGTCTGAATTTTGATCTCTTc                          <  1:424338/249‑1 (MQ=255)                                                                                                                                                                                                              gAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCATAATAAATTTTCTGTCTTAGGTCAAAAAATAATGGCGCATTGTACCTTTATTTCGTGCTCAAGCCAATCAATCTGCGAt                                                                        <  1:536523/200‑1 (MQ=255)                                                                                                                                                                                                               aaTCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCATAATAAATTTTCTGTCTTAGGTCAAAAAATAATGGCGCATTGTACCTTTATTTCGTGCTCAAGCCAATCAATCTGCGATACCAAGGCGAAAATTCAGCGCGCGTGGTCTGAATTTTGATCTCTTCATTGt                     <  1:555009/250‑1 (MQ=255)                                                                                                                                                                                                                  cGGTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCATAATAAATTTTCTGTCTTAGGTCAAAAAATAATGGCGCATTGTACCTTTATTTCGTGCTCAAGCCAATCAATCTGCGATACCAAGGCGAAAATTCAGCGCGCGCGGTCTGAAttt                                    >  1:377782/1‑232 (MQ=255)                                                                                                                                                                                                                    gTTAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCATAATAAATTTTCTGTCTTAGGTCAAAAAATAATGGCGCATTGTACCTTTATTTCGTGCTCAAGCCAATCAATCTGCGATACCAAGGCGAAAATTCAGCGCGCGCGGTCTGAATTTTGATCTCTTCATTGTAGAAc                <  1:339100/250‑1 (MQ=255)                                                                                                                                                                                                                     ttAACGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCATAATAAATTTTCTGTCTTAGGTCAAAAAATAATGGCGCATTGTACCTTTATTTCGTGCTCAAGCCAATCAATCTGCGATACCAAGGCGAAAATTCAGCGCGCGCGGTCTGAATTTTGATCTCTTCATTGTAGAACaa              <  1:649337/251‑1 (MQ=255)                                                                                                                                                                                                                        aCGCGTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCATAATAAATTTTCTGTCTTAGGTCAAAAAATAATGGCGCATTGTACCTTTATTTCGTGCTCAAGCCAATCAATCTGCGATACCAAGGCGAAAATTCAGCGCGCGCGGTCTGAATTTTGATCTCTTCATTGTAGAACAATAg           <  1:157186/251‑1 (MQ=255)                                                                                                                                                                                                                         cgcgTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGGCTTGATCTTGCATAATAAATTTTCTGTCTTAGGTCAAAAAATAATGGCGCATTGTACCTTTATTTCGTGCTCAAGCCAATCAATCTGCGATACCAAGGCGAAAATTCAGCGCGCGCGGTCTGAATTTTGAt                                >  1:16209/1‑229 (MQ=255)                                                                                                                                                                                                                           cgTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCATAATAAATTTTCTg                                                                                                                                         >  1:793327/1‑122 (MQ=255)                                                                                                                                                                                                                           cgTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCATAATAAATTTTCTGTCTTAGGTCAAAAAATAATGGCGCATTGTACCTTTATTTCGTGCTCAAGCCAATCAATCTGCGATACCAAGGCGAAAATTCAGCGCGCGCGGTCTGAAtt                                     >  1:275325/1‑222 (MQ=255)                                                                                                                                                                                                                            gTTTAATACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAgg                                                                                                                                                                    >  1:380380/1‑94 (MQ=255)                                                                                                                                                                                                                                 aTACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCATAATAAATTTTCTGTCTTAGGt                                                                                                                                 >  1:766255/1‑124 (MQ=255)                                                                                                                                                                                                                                 aTACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCATAATAAATTTTCTGTCTTAGGTCAAAAAATAATGGCGCATTGTACCTTTATTTCGTGCTCAAGCCAATCAATCTGCGATACCAAGGCGAAAATTCAGCGCGCGCGGTCTGAATTTTGATCTCTTCATTGt                     >  1:242593/1‑232 (MQ=255)                                                                                                                                                                                                                                  tACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCATAATAAATTTTCTGTCTTAGGTCAAAAAATAATGGCGCATTGTACCTTTAt                                                                                                    <  1:749814/152‑1 (MQ=255)                                                                                                                                                                                                                                  tACTAACGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCATAATAAATTTTCTGTCTTAGGTCAAAAAATAATGGCGCATTGTACCTTTATTTCGTGCTCAAGCCAATCAATCTGCGATACCAAGGCGAAAATTCAGCGCGCGCGGTCTGAATTTTGATCTCTTCATTGTAGAACAATAGACTGATTTg  >  1:35965/1‑250 (MQ=255)                                                                                                                                                                                                                                       aCGCAACACCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCATAATAAATTTTCTGTCTTAGGTCAAAAAATAATGGCGCATTGTACCTTTATTTCGTGCTCAAGCCAATCAATCTGCGATACCAAGGCGAAAATTCAGCGCGCGCGGTCTGAATTTTGATCTCTTCATTGt                     >  1:688925/1‑226 (MQ=255)                                                                                                                                                                                                                                            acacCGGAAACAATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCATAATAAATTTTCTGTCTTAGGTCAAAAAATAATGGCGCATTGTACCTTTATTTCGTGCTCAAGCcaatcaa                                                                                <  1:439856/162‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                     GAACATTAAGTGTTTAAACAAACGGCGGCGCATGGTCATCACTACTTTGCCGGAGACCCATGCCAGACAGTAATTGGAAATAAAGCTGGAAATGCCGCGAAAGAGAATCAGCAGCACCACAAATACCGACATTTGTTTTAAAAAAGAAGCGTCAGCTTTGCCAAAACCATCATCCAATAAGGGTTTCAGTAAGAAAATTAAGCCGGAATCGGTTAACGCGTTTAATACTAACGCAACACCGGAAACGATCAAACCTGCTTTATACGGGCTAATCATCGGCCATAAACGTTTGAAGGTTTGCATGGTGGAAAGGTCTTGATCTTGCATAATAAATTTTCTGTCTTAGGTCAAAAAATAATGGCGCATTGTACCTTTATTTCGTGCTCAAGCCAATCAATCTGCGATACCAAGGCGAAAATTCAGCGCGCGCGGTCTGAATTTTGATCTCTTCATTGTAGAACAATAGACTGATTTG  >  1/1172395‑1172869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑