Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,159,864	C→T	intergenic (+20734/+233447)	unknown → / ← unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,159,864	0	C	T	100.0%	139.7 / NA	49	intergenic (+20734/+233447)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/24);  total (25/24)

CTGAAAAACGACAGTAATGGCAATCAACACATAAAAACATATCTATACTAAAAAATAAATTTGTGTAACAACTTTATGCGAAATAAAATCAGAGATTAATCAAATCACTAATAAAATTTTACCGCGCTAAGAGAACAAGTATGAGTAAACTAACAAAGGTCATTAATCAGACAATCCGGGTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCCAAATAACGTACAACTATCATGAGCTCTGTTATGTTTATAAAGCAGCCAAAAAATATGTGTTATTAAACGATATTAATCTAGAAGAGTACTTTAATGAGTACCCGCCCAAGGTTTAACAGAGAAAGGGATTTTATAAATAAATGCATAAAAACATAATTATGTTTTAGTATGATGGAGTAAATATGAATATCTTAGACGAACTAAAGAAAGTATTGGATTCAAAGCGTCCG  >  1/1159618‑1160094                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                       cTGAAAAACGACAGTAATGGCAATCAACACATAAAAACATATCTATACTAAAAAATAAATTTGTGTAACAACTTTATGCGAAATAAAATCAGAGATTAATCAAATCACTAATAAAATTTTACCGCGCTAAGAGAACAAGTATGAGTAAACTAACAAAGGTCATTAATCAGACAATCCGGGTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAAt                                                                                                                                                                                                                                    <  1:540199/251‑1 (MQ=255)   gAAAAACGACAGTAATGGCAATCAACACATAAAAACATATCTATACTAAAAAATAAATTTGTGTAACAACTTTATGCGAAATAAAATCAGAGATTAATCAAATCACTAATAAAATTTTACCGCGCTAAGAGAACAAGTATGAGTAAACTAACAAAGGTCATTAATCAGACAATCCGGGTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAAt                                                                                                                                                                                                                                    >  1:145166/1‑249 (MQ=255)                  tGGCAATCAACACATAAAAACATATCTATACTAAAAAATAAATTTGTGTAACAACTTTATGCGAAATAAAATCAGAGATTAATCAAATCACTAATAAAATTTTACCGCGCTAAGAGAACAAGTATGAGTAAACTAACAAAGGTCATTAATCAGACAAACCGGGTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATg                                                                                                                                                                                                                   <  1:817103/251‑1 (MQ=255)                   ggCAATCAACACATAAAAACATATCTATACTAAAAAATAAATTTGTGTAACAACTTTATGCGAAATAAAATCAGAGATTAATCAAATCACTAATAAAATTTTACCGCGCTAAGAGAACAAGTATGAGTAAACTAACAAAGGTCATTAATCAGACAATCCGGGTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGa                                                                                                                                                                                                                  >  1:648527/1‑251 (MQ=255)                           acacATAAAAACATATCTATACTAAAAAATAAATTTGTGTAACAACTTTATGCGAAATAAAATCAGAGATTAATCAAATCACTAATAAAATTTTACCGCGCTAAGAGAACAAGTATGAGTAAACTAACAAAGGTCATTAATCAGACAATCCGGGTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGtt                                                                                                                                                                                                          >  1:457684/1‑251 (MQ=255)                                      cATATCTATACTAAAAAATAAATTTGTGTAACAACTTTATGCGAAATAAAATCAGAGATTAATCAAATCACTAATAAAATTTTACCGCGCTAAGAGAGCAAGTATGAGTAAACTAACAAAGGTCATTAATCAGACAAGCCGGGTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTATGTTTATaaa                                                                                                                                                                                               <  1:632387/251‑1 (MQ=255)                                                  aaaaaaTAAATTTGTGTAACAACTTTATGCGAAATAAAATCAGAGATTAATCAAATCACTAATAAAATTTTACCGCGCTAAGAGAACAAGTATGAGTAAACTAACAAAGGTCATTAATCAGACAATCCGGGTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTATGTTTATAAAGCAGCCaaaaaa                                                                                                                                                                                   <  1:150938/251‑1 (MQ=255)                                                     aaataaatTTGTGTAACAACTTTATGCGAAATAAAATCAGAGATTAATCAAATCACTAATAAAATTTTACCGCGCTAAGAGAACAAGTATGAGTAAACTAACAAAGGTCATTAATCAGACAATCCGGGTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATaa                                                                                                                                                                                                                                  >  1:478148/1‑201 (MQ=255)                                                             ttGTGTAACAACTTTATGCGAAATAAAATCAGAGATTAATCAAATCACTAATAAAATTTTACCGCGCTAAGAGAACAAGTATGAGTAAACTAACAAAGGTCATTAATCAGACAATCCGGGTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTa                                                                                                                                                                                                         <  1:340027/218‑1 (MQ=255)                                                                  tAACAACTTTATGCGAAATAAAATCAGAGATTAATCAAATCACTAATAAAATTTTACCGCGCTAAGAGAACAAGTATGAGTAAACTAACAAAGGTCATTAATCAGACAATCCGGGTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTa                                                                                                                                                                                                         >  1:541272/1‑213 (MQ=255)                                                                  tAACAACTTTATGCGAAATAAAATCAGAGATTAATCAAATCACTAATAAAATTTTACCGCGCTAAGAGAACAAGTATGAGTAAACTAACAAAGGTCATTAATCAGACAATCCGGGTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTATGttt                                                                                                                                                                                                    >  1:66200/1‑218 (MQ=255)                                                                       acTTTATGCGAAATAAAATCAGAGATTAATCAAATCACTAATAAAATTTTACCGCGCCAAGAGAACAAGCATGAGTAAACTAACAAAGGTCATTAATCAGACAATCCGGGTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTATGTTTATAAAGTAGCCAAAAAATATGTGTTATTAAACGATAtt                                                                                                                                                              >  1:337322/1‑251 (MQ=255)                                                                        cTTTATGCGAAATAAAATCAGAGATTAATCAAATCACTAATAAAATTTTACCGCGCTAAGAGAACAAGTATGAGTAAACTAACAAAGGTCATTAATCAGACAATCCGGGTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTATGTTTATAAAGCAGCCAAAAAATATGTGTTATTAAACGATATTa                                                                                                                                                             >  1:464147/1‑251 (MQ=255)                                                                                 aaaTAAAATCAGAGATTAATCAAATCACTAATAAAATTTTACCGCGCTAAGAGAACAAGTATGAGTAAACTAACAAAGGTCATTAATCAGACAATCCGGGTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTATGTTTATAAAGCAGCCAAAAAATATGTGTTATTAAACGATATTAATc                                                                                                                                                          >  1:575945/1‑245 (MQ=255)                                                                                               aTTAATCAAATCACTAATAAAATTTTACCGCGCTAAGAGAACAAGTATGAGTAAACTAACAAAGGTCATTAATCAGACAATCCGGGTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTATGTTTATAAAGCAGCCAAAAAATATGTGTTATTAAACGATATTAATCTAGAAGAGTACTTTAATg                                                                                                                                        <  1:825494/249‑1 (MQ=255)                                                                                                  aatcaaatcaCTAATAAAATTTTACCGCGCTAAGAGAACAAGTATGAGTAAACTAACAAAGGTCATTAATCAGACAATCCGGGTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAAt                                                                                                                                                                                                                                    <  1:705916/154‑1 (MQ=255)                                                                                                  aatcaaatcaCTAATAAAATTTTACCGCGCTAAGAGAACAAGTATGAGTAAACTAACAAAGGTCATTAATCAGACAATCCGGGTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTAc                                                                                                                                                                                                                             >  1:620948/1‑161 (MQ=255)                                                                                                  aatcaaatcaCTAATAAAATTTTACCGCGCTAAGAGAACAAGTATGAGTAAACTAACAAAGGTCATTAATCAGACAATCCGGGTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTATGTTTATAAAGCAGCCAAAAAATATGTGTTATTAAACGATATTAATc                                                                                                                                                          <  1:355135/228‑1 (MQ=255)                                                                                                  aatcaaatcaCTAATAAAATTTTACCGCGCTAAGAGAACAAGTATGAGTAAACTAACAAAGGTCATTAATCAGACAATCCGGGTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTATGTTTATAAAGCAGCCAAAAAATATGTGTTATTAAACGATATTAATCTagaag                                                                                                                                                    <  1:179788/234‑1 (MQ=255)                                                                                                       aatcaCTAATAAAATTTTACCGCGCTAAGAGAACAAGTATGAGTAAACTAACAAAGGTCATTAATCAGACAATCCGGGTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTATGTTTATAAAGCAGCCAAAAAATATGTGTTATTAAACGATATTAATCTAGAAGAGTACTTTAATGAGTACCCGcc                                                                                                                              <  1:895794/251‑1 (MQ=255)                                                                                                          caCTAATAAAATTTTACCGCGCTAAGAGAACAAGTATGAGTAAACTAACAAAGGTCATTAATCAGACAATCCGGGTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTATGTTTATAAAGCAGCCAAAAAATATGTGTTATTAAACGATATTAATCTAGAAGAGTACTTTAATg                                                                                                                                        >  1:198912/1‑238 (MQ=255)                                                                                                            cTAATAAAATTTTACCGCGCTAAGAGAACAAGTATGAGTAAACTAACAAAGGTCATTAATCAGACAATCCGGGTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTATGTTTATAAAGCAGCCAAAAAATATGTGTTATTAAACGATATTAATCTAGAAGAGTACTTTAATGAGCACCCGCCCAAgg                                                                                                                         <  1:256814/251‑1 (MQ=255)                                                                                                                    aTTTTACCGCGCTAAGAGAACAAGTATGAGTAAACTAACAAAGGTCATTAATCAGACAATCCGGGTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTATGTTTATAAAGCAGCCAAAAAATATGTGTTATTAAACGATATTAATCTAGAAGAGTACTTTAATGAGTACCCGCCCAAGGTTTAACag                                                                                                                 <  1:159829/251‑1 (MQ=255)                                                                                                                               cTAAGAGAACAAGTATGAGTAAACTAACAAAGGTCATTAATCAGACAATCCGGGTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTATGTTTATAAAGCAGCCAAAAAATATGTGTTATTAAACGATATTAATCTAGAAGAGTACTTTAATGAGTACCCGCCCAAGGTTTAACAGAGAAAGGGAtt                                                                                                      <  1:717229/251‑1 (MQ=255)                                                                                                                                   gagaACAAGTATGAGTAAACTAACAAAGGTCATTAATCAGACAATCCGGGTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACt                                                                                                                                                                                                                         <  1:209322/132‑1 (MQ=255)                                                                                                                                     gaACAAGTATGAGTAAACTAACAAAGGTCATTAATCAGACAATCCGGGTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTATGTTTATAAAGCAGCCAAAAAATATGTGTTATTAAACGATATTAATCTAGAAGAGTACTTTAATGAGTACCCGCCCAAGGTTTAACag                                                                                                                 >  1:825201/1‑234 (MQ=255)                                                                                                                                          aGTATGAGTAAACTAACAAAGGTCATTAATCAGACAATCCGGGTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTATGTTTATAAAGCAGCCAAAAAATAtgtg                                                                                                                                                                             <  1:837118/169‑1 (MQ=255)                                                                                                                                          aGTATGAGTAAACTAACAAAGGTCATTAATCAGACAATCCGGGTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTATGTTTATAAAGCAGCCAAAAAATATGTGTTATTAAACGATATTAATCTAGAAGAGTACTTTAATGAGTACCCGCCCAAGGTTTAACAGAGAAAGGGATTTTAtaaataaa                                                                                           >  1:633356/1‑251 (MQ=255)                                                                                                                                           gTATGAGTAAACTAACAAAGGTCATTAATCAGACAATCCGGGTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTATGTTTATAAAGCAGCCAAAAAATAtgtg                                                                                                                                                                             >  1:547065/1‑168 (MQ=255)                                                                                                                                              tGAGTAAACTAACAAAGGTCATTAATCAGACAATCCGGGTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCCGTTa                                                                                                                                                                                                         <  1:782345/137‑1 (MQ=255)                                                                                                                                               gAGTAAACTAACAAAGGTCATTAATCAGACAATCCGGGTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTATGt                                                                                                                                                                                                      >  1:779548/1‑139 (MQ=255)                                                                                                                                                   aaaCTAACAAAGGTCATTAATCAGACAATCCGGGTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTATGTTTATAAAGCAGCCAAAAAATATGTGTTATTAAACGATATTAATCTagaag                                                                                                                                                    >  1:357609/1‑185 (MQ=255)                                                                                                                                                    aaCTAACAAAGGTCATTAATCAGACAATCCGGGTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTATGTTTATAAAGCAGCCAAAAAATATGTGTTATTAAACGATATTAATc                                                                                                                                                          <  1:571062/178‑1 (MQ=255)                                                                                                                                                    aaCTAACAAAGGTCATTAATCAGACAATCCGGGTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTATGTTTATAAAGCAGCCAAAAAATATGTGTTATTAAACGATATTAATCTAGAAGAGTACTTTaa                                                                                                                                          <  1:506008/194‑1 (MQ=255)                                                                                                                                                        aaCAAAGGTCATTAATCAGACAATCCGGGTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTATGttt                                                                                                                                                                                                    >  1:804979/1‑132 (MQ=255)                                                                                                                                                                  aTTAATCAGACAATCCGGGTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTATg                                                                                                                                                                                                       >  1:162341/1‑119 (MQ=255)                                                                                                                                                                          gACAATCCGGGTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTATGTTTATAAAGCAGCCAAAAAATATGTGTTATTAAACGATATTAATCTAGAAGAGTACttt                                                                                                                                            >  1:135511/1‑170 (MQ=255)                                                                                                                                                                            cAATCCGGGTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTATGTTTATAAAGCAGCCAAAAAATATGTGTTATTAAACGATATTAATCTAGAAGAGTACTTTAATGAGTACCCGCCCAAGGTTTAACAGAGAAAGGGATTTTATAAATAAATGCATAAAAACATAATTATGTTTTAGTATGATgg                                                         <  1:336309/251‑1 (MQ=255)                                                                                                                                                                                  gggTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTATGTTTATAAAGCAGCCAAAAAATATGTGTTATTAAACGATATTAATCTAGAAGAGTACTTTAATGAGTACCCGCCCAAGGTTTAACAGAGAAAGGGATTTTATAAATAAATGCATAAAAACATAATTATGTTTTAGTATGATGGAGTaaa                                                   >  1:514392/1‑251 (MQ=255)                                                                                                                                                                                       atttatGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACt                                                                                                                                                                                                                         >  1:330479/1‑80 (MQ=255)                                                                                                                                                                                       atttatGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTATGTTTATAAAGCAGCCAAAAAATATGTGTTATTAAACGATATTAATCTAGAAGAGTACTTTAATGAGTACCCGCCCAAGGTTTAACag                                                                                                                 <  1:116868/184‑1 (MQ=255)                                                                                                                                                                                       atttatGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTATGTTTATAAAGCAGCCAAAAAATATGTGTTATTAAACGATATTAATCTAGAAGAGTACTTTAATGAGTACCCGCCCAAGGTTTAACAGAGAAAGGGATTTTATAAATAAATGCATAAAAACATAATTATGTTTTAGTATGATGGAGTAAATATGa                                              >  1:664246/1‑251 (MQ=255)                                                                                                                                                                                                       gCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTATGTTTATAAAGCAGCCAAAAAATATGTGTTATTAAACGa                                                                                                                                                                  >  1:632557/1‑119 (MQ=255)                                                                                                                                                                                                              cAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAAc                                                                                                                                                                                                                          <  1:68466/56‑1 (MQ=255)                                                                                                                                                                                                              cAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTATGTTTATAAAGCAGCCAAAAAATATGTGTTATTAAACGATATTAATCTAGAAGAGTACTTTAATGAGTACCCGCCCAAGGTTTAACAGAGAAAGGGATTTTATAAATAAATGCATAAAAACATAAt                                                                           >  1:608582/1‑199 (MQ=255)                                                                                                                                                                                                                      ccACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTATGTTTATAAAGCAGCTAAAAAATATGTGTTATTAAACGATATTAATCTAGAAGAGTACTTTAATGAGTACCCGCCCAAGGTTTAACAGAGAAAGGGATTTTATAAATAAATGCATAAAAACATAATTATGTTTTAGTATGATGGAGTAAATATGAATATCTTAGACGAACTAAAGAAAGTATTgg                <  1:456291/250‑1 (MQ=255)                                                                                                                                                                                                                             tAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTATGTTTATAAAGCAGCCAAAAAATATGTGTTATTAAACGATATTAATCTAGAAGAGTACTTTAATGAGTACCCGCCCAAGGTTTAACAGAGAAAGGGATTTTATAAATAAATGCATAAAAACATAATTATGTTTTAGTATGATGGAGTAAATATGAATATCTTAGACGAACTAAAGAAAGTATTGGATTCAAAg        <  1:662515/251‑1 (MQ=255)                                                                                                                                                                                                                                 cAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTATGTTTATAAAGCAGCCAAAAAATATGTGTTATTAAACGATATTAATCTAGAAGAGTACTTTAATGAGTACCCGCCCAAGGTTTAACAGAGAAAGGGAt                                                                                                       <  1:440913/152‑1 (MQ=255)                                                                                                                                                                                                                                   aaaGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTATGTTTATAAAGCAGCCAAAAAATATGTGTTATTAAACGATATTAATCTAGAAGAGTACTTTAATGAGTACCCGCCCAAGGTTTAACAGAGAAAGGGATTTTATAAATAAATGCATAAAAACATAATTATGTTTTAGTATGATGGAGTAAATATGAATATCTTAGACGAACTAAAGAAAGTATTGGATTCAAAGCGTCCg  >  1:751681/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                       CTGAAAAACGACAGTAATGGCAATCAACACATAAAAACATATCTATACTAAAAAATAAATTTGTGTAACAACTTTATGCGAAATAAAATCAGAGATTAATCAAATCACTAATAAAATTTTACCGCGCTAAGAGAACAAGTATGAGTAAACTAACAAAGGTCATTAATCAGACAATCCGGGTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCCAAATAACGTACAACTATCATGAGCTCTGTTATGTTTATAAAGCAGCCAAAAAATATGTGTTATTAAACGATATTAATCTAGAAGAGTACTTTAATGAGTACCCGCCCAAGGTTTAACAGAGAAAGGGATTTTATAAATAAATGCATAAAAACATAATTATGTTTTAGTATGATGGAGTAAATATGAATATCTTAGACGAACTAAAGAAAGTATTGGATTCAAAGCGTCCG  >  1/1159618‑1160094

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑