Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,159,605	C→T	intergenic (+20475/+233706)	unknown → / ← unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,159,605	0	C	T	100.0%	89.8 / NA	34	intergenic (+20475/+233706)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/21);  total (13/21)

ACTATTGGAAAGAATCGGATATTGAAAAAACGCCTAAAGAATTTGCCTTGTTAACCAATAATCTTTTTCAGGTTGGCTAAGACCTCATCATTGGATTTTACTTTAATTTCGCCATT‑GTCAATTTTAGTGAAAAATACAACTTCTTCTGAAAG‑TGTCACTTTTTGCGGCAAATAGCCTTCCTTACGATAGGTTTCTTGTGTTGTTATGCTCATAGTTATTTTCTTTTTAAAATTTAGTCGTCATCATCATACTGAAAAACGACAGTAATGGCAATCAACACATAAAAACATATCTATACTAAAAAATAAATTTGTGTAACAACTTTATGCGAAATAAAATCAGAGATTAATCAAATCACTAATAAAATTTTACCGCGCTAAGAGAACAAGTATGAGTAAACTAACAAAGGTCATTAATCAGACAATCCGGGTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGC  >  1/1159368‑1159837                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                         aCTATTGGAAAGAATCGGATATTGAAAAAACGCCTAAAGAATTTGCCTTGTTAACCAATAATCTTTTTCAGGTTGGCTAAGACCTCATCATTGGATTTTACTTTAATTTCGCCATT‑GTCAATTTTAGTGAAAAATACAACTTCTTCTGAAAG‑TGTCACTTTTTGCGGCAAATAGCCTTCCTTACGATAGGTTTCTTGTGTTGTTATGCTCATAGTTATTTTCTTTTTAAAATTTAGTTGTCATCATCATAc                                                                                                                                                                                                                             <  1:52911/251‑1 (MQ=255)   tATTGGAAAGAATCGGATATTGAAAAAACGCCTAAAGAATTTGCCTTGTTAACCAATAATCTTTTTCAGGTTGGCTAAGACCTCATCATTGGATTTTACTTTAATTTCGCCATT‑GTCAATTTTAGTGAAAAATACAACTTCTTCTGAAAG‑TGTCACTTTTTGCGGCAAATAGCCTTCCTTACGATAGGTTTCTTGTGTTGTTATGCTCATAGTTATTTTCTTTTTAAAATTTAGTTGTCATCATCATACTg                                                                                                                                                                                                                           <  1:414185/251‑1 (MQ=255)            gAATCGGATATTGAAAAAACGCCTAAAGAATTTGCCTTGTTAACCAATAATCTTTTTCAGGTTGGCTAAGACCTCATCATTGGATTTTACTTTAATTTCGCCATT‑GTCAATTTTAGTGAAAAATACAACTTCTTCTGAAAG‑TGTCACTTTTTGCGGCAAATAGCCTTCCTTACGATAGGTTTCTTGTGTTGTTATGCTCATAGTTATTTTCTTTTTAAAATTTAGTTGTCATCATCATACTGaaa                                                                                                                                                                                                                        <  1:564046/245‑1 (MQ=255)                    tatTGAAAAAACGCCTAAAGAATTTGCCTTGTTAACCAATAATCTTTTTCAGGTTGGCTAAGACCTCATCATTGGATTTTACTTTAATTTCGCCATT‑GTCAATTTTAGTGAAAAATACAACTTCTTCTGAAAG‑TGTCACTTTTTGCGGCAAATAGCCTTCTTTACGATAGGTTTCTTGTGTTGTTATGCTCATAGTTATTTTCTTTTTAAAATTTAGTTGTCATCATCATACTGAAAAACGACAGTAATgg                                                                                                                                                                                                          <  1:850342/251‑1 (MQ=255)                            aaaCGCCTAAAGAATTTGCCTTGTTAACCAATAATCTTTTTCAGGTTGGCTAAGACCTCATCATTGGATTTTACTTTAATTTCGCCATT‑GTCAATTTTAGTGAAAAATACAACTTCTTCTGAAAG‑TGTCACTTTTTGCGGCAAATAGCCTTCCTTACGATAGGTTTCTTGTGTTGTTATGCTCATAGTTATTTTCTTTTTAAAATTTAGTTGTCATCATCATACTGAAAAACGACAGTAATGGCAATCAac                                                                                                                                                                                                  <  1:550728/251‑1 (MQ=255)                            aaaCGCCTAAAGAATTTGCCTTGTTAACCAATAATCTTTTTCAGGTTGGCTAAGACCTCATCATTGGATTTTACTTTAATTTCGCCATT‑GTCAATTTTAGTGAAAAATACAACTTCTTCTGAAAG‑TGTCACTTTTTGCGGCAAATAGCCTTCCTTACGATAGGTTTCTTGTGTTGTTATGCTCATAGTTATTTTCTTTTTAAAATTTAGTTGTCATCATCATACTGAAAAACGACAGTAATGGCAATCAac                                                                                                                                                                                                  <  1:266396/251‑1 (MQ=255)                               cGCCTAAAGAATTTGCCTTGTTAACCAATAATCTTTTTCAGGTTGGCTAAGACCTCATCATTGGATTTTACTTTAATTTCGCCATT‑GTCAATTTTAGTGAAAAATACAACTTCTTCTGAAAG‑TGTCACTTTTTGCGGCAAATAGCCTTCCTTACGATAGGTTTCTTGTGTTGTTATGCTCATAGTTATTTTCTTTTTAAAATTTAGTTGTCATCATCATACTGAAAAACGACAGTAATGGCAATCAAcaca                                                                                                                                                                                               >  1:633650/1‑251 (MQ=255)                                   tAAAGAATTTGCCTTGTTAACCAATAATCTTTTTCAGGTTGGCTAAGTCCTCATCATTGGATTTTACTTTAATTTCGCCATT‑GTCAATTTTAGTGAAAAATACAACTTCTTCTGAAAG‑TGTCACTTTTTGCGGCAAATAGCCTTCCTTACGATAGGTTTCTTGTGTTGTTATGCTCATAGTTATTTTCTTTTTAAAATTTAGTTGTCATCATCATACTGAAAAACGACAGTAATGGCAATCAACACATaaa                                                                                                                                                                                           <  1:94508/251‑1 (MQ=255)                                     aaGAATTTGCCTTTTTAACCAATATTCTTTTTAAGGTTGGTAAAGACCTAATAATTGAATTTTACTTTAATTTCG‑CATTGGTCAATTTTAGTAAAAAATACACCTTCTTCT‑AAAGTTGTCACTTTTGGGGGCAATTGGCCTTCCTTCCGAAAGGTTTCTTGTGTGGTTATCCTCAAAGTTA‑TTTCCTTTTAAAATTTAGTTGTCATCATCATACTGAAAAACGACAGTAATGGCAATCAACACATAAAAAc                                                                                                                                                                                        <  1:182205/251‑1 (MQ=255)                                        aaTTTGCCTTGTTAACCAATAATCTTTTTCAGGTTGGCTAAGACCTCATCATTGGATTTTACTTTAATTTCGCCATT‑GTCAATTTTAGTGAAAAATACAACTTCTTCTGAAAG‑TGTCACTTTTTGCGGCAAATAGCCTTCCTTACGATAGGTTTCTTGTGTTGTTATGCTCATAGTTATTTTCTTTTTAAAATTTAGTTGTCATCATCATACTGAAAAACGACAGTAATGGCAATCAACACATAAAAAc                                                                                                                                                                                        <  1:773100/249‑1 (MQ=255)                                        aaTTTGCCTTGTTAACCAATAATCTTTTTCAGGTTGGCTAAGACCTCATCATTGGATTTTACTTTAATTTCGCCATT‑GTCAATTTTAGTGAAAAATACAACTTCTTCTGAAAG‑TGTCACTTTTTGCGGCAAATAGCCTTCCTTACGATAGGTTTCTTGTGTTGTTATGCTCATAGTTATTTTCTTTTTAAAATTTAGTTGTCATCATCATACTGAAAAACGACAGTAATGGCAATCAACACATAAAAAc                                                                                                                                                                                        <  1:64744/249‑1 (MQ=255)                                           ttGCCTTGTTAACCAATAATCTTTTTCAGGTTGGCTAAGACCTCATCATTGGATTTTACTTTAATTTCGCCATT‑GTCAATTTTAGTGAAAAATACAACTTCTTCTGAAAG‑TGTCGCTTTTTGCGGCAAATAGCCTTCCTTACGATAGGTTTCTTGTGTTGTTATGCTCATAGTTATTTTCTTTTTAAAATTTAGTTGTCATCATCATACTGAAAAACGACAGTAATGGCAATCAACGCATAAAAACATATc                                                                                                                                                                                   <  1:42160/251‑1 (MQ=255)                                           ttGCCTTGTTAACCAATAATCTTTTTCAGGTTGGCTAAGACCTCATCATTGGATTTTACTTTAATTTCGCCATT‑GTCAATTTTAGTGAAAAATACAACTTCTTCTGAAAG‑TGTCACTTTTTGCGGCAAATAGCCTTCCTTACGATAGGTTTCTTGTGTTGTTATGCTCATAGTTATTTTCTTTTTAAAATTTAGTTGTCATCATCATACTGAAAAACGACAGTAATGGCAATCAACACATAAAAACATATc                                                                                                                                                                                   <  1:332834/251‑1 (MQ=255)                                           ttGCCTTGTTAACCAATAATCTTTTTCAGGTTGGCTAAGACCTCATCATTGGATTTTACTTTAATTTCGCCATT‑GTCAATTTTAGTGAAAAATACAACTTCTTCTGAAAG‑TGTCACTTTTTGCGGCAAATAGCCTTCCTTACGATAGGTTTCTTGTGTTGTTATGCTCATAGTTATTTTCTTTTTAAAATTTAGTTGTCATCATCATACTGAAAAACGACAGTAATGGCAATCAACACATAAAAACATATc                                                                                                                                                                                   <  1:143188/251‑1 (MQ=255)                                              ccTTGTTAACCAATAATCTTTTTCAGGTTGGCTAAGACCTCATCATTGGATTTTACTTTAATTTCGCCATT‑GTCAATTTTAGTGAAAAATACAACTTCTTCTGAAAG‑TGTCACTTTTTGCGGCAAATAGCCTTCCTTACGATAGGTTTCTTGTGTTGTTATGCTCATAGTTATTTTCTTTTTAAAATTTAGTTGTCATCATCATACTGAAAAACGACAGTAATGGCAATCAACACATAAAAACGTATCtat                                                                                                                                                                                >  1:449154/1‑251 (MQ=255)                                                  gTTAACCAATAATCTTTTTCAGGTTGGCTAAGACCTCATCATTGGATTTTACTTTAATTTCGCCATT‑GTCAATTTTAGTGAAAAATACAACTTCTTCTGAAAG‑TGTCACTTTTTGCGGCAAATAGCCTTCCTTACGATAGGTTTCTTGTGTTGTTATGCTCATAGTTATTTTCTTTTTAAAATTTAGTTGTCATCATCATACTGAAAAACGACAGTAATGGCAATCAACACATAAAAACATATc                                                                                                                                                                                   >  1:884205/1‑244 (MQ=255)                                                       ccAATAATCTTTTTCAGGTTGGCTAAGACCTCATCATTGGATTTTACTTTAATTTCGCCATT‑GTCAATTTTAGTGAAAAATACAACTTCTTCTGAAAG‑TGTCACTTTTTGCGGCAAATAGCCTTCCTTACGATAGGTTTCTTGTGTTGTTATGCTCATAGTTATTTTCTTTTTAAAATTTAGTTGTCATCATCATACTGAAAAACGACAGTAATGGCAATCAACACATAAAAAc                                                                                                                                                                                        >  1:696604/1‑234 (MQ=255)                                                             atCTTTTTCAGGTTGGCTAAGACCTCATCATTGGATTTTACTTTAATTTCGCCATT‑GTCAATTTTAGTGAAAAATACAACTTCTTCTGAAAG‑TGTCACTTTTTGCGGCAAATAGCCTTCCTTACGATAGGTTTCTTGTGTTGTTATGCTCATAGTTATTTTCTTTTTAAAATTTAGTTGTCATCATCATACTGAAAAACGACAGTAATGGCAATCAACACATAAAAACATATCTATACTAAAAAATAAAtt                                                                                                                                                                 <  1:303982/251‑1 (MQ=255)                                                                           ggCTAAGACCTCATCATTGGATTTTACTTTAATTTCGCCATT‑GTCAATTTTAGTGAAAAATACAACTTCTTCTGAAAG‑TGTCACTTTTTGCGGCAAATAGCCTTCCTTACGATAGGTTTCTTGTGTTGTTATGCTCATAGTTATTTTCTTTTTAAAATTTAGTTGTCATCATCATACTGAAAAACGACAGTAATGGCAATCAACACATAAAAACATATCTATACTAAAAAATAAATTTGTGTAACAACttt                                                                                                                                                   >  1:228904/1‑251 (MQ=255)                                                                            gCTAAGACCTCATCATTGGATTTTACTTTAATTTCGCCATT‑GTCAATTTTAGTGAAAAATACAACTTCTTCTGAAAG‑TGTCACTTTTTGCGGCAAATAGCCTTCCTTACGATAGGTTTCTTGTGTTGTTATGCTCATAGTTATTTTCTTTTTAAAATTTAGTTGTCATCATCATACTGAAAAACGACAGTAATGGCAATCAACACATAAAAACATATCTATACTaaaa                                                                                                                                                                         <  1:125148/228‑1 (MQ=255)                                                                                        tcatTGGATTTTACTTTAATTTCGCCATT‑GTCAATTTTAGTGAAAAATACAACTTCTTCTGAAAG‑TGTCACTTTTTGCGGCAAATAGCCTTCCTTACGATAGGTTTCTTGTGTTGTTATGCTCATAGTTATTTTCTTTTTAAAATTTAGTTGTCATCATCATACTGAAAAACGACAGTAATGGCAATCAac                                                                                                                                                                                                  >  1:634533/1‑191 (MQ=255)                                                                                            tGGATTTTACTTTAATTTCGCCATT‑GTCAATTTTAGTGAAAAATACAACTTCTTCTGAAAG‑TGTCACTTTTTGCGGCAAATAGCCTTCCTTACGATAGGTTTCTTGTGTTGTTATGCTCATAGTTATTTTCTTTTTAAAATTTAGTTGTCATCATCATACTGAAAAACGACAGTAATGGCAATCAACACATAAAAACATATCTATACTAAAAAATAAATTTGTGTAACAACTTTATGCGAAATAAAATCag                                                                                                                                  <  1:501393/251‑1 (MQ=255)                                                                                              gATTTTACTTTAATTTCGCCATT‑GTCAATTTTAGTGAAAAATACAACTTCTTCTGAAAG‑TGTCACTTTTTGCGGCAGATAGCCTTCCTTACGATAGGTTTCTTGTGTTGTTATGCTCATAGTTATTTTCTTTTTAAAATTTAGTTGTCATCATCATACTGAAAAACGACAGTAATGGCAATCAACACATAAAAACATATCTATACTAAAAAATAAATTTGTGTAACAACTTTATGCGAAATAAAATCagag                                                                                                                                >  1:104212/1‑251 (MQ=255)                                                                                                    aCTTTAATTTCGCCATT‑GTCAATTTTAGTGAAAAATACAACTTCTTCTGAAAG‑TGTCACTTTTTGCGGCAAATAGCCTTCCTTACGATAGGTTTCTTGTGTTGTTATGCTCATAGTTATTTTCTTTTTAAAATTTAGTTGTCATCATCATACTGAAAAACGACAGTAATGGCAATCAACACATAAAAACATATCTATAc                                                                                                                                                                              >  1:104093/1‑199 (MQ=255)                                                                                                                                       aTACAACTTCTTCTGAAAG‑TGTCACTTTTTGCGGCAAATAGCCTTCCTTACGATAGGTTTCTTGTGTTGTTATGCTCATAGTTATTTTCTTTTTAAAATTTAGTTGTCATCATCATACTGAAAAACGACAGTAATGGCAATCAACACATAAAAACATATCTATACTAAAAAATAAATTTGTGTAACAACTTTATGCGAAATAAAATCAGAGATTAATCAAATCACTAATAAAATTTTACCGCGCTAAgaga                                                                                        >  1:760479/1‑251 (MQ=255)                                                                                                                                         aCAACTTCTTCTGAAAG‑TGTCACTTTTTGCGGCAAATAGCCTTCCTTACGATAGGTTTCTTGTGTTGTTATGCTCATAGTTATTTTCTTTTTAAAATTTAGTTGTCATCATCATACTGAAAAACGACAGTAATGGCAATCAACACATAAAAACATATCTATACTAAAAAATAAATTTGTGTAACAACTTTATGCGAAATAAAATCAGAGATTAATCAAATCACTAATAAAATTTTACCGCGCTAAGAGAAc                                                                                      <  1:177594/251‑1 (MQ=255)                                                                                                                                                                                        tACGATAGGTTTCTTGTGTTGTTATGCTCATAGTTATTTTCTTTTTAAAATTTAGTTGTCATCATCATACTGAAAAACGACAGTAATGGCAATCAACACATAAAAAc                                                                                                                                                                                        >  1:500015/1‑107 (MQ=255)                                                                                                                                                                                         aCGATAGGTTTCTTGTGTTGTTATGCTCATAGTTATTTTCTTTTTAAAATTTAGTTGTCATCATCATACTGAAAAACGACAGTAATgg                                                                                                                                                                                                          <  1:235863/88‑1 (MQ=255)                                                                                                                                                                                                 tttCTTGTGTTGTTATGCTCATAGTTATTTTCTTTTTAAAATTTAGTTGTCATCATCATACTGAAAAACGACAGTAATGGCAATCAACACATAAAAACATATCTATACTAAAAAATAAATTTGTGTAACAACTTTATGCGAAATAAAATCAGAGATTAATCAAATCACTAATAAAATTTTACCGCGCTAAGAGAACAAGTATGAGTAAACTAACAAAGGTCATTAATCAGACAATCCGGGTTATTTATggg                               <  1:185794/251‑1 (MQ=255)                                                                                                                                                                                                  ttCTTGTGTTGTTATGCTCATAGTTATTTTCTTTTTAAAATTTAGTTGTCATCATCATACTGAAAAACGACAGTAATGGCAATCAACACATAAAAACATATCTATACTAAAAAATAAATTTGTGTAACAACTTTATGCGAAATAAAATCAGAGATTAATCAAATCACTAATAAAATTTTACCGCGCTAAGAGAACAAGTATGAGTAAACTAACAAAGGTCATTAATCAGACAATCCGGGTTATTTATGGGa                              >  1:657816/1‑251 (MQ=255)                                                                                                                                                                                                            gttATGCTCATAGTTATTTTCTTTTTAAAATTTAGTTGTCATCATCATACTGAAAAACGACAGTAATGGCAATCAACACATAAAAACATATCTATACTAAAAAATAAATTTGTGTAACAACTTTATGCGAAATAAAATCAGAGATTAATCAAATCACTAATAAAATTTTACCGCGCTAAGAGAACAAGTATGAGTAAACTAACAAAGGTCATTAATCAGACAATCCGGGTTATTTATGGGAACGATTGCaa                    >  1:304642/1‑251 (MQ=255)                                                                                                                                                                                                                 gCTCATAGTTATTTTCTTTTTAAAATTTAGTTGTCATCATCATACTGAAAAACGACAGTAATGGCAATCAACACATAAAAACATATCTATACTAAAAAATAAATTTGTGTAACAACTTTATGCGAAATAAAATCAGAGATTAATCAAATCACTAATAAAATTTTACCGCGCTAAGAGAACAAGTATGAGTAAACTAACAAAGGTCATTAATCAGACAATCCGGGTTATTTATGGGAACGATTGCAAAAACa               >  1:90477/1‑251 (MQ=255)                                                                                                                                                                                                                        gTTATTTTCTTTTTAAAATTTAGTTGTCATCATCATACTGAAAAACGACAGTAATGGCAATCAACACATAAAAACATATCTATACTAAAAAATAAATTTGTGTAACAACTTTATGCGAAATAAAATCAGAGATTAATCAAATCACTAATAAAATTTTACCGCGCTAAGAGAACAAGTATGAGTAAACTAACaa                                                                  <  1:229128/193‑1 (MQ=255)                                                                                                                                                                                                                              ttCTTTTTAAAATTTAGTTGTCATCATCATACTGAAAAACGACAGTAATGGCAATCAACACATAAAAACATATCTATACTAAAAAATAAATTTGTGTAACAACTTTATGCGAAATAAAATCAGAGATTAATCAAATCACTAATAAAATTTTACCGCGCTAAGAGAACAAGTATGAGTAAACTAACAAAGGTCATTAATCAGACAATCCGGGTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGc  <  1:798506/251‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                         ACTATTGGAAAGAATCGGATATTGAAAAAACGCCTAAAGAATTTGCCTTGTTAACCAATAATCTTTTTCAGGTTGGCTAAGACCTCATCATTGGATTTTACTTTAATTTCGCCATT‑GTCAATTTTAGTGAAAAATACAACTTCTTCTGAAAG‑TGTCACTTTTTGCGGCAAATAGCCTTCCTTACGATAGGTTTCTTGTGTTGTTATGCTCATAGTTATTTTCTTTTTAAAATTTAGTCGTCATCATCATACTGAAAAACGACAGTAATGGCAATCAACACATAAAAACATATCTATACTAAAAAATAAATTTGTGTAACAACTTTATGCGAAATAAAATCAGAGATTAATCAAATCACTAATAAAATTTTACCGCGCTAAGAGAACAAGTATGAGTAAACTAACAAAGGTCATTAATCAGACAATCCGGGTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGC  >  1/1159368‑1159837

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑