Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,156,958	A→G	intergenic (+17828/+236353)	unknown → / ← unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,156,958	0	A	G	100.0%	223.1 / NA	78	intergenic (+17828/+236353)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (42/36);  total (42/36)

CGGCGACGGTTATGCGAAAGTGATGTCCGATGAGTTCAATCGTCGCGCGTTAGAACTTTACGCCGAACAAGCGAAAGAAGTGGATATTATTATTACCACCGCGTTAATTCCGGGCAAACCGGCGCCACGCTTGATCACCAAAGAAATGGTGGCATCCATGAAACCGGGTAGCGTAATCGTGGATTTGGCAGCAGCCACCGGCGGTAACTGTGAACTCAGCAAAGCAGGCAAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTGATTTGCCAAGCCGTTTACCAACCCAATCTTCACAGCTTTACGGCACCAACCTTGTTAATTTATTGAAATTACTTTGTAAAAACAAAGACGGTCAAATTGACATCAATTTTGATGATGTAGTATTGCGTGGCGTGACTGTGATTCGTGACGGTGAAATTACTTGGCCGGCACCACCGATTCAGGTTTCCGCTCAGCCGCAG  >  1/1156729‑1157203                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      cggctACGGTTATGCGAAAGTGATGTCCGATGAGTTCAATCGTCGCGCGTTAGAACTTTACGCCGAACAAGCGAAAGAAGTGGATATTATTATTACCACCGCGTTAATTCCGGGCAAACCGGCGCCACGCTTGATCACCAAAGAAATGGTGGCATCCATGAAACCGGGTAGCGTAATCGTGGATTTGGCAGCAGCCACCGGCGGTAACTGTGAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAAc                                                                                                                                                                                                                                   >  1:762414/1‑250 (MQ=255) cggcgACGGTTATGCGAAAGTGATGTCCGATGAGTTCAATCGTCGCGCGTTAGAACTTTACGCCGAACAAGCGAAAGAAGTGGATATTATTATTACCACCGCGTTAATTCCGGGCAAACCGGCGCCACGCTTGATCACCAAAGAAATGGTGGCATCCATGAAACCGGGTAGCGTAATCGTGGATTTGGCAGCAGCCACCGGCGGTAACTGTGAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAAc                                                                                                                                                                                                                                   <  1:251691/250‑1 (MQ=255)  ggcgACGGTTATGCGAAAGTGATGTCCGATGAGTTCAATCGTCGCGCGTTAGAACTTTACGCCGAACAAGCGAAAGAAGTGGATATTATTATTACCACCGCGTTAATTCCGGGCAAACCGGCGCCACGCTTGATCACCAAAGAAATGGTGGCATCCATGAAACCGGGTAGCGTAATCGTGGATTTGGCAGCAGCCACCGGCGGTAACTGTGAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAACCa                                                                                                                                                                                                                                 >  1:624302/1‑251 (MQ=255)       cGGTTATGCGAAAGTGATGTCCGATGAGTTCAATCGTCGCGCGTTAGAACTTTACGCCGAACAAGCGAAAGAAGTGGATATTATTATTACCACCGCGTTAATTCCGGGCAAACCGGCGCCACGCTTGATCACCAAAGAAATGGTGGCATCCATGAAACCGGGTAGCGTAATCGTGGATTTGGCAGCAGCCACCGGCGGTAACTGTGAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAACCAAGTGa                                                                                                                                                                                                                            >  1:172278/1‑251 (MQ=255)        ggTTATGCGAAAGTGATGTCCGATGAGTTCAATCGTCGCGCGTTAGAACTTTACGCCGAACAAGCGAAAGAAGTGGATATTATTATTACCACCGCGTTAATTCCGGGCAAACCGGCGCCACGCTTGATCACCAAAGAAATGGTGGCATCCATGAAACCGGGTAGCGTAATCGTGGATTTGGCAGCAGCCACCGGCGGTAACTGTGAACTCAGCAAAGCAGGCGAAGGGGTGGTTACCGACAACCAAGTGaa                                                                                                                                                                                                                           >  1:770975/1‑251 (MQ=255)         gTTATGCGAAAGTGATGTCCGATGAGTTCAATCGTCGCGCGTTAGAACTTTACGCCGAACAAGCGAAAGAAGTGGATATTATTATTACCACCGCGTTAATTCCGGGCAAACCGGCGCCACGCTTGATCACCAAAGAAATGGTGGCATCCATGAAACCGGGTAGCGTAATCGTGGATTTGGCAGCAGCCACCGGCGGTAACTGTGAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAACCAAGTGaaa                                                                                                                                                                                                                          >  1:805316/1‑251 (MQ=255)                                gAGTTCAATCGTCGCGCGTTAGAACTTTACGCCGAACAAGCGAAAGAAGTGGATATTATTATTACCACCGCGTTAATTCCGGGCAAACCGGCGCCACGCTTGATCACCAAAGAAATGGTGGCATCCATGAAACCGGGTAGCGTAATCGTGGATTTGGCAGCAGCCACCGGCGGTAACTGTGAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTg                                                                                                                                                                                                          <  1:539667/244‑1 (MQ=255)                                  gTTCAATCGTCGCGCGTTAGAACTTTACGCCGAACAAGCGAAAGAAGTGGATATTATTATTACCACCGCGTTAATTCCGGGCAAACCGGCGCCACGCTTGATCACCAAAGAAATGGTGGCATCCATGAAACCGGGTAGCGTAATCGTGGATTTGGCAGCAGCCACCGGCGGTAACTGTGAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTGATTTGCCaa                                                                                                                                                                                                 >  1:461244/1‑251 (MQ=255)                                     cAATCGTCGCGCGTTAGAACTTTACGCCGAACAAGCGAAAGAAGTGGATATTATTATTACCACCGCGTTAATTCCGGGCAAACCGGCGCCACGCTTGATCACCAAAGAAATGGTGGCATCCATGAAACCGGGTAGCGTAATCGTGGATTTGGCAGCAGCCACCGGCGGTAACTGTGAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTGATTTGCCAAGcc                                                                                                                                                                                              <  1:820233/251‑1 (MQ=255)                                            cgcgcgTTAGAACTTTACGCCGAACAAGCGAAAGAAGTGGATATTATTATTACCACCGCGTTAATTCCGGGCAAACCGGCGCCACGCTTGATCACCAAAGAAATGGTGGCATCCATGAAACCGGGTAGCGTAATCGTGGATTTGGCAGCAGCCACCGGCGGTAACTGTGAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTGATTTGCCAAGCCGTTTAcc                                                                                                                                                                                       <  1:149699/251‑1 (MQ=255)                                                             cGCCGAACAAGCGAAAGAAGTGGATATTATTATTACCACCGCGTTAATTCCGGGCAAACCGGCGCCACGCTTGATCACCAAAGAAATGGTGGCATCCATGAAACCGGGTAGCGTAATCGTGGATTTGGCAGCAGCCACCGGCGGTAACTGTGAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTGATTTGCCAAGCCGTTTACCAACCCAATCTTCACAGc                                                                                                                                                                      <  1:66759/251‑1 (MQ=255)                                                                 gAACAAGCGAAAGAAGTGGATATTATTATTACCACCGCGTTAATTCCGGGCAAACCGGCGCCACGCTTGATCACCAAAGAAATGGTGGCATCCATGAAACCGGGTAGCGTAATCGTGGATTTGGCAGCAGCCACCGGCGGTAACTGTGAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTGATTTGCCAAGCCGTTTACCAACCCAATCTTCACAGCTTTa                                                                                                                                                                  >  1:564276/1‑251 (MQ=255)                                                                  aaCAAGCGAAAGAAGTGGATATTATTATTACCACCGCGTTAATTCCGGGCAAACCGGCGCCACGCTTGATCACCAAAGAAATGGTGGCATCCATGAAACCGGGTAGCGTAATCGTGGATTTGGCAGCAGCCACCGGCGGTAACTGTGAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTGATTTGCCAAGCCGTTTACCAACCCAATCTTCACAGCTTTAc                                                                                                                                                                 <  1:647667/251‑1 (MQ=255)                                                                    cAAGCGAAAGAAGTGGATATTATTATTACCACCGCGTTAATTCCGGGCAAACCGGCGCCACGCTTGATCACCAAAGAAATGGTGGCATCCATGAAACCGGGTAGCGTAATCGTGGATTTGGCAGCAGCCACCGGCGGTAACTGTGAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTGATTTGCCAAGCCGTTTACCAACCCAATCTTCACAGCTTTACgg                                                                                                                                                               <  1:386674/251‑1 (MQ=255)                                                                        cGAAAGAAGTGGATATTATTATTACCACCGCGTTAATTCCGGGCAAACCGGCGCCACGCTTGATCACCAAAGAAATGGTGGCATCCATGAAACCGGGTAGCGTAATCGTGGATTTGGCAGCAGCCACCGGCGGTAACTGTGAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTGATTTGCCAAGCCGTTTACCAACCCAATCTTCACAGCTTTACGGCAc                                                                                                                                                            <  1:585720/250‑1 (MQ=255)                                                                             gaagTGGATATTATTATTACCACCGCGTTAATTCCGGGCAAACCGGCGCCACGCTTGATCACCAAAGAAATGGTGGCATCCATGAAACCGGGTAGCGTAATCGTGGATTTGGCAGCAGCCACCGGCGGTAACTGTGAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTacac                                                                                                                                                                                                            >  1:779707/1‑197 (MQ=255)                                                                                  ggATATTATTATTACCACCGCGTTAATTCCGGGCAAACCGGCGCCACGCTTGATCACCAAAGAAATGGTGGCATCCATGAAACCGGGTAGCGTAATCGTGGATTTGGCAGCAGCCACCGGCGGTAACCGTGAACTCAACAAAAAAGGCGAAGAGGTGGCTACCGACaaac                                                                                                                                                                                                                                  >  1:576870/1‑168 (MQ=255)                                                                                    atatTATTATTACCACCGCGTTAATTCCGGGCAAACCGGCGCCACGCTTGATCACCAAAGAAATGGTGGCATCCATGAAACCGGGTAGCGTAATCGTGGATTTGGCAGCAGCCACCGGCGGTAACTGTGAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTGATTTGCCAAGCCGTTTACCAACCCAATCTTCACAGCTTTACGGCACCAACCTTGTTAAt                                                                                                                                               <  1:407624/251‑1 (MQ=255)                                                                                    atatTATTATTACCACCGCGTTAATTCCGGGCAAACCGGCGCCACGCTTGATCACCAAAGAAATGGTGGCATCCATGAAACCGGGTAGCGTAATCGTGGATTTGGCAGCAGCCACCGGCGGTAACTGTGAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACAGATTTGCCAAGCCGTTTACCAACCCAATCTTCACAGCTTTACGGCACCAACCCTGTTAAt                                                                                                                                               >  1:388517/1‑251 (MQ=255)                                                                                    atatTATTATTACCACCGCGTTAATTCCGGGCAAACCGGCGCCACGCTTGATCACCAAAGAAATGGTGGCATCCATGAAACCGGGTAGCGTAATCGTGGATTTGGCAGCAGCCACCGGCGGTAACTGTGAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAACCAAGGGAAAATCATCGGGTACACTGATTTGCCAAGCCg                                                                                                                                                                                             >  1:343248/1‑205 (MQ=255)                                                                                       ttattattaCCACCGCGTTAATTCCGGGCAAACCGGCGCCACGCTTGATCACCAAAGAAATGGTGGCATCCATGAAACCGGGTAGCGTAATCGTGGATTTGGCAGCAGCCACCGGCGGTAACTGTGAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTGATTTGCCAAGCCGTTTACCAACCCAATCTTCACAGCTTTACGGCACCAACCTTGTTAATTTa                                                                                                                                            >  1:325652/1‑251 (MQ=255)                                                                                         attattaCCACCGCGTTAATTCCGGGCAAACCGGCGCCACGCTTGATCACCAAAGAAATGGTGGCATCCATGAAACCGGGTAGCGTAATCGTGGATTTGGCAGCAGCCACCGGCGGTAACTGTGAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTGATTTGCCAAGCCGTTTACCAACCCAATCTTCACAGCTTTACGGCACCAACCTTGTTaa                                                                                                                                                <  1:816045/245‑1 (MQ=255)                                                                                         attattaCCACCGCGTTAATTCCGGGCAAACCGGCGCCACGCTTGATCACCAAAGAAATGGTGGCATCCATGAAACCGGGTAGCGTAATCGTGGATTTGGCAGCAGCCACCGGCGGTAACTGTGAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTGATTTGCCAAGCCGTTTACCAACCCAATCTTCACAGCTTTACGGCACCAACCTTGTTAATTTAtt                                                                                                                                          >  1:732756/1‑251 (MQ=255)                                                                                         attattaCCACCGCGTTAATTCCGGGCAAACCGGCGCCACGCTTGATCACCAAAGAAATGGTGGCATCCATGAAACCGGGTAGCGTAATCGTGGATTTGGCAGCAGCCACCGGCGGTAACTGTGAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTGATTTGCCAAGCCGTTTACCAACCCAATCTTCACAGCTTTACGGCACCAACCTTGTTAATTTAtt                                                                                                                                          >  1:376916/1‑251 (MQ=255)                                                                                          ttattaCCACCGCGTTAATTCCGGGCAAACCGGCGCCACGCTTGATCACCAAAGAAATGGTGGCATCCATGAAACCGGGTAGCGTAATCGTGGATTTGGCAGCAGCCACCGGCGGTAACTGTGAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTGATTTGCCAAGCCGTTTACCAACCCAATCTTCACAGCTTTACGGCACCAACCTTGTTAATTTATTg                                                                                                                                         >  1:838698/1‑251 (MQ=255)                                                                                          ttattaCCACCGCGTTAATTCCGGGCAAACCGGCGCCACGCTTGATCACCAAAGAAATGGTGGCATCCATGAAACCGGGTAGCGTAATCGTGGATTTGGCAGCAGCCACCGGCGGTAACTGTGAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTGATTTGCCAAGCCGTTTACCAACCCAATCTTCACAGCTTTACGGCACCAACCTTGTTAATTTATTg                                                                                                                                         <  1:853278/251‑1 (MQ=255)                                                                                                   ccGCGTTAATTCCGGGCAAACCGGCGCCACGCTTGATCACCAAAGAAATGGTGGCATCCATGAAACCGGGTAGCGTAATCGTGGATTTGGCAGCAGCCACCGGCGGTAACTGTGAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTGATTTGCCAAGCCGTTTACCAACCCAATCTTCACAGCTTTACGGCACCAACCTTGTTAATTTATTGAAATTACtt                                                                                                                                <  1:356788/251‑1 (MQ=255)                                                                                                     gcgTTAATTCCGGGCAAACCGGCGCCACGCTTGATCACCAAAGAAATGGTGGCATCCATGAAACCGGGTAGCGTAATCGTGGATTTGGCAGCAGCCACCGGCGGTAACTGTGAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTGATTTGCCAAGCCGTTTACCAACCCAATCTTCACAGCTTTACgg                                                                                                                                                               <  1:191646/218‑1 (MQ=255)                                                                                                           aTTCCGGGCAAACCGGCGCCACGCTTGATCACCAAAGAAATGGTGGCATCCATGAAACCGGGTAGCGTAATCGTGGATTTGGCAGCAGCCACCGGCGGTAACTGTGAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTGATTTGCCAAGCCGTTTACCAACCCAATCTTCACAGCTTTACGGCACCAACCTTGTTAATTTATTGAAATTACTTTGTaaaaa                                                                                                                        >  1:734692/1‑251 (MQ=255)                                                                                                           aTTCCGGGCAAACCGGCGCCACGCTTGATCACCAAAGAAATGGTGGCATCCATGAAACCGGGTAGCGTAATCGTGGATTTGGCAGCAGCCACCGGCGGTAACTGTGAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTGATTTGCCAAGCCGTTTACCAACCCAATCTTCACAGCTTTACGGCACCAACCTTGTTAATTTATTGAAATTACTTTGTaaaaa                                                                                                                        >  1:136138/1‑251 (MQ=255)                                                                                                                gggCAAACCGGCGCCACGCTTGATCACCAAAGAAATGGTGGCATCCATGAAACCGGGTAGCGTAATCGTGGATTTGGCAGCAGCCACCGGCGGTAACTGTGAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTGATTTGCCAAGCCGTTTACCAAccc                                                                                                                                                                                  >  1:272933/1‑188 (MQ=255)                                                                                                                     aaCCGGCGCCACGCTTGATCACCAAAGAAATGGTGGCATCCATGAAACCGGGTAGCGTAATCGTGGATTTGGCAGCAGCCACCGGCGGTAACTGTGAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAAc                                                                                                                                                                                                                                   >  1:857156/1‑134 (MQ=255)                                                                                                                             ccACGCTTGATCACCAAAGAAATGGTGGCATCCATGAAACCGGGTAGCGTAATCGTGGATTTGGCAGCAGCCACCGGCGGTAACTGTGAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTGATTTGCCAAGCCGTTTACCAACCCAATCTTCACAGCTTTACGGCACCAACCTTGTTAATTTATTGAAATTACTTTGTAAAAACAAAGACGGTCAAATTg                                                                                                       <  1:407600/250‑1 (MQ=255)                                                                                                                                cGCTTGATCACCAAAGAAATGGTGGCATCCATGAAACCGGGTAGCGTAATCGTGGATTTGGCAGCAGCCACCGGCGGTAACTGTGAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTGATTTGCCAAGCCGTTTACCAACCCAATCTTCACAGCTTTACGGCACCAAcc                                                                                                                                                       >  1:659335/1‑199 (MQ=255)                                                                                                                                cGCTTGATCACCAAAGAAATGGTGGCATCCATGAAACCGGGTAGCGTAATCGTGGATTTGGCAGCAGCCACCGGCGGTAACTGTGAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTGATTTGCCAAGCCGTTTACCAACCCAATCTTCACAGCTTTACGGCACCAACCTTGTTAATTTATTGAAATTACTTTGTAAAAACAAAGACGGTCAAATTGACAt                                                                                                   >  1:744186/1‑251 (MQ=255)                                                                                                                                  cTTGATCACCAAAGAAATGGTGGCATCCATGAAACCGGGTAGCGTAATCGTGGATTTGGCAGCAGCCACCGGCGGTAACTGTGAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTGATTTGCCAAGCCGTTTACCAACCCAATCTTCACAGCTTTACGGCACCAACCt                                                                                                                                                      <  1:536248/198‑1 (MQ=255)                                                                                                                                  cTTGATCACCAAAGAAATGGTGGCATCCATGAAACCGGGTAGCGTAATCGTGGATTTGGCAGCAGCCACCGGCGGTAACTGTGAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTGATTTGCCAAGCCGCTTACCAACCCAATCTTCACAGCTTTACGGCACCAACCTTGTTAATTTATTGAAATTACTTTGTAAAAACAAAGACGGTCAAATTGACATCa                                                                                                 >  1:246377/1‑251 (MQ=255)                                                                                                                                      aTCACCAAAGAAATGGTGGCATCCATGAAACCGGGTAGCGTAATCGTGGATTTGGCAGCAGCCACCGGCGGTAACTGTGAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTGATTTGCCAAGCCGTTTACCAACCCAATCTTCACAGCTTTACGGCACCAACCTTGTTAATTTATTGAAATTACTTTGTAAAAACAAAGACGGTCAAATTGACATCAAttt                                                                                             <  1:603462/251‑1 (MQ=255)                                                                                                                                        cACCAAAGAAATGGTGGCATCCATGAAACCGGGTAGCGTAATCGTGGATTTGGCAGCAGCCACCGGCGGTAACTGTGAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTGATTTGCCAAGCCGTTTACCAACCCAATCTTCACAGCTTTACGGCACCAACCTTGTTAAttt                                                                                                                                             >  1:61726/1‑201 (MQ=255)                                                                                                                                          ccAAAGAAATGGTGGCATCCATGAAACCGGGTAGCGTAATCGTGGATTTGGCAGCAGCCACCGGCGGTAACTGTGAACTCAGCAAAGCAGGCGACGAGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTGATTTGCCAAGCCGCTTACCCACCCAATCTTCACAGCTTTACGGCACCAACCTTGTTAATTTATTGAAATTACTTTGTAAAAACAAAGACCGTCAAATTGCCATCAATTTtgat                                                                                         >  1:48272/1‑251 (MQ=255)                                                                                                                                          ccAAAGAAATGGTGGCATCCATGAAACCGGGTAGCGTAATCGTGGATTTGGCAGCAGCCACCGGCGGTAACTGTGAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTGATTTGCCAAGCCGTTTACCAACCCAATCTTCACAGCTTTACGGCACCAAcc                                                                                                                                                       <  1:215164/189‑1 (MQ=255)                                                                                                                                          ccAAAGAAATGGTGGCATCCATGAAACCGGGTAGCGTAATCGTGGATTTGGCAGCAGCCACCGGCGGTAACTGTGAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTGATTTGCCAAGCCGTTTACCAACCCAATCTTCACAGCTTTACGGCACCAACCTTGTTAATTTATTGAAATTACTTTGTAAAAACAAAGACGGTCAAATTGACATCAATTTtgat                                                                                         >  1:899079/1‑251 (MQ=255)                                                                                                                                           cAAAGAAATGGTGGCATCCATGAAACCGGGTAGCGTAATCGTGGATTTGGCAGCAGCCACCGGCGGTAACTGTGAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTGATTTGCCAAGCCGTTTACCAACCCAATCTTCACAGCTTTACGGCACCAACCTTGTTAATTTATTGAAATTACTTTGTAAAAACAAAGACGGTCAAATTGACATCAATTTtgatg                                                                                        <  1:540232/251‑1 (MQ=255)                                                                                                                                             aaGAAATGGTGGCATCCATGAAACCGGGTAGCGTAATCGTGGATTTGGCAGCAGCCACCGGCGGTAACTGTGAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAACCAAGTGaaaa                                                                                                                                                                                                                         <  1:86810/120‑1 (MQ=255)                                                                                                                                             aaGAAATGGTGGCATCCATGAAACCGGGTAGCGTAATCGTGGATTTGGCAGCAGCCACCGGCGGTAACTGTGAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTGATTTGCCAAGCCGTTTACCAACCCAATCTTCACAGCTTTACGGCACCAACCTTGTTAATTTATTGAAATTACTTTGTAAAAACAAAGAc                                                                                                                 <  1:319289/224‑1 (MQ=255)                                                                                                                                              aGAAATGGTGGCATCCATGAAACCGGGTAGCGTAATCGTGGATTTGGCAGCAGCCACCGGCGGTAACTGTGAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTGATTTGCCAAGCCGTTTACCAACCCAATCTTCACAGCTTTACGGCACCAACCTTGTTAATTTATTGAAATTACTTTGTAAAAACAAAGACGGTCAAATTGACATCAATTTTGatgatg                                                                                     >  1:666282/1‑251 (MQ=255)                                                                                                                                               gAAATGGTGGCATCCATGAAACCGGGTAGCGTAATCGTGGATTTGGCAGCAGCCACCGGCGGTAACTGTGAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTGATTTGCCAAGCCGTTTACCAACCCAATc                                                                                                                                                                              <  1:687217/161‑1 (MQ=255)                                                                                                                                               gAAATGGTGGCATCCATGAAACCGGGTAGCGTAATCGTGGATTTGGCAGCAGCCACCGGCGGTAACTGTGAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTGATTTGCCAAGCCGTTTACCAACCCAATCTTCACAGCTTTACGGCACCAACCTTGTTAATTTATTGAAATTACTTTGTAAAAACAAAGACGGTCAAATTGACATCAATTTTGATGATgt                                                                                    >  1:31244/1‑251 (MQ=255)                                                                                                                                               gAAATGGTGGCATCCATGAAACCGGGTAGCGTAATCGTGGATTTGGCAGCAGCCACCGGCGGTAACTGTGAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTGATTTGCCAAGCCGTTTACCAACCCAATCTTCACAGCTTTACGGCACCAACCTTGTTAATTTATTGAAATTACTTTGTAAAAACAAAGACGGTCAAATTGACATCAATTTTGATGATgt                                                                                    >  1:469905/1‑251 (MQ=255)                                                                                                                                                     gtggCATCCATGAAACCGGGTAGCGTAATCGTGGATTTGGCAGCAGCCACCGGCGGTAACTGTGAACTCAGCAAAGCAGGTGAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTGATTTGCCAAGCCGTTTACCAACCCAATCTTCACAGCTTTACGGCACCAACCTTGTTAATTTATTGAAATTACTTTGTAAAAACAAAGACGGTCAAATTGACATCAATTTTGATGATGTAGTAt                                                                               <  1:446714/250‑1 (MQ=255)                                                                                                                                                     gtggCATCCATGAAACCGGGTAGCGTAATCGTGGATTTGGCAGCAGCCACCGGCGGTAACTGTGAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTGATTTGCCAAGCCGTTTACCAACCCAATCTTCACAGCTTTACGGCACCAACCTTGTTAATTTATTGAAATTACTTTGTAAAAACAAAGACGGTCAAATTGACATCAATTTTGATGATGTAGTAt                                                                               <  1:259664/250‑1 (MQ=255)                                                                                                                                                     gtggCATCCATGAAACCGGGTAGCGTAATCGTGGATTTGGCAGCAGCCACCGGCGGTAACTGTGAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTGATTTGCCAAGCCGTTTACCAACCCAATCTTCACAGCTTTACGGCACCAACCTTGTTAATTTATTGAAATTACTTTGTAAAAACAAAGACGGTCAAATTGACATCAATTTTGATGATGTAGTAt                                                                               <  1:352373/250‑1 (MQ=255)                                                                                                                                                     gtggCATCCATGAAACCGGGTAGCGTAATCGTGGATTTGGCAGCAGCCACCGGCGGTAACTGTGAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTGATTTGCCAAGCCGTTTACCAACCCAATCTTCACAGCTTTACGGCACCAACCTTGTTAATTTATTGAAATTACTTTGTAAAAACAAAGACGGTCAAATTGACATCAATTTTGATGATGTAGTAt                                                                               <  1:392668/250‑1 (MQ=255)                                                                                                                                                     gtggCATCCATGAAACCGGGTAGCGTAATCGTGGATTTGGCAGCAGCCACCGGCGGTAACTGTGAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTGATTTGCCAAGCCGTTTACCAACCCAATCTTCACAGCTTTACGGCACCAACCTTGTTAATTTATTGAAATTACTTTGTAAAAACAAAGACGGTCAAATTGACATCAATTTTGATGATGTAGTAt                                                                               <  1:847459/250‑1 (MQ=255)                                                                                                                                                            ccATGAAACCGGGTAGCGTAATCGTGGATTTGGCAGCAGCCACCGGCGGTAACTGTGAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTGATTTGCCAAGCCGTTTACCAACCCAATCTTCACAGCTTTACGGCACCAACCTTGTTAATTTATTGAAATTACTTTGTAAAAACAAAGACGGTCAAAt                                                                                                         <  1:347814/217‑1 (MQ=255)                                                                                                                                                            ccATGAAACCGGGTAGCGTAATCGTGGATTTGGCAGCAGCCACCGGCGGTAACTGTGAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTGATTTGCCAAGCCGTTTACCAACCCAATCTTCACAGCTTTACGGCACCAACCTTGTTAATTTATTGAAATTACTTTGTAAAAACAAAGACGGTCAAATTGACATCAATTTTGATGATGTAGTAt                                                                               <  1:36999/243‑1 (MQ=255)                                                                                                                                                            ccATGAAACCGGGTAGCGTAATCGTGGATTTGGCAGCAGCCACCGGCGGTAACTGTGAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTGATTTGCCAAGCCGTTTACCAACCCAATCTTCACAGCTTTACGGCACCAACCTTGTTAATTTATTGAAATTACTTTGTAAAAACAAAGACGGTCAAATTGACATCAATTTTGATGATGTAGTATTgcgtgg                                                                        >  1:573026/1‑250 (MQ=255)                                                                                                                                                                 aaaCCGGGTAGCGTAATCGTGGATTTGGCAGCAGCCACCGGCGGTAACTGTGAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTGATTTGCCAAGCCGTTTACCAACCCAATCTTCACAGCTTTACGGCACCAACCTTGTTAATTTATTGAAATTACTTTGTAAAAACAAAGACGGTCAAATTg                                                                                                       <  1:778482/214‑1 (MQ=255)                                                                                                                                                                         tAGCGTAATCGTGGATTTGGCAGCAGCCACCGGCGGTAACTGTGAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTGATTTGCCAAGCCGTTTACCAACCCAATCTTCACAGCTTTACGGCACCAACCTTGTTAATTTATTGAAATTACTTTGTAAAAACAAAGACGGTCAAATTGACATCAATTTTGATGATGTAGTATTGCGTGGCGTGACTGTGAtt                                                           >  1:266412/1‑250 (MQ=255)                                                                                                                                                                           gCGTAATCGTGGATTTGGCAGCAGCCACCGGCGGTAACTGTGAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTGATTTGCCAAGCCGTTTACCAACCCAATCTTCACAGCTTTACGGCACCAACCTTGTTAATTTATTGAAATTACTTTGTAAAAACAAAGACGGTCAAATTGACATCAATTTTGATGATGTAGTATTgcgtgg                                                                        >  1:186024/1‑235 (MQ=255)                                                                                                                                                                           gCGTAATCGTGGATTTGGCAGCAGCCACCGGCGGTAACTGTGAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTGATTTGCCAAGCCGTTTACCAACCCAATCTTCACAGCTTTACGGCACCAACCTTGTTAATTTATTGAAATTACTTTGTAAAAACAAAGACGGTCAAATTGACATCAATTTTGATGATGTAGTATTGCGTGGCGTGACTGTGATTCGt                                                        >  1:314069/1‑251 (MQ=255)                                                                                                                                                                              tAATCGTGGATTTGGCAGCAGCCACCGGCGGTAACTGTGAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTGATTTGCCAAGCCGTTTACCAACCCAATCTTCACAGCTTTACGGCACCAACCTTGTTGATTTATTGAAATTACTTTGTAAAAACAAAGACGGTCAAATTGACATCAATTTTGATGATGTAGTATTGCGTGGCGTGACTGTGATTCGTGa                                                      <  1:660658/250‑1 (MQ=255)                                                                                                                                                                                             cagcagCCACCGGCGGTAACTGTGAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTGATTTGCCAAGCCGTTTACCAACCCAATCTTCACAGCTTTACGGCACCAACCTTGTTAATTTATTGAAATTACTTTGTAAAAACAAAGACGGTCAAATTGACATCAATTTTGATGATGTAGTATTGCGTGGCGTGACTGTGATTCGTGACGGTGAAATTACTTg                                       <  1:281166/250‑1 (MQ=255)                                                                                                                                                                                               gcagcCACCGGCGGTAACTGTGAACTCAGCAAAGCAGGCGAAgtg                                                                                                                                                                                                                                                  <  1:720826/45‑1 (MQ=255)                                                                                                                                                                                                  gcCACCGGCGGTAACTGTGAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTGATTTGCCAAGCCGTTTACCAACCCAATCTTCACAGCTTTACGGCACCAACCTTGTTAATTTATTGAAATTACTTTGTAAAAACAAAGACGGTCAAATTGACATCAATTTTGATGATGTAGTATTGCGTGGCGTGACTGTGATTCGTGACGGTGAAATTACTTGGCCgg                                  >  1:530754/1‑250 (MQ=255)                                                                                                                                                                                                       cggcggTAACTGTGAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTGATTTGCCAAGCCGTTTACCAACCCAATCTTCACAGCTTTACGGCACCAACCTTGTTAATTTATTGAAATTACTTTGTAAAAACAAAGACGGTCAAATTGACATCAATTTTGATGATGTAGTATTg                                                                             <  1:691441/202‑1 (MQ=255)                                                                                                                                                                                                        ggcggTAACTGTGAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTGATTTGCCAAGCCGTTTACCAACCCAATCTTCACAGCTTTACGGCACCAACCTTGTTAATTTATTGAAATTACTTTGTAAAAACAAAGACGGTCAAATTGACATCAATTTTGATGATGTAGTATTgcgtggcgt                                                                     >  1:146526/1‑209 (MQ=255)                                                                                                                                                                                                         gcggTAACTGTGAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTGATTTGCCAAGCCGTTTACCAACCCAATCTTCACAGCTTTACGGCACCAACCTTGTTAATTTATTGAAATTACTTTGTAAAAACAAAGACGGTCAAATTGACATCAATTTTGATGATGTAGTATTgcgt                                                                          <  1:26876/203‑1 (MQ=255)                                                                                                                                                                                                               aCTGTGAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTGATTTGCCAAGCCGTTTACCAACCCAATCTTCACAGCTTTACGGCACCAACCTTGTTAATTTATTGAAATTACTTTGTAAAAACAAAGACGGTCAAATTGACATCAATTTtgatg                                                                                        <  1:889987/183‑1 (MQ=255)                                                                                                                                                                                                               aCTGTGAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTGATTTGCCAAGCCGTTTACCAACCCAATCTTCACAGCTTTACGGCACCAACCTTGTTAATTTATTGAAATTACTTTGTAAAAACAAAGACGGTCAAATTGACATCAATTTTGATGATGTAGTATTGCGTGGCGTGACTGTGa                                                             >  1:290300/1‑210 (MQ=255)                                                                                                                                                                                                               aCTGTGAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTGATTTGCCAAGCCGTTTACCAACCCAATCTTCACAGCTTTACGGCACCAACCTTGTTAATTTATTGAAATTACTTTGTAAAAACAAAGACGGTCAAATTGACATCAATTTTGATGATGTAGTATTGCGTGGCGTGACTGTGATTCGTGACGGTGAAATTACTTGGCCGGCACCACCGATTCAg                    >  1:737170/1‑251 (MQ=255)                                                                                                                                                                                                               aCTGAGAACTCAGCAAAGCAGGCGAAGTGGAGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTGATTTGCCAAGCCGTTTACCAACCCAATCTTCACAGCTTTACGGCACCAACCTTGTTAATTTATTGAAATTACTTTGTAAAAACAAAGACGGTCAAATTGACATCAATTTTGATGATGTAGTATTGCGTGGCGTGACTGTGATTCGTGACGGTGAAATTACTTGGCCGGCACCACCGATTCAg                    >  1:335296/1‑251 (MQ=255)                                                                                                                                                                                                                 tgtgAACTCAGCAAAGCAGGCGAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTGATTTGCCAAGCCGTTTACCAACCCAATCTTCACAGCTTTACGGCACCAACCTTGTTAATTTATTGAAATTACTTTGTAAAAACAAAGACGGTCAAATTGACATCAATTTTGATGATGTAGTAt                                                                               >  1:91330/1‑190 (MQ=255)                                                                                                                                                                                                                         cAGCAAAGCAGGCGAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTGATTTGCCAAGCCGTTTACCAACCCAATCTTCACAGCTTTACGGCACCAACCTTGTTAATTTATTGAAATTACTTTGTAAAAACAAAGACGGTCAAATTGACATCAATTTTGATGATGTAGTAt                                                                               <  1:280552/182‑1 (MQ=255)                                                                                                                                                                                                                         cAGCAAAGCAGGCGAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTGATTTGCCAAGCCGTTTACCAACCCAATCTTCACAGCTTTACGGCACCAACCTTGTTAATTTATTGAAATTACTTTGTAAAAACAAAGACGGTCAAATTGACATCAATTTTGATGATGTAGTATTgcgt                                                                          >  1:650345/1‑187 (MQ=255)                                                                                                                                                                                                                           gCAAAGCAGGCGAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTGATTTGCCAAGCCGTTTACCAACCCAATCTTCACAGCTTTACGGCACCAACCTTGTTAATTTATTGAAATTACTTTGTAAAAACAAAGACGGTCAAATTGACATCAATTTTGATGATGTAGTATTgcgt                                                                          >  1:86477/1‑185 (MQ=255)                                                                                                                                                                                                                                 cAGGCGAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTGATTTGCCAAGCCGTTTACCAACCCAATCTTCACAGCTTTACGGCACCAACCTTGTTAATTTATTGAAATTACTTTGTAAAAACAAAGACGGTCAAATTGACATCAATTTtgatg                                                                                        >  1:582249/1‑165 (MQ=255)                                                                                                                                                                                                                                 cAGGCGAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTGATTTGCCAAGCCGTTTACCAACCCAATCTTCACAGCTTTACGGCACCAACCTTGTTAATTTATTGAAATTACTTTGTAAAAACAAAGACGGTCAAATTGACATCAATTTTGATGATGTAGTATTGCGTGGCGTGACTGTGATTCGTGACGGTGAAATTACTTGGCCGGCACCACCGATTCAGGTTTCCGCTCAGCCgcag  >  1:122149/1‑251 (MQ=255)                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      CGGCGACGGTTATGCGAAAGTGATGTCCGATGAGTTCAATCGTCGCGCGTTAGAACTTTACGCCGAACAAGCGAAAGAAGTGGATATTATTATTACCACCGCGTTAATTCCGGGCAAACCGGCGCCACGCTTGATCACCAAAGAAATGGTGGCATCCATGAAACCGGGTAGCGTAATCGTGGATTTGGCAGCAGCCACCGGCGGTAACTGTGAACTCAGCAAAGCAGGCAAAGTGGTGGTTACCGACAACCAAGTGAAAATCATCGGTTACACTGATTTGCCAAGCCGTTTACCAACCCAATCTTCACAGCTTTACGGCACCAACCTTGTTAATTTATTGAAATTACTTTGTAAAAACAAAGACGGTCAAATTGACATCAATTTTGATGATGTAGTATTGCGTGGCGTGACTGTGATTCGTGACGGTGAAATTACTTGGCCGGCACCACCGATTCAGGTTTCCGCTCAGCCGCAG  >  1/1156729‑1157203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑