Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,155,945	G→T	intergenic (+16815/+237366)	unknown → / ← unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,155,945	0	G	T	100.0%	99.8 / NA	36	intergenic (+16815/+237366)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (21/15);  total (21/15)

GAATTAAGGTTGCGGATTGGAGAGATGGTAATTGCATAGTGTTGACCTTTGAACAATAAAGTGCGGTTGATTTTTTAAGTGTTTTTAGCACACCAAAGTTAAATAGTGGAAAACGATTAGTGCGCTAAAATAGCAAAGTTAGTAGTGAATTTCTAA‑TTTTTTTGCATAAATGAAGTATTGTAATAGGGATTACATATCAAAGAAAAAGCTGAAAAAATTTTTTTACTTTTTCCTCATCAATGATAGAGTTAGTTACAGTTTGGCTGTTTTAGAATAGTCACTTGGGGGGTAATTTTACCCGGATTATTAATCAATCAAAGGGAATCATTATGTTAATTGGTGTACCTAGAGAACTACTTGATAATGAAAGTCGCGTGGCGGCAACGCCGAAGACGGTTCAGCAAATTTTGAAGCTTGGCTTTGAGGTGATTGTTGAACATGATGC  >  1/1155735‑1156179                                                                                                                                                                                                                    |                                                                                                                                                                                                                                           gaATTAAGGTTGCGGATTGGAGAGATGGTAATTGCATAGTGTTGACCTTTGAACAATAAAGTGCGGTTGATTTTTTAAGTGTTTTTAGCACACCAAAGTTAAATAGTGGAAAACGATTAGTGCGCTAAAATAGCAAAGTTAGTAGTGAATTTCTAA‑TTTTTTTGCATAAATGAAGTATTGTAATAGGGATTACATATCAAAGAAAAAGCTTAAAAAATTTTTTTACTTTTTCCTCATCAATGATAGagtta                                                                                                                                                                                                    >  1:880483/1‑251 (MQ=255)  aaTTAAGGTTGCGGATTGGAGAGATGGTAATTGCATAGTGTTGACCTTTGAACAATAAAGTGCGGTTGATTTTTTAAGTGTTTTTAGCACACCAAAGTTAAATAGTGGAAAACGATTAGTGCGCTAAAATAGCAAAGTTAGTAGTGAATTTCTAA‑TTTTTTTGCATAAATGAAGTATTGTAATAGGGATTACATATCAAAGAAAAAGCTTAAAAAATTTTTTTACTTTTTCCTCATCAATGATagag                                                                                                                                                                                                       <  1:763769/247‑1 (MQ=255)      aaGGTTGCGGATTGGAGAGATGGTAATTGCATAGTGTTGACCTTTGAACAATAAAGTGCGGTTGATTTTTTAAGTGTTTTTAGCACACCAAAGTTAAATAGTGGAAAACGATTAGTGCGCTAAAATAGCAAAGTTAGTAGTGAATTTCTAA‑TTTTTTTGCATAAATGAAGTATTGTAATAGGGATTACATATCAAAGAAAAAGCTTAAAAAATTTTTTTACTTTTTCCTCATCAATGATAGAGTTAGTTAc                                                                                                                                                                                               <  1:138613/251‑1 (MQ=255)               gATTGGAGAGATGGTAATTGCATAGTGTTGACCTTTGAACAATAAAGTGCGGTTGATTTTTTAAGTGTTTTTAGCACACCAAAGTTAAATAGTGGAAAACGATTAGTGCGCTAAAATAGCAAAGTTAGTAGTGAATTTCTAA‑TTTTTTTGCATAAATGAAGTATTGTAATAGGGATTACATATCAAAGAAAAAGCTTAAAAAATTTTTTTACTTTTTCCTCATCAATGATAGAGTTAGTTACAGTTTgg                                                                                                                                                                                        >  1:753976/1‑249 (MQ=255)               gATTGGAGAGATGGTAATTGCATAGTGTTGACCTTTGAACAATAAAGTGCGGTTGATTTTTTAAGTGTTTTTAGCACACCAAAGTTAAATAGTGGAAAACGATTAGTGCGCTAAAATAGCAAAGTTAGTAGTGAATTTCTAA‑TTTTTTTGCATAAATGAAGTATTGTAATAGGGATTACATATCAAAGAAAAAGCTTAAAAAATTTTTTTACTTTTTCCTCATCAATGATAGAGTTAGTTACAGTTTgg                                                                                                                                                                                        >  1:667686/1‑249 (MQ=255)                             tAATTGCATAGTGTTGACCTTTGAACAATAAAGTGCGGTTGATTTTTTAAGTGTTTTTAGCACACCAAAGTTAAATAGTGGAAAACGATTAGTGCGCTAAAATAGCAAAGTTAGTAGTGAATTTCTAA‑TTTTTTTGCATAAATGAAGTATTGTAATAGGGATTACATATCAAAGAAAAAGCTTAAAAAATTTTTTTACTTTTTCCTCATCAATGATAGAGTTAGTTACAGTTTGGCTGTTTTAGAATAGTc                                                                                                                                                                        <  1:475159/251‑1 (MQ=255)                                       gtgtTGACCTTTGAACAATAAAGTGCGGTTGATTTTTTAAGTGTTTTTAGCACACCAAAGTTAAATAGTGGAAAACGATTAGTGCGCTAAAATAGCAAAGTTAGTAGTGAATTTCTAA‑TTTTTTTGCATAAATGAAGTATTGTAATAGGGATTACATATCAAAGAAAAAGCTTAAAAAATTTTTTTACTTTTTCCTCATCAATGATAGAGTTAGTTACAGTTTGGCTGTTTTAGAATAGTCACTTgggggg                                                                                                                                                              >  1:156028/1‑251 (MQ=255)                                       gtgtTGACCTTTGAACAATAAAGTGCGGTTGATTTTTTAAGTGTTTTTAGCACACCAAAGTTAAATAGTGGAAAACGATTAGTGCGCTAAAATAGCAAAGTTAGTAGTGAATTTCTAA‑TTTTTTTGCATAAATGAAGTATTGTAATAGGGATTACATATCAAAGAAAAAGCTTAAAAAATTTTTTTACTTTTTCCTCATCAATGATAGAGTTAGTTACAGTTTGGCTGTTTTAGAATAGTCACTTgggggg                                                                                                                                                              >  1:210791/1‑251 (MQ=255)                                                      cAATAAAGTGCGGTTGATTTTTTAAGTGTTTTTAGCACACCAAAGTTAAATAGTGGAAAACGATTAGTGCGCTAAAATAGCAAAGTTAGTAGTAAATTTCTAA‑TTTTTTTGCATAAATGAAGTATTGTAATAGGGATTACATATCAAAGAAAAAGCTTAAAAAATTTTTTTACTTTTTCCTCATCAATGATAGAGTTAGTTACAGTTTGGCTGTTTTAGAATAGTCACtt                                                                                                                                                                    <  1:501152/230‑1 (MQ=255)                                                             gTGCGGTTGATTTTTTAAGTGTTTTTAGCACACCAAAGTTAAATAGTGGAAAACGATTAGTGCGCTAAAATAGCAAAGTTAGTAGTGAATTTCTAA‑TTTTTTTGCATAAATGAAGTATTGTAATAGGGATTACATATCAAAGAAAAAGCTTAAAAAATTTTTTTACTTTTTCCTCATCAATGATAGAGTTAGTTACAGTTTGGCTGTTTTAGAATAGTCACTTGGGGGGTAATTTTACCCGGATTATTaat                                                                                                                                        >  1:507234/1‑251 (MQ=255)                                                                       ttttttAAGTGTTTTTAGCACACCAAAGTTAAATAGTGGAAAACGATTAGTGCGCTAAAATAGCAAAGTTAGTAGTGAATTTCTAA‑TTTTTTTGCATAAATGAAGTATTGTAATAGGGATTACATATCAAAGAAAAAGCTTAAAAAATTTTTTTACTTTTTCCTCATCAATGATAGAGTTAGTTACAGTTTGGCTGTTTTAGAATAGTCACTTGGGGGGTAATTTTACCCGGATTATTAATCAATCAAAgg                                                                                                                              >  1:198066/1‑251 (MQ=255)                                                                                 gtTTTTAGCACACCAAAGTTAAATAGTGGAAAACGATTAGTGCGCTAAAATAGCAAAGTTAGTAGTGAATTTCTAA‑TTTTTTTGCATAAATGAAGTATTGTAATAGGGATTACATATCAAAGAAAAAGCTTAAAAAATTTTTTTACTTTTTCCTCATCAATGATAGagttagtt                                                                                                                                                                                                 <  1:130697/174‑1 (MQ=255)                                                                                           cacCAAAGTTAAATAGTGGAAAACGATTAGTGCGCTAAAATAGCAAAGTTAGTAGTGAATTTCTAA‑TTTTTTTGCATAAATGAAGTATTGTAATAGGGATTACATATCAAAGAAAAAGCTTAAAAAATTTTTTTACTTTTTCCTCATCAATGATAGAGTTAGTTACAGTTTGGCTGTTTTAGAATAGTCACTTGGGGGGTAATTTTACCCGGATTATTAATCAATCAAAGGGAATCATTATGTTAATTgg                                                                                                           >  1:214151/1‑250 (MQ=255)                                                                                           aacCAAAGTTAAATTGTTGAAAACGATTAATTCGCTAAAATAGCAAAGTTAGTAATGAATTTCTAATTTTTTTTGCATAAATGAAGTATTGTAATAGGGATTACATATCAAAGAAAAAGCTTAAAAAATTTTTTTACTTTTTCCTCATCAATGATAGAGTTAGTTACAGTTTGGCTGTTTTAGAATAGTCACTTGGGGGGTAATTTTACCCGGATTATTAATCAATCAAAGGGAATCATTATGTTAATTgg                                                                                                           <  1:285428/250‑1 (MQ=255)                                                                                                aaGTTAAATAGTGGAAAACGATTAGTGCGCTAAAATAGCAAAGTTAGTAGTGAATTTCTAA‑TTTTTTTGCATAAATGAAGTATTGTAATAGGGATTACATATCAAAGAAAAAGCTTAAAAAATTTTTTTACTTTTTCCTCATCAATGATAGAGTTAGTTACAGTTTGGCTGTTTTAGAATAGTCACTTGGGGGGTAATTTTACCCGGATTATTAATCAATCAAAGGGAATCATTATGTTAATTGGTGTAc                                                                                                      >  1:679260/1‑250 (MQ=255)                                                                                                  gTTAAATAGTGGAAAACGATTAGTGCGCTAAAATAGCAAAGTTAGTAGTGAATTTCTAA‑TTTTTTTGCATAAATGAAGTATTGTAATAGGGATTACATATCAAAGAAAAAGCTTAAAAAATTTTTTTACTTTTTCCTCATCAATGATAGAGTTAGTTACAGTTTGGCTGTTTTAGAATAGTCACTTGGGGGGTAATTTTACCCGGATTATTAATCAATCAAAg                                                                                                                               <  1:536341/223‑1 (MQ=255)                                                                                                  gTTAAATAGTGGAAAACGATTAGTGCGCTAAAATAGCAAAGTTAGTAGTGAATTTCTAA‑TTTTTTTGCATAAATGAAGTATTGTAATAGGGATTACATATCAAAGAAAAAGCTTAAAAAATTTTTTTACTTTTTCCTCATCAATGATAGAGTTAGTTACAGTTTGGCTGTTTTAGAATAGTCACTTGGGGGGTAATTTTACCCGGATTATTAATCAATCAAAGGGAATCATTATGTTAAtt                                                                                                             >  1:137100/1‑241 (MQ=255)                                                                                                  gTTAAATAGTGGAAAACGATTAGTGCGCTAAAATAGCAAAGTTAGTAGTGAATTTCTAA‑TTTTTTTGCATAAATGAAGTATTGTAATAGGGATTACATATCAAAGAAAAAGCTTAAAAAATTTTTTTACTTTTTCCTCATCAATGATAGAGTTAGTTACAGTTTGGCTGTTTTAGAATAGTCACTTGGGGGGTAATTTTACCCGGATTATTAATCAATCAAAGGGAATCATTATGTTAATTGGTGTACCt                                                                                                    >  1:376541/1‑250 (MQ=255)                                                                                                                  cGATTAGTGCGCTAAAATAGCAAAGTTAGTAGTGAATTTCTAA‑TTTTTTTGCATAAATGAAGTATTG‑AATAGGGATTACATATCAAAGAAAAAGCTTAAAAAATTTTTTTACTTTTTCCTCATCAATGATAGAGTTAGTTACAGTTTGGCTGTTTTAGAATAGTCACTTgggggg                                                                                                                                                              >  1:189082/1‑175 (MQ=255)                                                                                                                         tGCGCTAAAATAGCAAAGTTAGTAGTGAATTTCTAA‑TTTTTTTGCATAAATGAAGTATTGTAATAGGGATTACATATCAAAGAAAAAGCTTAAAAAATTTTTTTACTTTTTCCTCATCAATGATAGAGTTAGTTACAGTTTGGCTGTTTTAGAATAGTCACTTGGGGGGTAATTTTACCCGGATTATTAATCAATCAAAGGGAATCATTATGTTAATTGGTGTACCTAGAGAACTACTTGATAATGAAAGt                                                                            <  1:394276/251‑1 (MQ=255)                                                                                                                                          gTTAGTAGTGAATTTCTAA‑TTTTTTTGCATAAATGAAGTATTGTAATAGGGATTACATATCAAAGAAAAAGCTTAAAAAATTTTTTTACTTTTTCCTCATCAATGATAGAGTTAGTTACAGTTTGGCTGTTTTAGaa                                                                                                                                                                             <  1:568006/137‑1 (MQ=255)                                                                                                                                                                                gTATTGTAATCGGGATTACATATCAAAGAAAAAGCTTAAAAAATTTTTTTACTTTTTCCTCATCAATGATAGAGTTAGTTACAGTTTGGCTGTTTTAGAATAGTCACTTGGGGGGTAATTTTACCCGGATTATTAATCAATCAAAGGGAATCATTATGTTAATTGGTGTACCTAGAGAACTACTTGATAATGAAAGTCGCGTGGCGGCAACGCCGAAGACGGTTCAGCAAATTTTGAAGCTTGGCTTTGAg                      >  1:714360/1‑251 (MQ=255)                                                                                                                                                                                gTATTGTAATAGGGATTACATATCAAAGAAAAAGCTTAAAAAATTTTTTTACTTTTTCCTCATCAATGATAGAGTTAGTTACAGTTTGGCTGTTTTAGAATAGTCACTTGGGGGGTAATTTTACCCGGATTATTAATCAATCAAAGGGAATCATTATGTTAATTGGTGTACCTAGAGAACTACTTGATAATGAAAGTCGCGTGGCGGCAACGCCGAAGACGGTTCAGCAAATTTTGAAGCTTGGCTTTGAg                      >  1:399584/1‑251 (MQ=255)                                                                                                                                                                                 tATTGTAATAGGGATTACATATCAAAGAAAAAGCTTAAAAAATTTTTTTACTTTTTCCTCATCAATGATAGAGTTAGTTACAGTTTGGCTGTTTTAGAATAGTCACTTGGGGGGTAATTTTACCCGGATTATTAATCAATCAAAGGGAATCATTATGTTAATTGGTGTACCTAGAGAACTACTTGATAATGAAAGTCGCGTGGCGGCAACGCCGAAGACGGTTCAGCAAATTTTGAAGCTTGGCTTTGAgg                     <  1:890597/251‑1 (MQ=255)                                                                                                                                                                                  aTTGTAATAGGGATTACATATCAAAGAAAAAGCTTAAAAAATTTTTTTACTTTTTCCTCATCAATGATAGAGTTAGTTACAGTTTGGCTGTTTTAGAATAGTCACTTGGGGGGTAATTTTACCCGGATTATTAATCAATCAAAGGGAATCATTATGTTAATTGGTGTACCTAGAGAACTACTTGATAATGAAAGTCGCGTGGCGGCAACGCCGAAGACGGTTCAGCAAATTTTGAAGCTTGGCTTTGAGGt                    >  1:166183/1‑251 (MQ=255)                                                                                                                                                                                   ttGTAATAGGGATTACATATCAAAGAAAAAGCTTAAAAAATTTTTTTACTTTTTCCTCATCAATGATAGAGTTAGTTACAGTTTGGCTGTTTTAGAATAGTCACTTGGGGGGTAATTTTACCCGGATTATTAATCAATCAAAGGGAATCATTATGTTAATTGGTGTACCTAGAGAACTACTTGATAATGAAAGTcg                                                                          >  1:544176/1‑196 (MQ=255)                                                                                                                                                                                     gTAATAGGGATTACATATCAAAGAAAAAGCTTAAAAAATTTTTTTACTTTTTCCTCATCAATGATAGagt                                                                                                                                                                                                      >  1:308582/1‑70 (MQ=255)                                                                                                                                                                                     gTAATAGGGATTACATATCAAAGAAAAAGCTTAAAAAATTTTTTTACTTTTTCCTCATCAATGATAGAGTTAGTTACAGTTTGGCTGTTTTAGAATAGTCACTTGGGGGGTAATTTTACCCGGATTATTAATCAATCAAAGGGAATCATTATGt                                                                                                                  <  1:830896/154‑1 (MQ=255)                                                                                                                                                                                     gTAATAGGGATTACATATCAAAGAAAAAGCTTAAAAAATTTTTTTACTTTTTCCTCATCAATGATAGAGTTAGTTACAGTTTGGCTGTTTTAGAATAGTCACTTGGGGGGTAATTTTACCCGGATTATTAATCAATCAAAGGGAATCATTATGt                                                                                                                  <  1:831693/154‑1 (MQ=255)                                                                                                                                                                                     gTAATAGGGATTACATATCAAAGAAAAAGCTTAAAAAATTTTTTTACTTTTTCCTCATCAATGATAGAGTTAGTTACAGTTTGGCTGTTTTAGAATAGTCACTTGGGGGGTAATTTTACCCGGATTATTAATCAATCAAAGGGAATCATTATGTTAATTGGTGTACCt                                                                                                    >  1:602061/1‑168 (MQ=255)                                                                                                                                                                                     gTAATAGGGATTACATATCAAAGAAAAAGCTTAAAAAATTTTTTTACTTTTTCCTCATCAATGATAGAGTTAGTTACAGTTTGGCTGTTTTAGAATAGTCACTTGGGGGGTAATTTTACCCGGATTATTAATCAATCAAAGGGAATCATTATGTTAATTGGTGTACCTAGAGAACTACTTGATAATGAAAGt                                                                            <  1:632885/192‑1 (MQ=255)                                                                                                                                                                                     gTAATAGGGATTACATATCAAAGAAAAAGCTTAAAAAATTTTTTTACTTTTTCCTCATCAATGATAGAGTTAGTTACAGTTTGGCTGTTTTAGAATAGTCACTTGGGGGGTAATTTTACCCGGATTATTAATCAATCAAAGGGAATCATTATGTTAATTGGTGTACCTAGAGAACTACTTGATAATGAAAGTCGCGTGGCGGCAACGCCGAAGACGGTTCAGCAAATTTTGAAGCTTGGCTTTGAGGTGAt                 >  1:297998/1‑251 (MQ=255)                                                                                                                                                                                          aGGGATTACATATCAAAGAAAAAGCTTAAAAAATTTTTTTACTTTTTCCTCATCAATGATAGAGTTAGTTACAGTTTGGCTGTTTTAGAATAGTCACTTGGGGGGTAATTTTACCCGGATTATTAATCAATCAAAGGGAATCATTATGTTAATTGGTGTACCTAGAGAACTACTTGATAATGAAAGTCGCGTGGCGGCAACGCCGAAGACGGTTCAGCAAATTTTGAAGCTTGGCTTTGAGGTGAttgttg            >  1:238296/1‑251 (MQ=255)                                                                                                                                                                                               ttACATATCAAAGAAAAAGCTTAAAAAATTTTTTTACTTTTTCCTCATCAATGATAGAGTTAGTTACAGTTTGGCTGTTTTAGAATAGTCACTTgg                                                                                                                                                                  <  1:847252/96‑1 (MQ=255)                                                                                                                                                                                               ttACATATCAAAGAAAAAGCTTAAAAAATTTTTTTACTTTTTCCTCATCAATGATAGAGTTAGTTACAGTTTGGCTGTTTTAGAATAGTCACTTGGGGGGTAATTTTACCCGGATTATTAATCAATCAAAGGGAATCATTATGTTAATTGGTGTACCTAGAGAACTACTTGATAATGAAAGTCGCGTGGCGGCAACGCCGAAGACGGTTCAGCAAATTTTGAAGCTTGGc                            >  1:803931/1‑230 (MQ=255)                                                                                                                                                                                                    tatCAAAGAAAAAGCTTAAAAAATTTTTTTACTTTTTCCTCATCAATGATAGAGTTAGTTACAGTTTGGCTGTTTTAGAATAGTCACTTGGGGGGTAATTTTACCCGGATTATTAATCAATCAAAGGGAATCATTATGTTAATTGGTGTACCTAGAGAACTACTTGATAATGAAAGTCGCGTGGCGGCAACGCCGAAGACGGTTCAGCAAATTTTGAAGCTTGGCTTTGAGGTGATTGTTGAACATGATGc  <  1:559817/251‑1 (MQ=255)                                                                                                                                                                                                                    |                                                                                                                                                                                                                                           GAATTAAGGTTGCGGATTGGAGAGATGGTAATTGCATAGTGTTGACCTTTGAACAATAAAGTGCGGTTGATTTTTTAAGTGTTTTTAGCACACCAAAGTTAAATAGTGGAAAACGATTAGTGCGCTAAAATAGCAAAGTTAGTAGTGAATTTCTAA‑TTTTTTTGCATAAATGAAGTATTGTAATAGGGATTACATATCAAAGAAAAAGCTGAAAAAATTTTTTTACTTTTTCCTCATCAATGATAGAGTTAGTTACAGTTTGGCTGTTTTAGAATAGTCACTTGGGGGGTAATTTTACCCGGATTATTAATCAATCAAAGGGAATCATTATGTTAATTGGTGTACCTAGAGAACTACTTGATAATGAAAGTCGCGTGGCGGCAACGCCGAAGACGGTTCAGCAAATTTTGAAGCTTGGCTTTGAGGTGATTGTTGAACATGATGC  >  1/1155735‑1156179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑