Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,153,243	G→A	intergenic (+14113/+240068)	unknown → / ← unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,153,243	0	G	A	100.0%	146.4 / NA	52	intergenic (+14113/+240068)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/26);  total (26/26)

GATAAAACCGTGGCTGACAATACAGCAATCTTGTTTTAGTAAGGTTGGCATTAATTTCTTAACGCGCTTTTTTTCTTCACCAATATTTTTTTGTGTTTTGTTTAGTCCCAGTAGCCATAAGATTCTGGAAAGAATAAACCATGAATTAAGCGTTAGTTTTATTTTAGGCATATTTGTTATTTTAAGATCAAACTCTTTTAGATCCTCCAATGTAATTAATTCATTACTTGGGAATAAAGCGTTTGCTGTTTTTTGTGCTCTTATCATAGGAGAAACAAATATTTTGGGAATTGTTAACATCTCTTGATATGCAGTACTTTGTTTGAAGGAATTTATTTCATCTAATGCCAAGCGTTCGGTTTCGTTATATTCGACCAAACGATTTTGAGCAACAGTGGCGTTACATTTACTTCCATCAACTAACGGTGTTACATGTCTGATAAGAACGATTTTAGGCATACTATTTAAAAATGTAGTTAAA  >  1/1152996‑1153476                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                          gATAAAACCGTGGCTGACAATACAGCAATCTTGTTTTAGTAAGGTTGGCATTAATTTCTTAACGCGCTTTTTTTCTTCACCAATATTTTTTTGTGTTTTGTTTAGTCCCAGTAGCCATAAGATTCTGGAAAGAATAAACCATGAATTAAGCGTTAGTTTTATTTTAGGCATATTTGTTATTTTAAGATCAAACTCTTTTAGATCCTCCAATGTAATTAATTCATTACTTGGGAATAAAGCGTTTGCTAttt                                                                                                                                                                                                                                        >  1:632847/1‑251 (MQ=255)                  cAATACAGCAATCTTGTTTTAGTAAGGTTGGCATTAATTTCTTAACGCGCTTTTTTTCTTCACCAATATTTTTTTGTGTTTTGTTTAGTCCCAGTAGCCATAAGATTCTGGAAAGAATAAACCATGAATTAAGCGTTAGTTTTATTTTAGGCATATTTGTTATTTTAAGATCAAACTCTTTTAGATCCTCCAATGTAATTAATTCATTACTTGGGAATAAAGCGTTTGCTATTTTTTGTGCTCTTATCATa                                                                                                                                                                                                                       <  1:901838/251‑1 (MQ=255)                  cAATACAGCAATCTTGTTTTAGTAAGGTTGGCATTAATTTCTTAACGCGCTTTTTTTCTTCACCAATATTTTTTTGTGTTTTGTTTAGTCCCAGTAGCCATAAGATTCTGGAAAGAATAAACCATGAATTAAGCGTTAGTTTTATTTTAGGCATATTTGTTATTTTAAGATCAAACTCTTTTAGATCCTCCAATGTAATTAATTCATTACTTGGGAATAAAGCGTTTGCTATTTTTTGTGCTCTTATCATa                                                                                                                                                                                                                       <  1:468275/251‑1 (MQ=255)                    aTACAGCAATCTTGTTTTAGTAAGGTTGGCATTAATTTCTTAACGCGCTTTTTTTCTTCACCAATATTTTTTTGTGTTTTGTTTAGTCCCAGTAGCCATAAGATTCTGGAAAGAATAAACCATGAATTAAGCGTTAGTTTTATTTTAGGCATATTTGTTATTTTAAGATCAAACTCTTTTAGATCCTCCAATGTAATTAATTCATTACTTGGGAATAAAGCGTTTGCTATTTTTTGTGCTCTTATCATAgg                                                                                                                                                                                                                     >  1:89580/1‑251 (MQ=255)                         gCAATCTTGTTTTAGTAAGGTTGGCATTAATTTCTTAACGCGCTTTTTTTCTTCACCAATATTTTTTTGTGTTGTGTTTAGTCCCAGTAGCCATAAGATTCTGGAAAGAATAAACCATGAATTAAGCGTTAGTTTTATTTTAGGCATATTTGTTATTTTAAGATCAAACTCTTTTAGATCCTCCAATGTAATTAATTCATTACTTGGGAATAAAGCGTTTGCTATTTTTTGTGCTCTTATCAt                                                                                                                                                                                                                        <  1:435696/243‑1 (MQ=255)                          cAATCTTGTTTTAGTAAGGTTGGCATTAATTTCTTAACGCGCTTTTTTTCTTCACCAATATTTTTTTGTGTTTTGTTTAGTCCCAGTAGCCATAAGATTCTGGAAAGAATAAACCATGAATTAAGCGTTAGTTTTATTTTAGGCATATTTGTTATTTTAAGATCAAACTCTTTTAGATCCTCCAATGTAATTAATTCATTACTTGGGAATAAAGCGTTTGCTATTTTTTGTGCTCTTATCATAGgag                                                                                                                                                                                                                   <  1:337162/247‑1 (MQ=255)                              cTTGTTTTAGTAAGGTTGGCATTAATTTCTTAACGCGCTTTTTTTCTTCACCAATATTTTTTTGTGTTTTGTTTAGTCCCAGTAGCCATAAGATTCTGGAAAGAATAAACCATGAATTAAGCGTTAGTTTTATTTTAGGCATATTTGTTATTTTAAGATCAAACTCTTTTAGATCCTCCAATGTAATTAATTCATTACTTGGGAATAAAGCGTTTGCTATTTTTTGTGCTCTTATCATAGGAGAAACAAat                                                                                                                                                                                                           <  1:398268/251‑1 (MQ=255)                                 gTTTTAGTAAGGTTGGCATTAATTTCTTAACGCGCTTTTTTTCTTCACCAATATTTTTTTGTGTTTTGTTTAGTCCCAGTAGCCATAAGATTCTGGAAAGAATAAACCATGAATTAAGCGTTAGTTTTATTTTAGGCATATTTGTTATTTTAAGATCAAACTCTTTTAGATCCTCCAATGTAATTAATTCATTACTTGGGAATAAAGCGTTTGCTATTTTTTGTGCTCTTATCATAGGAGAAACAAATAtt                                                                                                                                                                                                        >  1:368398/1‑251 (MQ=255)                                     tAGTAAGGTTGGCATTAATTTCTTAACGCGCTTTTTTTCTTCACCAATATTTTTTTGTGTTTTGTTTAGTCCCAGTAGCCATAAGATTCTGGAAAGAATAAACCATGAATTAAGCGTTAGTTTTATTTTAGGCATATTTGTTATTTTAAGATCAAACTCTTTTAGATCCTCCAATGTAATTAATTCATTACTTGGGAATAAAGCGTTTGCTATTTTTTGTGCTCTTATCATAGGAGAAACAAATATTTTgg                                                                                                                                                                                                    >  1:343730/1‑251 (MQ=255)                                                                 cgcTTTTTTTCTTCACCAATATTTTTTTGTGTTTTGTTTAGTCCCAGTAGCCATAAGATTCTGGAAAGAATAAACCATGAATTAAGCGTTAGTTTTATTTTAGGCATATTTGTTATTTTAAGATCAAACTCTTTTAGATCCTCCAATGTAATTAATTCATTACTTGGGAATAAAGCGTTTGCTATTTTTTGTGCTCTTATCATAGGAGAAACAAATATTTTGGGAATTGTTAACATCTCTTGATATGCAGt                                                                                                                                                                        <  1:283768/251‑1 (MQ=255)                                                                   cTTTTTTTCTTCACCAATATTTTTTTGTGTTTTGTTTAGTCCCAGTAGCCATAAGATTCTGGAAAGAATAAACCATGAATTAAGCGTTAGTTTTATTTTAGGCATATTTGTTATTTTAAGATCAAACTCTTTTAGATCCTCCAATGTAATTAATTCATTACTTGGGAATAAAGCGTTTGCTATTTTTTGTGCTCTTATCATAGGAGAAACAAATATTTTGGGAATTGTTAACATCTCTTGATATGCAGTAc                                                                                                                                                                      <  1:215393/251‑1 (MQ=255)                                                                                                  ttgtttAGTCCCAGTAGCCATAAGATTCTGGAAAGAATAAACCATGAATTAAGCGTTAGTTTTATTTTAGGCATATTTGTTATTTTAAGATCAAACTCTTTTAGATCCTCCAATGTAATTAATTCATTACTTGGGAATAAAGCGTTTGCTATTTTTTGTGCTCTTATCATAGGAGAAACAAATATTTTGGGAATTGTTAACATCTCTTGATATGCAGTACTTTGTTTGAAGGAATTTATTTCATCTAATGc                                                                                                                                       <  1:16549/251‑1 (MQ=255)                                                                                                    gtttAGTCCCTGTAGCCATAAGATTCTGGAAAGAATAAACCATGAATTAAGCGTTAGTTTTATTTTAGGCATATTTGTTATTTTAAGATCAAACTCTTTTAGATCCTCCAATGTAATTAATTCATTACTTGGGAATAAAGCGTTTGCTATTTTTTGTGCTCTTATCATAGGAGAAACAAATATTTTGGGAATTGTTAACATCTCTTGATATGCAGTACTTTGTTTGAAGGAATTTATTTCATCTAATGCCa                                                                                                                                     >  1:1153/1‑251 (MQ=255)                                                                                                    gtttAGTCCCAGTAGCCATAAGATTCTGGAAAGAATAAACCATGAATTAAGCGTTAGTTTTATTTTAGGCATATTTGTTATTTTAAGATCAAACTCTTTTAGATCCTCCAATGTAATTAATTCATTACTTGGGAATAAAGCGTTTGCTATTTTTTGTGCTCTTATCATAGGAGAAACAAATATTTTGGGAATTGTTAACATCTCTTGATATGCAGTACTTTGTTTGAAGGAatttatt                                                                                                                                                  <  1:341875/238‑1 (MQ=255)                                                                                                    gtttAGTCCCAGTAGCCATAAGATTCTGGAAAGAATAAACCATGAATTAAGCGTTAGTTTTATTTTAGGCATATTTGTTATTTTAAGATCAAACTCTTTTAGATCCTCCAATGTAATTAATTCATTACTTGGGAATAAAGCGTTTGCTATTTTTTGTGCTCTTATCATAGGAGAAACAAATATTTTGGGAATTGTTAACATCTCTTGATATGCAGTACTTTGTTTGAAGGAATTTATTTCATCTAATGCCa                                                                                                                                     >  1:175228/1‑251 (MQ=255)                                                                                                        aGTCCCAGTAGCCATAAGATTCTGGAAAGAATAAACCATGAATTAAGCGTTAGTTTTATTTTAGGCATATTTGTTATTTTAAGATCAAACTCTTTTAGATCCTCCAATGTAATTAATTCATTACTTGGGAATAAAGCGTTTGCTATTTTTTGTGCTCTTATCATAGGAGAAACAAATATTTTggg                                                                                                                                                                                                   <  1:60448/185‑1 (MQ=255)                                                                                                                                                aaTTAAGCGTTAGTTTTATTTTAGGCATATTTGTTATTTTAAGATCAAACTCTTTTAGATCCTCCAATGTAATTAATTCATTACTTGGGAATAAAGCGTTTGCTATTTTTTGTGctc                                                                                                                                                                                                                               <  1:192362/117‑1 (MQ=255)                                                                                                                                                            gTTTTATTTTAGGCATATTTGTTATTTTAAGATCAAACTCTTTTAGATCCTCCAATGTAATTAATTCATTACTTGGGAATAAAGCGTTTGCTATTTTTTGTGCTCTTATc                                                                                                                                                                                                                          >  1:185943/1‑110 (MQ=255)                                                                                                                                                                 attttaGGCATATTTGTTATTTTAAGATCAAACTCTTTTAGATCCTCCAATGTAATTAATTCATTACTTGGGAATAAAGCGTTTGCTATTTTTTGTGCTCTTATCATAGGAGAAACAAATATTTTGGGAATTGTTAACATCTCTTGATATGCAGTACTTTGTTTGAAGGAATTTATTTCATCTAATGCCAAGCGTTCGGTTTCGTTATATTCGACCAAACGATTTTGAGCAACAGTGGCGTTACATTTa                                                                          >  1:576342/1‑249 (MQ=255)                                                                                                                                                                       ggCATATTTGTTATTTTAAGATCAAACTCTTTTAGATCCTCCAATGTAATTAATTCATTACTTGGGAATAAAGCGTTTGCTATTTTTTGTGCTCTTATCATAGGAGAAACAAATATTTTGGGAATTGTTAACATCTCTTGATATGCAGTACTTTGTTTGAAGGAATTTATTTCATCTAATGCCAAGCGTTCGGTTTCGTTATATTCGACCAAACGATTTTGAGCAACAGTGGCGTTACATTTACTTCCAt                                                                   >  1:461688/1‑250 (MQ=255)                                                                                                                                                                                           aTCAAACTCTTTTAGATCCTCCAATGTAATTAATTCATTACTTGGGAATAAAGCGTTTGCTATTTTTTGTGCTCTTATCATAGGAGAAACAAATATTTTGGGAATTGTTAACATCTCTTGATATGCAGTACTTTGTTTGAAGGAATTTATTTCATCTAATGCCAAGCGTTCGGTTTCGTTATATTCGACCAAACGATTTTGAGCAACAGTGGCGTTACATTTACTTCCATCAACTAACGGTGTTACATg                                                <  1:698580/249‑1 (MQ=255)                                                                                                                                                                                           aTCAAACTCTTTTAGATCCTCCAATGTAATTAATTCATTACTTGGGAATAAAGCGTTTGCTATTTTTTGTGCTCTTATCATAGGAGAAACAAATATTTTGGGAATTGTTAACATCTCTTGATATGCAGTACTTTGTTTGAAGGAATTTATTTCATCTAATGCCAAGCGTTCGGTTTCGTTATATTCGACCAAACGATTTTGAGCAACAGTGGCGTTACATTTACTTCCATCAACTAACGGTGTTACATGt                                               >  1:74278/1‑250 (MQ=255)                                                                                                                                                                                                   ctTTTAGATCCTCCAATGTAATTAATTCATTACTTGGGAATAAAGCGTTTGCTATTTTTTGTGCTCTTATTATAGGAGAAACAAATATTTTGGGAATTGTTAACATCTCTTGATATGCAGTACTTTGTTTGAAGGAATTTATTTCATCTAATGCCAAGCGTTCGGTTTCGTTATATTCGACCAAACGATTTTGAGCAACAGTGGCGTTACATTTACTTCCATCAACTAACGGTGTTACAt                                                 <  1:429974/240‑1 (MQ=255)                                                                                                                                                                                                   ctTTTAGATCCTCCAATGTAATTAATTCATTACTTGGGAATAAAGCGTTTGCTATTTTTTGTGCTCTTATCATAgg                                                                                                                                                                                                                     >  1:83935/1‑76 (MQ=255)                                                                                                                                                                                                   ctTTTAGATCCTCCAATGTAATTAATTCATTACTTGGGAATAAAGCGTTTGCTATTTTTTGTGCTCTTATCATAGGAGAAACAAATATTTTGGGAATTGTTAACATCTCTTg                                                                                                                                                                                 <  1:641771/112‑1 (MQ=255)                                                                                                                                                                                                   ctTTTAGATCCTCCAATGTAATTAATTCATTACTTGGGAATAAAGCGTTTGCTATTTTTTGTGCTCTTATCATAGGAGAAACAAATATTTTGGGAATTGTTAACATCTCTTGATATGc                                                                                                                                                                           <  1:42516/118‑1 (MQ=255)                                                                                                                                                                                                   ctTTTAGATCCTCCAATGTAATTAATTCATTACTTGGGAATAAAGCGTTTGCTATTTTTTGTGCTCTTATCATAGGAGAAACAAATATTTTGGGAATTGTTAACATCTCTTGATATGCAGTACTTTGTTTGAAGGAATTTATTTCATCTAATGCCAAGCGTTCGGTTTCGTTATATTCGACCAAACGATTTTGAGCAACAg                                                                                        <  1:683156/201‑1 (MQ=255)                                                                                                                                                                                                   ctTTTAGATCCTCCAATGTAATTAATTCATTACTTGGGAATAAAGCGTTTGCTATTTTTTGTGCTCTTATCATAGGAGAAACAAATATTTTGGGAATTGTTAACATCTCTTGATATGCAGTACTTTGTTTGAAGGAATTTATTTCATCTAATGCCAAGCGTTCGGTTTCGTTATATTCGACCAAACGATTTTGAGCAACAGTGGCGTTACATTTACTTCCATCAACTAACgg                                                         <  1:185088/232‑1 (MQ=255)                                                                                                                                                                                                   ctTTTAGATCCTCCAATGTAATTAATTCATTACTTGGGAATAAAGCGTTTGCTATTTTTTGTGCTCTTATCATAGGAGAAACAAATATTTTGGGAATTGTTAACATCTCTTGATATGCAGTACTTTGTTTGAAGGAATTTATTTCATCTAATGCCAAGCGTTCGGTTTCGTTATATTCGACCAAACGATTTTGAGCAACAGTGGCGTTACATTTACTTCCATCAACTAACGGTGTTACAt                                                 >  1:418418/1‑240 (MQ=255)                                                                                                                                                                                                   ctTTTAGATCCTCCAATGTAATTAATTCATTACTTGGGAATAAAGCGTTTGCTATTTTTTGTGCTCTTATCATAGGAGAAACAAATATTTTGGGAATTGTTAACATCTCTTGATATGCAGTACTTTGTTTGAAGGAATTTATTTCATCTAATGCCAAGCGTTCGGTTTCGTTATATTCGACCAAACGATTTTGAGCAACAGTGGCGTTACATTTACTTCCATCAACTAACGGTGTTACATGTCTGATAAGa                                      >  1:137215/1‑251 (MQ=255)                                                                                                                                                                                                   ctTTTAGATCCTCCAATGTAATTAATTCATTACTTGGGAATAAAGCGTTTGCTATTTTTTGTGCTCTTATCATAGGAGAAACAAATATTTTGGGAATTGTTAACATCTCTTGATATGCAGTACTTTGTTTGAAGGAATTTATTTCATCTAATGCCAAGCGTTCGGTTTCGTTATATTCGACCAAACGATTTTGAGCAACAGTGGCGTTACATTTACTTCCATCAACTAACGGTGTTACATGTCTGATAAGa                                      >  1:846149/1‑251 (MQ=255)                                                                                                                                                                                                   ctTTTAGATCCTCCAATGTAATTAATTCATTACTTGGGAATAAAGCGTTTGCTATTTTTTGTGCTCTTATCATAGGAGAAACAAATATTTTGGGAATTGTTAACATCTCTTGATATGCAGTACTTTGTTTGAAGGAATTTATTTCATCTAATGCCAAGCGTTCGGTTTCGTTATATTCGACCAAACGATTTTGAGCAACAGTGGCGTTACATTTACTTCCATCAACTAACGGTGTTACATGTCTGATAAGa                                      >  1:731119/1‑251 (MQ=255)                                                                                                                                                                                                   ctTTTAGATCCTCCAATGTAATTAATTCATTACTTGGGAATAAAGCGTTTGCTATTTTTTGTGCTCTTATCATAGGAGAAACAAATATTTTGGGAATTGTTAACATCTCTTGATATGCAGTACTTTGTTTGAAGGAATTTATTTCATCTAATGCCAAGCGTTCGGTTTCGTTATATTCGACCAAACGATTTTGAGCAACAGTGGCGTTACATTTACTTCCATCAACTAACGGTGTTACATGTCTGATAAGa                                      >  1:302852/1‑251 (MQ=255)                                                                                                                                                                                                   ctTTTAGATCCTCCAATGTAATTAATTCATTACTTGGGAATAAAGCGTTTGCTATTTTTTGTGCTCTTATCATAGGAGAAACAAATATTTTGGGAATTGTTAACATCTCTTGATATGCAGTACTTTGTTTGAAGGAATTTATTTCATCTAATGCCAAGCGTTCGGTTTCGTTATATTCGACCAAACGATTTTGAGCAACAGTGGCGTTACATTTACTTCCATCAACTAACGGTGTTACATGTCTGATAAGa                                      >  1:269161/1‑251 (MQ=255)                                                                                                                                                                                                        aGATCCTCCAATGTAATTAATTCATTACTTGGGAATAAAGCGTTTGCTATTTTTTGTGCTCTTATCATAGGAGAAACAAATATTTTGGGAATTGTTAACATCTCTTGATATGCAGTACTTTGTTTGAAGGAATTTATTTCATCTAATGCCAAGCGTTCGGTTTCGTTATATTCGACCAAACGATTTTGAGCAACAGTGGCGTTACATTTACTTc                                                                      >  1:324451/1‑214 (MQ=255)                                                                                                                                                                                                            cctccAATGTAATTAATTCATTACTTGGGAATAAAGCGTTTGCTATTTTTTGTGCTCTTATCATAGGAGAAACAAATATTTTGGGAATTGTTAACATCTCTTGATATGCAGTACTTTGTTTGAAGGAATTTATTTCATCTAATGCCAAGCGTTCGGTTTCGTTATATTCGACCAAACGATTTTGAGCAACAGTGGCGTTACATTTACTTCCATCAACTAACGGTGTTACATGt                                               >  1:230434/1‑233 (MQ=255)                                                                                                                                                                                                             ctccAATGTAATTAATTCATTACTTGGGAATAAAGCGTTTGCTATTTTTTGTGCTCTTATCATAGGAGAAACAAATATTTTGGGAATTGtt                                                                                                                                                                                            >  1:816568/1‑91 (MQ=255)                                                                                                                                                                                                             ctccAATGTAATTAATTCATTACTTGGGAATAAAGCGTTTGCTATTTTTTGTGCTCTTATCATAGGAGAAACAAATATTTTGGGAATTGTTAACATCTCTTGATATGCAGTACTTTGTTTGAAGGAATTTATTTCATCTAATGCCAAGCGTTCGGTTTCGtt                                                                                                                     <  1:608858/162‑1 (MQ=255)                                                                                                                                                                                                             ctccAATGTAATTAATTCATTACTTGGGAATAAAGCGTTTGCTATTTTTTGTGCTCTTATCATAGGAGAAACAAATATTTTGGGAATTGTTAACATCTCTTGATATGCAGTACTTTGTTTGAAGGAATTTATTTCATCTAATGCCAAGCGTTCGGTTTCGTTATATTCGACCAAACGATTTTGAGCAACAGTGGCGTTACATTTACtt                                                                       <  1:805258/208‑1 (MQ=255)                                                                                                                                                                                                             ctccAATGTAATTAATTCATTACTTGGGAATAAAGCGTTTGCTATTTTTTGTGCTCTTATCATAGGAGAAACAAATATTTTGGGAATTGTTAACATCTCTTGATATGCAGTACTTTGTTTGAAGGAATTTATTTCATCTAATGCCAAGCGTTCGGTTTCGTTATATTCGACCAAACGATTTTGAGCAACAGTGGCGTTACATTTACTTCCATCAACTAACGGTGTTACAt                                                 >  1:458229/1‑230 (MQ=255)                                                                                                                                                                                                             ctccAATGTAATTAATTCATTACTTGGGAATAAAGCGTTTGCTATTTTTTGTGCTCTTATCATAGGAGAAACAAATATTTTGGGAATTGTTAACATCTCTTGATATGCAGTACTTTGTTTGAAGGAATTTATTTCATCTAATGCCAAGCGTTCGGTTTCGTTATATTCGACCAAACGATTTTGAGCAACAGTGGCGTTACATTTACTTCCATCAACTAACGGTGTTACATGTCTGATAAGAACGATTTTAg                            >  1:813388/1‑251 (MQ=255)                                                                                                                                                                                                               ccAATGTAATTAATTCATTACTTGGGAATAAAGCGTTTGCTATTTTTTGTGCTCTTATCATAGGAGAAACAAATATTTTGGGAATTGTTAACATCTCTTGATATGCAGTACTTTGTTTGAAGGAATTTACTTCATCTAATGCCAAGCGTTCGGTTTCGTTATATTCGACCAAACGATTTTGAGCAACAGTGGCGTTACATTTACTTCCATCAACTAACGGTGTTACATGTCTGATAAGAACGATTTTAGGc                          <  1:686408/251‑1 (MQ=255)                                                                                                                                                                                                                    gTAATTAATTCATTACTTGGGAATAAAGCGTTTGCTATTTTTTGTGCTCTTATCATAGGAGAAACAAATATTTTGGGAATTGTTAACATCTCTTGATATGCAGTACTTTGTTTGAAGGAATTTATTTCATCTAATGCCAAGCGTTCGGTTTCGTTATATTCGAc                                                                                                            >  1:41479/1‑164 (MQ=255)                                                                                                                                                                                                                      aattaattCATTACTTGGGAATAAAGCGTTTGCTATTTTTTGTGCTCTTATCATAGGAGAAACAAATATTTTGGGAATTGTTAACATCTCTTGATATGCAGt                                                                                                                                                                        <  1:393276/102‑1 (MQ=255)                                                                                                                                                                                                                           attCATTACTTGGGAATAAAGCGTTTGCTATTTTTTGTGCTCTTATCATAGGAGAAACAAATATTTTGGGAATTGTTAACATCTCTTGATATGCAGTACTTTGTTTGAAGGAATTTATTTCATCTAATGCCAAGCGTTCGGTTTCGTTATATTCGACCAAACGATTTTGAGCAACAGTGGCGTTACATTTACTTCCATCAACTAACGGTGTTACa                                                  <  1:823707/215‑1 (MQ=255)                                                                                                                                                                                                                           attCATTACTTGGGAATAAAGCGTTTGCTATTTTTTGTGCTCTTATCATAGGAGAAACAAATATTTTGGGAATTGTTAACATCTCTTGATATGCAGTACTTTGTTTGAAGGAATTTATTTCATCTAATGCCAAGCGTTCGGTTTCGTTATATTCGACCAAACGATTTTGAGCAACAGTGGCGTTACATTTACTTCCATCAACTAACGGTGTTACATGTCTGATAAGAACGATTTTAGGCATACTATTTaaa              <  1:610308/251‑1 (MQ=255)                                                                                                                                                                                                                                ttACTTGGGAATAAAGCGTTTGCTATTTTTTGTGCTCTTATCATAGGAGAAACAAATATTTTGGGAATTGTTAACATCTCTTGATATGCAGTACTTTGTTTGAAGGAATTTATTTCATCTAATGCCAAGCGTTCGGTTTCGTTATATTCGACCAAACGATTTTGAGCAACAGTGGCGTTACATTTACTTCCATCAACTAACGGTGTTACATGTCTGATAAGAACGATTTTAGGc                          <  1:512971/234‑1 (MQ=255)                                                                                                                                                                                                                                     tGGGAATAAAGCGTTTGCTATTTTTTGTGCTCTTATCATAGGAGAAACAAATATTTTGGGAATTGTTAACAtctc                                                                                                                                                                                    <  1:414389/75‑1 (MQ=255)                                                                                                                                                                                                                                       ggAATAAAGCGTTTGCTATTTTTTGTGCTCTTATCATAGGAGAAACAAATATTTTGGGAATTGTTAACATCTCTTGATATGCAGTACtttgttt                                                                                                                                                               >  1:642193/1‑94 (MQ=255)                                                                                                                                                                                                                                       ggAATAAAGCGTTTGCTATTTTTTGTGCTCTTATCATAGGAGAAACAAATATTTTGGGAATTGTTAACATCTCTTGATATGCAGTACTTTGTTTGAAGGAATTTATTTCATCTAATGCCAAGCGTTCGGTTTCGTTatat                                                                                                                 >  1:534990/1‑140 (MQ=255)                                                                                                                                                                                                                                       ggAATAAAGCGTTTGCTATTTTTTGTGCTCTTATCATAGGAGAAACAAATATTTTGGGAATTGTTAACATCTCTTGATATGCAGTACTTTGTTTGAAGGAATTTATTTCATCTAATGCCAAGCGTTCGGTTTCGTTATATTCGACCAAACGATTTTGAGCAACAGTGGCGTTACATTTACTTCCATCAACTAACGGTGTTACATGTCTGATAAGAACGATTTTAGGCATACTATTTAAAAATGTAGTTaaa  >  1:164509/1‑251 (MQ=255)                                                                                                                                                                                                                                               gCGTTTGCTATTTTTTGTGCTCTTATCATAGGAGAAACAAATATTTTGGGAATTGTTAACAGCTCTTGATATGCAGTACTTTGTTTGAAGGAATTTATTTCATCTAATGCCAAGCGTTCGGTTTCGTTATATTCGACCAAACGATTTTGAGCAACAGTGGCGTTACATTTACTTCCATCAACTAACGGTGTTACATGTCTGATAAGAACGATTTTAGGCATACTATTTaaa              <  1:435608/231‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                          GATAAAACCGTGGCTGACAATACAGCAATCTTGTTTTAGTAAGGTTGGCATTAATTTCTTAACGCGCTTTTTTTCTTCACCAATATTTTTTTGTGTTTTGTTTAGTCCCAGTAGCCATAAGATTCTGGAAAGAATAAACCATGAATTAAGCGTTAGTTTTATTTTAGGCATATTTGTTATTTTAAGATCAAACTCTTTTAGATCCTCCAATGTAATTAATTCATTACTTGGGAATAAAGCGTTTGCTGTTTTTTGTGCTCTTATCATAGGAGAAACAAATATTTTGGGAATTGTTAACATCTCTTGATATGCAGTACTTTGTTTGAAGGAATTTATTTCATCTAATGCCAAGCGTTCGGTTTCGTTATATTCGACCAAACGATTTTGAGCAACAGTGGCGTTACATTTACTTCCATCAACTAACGGTGTTACATGTCTGATAAGAACGATTTTAGGCATACTATTTAAAAATGTAGTTAAA  >  1/1152996‑1153476

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑