Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,145,287	G→A	intergenic (+6157/+248024)	unknown → / ← unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,145,287	0	G	A	100.0%	47.4 / NA	18	intergenic (+6157/+248024)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/4);  total (14/4)

ACTTTATTGATATTTTTTCTTGGCAAAAGACAACCACACCACCTAATTTATCTAAACAAACAAAACAATCTTGCGAATACATTTTAGCTTATCAAAGTGAGAATAGAATATCTCTTACTGGTATTAGAAAAAACAGTAAGAGTTCAAATGGTTTAATGAATCAGACAAACAATATAGGGGTTCTTAAATTTCCTTCAGATATTATTACTACAAAATTACCAGATGGGATTTATAAGGCTGGTATGTGCGGCACATCAAGTTATAAAATTGAATTATTAGAAGATACCGAAGTTAAAAATAATAAGTTTATTAAACCTATTGTGTTAAAAGGAAAATTTAAATGGAGCCAAAAATATTTGGATGATTCAATCAAGAGTGGAGTAAAGGTTATTATTCAAAC  >  1/1145041‑1145440                                                                                                                                                                                                                                                       |                                                                                                                                                          aCTTTATTGATATTTTTTCTTGGCAAAAGACAACCACACCACCTAATTTATCTAAACAAACAAAACAATCTTGCGAATACATTTTAGCTTATCAAAGTGAGAATAGAATATCTCTTACTGGTATTAGAAAAAACAGTAAGAGTTCAAATGGTTTAATGAATCAGACAAACAATATAGGGGTTCTTACATTTCCTTCAGATATTATTACTACAAAATTACCAGATGGGATTTATAAGGCTGGTATGTACgg                                                                                                                                                        >  1:213946/1‑250 (MQ=255)                                            tAATTTATCTAAACAAACAAAACAATCTTGCGAATACATTTTAGCTTATCAAAGTGAGAATAGAATATCTCTTACTGGTATTAGAAAAAACAGTAAGAGTTCAAATGGTTTAATGAATCAGACAAACAATATAGGGGTTCTTACATTTCCTTCAGATATTATTACTACAAAATTACCAGATGGGATTTATAAGGCTGGTATGTACGGCACATCAAGTTATAAAATTGAATTATTAGAAGATACCGAAGTTa                                                                                                            >  1:33059/1‑251 (MQ=255)                                                 tATCTAAACAAACAAAACAATCTTGCGAATACATTTTAGCTTATCAAAGTGAGAATAGAATATCTCTTACTGGTATTAGAAAAAACAGTAAGAGTTCAAATGGTTTAATGAATCAGACAAACAATATAGGGGTTCTTACATTTCCTTCAGATATTATTACTACAAAATTACCAGATGGGATTTATAAGGCTGGTATGTACGGc                                                                                                                                                       >  1:83758/1‑203 (MQ=255)                                                         caaacaaaACAATCTTGCGAATACATTTTAGCTTATCAAAGTGAGAATAGAATATCTCTTACTGGTATTAGAAAAAACAGTAAGAGTTCAAATGGTTTAATGAATCAGACAAACAATATAGGGGTTCTTACATTTCCTTCAGATATTATTACTACAAAATTACCAGATGGGATTTATAAGGCTGGTATGTACGGcac                                                                                                                                                     <  1:245928/197‑1 (MQ=255)                                                                  caaTCTTGCGAATACATTTTAGCTTATCAAAGTGAGAATAGAATATCTCTTACTGGTATTAGAAAAAACAGTAAGAGTTCAAATGGTTTAATGAATCAGACAAACAATATAGGGGTTCTTACATTTCCTTCAGATATTATTACTACAAAATTACCAGATGGGATTTATAAGGCTGGTATGTACGGCACATCAAGTTATAAAATTGAATTATTAGAAGATACCGAAGTTAAAAATAATAAGTTTATTAAAcc                                                                                      <  1:491940/251‑1 (MQ=255)                                                                                                aGTGAGAATAGAATATCTCTTACTGGTATTAGAAAAAACAGTAAGAGTTCAAATGGTTTAATGAATCAGACAAACAATATAGGGGTTCTTACATTTCCTTCAGATATTATTACTACAAAATTACCAGATGGGATTTATAAGGCTGGTATGTACGGCACATCAAGTTATAAAATTGAATTATTAGAAGATACCGAAGTTAAAAATAATAAGTTTATTAAACCTATTGTGTTAAAAGGAAAATTTAAATGGAg                                                        >  1:742613/1‑251 (MQ=255)                                                                                                          gaataTCTCTTACTGGTATTAGAAAAAACAGTAAGAGTTCAAATGGTTTAATGAATCAGACAAACAATATAGGGGTTCTTACATTTCCTTCAGATATTATTACTACAAAATTACCAGATGGGATTTATAAGGCTGGTATGTACgg                                                                                                                                                        >  1:625393/1‑145 (MQ=255)                                                                                                                ctctTACTGGTATTAGAAAAAACAGTAAGAGTTCAAATGGTTTAATGAATCAGACAAACAATATAGGGGTTCTTACATTTCCTTCAGATATTATTACTACAAAATTACCAGATGGGATTTATAAGGCTGGTATGTACGGCACATCAAGTTATAAAATTGAATTATTagaaga                                                                                                                       >  1:859345/1‑172 (MQ=255)                                                                                                                          tATTAGAAAAAACAGTAAGAGTTCAAATGGTTTAATGAATCAGACAAACAATATAGGGGTTCTTACATTTCCTTCAGATATGATTACTACAAAATTACCAGATGGGATTTATAAGGCTGGTATGTACGGCACATCAAGTTATAAAATTGAATTATTAGAAGATACCGAAGTTAAAAATAATAAGTTTATTAAACCTATTGTGTTAAAAGGAAAATTTAAATGGAGCCAAAAATATTTGGATGATtcaatca                              <  1:442962/251‑1 (MQ=255)                                                                                                                                            gagTTCAAATGGTTTAATGAATCAGACAAACAATATAGGGGTTCTTACATTTCCTTCAGATATTATTACTACAAAATTACCAGATGGGATTTATAAGGCTGGTATGTACGGCACATCAAGTTATAAAATTGAATTATTAGAAGATACCGAAGTTAAAAATAATAAGTTTATTAAACCTATTGTGTTAAAAGGAAAATTTAAATGGAGCCAAAAATATTTGGATGATTCAATCAAGAGTGGAGTAAAgg               >  1:749614/1‑248 (MQ=255)                                                                                                                                                       gTTTAATGAATCAGACAAACAATATAGGGGTTCTTACATTTCCTTCAGATATTATTACTACAAAATTACCAGATGGGATTTATAAGGCTGGTATGTACGGCACATCAAGCTATAAAATTGAATTATTAGAAGATACCGAAGTTAAAAATAATAAGTTTATTAAACCTATTGTGTTAAAAGGAAAATTTAAATGGAGCCAAAAATATTTGGATGATTCAATCAAGAGTGGAGTAAAGGTTATTATTCAAAc  >  1:796758/1‑250 (MQ=255)                                                                                                                                                                          caaTATAGGGGTTCTTACATTTCCTTCAGATATTATTACTACAAAATTACCAGATGGGATTTATAAGGCTGGTATGTACGGCACATCAAGTTATAAAATTGAat                                                                                                                                 >  1:69099/1‑104 (MQ=255)                                                                                                                                                                          caaTATAGGGGTTCTTACATTTCCTTCAGATATTATTACTACAAAATTACCAGATGGGATTTATAAGGCTGGTATGTACGGCACATCAAGTTATAAAATTGAATTATTAGAAGATACCGAAGTTAAAAATAATAAGTTTATTAAACCTATTGTGTTaaaa                                                                         >  1:266782/1‑160 (MQ=255)                                                                                                                                                                            atatAGGGGTTCTTACATTTCCTTCAGATATTATTACTACAAAATTACCAGATGGGATTTATAAGGCTGGTATGTACGGCACATCAAGTTATAAAATTGAATTATTAGAAGATACCGAAGt                                                                                                              >  1:274843/1‑121 (MQ=255)                                                                                                                                                                                         tacaTTTCCTTCAGATATTATTACTACAAAATTACCAGATGGGATTTATAAGGCTGGTATGTACGGCACATCAAGTTATAAAATTGAATTATTAGAAGATACCGAAGTTAAAAATAATAAGTTTATTAAACCTATTGTGTTAAAAGGAAAATTTa                                                               >  1:753140/4‑155 (MQ=255)                                                                                                                                                                                                          ttattaCTACAAAATTACCAGATGGGATTTATAAGGCTGGTATGTACGGCACATCAAGTTATAAAATTGAATTATTAGAAGATACCGAAGTTAAAAATAATAAGTTTATTAAACCTATTGTGTTAAAAg                                                                        <  1:235861/129‑1 (MQ=255)                                                                                                                                                                                                                                             gCTGGTATGTACGGCACATCAAGTTATAAAATTGAATTATTAGAAGATACCGAAGTTAAAAATAATAAGTTTATTaa                                                                                         >  1:817327/1‑77 (MQ=255)                                                                                                                                                                                                                                                ggTATGTACGGCACATCAAGTTATAAAATTGAATTATTAGAAGATACCGAAGTTAAAAATAATAAGTTTATTAAACCTATTGTGTTAAAAGGAAAATTTAAATGGAGCCAAAAATATTTGGATGATTCAATCAAGAGTGGAGTAAAGGTTattatt       >  1:717418/1‑156 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                          ACTTTATTGATATTTTTTCTTGGCAAAAGACAACCACACCACCTAATTTATCTAAACAAACAAAACAATCTTGCGAATACATTTTAGCTTATCAAAGTGAGAATAGAATATCTCTTACTGGTATTAGAAAAAACAGTAAGAGTTCAAATGGTTTAATGAATCAGACAAACAATATAGGGGTTCTTAAATTTCCTTCAGATATTATTACTACAAAATTACCAGATGGGATTTATAAGGCTGGTATGTGCGGCACATCAAGTTATAAAATTGAATTATTAGAAGATACCGAAGTTAAAAATAATAAGTTTATTAAACCTATTGTGTTAAAAGGAAAATTTAAATGGAGCCAAAAATATTTGGATGATTCAATCAAGAGTGGAGTAAAGGTTATTATTCAAAC  >  1/1145041‑1145440

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑