Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,145,227	A→C	intergenic (+6097/+248084)	unknown → / ← unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,145,227	0	A	C	100.0%	60.0 / NA	21	intergenic (+6097/+248084)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (14/7);  total (14/7)

GATTATCAATCTGAAAAATATTTTAGATTATACTCTAGGTAAAGATAACTTTATTGATATTTTTTCTTGGCAAAAGACAACCACACCACCTAATTTATCTAAACAAACAAAACAATCTTGCGAATACATTTTAGCTTATCAAAGTGAGAATAGAATATCTCTTACTGGTATTAGAAAAAACAGTAAGAGTTCAAATGGTTTAATGAATCAGACAAACAATATAGGGGTTCTTAAATTTCCTTCAGATATTATTACTACAAAATTACCAGATGGGATTTATAAGGCTGGTATGTGCGGCACATCAAGTTATAAAATTGAATTATTAGAAGATACCGAAGTTAAAAATAATAAGTTTATTAAACCTATTGTGTTAAAAGGAAAATTTAAATGGAGCCAAAAATATTTGGATGATTCAATCAAGAGTGGAGTAAAGGTTATTATTCAAAC  >  1/1144994‑1145440                                                                                                                                                                                                                                          |                                                                                                                                                                                                                      gaTTATCAATCTGAAAAATATTTTAGATTATACTCTAGGTAAAGATAACTTTATTGATATTTTTTCTTGGCAAAAGACAACCACACCACCTAATTTATCTAAACAAACAAAACAATCTTGCGAATACATTTTAGCTTATCAAAGTGAGAATAGAATATCTCTTACTGGTATTAGAAAAAACAGTAAGAGTTCAAATGGTTTAATGAATCAGACAAACAATATAGGGGTTCTTACATTTCCTTCAGAtatta                                                                                                                                                                                                      >  1:70349/1‑251 (MQ=255)       cAATCTGAAAAATATTTTAGATTATACTCTAGGTAAAGATAACTTTATTGATATTTTTTCTTGGCAAAAGACAGCCACACCACCTAATTTATCTAAACAAACAAAACAATCTTGCGAATACATTTTAGCTTATCAAAGTGAGAATAGAATATCTCTTACTGGTATTAGAAAAAACAGTAAGAGTTCAAATGGTTTAATGAATCAGACAAACAATATAGGGGTTCTTACATTTCCTTCAGATATTATtacta                                                                                                                                                                                                <  1:648855/251‑1 (MQ=255)               cagatatTTTAGATTATACTCTAGGTAAAGATAACTTTATTGATATTTTTTCTTGGCAAAAGACAACCACACCACCTAATTTATCTAAACAAACAAAACAATCTTGCGAATACATTTTAGCTTATCAAAGTGAGAATAGAATATCTCTTACTGGTATTAGAAAAAACAGTAAGAGTTCAAATGGTTTAATGAATCAGACAAACAATATAGGGGTTCTTACATTTCCTTCAGATATTATtactac                                                                                                                                                                                               >  1:127101/4‑244 (MQ=255)                                      ggTAAAGATAACTTTATTGATATTTTTTCTTGGCAAAAGACAACCACACCACCTAATTTATCTAAACAAACAAAACAATCTTGCGAATACATTTTAGCTTATCAAAGTGAGAATAGAATATCTCTTACTGGTATTAGAAAAAACAGTAAGAGTTCAAATGGTTTAATGAATCAGACAAACAATATAGGGGTTCTTACATTTCCTTCAGATATTATTACTACAAAATTACCAGATGGGATTTATAAGGCTgg                                                                                                                                                                 >  1:805012/1‑251 (MQ=255)                                             aTAACTTTATTGATATTTTTTCTTGGCAAAAGACAACCACACCACCTAATTTATCTAAACAAACAAAACAATCTTGCGAATACATTTTAGCTTATCAAAGTGAGAATAGAATATCTCTTACTGGTATTAGAAAAAACAGTAAGAGTTCAAATGGTTTAATGAATCAGACAAACAATATAGGGGTTCTTACATTTCCTTCAGATATTATTACTACAAAATTACCAGATGGGATTTATAAGGCTGGTAtgtac                                                                                                                                                          <  1:691671/251‑3 (MQ=255)                                             aTAACTTTATTGATATTTTTTCTTGGCAAAAGACAACCACACCACCTAATTTATCTAAACAAACAAAACAATCTTGCGAATACATTTTAGCTTATCAAAGTGAGAATAGAATATCTCTTACTGGTATTAGAAAAAACAGTAAGAGTTCAAATGGTTTAATGAATCAGACAAACAATATAGGGGTTCTTACATTTCCTTCAGATATTATTACTACAAAATTACCAGATGGGATTTATAAGGCTGGTAtgtac                                                                                                                                                          <  1:398852/251‑3 (MQ=255)                                                aCTTTATTGATATTTTTTCTTGGCAAAAGACAACCACACCACCTAATTTATCTAAACAAACAAAACAATCTTGCGAATACATTTTAGCTTATCAAAGTGAGAATAGAATATCTCTTACTGGTATTAGAAAAAACAGTAAGAGTTCAAATGGTTTAATGAATCAGACAAACAATATAGGGGTTCTTACATTTCCTTCAGATATTATTACTACAAAATTACCAGATGGGATTTATAAGGCTGGTATGTACgg                                                                                                                                                        >  1:213946/1‑250 (MQ=255)                                                                                           tAATTTATCTAAACAAACAAAACAATCTTGCGAATACATTTTAGCTTATCAAAGTGAGAATAGAATATCTCTTACTGGTATTAGAAAAAACAGTAAGAGTTCAAATGGTTTAATGAATCAGACAAACAATATAGGGGTTCTTACATTTCCTTCAGATATTATTACTACAAAATTACCAGATGGGATTTATAAGGCTGGTATGTACGGCACATCAAGTTATAAAATTGAATTATTAGAAGATACCGAAGTTa                                                                                                            >  1:33059/1‑251 (MQ=255)                                                                                                tATCTAAACAAACAAAACAATCTTGCGAATACATTTTAGCTTATCAAAGTGAGAATAGAATATCTCTTACTGGTATTAGAAAAAACAGTAAGAGTTCAAATGGTTTAATGAATCAGACAAACAATATAGGGGTTCTTACATTTCCTTCAGATATTATTACTACAAAATTACCAGATGGGATTTATAAGGCTGGTATGTACGGc                                                                                                                                                       >  1:83758/1‑203 (MQ=255)                                                                                                        caaacaaaACAATCTTGCGAATACATTTTAGCTTATCAAAGTGAGAATAGAATATCTCTTACTGGTATTAGAAAAAACAGTAAGAGTTCAAATGGTTTAATGAATCAGACAAACAATATAGGGGTTCTTACATTTCCTTCAGATATTATTACTACAAAATTACCAGATGGGATTTATAAGGCTGGTATGTACGGcac                                                                                                                                                     <  1:245928/197‑1 (MQ=255)                                                                                                                 caaTCTTGCGAATACATTTTAGCTTATCAAAGTGAGAATAGAATATCTCTTACTGGTATTAGAAAAAACAGTAAGAGTTCAAATGGTTTAATGAATCAGACAAACAATATAGGGGTTCTTACATTTCCTTCAGATATTATTACTACAAAATTACCAGATGGGATTTATAAGGCTGGTATGTACGGCACATCAAGTTATAAAATTGAATTATTAGAAGATACCGAAGTTAAAAATAATAAGTTTATTAAAcc                                                                                      <  1:491940/251‑1 (MQ=255)                                                                                                                                               aGTGAGAATAGAATATCTCTTACTGGTATTAGAAAAAACAGTAAGAGTTCAAATGGTTTAATGAATCAGACAAACAATATAGGGGTTCTTACATTTCCTTCAGATATTATTACTACAAAATTACCAGATGGGATTTATAAGGCTGGTATGTACGGCACATCAAGTTATAAAATTGAATTATTAGAAGATACCGAAGTTAAAAATAATAAGTTTATTAAACCTATTGTGTTAAAAGGAAAATTTAAATGGAg                                                        >  1:742613/1‑251 (MQ=255)                                                                                                                                                         gaataTCTCTTACTGGTATTAGAAAAAACAGTAAGAGTTCAAATGGTTTAATGAATCAGACAAACAATATAGGGGTTCTTACATTTCCTTCAGATATTATTACTACAAAATTACCAGATGGGATTTATAAGGCTGGTATGTACgg                                                                                                                                                        >  1:625393/1‑145 (MQ=255)                                                                                                                                                               ctctTACTGGTATTAGAAAAAACAGTAAGAGTTCAAATGGTTTAATGAATCAGACAAACAATATAGGGGTTCTTACATTTCCTTCAGATATTATTACTACAAAATTACCAGATGGGATTTATAAGGCTGGTATGTACGGCACATCAAGTTATAAAATTGAATTATTagaaga                                                                                                                       >  1:859345/1‑172 (MQ=255)                                                                                                                                                                         tATTAGAAAAAACAGTAAGAGTTCAAATGGTTTAATGAATCAGACAAACAATATAGGGGTTCTTACATTTCCTTCAGATATGATTACTACAAAATTACCAGATGGGATTTATAAGGCTGGTATGTACGGCACATCAAGTTATAAAATTGAATTATTAGAAGATACCGAAGTTAAAAATAATAAGTTTATTAAACCTATTGTGTTAAAAGGAAAATTTAAATGGAGCCAAAAATATTTGGATGATtcaatca                              <  1:442962/251‑1 (MQ=255)                                                                                                                                                                                           gagTTCAAATGGTTTAATGAATCAGACAAACAATATAGGGGTTCTTACATTTCCTTCAGATATTATTACTACAAAATTACCAGATGGGATTTATAAGGCTGGTATGTACGGCACATCAAGTTATAAAATTGAATTATTAGAAGATACCGAAGTTAAAAATAATAAGTTTATTAAACCTATTGTGTTAAAAGGAAAATTTAAATGGAGCCAAAAATATTTGGATGATTCAATCAAGAGTGGAGTAAAgg               >  1:749614/1‑248 (MQ=255)                                                                                                                                                                                                      gTTTAATGAATCAGACAAACAATATAGGGGTTCTTACATTTCCTTCAGATATTATTACTACAAAATTACCAGATGGGATTTATAAGGCTGGTATGTACGGCACATCAAGCTATAAAATTGAATTATTAGAAGATACCGAAGTTAAAAATAATAAGTTTATTAAACCTATTGTGTTAAAAGGAAAATTTAAATGGAGCCAAAAATATTTGGATGATTCAATCAAGAGTGGAGTAAAGGTTATTATTCAAAc  >  1:796758/1‑250 (MQ=255)                                                                                                                                                                                                                   gACAAACAATATAGGGGTTCTTACATTTCCTTCAGATATTATTACTACAAAATTACCAGATGGGATTtata                                                                                                                                                                        <  1:780540/71‑1 (MQ=255)                                                                                                                                                                                                                         caaTATAGGGGTTCTTACATTTCCTTCAGATATTATTACTACAAAATTACCAGATGGGATTTATAAGGCTGGTATGTACGGCACATCAAGTTATAAAATTGAat                                                                                                                                 >  1:69099/1‑104 (MQ=255)                                                                                                                                                                                                                         caaTATAGGGGTTCTTACATTTCCTTCAGATATTATTACTACAAAATTACCAGATGGGATTTATAAGGCTGGTATGTACGGCACATCAAGTTATAAAATTGAATTATTAGAAGATACCGAAGTTAAAAATAATAAGTTTATTAAACCTATTGTGTTaaaa                                                                         >  1:266782/1‑160 (MQ=255)                                                                                                                                                                                                                           atatAGGGGTTCTTACATTTCCTTCAGATATTATTACTACAAAATTACCAGATGGGATTTATAAGGCTGGTATGTACGGCACATCAAGTTATAAAATTGAATTATTAGAAGATACCGAAGt                                                                                                              >  1:274843/1‑121 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                      GATTATCAATCTGAAAAATATTTTAGATTATACTCTAGGTAAAGATAACTTTATTGATATTTTTTCTTGGCAAAAGACAACCACACCACCTAATTTATCTAAACAAACAAAACAATCTTGCGAATACATTTTAGCTTATCAAAGTGAGAATAGAATATCTCTTACTGGTATTAGAAAAAACAGTAAGAGTTCAAATGGTTTAATGAATCAGACAAACAATATAGGGGTTCTTAAATTTCCTTCAGATATTATTACTACAAAATTACCAGATGGGATTTATAAGGCTGGTATGTGCGGCACATCAAGTTATAAAATTGAATTATTAGAAGATACCGAAGTTAAAAATAATAAGTTTATTAAACCTATTGTGTTAAAAGGAAAATTTAAATGGAGCCAAAAATATTTGGATGATTCAATCAAGAGTGGAGTAAAGGTTATTATTCAAAC  >  1/1144994‑1145440

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑