Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,142,257	A→G	intergenic (+3127/+251054)	unknown → / ← unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,142,257	0	A	G	100.0%	238.4 / NA	79	intergenic (+3127/+251054)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (38/41);  total (38/41)

GAACCGTGATGTGGTGATCGGCGAAACCATTACTGTTGCCGAACTTGCCAATAAAATGGCGGTAAAAGCCACTGAAGTGATTAAAACCATGATGAAAATGGGCGCCATGGCGACTATCAACCAAGTGATCGACCAAGAAACCGCACAATTGGTGGCGGAAGAAATGGGTCACAAAGTGATCGTGCGTAAAGAAAACGAGTTGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTAGTCACCCGCGCACCGGTGGTGACTATCATGGGGCACGTTGACCATGGTAAAACCTCCTTACTTGACTATATTCGTAAAGCCAAAGTGGCGGCAGGCGAAGCAGGCGGGATCACGCAACATATCGGTGCGTACCATGTGGAAACCGACGACGGCAAAATGATCACTTTCTTAGATACGCCAGGACATGCGGCGTTTACTTCCATGCGTGCGCGTGGTGCGAAAGCGACGGACATCGTGGTGC  >  1/1142014‑1142498                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                  gAACCGTGATGTGGTGATCGGCGAAACCATTACTGTTGCCGAACTTGCCAATAAAATGGCGGTAAAAGCCACTGAAGTGATTAAAACCATGATGAAAATGGGCGCCATGGCGACTATCAACCAAGTGATCGACCAAGAAACCGCACAATTGGTGGCGGAAGAAATGGGTCACAAAGTGATCGTGCGTAAAGAAAACGAGTTGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTGGTc                                                                                                                                                                                                                                                >  1:849399/1‑247 (MQ=255) gAACCGTGATGTGGTGATCGGCGAAACCATTACTGTTGCCGAACTTGCCAATAAAATGGCGGTAAAAGCCACTGAAGTGATTAAAACCATGATGAAAATGGGCGCCATGGCGACTATCAACCAAGTGATCGACCAAGAAACCGCACAATTGGTGGCGGAAGAAATGGGTCACAAAGTGATCGTGCGTAAAGAAAACGAGTTGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTGGTCAcc                                                                                                                                                                                                                                             >  1:251617/1‑250 (MQ=255)         aTGTGGTGATCGGCGAAACCATTACTGTTGCCGAACTTGCCAATAAAATGGCGGTAAAAGCCACTGAAGGGATTAAAAACATGATGAAAATGGGCGCCATGGCGACTATCAACCAAGTGATCGACCAAGAAACCGCACAATTGGTGGCGGAAGAAATGGGGCACAAAGTGATCGTGCGTAAAGAAAACGAGTTGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTGGGCAACCGCGCACCg                                                                                                                                                                                                                                    >  1:30997/1‑251 (MQ=255)                 aTCGGCGAAACCATTACTGTTGCCGAACTTGCCAATAAAATGGCGGTAAAAGCCACTGAAGTGATTAAAACCATGATGAAAATGGGCGCCATGGCGACTATCAACCAAGTGATCGACCAAGAAACCGCACAATTGGTGGCGGAAGAAATGGGTCACAAAGTGATCGTGCGTAAAGAAAACGAGTTGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGAc                                                                                                                                                                                                                            <  1:320336/251‑1 (MQ=255)                      cGAAACCATTACTGTTGCCGAACTTGCCAATAAAATGGCGGTAAAAGCCACTGAAGTGATTAAAACCATGATGAAAATGGGCGCCATGGCGACTATCAACCAAGTGATCGACCAAGAAACCGCACAATTGGTGGCGGAAGAAATGGGTCACAAAGTGATCGTGCGTAAAGAAAACGAGTTGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCa                                                                                                                                                                                                                       <  1:772525/251‑1 (MQ=255)                         aaCCATTACTGTTGCCGAACTTGCCAATAAAATGGCGGTAAAAGCCACTGAAGTGATTAAAACCATGATGAAAATGGGCGCCATGGCGACTATCAACCAAGTGATCGACCAAGAAACCGCACAATTGGTGGCGGAAGAAATGGGTCACAAAGTGATCGTGCGTAAAGAAAACGAGTTGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATgg                                                                                                                                                                                                                    <  1:581866/251‑1 (MQ=255)                           ccATTACTGTTGCCGAACTTGCCAATAAAATGGCGGTAAAAGCCACTGAAGTGATTAAAACCATGATGAAAATGGGCGCCATGGCGACTATCAACCAAGTGATCGACCAAGAAACCGCACAATTGGTGGCGGAAGAAATGGGTCACAAAGTGATCGTGCGTAAAGAAAACGAGTTGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATgggg                                                                                                                                                                                                                  >  1:862096/1‑251 (MQ=255)                            cATTACTGTTGCCGAACTTGCCAATAAAATGGCGGTAAAAGCCACTGAAGTGATTAAAACCATGATGAAAATGGGCGCCATGGCGACTATCAACCAAGTGATCGACCAAGAAATCGCACAATTGGTGGCGGAAGAAATGGGTCACAAAGTGATCGTGCGTAAAGAAAACAAGTTGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGCGGTGACTATCATtggg                                                                                                                                                                                                                  >  1:446404/1‑250 (MQ=255)                                aCTGTTGCCGAACTTGCCAATAAAATGGCGGTAAAAGCCACTGAAGTGATTAAAACCATGATGAAAATGGGCGCCATGGCGACTATCAACCAAGTGATCGACCAAGAAACCGCACAATTGGTGGCGGAAGAAATGGGTCACAAAGTGATCGTGCGTAAAGAAAACGAGTTGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATGGGGCACGt                                                                                                                                                                                                             <  1:152050/251‑1 (MQ=255)                                aCTGTTGCCGAACTTGCCAATAAAATGGCGGTAAAAGCCACTGAAGTGATTAAAACCATGATGAAAATGGGCGCCATGGCGACTATCAACCAAGTGATCGACCAAGAAACCGCACAATTGGTGGCGGAAGAAATGGGTCACAAAGTGATCGTGCGTAAAGAAAACGAGTTGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATGGGGCACGt                                                                                                                                                                                                             <  1:357049/251‑1 (MQ=255)                                 cTGTTGCCGAACTTGCCAATAAAATGGCGGTAAAAGCCACTGAAGTGATTAAAACCATGATGAAAATTGGCGCCATGGCGACTATCAACCAAGTGATCGACCAAGAAACCGCACAATTGGTGGCGGAAGAAATGGGTCACAAAGTGATCGTGCGTAAAGAAAACGAGTTGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATGGGGCACGtt                                                                                                                                                                                                            >  1:46212/1‑251 (MQ=255)                                    ttGCCGAACTTGCCAATAAAATGGCGGTAAAAGCCACTGAAGTGATTAAAACCATGATGAAAATGGGCGCCATGGCGACTATCAACCAAGTGATCGACCAAGAAACCGCACAATTGGTGGCGGAAGAAATGGGTCACAAAGTGATCGTGCGTAAAGAAAACGAGTTGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATGGGGCACGTTGAc                                                                                                                                                                                                         <  1:674561/251‑1 (MQ=255)                                                 cAATAAAATGGCGGTAAAAGCCACTGAAGTGATTAAAACCATGATGAAAATGGGCGCCATGGCGACTATCAACCAAGTGATCGACCAAGAAACCGCACAATTGGTGGCGGAAGAAATGGGTCACAAAGTGATCGTGCGTAAAGAAAACGAGTTGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATGGGGCACGTTGACCATGGTAAAAcct                                                                                                                                                                                            <  1:625308/251‑1 (MQ=255)                                                   aTAAAATGGCGGTAAAAGCCACTGAAGTGATTAAAACCATGATGAAAATGGGCGCCATGGCGACTATCAACCAAGTGATCGACCAAGAAACCGCACAATTGGTGGCGGAAGAAATGGGTCACAAAGTGATCGTGCGTAAAGAAAACGAGTTGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATGGGGCACGTTGACCATGGTAAAAcctc                                                                                                                                                                                           >  1:404298/1‑250 (MQ=255)                                                     aaaaTGGCGGTAAAAGCCACTGAAGTGATTAAAACCATGATGAAAATGGGCGCCATGGCGACTATCAACCAAGTGATCGACCAAGAAACCGCACAATTGGTGGCGGAAGAAATGGGTCACAAAGTGATCGTGCGTAAAGAAAACGAGTTGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATGGGGCACGTTGACCATGGTAAAACCTCCtt                                                                                                                                                                                        <  1:619200/251‑1 (MQ=255)                                                              gTAAAAGCCACTGAAGTGATTAAAACCATGATGAAAATGGGCGCCATGGCGACTATCAACCAAGTGATCGACCAAGAAACCGCACAATTGGTGGCGGAAGAAATGGGTCACAAAGTGATCGTGCGTAAAGAAAACGAGTTGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATGGGGCACGTTGACCATGGTAAAACCTCCTTAc                                                                                                                                                                                      >  1:650098/1‑244 (MQ=255)                                                                 aaaGCCACTGAAGTGATTAAAACCATGATGAAAATGGGCGCCATGGCGACTATCAACCAAGTGATCGACCAAGAAACCGCACAATTGGTGGCGGAAGAAATGGGTCACAAAGTGATCGTGCGTAAAGAAAACGAGTTGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATGGGGCACGTTGACCATGGTAAAACCTCCTTACTTGACTatat                                                                                                                                                                            <  1:776293/251‑1 (MQ=255)                                                                  aaGCCACTGAAGTGATTAAAACCATGATGAAAATGGGCGCCATGGCGACTATCAACCAAGTGATCGACCAAGAAACCGCACAATTGGTGGCGGAAGAAATGGGTCACAAAGTGATCGTGCGTAAAGAAAACGAGTTGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCggtgg                                                                                                                                                                                                                                >  1:596436/1‑198 (MQ=255)                                                                  aaGCCACTGAAGTGATTAAAACCATGATGAAAATGGGCGCCATGGCGACTATCAACCAAGGGATCGACCAAGAAACCGCACAATTGGTGGCGGAAGAAATGGGTCACAAAGTGATCGTGCGTAAAGAAAACGAGTTGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATGGGGCACGTTGACCATGGTAAAACCTCCTTACTTGACTATAtt                                                                                                                                                                           <  1:287236/251‑1 (MQ=255)                                                                     ccACTGAAGTGATTAAAACCATGATGAAAATGGGCGCCATGGCGACTATCAACCAAGTGATCGACCAAGAAACCGCACAATTGGTGGCGGAAGAAATGGGTCACAAAGTGATCGTGCGTAAAGAAAACGAGTTGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATGGGGCACGTTGACCa                                                                                                                                                                                                       >  1:111857/1‑220 (MQ=255)                                                                     ccACTGAAGTGATTAAAACCATGATGAAAATGGGCGCCATGGCGACTATCAACCAAGTGATCGACCAAGAAACCGCACAATTGGTGGCGGAAGAAATGGGTCACAAAGTGATCGTGCGTAAAGAAAACGAGTTGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATGGGGCACGTTGACCATGGTAAAAcc                                                                                                                                                                                             <  1:331593/230‑1 (MQ=255)                                                                     ccACTGAAGTGATTAAAACCATGATGAAAATGGGCGCCATGGCGACTATCAACCAAGTGATCGACCAAGAAACCGCACAATTGGTGGCGGAAGAAATGGGTCACAAAGTGATCGTGCGTAAAGAAAACGAGTTGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATGGGGCACGTTGACCATGGTAAAACCTCCTTACTTGACTATATTCGt                                                                                                                                                                        >  1:637662/1‑251 (MQ=255)                                                                     ccACTGAAGTGATTAAAACCATGATGAAAATGGGCGCCATGGCGACTATCAACCAAGTGATCGACCAAGAAACCGCACAATTGGTGGCGGAAGAAATGGGTCACAAAGTGATCGTGCGTAAAGAAAACGAGTTGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATGGGGCACGTTGACCATGGTAAAACCTCCTTACTTGACTATATTCGt                                                                                                                                                                        >  1:369400/1‑251 (MQ=255)                                                                          gAAGTGATTAAAACCATGATGAAAATGGGCGCCATGGCGACTATCAACCAAGTGATCGACCAAGAAACCGCACAATTGGTGGCGGAAGAAATGGGTCACAAAGTGATCGTGCGTAAAGAAAACGAGTTGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATGGGGCACGTTGAcc                                                                                                                                                                                                        <  1:650683/214‑1 (MQ=255)                                                                          gAAGTGATTAAAACCATGATGAAAATGGGCGCCATGGCGACTATCAACCAAGTGATCGACCAAGAAACCGCACAATTGGTGGCGGAAGAAATGGGTCACAAAGTGATCGTGCGTAAAGAAAACGAGTTGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATGGGGCACGTTGAcc                                                                                                                                                                                                        >  1:688682/1‑214 (MQ=255)                                                                          gAAGTGATTAAAACCATGATGAAAATGGGCGCCATGGCGACTATCAACCAAGTGATCGACCAAGAAACCGCACAATTGGTGGCGGAAGAAATGGGTCACAAAGTGATCGTGCGTAAAGAAAACGAGTTGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATGGGGCACGTTGACCATGGTAAAACCTCCTTACTTGACTATATTCGTAAAg                                                                                                                                                                    >  1:71988/1‑250 (MQ=255)                                                                               gATTAAAACCATGATGAAAATGGGCGCCATGGCGACTATCAACCAAGTGATCGACCAAGAAACCGCACAATTGGTGGCGGAAGAAATGGGTCACAAAGTGATCGTGCGTAAAGAAAACGAGTTGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTGGTc                                                                                                                                                                                                                                                >  1:422280/1‑169 (MQ=255)                                                                               gATTAAAACCATGATGAAAATGGGCGCCATGGCGACTATCAACCAAGTGATCGACCAAGAAACCGCACAATTGGTGGCGGAAGAAATGGGTCACAAAGTGATCGTGCGTAAAGAAAACGAGTTGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATGGGGc                                                                                                                                                                                                                 <  1:331175/200‑1 (MQ=255)                                                                               gATTAAAACCATGATGAAAATGGGCGCCATGGCGACTATCAACCAAGTGATCGACCAAGAAACCGCACAATTGGTGGCGGAAGAAATGGGTCACAAAGTGATCGTGCGTAAAGAAAACGAGTTGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATGGGGCACGTTGACCATGGTaa                                                                                                                                                                                                 <  1:432857/216‑1 (MQ=255)                                                                                aTTAAAACCATGATGAAAATGGGCGCCATGGCGACTATCAACCAAGTGATCGACCAAGAAACCGCACAATTGGTGGCGGAAGAAATGGGTCACAAAGTGATCGTGCGTAAAGAAAACGAGTTGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATGGGGCACGTTGACCATGGTAAAACCTCCtt                                                                                                                                                                                        <  1:206283/224‑1 (MQ=255)                                                                                  tAAAACCATGATGAAAATGGGCGCCATGGCGACTATCAACCAAGTGATCGACCAAGAAACCGCACAATTGGTGGCGGAAGAAATGGGTCACAAAGTGATCGTGCGTAAAGAAAACGAGTTGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATGGGGCACGTTGACCATGGTAAAACCTCCTTACTTGACTATATTCGTAAAGCCAAAGTgg                                                                                                                                                           <  1:735434/251‑1 (MQ=255)                                                                                     aaCCATGATGAAAATGGGCGCCATGGCGACTATCAACCAAGTGATCGACCAAGAAACCGCACAATTGGTGGCGGAAGAAATGGGTCACAAAGTGATCGTGCGTAAAGAAAACGAGTTGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATGGGGCACGTTGACCATGGTAAAACCTCCTTACTTGACTATATTCGTAAAGCCAAAGTggcgg                                                                                                                                                        <  1:891547/251‑1 (MQ=255)                                                                                      aCCATGATGAAAATGGGCGCCATGGCGACTATCAACCAAGTGATCGACCAAGAAACCGCACAATTGGTGGCGGAAGAAATGGGTCACAAAGTGATCGTGCGTAAAGAAAACGAGTTGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATgg                                                                                                                                                                                                                    >  1:686794/1‑190 (MQ=255)                                                                                       ccATGATGAAAATGGGCGCCATGGCGACTATCAACCAAGTGATCGACCAAGAAACCGCACAATTGGTGGCGGAAGAAATGGGTCACAAAGTGATCGTGCGTAAAGAAAACGAGTTGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATGGGGCACGTTGACCATGGTAAAACCTCCTTACTTGACTATATTCGTAAAGCCAAAGTGGCGGCa                                                                                                                                                      >  1:299011/1‑251 (MQ=255)                                                                                        cATGATGAAAATGGGCGCCATGGCGACTATCAACCAAGTGATCGACCAAGAAACCGCACAATTGGTGGCGGAAGAAATGGGTCACAAAGTGATCGTGCGTAAAGAAAACGAGTTGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATGGGGc                                                                                                                                                                                                                 >  1:214364/1‑191 (MQ=255)                                                                                            atgaAAATGGGCGCCATGGCGACTATCAACCAAGTGATCGACCAAGAAACCGCACAATTGGTGGCGGAAGAAATGGGTCACAAAGTGATCGTGCGTAAAGAAAACGAGTTGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATGGGGCACGTTGACCATGGTAAAACCTCCTTACTTGACTatat                                                                                                                                                                            >  1:360316/1‑224 (MQ=255)                                                                                              gaAAATGGGCGCCATGGCGACTATCAACCAAGTGATCGACCAAGAAACCGCACAATTGGTGGCGGAAGAAATGGGTCACAAAGTGATCGTGCGTAAAGAAAACGAGTTGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATGGGGCACGTTGAcc                                                                                                                                                                                                        >  1:370060/1‑194 (MQ=255)                                                                                              gaAAATGGGCGCCATGGCGACTATCAACCAAGTGATCGACCAAGAAACCGCACAATTGGTGGCGGAAGAAATGGGTCACAAAGTGATCGTGCGTAAAGAAAACGAGTTGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATGGGGCACGTTGACCATGGTAAAACCTCCTTACTTGACTATATTCGTAAAGCCAAAGTGGCGGCAGGCGAAg                                                                                                                                               >  1:120333/1‑251 (MQ=255)                                                                                                  aTGGGCGCCATGGCGACTATCAACCAAGTGATCGACCAAGAAACCGCACAATTGGTGGCGGAAGAAATGGGTCACAAAGTGATCGTGCGTAAAGAAAACGAGTTGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATGGGGCACGTTGACCATGGTAAAAc                                                                                                                                                                                              <  1:37905/200‑1 (MQ=255)                                                                                                             ggCGACTATCAACCAAGTGATCGACCAAGAAACCGCACAATTGGTGGCGGAAGAAATGGGTCACAAAGTGATCGTGCGTAAAGAAAACGAGTTGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATGGGGCACGTTg                                                                                                                                                                                                           <  1:354312/176‑1 (MQ=255)                                                                                                             ggCGACTATCAACCAAGTGATCGACCAAGAAACCGCACAATTGGTGGCGGAAGAAATGGGTCACAAAGTGATCGTGCGTAAAGAAAACGAGTTGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATGGGGCACGTTGACCATGGTAAAACCTCCTTACTTGACTATATTCGTAAAGCCAAAGTGGCGGCAGGCGAAGCAGGCGGGATCACg                                                                                                                                 >  1:796014/1‑250 (MQ=255)                                                                                                                 aCTATCAACCAAGTGATCGACCAAGAAACCGCACAATTGGTGGCGGAAGAAATGGGTCACAAAGTGATCGTGCGTAAAGAAAACGAGTTGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATGGGGCACGTTGACCATGGTAAAACCTCCTTACTTGACTATATTCGTAAAGCCAAAGTGGCGGCAGGCGAAGCAGGCGGGATCACGCAACa                                                                                                                            >  1:740984/1‑251 (MQ=255)                                                                                                                    aTCAACCAAGTGATCGACCAAGAAACCGCACAATTGGTGGCGGAAGAAATGGGTCACAAAGTGATCGTGCGTAAAGAAAACGAGTTGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATGGGGCACGTTGACCATGGTAAAACCTCCTTACTTGACTATATTCGTAAAGCCAAAGTGGCGGCAGGCGAAGCAGGCGGGATCACGCAACatat                                                                                                                         >  1:718827/1‑251 (MQ=255)                                                                                                                       aaCCAAGTGATCGCCCAAGAAACCGCACAATTGGTGGCGGAAGAAATGGGTCACAAAGTGATCGTGCGTAAAGAAAACGAGTTGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATGGGGCACGTTGACCATGGTAAAACCTCCTTACTTGACTATATTCGTAAAGCCAAAGTGGCGGCAGGCGAAGCAGGCGGGATCACGCAACATATCgg                                                                                                                      <  1:108203/251‑1 (MQ=255)                                                                                                                       aaCCAAGTGATCGACCAAGAAACCGCACAATTGGTGGCGGAAGAAATGGGTCACAAAGTGATCGTGCGTAAAGAAAACGAGTTGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATGGGGCACGTTGACCATGGTAAAACCTCCTTACTTGACTATATTCGTAAAGCCAAAGTGGCGGCAGGCGAAGCAGGCGGGATCACGCAACATATCgg                                                                                                                      <  1:533930/251‑1 (MQ=255)                                                                                                                         ccAAGTGATCGACCAAGAAACCGCACAATTGGTGGCGGAAGAAATGGGTCACAAAGTGATCGTGCGTAAAGAAAACGAGTTGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATGGGGCACGTTGACCATGGTAAAACCTCCTTACTTGAc                                                                                                                                                                                 <  1:75478/190‑1 (MQ=255)                                                                                                                              tGATCGACCAAGAAACCGCACAATTGGTGGCGGAAGAAATGGGTCACAAAGTGATCGTGCGTAAAGAAAACGAGTTGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATGGGGCACGTTGACCATGGTAAAACCTCCTTACTTGACTATATTCGTAAAGCCAAAGTGGCGGCAGGCGAAGCAGGCGGGATCACGCAACATATCGGTGCGTAc                                                                                                               <  1:253171/251‑1 (MQ=255)                                                                                                                                aTCGACCAAGAAACCGCACAATTGGTGGCGGAAGAAATGGGTCACAAAGTGATCGTGCGTAAAGAAAACGAGTTGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATGGGGCACGTTGACCATGGTAAAACCTCCTTACTTGACTATATTCGTAAAGCCAAAGTGGCGGCAGGCGAAGCAGGCGGGATCACGCAACATATCGGTGCGTACCa                                                                                                             <  1:484950/251‑1 (MQ=255)                                                                                                                                   gACCAAGAAACCGCACAATTGGTGGCGGAAGAAATGGGTCACAAAGTGATCGTGCGTAAAGAAAACGAGTTGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCAt                                                                                                                                                                                                                      <  1:80009/143‑1 (MQ=255)                                                                                                                                     ccAAGAAACCGCACAATTGGTGGCGGAAGAAATGGGTCACAAAGTGATCGTGCGTAAAGAAAACGAGTTGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCgg                                                                                                                                                                                                                                   <  1:840574/128‑1 (MQ=255)                                                                                                                                     ccAAGAAACCGCACAATTGGTGGCGGAAGAAATGGGTCACAAAGTGATCGTGCGTAAAGAAAACGAGTTGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATGGGGCACGTTGACCATGGTAAAACCTCCTTACTTGACTATATTCGTAAAGCCAAAGTGGCGGCAGGCGAAGCAGGCGGGATCACGCAACATATCGGTGCGTACCATGTgg                                                                                                        <  1:513539/251‑1 (MQ=255)                                                                                                                                                           gCGGAAGAAATGGGTCACAAAGTGATCGTGCGTAAAGAAAACGAGTTGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATGGGGCACGTTGACCATGGTAAAACCTCCTTACTTGACTATATTCGTAAAGCCAAAGTGGCGGCAGGCGAAGCAGGCGGGATCACGCAACATATCGGTGCGTACCATGTGGAAACCGACGACGGCAAAATGa                                                                                   <  1:594658/250‑1 (MQ=255)                                                                                                                                                                    aTGGGTCACAAAGTGATCGTGCGTAAAGAAAACGAGTTGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATGGGGCACGTTGACCATGGTAAAACCTCCTTACTTGACTATATTCGTAAAGCCAAAGTGGCGGCAGGCGAAGCAGGCGGGATCACGCAACATATCGGTGCGTACCATGTGGAAACCGACGACGGCAAAATGATCACTTTCtt                                                                         >  1:630245/1‑251 (MQ=255)                                                                                                                                                                        gTCACAAAGTGATCGTGCGTAAAGAAAACGAGTTGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACt                                                                                                                                                                                                                           <  1:154927/101‑1 (MQ=255)                                                                                                                                                                            caAAGTGATCGTGCGTAAAGAAAACGAGTTGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATGGGGCACGTTGACCATGGTAAAAc                                                                                                                                                                                              >  1:773995/1‑126 (MQ=255)                                                                                                                                                                            caAAGTGATCGTGCGTAAAGAAAACGAGTTGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATGGGGCACGTTGACCATGGTAAAACCTCCTTACTTGACt                                                                                                                                                                                >  1:199058/1‑140 (MQ=255)                                                                                                                                                                            caAAGTGATCGTGCGTAAAGAAAACGAGTTGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATGGGGCACGTTGACCATGGTAAAACCTCCTTACTTGACTATATTCGTAAAGCCAAAGTGGCGGCAGGCGAAGCAGGCGGGATCACGCAACATATCGGTGCGTAcc                                                                                                              <  1:566876/206‑1 (MQ=255)                                                                                                                                                                                gTGATCGTGCGTAAAGAAAACGAGTTGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATGGGGCACGTTGACCATGGt                                                                                                                                                                                                   <  1:222000/117‑1 (MQ=255)                                                                                                                                                                                gTGATCGTGCGTAAAGAAAACGAGTTGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATGGGGCACGTTGACCATGGTAAAACCTCCTTAc                                                                                                                                                                                      <  1:589977/130‑1 (MQ=255)                                                                                                                                                                                   aTCGTGCGTAAAGAAAACGAGTTGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATGGGGCACGTTGACCATGGTAAAACCTCCTTACTTGACTATATTCGTAAAGCCAAAGTGGCGGCAGGCGAAGCAGGCGGGATCACGCAACATATCGGTGCGTAc                                                                                                               <  1:688335/198‑1 (MQ=255)                                                                                                                                                                                        gCGTAAAGAAAACGAGTTGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATGGGGCACGTTGACCATGGTAAAACCTCCTTACTTGACTATATTCGTAAAGCCAAAGTGGCGGCAGGCGAAGCAGGCGGGATCACGCAACATATc                                                                                                                        >  1:639505/1‑184 (MQ=255)                                                                                                                                                                                               gAAAACGAGTTGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATGGGGCACGTTGACCATGGTAAAACCTCCTTAc                                                                                                                                                                                      >  1:723459/1‑115 (MQ=255)                                                                                                                                                                                                  aaCGAGTTGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATGGGGCACGTTGACCATGGTAAAACCTCCTTACTTGACTATATTCGTAAAGCCAAAGTGGCGGCAGGCGAAGCAGGCGGGATCACGCAACATATCGGTGCGTACCATGTGGAAACCGACGACGGCAAAATGATCACTTTCTTAGATACGCCAGGACATGCGGCGTTTACTTc                                           <  1:247374/251‑1 (MQ=255)                                                                                                                                                                                                      aGTTGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATGGGGCACGTTGACCATGGTAAAACCTCCTTACTTGACTATATTCGTAAAGCCAAAGTGGCGGCAGGCGAAGCAGGCGGGATCACGCAACATATCGGTGCGTACCATGTGGAAACCGACGACGGCAAAATGATCACTTTCTTAGATACGCCAGGACa                                                          >  1:148164/1‑232 (MQ=255)                                                                                                                                                                                                        ttGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATGGGGCACGTTGACCATGGTAAAACCTCCTTACTTGACTATATTCGTAAAGCCAAAGTGGCGGCAGGCGAAGCAGGCGGGATCACGCAACATATCGGTGCGTACCATGTGGAAACCGACGACGGCAAAATGATCACTTTCTTAGATACGCCAGGACATGCGGCGTTTACTTCCAtg                                       <  1:281520/249‑1 (MQ=255)                                                                                                                                                                                                                    gTCATGAGCGATCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATGGGGCACGTTGACCATGGTAAAACCTCCTTACTTGACTATATTCGTa                                                                                                                                                                       >  1:451102/1‑109 (MQ=255)                                                                                                                                                                                                                          aGCGATCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATGGGGCACGTTGACCATGGTAAAACCTCCTTACTTGACTATATTCGTAAAGCCAAAGTGGCGGCAGGCGAAGCAGGCGGGATCACGCAACATATCGGTGCGTACCATGTGGAAACCGACGACGGCAAAATGATCACTTTCTTAGATACGCCAGGACATGCGGCGTTTACTTCCATGCGTGCGCGTGGTGCGAAAg                    <  1:477929/250‑1 (MQ=255)                                                                                                                                                                                                                              aTCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATGGGGCACGTTGACCATGGTAAAACCTCCTTACTTGACTATATTCGTAAAGCCAAAGTGGCGGCAGGCGAAGCAGGCGGGATCACGCAACATATCgg                                                                                                                      >  1:502625/1‑148 (MQ=255)                                                                                                                                                                                                                              aTCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATGGGGCACGTTGACCATGGTAAAACCTCCTTACTTGACTATATTCGTAAAGCCAAAGTGGCGGCAGGCGAAGCAGGCGGGATCACGCAACATATCGGTGCGTACCATGTGGAAACCGACGACGGCAAAATGATCACTTTCTTAGATACGcc                                                                >  1:144251/1‑202 (MQ=255)                                                                                                                                                                                                                              aTCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATGGGGCACGTTGACCATGGTAAAACCTCCTTACTTGACTATATTCGTAAAGCCAAAGTGGCGGCAGGCGAAGCAGGCGGGATCACGCAACATATCGGTGCGTACCATGTGGAAACCGACGACGGCAAAATGATCACTTTCTTAGATACGCCAGGAc                                                           <  1:752582/207‑1 (MQ=255)                                                                                                                                                                                                                              aTCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATGGGGCACGTTGACCATGGTAAAACCTCCTTACTTGACTATATTCGTAAAGCCAAAGTGGCGGCAGGCGAAGCAGGCGGGATCACGCAACATATCGGTGCGTACCATGTGGAAACCGACGACGGCAAAATGATCACTTTCTTAGATACGCCAGGACATGCGGCGTTTACTTc                                           <  1:716513/223‑1 (MQ=255)                                                                                                                                                                                                                              aTCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATGGGGCACGTTGACCATGGTAAAACCTCCTTACTTGACTATATTCGTAAAGCCAAAGTGGCGGCAGGCGAAGCAGGCGGGATCACGCAACATATCGGTGCGTACCATGTGGAAACCGACGACGGCAAAATGATCACTTTCTTAGATACGCCAGGACATGCGGCGTTTACTTCCATGCGTGCGCGTGGTGCGaa                      <  1:607216/244‑1 (MQ=255)                                                                                                                                                                                                                              aTCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATGGGGCACGTTGACCATGGTAAAACCTCCTTACTTGACTATATTCGTAAAGCCAAAGTGGCGGCAGGCGAAGCAGGCGGGATCACGCAACATATCGGTGCGTACCATGTGGAAACCGACGACGGCAAAATGATCACTTTCTTAGATACGCCAGGACATGCGGCGTTTACTTCCATGCGTGCGCGTGGTGCGAAAGCGACg               >  1:488944/1‑251 (MQ=255)                                                                                                                                                                                                                              aTCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATGGGGCACGTTGACCATGGTAAAACCTCCTTACTTGACTATATTCGTAAAGCCAAAGTGGCGGCAGGCGAAGCAGGCGGGATCACGCAACATATCGGTGCGTACCATGTGGAAACCGACGACGGCAAAATGATCACTTTCTTAGATACGCCAGGACATGCGGCGTTTACTTCCATGCGTGCGCGTGGTGCGAAAGCGACg               >  1:798091/1‑251 (MQ=255)                                                                                                                                                                                                                              aTCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATGGGGCACGTTGACCATGGTAAAACCTCCTTACTTGACTATATTCGTAAAGCCAAAGTGGCGGCAGGCGAAGCAGGCGGGATCACGCAACATATCGGTGCGTACCATGTGGAAACCGACGACGGCAAAATGATCACTTTCTTAGATACGCCAGGACATGCGGCGTTTACTTCCATGCGTGCGCGTGGTGCGAAAGCGACg               >  1:714607/1‑251 (MQ=255)                                                                                                                                                                                                                               tCGTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATGGGGCACGTTGACCATGGTAAAACCTCCTTACTTGACTATATTCGTAAAGCCAAAGTGGCGGCAGGCGAAGCAGGCGGGATCACGCAACATATCGGTGCGTACCATGTGGAAACCGACGACGGCAAAATGATCACTTTCTTAGATACGcc                                                                >  1:769842/1‑201 (MQ=255)                                                                                                                                                                                                                                 gTGATGTGGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATGGGGCACGTTGACCATGGTAAAAcc                                                                                                                                                                                             <  1:291787/74‑1 (MQ=255)                                                                                                                                                                                                                                      gtgGACGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATGGGGCACGTTGACCATGGTAAAACCTCCTTACTTGACTATATTCGTAAAGCCAAAGTGGCGGCAGGCGAAGCAGGCGGGATCACGCAACATATCGGTGCGTACCATGTGGAAACCGACGACGGCAAAATGATCACTTTCTTAGATACGCCAGGACATGCTGCGTCTACTTCCATGCGTGCGCGTGGTGCGAAAGCGACGGACATCgt       >  1:54566/1‑251 (MQ=255)                                                                                                                                                                                                                                           cGCCGAATTGGTCACCCGCGCACCGGTGGTGACTATCATGGGGCACGTTGACCATGGTAAAACCTCCTTACTTGACTATATTCGTAAAGCCAAAGTGGCGGCAGGCGAAGCAGGCGGGATCACGCAACATATCGGTGCGTACCATGTGGAAACCGACGACGGCAAAATGATCACTTTCTTAGATACGCCAGGACATGCGGCGTTTACTTCCATGCGTGCGCGTGGTGCGAAAGCGACGGACATCGTGGTGc  <  1:56278/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                  GAACCGTGATGTGGTGATCGGCGAAACCATTACTGTTGCCGAACTTGCCAATAAAATGGCGGTAAAAGCCACTGAAGTGATTAAAACCATGATGAAAATGGGCGCCATGGCGACTATCAACCAAGTGATCGACCAAGAAACCGCACAATTGGTGGCGGAAGAAATGGGTCACAAAGTGATCGTGCGTAAAGAAAACGAGTTGGAAGAAGCGGTCATGAGCGATCGTGATGTGGACGCCGAATTAGTCACCCGCGCACCGGTGGTGACTATCATGGGGCACGTTGACCATGGTAAAACCTCCTTACTTGACTATATTCGTAAAGCCAAAGTGGCGGCAGGCGAAGCAGGCGGGATCACGCAACATATCGGTGCGTACCATGTGGAAACCGACGACGGCAAAATGATCACTTTCTTAGATACGCCAGGACATGCGGCGTTTACTTCCATGCGTGCGCGTGGTGCGAAAGCGACGGACATCGTGGTGC  >  1/1142014‑1142498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑