Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,135,069	T→C	intergenic (+19579/‑3985)	unknown → / → unknown	tRNA‑Gln‑TTG/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,135,069	0	T	C	100.0%	215.0 / NA	74	intergenic (+19579/‑3985)	unknown/unknown	tRNA‑Gln‑TTG/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (39/35);  total (39/35)

CTACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTTAGCATTTTTATTACGTCTATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACATACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGAATTGAAATGATATGAAATAGCAAAACCCCGACATAGCGGGGCTTTATGGCTATTTTGAAATGATATGAAGTGTTATGAAATGCAGGAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGAAATGCCGTTTACTTCATTGATAATGCGATTGGAAATTCTGCCGAGCAAATCATAAGGCAGGTGTGCCCAATGTGCGGTCATAAAATCGATCGTTTCTACGGCGCGCAGGGAGATAACCCAGTCATATTTACGCCCGTCGCCCATCA  >  1/1134836‑1135301                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                         cgaCTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGa                                                                                                                                                                                                                         <  1:115321/249‑1 (MQ=255) cgaCTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGa                                                                                                                                                                                                                         <  1:303613/249‑1 (MQ=255) cgaCTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGa                                                                                                                                                                                                                         <  1:739579/249‑1 (MQ=255)   aCTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGaaa                                                                                                                                                                                                                       >  1:498134/1‑251 (MQ=255)      ttATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGaaa                                                                                                                                                                                                                       <  1:326175/248‑1 (MQ=255)                         gTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTcc                                                                                                                                                                                                      <  1:894574/246‑1 (MQ=255)                                tgatttgattTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCg                                                                                                                                                                                            <  1:263553/249‑1 (MQ=255)                                  atttgatttTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGc                                                                                                                                                                                        <  1:279054/251‑1 (MQ=255)                                                       gCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGaaa                                                                                                                                                                                                                       >  1:814399/1‑199 (MQ=255)                                                       gCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGt                                                                                                                                                                    >  1:759583/1‑250 (MQ=255)                                                          tttttATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCata                                                                                                                                                                >  1:326503/1‑251 (MQ=255)                                                          tttttATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCata                                                                                                                                                                <  1:509370/251‑1 (MQ=255)                                                           ttttATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCatat                                                                                                                                                               <  1:898925/251‑1 (MQ=255)                                                             ttattaCGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATAc                                                                                                                                                             <  1:242452/251‑1 (MQ=255)                                                                      ccattatACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTcc                                                                                                                                                                                                      >  1:851380/3‑201 (MQ=255)                                                                      ccattatACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATAc                                                                                                                                                             >  1:337712/3‑242 (MQ=255)                                                                      ccattatACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGa                                                                                                                                                    >  1:38019/3‑251 (MQ=255)                                                                      ccattatACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGa                                                                                                                                                    >  1:93094/3‑251 (MQ=255)                                                                       cattatACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATAc                                                                                                                                                             >  1:19638/2‑241 (MQ=255)                                                                                gTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGAAATGCCGttt                                                                                                                                          >  1:77657/1‑251 (MQ=255)                                                                                 tCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGAAATGCCGTTTa                                                                                                                                         >  1:333187/1‑251 (MQ=255)                                                                                   tGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGAAATGCCGTTTACt                                                                                                                                       <  1:700889/251‑1 (MQ=255)                                                                                                 aTTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGAAATGCCGTTTACTTCATTGATAATGCg                                                                                                                         >  1:506800/1‑251 (MQ=255)                                                                                                    tttGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGAAATGCCGTTTAc                                                                                                                                        <  1:583874/233‑1 (MQ=255)                                                                                                          cTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAGATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGAAATGCCGTTTACTTCATTGATAATGCGATTGGAAAt                                                                                                                >  1:215371/1‑251 (MQ=255)                                                                                                                     gtgtTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAGCCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGcggaaata                                                                                                                                                                                                             >  1:718824/1‑140 (MQ=255)                                                                                                                     gtgtTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTc                                                                                                                                                                                                 >  1:372119/1‑159 (MQ=255)                                                                                                                     gtgtTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGAAATGCCGTTTACTTCATTGATAAt                                                                                                                            >  1:85610/1‑228 (MQ=255)                                                                                                                        tttCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACcac                                                                                                                                                          >  1:713069/1‑195 (MQ=255)                                                                                                                          tCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGAAATGCCGTTTACTTCATTGATAATGCGATTGGAAATTCTGCCGAGCAAATCa                                                                                                >  1:649806/1‑251 (MQ=255)                                                                                                                           cAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATAc                                                                                                                                                             >  1:694642/1‑189 (MQ=255)                                                                                                                             atacacacCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGa                                                                                                                                                                                                                         <  1:541859/127‑1 (MQ=255)                                                                                                                             atacacacCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATAc                                                                                                                                                             <  1:737307/187‑1 (MQ=255)                                                                                                                                cacacCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGAAATGCCGTTTACTTCATTGATAATGCGATTGGAAATTCTGCCGAGCAAATCATAAGGc                                                                                          <  1:269726/248‑1 (MQ=255)                                                                                                                                     cGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGAAATGCCGTTTACTTCATTGATAATGCGATTGGAAATTCTGCCGAGCAAATCATAAGGCAGgtg                                                                                     <  1:337328/251‑1 (MQ=255)                                                                                                                                              tAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGAAATGCCGTTTACTTCATTGATAATGCGATTGGAAATTCTGCCGAGCAAATCATAAGGCAGGTGTGCCCAAtg                                                                            <  1:796132/251‑1 (MQ=255)                                                                                                                                                                aTACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACg                                                                                                                                                       >  1:428089/1‑158 (MQ=255)                                                                                                                                                                aTACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGAAATGCCGTTTACTTCATTGATAATGCGATTGGAAATTCTGCCGAGCAAATCATAAGGc                                                                                          <  1:151823/219‑1 (MQ=255)                                                                                                                                                                   ctatgaaatggtatgaaacgccatGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGAAATGCCGTTTACTTCATTGATAATGCGATTGGAAATTCTGCCGAGCAAATCATAAGGCAGGTGTGccc                                                                                <  1:256910/204‑1 (MQ=255)                                                                                                                                                                   ctatgaaatggtatgaaacgccatGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGAAATGCCGTTTACTTCATTGATAATGCGATTGGAAATTCTGCCGAGCAAATCATAAGGCAGGTGTGCCCAATGTGCGGTCATAAAATCGATCGt                                                       >  1:496205/23‑251 (MQ=255)                                                                                                                                                                   ctatgaaatggtatgaaacgccatGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGAAATGCCGTTTACTTCATTGATAATGCGATTGGAAATTCTGCCGAGCAAATCATAAGGCAGGTGTGCCCAATGTGCGGTCATAAAATCGATCGt                                                       >  1:123122/23‑251 (MQ=255)                                                                                                                                                                       gaaatggtatgaaacgccatGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGAAATGCCGTTTACTTCATTGATAATGCGATTGGAAATTCTGCCGAGCAAATCATAAGGCAGgtg                                                                                     <  1:242988/199‑1 (MQ=255)                                                                                                                                                                        aaatggtatgaaacgccatGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGAAATGCCGTTTACTTCATTGATAATGCGATTGGAAATTCTGCCGAGCAAATCATAAGGCAGGTGTGCCCAATGTGCGGTCATAAAATCGATCGTTTCTa                                                  >  1:335040/18‑251 (MQ=255)                                                                                                                                                                           tggtatgaaacgccatGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGAAATGCCGTTTACTTCATTGATAATGCGATTGGAAATTCTGCCGAGCAAATCATAAGGCAGGTGTGCCCAATGTGCGGTCATAAAATCGATCGTTTCTACgg                                               <  1:268933/237‑1 (MQ=255)                                                                                                                                                                           tggtatgaaacgccatGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGAAATGCCGTTTACTTCATTGATAATGCGATTGGAAATTCTGCCGAGCAAATCATAAGGCAGGTGTGCCCAATGTGCGGTCATAAAATCGATCGTTTCTACgg                                               <  1:869657/237‑1 (MQ=255)                                                                                                                                                                           tggtatgaaacgccatGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGAAATGCCGTTTACTTCATTGATAATGCGATTGGAAATTCTGCCGAGCAAATCATAAGGCAGGTGTGCCCAATGTGCGGTCATAAAATCGATCGTTTCTACgg                                               <  1:846966/237‑1 (MQ=255)                                                                                                                                                                            ggtatgaaacgccatGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATAc                                                                                                                                                             >  1:717609/14‑140 (MQ=255)                                                                                                                                                                             gtatgaaacgccatGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGAAATGCCGTTTACTTCATTGa                                                                                                                                <  1:80195/156‑1 (MQ=255)                                                                                                                                                                             gtatgaaacgccatGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGAAATGCCGTTTACTTCATTGATAATGCGATTGGAAATTCTGCCGAGCAAATCATAAGGCAGGTGTGCCCAATGTGCGGTCATAAAATCGATCGTTTCTACGgcg                                             >  1:274762/13‑251 (MQ=255)                                                                                                                                                                             gtatgaaacgccatGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGAAATGCCGTTTACTTCATTGATAATGCGATTGGAAATTCTGCCGAGCAAATCATAAGGCAGGTGTGCCCAATGTGCGGTCATAAAATCGATCGTTTCTACGgcg                                             >  1:88981/13‑251 (MQ=255)                                                                                                                                                                             gtatgaaacgccatGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGAAATGCCGTTTACTTCATTGATAATGCGATTGGAAATTCTGCCGAGCAAATCATAAGGCAGGTGTGCCCAATGTGCGGTCATAAAATCGATCGTTTCTACGgcg                                             >  1:898333/13‑251 (MQ=255)                                                                                                                                                                               atgaaacgccatGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGAAATGCCGTTTACTTCATTGATAATGCGATTGGAAATTCTGCCGAGCAAATCATAAGGCAGGTGTGCCCAATGTGCGGTCATAAAATCGATCGTTTCTACGgcgcg                                           >  1:720754/11‑251 (MQ=255)                                                                                                                                                                                           gAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGAAATGCCGTTTACTTCATTGATAATGCGATTGGAAATTCTGCCGAGCAAATCATAAGGc                                                                                          >  1:21730/1‑192 (MQ=255)                                                                                                                                                                                            aaaTAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGAAATGCCGTTTACTTCATTGATAATGCGATTGGAAATTCTGCCGAGCAAATCATAAGGCAGGTGTGCCCAATGTGCGGTCATAAAATCGATCGTTTCTACGGCGCGCAGGGAGATAAcc                              <  1:860458/251‑1 (MQ=255)                                                                                                                                                                                            aaaTAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGAAATGCCGTTTACTTCATTGATAATGCGATTGGAAATTCTGCCGAGCAAATCATAAGGCAGGTGTGCCCAATGTGCGGTCATAAAATCGATCGTTTCTACGGCGCGCAGGGAGATAAc                               <  1:619546/250‑1 (MQ=255)                                                                                                                                                                                               taacAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGAAATGCCGTTTACTTCATTGATAATGCGATTGGAAATTCTGCCGAGCAAATCATAAGGCAGGTGTGCCCAAt                                                                             >  1:796768/4‑201 (MQ=255)                                                                                                                                                                                                      aCCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGAAATGCCGTTTACTTCATTGATAATGCGATTGGAAATTCTGCCGAGCAAATCATAAGGc                                                                                          <  1:558168/181‑1 (MQ=255)                                                                                                                                                                                                       ccccGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGAAATGCCGTTTACTTCATTGATAATGCGATTGGAAATTCTGCCGAGCAAATCATAAGGCAGGTGTGCCCAATGTGCGGTCATAAAATCGATCGTTTCTACGGCGCGCAGGGAGATAACCCAGTCATAttt                   <  1:101906/251‑1 (MQ=255)                                                                                                                                                                                                       ccccGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGAAATGCCGTTTACTTCATTGATAATGCGATTGGAAATTCTGCCGAGCAAATCATAAGGCAGGTGTGCCCAATGTGCGGTCATAAAATCGATCGTTTCTACGGCGCGCAGGGAGATAACCCAGTCATAttt                   <  1:669133/251‑1 (MQ=255)                                                                                                                                                                                                            aCATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGAAATGCCGTTTACTTCATTGATAATGCGATTGGAAATTCTGCCGAGCAAATCATAAGGCAGGTGTGCCCAATGTGCGGTCATAAAatcgatcg                                                        <  1:574752/209‑1 (MQ=255)                                                                                                                                                                                                            aCATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGAAATGCCGTTTACTTCATTGATAATGCGATTGGAAATTCTGCCGAGCAAATCATAAGGCAGGTGTGCCCAATGTGCGGTCATAAAATCGATCGTTTCTACGGCGCGCAGGGAGATAACCCAGTCATATTTACGc               <  1:20306/250‑1 (MQ=255)                                                                                                                                                                                                            aCATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGAAATGCCGTTTACTTCATTGATAATGCGATTGGAAATTCTGCCGAGCAAATCATAAGGCAGGTGTGCCCAATGTGCGGTCATAAAATCGATCGTTTCTACGGCGCGCAGGGAGATAACCCAGTCATATTTACGc               <  1:185804/250‑1 (MQ=255)                                                                                                                                                                                                               tagaggggCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGAAATGCCGTTTACTTCATTGATAATGCGATTGGAAATTCTGc                                                                                                           >  1:716219/5‑155 (MQ=255)                                                                                                                                                                                                               tagaggggCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGAAATGCCGTTTACTTCATTGATAATGCGATTGGAAATTCTGCCGAGCAAATCATAAGGCAGGTGTGCCCAATGTGCGGTCATAAAATCGATCGTTTCTACGGCGCGCAGGGAGATAAccc                             <  1:715176/229‑1 (MQ=255)                                                                                                                                                                                                                    gggCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGAAATGCCGTTTACTTCATTGATAATGCGATTGGAAATTCTGCCGAGCAAATCATAAGGCAGGTGTGCCCAATGTGCGGTCATAAAATCGATCGTTTCTACGGCGCGCAGGGAGATAACCCAGTCATATTTACGCCCGTCGcc       >  1:488196/1‑250 (MQ=255)                                                                                                                                                                                                                      gCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGAAATGCCGTTTACTTCATTGATAATGCGATTGGAAATTCTGCCGAGCAAATCATAAGGCAGGTGTGCCCAATGTGCGGTCATAAAATCGATCGTTTCTACGGCGCGCAGGGAGATAACCCAGTCATATTTACGCCCGTCGCCCAt    >  1:632577/1‑251 (MQ=255)                                                                                                                                                                                                                      gCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGAAATGCCGTTTACTTCATTGATAATGCGATTGGAAATTCTGCCGAGCAAATCATAAGGCAGGTGTGCCCAATGTGCGGTCATAAAATCGATCGTTTCTACGGCGCGCAGGGAGATAACCCAGTCATATTTACGCCCGTCGCCCAt    >  1:310514/1‑251 (MQ=255)                                                                                                                                                                                                                      gCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGAAATGCCGTTTACTTCATTGATAATGCGATTGGAAATTCTGCCGAGCAAATCATAAGGCAGGTGTGCCCAATGTGCGGTCATAAAATCGATCGTTTCTACGGCGCGCAGGGAGATAACCCAGTCATATTTACGCCCGTCGCCCAt    >  1:732216/1‑251 (MQ=255)                                                                                                                                                                                                                      gCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGAAATGCCGTTTACTTCATTGATAATGCGATTGGAAATTCTGCCGAGCAAATCATAAGGCAGGTGTGCCCAATGTGCGGTCATAAAATCGATCGTTTCTACGGCGCGCAGGGAGATAACCCAGTCATATTTACGCCCGTCGCCCAt    >  1:42772/1‑251 (MQ=255)                                                                                                                                                                                                                      gCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGAAATGCCGTTTACTTCATTGATAATGCGATTGGAAATTCTGCCGAGCAAATCATAAGGCAGGTGTGCCCAATGTGCGGTCATAAAATCGATCGTTTCTACGGCGCGCAGGGAGA‑AACCCAGTCATATTTACGCCCGTCGCCCAt    >  1:172594/1‑250 (MQ=255)                                                                                                                                                                                                                       ctttataacTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGAAATGc                                                                                                                                               <  1:402985/103‑1 (MQ=255)                                                                                                                                                                                                                       ctttataacTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGAAATGCCGTTTACTTCATTGATAATGCGATTGGAAATTCTGCCGAGCAAATCATAAGGCAGGTGTGCCCAATGTGCGGTCATAAAATCGATCGTTTCTACGGCGCGCAGGGAGATAACCCAGTCATATTTACGCCCGTCGCCCAt    >  1:93873/9‑250 (MQ=255)                                                                                                                                                                                                                         ttataacTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGAAATGCCGTTTACTTCATTGATAATGCGATTGGAAATTCTGCCGAGCAAATCATAAGGCAGGTGTGCCCAATGTGCGGTCATAAAATCGATCGTTTCTACGGCGCGCAGGGAGATAACCCAGTCa                        <  1:150084/222‑1 (MQ=255)                                                                                                                                                                                                                         ttataacTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGAAATGCCGTTTACTTCATTGATAATGCGATTGGAAATTCTGCCGAGCAAATCATAAGGCAGGTGTGCCCAATGTGCGGTCATAAAATCGATCGTTTCTACGGCGCGCAGGGAGATAACCCAGTCATATTTACGCCCGTCGCCCATca  >  1:599265/7‑250 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                         CTACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTTAGCATTTTTATTACGTCTATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACATACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGAATTGAAATGATATGAAATAGCAAAACCCCGACATAGCGGGGCTTTATGGCTATTTTGAAATGATATGAAGTGTTATGAAATGCAGGAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGAAATGCCGTTTACTTCATTGATAATGCGATTGGAAATTCTGCCGAGCAAATCATAAGGCAGGTGTGCCCAATGTGCGGTCATAAAATCGATCGTTTCTACGGCGCGCAGGGAGATAACCCAGTCATATTTACGCCCGTCGCCCATCA  >  1/1134836‑1135301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑