Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,135,016	T→C	intergenic (+19526/‑4038)	unknown → / → unknown	tRNA‑Gln‑TTG/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,135,016	0	T	C	100.0%	179.8 / NA	62	intergenic (+19526/‑4038)	unknown/unknown	tRNA‑Gln‑TTG/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/30);  total (32/30)

GTTATTGTTCCCGAGGGTGTTACGGATACATAAAGCCCATCACGATCGGCTACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTTAGCATTTTTATTACGTCTATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACATACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGAATTGAAATGATATGAAATAGCAAAACCCCGACATAGCGGGGCTTTATGGCTATTTTGAAATGATATGAAGTGTTATGAAATGCAGGAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGAAATGCCGTTTACTTCATTGATAATGCGATTGGAAATTCTGCCGAGCAAATCATAAGGCAGGTGTGCCCAATG  >  1/1134787‑1135227                                                                                                                                                                                                                                      |                                                                                                                                                                                                                    gtgatagtccctgcgggagtgactgATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAAccc                                                                                                                                                                                                 >  1:848490/25‑250 (MQ=255)       gtccctgcgggagtgactgATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGAc                                                                                                                                                                                             >  1:312119/19‑248 (MQ=255)       gtccctgcgggagtgactgATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATa                                                                                                                                                                                          >  1:882325/19‑251 (MQ=255)        tccctgcgggagtgactgATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACACCGACAt                                                                                                                                                                                           <  1:301516/232‑1 (MQ=255)          cctgcgggagtgactgATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAgag                                                                                                                                                                                       <  1:86361/236‑3 (MQ=255)           ctgcgggagtgactgATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAgagg                                                                                                                                                                                      <  1:567246/237‑4 (MQ=255)           ctgcgggagtgactgATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAgagg                                                                                                                                                                                      <  1:521015/237‑4 (MQ=255)                     gactgATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAAcccc                                                                                                                                                                                                <  1:729126/227‑1 (MQ=255)                     gactgATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTAtaa                                                                                                                                                                            >  1:632161/5‑249 (MQ=255)                      actgATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTAtaac                                                                                                                                                                           <  1:700471/248‑4 (MQ=255)                      actgATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTAtaac                                                                                                                                                                           <  1:485276/248‑4 (MQ=255)                       ctgattcATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAgagg                                                                                                                                                                                      <  1:713484/233‑4 (MQ=255)                          aTACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTAtaactatt                                                                                                                                                                       >  1:775076/1‑244 (MQ=255)                             cATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAGTAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTg                                                                                                                                                                    >  1:447236/1‑251 (MQ=255)                             cATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTg                                                                                                                                                                    >  1:876352/1‑251 (MQ=255)                                       gtCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGaaac                                                                                                                                                                <  1:576753/244‑2 (MQ=255)                                                  cgaCTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGa                                                                                                                                               <  1:115321/249‑1 (MQ=255)                                                  cgaCTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGa                                                                                                                                               <  1:303613/249‑1 (MQ=255)                                                  cgaCTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGa                                                                                                                                               <  1:739579/249‑1 (MQ=255)                                                    aCTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTAtaac                                                                                                                                                                           >  1:36541/1‑218 (MQ=255)                                                    aCTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGa                                                                                                                                                                   >  1:562477/1‑229 (MQ=255)                                                    aCTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGaaa                                                                                                                                             >  1:498134/1‑251 (MQ=255)                                                       ttATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGaaa                                                                                                                                             <  1:326175/248‑1 (MQ=255)                                                                         ggTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATACCAAAACCCCGACATAGAGGGGCTTTAtaac                                                                                                                                                                           >  1:424117/1‑197 (MQ=255)                                                                          gTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTcc                                                                                                                            <  1:894574/246‑1 (MQ=255)                                                                                 tgatttgattTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCg                                                                                                                  <  1:263553/249‑1 (MQ=255)                                                                                   atttgatttTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGc                                                                                                              <  1:279054/251‑1 (MQ=255)                                                                                                        gCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGaaa                                                                                                                                             >  1:814399/1‑199 (MQ=255)                                                                                                        gCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGt                                                                                          >  1:759583/1‑250 (MQ=255)                                                                                                           tttttATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCata                                                                                      <  1:509370/251‑1 (MQ=255)                                                                                                           tttttATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCata                                                                                      >  1:326503/1‑251 (MQ=255)                                                                                                            ttttATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCatat                                                                                     <  1:898925/251‑1 (MQ=255)                                                                                                              ttattaCGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATAc                                                                                   <  1:242452/251‑1 (MQ=255)                                                                                                                       ccattatACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTcc                                                                                                                            >  1:851380/3‑201 (MQ=255)                                                                                                                       ccattatACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATAc                                                                                   >  1:337712/3‑242 (MQ=255)                                                                                                                       ccattatACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGa                                                                          >  1:93094/3‑251 (MQ=255)                                                                                                                       ccattatACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGa                                                                          >  1:38019/3‑251 (MQ=255)                                                                                                                       ccattatACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGAATGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAgagg                                                                                                                                                                                      >  1:790231/3‑140 (MQ=255)                                                                                                                        cattatACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATAc                                                                                   >  1:19638/2‑241 (MQ=255)                                                                                                                                 gTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGAAATGCCGttt                                                                >  1:77657/1‑251 (MQ=255)                                                                                                                                  tCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGAAATGCCGTTTa                                                               >  1:333187/1‑251 (MQ=255)                                                                                                                                    tGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGAAATGCCGTTTACt                                                             <  1:700889/251‑1 (MQ=255)                                                                                                                                                gTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGaaacgtcatgaaa                                                                                                                                                       <  1:548186/149‑11 (MQ=255)                                                                                                                                                  aTTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGAAATGCCGTTTACTTCATTGATAATGCg                                               >  1:506800/1‑251 (MQ=255)                                                                                                                                                     tttGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGAAATGCCGTTTAc                                                              <  1:583874/233‑1 (MQ=255)                                                                                                                                                           cTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAGATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGAAATGCCGTTTACTTCATTGATAATGCGATTGGAAAt                                      >  1:215371/1‑251 (MQ=255)                                                                                                                                                                      gtgtTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAGCCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGcggaaata                                                                                                                                   >  1:718824/1‑140 (MQ=255)                                                                                                                                                                      gtgtTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTc                                                                                                                       >  1:372119/1‑159 (MQ=255)                                                                                                                                                                      gtgtTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGAAATGCCGTTTACTTCATTGATAAt                                                  >  1:85610/1‑228 (MQ=255)                                                                                                                                                                         tttCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACcac                                                                                >  1:713069/1‑195 (MQ=255)                                                                                                                                                                           tCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGaaacgtcatgaaa                                                                                                                                                       <  1:63604/122‑11 (MQ=255)                                                                                                                                                                           tCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGAAATGCCGTTTACTTCATTGATAATGCGATTGGAAATTCTGCCGAGCAAATCa                      >  1:649806/1‑251 (MQ=255)                                                                                                                                                                            cAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATAc                                                                                   >  1:694642/1‑189 (MQ=255)                                                                                                                                                                             aaTACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTAt                                                                                                                                                                              <  1:151228/97‑1 (MQ=255)                                                                                                                                                                              atacacacCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGaaa                                                                                                                                                                 >  1:804624/1‑109 (MQ=255)                                                                                                                                                                              atacacacCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGa                                                                                                                                               <  1:541859/127‑1 (MQ=255)                                                                                                                                                                              atacacacCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATAc                                                                                   <  1:737307/187‑1 (MQ=255)                                                                                                                                                                                 cacacCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGAAATGCCGTTTACTTCATTGATAATGCGATTGGAAATTCTGCCGAGCAAATCATAAGGc                <  1:269726/248‑1 (MQ=255)                                                                                                                                                                                      cGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGAAATGCCGTTTACTTCATTGATAATGCGATTGGAAATTCTGCCGAGCAAATCATAAGGCAGgtg           <  1:337328/251‑1 (MQ=255)                                                                                                                                                                                               tAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGAAATGCCGTTTACTTCATTGATAATGCGATTGGAAATTCTGCCGAGCAAATCATAAGGCAGGTGTGCCCAAtg  <  1:796132/251‑1 (MQ=255)                                                                                                                                                                                                                 aTACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACg                                                                             >  1:428089/1‑158 (MQ=255)                                                                                                                                                                                                                 aTACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGAAATGCCGTTTACTTCATTGATAATGCGATTGGAAATTCTGCCGAGCAAATCATAAGGc                <  1:151823/219‑1 (MQ=255)                                                                                                                                                                                                                                      |                                                                                                                                                                                                                    GTTATTGTTCCCGAGGGTGTTACGGATACATAAAGCCCATCACGATCGGCTACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTTAGCATTTTTATTACGTCTATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACATACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGAATTGAAATGATATGAAATAGCAAAACCCCGACATAGCGGGGCTTTATGGCTATTTTGAAATGATATGAAGTGTTATGAAATGCAGGAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATACCACACGGGAAATGCCGTTTACTTCATTGATAATGCGATTGGAAATTCTGCCGAGCAAATCATAAGGCAGGTGTGCCCAATG  >  1/1134787‑1135227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑