Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,134,906	T→C	intergenic (+19416/‑4148)	unknown → / → unknown	tRNA‑Gln‑TTG/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,134,906	0	T	C	100.0%	154.2 / NA	52	intergenic (+19416/‑4148)	unknown/unknown	tRNA‑Gln‑TTG/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/30);  total (22/30)

AATGGTTAGTCGTAGCGGAATGTTATTGTTCCCGAGGGTGTTACGGATACATAAAGCCCATCACGATCGGCTACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTTAGCATTTTTATTACGTCTATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACATACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGAATTGAAATGATATGAAATAGCAAAACCCCGACATAGCGGGGCTTTATGGCTATTTTGAAATGATATGAAGTGTTATGAAATGCAGGAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATAC  >  1/1134766‑1135146                                                                                                                                             |                                                                                                                                                                                                                                                 attctgtAGTCGTAACGGAATGTGATAGTCCCTGCGGGAGTGACTGATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTatgaaatggtatgaaac                                                                                                                                    <  1:866294/245‑10 (MQ=255) attctgtAGTCGTAACGGAATGTGATAGTCCCTGCGGGAGTGACTGATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTatgaaatggtatgaaa                                                                                                                                     >  1:348179/7‑242 (MQ=255)    ctgtAGTCGTAACGGAATGTGATAGTCCCTGCGGGAGTGACTGATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATAc                                                                                                                                                                                                                                           <  1:255045/142‑1 (MQ=255)         gTCGTAACGGAATGTGATAGTCCCTGCGGGAGTGACTGATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAg                                                                                                                                                                                                                                <  1:152156/151‑1 (MQ=255)         gTCGTAACGGAATGTGATAGTCCCTGCGGGAGTGACTGATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCaa                                                                                                                                                                                            >  1:32497/1‑187 (MQ=255)                      gtgatagtccctgcgggagtgactgATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAAccc                                                                                                                >  1:848490/25‑250 (MQ=255)                            gtccctgcgggagtgactgATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGAc                                                                                                            >  1:312119/19‑248 (MQ=255)                            gtccctgcgggagtgactgATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATa                                                                                                         >  1:882325/19‑251 (MQ=255)                             tccctgcgggagtgactgATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACACCGACAt                                                                                                          <  1:301516/232‑1 (MQ=255)                               cctgcgggagtgactgATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAgag                                                                                                      <  1:86361/236‑3 (MQ=255)                                ctgcgggagtgactgATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAgagg                                                                                                     <  1:521015/237‑4 (MQ=255)                                ctgcgggagtgactgATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAgagg                                                                                                     <  1:567246/237‑4 (MQ=255)                                          gactgATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTa                                                                                                                                                                                                                        >  1:141019/5‑126 (MQ=255)                                          gactgATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATAc                                                                                                                                                      <  1:468338/188‑1 (MQ=255)                                          gactgATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTatgaaatggtatgaaac                                                                                                                                    <  1:455610/206‑10 (MQ=255)                                          gactgATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAAcccc                                                                                                               <  1:729126/227‑1 (MQ=255)                                          gactgATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTAtaa                                                                                           >  1:632161/5‑249 (MQ=255)                                           actgATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTAtaac                                                                                          <  1:700471/248‑4 (MQ=255)                                           actgATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTAtaac                                                                                          <  1:485276/248‑4 (MQ=255)                                            ctgattcATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAgagg                                                                                                     <  1:713484/233‑4 (MQ=255)                                              gATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATAc                                                                                                                                                      >  1:165059/1‑188 (MQ=255)                                               aTACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTAtaactatt                                                                                      >  1:775076/1‑244 (MQ=255)                                                 aCATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTatga                                                                                                                                                 <  1:421774/190‑1 (MQ=255)                                                  cATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATAc                                                                                                                                                                                                                                           <  1:539925/99‑1 (MQ=255)                                                  cATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAGTAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTg                                                                                   >  1:447236/1‑251 (MQ=255)                                                  cATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTg                                                                                   >  1:876352/1‑251 (MQ=255)                                                  cATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGAATTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTatgaaat                                                                                                                                              >  1:93961/1‑192 (MQ=255)                                                            gtCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGaaac                                                                               <  1:576753/244‑2 (MQ=255)                                                                  atcagcgaCTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTatgaaa                                                                                                                                               <  1:243842/168‑1 (MQ=255)                                                                       cgaCTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGa                                                              <  1:739579/249‑1 (MQ=255)                                                                       cgaCTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGa                                                              <  1:115321/249‑1 (MQ=255)                                                                       cgaCTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGa                                                              <  1:303613/249‑1 (MQ=255)                                                                         aCTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGAtt                                                                                                                                                                                                     <  1:121858/114‑1 (MQ=255)                                                                         aCTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGAt                                                                                                                                                                                                      >  1:403280/1‑113 (MQ=255)                                                                         aCTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATaaaaa                                                                                                                                                              <  1:791556/153‑1 (MQ=255)                                                                         aCTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTatgaaatggtatga                                                                                                                                       >  1:234778/1‑170 (MQ=255)                                                                         aCTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTAtaac                                                                                          >  1:36541/1‑218 (MQ=255)                                                                         aCTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGa                                                                                  >  1:562477/1‑229 (MQ=255)                                                                         aCTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGaaa                                                            >  1:498134/1‑251 (MQ=255)                                                                            ttATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGaaa                                                            <  1:326175/248‑1 (MQ=255)                                                                                              ggTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATACCAAAACCCCGACATAGAGGGGCTTTAtaac                                                                                          >  1:424117/1‑197 (MQ=255)                                                                                               gTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTcc                                           <  1:894574/246‑1 (MQ=255)                                                                                                      tgatttgattTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCg                                 <  1:263553/249‑1 (MQ=255)                                                                                                        atttgatttTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGc                             <  1:279054/251‑1 (MQ=255)                                                                                                                             gCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAAt                                                                                                                                                        <  1:320159/107‑1 (MQ=255)                                                                                                                             gCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGaaa                                                            >  1:814399/1‑199 (MQ=255)                                                                                                                             gCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGt         >  1:759583/1‑250 (MQ=255)                                                                                                                                tttttATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTatgaaat                                                                                                                                              >  1:139649/1‑114 (MQ=255)                                                                                                                                tttttATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCata     <  1:509370/251‑1 (MQ=255)                                                                                                                                tttttATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCata     >  1:326503/1‑251 (MQ=255)                                                                                                                                 ttttATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCatat    <  1:898925/251‑1 (MQ=255)                                                                                                                                   ttattaCGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGAAACGTCATGAAATGTTATGAAATGCGGAAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATAc  <  1:242452/251‑1 (MQ=255)                                                                                                                                             |                                                                                                                                                                                                                                                 AATGGTTAGTCGTAGCGGAATGTTATTGTTCCCGAGGGTGTTACGGATACATAAAGCCCATCACGATCGGCTACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTTAGCATTTTTATTACGTCTATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACATACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGAATTGAAATGATATGAAATAGCAAAACCCCGACATAGCGGGGCTTTATGGCTATTTTGAAATGATATGAAGTGTTATGAAATGCAGGAATATTATTCCCACTCAATCGTTGCTGGTGGTTTTCCGCTGATGTCATATAC  >  1/1134766‑1135146

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑