Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,134,837	T→G	intergenic (+19347/‑4217)	unknown → / → unknown	tRNA‑Gln‑TTG/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,134,837	0	T	G	100.0%	83.1 / NA	30	intergenic (+19347/‑4217)	unknown/unknown	tRNA‑Gln‑TTG/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (13/17);  total (13/17)

GAATGGTTAGTCGTAGCGGAATGTTATTGTTCCCGAGGGTGTTACGGATACATAAAGCCCATCACGATCGGCTACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTTAGCATTTTTATTACGTCTATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACATACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGAATTGAAATGATATGAAATAGCAAAACCCCGACATAGCGGGGCTTTATGGCTATTTTGAAAT  >  1/1134765‑1135069                                                                         |                                                                                                                                                                                                                                         gattctgtAGTCGTAACGGAATGTGATAGTCCCTGCGGGAGTGACTGATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTttatta                                                                                                                                                                          >  1:530028/8‑137 (MQ=255)  attctgtAGTCGTAACGGAATGTGATAGTCCCTGCGGGAGTGACTGATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTatgaaatggtatgaaac                                                       <  1:866294/245‑10 (MQ=255)  attctgtAGTCGTAACGGAATGTGATAGTCCCTGCGGGAGTGACTGATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTatgaaatggtatgaaa                                                        >  1:348179/7‑242 (MQ=255)     ctgtAGTCGTAACGGAATGTGATAGTCCCTGCGGGAGTGACTGATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATAc                                                                                                                                                              <  1:255045/142‑1 (MQ=255)          gTCGTAACGGAATGTGATAGTCCCTGCGGGAGTGACTGATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAg                                                                                                                                                   <  1:152156/151‑1 (MQ=255)          gTCGTAACGGAATGTGATAGTCCCTGCGGGAGTGACTGATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCaa                                                                                                               >  1:32497/1‑187 (MQ=255)                       gtgatagtccctgcgggagtgactgATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAAccc                                   >  1:848490/25‑250 (MQ=255)                             gtccctgcgggagtgactgATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGAc                               >  1:312119/19‑248 (MQ=255)                             gtccctgcgggagtgactgATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATa                            >  1:882325/19‑251 (MQ=255)                              tccctgcgggagtgactgATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACACCGACAt                             <  1:301516/232‑1 (MQ=255)                                cctgcgggagtgactgATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAgag                         <  1:86361/236‑3 (MQ=255)                                 ctgcgggagtgactgATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAgagg                        <  1:521015/237‑4 (MQ=255)                                 ctgcgggagtgactgATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAgagg                        <  1:567246/237‑4 (MQ=255)                                           gactgATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTa                                                                                                                                           >  1:141019/5‑126 (MQ=255)                                           gactgATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATAc                                                                         <  1:468338/188‑1 (MQ=255)                                           gactgATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTatgaaatggtatgaaac                                                       <  1:455610/206‑10 (MQ=255)                                           gactgATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAAcccc                                  <  1:729126/227‑1 (MQ=255)                                           gactgATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTAtaa              >  1:632161/5‑249 (MQ=255)                                            actgATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTAtaac             <  1:485276/248‑4 (MQ=255)                                            actgATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTAtaac             <  1:700471/248‑4 (MQ=255)                                             ctgattcATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAgagg                        <  1:713484/233‑4 (MQ=255)                                               gATACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATAc                                                                         >  1:165059/1‑188 (MQ=255)                                                aTACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTAtaactatt         >  1:775076/1‑244 (MQ=255)                                                 tACATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGtg                                                                                                                                                                                                           <  1:318317/56‑1 (MQ=255)                                                  aCATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTatga                                                                    <  1:421774/190‑1 (MQ=255)                                                   cATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATAc                                                                                                                                                              <  1:539925/99‑1 (MQ=255)                                                   cATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAGTAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTg      >  1:447236/1‑251 (MQ=255)                                                   cATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTg      >  1:876352/1‑251 (MQ=255)                                                   cATAAAGCCCGTCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGAATTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTatgaaat                                                                 >  1:93961/1‑192 (MQ=255)                                                             gtCACGATCAGCGACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTGAGCATTTTTATTACGTCCATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACACACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGGTATGAAACGCCATGAAATAACAAAACCCCGACATAGAGGGGCTTTATAACTATTTTGaaac  <  1:576753/244‑2 (MQ=255)                                                                         |                                                                                                                                                                                                                                         GAATGGTTAGTCGTAGCGGAATGTTATTGTTCCCGAGGGTGTTACGGATACATAAAGCCCATCACGATCGGCTACTTTATAGAGTTTATCTTTTGGTTTTAGTGATTTGATTTTTGAATCGGTTAGCATTTTTATTACGTCTATTATACCGTCTGAAAAGTGTCGGTATTTTTGATCTTTTTGGATTGTGTTTCAATACATACCGTCAAAAATAACGCAATAAAAATGAAATACTATGAAATGAATTGAAATGATATGAAATAGCAAAACCCCGACATAGCGGGGCTTTATGGCTATTTTGAAAT  >  1/1134765‑1135069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑