Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 1,131,879 C→T intergenic (+16389/‑7175) unknown → / → unknown tRNA‑Gln‑TTG/tRNA‑Met‑CAT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*11,131,8790CT100.0% 66.7 / NA 25intergenic (+16389/‑7175)unknown/unknowntRNA‑Gln‑TTG/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/16);  total (9/16)

AATGTACTAAATGTTCATATTTAATTGATTTTAAAAGAAATAAATTTTCAAAATTTTTTGATTAATTGCTGAATTTGTGACCTAGATCACAAAAAAAAGAGGGGGGAATTTTAAAAAGTGCGGTAGGAATTGCGGGCAAAAGAAAACCGCCCGAAGGCGGTTTGTCGGTATATAAACTGATTAAGATCCTACATCGCAAAAAATTCCTTGAGGTGGGTTAAATTATTGAAGCACCTCAATTAGTTTAGTGGCAGTTTCCTTTCCGCGAGGTAATAACATTGTTTTCTTTAGAATATAACTAGGAAACTTCTCTTTTAAGTAGATATTTTTAAACCATTTCCTAAATTCACCTAAGCTAGAGTCAGGATATGCATTAATTTGTTGTGGATTTGAACCTGATTGCAAAAATTCTTCAGGATAAAAATGTGAACATTT  >  1/1131648‑1132082
                                                                                                                                                                                                                                       |                                                                                                                                                                                                           
aaTGTATTAAATGTTCATATTTAATTGATTTTAAAAGAAATAAATTTTCAAAATTTTTTGATTAATTGCTGAATTTGTGACCTAGATCACAAAAAAAAGAGGGGGGAATTTTAAAAAGTGCGGTAGGAATTGCGGGCAAAAGAAAACCGCCCGAAGGCGGTTTGTCGGTATATAAACTGATTAAGATCCTACATCGCAAAAAATTCCTTGAGGTGGGTTAAATTATTGAAGTACCTCAATTAGTTTAGTgg                                                                                                                                                                                          <  1:350309/251‑1 (MQ=255)
   gtattAAATGTTCATATTTAATTGATTTTAAAAGAAATAAATTTTCAAAATTTTTTGATTAATTGCTGAATTTGTGACCTAGATCACAAAAAAAAGAGGGGGGAATTTTAAAAAGTGCGGTAGGAATTGCGGGCAAAAGAAAACCGCCCGAAGGCGGTTTGTCGGTATATAAACTGATTAAGATCCTACATCGCAAAAAATTCCTTGAGGTGGGTTAAATTATTGAAGTACCTCAATTAGTTTAGTGGCAg                                                                                                                                                                                       >  1:291983/5‑251 (MQ=255)
                           aTTTTAAAAGAAATAAATTTTCAAAATTTTTTGATTAATTGCTGAATTTGTGACCTAGATCACAAAAAAAAGAGGGGGGAATTTTAAAAAGTGCGGTAGGAATTGCGGGCAAAAGAAAACCGCCCGAAGGCGGTTTGTCGGTATATAAACTGATTAAGATCCTACATCGCAAAAAATTCCTTGAGGTGGGTTAAATTATTGAAGTACCTCAATTAGTTTAGTGGCAGTTTCCTTTCCGCGAGGTAATAACa                                                                                                                                                               <  1:145024/251‑1 (MQ=255)
                                                cAAAATTTTTTGATTAATTGCTGAATTTGTGACCTAGATCACAAAAAAAAGAGGGGGGAATTTTAAAAAGTGCGGTAGGAATTGCGGGCAAAAGAAAACCGCCCGAAGGCGGTTTGTCGGTATATAAACTGATTAAGATCCTACATCGCAAAAAATTCCTTGAGGTGGGTTAAATTATTGAAGTACCTCAATTAGTTTAGTGGCAGTTTCCTTTCCGCGAGGTAATAACATTGTTTTCTTTAGAATATAAc                                                                                                                                          <  1:399670/251‑1 (MQ=255)
                                                                 ttGCTGAATTTGTGACCTAGATCACAAAAAAAAGAGGGGGGAATTTTAAAAAGTGCGGTAGGAATTGCGGGCAAAAGAAAACCGCCCGAAGGCGGTTTGTCGGTATATAAACTGATTAAGATCCTACATCGCAAAAAATTCCTTGAGGTGGGTTAAATTATTGAAGTACCTCAATTAGTTTAGTGGCAGTTTCCTTTCCGCGAGGTAATAACAt                                                                                                                                                              <  1:538589/214‑1 (MQ=255)
                                                                 ttGCTGAATTTGTGACCTAGATCACAAAAAAAAGAGGGGGGAATTTTAAAAAGTGCGGTAGGAATTGCGGGCAAAAGAAAACCGCCCGAAGGCGGTTTGTCGGTATATAAACTGATTAAGATCCTACATCGCAAAAAATTCCTTGAGGTGGGTTAAATTATTGAAGTACCTCAATTAGTTTAGTGGCAGTTTCCTTTCCGCGAGGTAATAACATTGTTTTCTTTAGAat                                                                                                                                               >  1:265624/1‑229 (MQ=255)
                                                                        aTTTGTGACCTAGATCACAAAAAAAAGAGGGGGGAATTTTAAAAAGTGCGGTAGGAATTGCGGGCAAAAGAAAACCGCCCGAAGGCGGTTTGTCGGTATATAAACTGATTAAGATCCTACATCGCAAAAAATTCCTTGAGGTGGGTTAAATTATTGAAGTACCTCAATTAGTTTAGTGGCAGTCTCCTTTCCGCGAGGTAATAACATTGTTTTCTTTAGAATATAACTa                                                                                                                                        <  1:286851/229‑1 (MQ=255)
                                                                                 cTAGATCACAAAAAAAAGAGGGGGGAATTTTAAAAAGTGCGGTAGGAATTGCGGGCAAAAGAAAACCGCCCGAAGGCGGTTTGTCGGTATATAAACTGATTAAGATCCTACATCGCAAAAAATTCCTTGAGGTGGGTTAAATTATTGAAGTACCTCAAttagtt                                                                                                                                                                                                >  1:86749/1‑164 (MQ=255)
                                                                                   aGATCACAAAAAAAAGAGGGGGGAATTTTAAAAAGTGCGGTAGGAATTGCGGGCAAAAGAAAACCGCCCGAAGGCGGTTTGTCGGTATATAAACTGATTAAGATCCTACATCGCAAAAAATTCCTTGAGGTGGGTTAAATTATTGAAGTACCTCAATTAGTTTAGTGGCAGTTTCCTTTCCGCGAGGTAATAACATTGTTTTCTTTAGAATATAAc                                                                                                                                          <  1:140500/216‑1 (MQ=255)
                                                                                             aaaaaGAGGGGGGAATTTTAAAAAGTGCGGTAGGAATTGCGGGCAAAAGAAAACCGCCCGAAGGCGGTTTGTCGGTATATAAACTGATTAAGATCCTACATCGCAAAAAATTCCTTGAGGTGGGTTAAATTATTGAAGTACCTCAATTAGTTTAGTGGCAGTTTCCTTTCCGCGAGGTAATAACATTGTTTTCTTTAGAATATAACTAGGAAACTTCTCTTTTAAGTAGATATTTTTAAAc                                                                                                       <  1:882210/241‑1 (MQ=255)
                                                                                               aaaGAGGGGGGAATTTTAAAAAGTGCGGTAGGAATTGCGGGCAAAAGAAAACCGCCCGAAGGCGGTTTGTCGGTATATAAACTGATTAAGATCCTACATCGCAAAAAATTCCTTGAGGTGGGTTAAATTATTGAAGTACCTCAATTAGTTTAGTGGCAGTTTCCTTTCCGCGAGGTAATAACATTGTTTTCTTTAGAATATAACTAGGAAACTTCTCTTTTAAGTAGATATTTTTAAACCATTTCCTAAAt                                                                                           <  1:873516/251‑1 (MQ=255)
                                                                                                                                                            gCGGTTTGTCGGTATATAAACTGATTAAGATCCTACATCGCAAAAAATTCCTTGAGGTGGGTTAAATTATTGAAGTACCTCAATTAGTTTAGTGGCAGTTTCCTTTCCGCGAGGTAATAACATTGTTTTCTTTAGAATATAACTAGGAAACTTCTCTTTTAAGTAGATATTTTTAAACCATTTCCTAAATTCACCTAAGCTAGAGTCAGGATATGCATTAATTTGTTGTGGATTTGAACCTGATTGCaaa                               <  1:135644/250‑1 (MQ=255)
                                                                                                                                                                       gTATATAAACTGATTAAGATCCTACATCGCAAAAAATTCCTTGAGGTGGGTTAAATTATTGAAGTACCTCAATTAGTTTAGTGGCAGTTTCCTTTCCGCGAGGTAATAAc                                                                                                                                                                <  1:129688/110‑1 (MQ=255)
                                                                                                                                                                       gTATATAAACTGATTAAGATCCTACATCGCAAAAAATTCCTTGAGGTGGGTTAAATTATTGAAGTACCTCAATTAGTTTAGTGGCAGTTTCCTTTCCGCGAGGTAATAACAt                                                                                                                                                              >  1:326978/1‑112 (MQ=255)
                                                                                                                                                                       gTATATAAACTGATTAAGATCCTACATCGCAAAAAATTCCTTGAGGTGGGTTAAATTATTGAAGTACCTCAATTAGTTTAGTGGCAGTTTCCTTTCCGCGAGGTAATAACATTGTTTTCTTTAGAATATAACTAgg                                                                                                                                      <  1:117301/136‑1 (MQ=255)
                                                                                                                                                                       gTATATAAACTGATTAAGATCCTACATCGCAAAAAATTCCTTGAGGTGGGTTAAATTATTGAAGTACCTCAATTAGTTTAGTGGCAGTTTCCTTTCCGCGAGGTAATAACATTGTTTTCTTTAGAATATAACTAGGAAACTTCTCTTTTAAGTAGATATTTTTAAACCATTTCCTAAATTCACCTAAGc                                                                                 <  1:827236/189‑1 (MQ=255)
                                                                                                                                                                       gTATATAAACTGATTAAGATCCTACATCGCAAAAAATTCCTTGAGGTGGGTTAAATTATTGAAGTACCTCAATTAGTTTAGTGGCAGTTTCCTTTCCGCGAGGTAATAACATTGTTTTCTTTAGAATATAACTAGGAAACTTCTCTTTTAAGTAGATATTTTTAAACCATTTCCTAAATTCACCTAAGCTAGAGTCAGGATATGCATTAATTTGTTGTGGATTTGAACCTGATTGCAAAAATTCTTCAGGa                   >  1:186542/1‑251 (MQ=255)
                                                                                                                                                                                  gATTAAGATCCTACATCGCAAAAAATTCCTTGAGGTGGGTTAAATTATTGAAGTACCTCAATTAGTTTAGTGGCAGTTTCCTTTCCGCGAGGTAATAACa                                                                                                                                                               >  1:308016/1‑100 (MQ=255)
                                                                                                                                                                                  gATTAAGATCCTACATCGCAAAAAATTCCTTGAGGTGGGTTAAATTATTGAAGTACCTCAATTAGTTTAGTGGCAGTTTCCTTTCCGCGAGGTAATAACATTGTTTTCTTTAGAATATAACTAGGAAACTTCTCTTTTAAGTAGATATTTTTAAACCATTTCCTAAATTCACCTAAGCTAGAGTCAGGATATGCATTAATTTGTTGTGGATTTGAACCTGATTGCAAAAATTCTTCAGGATAAAAATGTGa        >  1:295589/1‑251 (MQ=255)
                                                                                                                                                                                       aGATCCTACATCGCAAAAAATTCCTTGAGGTGGGTTAAATTATTGAAGTACCTCAATTAGTTTAGTGGCAGTTTCCTTTCCGCGAGGTAATAACATTGTTTTCTTTAGAATATAACTAGGAAACTTCTCTTTTAAGt                                                                                                                     <  1:11667/137‑1 (MQ=255)
                                                                                                                                                                                        gATCCTACATCGCAAAAAATTCCTTGAGGTGGGTTAAATTATTGAAGTACCTCAATTAGTTTAGTGGCAGTTTCCTTTCCGCGAGGTAATAACATTGTTTTCTTTAGAATATAACTAGGAAACTTCTCTTTTAAGTAGATATTTTTAAACCATTTCCTAAATTCACCTAAGCTAGAGTCAGGATATGCATTAATTTGTTGTGGATTTGAACCTGATTGCAAAAATTCTTCAGGATAAAAATGTGAACAttt  >  1:19038/1‑251 (MQ=255)
                                                                                                                                                                                         aTCCTACATCGCAAAAAATTCCTTGAGGTGGGTTAAATTATTGAAGTACCTCAATTAGTTTAGTGGCAGTTTCCTTTCCGCGAGGTAATAACATTGTTTTCTTTAGAat                                                                                                                                               <  1:786794/109‑1 (MQ=255)
                                                                                                                                                                                                                  aGGTGGGTTAAATTATTGAAGTACCTCAATTAGTTTAGTGGCAGTTTCCTTTCCGCGAGGTAATAACATTGTTTTCTTTAGAATATAACTAGGAAACTTCTCTTTTAAGTAGATATTTTTAAACCATTTCCTAAATTCACCTAAGCTAGAGTCAGGATATGCATTAATTTGTTGTGGATTTGAAcc                                         <  1:557894/186‑1 (MQ=255)
                                                                                                                                                                                                                              ttattGAAGTACCTCAATTAGTTTAGTGGCAGTTTCCTTTCCGCGAGGTAATAACATTGTTTTCTTTAGAATATAACTAGGAAACTTCTCTTTTAAGt                                                                                                                     >  1:832624/1‑98 (MQ=255)
                                                                                                                                                                                                                              ttattGAAGTACCTCAATTAGTTTAGTGGCAGTTTCCTTTCCGCGAGGTAATAACATTGTTTTCTTTAGAATATAACTAGGAAACTTCTCTTTTAAGTAGATATTTTTAAAcc                                                                                                      <  1:68850/113‑1 (MQ=255)
                                                                                                                                                                                                                                       |                                                                                                                                                                                                           
AATGTACTAAATGTTCATATTTAATTGATTTTAAAAGAAATAAATTTTCAAAATTTTTTGATTAATTGCTGAATTTGTGACCTAGATCACAAAAAAAAGAGGGGGGAATTTTAAAAAGTGCGGTAGGAATTGCGGGCAAAAGAAAACCGCCCGAAGGCGGTTTGTCGGTATATAAACTGATTAAGATCCTACATCGCAAAAAATTCCTTGAGGTGGGTTAAATTATTGAAGCACCTCAATTAGTTTAGTGGCAGTTTCCTTTCCGCGAGGTAATAACATTGTTTTCTTTAGAATATAACTAGGAAACTTCTCTTTTAAGTAGATATTTTTAAACCATTTCCTAAATTCACCTAAGCTAGAGTCAGGATATGCATTAATTTGTTGTGGATTTGAACCTGATTGCAAAAATTCTTCAGGATAAAAATGTGAACATTT  >  1/1131648‑1132082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑