Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,129,676	G→A	intergenic (+14186/‑9378)	unknown → / → unknown	tRNA‑Gln‑TTG/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,129,676	0	G	A	100.0%	154.0 / NA	55	intergenic (+14186/‑9378)	unknown/unknown	tRNA‑Gln‑TTG/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/28);  total (27/28)

ATTTAAAAGATACTATTTTGTCCGTTGATACAGACAAAGCGTTTCTAATAATTTTCCCACAGTGGGAATCGCAGCTACTGGAAAGTTTGCCGCCCCGTAAAGCGGGACTACCTGCTACTCCCGCCGATGTATCTTATCTAGACAAATACAAGCCAAAGAAATAAACAACCCTAACCAACCCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGACAATAAGATGTTGAATGAACAAGAAAAACACGCGTTTTTAAATGGCAATTACGGTATTCCGTTAGTGGGACGGTATATGTCTTCTCCGGTTGCGGCAGGGCAAGTTGTTTCGGCTGTTGGGCTTCGCGGTCTAAAATATCCAGCACCCAGCGGCGGAAGTCTTTGGCTACTTTGGTGTGGCTTAACATGCCGATGAGGTGTGCGCCGCGCAAGGAGAAGATGCGCACTTTTTGGATTCCGCCATTTGT  >  1/1129447‑1129917                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  ttttAAAAGATACTATTTTGCCCGTTGATCCAGAAAAGGCGTTTTTAAAAATTTCCCCACAGGGGGAACCGCAGCTAGTGAAAAGTTTGCCGCCCCGAAAAGCGGGACTCCCTGCTACCCCCGCCGATGTATCGTATCTAGACAAATACAAGCCAAAGAAATAAACAACCCTAACCAACCCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGAAAATAAAATGTTGAATGAACAAGaaaaa                                                                                                                                                                                                                              <  1:106170/250‑1 (MQ=18) aTTTAAAAGATACTATTTTGTCCGTTGATACAGACAAAGCGTTTCTAATAATTTTCCCACAGTGGGAATCGCAGCTACTGGAAAGTTTGCCGCCCCGTAAAGCGGGACTACCTGCTACTCCCGCCGATGTATCGTATCTAGACAAATACAAGCCAAAGAAATAAACAACCCTAACCAACCCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGAAAATAAAATGTTGAATGAACAAGaaaaa                                                                                                                                                                                                                              >  1:812229/1‑251 (MQ=255)               aTTTTGTCCGTTGATACAGACAAAGCGTTTCTAATAATTTTCCCACAGTGGGAATCGCAGCTACTGGAAAGTTTGCCGCCCCGTAAAGCGGGACTACCTGCTACTCCCGCCGATGTATCGTATCTAGACAAATACAAGCCAAAGAAATAAACAACCCTAACCAACCCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGAAAATAAAATGTTGAATGAACAAGAAAAACACGCGTTTTTaaa                                                                                                                                                                                                                >  1:199340/1‑251 (MQ=255)               aTTTTGTCCGTTGATACAGACAAAGCGTTTCTAATAATTTTCCCACAGTGGGAATCGCAGCTACTGGAAAGTTTGCCGCCCCGTAAAGCGGGACTACCTGCTACTCCCGCCGATGTATCGTATCTAGACAAATACAAGCCAAAGAAATAAACAACCCTAACCAACCCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGAAAATAAAATGCTGAATGAACAAGAAAAACACGCGTTTTTaaa                                                                                                                                                                                                                >  1:299602/1‑251 (MQ=255)                       cGTTGATACAGACAAAGCGTTTCTAATAATTTTCCCACAGTGGGAATCGCAGCTACTGGAAAGTTTGCCGCCCCGTAAAGCGGGACTACCTGCTACTCCCGCCGATGTATCGTATCTAGACAAATACAAGCCAAAGAAATAAACAACCCTAACCAACCCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGAAAATAAAATGTTGAATGAACAAGAAAAACACGCGTTTTTAAATGGCAAtt                                                                                                                                                                                                        >  1:116227/1‑251 (MQ=255)                           gATACAGACAAAGCGTTTCTAATAATTTTCCCACAGTGGGAATCGCAGCTACTGGAAAGTTTGCCGCCCCGTAAAGCGGGACTACCTGCTACTCCCGCCGATGTATCGTATCTAGACAAATACAAGCCAAAGAAATAAACAACCCTAACCAACCCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGAAAATAAAATGTTGAATGAACAAGAAAAACACGCGTTTTTAAATGGCAATTACgg                                                                                                                                                                                                    >  1:404272/1‑251 (MQ=255)                           gATACAGACAAAGCGTTTCTAATAATTTTCCCACAGTGGGAATCGCAGCTACTGGAAAGTTTGCCGCCCCGTAAAGCGGGACTACCTGCTACTCCCGCCGATGTATCGTATCTAGACAAATACAAGCCAAAGAAATAAACAACCCTAACCAACCCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGAAAATAAAATGTTGAATGAACAAGAAAAACACGCGTTTTTAAATGGCAATTACgg                                                                                                                                                                                                    >  1:477918/1‑251 (MQ=255)                           gATACAGACAAAGCGTTTCTAATAATTTTCCCACAGTGGGAATCGCAGCTACTGGAAAGTTTGCCGCCCCGTAAAGCGGGACTACCTGCTACTCCCGCCGATGTATCGTATCTAGACAAATACAAGCCAAAGAAATAAACAACCCTAACCAACCCCAAGCCCTCCACGTGAGGGCCTTTTT‑TTACTCAAAAAAGGAAAATAAAATGTTGAATGAACAAGAAAAACACGCGTTTTTAAATGGCAATTACGGt                                                                                                                                                                                                   <  1:891697‑M2/251‑2 (MQ=255)                                         gTTTCTAATAATTTTCCCACAGTGGGAATCGCAGCTACTGGAAAGTTTGCCGCCCCGTAAAGCGGGACTACCTGCTACTCCCGCCGATGTATCGTATCTAGACAAATACAAGCCAAAGAAATAAACAACCCTAACCAACCCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGAAAATAAAATGTTGAATGAACAAGAAAAACACGCGTTTTTAAATGGCAATTACGGtatgacgcaatcgg                                                                                                                                                                                      <  1:327782‑M2/251‑15 (MQ=255)                                             cTAATAATTTCCCCACAGTGGAAATCGCAGCTACGGAAAAGTTTGCCGCCCCGTAAGGCGGGACTCCCTGCTACCCCCGCCGATGTATCGTATCTAGACAAATACAAGCCAAAGAAATAAACAACCCTAACCAACCCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGAAAATAAAATGTTGAATGAACAAGAAAAACACGCGTTTTTAAATGGCAATTACGGtatgacgcaatcggggga                                                                                                                                                                                  >  1:808589‑M2/1‑233 (MQ=255)                                                    ttttCCCACAGTGGGAATCGCAGCTACTGGAAAGTTTGCCGCCCCGTAAAGCGGGACTACCTGCTACTCCCGCCGATGTATCGTATCTAGACAAATACAAGCCAAAGAAATAAACAACCCTAACCAACCCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGAAAATAAAATGTTGAATGAACAAGAAAAACACGCGTTTTTAAATGGCAATTACGGtatc                                                                                                                                                                                                <  1:652294‑M2/230‑5 (MQ=255)                                                        cccACAGTGGGAATCGCAGCTACTGGAAAGTTTGCCGCCCCGTAAAGCGGGACTACCTGCTACTCCCGCCGATGTATCGTATCTAGACAAATACAAGCCAAAGAAATAAACAACCCTAACCAACCCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGAAAATAAAATGTtgaatga                                                                                                                                                                                                                                        >  1:581445/1‑186 (MQ=255)                                                        cccACAGGGGGAATCGCAGCTACGGAAAAGTTTGCCGCCCCGTAAAGGGGGACTACCTGCTACTCCCGCCGATGTATCGTATCTAGACAAATACAAGCCAAAGAAATAAACAACCCTAACCAACCCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGAAAATAAAATGTTGAATGAACAAGAAAAACACGCGTTTTTAAATGGCAATTACGGtatgacgcaatcgggggataagtgtaaac                                                                                                                                                                       >  1:852703‑M2/1‑222 (MQ=255)                                                              gggggaaCCGCAGCTACGGGAAAGTTTGCCGCCCCGAAAAGCGGAACTCCCTGCTACTCCCGCCGATGTATCGTATCTAGACAAATACAAGCCAAAGAAATAAACAACCCTAACCAACCCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGAAAATAAAATGTTGAATGAACAAGAAAAACACGCGTTTTTAAATGGCAATTACGGtatgacgcaatcgggggataag                                                                                                                                                                              >  1:367973‑M2/3‑216 (MQ=255)                                                                             aCTGGAAAGTTTGCCGCCCCGTAAAGCGGGACTACCTGCTACTCCCGCCGATGTATCGTATCTAGACAAATACAAGCCAAAGAAATAAACAACCCTAACCAACCCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGAAAATAAAATGTTGAATGAACAAGAAAAACACGCGTTTTTAAATGGCAATTACGGtatgacgcaatcgggggataagtgtaaactggtgttagtgggcgaaact                                                                                                                                                   <  1:557277‑M2/250‑50 (MQ=255)                                                                             aCTGGAAAGTTTGCCGCCCCGTAAAGCGGGACTACCTGCTACTCCCGCCGATGTATCGTATCTAGACAAATACAAGCCAAAGAAATAAACAACCCTAACCAACCCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGAAAATAAAATGTTGAATGAACAAGAAAAACACGCGTTTTTAAATGGCAATTACGGtatgacgcaatcgggggataagtgtaaactggtgttagtgggcgaaact                                                                                                                                                   <  1:857696‑M2/250‑50 (MQ=255)                                                                             aCTGGAAAGTTTGCCGCCCCGTAAAGCGGGACTACCTGCTACTCCCGCCGATGTATCGTATCTAGACAAATACAAGCCAAAGAAATAAACAACCCTAACCAACCCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGAAAATAAAATGTTGAATGAACAAGAAAAACACGCGTTTTTAAATGGCAATTACGGtatga                                                                                                                                                                                               <  1:415556‑M2/206‑6 (MQ=255)                                                                                                  aaaggcgggACTCCCTGCTACTCCCGCCGATGTATCGTATCTAGACAAATACAAGCCAAAGAAATAAACAACCCTAACCAACCCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGAAAATAAAATGTTGAATGAACAAGAAAAACACGCGTTTTTAAATGGCAATTACGGtatgacgcaatcgggggataagtgtaaactggtgttagtgggcgaaactcaccgagataatccgtatttgg                                                                                                                             >  1:701620‑M2/5‑180 (MQ=255)                                                                                                         ggACTACCTGCTACTCCCGCCGATGTATCGTATCTAGACAAATACAAGCCAAAGAAATAAACAACCCTAACCAACCCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGAAAATAAAATGTTGAATGAACAAGAAAAACACGCGTTTTTAAATGGCAATTGCGGtatgacgcaatcgggggataagtgtaaactggtgttagtgggcgaaactcaccgagataatccgtatttggttcttat                                                                                                                      <  1:445063‑M2/251‑79 (MQ=255)                                                                                                              aCCTGCTACTCCCGCCGATGTATCGTATCTAGACAAATACAAGCCAAAGAAATAAACAACCCTAACCAACCCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGAAAATAAAATGTTGAATGAACAAGAAAAACACGCGTTTTTAAATGGCAATTACGGtatgacgcaatcgggggataagtgtaaactggtgttagtgggcgaaactcaccgagataatccgtatttggttcttatcacca                                                                                                                 <  1:801944‑M2/251‑84 (MQ=255)                                                                                                              aCCTGCTACTCCCGCCGATGTATCGTATCTAGACAAATACAAGCCAAAGAAATAAACAACCCTAACCAACCCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGAAAATAAAATGTTGAATGAACAAGAAAAACACGCGTTTTTAAATGGCAATTACGGtatgacgcaatcgggggataagtgtaaactggtgttagtgggcgaaactcaccgagataatccgtatttggttcttatcacca                                                                                                                 <  1:669634‑M2/251‑84 (MQ=255)                                                                                                               ccTGCTCCTCCCGCCGATGTATCGTATCTAGACAAATACAAGCCAAAGAAATAAACAACCCTAACCAACCCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGAAAATAAAATGTTGAATGAACAAGAAAAACACGCGTTTTTAAATGGCAATTACGGtatgacgcaatcgggggataagtgtaaactggtgttagtgggcgaaactcaccgagataatccgtatttggttcttatcaccag                                                                                                                >  1:98062‑M2/1‑167 (MQ=255)                                                                                                                cTGCTACTCCCGCCGATGTATCGTATCTAGACAAATACAAGCCAAAGAAATAAACAACCCTAACCAACCCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGAAAATAAAATGTTGAATGAACAAGAAAAACACGCGTTTTTAAATGGCAATTACGGtatgacgcaatcgggggataagtgtaaactggtgttagtgggcgaaactcaccgagataatccgtatttggttcttatcaccaga                                                                                                               <  1:726462‑M2/251‑86 (MQ=255)                                                                                                                cTGCTACTCCCGCCGATGTATCGTATCTAGACAAATACAAGCCAAAGAAATAAACAACCCTAACCAACCCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGAAAATAAAATGTTGAATGAACAAGAAAAACACGCGTTTTTAAATGGCAATTACGGtatgacgcaatcgggggataagtgtaaactggtgttagtgggcgaaactcaccgagataatccgtatttggt                                                                                                                            <  1:792559‑M2/238‑73 (MQ=255)                                                                                                                     aCTCCCGCCGATGTATCGTATCTAGACAAATACAAGCCAAAGAAATAAACAACCCTAACCAACCCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGAAAATAAAATGTTGAATGAACAAGAAAAACACGCGTTTTTAAATGGCAATTACGGtatgacgcaatcgggggataagtgtaaactggtgttagtgggcgaaactcaccgagataatccgtatttggttcttatcaccagaggtga                                                                                                          <  1:682918‑M2/251‑91 (MQ=255)                                                                                                                         ccgccgATGTATCGTATCTAGACAAATACAAGCCAAAGAAATAAACAACCCTAACCAACCCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGAAAATAAAATGTTGAATGAACAAGAAAAACACGCGTTTTTAAATGGCAATTACGGtatgacgcaatcgggggataagtgtaaactggtgttagtgggcgaaactcaccgagataatccgtatttggttcttatcaccagaggtgacgat                                                                                                      >  1:400355‑M2/1‑157 (MQ=255)                                                                                                                            ccgATGTATCGTATCTAGACAAATACAAGCCAAAGAAATAAACAACCCTAACCAACCCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGAAAATAAAATGTTGAATGAACAAGAAAAACACGCGTTTTTAAATGGCAATTACGGtatgacgcaatcgggggataagtgtaaactggtgttagtgggcgaaactcaccgagataatccgtatttggttcttatcaccag                                                                                                                <  1:508912‑M2/238‑85 (MQ=255)                                                                                                                                          ctAGACAAATACAAGCCAAAGAAATAAACAACCCTAACCAACCCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGAAAATAAAATGTTGAATGAACAAGAAAAACACGCGTTTTTAAATGGCAATTACGGtatgacgcaatcgggggataagtgtaaactggtgttagtgggcgaaactcaccgagataatccgtatttggttcttatcaccagaggtgacgattggtcgttgcagtggac                                                                                     >  1:530863‑M2/1‑140 (MQ=255)                                                                                                                                          ctAGACAAATACAAGCCAAAGAAATAAACAACCCTAACCAACCCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGAAAATAAAATGTTGAATGAACAAGAAAAACACGCGTTTTTAAATGGCAATTACGGtatgacgcaatcgggggataagtgtaaactggtgttagtgggcgaaactcaccgagataatccgtatttggttcttatcaccagaggtgacgattggtcgttgcagtgga                                                                                      <  1:736135‑M2/250‑111 (MQ=255)                                                                                                                                               cAAATACAAGCCAAAGAAATAAACAACCCTAACCAACCCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGAAAATAAAATGTTGAATGAACAAGAAAAACACGCGTTTTTAAATGGCAATTACGGtatgacgcaatcgggggataagtgtaaactggtgttagtgggcgaaactcaccgagataatccgtatttggttcttatcaccagaggtgacgattggtcgttgcagtggactgac                                                                                 <  1:98372‑M2/250‑116 (MQ=255)                                                                                                                                                  aTACAAGCCAAAGAAATAAACAACCCTAACCAACCCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGAAAATAAAATGTTGAATGAACAAGAAAAACACGCGTTTTTAAATGGCAATTACGGtatgacgcaatcgggggataagtgtaaactggtgttagtgggcgaaactcaccgagataatccgtatttggttcttatcaccagaggtgacgattggtcgttgcagtggactgaccagg                                                                             <  1:784652‑M2/251‑120 (MQ=255)                                                                                                                                                    aCAAGCCAAAGAAATAAACAACCCTAACCAACCCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGAAAATAAAATGTTGAATGAACAAGAAAAACACGCGTTTTTAAATGGCAATTACGGtatgacgcaatcgggggataagtgtaaactggtgttagtgggcgaaactcaccgagataatccgtatttggttcttatcgccagaggtggcgattggtcgttgcagtggactgaccaggac                                                                           >  1:107583‑M2/1‑130 (MQ=255)                                                                                                                                                              gAAATAAACAACCCTAACCAACCCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGAAAATAAAATGTTGAATGAACAAGAAAAACACGCGTTTTTAAATGGCAATTACGGtatgacgcaatcgggggataagtgtaaactggtgttagtgggcgaaactcaccgagataatccgtatttggttcttatcaccagaggtgacgattggtcgttgcagtggactgaccaggacctatcatgtg                                                                 <  1:832155‑M2/251‑132 (MQ=255)                                                                                                                                                              gAAATAAACAACCCTAACCAACCCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGAAAATAAAATGTTGAATGAACAAGAAAAACACGCGTTTTTAAATGGCAATTACGGtatgacgcaatcgggggataagtgtaaactggtgttagtgggcgaaactcaccgagataatccgtatttggttcttatcaccagaggtgacgattggtcgttgcagtggac                                                                                     <  1:473504‑M2/231‑112 (MQ=255)                                                                                                                                                               aaaTAAACAACCCTAACCAACCCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGAAAATAAAATGTTGAATGAACAAGAAAAACACGCGTTTTTAAATGGCAATTACGGtatgacgcaatcgggggataagtgtaaactggtgttagtgggcgaaactcaccgagataatccgtatttggttcttatcaccagaggtgacgattggtcgttgcagtggactgaccaggacctatcatgtgt                                                                >  1:403018‑M2/1‑119 (MQ=255)                                                                                                                                                                  tAAACAACCCTAACCAACCCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGAAAATAAAATGTTGAATGAACAAGAAAAACACGCGTTTTTAAATGGCAATTACGGtatgacgcaatcgggggataagtgtaaactggtgttagtgggcgaaactcaccgagataatccgtatttggttcttatcaccagaggtgacgattggtcgttgcagtggactgaccaggacctatcatgtgttgt                                                             >  1:313394‑M2/1‑116 (MQ=255)                                                                                                                                                                        acCCTAACCAACCCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGAAAATAAAATGTTGAATGAACAAGAAAAACACGCGTTTTTAAATGGCAATTACGGtatgacgcaatcgggggataagtgtaaactggtgttagtgggcgaaactcaccgagataatccgtatttggttcttatcaccagaggtgacgattggtcgttgcagtggactgaccaggacctatcatgtgttgtctcg                                                         <  1:833726‑M2/249‑140 (MQ=255)                                                                                                                                                                        acCCTAACCAACCCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGAAAATAAAATGTTGAATGAACAAGAAAAACACGCGTTTTTAAATGGCAATTACGGtatgacgcaatcgggggataagtgtaaactggtgttagtgggcgaaactcaccgagataatccgtatttggttcttatcaccagaggtgacgattggtcgttgcagtggactgacc                                                                                >  1:708760‑M2/1‑110 (MQ=255)                                                                                                                                                                        acCCTAACCAACCCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGAAAATAAAATGTTGAATGAACAAGAAAAACACGCGTTTTTAAATGGCAATTACGGtatgacgcaatcgggggataagtgtaaactggtgttagtgggcgaaactcaccgagataatccgtatttggttcttatcaccagaggtgacgattggtc                                                                                                 >  1:333373‑M2/1‑110 (MQ=255)                                                                                                                                                                             aaccaaccCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGAAAATAAAATGTTGAATGAACAAGAAAAACACGCGTTTTTAAATGGCAATTACGGtatgacgcaatcgggggataagtgtaaactggtgttagtgggcgaaactcaccgagataatccgtatttggttcttatcaccagaggtgacgattggtcgttgcagtggactgaccaggac                                                                           >  1:93855‑M2/1‑105 (MQ=255)                                                                                                                                                                             aaccaaccCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGAAAATAAAATGTTGAATGAACAAGAAAAACACGCGTTTTTAAATGGCAATTACGGtatgacgcaatcgggggataagtgtaaactggtgttagtgggcgaaactcaccgagataatccgtatttggttcttatcaccagaggtgacgattggtcgttgcagtggactgaccaggac                                                                           <  1:222117‑M2/226‑122 (MQ=255)                                                                                                                                                                               ccaaccCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGAAAATAAAATGTTGAATGAACAAGAAAAACACGCGTTTTTAAATGGCAATTACGGtatgacgcaatcgggggataagtgtaaactggtgttagtgggcgaaactcaccgagataatccgtatttggttcttatcaccagaggtgacgattggtcgttgcagtggactgaccaggacctatcatgtgttgtctcgctttggcaa                                                >  1:869862‑M2/1‑103 (MQ=255)                                                                                                                                                                                  accCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGAAAATAAAATGTTGAATGAACAAGAAAAACACGCGTTTTTAAATGGCAATTACGGtatgacgcaatcgggggataagtgtaaactggtgttagtgggcgaaactcaccgagataatccgtatttggttcttatcaccagaggtgacgattggtcgttgcagtggactgaccaggacctatcatgtgttgtctcgctttggcaaga                                              <  1:782192‑M2/250‑151 (MQ=255)                                                                                                                                                                                   ccccAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGAAAATAAAATGTTGAATGAACAAGAAAAACACGCGTTTTTAAATGGCAATTACGGtatgacgcaatcgggggataagtgtaaactggtgttagtgggcgaaactcaccgagataatccgtatttggttcttatcaccagaggtgacgattggtcgttgcagtggactgaccaggacctatcatgtgttgtctcgctttggcaagaca                                            <  1:587067‑M2/251‑153 (MQ=255)                                                                                                                                                                                   ccccAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGAAAATAAAATGTTGAATGAACAAGAAAAACACGCGTTTTTAAATGGCAATTACGGtatgacgcaatcgggggataagtgtaaactggtgttagtgggcgaaactcaccgagataatccgtatttggttcttatcaccagaggtgacgattggtcgttgcagtggactgaccaggacctatcatgtgttgtctcgctttggcaagaca                                            <  1:540849‑M2/251‑153 (MQ=255)                                                                                                                                                                                          cccTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGAAAATAAAATGTTGAATGAACAAGAAAAACACGCGTTTTTAAATGGCAATTACGGtatgacgcaatcgggggataagtgtaaactggtgttagtgggcgaaactcaccgagataatccgtatttggttcttatcaccagaggtgacgattggtcgttgcagtggactgaccaggacctatcatgtgttgtctcgctttggcaagacaagcccga                                     <  1:602270‑M2/251‑160 (MQ=255)                                                                                                                                                                                            cTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGAAAATAAAATGTTGAATGAACAAGAAAAACACGCGTTTTTAAATGGCAATTACGGtatgacgcaatcgggggataagtgtaaactggtgttagtgggcgaaactcaccgagataatccgtatttggttcttatcaccagaggtgacgattggtcgttgcagtggactgaccaggacctatcatgtgttgtctcgctttggcaagacaagcccgaac                                   >  1:7423‑M2/1‑90 (MQ=255)                                                                                                                                                                                            cTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGAAAATAAAATGTTGAATGAACAAGAAAAACACGCGTTTTTAAATGGCAATTACGGtatgacgcaatcgggggataagtgtaaactggtgttagtgggcgaaactcaccgagataatccgtatttggttcttatcaccagaggtgacgattggtcgttgcagtggactgaccaggacctatcatgtgttgtctcgctttggcaagacaagcccgaac                                   >  1:115129‑M2/1‑90 (MQ=255)                                                                                                                                                                                            cTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGAAAATAAAATGTTGAATGAACAAGAAAAACACGCGTTTTTAAATGGCAATTACGGtatgacgcaatcgggggataagtgtaaactggtgttagtgggcgaaactcaccgagataatccgtatttggttcttatcaccagaggtgacgattggtcgttgcagtggactgaccaggacctatcatgtgttgtctcgctttggcaagacaagcccgaa                                    <  1:347519‑M2/250‑161 (MQ=255)                                                                                                                                                                                            cTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGAAAATAAAATGTTGAATGAACAAGAAAAACACGCGTTTTTAAATGGCAATTACGGtatgacgcaatcgggggataagtgtaaactggtgttagtgggcgaaactcaccgagataatccgtatttggttcttatcaccagaggtgacgattggtcgttgcagtggactgaccaggac                                                                           >  1:700122‑M2/1‑90 (MQ=255)                                                                                                                                                                                                        gCCTTTTTATTACTCAAAAAAGGAAAATAAAATGTTGAATGAACAAGAAAAACACGCGTTTTTAAATGGCAATTACGGtatgacgcaatcgggggataagtgtaaactggtgttagttggcgaaactcaccgagataatccgtatttggttcttatcaccagaggtgacgattggtcgt                                                                                               >  1:587807‑M2/1‑78 (MQ=255)                                                                                                                                                                                                          cTTTTTATTTCCGAAAAAAGGAAAATAAAAAGTTGAATGAAAAAGAAAAAAACCCGTTTTTCAATGGCAATTACGGtatgaggcaatccggggataagtgtaaactggtgttagtgggcgaaactccccgagataatccgtatttggttcttatcaccagaggtgacgattggtcgttgcagtggactgaccaggacctatcatgtgttgtctcgctttggcaagacaagcccgaaccgtttgatttagat                     >  1:291177‑M2/1‑76 (MQ=255)                                                                                                                                                                                                          cTTTTTATTACTCAAAAAAGGAAAATAAAATGTTGAATGAACAAGAAAAACACGCGTTTTTAAATGGCAATTACGGtatgacgcaatcgggggataagtgtaaactggtgttagtgggcgaaactcaccgagataatccgtatttggttcttatcaccagaggtgacgattggtcgttgcagtggactgaccaggacctatcatgtgttgtctcgctttggcaagacaagcccgaaccgtttgatttagat                     >  1:75066‑M2/1‑76 (MQ=255)                                                                                                                                                                                                                      cAAAAAAGGAAAATAAAATGTTGAATGAACAAGAAAAACACGCGTTTTTAAATGGCAATTACGGtatgacgcaatcgggggataagtgtaaactggtgttagtgggcgaaactcaccgagataatccgtatttggttcttatcaccagaggtgacgattggtcgttgcagtggactgaccaggacctatcatgtgttgtctcgctttggcaagacaagcccgaac                                   <  1:773311‑M2/225‑162 (MQ=255)                                                                                                                                                                                                                             ggAAAATAAAATGTTGAATGAACAAGAAAAACACGCGTTTTTAAATGGCAATTACGGtatgacgcaatcgggggataagtgtaaactggtgttagtgggcgaaactcaccgagataatccgtatttggttcttatcaccagaggtgacgattggtcgttgcagtggactgaccaggacctatcatgtgttgtctcgctttggcaagacaagcccgaaccgtttgatttagatcgggcgctgagaggcgaag  <  1:155340‑M2/251‑195 (MQ=255)                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  ATTTAAAAGATACTATTTTGTCCGTTGATACAGACAAAGCGTTTCTAATAATTTTCCCACAGTGGGAATCGCAGCTACTGGAAAGTTTGCCGCCCCGTAAAGCGGGACTACCTGCTACTCCCGCCGATGTATCTTATCTAGACAAATACAAGCCAAAGAAATAAACAACCCTAACCAACCCCAAGCCCTCCACGTGAGGGCCTTTTTATTACTCAAAAAAGGACAATAAGATGTTGAATGAACAAGAAAAACACGCGTTTTTAAATGGCAATTACGGTATTCCGTTAGTGGGACGGTATATGTCTTCTCCGGTTGCGGCAGGGCAAGTTGTTTCGGCTGTTGGGCTTCGCGGTCTAAAATATCCAGCACCCAGCGGCGGAAGTCTTTGGCTACTTTGGTGTGGCTTAACATGCCGATGAGGTGTGCGCCGCGCAAGGAGAAGATGCGCACTTTTTGGATTCCGCCATTTGT  >  1/1129447‑1129917

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑