Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,127,555	C→A	intergenic (+12065/‑11499)	unknown → / → unknown	tRNA‑Gln‑TTG/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,127,555	0	C	A	100.0%	91.6 / NA	33	intergenic (+12065/‑11499)	unknown/unknown	tRNA‑Gln‑TTG/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (23/10);  total (23/10)

GTGTTGAACGATGTTGTACATTTGATGCTAAAGTGCCCTACTTGGGTATGCTAATTACGTAAGAGCGGTAATTATATAAAACGAATTTAAATAAACAGAAAATTATATGAAATGGGGAAGTATCCCACTCGATTATTTATCGGCGATATAATGCAATGATTTATAAATGAATAAAAATGATATTGTTTATTCTCTACCGTCATTTTTACCGTCACCAGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGGCCCATAGACGCTTTAACACTTTTGGGGAATTTACCTTGTTTAATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTTGCTTTACTTAGTAGCGCTGGTTGTTGTTCCATTATTCTTCTCCATTAAAACAAAAAGCCCAT  >  1/1127362‑1127801                                                                                                                                                                                                  |                                                                                                                                                                                                                                                       gtgtTGAACGATGTTGTACATTTGATGCTAAAGTGCCCTACTTGGGTATGCTAATTACGTAAGAGCGGTAATTATATAAAACGAATTTAAATAAACAGAAAATTATATGAAATGGGGAAGTATCCCACTCGATTATTTATCGGCGACATAATGCAATGATTTATCAATGAATAAAATTGATATTGTTTATTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCa                                                                                                                                                                                               >  1:139352/1‑251 (MQ=255)                   cATTTGATGCTAAAGTGCCCTACTTGGGTATGCTAATTACGTAAGAGCGGTAATTATATAAAACGAATTTAAATAAACAGAAAATTATATGAAATGGGGAAGTATCCCACTCGATTATTTATCGGCGACATAATGCAATGATTTATCAATGAATAAAATTGATATTGTTTATTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAatat                                                                                                                                                                                    <  1:663575/244‑1 (MQ=255)                         aTGCTAAAGTGCCCTACTTGGGTATGCTAATTACGTAAGAGCGGTAATTATATAAAACGAATTTAAATAAACAGAAAATTATATGAAATGGGGAAGTATCCCACTCGATTATTTATCGGCGACATAATGCAATGATTTATCAATGAATAAAATTGATATTGTTTATTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCa                                                                                                                                                                       >  1:51365/1‑251 (MQ=255)                            cTAAAGTGCCCTACTTGGGTATGCTAATTACGTAAGAGCGGTAATTATATAAAACGAATTTAAATAAACAGAAAATTATATGAAATGGGGAAGTATCCCACTCGATTATTTATCGGCGACATAATGCAATGATTTATCAATGAATAAAATTGATATTGTTTATTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACg                                                                                                                                                                     >  1:209946/1‑250 (MQ=255)                                    cccTACTTGGGTATGCTAATTACGTAAGAGCGGTAATTATATAAAACGAATTTAAATAAACAGAAAATTATATGAAATGGGGAAGTATCCCACTCGATTATTTATCGGCGACATAATGCAATGATTTATCAATGAATAAAATTGATATTGTTTATTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACgcc                                                                                                                                                            <  1:189809/251‑3 (MQ=255)                                      cTACTTGGGTATGCTAATTACGTAAGAGCGGTAATTATATAAAACGAATTTAAATAAACAGAAAATTATATGAAATGGGGAAGTATCCCACTCGATTATTTATCGGCGACATAATGCAATGATTTATCAATGAATAAAATTGATATTGTTTATTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACgccc                                                                                                                                                           >  1:469383/1‑247 (MQ=255)                                              gTATGCTAATTACGTAAGAGCGGTAATTATATAAAACGAATTTAAATAAACAGAAAATTATATGAAATGGGGAAGCATCCCACTCGATTATTTATCGGCGACATAATGCAATGATTTATCAATGAATAAAATTGATATTGTTTATTCTATAc                                                                                                                                                                                                                                                     >  1:490196/1‑152 (MQ=255)                                                  gCTAATTACGTAAGAGCGGTAATTATATAAAACGAATTTAAATAAACAGAAAATTATATGAAATGGGGAAGTATCCCACTCGATTATTTATCGGCGACATAATGCAATGATTTATCAATGAATAAAATTGATATTGTTTATTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGCCCCATGGACGCTTTa                                                                                                                                              >  1:267202/1‑251 (MQ=255)                                                                                        taaataaaCAGAAAATTATATGAAATGGGGAAGTATCCCACTCGATTATTTATCGGCGACATAATGCAATGATTTATCAATGAATAAAATTGATATTGTTTATTCTATACCGTCATTTTTACCGTctttggaagaaaat                                                                                                                                                                                                                        >  1:521727/1‑126 (MQ=255)                                                                                                     aaTTATATGAAATGGGGAAGTATCCCACTCGATTATTTATCGGCGACATAATGCAATGATTTATCAATGAATAAAATTGATATTGTTTATTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGCCCCATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCg                                                                                           >  1:353600/1‑251 (MQ=255)                                                                                                      aTTATATGAAATGGGGAAGTATCCCACTCGATTATTTATCGGCGACATAATGCAATGATTTATCAATGAATAAAATTGATATTGTTTATTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTtctc                                                                                                                                                                         <  1:151176/172‑1 (MQ=255)                                                                                                              aaaTGGGGAAGTATCCCACTCGATTATTTATCGGCGACATAATGCAATGATTTATCAATGAATAAAATTGATATTGTTTATTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTAt                                                                                                                                                                                          >  1:94481/1‑147 (MQ=255)                                                                                                                     gAAGTATCCCACTCGATTATTTATCGGCGACATAATGCAATGATTTATCAATGAATAAAATTGATATTGTTTATTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGCCCCATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACtt                                                                           >  1:534059/1‑251 (MQ=255)                                                                                                                      aaGTATCCCACTCGATTATTTATCGGCGACATAATGCAATGATTTATCAATGAATAAAATTGATATTGTTTATTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGCCCCATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTcatcat                                                                                                              >  1:573166/1‑215 (MQ=255)                                                                                                                                  cGATTATTTATCGGCGACATAATGCAATGATTTATCAATGAATAAAATTGATATTGTTTATTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGCCCCATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTTg                                                               >  1:811968/1‑250 (MQ=255)                                                                                                                                       atttatCGGCGACATAATGCAATGATTTATCAATGAATAAAATTGATATTGTTTATTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGCCCCATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAg                                                                                     <  1:186895/223‑1 (MQ=255)                                                                                                                                       atttatCGGCGACATAATGCAATGATTTATCAATGAATAAAATTGATATTGTTTATTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGCCCCATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTTGCTTTa                                                          >  1:113098/1‑250 (MQ=255)                                                                                                                                             cGGCGACATAATGCAATGATTTATCAATGAATAAAATTGATATTGTTTATTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGCCCCATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTTGCTTTACTtagta                                                   >  1:787709/1‑251 (MQ=255)                                                                                                                                                                         gAATAAAATTGATATTGTTTATTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGCCCCATGGACGCTTTAACACTTTTGGGGAATTTACCTTGtctcatca                                                                                                               <  1:558209/163‑8 (MQ=255)                                                                                                                                                                          aaTAAAATTGATATTGTTTATTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGCCCCATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTTGCTTTACTTAGTAGCGCTGGTTGTTGTTCCATTATTCTtctc                      <  1:791741/251‑1 (MQ=255)                                                                                                                                                                               aattGATATTGTTTATTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTacac                                                                                                                                                               >  1:454635/4‑109 (MQ=255)                                                                                                                                                                                 ttGATATTGTTTATTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGCCCCATGGACGCTTTAACACTTTTGGGGAAttt                                                                                                                             >  1:787692/2‑141 (MQ=255)                                                                                                                                                                                        tGTTTATTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACGCGCCCCATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAAtt                                                                                                    <  1:844659/159‑1 (MQ=255)                                                                                                                                                                                        tGTTTATTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGCCCCATGGACGCTTTAACACTTTTGGGGAATTTAcc                                                                                                                          >  1:374983/1‑137 (MQ=255)                                                                                                                                                                                        tGTTTATTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGCCCCATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTTGCtt                                                            <  1:78960/199‑1 (MQ=255)                                                                                                                                                                                         gTTTATTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTAt                                                                                                                                                                                          >  1:772906/1‑72 (MQ=255)                                                                                                                                                                                         gTTTATTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGCCCCATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTTGCTTTACTtagtag                                                  <  1:208337/208‑1 (MQ=255)                                                                                                                                                                                         gTTTATTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGCCCCATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTTGCTTTACTTAGTAGCGCTGGTTGTTGTTCCATTATTCTTCTCCATTAAAACAAAAAg       >  1:825922/1‑251 (MQ=255)                                                                                                                                                                                             aTTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGTTTTTATCCACGCTACACGCCCCATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTTGCTTTACTTAGTAGCGCTGGTTGTTGTTCCATTATTCTTCTCCATTAAAACAAAAAGCCCa   >  1:412077/1‑251 (MQ=255)                                                                                                                                                                                             aTTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGCCCCATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTTGCtt                                                            <  1:730566/194‑1 (MQ=255)                                                                                                                                                                                             aTTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGCCCCATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTTGCTTTACTTAGTAGCGCTGGTTGTTGTTCCATTATTCTTCTCCATTAAAACAAAAAGCCCa   >  1:340052/1‑251 (MQ=255)                                                                                                                                                                                             aTTCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGCCCAATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCt                                                                                   >  1:361858/1‑171 (MQ=255)                                                                                                                                                                                              ttCTATACCGTCATTTTTACCGTCTTTGGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGCCCCATGGACGCTTTAACACTTTTGGGGAATTTACCTTGTCTCATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTTGCTTTACTTAGTAGCGCTGGTTGTTGTTCCATTATTCTTCTCCATTAAAACAAAAAGCCCAt  >  1:488936/1‑251 (MQ=255)                                                                                                                                                                                                  |                                                                                                                                                                                                                                                       GTGTTGAACGATGTTGTACATTTGATGCTAAAGTGCCCTACTTGGGTATGCTAATTACGTAAGAGCGGTAATTATATAAAACGAATTTAAATAAACAGAAAATTATATGAAATGGGGAAGTATCCCACTCGATTATTTATCGGCGATATAATGCAATGATTTATAAATGAATAAAAATGATATTGTTTATTCTCTACCGTCATTTTTACCGTCACCAGAAGAAAATGCCATCAAGAACGTTCAGCAAGCCAAGTATCAATATCAGATTTTCTCCACGCTACACGGCCCATAGACGCTTTAACACTTTTGGGGAATTTACCTTGTTTAATCATACGCCAAATTGTTGTGTCGCTCAAGCTGGTGACTTCAATCACCTTTGCTTTACTTAGTAGCGCTGGTTGTTGTTCCATTATTCTTCTCCATTAAAACAAAAAGCCCAT  >  1/1127362‑1127801

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑