Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,121,433	A→G	intergenic (+5943/‑17621)	unknown → / → unknown	tRNA‑Gln‑TTG/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,121,433	0	A	G	100.0%	172.0 / NA	60	intergenic (+5943/‑17621)	unknown/unknown	tRNA‑Gln‑TTG/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/27);  total (33/27)

GAATCCGTTGCCGCTGCCAAGGTGGCACGTTCAAACCTTGCCATACTTTTTTTATGTCCTGATTCACGCCTTGTTGTGTTAATTTAACTTTGCCCGAATAACCGAAACGGATCCAAACTTTCGTTCCCGCTAACGGTAAACCGAGGGTTTCTTTATATTCCTGCCCATTTTCATCGCGCCAAAGTGCGGTCATTTTTTCCAGTGTTTTTTTCAGGCTTAACGTGCCCAAGTTGTCCAGCAAATTCATGGGATCGTCAAATTGGGCTTCCAAACAGACTGGCGAAATATCCGTAAATACCGGCGTGAGAAACAATCGGTTTTGATAACGGCGAAGGATCTTATCCTCCAAACGAAACTGCGGCTGCGCATCGCTTTTGGCGAAAATGACATCGGAAATGAGCTGCTCCAGTTGTGCCAATGAGGGCATCATCACACCCTGTTCCTGCAACCATAAACGCAGCAA  >  1/1121197‑1121659                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                   gAATCCGTTGCCGCTGCCAAGGTGGCACGTTCAAACCTTGCCATACTTTTTTTATGTCCTGATTCACGCCTTGTTGTGTTAATTTAACTTTGCCCGAATAACCGAAACGGATCCAAACTTTCGTTCCCGCTAACGGTAAACCGAGGGTTTCTTTATATTCCTGCCCATTTTCATCGCGCCAAAGTGCGGTCATTTTTTCCAGTGTTTTTTTCAGGCTTAACGTGCCCAAGTTGTCCGGCAAATTCATggg                                                                                                                                                                                                                       <  1:371932/250‑1 (MQ=255)            cGCTGCCAAGGTGGCACGTTCAAACCTTGCCATACTTTTTTTATGTCCTGATTCACGCCTTGTTGTGTTAATTTAACTTTGTCCGAATAACCGAAACGGATCCAAACTTTCGTTCCCGCTAACGGTAAACCGAGGGTTTCTTTATATTCCTGCCCATTTTCATCGCGCCAAAGTGCGGTCATTTTTTCCAGTGTTTTTTTCAGGCTTAACGTGCCCAAGTTGTCCGGCAAATTCATGGGATCGTCAAATTg                                                                                                                                                                                                           <  1:440594/251‑1 (MQ=255)              cTGCCAAGGTGGCACGTTCAAACCTTGCCATACTTTTTTTATGTCCTGATTCACGCCTTGTTGTGTTAATTTAACTTTGCCCGAATAACCGAAACGGATCCAAACTTTCGTTCCCGCTAACGGTAAACCGAGGGATTCTTTTTATTCCTGCCCATTTTCATCGCGCCAAAGCGCGGTCCTTTTTTCCAGTGTTTTTTTCCGGCTTAACGTGCCCAAGTTGTCCGGCAAATTCATGGGATCGTCAAATTggg                                                                                                                                                                                                         >  1:668675/1‑251 (MQ=255)                            cGTTCAAACCTTGCCATACTTTTTTTATGTCCTGATTCACGCCTTGTTGTGTTAATTTAACTTTGCCCGAATAACCGAAACGGATCCAAACTTTCGTTCCCGCTAACGGTAAACCGAGGGTTTCTTTATATTCCTGCCCATTTTCATCGCGCCAAAGTGCGGTCATTTTTTCCAGTGTTTTTTTCAGGCTTAACGTGCCCAAGTTGTCCGGCAAATTCATGGGATCGTCAAATTGGGCTTCCAAACAGa                                                                                                                                                                                             >  1:888173/1‑249 (MQ=255)                                 aaaCCTTGCCATACTTTTTTTATGTCCTGATTCACGCCTTGTTGTGTTAATTTAACTTTGCCCGAATAACCGAAACGGATCCAAACTTTCGTTCCCGCTAACGGTAAACCGAGGGTTTCTTTATATTCCTGCCCATTTTCATCGCGCCAAAGTGCGGTCATTTTTTCCAGTGTTTTTTTCAGGCTTAACGTGCCCAAGTTGTCCGGCAAATTCATGGGATCGTCAAATTGGGCTTCCAAACAGACTGGCGa                                                                                                                                                                                      <  1:49485/251‑1 (MQ=255)                                     cTTGACATACTTTGTTTATGTCCTGATTCACGACTTGTTGTTTTAATTTAACTTTGCCCGAATAACCGAAACGGATCCAACCTTTCGTTCCCGCTAACGGTAAACCGAGGGTTTCTTTATATTCCTGCCCATTTTCATCGCGCCAAAGTGCGGTCATTTTTTCCAGTGTTGTTTTCAGGCTTAACGTGCCCAAGTTGTCCGGCAAATTCATGGGATCGTCAAATTGGGCTTCCAAACAGACTGGCGAAata                                                                                                                                                                                  <  1:70335/251‑1 (MQ=255)                                            tACTTTTTTTATGTCCTGATTCACGCCTTGTTGTGTTAATTTAACTTTGCCCGAATAACCGAAACGGATCCAAACTTTCGTTCCCGCTAACGGTAAACCGAGGGTTTCTTTATATTCCTGCCCATTTTCATCGCGCCAAAGTGCGGTCATTTTTTCCAGTGTTTTTTTCAGGCTTAACGTGCCCAAGTTGTCCGGCAAATTCATGGGATCGTCAAATTGGGCTTCCAAACAGACTGGCGAAATATCCGTaa                                                                                                                                                                           <  1:82137/251‑1 (MQ=255)                                             aCTTTTTTTATGTCCTGATTCACGCCTTGTTGTGTTAATTTAACTTTGCCCGAATAACCGAAACGGATCCAAACTTTCGTTCCCGCTAACGGTAAACCGAGGGTTTCTTTATATTCCTGCCCATTTTCATCGCGCCAAAGTGCGGTCATTTTTTCCAGTGTTTTTTTCAGGCTTAACGTGCCCAAGTTGTCCGGCAAATTCATGGGATCGTCAAATTGGGCTTCCAAACAGACTGGCGAAATATCCGTaaa                                                                                                                                                                          <  1:458634/251‑1 (MQ=255)                                              cTTTTTTTATGTCCTGATTCACGCCTTGTTGTGTTAATTTAACTTTGCCCGAATAATCGAAACGGATCCAAACTTTCGTTCCCGCTAACGGTAAACCGAGGGTTTCTTTATATTCCTGCCCATTTTCATCGCGCCAAAGTGCGGTCATTTTTTCCAGTGTTTTTTTCAGGCTTAACGTGCCCAAGTTGTCCGGCAAATTCATGGGATCGTCAAATTGGGCTTCCaa                                                                                                                                                                                                  <  1:442768/226‑1 (MQ=255)                                                ttttttATGTCCTGATTCACGCCTTGTTGTGTTAATTTAACTTTGCCCGAATAACCGAAACGGATCCAAACTTTCGTTCCCGCTAACGGTAAACCGAGGGTTTCTTTATATTCCTGCCCATTTTCATCGCGCCAAAGTGCGGTCATTTTTTCCAGTGTTTTTTTCAGGCTTAACGTGCCCAAGTTGTCCGGCAAATTCATggg                                                                                                                                                                                                                       <  1:585370/203‑1 (MQ=255)                                                ttttttATGTCCTGATTCACGCCTTGTTGTGTTAATTTAACTTTGCCCGAATAACCGAAACGGATCCAAACTTTCGTTCCCGCTAACGGTAAACCGAGGGTTTCTTTATATTCCTGCCCATTTTCATCGCGCCAAAGTGCGGTCATTTTTTCCAGTGTTTTTTTCAGGCTTAACGTGCCCAAGTTGTCCGGCAAATTCATGGGATCGTCAAATTGGGCTTCCAAACAGACTGGCGAAATATCCGTAAATa                                                                                                                                                                        >  1:859462/1‑250 (MQ=255)                                                 tttttATGTCCTGATTCACGCCTTGTTGTGTTAATTTAACTTTGCCCGAATAACCGAAACGGATCCAAACTTTCGTTCCCGCTAACGGTAAACCGAGGGTTTCTTTATATTCCTGCCCATTTTCATCGCGCCAAAGTGCGGTCATTTTTTCCAGTGTTTTTTTCAGGCTTAACGTGCCCAAGTTGTCCGGCAAATTCATGGGATCGTCAAATTGGGCTTCCAAACAGACTGGCGAAATATCCGTAAATAcc                                                                                                                                                                      <  1:485763/251‑1 (MQ=255)                                                   tttATGTCCTGATTCACGCCTTGTTGTGTTAATTTAACTTTGCCCGAATAACCGAAACGGATCCAAACTTTCGTTCCCGCTAACGGTAAACCGAGGGTTTCTTTATATTCCTGCCCATTTTCATCGCGCCAAAGTGCGGTCATTTTTTCCAGTGTTTTTTTCAGGCTTAACGTGCCCAAGTTGTCCGGCAAATTCATGGGATCGTCAAATTGGGCTTCCAAACAGACTGGCGaaa                                                                                                                                                                                    <  1:882245/235‑1 (MQ=255)                                                   tttATGTCCTGATTCACGCCTTGTTGTGTTAATTTAACTTTGCCCGAATAACCGAAACGGATCCAAACTTTCGTTCCCGCTAACGGTAAACCGAGGGTTTCTTTATATTCCTGCCCATTTTCATCGCGCCAAAGTGCGGTCATTTTTTCCAGTGTTTTTTTCAGGCTTAACGTGCCCAAGTTGTCCGGCAAATTCATGGGATCGTCAAATTGGGCTTCCAAACAGACTGGCGAAATATCCGTACATACCgg                                                                                                                                                                    >  1:829004/1‑251 (MQ=255)                                                   tttATGTCCTGATTCACGCCTTGTTGTGTTAATTTAACTTTGCCCGAATAACCGAAACGGATCCAAACTTTCGTTCCCGCTAACGGTAAACCGAGGGTTTCTTTATATTCCTGCCCATTTTCATCGCGCCAAAGTGCGGTCATTTTTTCCAGTGTTTTTTTCAGGCTTAACGTGCCCAAGTTGTCCGGCAAATTCATGGGATCGTCAAATTGGGCTTCCAAACAGACTGGCGAAATATCCGTAAATACCgg                                                                                                                                                                    >  1:172581/1‑251 (MQ=255)                                                           cTGATTCACGCCTTGTTGTGTTAATTTAACTTTGCCCGAATAACCGAAACGGATCCAAACTTTCGTTCCCGCTAACGGTAAACCGAGGGTTTCTTTATATTCCTGCCCATTTTCATCGCGCCAAAGTGCGGTCATTTTTTCCAGTGTTTTTTTCAGGCTTAACGTGCCCAAGTTGTCCGGCAAATTCATGGGATCGTCAAATTGGGCTTCCAAACAGACTGGCGAAATATCCGTAAATACCGGCGTGAGaa                                                                                                                                                            <  1:28373/251‑1 (MQ=255)                                                             gATTCACGCCTTGTTGTGTTAATTTAACTTTGCCCGAATAACCGAAACGGATCCAAACTTTCGTTCCCGCTAACGGTAAACCGAGGGTTTCTTTATATTCCTGCCCATTTTCATCGCGCCAAAGTGCGGTCATTTTTTCCAGTGTTTTTTTCAGGCTTAACGTGCCCAAGTTGTCCGGCAAATTCATGGGATCGTCAAATTGGGCTTCCAAACAGACTGGCGAAATATCCGTAAATACCGGCGTGAGaaa                                                                                                                                                           >  1:897768/1‑250 (MQ=255)                                                               ttCACGCCTTGTTGTGTTAATTTAACTTTGCCCGAATAACCGAAACGGATCCAAACTTTCGTTCCCGCTAACGGTAAACCGAGGGTTTCTTTATATTCCTGCCCATTTTCATCGCGCCAAAGTGCGGTCATTTTTTCCAGTGTTTTTTTCAGGCTTAACGTGCCCAAGTTGTCCGGCAAATTCATGGGATCGTCAAATTGGGCTTCCAAACAGACTGGCGAAATATCCGTAAATACCGGCGTGAGAAACaa                                                                                                                                                        >  1:381108/1‑251 (MQ=255)                                                                   cGCCTTGTTGTGTTAATTTAACTTTGCCCGAATAACCGAAACGGATCCAAACTTTCGTTCCCGCTAACGGTAAACCGAGGGTTTCTTTATATTCCTGCCCATTTTCATCGCGCCAAAGTGCGGTCATTTTTTCCAGTGTTTTTTTCAGGCTTAACGTGCCCAAGTTGTCCGGCAAATTCATGGGATCGTCAAATTGGGCTTCCAAACAGACTGGCGAAATATCCGTAAATACCGGCGTGAGAAACAATCgg                                                                                                                                                    >  1:780594/1‑251 (MQ=255)                                                                   cGCCTTGTTGTGTTAATTTAACTTTGCCCGAATAACCGAAACGGATCCAAACTTTCGTTCCCGCTAACGGTAAACCGAGGGTTTCTTTATATTCCTGCCCATTTTCATCGCGCCAAAGTGCGGTCATTTTTTCCAGTGTTTTTTTCAGGCTTAACGTGCCCAAGTTGTCCGGCAAATTCATGGGATCGTCAAATTGGGCTTCCAAACAGACTGGCGAAATATCCGTAAATACCGGCGTGAGAAACAATCgg                                                                                                                                                    >  1:605401/1‑251 (MQ=255)                                                                                  aTTTAACTTTGCCCGAATAACCGAAACGGATCCAAACTTTCGTTCCCGCTAACGGTAAACCGAGGGTTTCTTTATATTCCTGCCCATTTTCATCGCGCCAAAGTGCGGTCATTTTTTCCAGTGTTTTTTTCAGGCTTAACGTGCCCAAGTTGTCCGGCAAATTCATGGGATCGTCAAATTGGGCTTCCAAACAg                                                                                                                                                                                              <  1:597877/194‑1 (MQ=255)                                                                                      aaCTTTGCCCGAATAACCGAAACGGATCCAAACTTTCGTTCCCGCTAACGGTAAACCGAGGGTTTCTTTATATTCCTGCCCATTTTCATCGCGCCAAAGTGCGGTCATTTTTTCCAGTGTTTTTTTCAGGCTTAACGTGCCCAAGTTGTCCGGCAAATTCATGGGATCGTCAAATTGGGCTTCCAAACAGACTGGCGAAATATCCGTAAATACCGGCGTGAGAAACAATCGGTTTTGATa                                                                                                                                            >  1:124631/1‑240 (MQ=255)                                                                                             cccGAATAACCGAAACGGATCCAAACTTTCGTTCCCGCTAACGGTAAACCGAGGGTTTCTTTATATTCCTGCCCATTTTCATCGCGCCAAAGTGCGGTCATTTTTTCCAGTGTTTTTTTCAGGCTTAACGTGCCCAAGTTGTCCGGCAAATTCATGGGATCGTCAAATTGGGCTTCCAAACAGACTGGCGAAATATCCGTAAATACCGGCGTGAGAAACAATCGGTTTTGATAACGGCGAAGGATCTTAt                                                                                                                           <  1:681074/250‑1 (MQ=255)                                                                                             cccGAATAACCGAAACGGATCCAAACTTTCGTTCCCGCTAACGGTAAACCGAGGGTTTCTTTATATTCCTGCCCATTTTCATCGCGCCAAAGTGCGGTCATTTTTTCCAGTGTTTTTTTCAGGCTTAACGTGCCCAAGTTGTCCGGCAAATTCATGGGATCGTCAAATTGGGCTTCCAAACAGACTGGCGAAATATCCGTAAATACCGGCGTGAGAAACAATCGGTTTTGATAACGGCGAAGGATCTTAt                                                                                                                           <  1:623798/250‑1 (MQ=255)                                                                                              ccGAATAACCGAAACGGATCCAAACTTTCGTTCCCGCTAACGGTAAACCGAGGGTTTCTTTATATTCCTGCCCATTTTCATCGCGCCAAAGTGCGGTCATTTTTTCCAGTGTTTTTTTCAGGCTTAACGTGCCCAAGTTGTCCGGCAAATTCATGGGATCGTCAAATTGGGCTTCCAAACAGACTGGCGAAATATCCGTAAATACCGGCGTGAGAAACAATCGGTTTTGATAACGGCGAAGGATCTTAtcc                                                                                                                         <  1:552571/251‑1 (MQ=255)                                                                                                 aaTAACCGAAACGGATCCAAACTTTCGTTCCCGCTAACGGTAAACCGAGGGTTTCTTTATATTCCTGCCCATTTTCATCGCGCCAAAGTGCGGTCATTTTTTCCAGTGTTTTTTTCAGGCTTAACGTGCCCAAGTTGTCCGGCAAATTCATGGGATCGTCAAATTGGGCTTCCAAACAGACTGGCGAAATATCCGTAAATACCGGCGTGAGAAACAATCGGTTTTGATAACGGCGAAGGATCTTAtcctcc                                                                                                                      <  1:788331/251‑1 (MQ=255)                                                                                                    aaCCGAAACGGATCCAAACTTTCGTTCCCGCTAACGGTAAACCGAGGGTTTCTTTATATTCCTGCCCATTTTCATCGCGCCAAAGTGCGGTCATTTTTTCCAGTGTTTTTTTCAGGCTTAACGTGCCCAAGTTGTCCGGCAAATTCATGGGATCGTCAAATTGGGCTTCCAAACAGACTGGCGAAATATCCGTAAATACCGGCGTGAGAAACAATCGGTTTTGATAACGGCGAAGGATCTTATCCTCCaaa                                                                                                                   >  1:753705/1‑251 (MQ=255)                                                                                                     aCCGAAACGGATCCAAACTTTCGTTCCCGCTAACGGTAAACCGAGGGTTTCTTTATATTCCTGCCCATTTTCATCGCGCCAAAGTGCGGTCATTTTTTCCAGTGTTTTTTTCAGGCTTAACGTGCCCAAGTTGTCCGGCAAATTc                                                                                                                                                                                                                            >  1:432621/1‑145 (MQ=255)                                                                                                         aaaCGGATCCAAACTTTCGTTCCCGCTAACGGTAAACCGAGGGTTTCTTTATATTCCTGCCCATTTTCATCGCGCCAAAGTGCGGTCATTTTTTCCAGTGTTTTTTTCAGGCTTAACGTGCCCAAGTTGTCCGGCAAATTCATGGGATCGTCAAATTGGGCTTCCAAACAGACTGGCGAAATATCCGTAAATACCGGCGTGAGAAACAATCGGTTTTGATAACGGCGAAGGa                                                                                                                                 <  1:859251/232‑1 (MQ=255)                                                                                                                tCCAAACTTTCGTTCCCGCTAACGGTAAACCGAGGGTTTCTTTATATTCCTGCCCATTTTCATCGCGCCAAAGTGCGGTCATTTTTTCCAGTGTTTTTTTCAGGCTTAACGTGCCCAAGTTGTCCGGCAAATTCATGGGATCGTCAAATTGGGCTTCCAAACAGACTGGCGAAATATCCGTAAATACCGGCGTGAGAAACAATCGGTTTTGATAACGGCGAAGGATCTTATCCTCCAAACGAAACTGCGGc                                                                                                       <  1:778629/251‑1 (MQ=255)                                                                                                                          cGTTCCCGCTAACGGTAAACCGAGGGTTTCTTTATATTCCTGCCCATTTTCATCGCGCCAAAGTGCGGTCATTTTTTCCAGTGTTTTTTTCAGGCTTAACGTGCCCAAGTTGTCCGGCAAATTCATGGGATCGTCAAATTGGGCTTCCAAACAGACTGGCGAAATATCCGTAAATACCGGCGTGAGAAACAATCGGTTTTGATAACGGCGAAGGATCTTATCCTCCAAACGAAACTGCGGCTGCGCATCGc                                                                                             <  1:266640/251‑1 (MQ=255)                                                                                                                                cGCTAACGGTAAACCGAGGGTTTCTTTATATTCCTGCCCATTTTCATCGCGCCAAAGTGCGGTCATTTTTTCCAGTGTTTTTTTCAGGCTTAACGTGCCCAAGTTGTCCGGCAAATTCATGGGATCGTCAAATTGGGCTTCCAAACAGACTGGCGAAATATCCGTAAATACCGGCGTGAGAAACAATCGGTTTTGATAACGGCGAAGGATCTTAtcc                                                                                                                         <  1:835522/217‑1 (MQ=255)                                                                                                                                  cTAACGGTAAACCGAGGGTTTCTTTATATTCCTGCCCATTTTCATCGCGCCAAAGTGCGGTCATTTTTTCCAGTGTTTTTTTCAGGCTTAACGTGCCCAAGTTGTCCGGCAAATTCATGGGATCGTCAAATTGGGCTTCCAAACAGACTGGCGAAATATCCGTAAATACCGGCGTGAGAAACAATCGGTTTTGATAACGGCGAAGGATCTTATCCTCCAAACGAAACTGCGGCTGCGCATCGCTTTTGGCg                                                                                     <  1:466763/251‑1 (MQ=255)                                                                                                                                    aaCGGTAAACCGAGGGTTTCTTTATATTCCTGCCCATTTTCATCGCGCCAAAGTGCGGTCATTTTTTCCAGTGTTTTTTTCAGGCTTAACGTGCCCAAGTTGTCCGGCAAATTCATGGGATCGTCAAATTGGGCTTCCAAACAGACTGGCGAAATATCCGTAAATACCGGCGTGAGAAACAATCGGTTTTGATAACGGCGAAGGATCTTATCCTCCAAACGAAACTGCGGCTGCGCATCGCTTTTGGCGaa                                                                                   >  1:146822/1‑251 (MQ=255)                                                                                                                                    aaCGGTAAACCGAGGGTTTCTTTATATTCCTGCCCATTTTCATCGCGCCAAAGTGCGGTCATTTTTTCCAGTGTTTTTTTCAGGCTTAACGTGCCCAAGTTGTCCGGCAAATTCATGGGATCGTCAAATTGGGCTTCCAAACAGACTGGCGAAATATCCGTAAATACCGGCGTGAGAAACAATCGGTTTTGATAACGGCGAAGGATCTTATCCTCCAAACGAAACTGCGGCTGCGCATCGCTTTTGGCGaa                                                                                   >  1:746389/1‑251 (MQ=255)                                                                                                                                      cGGTAAACCGAGGGTTTCTTTATATTCCTGCCCATTTTCATCGCGCCAAAGTGCGGTCATTTTTTCCAGTGTTTTTTTCAGGCTTAACGTGCCCAAGTTGTCCGGCAAATTCATGGGATCGTCAAATTGGGCTTCCAAAc                                                                                                                                                                                                >  1:30056/1‑140 (MQ=255)                                                                                                                                           aaCCGAGGGTTTCTTTATATTCCTGCCCATTTTCATCGCGCCAAAGTGCGGTCATTTTTTCCAGTGTTTTTTTCAGGCTTAACGTGCCCAAGTTGTCCGGCAAATTCATGGGATCGTCAAATTGGGCTTCCAAACAGACTGGCGAAATATCCGTAAATACCGGCGTGAGAAACAATCGGTTTTGATAACGGCGAAGGATCTTATCCTCCAAACGAAACTGCGGCTGCGCATCGCTTTTGGCGAAAATGACa                                                                            >  1:666408/1‑251 (MQ=255)                                                                                                                                                    tttCTTTATATTCCTGCCCATTTTCATCGCGCCAAAGTGCGGTCATTTTTTCCAGTGTTTTTTTCAGGCTTAACGTGCCCAAGTTGTCCGGCAAATTCATGGGATCGTCAAATTGGGCTTCCAAACAGACTGGCGAAATATCCGTAAATACCGGCGTGAGAAACAATCGGTTTTGATAACGGCGAAGGATCTTATCCTCCAAACGAAACTGCGGCTGCGCATCGCTTTTGGCGAAAATGACATCGGAAATg                                                                   >  1:649958/1‑251 (MQ=255)                                                                                                                                                      tCTTTATATTCCTGCCCATTTTCATCGCGCCAAAGTGCGGTCATTTTTTCCAGTGTTTTTTTCAGGCTTAACGTGCCCAAGTTGTCCGGCAAATTCATGGGATCGTCAAATTGGGCTTCCAAACAGACTGGCGAAATATCCGTAAATACCGGCGTGAGAAACAATCGGTTTTGATAACGGCGAAGGATCTTATCCTCCAAACGAAACTGCGGCTGCGCATCGCTTTTGGCGAAAATGACATCGGAAATGAg                                                                 >  1:893137/1‑251 (MQ=255)                                                                                                                                                       cTTTATATTCCTGCCCATTTTCATCGCGCCAAAGTGCGGTCATTTTTTCCAGTGTTTTTTTCAGGCTTAACGTGCCCAAGTTGTCCGGCAAATTCATGGGATCGTCAAATTGGGCTTCCAAACAGACTGGCGAAATATCCGTAAATACCGGCGTGAGAAACAATCGGTTTTGATAACGGCGAAGGATCTTATCCTCCAAACGAAACTGCGGCTGCGCATCGCTTTTGGCGAAAATGACATCGGAAATGAg                                                                 >  1:507607/1‑250 (MQ=255)                                                                                                                                                         ttATATTCCTGCCCATTTTCATCGCGCCAAAGTGCGGTCATTTTTTCCAGTGTTTTTTTCAGGCTTAACGTGCCCAAGTTGTCCGGCAAATTCATGGGATCGTCAAATTGGGCTTCCAAACAGACTGGCGAAATATCCGTAAATACCGGCGTGAGAAACAATCGGTTTTGATAACGGCGAAGGATCTTAtcc                                                                                                                         <  1:302324/192‑1 (MQ=255)                                                                                                                                                         ttATATTCCTGCCCATTTTCATCGCGCCAAAGTGCGGTCATTTTTTCCAGTGTTTTTTTCAGGCTTAACGTGCCCAAGTTGTCCGGCAAATTCATGGGATCGTCAAATTGGGCTTCCAAACAGACTGGCGAAATATCCGTAAATACCGGCGTGAGAAACAATCGGTTTTGATAACGGCGAAGGATCTTATCCTCCAAACGAAACTGCGGCTGCGCATCGCTTTTGGCGAAAATGACATCGGAAATGAg                                                                 >  1:595357/1‑248 (MQ=255)                                                                                                                                                         ttATATTCCTGCCCATTTTCATCGCGCCAAAGTGCGGTCATTTTTTCCAGTGTTTTTTTCAGGCTTAACGTGCCCAAGTTGTCCGGCAAATTCATGGGATCGTCAAATTGGGCTTCCAAACAGACTGGCGAAATATCCGTAAATACCGGCGTGAGAAACAATCGGTTTTGATAACGGCGAAGGATCTTATCCTCCAAACGAAACTGCGGCTGCGCATCGCTTTTGGCGAAAATGACATCGGAAATGAg                                                                 >  1:359312/1‑248 (MQ=255)                                                                                                                                                            tatTCCTGCCCATTTTCATCGCGCCAAAGTGCGGTCATTTTTTCCAGTGTTTTTTTCAGGCTTAACGTGCCCAAGTTGTCCGGCAAATTCATGGGATCGTCAAATTGGGCTTCCAAACAGACTGGCGAAATATCCGTAAATACCGGCGTGAGAAACAATCGGTTTTGATAACGGCGAAGGATCTTATCCTCCAAACGAAACTGCGGCTGCGCATCGCTTTTGGCGAAAATGACATCGGAAATGAGCTGCTc                                                           <  1:165782/251‑1 (MQ=255)                                                                                                                                                             atTCCTGCCCATTTTCATCGCGCCAAAGTGCGGTCATTTTTTCCAGTGTTTTTTTCAGGCTTAACGTGCCCAAGTTGTCCGGCAAATTCATGGGATCGTCAAATTGGGCTTCCAAACAGACTGGCGAAATATCCGTAAATACCGGCGTGAGAAACAATCGGTTTTGATAACGGCGAAGGATCTTATCCTCCAAACGAAACTGCGGCTGCGCATCGCTTTTGGCGAAAATGACAt                                                                           >  1:358292/1‑234 (MQ=255)                                                                                                                                                              ttCCTGCCCATTTTCATCGCGCCAAAGTGCGGTCATTTTTTCCAGTGTTTTTTTCAGGCTTAACGTGCCCAAGTTGTCCGGCAAATTCATGGGATCGTCAAATTGGGCTTCCAAACAGACTGGCGAAATATCCGTAAATACCGGCGTGAGAAACAATCGGTTTTGATAACGGCGAAGGATCTTATCCTCCAAACGAAACTGCGGCTGCGCATCGCTTTTGGCGAAAATGACATCGGAAATGAGCTGCTCCa                                                         >  1:550520/1‑251 (MQ=255)                                                                                                                                                                  tGCCCATTTTCATCGCGCCAAAGTGCGGTCATTTTTTCCAGTGTTTTTTTCAGGCTTAACGTGCCCAAGTTGTCCGGCAAATTCATGGGATCGTCAAATTGGGCTTCCAAACAGACTGGCGAAATATCCGTAAATACCGGCGTGAGAAACAATCGGTTTTGATAACGGCGAAGGATCTTATCCTCCAAACGAAACTGCGGCTGCGCATCGCTTTTGGCGAAAATGACATCGGAAATGAGCTGCTCCAGTtg                                                     <  1:402383/251‑1 (MQ=255)                                                                                                                                                                   gCCCATTTTCATCGCGCCAAAGTGCGGTCATTTTTTCCAGTGTTTTTTTCAGGCTTAACGTGCCCAAGTTGTCCGGCAAATTCATGGGATCGTCAAATTGGGCTTCCAAACAGACTGGCGAAATATCCGTAAATACCGGCGTGAGAAACAATCGGTTTTGATAACGGCGAAGGATCTTATCCTCCAAACGAAACTGCGGCTGCGCATCGCTTTTGGCGAAAATGACATCGGAAATGAgc                                                                >  1:790725/1‑239 (MQ=255)                                                                                                                                                                      cATTTTCATCGCGCCAAAGTGCGGTCATTTTTTCCAGTGTTTTTTTCAGGCTTAACGTGCCCAAGTTGTCCGGCAAATTCATGGGATCGTCAAATTGGGCTTCCAAACAGACTGGCGAAATATCCGTAAAt                                                                                                                                                                         >  1:877081/1‑131 (MQ=255)                                                                                                                                                                      cATTTTCATCGCGCCAAAGTGCGGTCATTTTTTCCAGTGTTTTTTTCAGGCTTAACGTGCCCAAGTTGTCCGGCAAATTCATGGGATCGTCAAATTGGGCTTCCAAACAGACTGGCGAAATATCCGTAAATACCGGCGTGAGAAACAATCGGTTTTGATAACGGCGAAGGATCTTATCCTCCAAACGAAACTGCGGCTGCGCATCGCTTTTGGCg                                                                                     <  1:114906/215‑1 (MQ=255)                                                                                                                                                                      cATTTTCATCGCGCCAAAGTGCGGTCATTTTTTCCAGTGTTTTTTTCAGGCTTAACGTGCCCAAGTTGTCCGGCAAATTCATGGGATCGTCAAATTGGGCTTCCAAACAGACTGGCGAAATATCCGTAAATACCGGCGTGAGAAACAATCGGTTTTGATAACGGCGAAGGATCTTATCCTCCAAACGAAACTGCGGCTGCGCATCGCTTTTGGCGAAAATGACATCGGAAATGAGCTGCTCCAGTTGTGc                                                  >  1:165716/1‑250 (MQ=255)                                                                                                                                                                      cATTTTCATCGCGCCAAAGTGCGGTCATTTTTTCCAGTGTTTTTTTCAGGCTTAACGTGCCCAAGTTGTCCGGCAAATTCATGGGATCGTCAAATTGGGCTTCCAAACAGACTGGCGAAATATCCGTAAATACCGGCGTGAGAAACAATCGGTTTTGATAACGGCGAAGGATCTTATCCTCCAAACGAAACTGCGGCTGCGCATCGCTTTTGGCGAAAATGACATCGGAAATGAGCTGCTCCAGTTGTGc                                                  >  1:319579/1‑250 (MQ=255)                                                                                                                                                                                     aaaGTGCGGTCATTTTTTCCAGTGTTTTTTTCAGGCTTAACGTGCCCAAGTTGTCCGGCAAATTCATGGGATCGTCAAATTGGGCTTCCAAACAGACTGGCGAAATATCCGTAAATACCGGCGTGAGAAACAATCGGTTTTGATAACGGCGAAGGATCTTAtcc                                                                                                                         >  1:794435/1‑164 (MQ=255)                                                                                                                                                                                              ggtgggttttttccgcgtttttCAGGCTTAACGTGCCCAAGTTGTCCGGCAAATTCATGGGATCGTCAAATTGGGCTTCCAAACAGACTGGCGAAATATCCGTAAATACCGGCGTGAGAAACAATCGGTTTTGATAACGGCGAAGGATCTTATCCTCCAAACGAAACTGCGGCTGCGCATCGCTTTTGGCGAAAATGACATCGGAAATGAGCTGCTCCAGTTGTGCCAATGAGGGCATCATCACAcc                             >  1:561278/18‑247 (MQ=255)                                                                                                                                                                                                   ttttCCAGTGTTTTTTTCAGGCTTAACGTGCCCAAGTTGTCCGGCAAATTCATGGGATCGTCAAATTGGGCTTCCAAACAGACTGGCGAAATATCCGTAAATACCGGCGTGAGAAACAATCGGTTTTGATAACGGCGAAGGATCTTATCCTCCAAACGAAACTGCGGCTGCGCATCGCTTTTGGCGAAAATGACATCGGAAATGAGCTGCTCCAGTTGTGCCAATGAGGGCATCATCACACCCTGTTCCTg                    <  1:894524/251‑1 (MQ=255)                                                                                                                                                                                                            gtTTTTTTCAGGCTTAACGTGCCCAAGTTGTCCGGCAAATTCATGGGATCGTCAAATTGGGCTTCCAAACAGACTGGCGAAATATCCGTAAATACCGGCGTGAGAAACAATCGGTTTTGATAACGGCGAAGGATCTTATCCTCCAAACGAAACTGCGGCTGCGCATCGCTTTTGGCGAAAATGACATCGGAAATGAGCTGCTCCAGTTGTGCCAATGAGGGCATCATCACACCCTGTTcc                      <  1:80252/240‑1 (MQ=255)                                                                                                                                                                                                                      ggCTTAACGTGCCCAAGTTGTCCGGCAAATTCATGGGATCGTCAAATTGGGCTTCCAAACAGACTGGCGAAATATCCGTAAAt                                                                                                                                                                         >  1:461915/1‑83 (MQ=255)                                                                                                                                                                                                                      ggCTTAACGTGCCCAAGTTGTCCGGCAAATTCATGGGATCGTCAAATTGGGCTTCCAAACAGACTGGCGAAATATCCGTAAATACCGGCGTGAGAAACAATCGGTTTTGATAACGGCGAAGGATCTTATCCTCCAAACGAAACTGCGGCTGCGCATCGCTTTTGGCGAAAATGACATCGGAAATGAGCTGCTCCAGtt                                                      >  1:517425/1‑198 (MQ=255)                                                                                                                                                                                                                      ggCTTAACGTGCCCAAGTTGTCCGGCAAATTCATGGGATCGTCAAATTGGGCTTCCAAACAGACTGGCGAAATATCCGTAAATACCGGCGTGAGAAACAATCGGTTTTGATAACGGCGAAGGATCTTATCCTCCAAACGAAACTGCGGCTGCGCATCGCTTTTGGCGAAAATGACATCGGAAATGAGCTGCTCCAGTTGTGCCAATGAGGGCATCATCACACCCTGTTCCTGCAACCATAAACGCAGCaa  >  1:782560/1‑250 (MQ=255)                                                                                                                                                                                                                      ggCTTAACGTGCCCAAGTTGTCCGGCAAATTCATGGGATCGTCAAATTGGGCTTCCAAACAGACTGGCGAAATATCCGTAAATACCGGCGTGAGAAACAATCGGTTTTGATAACGGCGAAGGATCTTATCCTCCAAACGAAACTGCGGCTGCGCATCGCTTTTGGCGAAAATGACATCGGAAATGAGCTGCTCCAGTTGTGCCAATGAGGGCATCATCACACCCTGTTCCTGCAACCATAAACGCAGCaa  >  1:14173/1‑250 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                   GAATCCGTTGCCGCTGCCAAGGTGGCACGTTCAAACCTTGCCATACTTTTTTTATGTCCTGATTCACGCCTTGTTGTGTTAATTTAACTTTGCCCGAATAACCGAAACGGATCCAAACTTTCGTTCCCGCTAACGGTAAACCGAGGGTTTCTTTATATTCCTGCCCATTTTCATCGCGCCAAAGTGCGGTCATTTTTTCCAGTGTTTTTTTCAGGCTTAACGTGCCCAAGTTGTCCAGCAAATTCATGGGATCGTCAAATTGGGCTTCCAAACAGACTGGCGAAATATCCGTAAATACCGGCGTGAGAAACAATCGGTTTTGATAACGGCGAAGGATCTTATCCTCCAAACGAAACTGCGGCTGCGCATCGCTTTTGGCGAAAATGACATCGGAAATGAGCTGCTCCAGTTGTGCCAATGAGGGCATCATCACACCCTGTTCCTGCAACCATAAACGCAGCAA  >  1/1121197‑1121659

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑