Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,120,733	G→A	intergenic (+5243/‑18321)	unknown → / → unknown	tRNA‑Gln‑TTG/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,120,733	0	G	A	100.0%	160.0 / NA	54	intergenic (+5243/‑18321)	unknown/unknown	tRNA‑Gln‑TTG/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/26);  total (28/26)

GCGATTTTACCGGTGGTGTTCGCCCTTGTGATGACGGCGGTATTCGATGCTACAGGCACCATTCGCGCTGTTGCCGGACAAGCGAATTTATTGGACAAAGACGGTCAAATCATCAATGGCGGTAAAGCCTTAACCTCCGATTCCGTGAGCAGTTTATTCTCTGGTATTTTCGGCACAGCGCCGGCAGCGGTTTACATTGAATCCGCCGCAGGCACCGCAGCGGGCGGCAAAACCGGTGTCACCGCTATTGTGGTCGGCGTGTTGTTCCTATTAATGCTGTTCTTCCAACCGCTTGCTTTCTTAGTGCCGAATTACGCCACTGCGCCGGCATTAATGTATGTGGGGTTATTAATGTTGAGCAACGTGAGCAAATTAGATTTTGACGATTTCGTCGGCGCCATGAGCGGCTTGATTTGTGCGGTATTCATCGTGTTAACCGCCAACATCGTTACTGGTATTATGCTC  >  1/1120496‑1120960                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                    gCGATTTTACCGGTGGTGTTCGCCCTTGTGATGACGGCGGTATTCGATGCTACAGGCACCATTCGCGCTGTTGCCGGACAAGCGAATTTATTGGACAAAGACGGTCAAATCATCAATGGCGGTAAAGCCTTAACCTCCGATTCCGTGAGCAGTTTATTCTCTGGTATTTTCGGCACAGCGCCGGCAGCGGTTTACATTGAATCCGCCGCAGGCACCGCAGCGGGCGGCAAAACCGGTATCACCGCt                                                                                                                                                                                                                             >  1:404040/1‑246 (MQ=255)     ttttACCGGTGGTGTTCGCCCTTGTGATGACGGCGGTATTCGATGCTACAGGCACCATTCGCGCTGTTGCCGGACAAGCGAATTTATTGGACAAAGACGGTCAAATCATCAATGGCGGTAAAGCCTTAACCTCCGATTCCGTGAGCAGTTTATTCTCTGGTATTTTCGGCACAGCGCCGGCAGCGGTTTACATTGAATCCGCCGCAGGCACCGCAGCGGGCGGCAAAACCGGTATCACCGCTATTGTGGTc                                                                                                                                                                                                                    <  1:120511/251‑1 (MQ=255)                gtgtTCGCCCTTGTGATGACGGCGGTATTCGATGCTACAGGCACCATTCGCGCTGTTGCCGGACAAGCGAATTTATTGGACAAAGACGGTCAAATCATCAATGGCGGTAAAGCCTTAACCTCCGATTCCGTGAGCAGTTTATTCTCTGGTATTTTCGGCACAGCGCCGGCAGCGGTTTACATTGAATCCGCCGCAGGCACCGCAGCGGGCGGCAAAACCGGTATCACCGCTATTGTGGTCGGCGTgttgtt                                                                                                                                                                                                         >  1:364593/1‑251 (MQ=255)                 tgtTCGCCCTTGTGATGACGGCGGTATTCGATGCTACAGGCACCATTCGCGCTGTTGCCGGACAAGCGAATTTATTGGACAAAGACGGTCAAATCATCAATGGCGGTAAAGCCTTAACCTCCGATTCCGTGAGCAGTTTATTCTCTGGTATTTTCGGCACAGCGCCGGCAGCGGTTTACATTGAATCCGCCGCAGGCACCGCAGCGGGCGGCAAAACCGGTATCACCGCTATTGTGGTCGGCGTGTTGTTc                                                                                                                                                                                                        <  1:351172/251‑1 (MQ=255)                  gtTCGCCCTTGTGATGACGGCGGTATTCGATGCTACAGGCACCATTCGCGCTGTTGCCGGACAAGCGAATTTATTGGACAAAGACGGTCAAATCATCAATGGCGGTAAAGCCTTAACCTCCGATTCCGTGAGCAGTTTATTCTCTGGTATTTTCGGCACAGCGCCGGCAGCGGTTTACATTGAATCCGCCGCAGGCACCGCAGCGGGCGGCAAAACCGGTATCACCGCTATtg                                                                                                                                                                                                                         <  1:331500/233‑1 (MQ=255)                       cccTTGTGATGACGGCGGTATTCGATGCTACAGGCACCATTCGCGCTGTTGCCGGACAAGCGAATTTATTGGACAAAGACGGTCAAATCATCAATGGCGGTAAAGCCTTAACCTCCGATTCCGTGAGCAGTTTATTCTCTGGTATTTTCGGCACAGCGCCGGCAGCGGTTTACATTGAATCCGCCGCAGGCACCGCAGCGGGCGGCAAAACCGGTATCACCGCTATTGTGGGCGGCGTGTTGTTCCTATTa                                                                                                                                                                                                  >  1:751110/1‑251 (MQ=255)                       cccTTGTGATGACGGCGGTATTCGATGCTACAGGCACCATTCGCGCTGTTGCCGGACAAGCGAATTTATTGGACAAAGACGGTCAAATCATCAATGGCGGTAAAGCCTTAACCTCCGATTCCGTGAGCAGGTTATTCTCTGGTATTTTCGGCACAGCGCCGGCAGCGGTTTACATTGAATCCGCCGCAGGCACCGCAGCGGGCGGCAAAACCGGGATCACCGCTATTGTGGTCGGCGTGTTGTTCCTATTa                                                                                                                                                                                                  >  1:413068/1‑251 (MQ=255)                         ctggtgATGACGGTGGTATTCGATGCTACAGGCACCTTTCGCGCTGTTGCCGGCCAAGCGAATTTATTGGACAAAGACGGTCAAATCATCAATGGCGGTAAAGCCTTATCCTCCGATTCCGTGTGCAGTTTATTCTCTGGTATTTTCGGCACAGGTCCGGCAGCGGTTTACATTTAATCCGCCGCAGGCACCGCAGCGGGCGGCAAAACCGGTATCTCCGCTAGTGTGGTCGGCGTGTTGTTCCTATTAAt                                                                                                                                                                                                <  1:577826/248‑1 (MQ=255)                              gatgaCGGCGGTATTCGATGCTACAGGCACCATTCGCGCTGTTGCCGGACAAGCGAACTTATTGGACAAAGACGGTCAAATCATCAATAGCGGTAAAGCCTTAACCTCCGATTCCGTGAGCAGTTTATTCTCTGGTATTTTCGGCACAGCGCCGGCAGCGGTTTACATTGAATCCGCCGCAGGCACCGCAGCGGGCGGCAAAACCGGTATCACCGCTATTGTGGTCGGCGTGTTGTTCCTATTAATg                                                                                                                                                                                               >  1:679205/1‑247 (MQ=255)                                           ttCGATGCTACAGGCACCATTCGCGCTGTTGCCGGACAAGCGAATTTATTGGAAAAAGACGGTCAAATCATCAATGGCGGTAAAGCCTTAACCTCCGATTCCGTGAGCAGTTTATGCTCTGGTATTTTCGGCACAGCGCGGGCAGCGGTTTACATTGAATCCGCCGCAGGCACCGCAGCGGGCGGCAAAACCGGTATCACCGCTATTGTGGTCGGCGTGTTGTTCCTATTAATGc                                                                                                                                                                                              <  1:120327/235‑1 (MQ=255)                                                 gCTACAGGCACCATTCGCGCTGTTGCCGGACAAGCGAATTTATTGGACAAAGACGGTCAAATCATCAATGGCGGTAAAGCCTTAACCTCCGATTCCGTGAGCAGTTTATTCTCTGGTATTTTCGGCACAGCGCCGGCAGCGGTTTACATTGAATCCGCCGCAGGCACCGCAGCGGGCGGCAAAACCGGTATCACCGCTATTGTGGTCGGCGTGTTGTTCCTATTAATGCTGTTCTTCCAACCGCTTGCttt                                                                                                                                                                        >  1:392868/1‑251 (MQ=255)                                                  cTACAGGCACCATTCGCGCTGTTGCCGGACAAGCGAATTTATTGGACAAAGACGGTCAAATCATCAATGGCGGTAAAGCCTTAACCTCCGATTCCGGGAGCAGTTTATTCTCTGGTA‑TTTCGGCACAGCGCCGGCAGCGGGTTACATTGAATCCGCCGCAGGCACCGCAGCGGGCGGCAAAACCGGTATCACCGCTATTGTGGTCGGCGTGTTGTTCCTATTAATGCTGTTCTTCCAACCGCTTGCttt                                                                                                                                                                        >  1:95626/1‑249 (MQ=255)                                                       ggCACCATTCGCGCTGTTGCCGGACAAGCGAATTTATTGGACAAAGACGGTCAAATCATCAATGGCGGTAAAGCCTTAACCTCCGATTCCGTGAGCAGTTTATTCTCTGGTATTTTCGGCACAGCGCCGGCAGCGGTTTACATTGAATCCGCCGCAGGCACCGCAGCGGGCGGCAAAACCGGTATCACCGCTATtg                                                                                                                                                                                                                         >  1:121946/1‑196 (MQ=255)                                                       ggCACCATTCGCGCTGTTGCCGGACAAGCGAATTTATTGGACAAAGACGGTCAAATCATCAATGGCGGTAAAGCCTTAACCTCCGATTCCGTGAGCAGTTTATTCTCTGGTATTTTCGGCACAGCGCCGGCAGCGGTTTACATTGAATCCGCCGCAGGCACCGCAGCGGGCGGCAAAACCGGTATCACCGCTATTGTGGTCGGCGTGTTGTTCCTATTAATGCTGTTCTTCCAACCGCTTGCTTTCTTAGt                                                                                                                                                                  >  1:837879/1‑251 (MQ=255)                                                                cgcgCTGTTGCCGGACAAGCGAATTTATTGGACAAAGACGGTCAAATCATCAATGGCGGTAAAGCCTTAACCTCCGATTCCGTGAGCAGTTTATTCTCTGGTATTTTCGGCACAGCGCCGGCAGCGGTTTACATTGAATCCGCCGCAGGCACCGCAGCGGGCGGCAAAACCGGTATCACCGCTATTGTGGTCGGCGTGTTGTTCCTATTAATGCTGTTCTTCCAACCGCTTGCTTTCTTAGTGCCGAATTa                                                                                                                                                         <  1:278360/251‑1 (MQ=255)                                                                    cTGTTGCCGGACAAGCGAATTTATTGGACAAAGACGGTCAAATCATCAATGGCGGTAAAGCCTTAACCTCCGATTCCGTGAGCAGTTTATTCTCTGGTATTTTCGGCACAGCGCCGGCAGCGGTTTACATTGAATCCGCCGCAGGCACCGCAGCGGGCGGCAAAACCGGTATCACCGCTATTGTGGTCGGCGTGTTGTTCCTATTAATGCTGTTCTTCCAACCGCTTGCTTTCTTAGTGCCGAATTACGcc                                                                                                                                                     <  1:487053/251‑1 (MQ=255)                                                                      gTTGCCGGACAAGCGAATTTATTGGACAAAGACGGTCAAATCATCAATGGCGGTAAAGCCTTAACCTCCGATTCCGTGAGCAGTTTATTCTCTGGTATTTTCGGCACAGCGCCGGCAGCGGTTTACATTGAATCCGCCGCAGGCACCGCAGCGGGCGGCAAAACCGGTATCACCGCTATTGTGGTCGGCGTGTTGTTCCTATTAATGCTGTTCTTCCAACCGCTTGCTTTCTTAGTGCCGAATTACGCCa                                                                                                                                                    >  1:569224/1‑250 (MQ=255)                                                                          ccGGACAAGCGAATTTATTGGACAAAGACGGTCAAATCATCAATGGCGGTAAAGCCTTAACCTCCGATTCCGTGAGCAGTTTATTCTCTGGTATTTTCGGCACAGCGCCGGCAGCGGTTTACATTGAATCCGCCGCAGGCACCGCAGCGGGCGGCAAAACCGGTATCACCGCTATTGTGGTCGGCGTGTTGTTCCTATTAATGCTGTTCTTCCAACCGCTTGCTTTCTTAGTGCCGAATTACGCCACTgc                                                                                                                                                <  1:397759/250‑1 (MQ=255)                                                                            ggACAAGCGAATTTATTGGACAAAGACGGTCAAATCATCAATGGCGGTAAAGCCTTAACCTCCGATTCCGTGAGCAGTTTATTCTCTGGTATTTTCGGCACAGCGCCGGCAGCGGTTTACATTGAATCCGCCGCAGGCACCGCAGCGGGCGGCAAAACCGGTATCACCGCTATTGTGGTCGGCGTGTTGTTCCTATTAATGCTGTTCTTCCAACCGCTTGCTTTCTTAGTGCCGAATTACGCCACTgcgc                                                                                                                                              >  1:458666/1‑250 (MQ=255)                                                                                      aTTTATTGGACAAAGACGGTCAAATCATCAATGGCGGTAAAGCCTTAACCTCCGATTCCGTGAGCAGTTTATTCTCTGGTATTTTCGGCCCAGCGCCGGCAGCGGTTTACATTGAATCCGCCGCAGGCACCGCAGCGGGCGGCAAAACCGGTATCACCGCTATTGTGGTCGGCGTGTTGTTCCTATTAATGCTGTTCTTCCAACCGCTTGCTTTCTTAGTGCCGAATTACGCCACTGCGCCGGCATTAatg                                                                                                                                   <  1:319448/251‑1 (MQ=255)                                                                                      aTTTATTGGACAAAGACGGTCAAATCATCAATGGCGGTAAAGCCTTAACCTCCGATTCCGTGAGCAGTTTATTCTCTGGTATTTTCGGCACAGCGCCGGCAGCGGTTTACATTGAATCCGCCGCAGGCACCGCAGCGGGCGGCAAAACCGGTATCACCGCTATTGTGGTCGGCGTGTTGTTCCTATTAATGCTGTTCTTCCAACCGCTTGCTTTCTTAGTGCCGAATTACGCCACTGCGCCGGCATTAatg                                                                                                                                   <  1:650922/251‑1 (MQ=255)                                                                                        ttATTGGACAAAGACGGTCAAATCATCAATGGCGGTAAAGCCTTAACCTCCGATTCCGTGAGCAGTTTATTCTCTGGTATTTTCGGCACAGCGCCGGCAGCGGTTTACATTGAATCCGCCGCAGGCACCGCAGCGGGCGGCAAAACCGGTATCACCGCTATTGTGGTCGGCGTGTTGTTCCTAtt                                                                                                                                                                                                   <  1:855747/185‑1 (MQ=255)                                                                                         tATTGGACAAAGACGGTCAAATCATCAATGGCGGTAAAGCCTTAACCTCCGATTCCGTGAGCAGTTTATTCTCTGGTATTTTCGGCACAGCGCCGGCAGCGGTTTACATTGAATCCGCCGCAGGCACCGCAGCGGGCGGCAAAACCGGTATCACCGCTATTGTGGTCGGCGTGTTGTTCCTATTAATGCTGTTCTTCCAACCGCTTGCTTTCTTAGTGCCGAATTACGCCACTGCGCCGGCATTAatgtat                                                                                                                                <  1:326055/251‑1 (MQ=255)                                                                                          aTTGGACAAAGACGGTCAAATCATCAATGGCGGTAAAGCCTTAACCTCCGATTCCGTGAGCAGTTTATTCTCTGGTATTTTCGGCACAGCGCCGGCAGCGGTTTACATTGAATCCGCCGCAGGCACCGCAGCGGGCGGCAAAACCGGTATCACCGCTATTGTGGTCGGCGTGTTGTTCCTATTAATGCTGTTCTTCCAACCGCTTGCTTTCTTAGTGCCGAATTACGCCACTGCGCCGGCATTAatgtatg                                                                                                                               <  1:877109/251‑1 (MQ=255)                                                                                          aTTGGACAAAGACGGTCAAATCATCAATGGCGGTAAAGCCTTAACCTCCGATTCCGTGAGCAGTTTATTCTCTGGTATTTTCGGCACAGCGCCGGCAGCGGTTTACATTGAATCCGCCGCAGGCACCGCAGCGGGCGGCAAAACCGGTATCACCGCTATTGTGGTCGGCGTGTTGTTCCTATTAATGCTGTTCTTCCAACCGCTTGCTTTCTTAGTGCCGAATTACGCCACTGCGCCGGCATTAatgtatg                                                                                                                               <  1:774398/251‑1 (MQ=255)                                                                                            tGGACAAAGACGGTCAAATCATCAATGGCGGTAAAGCCTTAACCTCCGATTCCGTGAGCAGTTTATTCTCTGGTATTTTCGGCACAGCGCCGGCAGCGGTTTACATTGAATCCGCCGCAGGCACCGCAGCGGGCGGCAAAACCGGTATCACCGCTATTGTGGTCGGCGTGTTGTTCCTATTAATGCTGTTCTTCCAACCGCTTGCTTTCTTAGTGCCGAATTACGCCACTGCGCCGGCATTAATGTAtgtg                                                                                                                             <  1:357406/251‑1 (MQ=255)                                                                                             ggACAAAGACGGTCAAATCATCAATGGCGGTAAAGCCTTAACCTCCGATTCCGTGAGCAGTTTATTCTCTGGTATTTTCGGCACAGCGCCGGCAGCGGTTTACATTGAATCCGCCGCAGGCACCGCAGCGGGCGGCAAAACCGGTATCACCGCTATTGTGGTCGGCGTGTTGTTCCTATTAATGCTGTTCTTCCAACCGCTTGCTTTCTTAGTGCCGAATTACGCCACTGCGCCGGCATTAATGTATGTgg                                                                                                                            >  1:718015/1‑251 (MQ=255)                                                                                             ggACAAAGACGGTCAAATCATCAATGGCGGTAAAGCCTTAACCTCCGATTCCGTGAGCAGTTTATTCTCTGGTATTTTCGGCACAGCGCCGGCAGCGGTTTACATTGAATCCGCCGCAGGCACCGCAGCGGGCGGCAAAACCGGTATCACCGCTATTGTGGTCGGCGTGTTGTTCCTATTAATGCTGTTCTTCCAACCGCTTGCTTTCTTAGTGCCGAATTACGCCACTGCGCCGGCATTAATGTATGTgg                                                                                                                            >  1:760903/1‑251 (MQ=255)                                                                                                     aCGGTCAAATCATCAATGGCGGTAAAGCCTTAACCTCCGATTCCGTGAGCAGTTTATTCTCTGGTATTTTCGGCACAGCGCCGGCAGCGGTTTACATTGAATCCGCCGCAGGCACCGCAGCGGGCGGTAAAACCGGTATCACCGCTATTGTGGTCGGCGTGTTGTTCCTATTAATGCTGTTCTTCCAACCGCTTGCTTTCTTAGTGCCGAATTACGCCACTGCGCCGGCATTAATGTATGTGGGGttatt                                                                                                                     <  1:749250/250‑1 (MQ=255)                                                                                                      cGGTCAAATCATCAATGGCGGTAAAGCCTTAACCTCCGATTCCGTGAGCAGTTTATTCTCTGGTATTTTCGGCACAGCGCCGGCAGCGGTTTACATTGAATCCGCCGCAGGCACCGCAGCGGGCGGCAAAACCGGTATCACCGCTATTGTGGTCGGCGTGTTGTTCCTATTAATGCTGTTCTTCCAACCGCTTGCTTTCTTAGTGCCGAATTACGCCACTGCGCCGGCATTAATGTATGTGGGGTTATTaa                                                                                                                   >  1:748056/1‑251 (MQ=255)                                                                                                         tCAAATCATCAATGGCGGTAAAGCCTTAACCTCCGATTCCGTGAGCAGTTTATTCTCTGGTATTTTCGGCACAGCGCCGGCAGCGGTTTACATTGAATCCGCCGCAGGCACCGCAGCGGGCGGCAAAACCGGTATCACCGCTATTGTGGTCGGCGTGTTGTTCCTATTAATGCTGTTCTTCCAACCGCTTGCTTTCTTAGTGCCGAATTACGCCACTGCGCCGGCATTAATGTATGTGGGGTTATTAATGt                                                                                                                <  1:212051/251‑1 (MQ=255)                                                                                                             atcatcAATGGCGGTAAAGCCTTAACCTCCGATTCCGTGAGCAGTTTATTCTCTGGTATTTTCGGCACAGCGCCGGCAGCGGTTTACATTGAATCCGCCGCAGGCACCGCAGCGGGCGGCAAAACCGGTATCACCGCTATTGTGGTCGGCGTGTTGTTCCTATTAATGCTGTTCTTCCAACCGCTTGCTTTCTTAGTGCCGAATTACGCCACTGCGCCGGCATTAATGTATGTGGGGTTATTAATGTTGAg                                                                                                            >  1:126158/1‑251 (MQ=255)                                                                                                                               gCCTTAACCTCCGATTCCGTGAGCAGTTTATTCTCTGGTATTTTCGGCACAGCGCCGGCAGCGGTTTACATTGAATCCGCCGCAGGCACCGCAGCGGGCGGCAAAACCGGTATCACCGCTATTGTGGTCGGCGTGTTGTTCCTATTAATGCTGTTCTTCCAACCGCTTGCTTTCTTAGTGCCGAATTACGCCACTGCGCCGGCATTAATGTATGTGGGGTTATTAATGt                                                                                                                <  1:667507/229‑1 (MQ=255)                                                                                                                                 cTTAACCTCCGATTCCGTGAGCAGTTTATTCTCTGGTATTTTCGGCACAGCGCCGGCAGCGGTTTACATTGAATCCGCCGCAGGCACCGCAGCGGGCGGCAAAACCGGTATCACCGCTATTGTGGTCGGCGTGTTGTTCCTATTAATGCTGTTCTTCCAACCGCTTGCTTTCTTAGTGCCGAATTACGCCACTGCGCCGGCATTAATGTATGTGGGGTTATTAATGTTGAGCAACGTGAGCAAATTAGAtt                                                                                        <  1:621515/251‑1 (MQ=255)                                                                                                                                      ccTCCGATTCCGTGAGCAGTTTATTCTCTGGTATTTTCGGCACAGCGCCGGCAGCGGTTTACATTGAATCCGCCGCAGGCACCGCAGCGGGCGGCAAAACCGGTATCACCGCTATTGTGGTCGGCGTGTTGTTCCTATTAATGCTGTTCTTCCAACCGCTTGCTTTCTTAGTGCCGAATTACGCCACTGCGCCGGCATTAATGTATGTGGGGTTATTAATGTTGAGCAACGTGAGCAAATTAGATTTTGa                                                                                    >  1:396102/1‑250 (MQ=255)                                                                                                                                       cTCCGATTCCGTGAGCAGTTTATTCTCTGGTATTTTCGGCACAGCGCCGGCAGCGGTTTACATTGAATCCGCCGCAGGCACCGCAGCGGGCGGCAAAACCGGTATCACCGCTATTGTGGTCGGCGTGTTGTTCCTATTAATGCTGTTCTTCCAACCGCTTGCTTTCTTAGTGCCGAATTACGCCACTGCGCCGGCATTAATGTATGTGGGGTTATTAATGTTGAGCAACGTGAGCAAATTAGATTTTGACg                                                                                  >  1:417892/1‑251 (MQ=255)                                                                                                                                              tCCGTGAGCAGTTTATTCTCTGGTATTTTCGGCACAGCGCCGGCAGCGGTTTACATTGAATCCGCCGCAGGCACCGCAGCGGGCGGCAAAACCGGTATCACCGCTATTGTGGTCGGCGTGTTGTTCCTATTAATGCTGTTCTTCCAACCGCTTGCTTTCTTAGTGCCGAATTACGCCACTGCGCCGGCATTAatg                                                                                                                                   >  1:127843/1‑195 (MQ=255)                                                                                                                                               ccGTGAGCAGTTTATTCTCTGGTATTTTCGGCACAGCGCCGGCAGCGGTTTACATTGAATCCGCCGCAGGCACCGCAGCGGGCGGCAAAACCGGTATCACCGCTATTGTGGTCGGCGTGTTGTTCCTATTAATGCTGTTCTTCCAACCGCTTGCTTTCTTAGTGCCGAATTACGCCACTGCGCCGGCATTAATGTATGTGGGGTTATTAATGTTGAGCAACGTGAGCAAATTAGATTTTGACGATTtcgt                                                                           >  1:521993/1‑250 (MQ=255)                                                                                                                                                      cAGTTTATTCTCTGGTATTTTCGGCACAGCGCCGGCAGCGGTTTACATTGAATCCGCCGCAGGCACCGCAGCGGGCGGCAAAACCGGTATCACCGCTATTGTGGTCGGCGTGTTGTTCCTATTAATGCTGTTCTTCCAACCGCTTGCTTTCTTAGTGCCGAATTACGCCACTGCGCCGGCATTAATGTATGTGGGGTTATTAATGTTGAGCAACGTGAGCAAATTAGATTTTGACGATTTCGTCGGCGCCa                                                                   >  1:464923/1‑251 (MQ=255)                                                                                                                                                        gTTTATTCTCTGGTATTTTCGGCACAGCGCCGGCAGCGGTTTACATTGAATCCGCCGCAGGCACCGCAGCGGGCGGCAAAACCGGTATCACCGCTATTGTGGTCGGCGTGTTGTTCCTATTAATGCTGTTCTTCCAACCGCTTGCTTTCTTAGTGCCGAATTACGCCACTGCGCCGGCATTAATGTATGTggg                                                                                                                           <  1:559051/193‑1 (MQ=255)                                                                                                                                                        gTTTATTCTCTGGTATTTTCGGCACAGCGCCGGCAGCGGTTTACATTGAATCCGCCGCAGGCACCGCAGCGGGCGGCAAAACCGGTATCACCGCTATTGTGGTCGGCGTGTTGTTCCTATTAATGCTGTTCTTCCAACCGCTTGCTTTCTTAGTGCCGAATTACGCCACTGCGCCGGCATTAATGTATGTGGGGTTATTAATGTTGAGCaa                                                                                                         >  1:203030/1‑211 (MQ=255)                                                                                                                                                        gTTTATTCTCTGGTATTTTCGGCACAGCGCCGGCAGCGGTTTACATTGAATCCGCCGCAGGCACCGCAGCGGGCGGCAAAACCGGTATCACCGCTATTGTGGTCGGCGTGTTGTTCCTATTAATGCTGTTCTTCCAACCGCTTGCTTTCTTAGTGCCGAATTACGCCACTGCGCCGGCATTAATGTATGTGGGGTTATTAATGTTGAGCAACGTGAGc                                                                                                  >  1:889833/1‑218 (MQ=255)                                                                                                                                                        gTTTATTCTCTGGTATTTTCGGCACAGCGCCGGCAGCGGTTTACATTGAATCCGCCGCAGGCACCGCAGCGGGCGGCAAAACCGGTATCACCGCTATTGTGGTCGGCGTGTTGTTCCTATTAATGCTGTTCTTCCAACCGCTTGCTTTCTTAGTGCCGAATTACGCCACTGCGCCGGCATTAATGTATGTGGGGTTATTAATGTTGAGCAACGTGAGCAAATTAGATTTTGACGATTTCGTCGGCGCCATg                                                                 >  1:728883/1‑251 (MQ=255)                                                                                                                                                        gTTTATTCTCTGGTATTTTCGGCACAGCGCCGGCAGCGGTTTACATTGAATCCGCCGCAGGCACCGCAGCGGGCGGCAAAACCGGTATCACCGCTATTGTGGTCGGCGTGTTGTTCCTATTAATGCTGTTCTTCCAACCGCTTGCTTTCTTAGTGCCGAATTACGCCACTGCGCCGGCATTAATGTATGTGGGGTTATTAATGTTGAGCAACGTGAGCAAATTAGATTTTGACGATTTCGTCGGCGCCATg                                                                 >  1:785061/1‑251 (MQ=255)                                                                                                                                                           tATTCTCTGGTATTTTCGGCACAGCGCCGGCAGCGGTTTACATTGAATCCGCCGCAGGCACCGCAGCGGGCGGCAAAACCGGTATCACCGCTATTGTGGTCGGCGTGTTGTTCCTATTAATGCTGTTCTTCCAACCGCTTGCTTTCTTAGTGCCGAATTACGCCACTGCGCCGGCATTAATGTATGTGGGGTTATTAATGTTGAGCAACGTGAGCAAATTAGATTTTGACGATTTCGTCGGCGCCATGAGc                                                              <  1:409609/251‑1 (MQ=255)                                                                                                                                                                     tATTTTCGGCACAGCGCCGGCAGCGGTTTACATTGAATCCGCCGCAGGCACCGCAGCGGGCGGCAAAACCGGTATCACCGCTATTGTGGTCGGCGTGTTGTTCCTATTAATGCTGTTCTTCCAACCGCTTGCTTTCTTAGTGCCGAATTACGCCACTGCGCCGGCATTAatg                                                                                                                                   >  1:862496/1‑172 (MQ=255)                                                                                                                                                                        tttCGGCACAGCGCCGGCAGCGGTTTACATTGAATCCGCCGCAGGCACCGCAGCGGGCGGCAAAACCGGTATCACCGCTATTGTGGTCGGCGTGTTGTTCCTATTAATGCTGTTCTTCCAACCGCTTGCTTTCTTAGTGCCGAATTACGCCACTGCGCCGGCATTAatg                                                                                                                                   >  1:9466/1‑169 (MQ=255)                                                                                                                                                                              cacaGCGCCGGCAGCGGTTTACATTGAATCCGCCGCAGGCACCGCAGCGGGCGGCAAAACCGGTATCACCGCTATTGTGGTCGGCGTGTTGTTCCTATTAATGCTGTTCTTCCAACCGCTTGCTTTCTTAGTGCCGAATTACGCCACTGCGCCGGCATTAATGTATGTGGGGTTATTAATGTTGAGCAACGTGAGCAAATTAGATTTTGACGATTTCGTCGGCGCCATGAGCGGCTTGATTTGTGCGGTAt                                           <  1:55687/251‑1 (MQ=255)                                                                                                                                                                                   cgcCGGCAGCGGTTTACATTGAATCCGCCGCAGGCACCGCAGCGGGCGGCAAAACCGGTATCACCGCTATTGTGGTCGGCGTGTTGTTCCTATTAATGCTGTTCTTCCAACCGCTTGCTTTCTTAGTGCCGAATTACGCCACTGCGCCGGCATTAATGTATGTGGGGTTATTAATGTTGAGCAACGTGAGCAAATTAGATTTTGACGATTTCGTCGGCGCCATGAGCGGCTTGATTTGTGCGGTATTCAt                                       >  1:799456/1‑250 (MQ=255)                                                                                                                                                                                   cgcCGGCAGCGGTTTACATTGAATCCGCCGCAGGCACCGCAGCGGGCGGCAAAACCGGTATCACCGCTATTGTGGTCGGCGTGTTGTTCCTATTAATGCTGTGCTTCCAACCGCTTGCTTTCTTAGTGCCGAATTACGCCACTGCGCCGGCATTAATGTATGTGGGGTTATTAATGTTGAGCAACGTGAGCAAATTAGATTTTGACGATTTCGTCGGCGCCATGAGCGGCTTGATTTGTGCGGTATTCATc                                      <  1:280416/251‑1 (MQ=255)                                                                                                                                                                                            cGGTTTACATTGAATCCGCCGCAGGCACCGCAGCGGGCGGCAAAACCGGTATCACCGCTATTGTGGTCGGCGTGTTGTTCCTATTAATGCTGTTCTTCCAACCGCTTGCTTTCTTAGTGCCGAATTACGCCACTGCGCCGGCATTAATGTATGTGGGGTTATTAATGTTAAGCAACGTGAGCAAATTAGATTTTGACGATTTCGTCGGCGCCATGAGCGGCTTGATTTGTGCGGTATTCATCGTGTTAAc                              >  1:443424/1‑250 (MQ=255)                                                                                                                                                                                              gTTTACATTGAATCCGCCGCAGGCACCGCAGCGGGCGGCAAAACCGGTATCACCGCTATTGTGGTCGGCGTGTTGTTCCTATTAATGCTGTTCTTCCAACCGCTTGCTTTCTTAGTGCCGAATTACGCCACTGCGCCGGCATTAATGTATGTGGGGTTATTAATGTTGAGCAACGTGAGCAAATTAGATTTTGACGATTTCGTCGGCGCCATGAGCGGCTTGATTTGTGCGGTATTCATCGTGTTAACCg                            <  1:870083/250‑1 (MQ=255)                                                                                                                                                                                                                       ccGCAGCGGGCGGCAAAACCGGTATCACCGCTATTGTGGTCGGCGTGTTGTTCCTATTAATGCTGTTCTTCCAACCGCTTGCTTTCTTAGTGCCGAATTACGCCACTGCGCCGGCATTAATGTATGTGGGGTTATTAATGTTGAGCAACGTGAGCAAATTAGATTTTGACGATTTCGTCGGCGCCATGAGCGGCTTGATTTGTGCGGTATTCATCGTGTTAACCGCCAACATCGTTACTGGTATTATGCTc  <  1:724176/251‑1 (MQ=255)                                                                                                                                                                                                                                        aCCGGTATCACCGCTATTGTGGTCGGCGTGTTGTTCCTATTAATGc                                                                                                                                                                                              <  1:646481/46‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                    GCGATTTTACCGGTGGTGTTCGCCCTTGTGATGACGGCGGTATTCGATGCTACAGGCACCATTCGCGCTGTTGCCGGACAAGCGAATTTATTGGACAAAGACGGTCAAATCATCAATGGCGGTAAAGCCTTAACCTCCGATTCCGTGAGCAGTTTATTCTCTGGTATTTTCGGCACAGCGCCGGCAGCGGTTTACATTGAATCCGCCGCAGGCACCGCAGCGGGCGGCAAAACCGGTGTCACCGCTATTGTGGTCGGCGTGTTGTTCCTATTAATGCTGTTCTTCCAACCGCTTGCTTTCTTAGTGCCGAATTACGCCACTGCGCCGGCATTAATGTATGTGGGGTTATTAATGTTGAGCAACGTGAGCAAATTAGATTTTGACGATTTCGTCGGCGCCATGAGCGGCTTGATTTGTGCGGTATTCATCGTGTTAACCGCCAACATCGTTACTGGTATTATGCTC  >  1/1120496‑1120960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑