Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,112,520	G→A	intergenic (‑187803/‑2588)	unknown ← / → unknown	tRNA‑Gly‑GCC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,112,520	0	G	A	100.0%	208.6 / NA	71	intergenic (‑187803/‑2588)	unknown/unknown	tRNA‑Gly‑GCC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (36/35);  total (36/35)

ATTTTTTATGACGATTTTAGTTCTGGGTATCAATCACAAAACTGCTTCCGTGGCATTGCGGGAAAAGGTGGCGTTTTCCGACGAAAAGCGCACTTTTGCTTTGCGTCACATTCAACAAACGCAGTTGGCGGAAAGTGCGGTGATTTTATCCACCTGTAATCGCACGGAAGTTTATCTGCACAATAAAAGCGTTCCGCCGCAAGAGACGCAAACCTGGATCACACTGGCGGTGCAGTGGTTTGCCGGCATTCATCAACTAGCGTTGGCGGAGCTGCAGCACTGTGTTTATACTCACGAAAATCTTCAGGCGGCGAATCATTTAATGGAAGTGGCGTGCGGTTTGGATTCGCTGATTTTAGGCGAACCGCAGATTTTGGGGCAGGTGAAGCAAGCCTACCACATGAGCGAGCAGCATTATCAACAGGAAGGGCAAACCATTTCCGGCGAACTATCCCGTTTATTCCAAAAAACCTTTGCTA  >  1/1112275‑1112753                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                          aTTTTTTATGACGATTTTAGTTCTGGGTATCAATCACAAAACTGCTTCCGTGGCATTGCGGGAAAAGGTGGCGTTTTCCGACGAAAAGCGCACTTTTGCTTTGCGTCACATTCAACAAACGCAGTTGGCGGAAAGTGCGGTGATTTTATCCACCTGTAATCGCACGGAAGTTTATCTGCACAATAAAAGCGTTCCGCCGCAAGAGACGCAAACCTGGATCACACTGGCGGTGCAGTGGTTTGCCGACAtt                                                                                                                                                                                                                                       >  1:255822/1‑250 (MQ=255)              aTTTTAGTTCTGGGTATCAATCACAAAACTGCTTCCGTGGCATTGCGGGAAAAGGTGGCGTTTTCCGACGAAAAGCGCACTTTTGCTTTGCGTCACATTCAACAAACGCAGTTGGCGGAAAGTGCGGTGATTTTATCCACCTGTAATCGCACGGAAGTTTATCTGCACAATAAAAGCGTTCCGCCGCAAGAGACGCAAACCTGGATCACACTGGCGGTGCAGTGGTTTGCCGACATTCATCAACTAGCGtt                                                                                                                                                                                                                         >  1:215739/1‑251 (MQ=255)               ttttAGTTCTGGGTATCAATCACAAAACTGCTTCCGTGGCATTGCGGGAAAAGGTGGCGTTTTCCGACGAAAAGCGCACTTTTGCTTTGCGTCACATTCAACAAACGCAGTTGGCGGAAAGTGCGGTGATTTTATCCACCTGTAATCGCACGGAAGTTTATCTGCACAATAAAAGCGTTCCGCCGCAAGAGACGCAAACCTGGATCACACTGGCGGTGCAGTGGTTTGCCGACATTCATCAACTAGCGTTg                                                                                                                                                                                                                        >  1:474612/1‑251 (MQ=255)                 ttAGTTCTGGGTATCAATCACAAAACTGCTTCCGTGGCATTGCGGGAAAAGGTGGCGTTTTCCGACGAAAAGCGCACTTTTGCTTTGCGTCACATTCAACAAACGCAGTTGGCGGAAAGTGCGGTGATTTTATCCACCTGTAATCGCACGGAAGTTTATCTGCACAATAAAAGCGTTCCGCCGCAAGAGACGCAAACCTGGATCACACTGGCGGTGCAGTGGTTTGCCGACATTCATCAACTAGCGTTgg                                                                                                                                                                                                                       >  1:556208/1‑250 (MQ=255)                    gTTCTGGGTATCAATCACAAAACTGCTTCCGTGGCATTGCGGGAAAAGGTGGCGTTTTCCGACGAAAAGCGCACTTTTGCTTTGCGTCACATTCAACAAACGCAGTTGGCGGAAAGTGCGGTGATTTTATCCACCTGTAATCGCACGGAAGTTTATCTGCACAATAAAAGCGTTCCGCCGCAAGAGACGCAAACCTGGATCACACTGGCGGTGCAGTGGTTTGCCGACATTCATCAACTAGCGTTGGc                                                                                                                                                                                                                      <  1:58899/248‑1 (MQ=255)                          ggTATCAATCACAAAACTGCTTCCGTGGCATTGCGGGAAAAGGTGGCGTTTTCCGACGAAAAGCGCACTTTTGCTTTGCGTCACATTCAACAAACGCAGTTGGCGGAAAGTGCGGTGATTTTATCCACCTGTAATCGCACGGAAGTTTATCTGCACAATAAAAGCGTTCCGCCGCAAGAGACGCAAACCTGGATCACACTGGCGGTGCAGTGGTTTGCCGACATTCATCAACTAGCGTTGGCGGAGCTgca                                                                                                                                                                                                             >  1:8587/1‑251 (MQ=255)                           gTATCAATCACAAAACTGCTTCCGTGGCATTGCGGGAAAAGGTGGCGTTTTCCGACGAAAAGCGCACTTTTGCTTTGCGTCACATTCAACAAACGCAGTTGGCGGAAAGTGCGGTGATTTTATCCACCTGTAATCGCACGGAAGTTTATCTGCACAATAAAAGCGTTCCGCCGCAAGAGACGCAAACCTGGATCACACTGGCGGTGCAGTGGGTTGCCGACATTCATCAACTAGCGTTGGCGGAGCTgcag                                                                                                                                                                                                            >  1:762870/1‑251 (MQ=255)                              tcaatcaCAAAACTGCTTCCGTGGCATTGCGGGAAAAGGTGGCGTTTTCCGACGAAAAGCGCACTTTTGCTTTGCGTCACATTCAACAAACGCAGTTGGCGGAAAGTGCGGTGATTTTATCCACCTGTAATCGCACGGAAGTTTATCTGCACAATAAAAGCGTTCCGCCGCAAGAGACGCAAACCTGGATCACACTGGCGGTGCAGTGGTTTGCCGACATTCATCAACTAGCGTTGGCGGAGCTGCAGCAc                                                                                                                                                                                                         <  1:338767/251‑1 (MQ=255)                                  tcaCAAAACTGCTTCCGTGGCATTGCGGGAAAAGGTGGCGTTTTCCGACGAAAAGCGCACTTTTGCTTTGCGTCACATTCAACAAACGCAGTTGGCGGAAAGTGCGGTGATTTTATCCACCTGTAATCGCACGGAAGTTTATCTGCACAATAAAAGCGTTCCGCCGCAAGAGACGCAAACCTGGATCACACTGGCGGTGCAGTGGTTTGCCGACATTCATCAACTAGCGTTGGCGGAGCTGCAGCACtgtg                                                                                                                                                                                                     >  1:238852/1‑251 (MQ=255)                                     caAAACTGCTTCCGTGGCATTGCGGGAAAAGGTGGCGTTTTCCGACGAAAAGCGCACTTTTGCTTTGCGTCACATTCAACAAACGCAGTTGGCGGAAAGTGCGGTGATTTTATCCACCTGTAATCGCACGGAAGTTTATCTGCACAATAAAAGCGTTCCGCCGCAAGAGACGCAAACCTGGATCACACTGGCGGTGCAGTGGTTTGCCGACATTCATCAACTAGCGTTGGCGGAGCTgcag                                                                                                                                                                                                            >  1:96194/1‑241 (MQ=255)                                      aaaaCTGCTTCCGTGGCATTGCGGGAAAAGGTGGCGTTTTCCGACGAAAAGCGCACTTTTGCTTTGCGTCACATTCAACAAACGCAGTTGGCGGAAAGTGCGGTGATTTTATCCACCTGTAATCGCACGGAAGTTTATCTGCACAATAAAAGCGTTCCGCCGCAAGAGACGCAAACCTGGATCACACTGGCGGTGCAGTGGTTTGCCGACATTCATCAACTAGCGTTGGCGGAGCTGCAGCACTGTGTTta                                                                                                                                                                                                 <  1:475420/251‑1 (MQ=255)                                         tcTGCTTCCGTGGCATTGCGGGAAAAGGTGGCGTTTTCCGACGAAAAGCGCACTTTTGCTTTGCGTCACATTCAACAAACGCAGTTGGCGGAAAGTGCGGTGATTTTATCCACCTGTAATCGCACGGAAGTTTATCTGCACAATAAAAGCGTTCCGCCGCAAGAGACGCAAACCTGGATCACACTGGCGGTGCAGTGGTTTGCCGACATTCATCAACTAGCGTTGGCGGAGCTGCAGCACTGTGTTTATAc                                                                                                                                                                                              <  1:32518/250‑1 (MQ=255)                                         aCTGCTTCCGTGGCATTGCGGGAAAAGGTGGCGTTTTCCGACGAAAAGCGCACTTTTGCTTTGCGTCACATTCAACAAACGCAGTTGGCGGAAAGTGCGGTGATTTTATCCACCTGTAATCGCACGGAAGTTTATCTGCACAATAAAAGCGTTCCGCCGCAAGAGACGCAAACCTGGATCACACTGGCGGTGCAGTGGTTTGCCGACATTCATCAACTAGCGTTGGCGGAGCTGCAGCACTGTGttt                                                                                                                                                                                                  <  1:201129/247‑1 (MQ=255)                                         aCTGCTTCCGTGGCATTGCGGGAAAAGGTGGCGTTTTCCGACGAAAAGCGCACTTTTGCTTTGCGTCACATTCAACAAACGCAGTTGGCGGAAAGTGCGGTGATTTTATCCACCTGTAATCGCACGGAAGTTTATCTGCACAATAAAAGCGTTCCGCCGCAAGAGACGCAAACCTGGATCACACTGGCGGTGCAGTGGTTTGCCGACATTCATCAACTAGCGTTGGCGGAGCTGCAGCACTGTGTTTATAc                                                                                                                                                                                              <  1:366740/251‑1 (MQ=255)                                         aCTGCTTCCGTGGCATTGCGGGAAAAGGTGGCGTTTTCCGACGAAAAGCGCACTTTTGCTTTGCGTCACATTCAACAAACGCAGTTGGCGGAAAGTGCGGTGATTTTATCCACCTGTAATCGCACGGAAGTTTATCTGCACAATAAAAGCGTTCCGCCGCAAGAGACGCAAACCTGGATCACACTGGCGGTGCAGTGGTTTGCCGACATTCATCAACTAGCGTTGGCGGAGCTGCAGCACTGTGTTTATAc                                                                                                                                                                                              <  1:175441/251‑1 (MQ=255)                                         aCTGCTTCCGTGGCATTGCGGGAAAAGGTGGCGTTTTCCGACGAAAAGCGCACTTTTGCTTTGCGTCACATTCAACAAACGCAGTTGGCGGAAAGTGCGGTGATTTTATCCACCTGTAATCGCACGGAAGTTTATCTGCACAATAAAAGCGTTCCGCCGCAAGAGACGCAAACCTGGATCACACTGGCGGTGCAGTGGTTTGCCGACATTCATCAACTAGCGTTGGCGGAGCTGCAGCACTGTGTTTATAc                                                                                                                                                                                              <  1:779084/251‑1 (MQ=255)                                         aCTGCTTCCGTGGCATTGCGGGAAAAGGTGGCGTTTTCCGACGAAAAGCGCACTTTTGCTTTGCGTCACATTCAACAAACGCAGTTGGCGGAAAGTGCGGTGATTTTATCCACCTGTAATCGCACGGAAGTTTATCTGCACAATAAAAGCGTTCCGCCGCAAGAGACGCAAACCTGGATCACACTGGCGGTGCAGTGGTTTGCCGACATTCATCAACTAGCGTTGGCGGAGCTGCAGCACTGTGTTTATAc                                                                                                                                                                                              <  1:337484/251‑1 (MQ=255)                                          cTGCTTCCGTGGCATTGCGGGAAAAGGTGGCGTTTTCCGACGAAAAGCGCACTTTTGCTTTGCGTCACATTCAACAAACGCAGTTGGCGGAAAGTGCGGTGATTTTATCCACCTGTAATCGCACGGAAGTTTATCTGCACAATAAAAGCGTTCCGCCGCAAGAGACGCAAACCTGGATCACACTGGCGGTGCAGTGGTTTGCCGACATTCATCAACTAGCGTTGGCGGAGCTGCAGCACTGTGTTTATACt                                                                                                                                                                                             <  1:727296/251‑1 (MQ=255)                                            gCTTCCGTGGCATTGCGGGAAAAGGTGGCGTTTTCCGACGAAAAGCGCACTTTTGCTTTGCGTCACATTCAACAAACGCAGTTGGCGGAAAGTGCGGTGATTTTATCCACCTGTAATCGCACGGAAGTTTATCTGCACAATAAAAGCGTTCCGCCGCAAGAGACGCAAACCTGGATCACACTGGCGGTGCAGTGGTTTGCCGACATTCATCAACTAGCGTTGGCGGAGCTGCAGCACTgtgt                                                                                                                                                                                                    <  1:74564/242‑1 (MQ=255)                                               tCCGTGGCATTGCGGGAAAAGGTGGCGTTTTCCGACGAAAAGCGCACTTTTGCTTTGCGTCACATTCAACAAACGCAGTTGGCGGAAAGTGCGGTGATTTTATCCACCTGTAATCGCACGGAAGTTTATCTGCACAATAAAAGCGTTCCGCCGCAAGAGACGCAAACCTGGATCACACTGGCGGTGCAGTGGTTTGCCGACATtcatca                                                                                                                                                                                                                                  >  1:557533/1‑209 (MQ=255)                                               tCCGTGGCATTGCGGGAAAAGGTGGCGTTTTCCGACGAAAAGCGCACTTTTGCTTTGCGTCACATTCAACAAACGCAGTTGGCGGAAAGTGCGGTGATTTTATCCACCTGTAATCGCACGGAAGTTTATCTGCACAATAAAAGCGTTCCGCCGCAAGAGACGCAAACCTGGATCACACTGGCGGCGCAGTGGCTTGCCGACATTCATCAACTAGCGTTCGCGGAGCTGCAGCACTGTGTTTATAccttt                                                                                                                                                                                          >  1:898963/1‑245 (MQ=255)                                                ccGTGGCATTGCGGGAAAAGGTGGCGTTTTCCGACGAAAAGCGCGCTTTTGCTTTGCGTCACAGTCAACAAACGCAGTTGGCGGAAAGTGCGGTGATTTTATCCACCTGTAATCGCACGGAAGTTTATCTGCACAATAAAAGCGTTCCGCCGCAAGAGACGCAAGCCTGGATCACACTGGCGGTGCAGTGGTTTGCCGACATTCATCAACTAGCGTTGGCGGAGCTGCAGCAc                                                                                                                                                                                                         <  1:433191/233‑1 (MQ=255)                                                      cATTGCGGGAAAAGGTGGCGTTTTCCGACGAAAAGCGCACTTTTGCTTTGCGTCACATTCAACAAACGCAGTTGGCGGAAAGTGCGGTGATTTTATCCACCTGTAATCGCACGGAAGTTTATCTGCACAATAAAAGCGTTCCGCCGCAAGAGACGCAAACCTGGATCACACTGGCGGTGCAGTGGTTTGCCGACATTCATCAACTAGCGTTGGCGGAGCTGCAGCACTGTGTTTATACTCACGAAAATCt                                                                                                                                                                                  <  1:332175/250‑1 (MQ=255)                                                                            ttCCGACGAAAAGCGCACTTTTGCTTTGCGTCACATTCAACAAACGCAGTTGGCGGAAAGTGCGGTGATTTTATCCACCTGTAATCGCACGGAAGTTTATCTGCACAATAAAAGCGTTCCGCCGCAAGAGACGCAAACCTGGATCACACTGGCGGTGCAGTGGTTTGCCGACATTCATCAACTAGCGTTGGCGGAGCTGCAGCACTGTGTTTATACTCACGAAAATCTTCAGGCGGCGAATCATTTAATgg                                                                                                                                                           <  1:648271/251‑1 (MQ=255)                                                                               cgacgaAAAGCGCACTTTTGCTTTGCGTCACATTCAACAAACGCAGTTGGCGGAAAGTGCGGTGATTTTATCCACCTGTAACCGCACGGAAGTTTATCTGCACAATAAAAGCGTTCCGCCGCAAGAGACGCAAACCTGGATCACACTGGCGGTGCAGTGGTTTGCCGACACTCATCAACTAGCGTTGGCGGAGCTGCAGCACTGTGTTTATACTCACGAAAATCTTCAGGCGGCGAATCATTTAATGGAAg                                                                                                                                                        <  1:438343/251‑1 (MQ=255)                                                                                      aaGCGCACTTTTGCTTTGCGTCACATTCAACAAACGCAGTTGGCGGAAAGTGCGGTGATTTTATCCACCTGTAATCGCACGGAAGTTTATCTGCACAATAAAAGCGTTCCGCCGCAAGAGACGCAAACCTGGATCACACTGGCGGTGCAGTGGTTTGCCGACATTCATCCACTAGCGTTGGCGGAGCTGCAGCACTGTGTTTATACTCACGAAAATCTTCAGGCGGCGAATCATTTAATGGAAGTGGCggg                                                                                                                                                 >  1:436469/1‑249 (MQ=255)                                                                                           cACTTTTGCTTTGCGTCACATTCAACAAACGCAGTTGGCGGAAAGTGCGGTGATTTTATCCACCTGTAATCGCACGGAAGTTTATCTGCACAATAAAAGCGTTCCGCCGCAAGAGACGCAAACCTGGATCACACTGGCGGTGCAGTGGTTTGCCGACATTCATCAACTAGCGTTGGCGGAGCTGCAGCACTGTGTTTATACTCACGAAAATCTTCAGGCGGCGAATCATTTAATGGAAGTGGCGTGCGGtt                                                                                                                                            >  1:275603/1‑251 (MQ=255)                                                                                             cTTTTGCTTTGCGTCACATTCAACAAACGCAGTTGGCGGAAAGTGCGGTGATTTTATCCACCTGTAATCGCACGGAAGTTTATCTGCACAATAAAAGCGTTCCGCCGCAAGAGACGCAAACCTGGATCACACTGGCGGTGCAGTGGTTTGCCGACATTCATCAACTAGCGTTGGCGGAGCTGCAGCACTGTGTTTATAc                                                                                                                                                                                              >  1:727284/1‑199 (MQ=255)                                                                                             cTTTTGCTTTGCGTCACATTCAACAAACGCAGTTGGCGGAAAGTGCGGTGATTTTATCCACCTGTAATCGCACGGAAGTTTATCTGCACAATAAAAGCGTTCCGCCGCAAGAGACGCAAACCTGGATCACACTGGCGGTGCAGTGGTTTGCCGACATTCATCAACTAGCGTTGGCGGAGCTGCAGCACTGTGTTTATACTCACGAAAATCTTCAGGCGGCGAATCATTTAATGGAAGTGGCGTGCgg                                                                                                                                              <  1:266157/247‑1 (MQ=255)                                                                                             cTTTTGCTTTGCGTCACATTCAACAAACGCAGTTGGCGGAAAGTGCGGTGATTTTATCCACCTGTAATCGCACGGAAGTTTATCTGCACAATAAAAGCGTTCCGCCGCAAGAGACGCAAACCTGGATCACACTGGCGGTGCAGTGGTTTGCCGACATTCATCAACTAGCGTTGGCGGAGCTGCAGCACTGTGTTTATACTCACGAAAATCTTCAGGCGGCGAATCATTTAATGGAAGTGGCGTGCGGTTTg                                                                                                                                          >  1:688863/1‑251 (MQ=255)                                                                                                  gctttgcGTCACATTCAACAAACGCAGTTGGCGGAAAGTGCGGTGATTTTATCCACCTGTAATCGCACGGAAGTTTATCTGCACAATAAAAGCGTTCCGCCGCAAGAGACGCAAACCTGGATCACACTGGCGGTGCAGTGGTTTGCCGACATTCATCAACTAGCGTTGGCGGAGCTGCAGCACTGTGTTTATACTCACGAAAATCTTCAGGCGGCGAATCATTTAATGGAAGTGGCGTGCGGTTTGGAtt                                                                                                                                      >  1:609784/1‑250 (MQ=255)                                                                                                         gTCACATTCAACAAACGCAGTTGGCGGAAAGTGCGGTGATTTTATCCACCTGTAATCGCACGGAAGTTTATCTGCACAATAAAAGCGTTCCGCCGCAAGAGACGCAAACCTGGATCACACTGGCGGTGCAGTGGTTTGCCGACATTCATCAACTAGCGTTGGCGGAGCTGCAGCACTGTGTTTATACTCACGAAAATCTTCAGGCGGCGAATCATTTAATGGAAGTGGCGTGCGGTTTGGAtt                                                                                                                                      >  1:247549/1‑243 (MQ=255)                                                                                                              aTTCAACAAACGCAGTTGGCGGAAAGTGCGGTGATTTTATCCACCTGTAATCGCGCGGAAGTTTATCTGCAAAATAAAAGCGTTCCGCCGCAAGAGACGCAAACCTGGATCACACTGGCGGTGCAGTGGTTTGCCGACATTCATCAACTAGCGTTGGCGGAGCTGCAGCACTGTGTTTATACTCACGAAAATCTTCAGGCGGCGAATCATTTAATGGAAGTGGCGTGCGGTTTGGATTCGCTGATTTTAgg                                                                                                                         <  1:247704/251‑1 (MQ=255)                                                                                                              aTTCAACAAACGCAGTTGGCGGAAAGTGCGGTGATTTTATCCACCTGTAATCGCACGGAAGTTTATCTGCACAATAAAAGCGTTCCGCCGCAAGAGACGCAAACCTGGATCACACTGGCGGTGCAGTGGTTTGCCGACATTCATCAACTAGCGTTGGCGGAGCTGCAGCACTGTGTTTATACTCACGAAAATCTTCAGGCGGCGAATCATTTAATGGAAGTGGCGTGCGGTTTGGATTCGCTGATTTTAgg                                                                                                                         <  1:469976/251‑1 (MQ=255)                                                                                                              aTTCAACAAACGCAGTTGGCGGAAAGTGCGGTGATTTTATCCACCTGTAATCGCACGGAAGTTTATCTGCACAATAAAAGCGTTCCGCCGCAAGAGACGCAAACCTGGATCACACTGGCGGTGCAGTGGTTTGCCGACATTCATCAACTAGCGTTGGCGGAGCTGCAGCACTGTGTTTATACTCACGAAAATCTTCAGGCGGCGAATCATTTAATGGAAGTGGCGTGCGGTTTGGATTCGCTGATTTTAgg                                                                                                                         <  1:477981/251‑1 (MQ=255)                                                                                                                 caacaaACGCAGTTGGCGGAAAGTGCGGTGATTTTATCCACCTGTAATCGCACGGAAGTTTATCTGCACAATAAAAGCGTTCCGCCGCAAGAGACGCAAACCTGGATCACACTGGCGGTGCAGTGGTTTGCCGACATTCATCAACTAGCGTTGGCGGAGCTGCAGCACTGTGTTTATACTCACGAAAATCTTCAGGCGGCGAATCATTTAATGGAAGTGGCGTGCGGGTTGGATTCGCTGAttt                                                                                                                             >  1:6374/1‑244 (MQ=255)                                                                                                                      aaCGCAGTTGGCGGAAAGTGCGGTGATTTTATCCACCTGTAATCGCACGGAAGTTTATCTGCACAATAAAAGCGTTCCGCCGCAAGAGACGCAAACCTGGATCACACTGGCGGTGCAGTGGTTTGCCGACATTCATCAACTAGCGTTGGCGGAGCTGCAGCACTGTGTTTATACTCACGAAAATCTTCAGGCGGCGAATCATTTAATGGAAGTGGCGTGCgg                                                                                                                                              <  1:82009/222‑1 (MQ=255)                                                                                                                       aCGCAGTTGGCGGAAAGTGCGGTGATTTTATCCACCTGTAATCGCACGGAAGTTTATCTGCACAATAAAAGCGTTCCGCCGCAAGAGACGCAAACCTGGATCACACTGGCGGTGCAGTGGTTTGCCGACATTCATCAACTAGCGTTGGCGGAGCTGCAGCACTGTGTTTATAc                                                                                                                                                                                              <  1:233092/173‑1 (MQ=255)                                                                                                                          cAGTTGGCGGAAAGTGCGGTGATTTTATCCACCTGTAATCGCACGGAAGTTTATCTGCACAATAAAAGCGTTCCGCCGCAAGAGACGCAAACCTGGATCACACTGGCGGTGCAGTGGTTTGCCGACATTCATCAACTAGCGTTGGCGGAGCTGCAGCACTGTGTTTATACTCACGAAAATCTTCAGGCGGCGAATCATTTAATGGAAGTGGCGTGCGGTTTGGATTCGCTGATTTTAGGCGAACCGCAGAt                                                                                                             >  1:673241/1‑251 (MQ=255)                                                                                                                             ttGGCGGAAAGTGCGGTGATTTTATCCACCTGTAATCGCACGGAAGTTTATCTGCACAATAAAAGCGTTCCGCCGCAAGAGACGCAAACCTGGATCACACTGGCGGTGCAGTGGTTTGCCGACATTCATCAACTAGCGTTGGCGGAGCTGCAGCACTGTGTTTATACTCACGAAAATCTTCAGGCGGCGAATCATTTAATGGAAGTGGCGTGCGGTTTGGATTCGCTGATTTTAGGCGAACCGCAGAtt                                                                                                            <  1:217986/249‑1 (MQ=255)                                                                                                                                    aaaGTGCGGTGATTTTATCCACCTGTAATCGCACGGAAGTTTATCTGCACAATAAAAGCGTTCCGCCGCAAGAGACGCAAACCTGGATCACACTGGCGGTGCAGTGGTTTGCCGACATTCATCAACTAGCGTTGGCGGAGCTGCAGCACTGTGTTTATACTCACGAAAATCTTCAGGCGGCGAATCATTTAATGGAAGTGGCGTGCGGTTTGGATTCGCTGATTTTAGGCGAACCGCAGATTTTGGGGCAg                                                                                                   >  1:587818/1‑251 (MQ=255)                                                                                                                                       gTGCGGTGATTTTATCCACCTGTAATCGCACGGAAGTTTATCTGCACAATAAAAGCGTTCCGCCGCAAGAGACGCAAACCTGGATCACACTGGCGGTGCAGTGGTTTGCCGACATTCATCAACTAGCGTTGGCGGAGCTGCAGCACTGTGTTTATACTCACGAAAATCTTCAGGCGGCGAATCATTTAATGGAAGTGGCGTGCGGGTTGGATTCGCTGATTTTAGGCGAACCGCAGATTTTGGGGCAgggg                                                                                                >  1:515446/1‑249 (MQ=255)                                                                                                                                            gTGATTTTATCCACCTGTAATCGCACGGAAGTTTATCTGCACAATAAAAGCGTTCCGCCGCAAGAGACGCAAACCTGGATCACACTGGCGGTGCAGTGGTTTGCCGACATTCATCAACTAGCGTTGGCGGAGCTGCAGCACTGTGTTTATACTCACGAAAATCTTCAGGCGGCGAATCATTTAATGGAAGTGGCGTGCGGTTTGGATTCGCTGATTTTAGGCGAACCGCAGATTTTGGGGCAGGTGaagc                                                                                            <  1:38961/250‑1 (MQ=255)                                                                                                                                              gATTTTATCCACCTGTAATCGCACGGAAGTTTATCTGCACAATAAAAGCGTTCCGCCGCAAGAGACGCAAACCTGGATCACACTGGCGGTGCAGTGGTTTGCCGACATTCATCAACTAGCGTTGGCGGAGCTGCAGCACTgtgt                                                                                                                                                                                                    >  1:768813/1‑144 (MQ=255)                                                                                                                                                     tCCACCTGTAATCGCACGGAAGTTTATCTGCACAATAAAAGCGTTCCGCCGCAAGAGACGCAAACCTGGATCACACTGGCGGTGCAGTGGTTTGCCGACATTCATCAACTAGCGTTGGCGGAGCTGCAGCACTGTGTTTATACTCACGAAAATCTTCAGGCGGCGAATCATTTAATGGAAGTGGCGTGCGGTTTGGATTCGCTGATTTTAGGCGAACCGCAGATTTTGGGGCAGGTGAAGCAAGCCTACca                                                                                  <  1:881983/251‑1 (MQ=255)                                                                                                                                                        aCCTGTAATCGCACGGAAGTTTATCTGCACAATAAAAGCGTTCCGCCGCAAGAGACGCAAACCTGGATCACACTGGCGGTGCAGTGGTTTGCCGACATTCATCAACTAGCGTTGGCGGAGCTGCAGCACTGTGTTTATACTCACGAAAATCTTCAGGCGGCGAATCATTTAATGGAAGTGGCGTGCGGTTTGGATTCGCTGATTTTAgg                                                                                                                         <  1:16962/209‑1 (MQ=255)                                                                                                                                                        aCCTGTAATCGCACGGAAGTTTATCTGCACAATAAAAGCGTTCCGCCGCAAGAGACGCAAACCTGGATCACACTGGCGGTGCAGAGGTTTGCCGACATTCATCAACTAGCGTTGGCGGAGCTGCAGTACTGTGTTTATACTCACGAAAATCTTCAGGCGGCGAATCATTTAATGGAAGTGGCGTGCGGTTTGGATTCGCTGAATTTAGGCGAACCGCAGATTTTTGGGGAGGTGAAGCAAGcccatcacc                                                                                >  1:257473/1‑243 (MQ=255)                                                                                                                                                         ccTGTAATCGCACGGAAGTTTATCTGCACAATAAAAGCGTTCCGCCGCAAGAGACGCAAACCTGGATCACACTGGCGGTGCAGTGGTTTGCCGACATTCATCAACTAGCGTTGGCGGAGCTGCAGCACTgtgt                                                                                                                                                                                                    >  1:306785/1‑133 (MQ=255)                                                                                                                                                         ccTGTAATCGCACGGAAGTTTATCTGCACAATAAAAGCGTTCCGCCGCAAGAGACGCAAACCTGGATCACACTGGCGGTGCAGTGGTTTGCCGACATTCATCAACTAGCGTTGGCGGAGCTGCAGCACTGTGTTTATACTCACGAAAATCTTCAGGCGGCGAATCATTTAAt                                                                                                                                                             >  1:292727/1‑172 (MQ=255)                                                                                                                                                         ccTGTAATCGCACGGAAGTTTATCTGCACAATAAAAGCGTTCCGCCGCAAGAGACGCAAACCTGGATCACACTGGCGGTGCAGTGGTTTGCCGACATTCATCAACTAGCGTTGGCGGAGCTGCAGCACTGTGTTTATACTCACGAAAATCTTCAGGCGGCGAATCATTTAATGGAAGTgg                                                                                                                                                     <  1:668080/180‑1 (MQ=255)                                                                                                                                                         ccTGTAATCGCACGGAAGTTTATCTGCACAATAAAAGCGTTCCGCCGCAAGAGACGCAAACCTGGATCACACTGGCGGTGCAGTGGTTTGCCGACATTCATCAACTAGCGTTGGCGGAGCTGCAGCACTGTGTTTATACTCACGAAAATCTTCAGGCGGCGAATCATTTAATGGAAGTGGCGTGCGGTTTGGATTCGCTGATTTTAGGCGAACCGCAGATTTTGGGGCAGGTGAAGCAAGCCTACCACATg                                                                              <  1:731890/251‑1 (MQ=255)                                                                                                                                                         ccTGTAATCGCACGGAAGTTTATCTGCACAATAAAAGCGTTCCGCCGCAAGAGACGCAAACCTGGATCACACTGGCGGTGCAGTGGTTTGCCGACATTCATCAACTAGCGTTGGCGGAGCTGCAGCACTGTGTTTATACTCACGAAAATCTTCAGGCGGCGAATCATTTAATGGAAGTGGCGTGCGGTTTGGATTCGCTGATTTTAGGCGAACCGCAGATTTTGGGGCAGGTGAAGCAAGCCTACCACATg                                                                              >  1:511968/1‑251 (MQ=255)                                                                                                                                                            gTAATCGCACGGAAGTTTATCTGCACAATAAAAGCGTTCCGCCGCAAGAGACGCAAACCTGGATCACACTGGCGGTGCAGTGGTTTGCCGACATTCATCAACTAGCGTTGGCGGAGCTGCAGCACTGTGTTTATACTCACGAAAATCTTCAGGCGGCGAATCATTTAATGGAAGTGGCGTGCGGTTTGGATTCGCTGATTTTAGGCGAACCGCAGATTTTGGGGCAGGTGAAGCAAGCCTACCACATgag                                                                            >  1:645265/1‑250 (MQ=255)                                                                                                                                                             tAATCGCACGGAAGTTTATCTGCACAATAAAAGCGTTCCGCCGCAAGAGACGCAAACCTGGATCACACTGGCGGTGCAGTGGTTTGCCGACATTCATCAACTAGCGTTGGCGGAGCTGCAGCACTGTGTTTATACTCACGAAAATCTTCAGGCGGCGAATCATTTAATGGAAGTGGCGTGCGGTTTGGATTCGCTGATTTTAGGCGAACCGCAGATTTTGGGGCAGGTGAAGCAAGCCTACCACATgagcg                                                                          <  1:366432/251‑1 (MQ=255)                                                                                                                                                                       gAAGTTTATCTGCACAATAAAAGCGTTCCGCCGCAAGAGACGCAAACCTGGATCACACTGGCGGTGCAGTGGTTTGCCGACATTCATCAACTAGCGTTGGCGGAGCTGCAGCACTGTGTTTATACTCACGAAAATCTTCAGGCGGCGAATCATTTAATGGAAGTGGCGTGCGGTTTGGATTCGCTGATTTTAGGCGAACCGCAGATTTTGGGGCAGGTGAAGCAAGCCTACCACATGAGCGAGCAGCATTa                                                                >  1:522717/1‑251 (MQ=255)                                                                                                                                                                          gTTTATCTGCACAATAAAAGCGTTCCGCCGCAAGAGACGCAAACCTGGATCACACTGGCGGTGCAGTGGTTTGCCGACATTCATCAACTAGCGTTGGCGGAGCTGCAGCACTGTGTTTATACTCACGAAAATCTTCAGGCGGCGAATCATTTAATGGAAGTGGCGTGCGGTTTGGATTCGCTGATTTTAGGCGAACCGCAGAtt                                                                                                            >  1:396776/1‑204 (MQ=255)                                                                                                                                                                             tATCTGCACAATAAAAGCGTTCCGCCGCAAGAGACGCAAACCCGGATCACACTGGCGGTGCAGTGGTTTGCCGACATTCATCAACTAGCGTTGGCGGAGCTGCAGCACTGTGTTTATACTCACGAAAATCTTCAGGCGGCGAATCATTTAATGGa                                                                                                                                                          >  1:565179/1‑155 (MQ=255)                                                                                                                                                                                     caATAAAAGCGTTCCGCCGCAAGAGACGCAAACCTGGATCACACTGGCGGTGCAGTGGTTTGCCGACATTCATCAACTAGCGTTGGCGGAGCTGCAGCACTGTGTTTATACTCACGAAAATCTTCAGGCGGCGAATCATTTAATGGAAGTGGCGTGCGGTTTGGATTCGCTGATTTTAGGCGAACCGCAGATTTTGGGGc                                                                                                     >  1:35335/1‑200 (MQ=255)                                                                                                                                                                                     caATAAAAGCGTTCCGCCGCAAGAGACGCAAACCTGGATCACACTGGCGGTGCAGTGGTTTGCCGACATTCATCAACTAACGTTGGCGGAGCTGCAGCACTGTGTTTATACTCACGAAAATCTTCAGGCGGCGAATCATTTAATGGAAGTGGCGTGCGGTTTGGATTCGCTGATTTTAGGCGAACCGCAGATTTTGGGGc                                                                                                     >  1:52852/1‑200 (MQ=255)                                                                                                                                                                                     caATAAAAGCGTTCCGCCGCAAGAGACGCAAACCTGGATCACACTGGCGGGGCAGTGGTTTGCCGACATTCATCAACTAGCGTTGGCGGAGCTGCAGCACTGTGTTTATACTCACGAAAATCTTCAGGCGGCGAATCATTTAATGGAAGTGGCGTGCGGTTTGGATTCGCTGATTTTAGGCGAACCGCAGATTTTGGGGCAGGTGAAGCAAGCCTACcac                                                                                 <  1:445134/220‑1 (MQ=255)                                                                                                                                                                                       aTAAAAGCGTTCCGCCGCAAGAGACGCAAACCTGGATCACACTGGCGGTGCAGTGGTTTGCCGACATTCATCAACTAGCGTTGGCGGAGCTGCAGCACTGTGTTTATACTCACGAAAATCTTCAGGCGGCGAATCATTTAATGGAAGTGGCGTGCGGTTTGGATTCGCTGATTTTAGGCGAACCGCAGATTTTGGGGCAGGGGAAGCAAGCCTACCACATGAGCGAGCAGCATTATCAACAGGAAGGGCaa                                                >  1:139103/1‑251 (MQ=255)                                                                                                                                                                                          aaaGCGTTCCGCCGCAAGAGACGCAAACCTGGATCACACTGGCGGTGCAGTGGTTTGCCGACATTCATCAACTAGCGTTGGCGGAGCTGCAGCACTGTGTTTATACTCACGAAAATCTTCAGGCGGCGAATCATTTAATGGAAGTGGCGTGCGGTTTGGATTCGCTGATTTTAGGCGAACCGCAGATTTTGGGGCAGGTGAAGCAAGCCTACCACATGAGCGAGCAGCATTATCAACAGGAAGGGCAAAcc                                             <  1:192648/251‑1 (MQ=255)                                                                                                                                                                                                ttCCGCCGCAAGAGACGCAAACCTGGATCACACTGGCGGTGCAGTGGTTTGCCGACATTCATCAACTAGCGTTGGCGGAGCTGCAGCACTGTGTTTATACTCACGAAAATCTTCAGGCGGCGAATCATTTAATGGAAGTGGCGTGCGGTTTGGATTCGCTGATTTTAGGCGAACCGCAGATTTTGGGGCAGGTGAAGCAAGCCTACCACATGAGCGAGCAGCATTATCAACAGGAAGGGCAAACCATTTc                                        <  1:273826/250‑1 (MQ=255)                                                                                                                                                                                                             gaCGCAAACCTGGATCACACTGGCGGTGCAGTGGTTTGCCGACATTCATCAACTAGCGTTGGCGGAGCTGCAGCACTGTGTTTATACTCACGAAAATCTTCAGGCGGCGAATCATTTAATGGAAGTGGCGTGCGGTTTGGATTCGCTGATTTTAGGCGAACCGCAGATTTTGGGGCAGGTGAAGCAAGCCTACCACATGAGCGAGCAGCATTATCAAAAGGAAGGGCAAACCATTTCCGGCGAACTATcc                           >  1:605023/1‑250 (MQ=255)                                                                                                                                                                                                                 cAAACCTGGATCACACTGGCGGTGCAGTGGTTTGCCGACATTCATCAACTAGCGTTGGCGGAGCTGCAGCACTGTGTTTATACTCACGAAAATCTTCAGGCGGCGAATCATTTAATGGAAGTGGCGTGCGGTTTGGATTCGCTGATTTTAGGCGAACCGCAGATTTTGGGGCAGGTGAAGCAAGCCTACca                                                                                  <  1:588804/191‑1 (MQ=255)                                                                                                                                                                                                                     ccTGGATCACACTGGCGGTGCAGTGGTTTGCCGACATTCATCAACTAGCGTTGGCGGAGCTGCAGCACTGTGTTTATACTCACGAAAATCTTCAGGCGGCGAATCATTTAATGGAAGTGGCGTGCGGGTTGGATTCGCTGATTTTAGGCGAACCGCAGATTTTGGGGCAGGGGAAGCAAGCCTACCACATGAGCGAGCAGCATTATCAACAGGAAGGGCAAACCATTTCCGGCGAACTATCCCGGTTAtt                   >  1:239646/1‑250 (MQ=255)                                                                                                                                                                                                                        ggATCACACTGGCGGTGCAGTGGTTTGCCGACATTCATCAACTAGCGTTGGCGGAGCTGCAGCACTGTGTTTATACTCACGAAAATCTTCAGGCGGCGAATCATTTAATGGAAGTGGCGTGCGGTTTGGATTCGCTGATTTTAGGCGAACCGCAGATTTTGGGGCAGGTGAAGCAAGCCTACCACATGAGCGAGCAGCATTATCAAc                                                           <  1:650299/207‑1 (MQ=255)                                                                                                                                                                                                                                      gTGCAGTGGTTTGCCGACATTCATCAACTAGCGTTGGCGGAGCTGCAGCACTGTGTTTATACTCACGAAAATCTTCAGGCGGCGAATCATTTAATGGAAGTGGCGTGCGGTTTGGATTCGCTGATTTTAGGCGAACCGCAGATTTTGGGGCAGGTGAAGCAAGCCTACCACATGAGCGAGCAGCATTATCAACAGGAAGGGCAAACCATTTCCGGCGAACTATCCCGTTTATTCCAAAAAACCTTTGCTa  <  1:610974/250‑1 (MQ=255)                                                                                                                                                                                                                                      gTGCAGTGGTTTGCCGACATTCATCAACTAGCGTTGGCGGAGCTGCAGCACTGTGTTTATACTCACGAAAATCTTCAGGCGGCGAATCATTTAATGGAAGTGGCGTGCGGTTTGGATTCGCTGATTTTAGGCGAACCGCAGATTTTGGGGCAGGTGAAGCAAGCCTACCACATGAGCGAGCAGCATTATCAACAGGAAGGGCAAACCATTTCCGGCGAACTATCCCGTTTATTCCAAAAAACCTTTGCTa  >  1:554930/1‑250 (MQ=255)                                                                                                                                                                                                                                      gTGCAGTGGTTTGCCGACATTCATCAACTAGCGTTGGCGGAGCTGCAGCACTGTGTTTATACTCACGAAAATCTTCAGGCGGCGAATCATTTAATGGAAGTGGCGTGCGGTTTGGATTCGCTGATTTTAGGCGAACCGCAGATTTTGGGGCAGGTGAAGCAAGCCTACCACATGAGCGAGCAGCATTATCAACAGGAAGGGCAAACCATTTCCGGCGAACTATCCCGTTTATTCCAAAAAACCTTTGCTa  >  1:279172/1‑250 (MQ=255)                                                                                                                                                                                                                                             ggTTTGCCGACATTCATCAACTAGCGTTGGCGGAGCTGCAGCACTGTGTTTATACTCACGAAAATCTTCAGGCGGCGAATCATTTAATGGAAGTGGCGTGCGGTTTGGATTCGCTGATTTTAGGCGAACCGCAGATTTTGGGGCAGGTGAAGCAAGCCTACCACATGAGCGAGCAGCATTATCAACAGGAAGGGCAAACCATTTCCGGCGAACTATcc                           <  1:774556/218‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                          ATTTTTTATGACGATTTTAGTTCTGGGTATCAATCACAAAACTGCTTCCGTGGCATTGCGGGAAAAGGTGGCGTTTTCCGACGAAAAGCGCACTTTTGCTTTGCGTCACATTCAACAAACGCAGTTGGCGGAAAGTGCGGTGATTTTATCCACCTGTAATCGCACGGAAGTTTATCTGCACAATAAAAGCGTTCCGCCGCAAGAGACGCAAACCTGGATCACACTGGCGGTGCAGTGGTTTGCCGGCATTCATCAACTAGCGTTGGCGGAGCTGCAGCACTGTGTTTATACTCACGAAAATCTTCAGGCGGCGAATCATTTAATGGAAGTGGCGTGCGGTTTGGATTCGCTGATTTTAGGCGAACCGCAGATTTTGGGGCAGGTGAAGCAAGCCTACCACATGAGCGAGCAGCATTATCAACAGGAAGGGCAAACCATTTCCGGCGAACTATCCCGTTTATTCCAAAAAACCTTTGCTA  >  1/1112275‑1112753

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑