Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,015,103	T→G	intergenic (‑90386/‑100005)	unknown ← / → unknown	tRNA‑Gly‑GCC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,015,103	0	T	G	100.0%	215.0 / NA	71	intergenic (‑90386/‑100005)	unknown/unknown	tRNA‑Gly‑GCC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (31/40);  total (31/40)

AAAGTGCGGTAGTTTTTCAACACGTTTTACACTTCTTATCATTTAACGGCTCCGCATAAATTTGCCGAACAGAGCGACTTTCACCGTGATAAAGTTGAATCGCCCCAACGCAATCCTGCTCAATAGCCGAAAGCAAATCAAAGGGGGATTTGGTCGAGGTCTGGAAACGTTGCTGAATGCGTGAACGAATTTGTTCATTTTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACTTTATGGGATAAAGGCAATGATAATGAAATTGGACGGGAGCGCGCAGAATCAAGCCATTTTGGGGGATATTGAAATTCCGTCGGCCATTTGTGAGCTTTCGCCATTCGCCGACCAGCAAGCCGTTCATTGCGACTTTCAAGATAGCGTTATTCATTACCACGCCTCATCGTCATCGGAAAATGCAGGTG  >  1/1014904‑1015342                                                                                                                                                                                                        |                                                                                                                                                                                                                                                aaaGTGCGGTAGTTTTTCAACACGTTTTACACTTCTTATCATTTAACGGCTCCGCATAAATTTGCCGAACAGAGCGACTTTCACCGTGATAAAGTTGAATCGCCCCAACGCAATCCTGCTCAATAGCCGAAAGCAAATCAAAGGGGGATTTGGTCGAGGTCTGGAAACGTTGCTGAATGCGTGAACGAATTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACt                                                                                                                                                                                              <  1:240237/251‑1 (MQ=255)       cGGTAGTTTTTCAACACGTTTTACACTTCTTATCATTTAACGGCTCCGCATAAATTTGCCGAACAGAGCGACTTTCACCGTGATAAAGTTGAATCGCCCCAACGCAATCCTGCTCAATAGCCGAAAGCAAATCAAAGGGGGATTTGGTCGAGGTCTGGAAACGTTGCTGAATGCGTGAACGAATTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACTTTATgg                                                                                                                                                                                        <  1:360384/251‑1 (MQ=255)           aGTTTTTCAACACGTTTTACACTTCTTATCATTTAACGGCTCCGCATAAATTTGCCGAACAGAGCGACTTTCACCGTGATAAAGTTGAATCGCCCCAACGCAATCCTGCTCAATAGCCGAAAGCAAATCAAAGGGGGATTTGGTCGAGGTCTGGAAACGTTGCTGAATGCGTGAACGAATTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACTTTATGGGATa                                                                                                                                                                                    >  1:182575/1‑251 (MQ=255)               tttCAACACGTTTTACACTTCTTATCATTTAACGGCTCCGCATAAATTTGCCGAACAGAGCGACTTTCACCGTGATAAAGTTGAATCGCCCCAACGCAATCCTGCTCAATAGCCGAAAGC‑AATCAAAGGGGGATTTGGTCGAGGTCTGGAAACGTTGCTGAATGCGTGAACGAATTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACTTTATGGGATAAAGGc                                                                                                                                                                               <  1:601424/251‑1 (MQ=255)                           ttACACTTCTTATCATTTAACGGCTCCGCATAAATTTGCCGAACAGAGCGACTTTCACCGTGATAAAGTTGAATCGCCCCAACGCAATCCTGCTCAATAGCCGAAAGCAAATCAAAGGGGGATTTGGTCGAGGTCTGGAAACGTTGCTGAATGCGTGAACGAATTTGTTCATTGTCGGGCAAAAGa                                                                                                                                                                                                                                     >  1:90759/1‑186 (MQ=255)                           ttACACTTCTTATCATTTAACGGCTCCGCATAAATTTGCCGAACAGAGCGACTTTCACCGTGATAAAGTTGAATCGCCCCAACGCAATCCTGCTCAATAGCCGAAAGCAAATCAAAGGGGGATTTGGTCGAGGTCTGGAAACGTTGCTGAATGCGTGAACGAATTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACt                                                                                                                                                                                              <  1:870313/225‑1 (MQ=255)                           ttACACTTCTTATCATTTAACGGCTCCGCATAAATTTGCCGAACAGAGCGACTTTCACCGTGATAAAGTTGAATCGCCCCAACGCAATCCTGCTCAATAGCCGAAAGCAAATCAAAGGGGGATTTGGTCGAGGTCTGGAAACGTTGCTGAATGCGTGAACGAATTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACTTTATGGGATAAAGGCAATGATAATGa                                                                                                                                                                    >  1:429860/1‑251 (MQ=255)                           ttACACTTCTTATCATTTAACGGCTCCGCATAAATTTGCCGAACAGAGCGACTTTCACCGTGATAAAGTTGAATCGCCCCAACGCAATCCTGCTCAATAGCCGAAAGCAAATCAAAGGGGGATTTGGTCGAGGTCTGGAAACGTTGCTGAATGCGTGAACGAATTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACTTTATGGGATAAAGGCAATGATAATGa                                                                                                                                                                    <  1:691243/251‑1 (MQ=255)                            tACACTTCTTATCATTTAACGGCTCCGCATAAATTTGCCGAACAGAGCGACTTTCACCGTGATAAAGTTGAATCGCCCCAACGCAATCCTGCTCAATAGCCGAAAGCAAATCAAAGGGGGATTTGGTCGAGGTCTGGAAACGTTGCTGAATGCGTGAACGAATTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACTTTATGGGATAAAGGCAATGATAATGaa                                                                                                                                                                   <  1:323002/251‑1 (MQ=255)                                cttcttATCATTTAACGGCTCCGCATAAATTTGCCGAACAGAGCGACTTTCACCGTGATAAAGTTGAATCGCCCCAACGCAATCCTGCTCAATAGCCGAAAGCAAATCAAAGGGGGATTTGGTCGAGGTCTGGAAACGTTGCTGAATGCGTGAACGAATTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACTTTATGGGATAAAgg                                                                                                                                                                                >  1:79693/1‑234 (MQ=255)                                cttcttATCATTTAACGGCTCCGCATAAATTTGCCGAACAGAGCGACTTTCACCGTGATAAAGTTGAATCGCCCCAACGCAATCCTGCTCAATAGCCGAAAGCAAATCAAAGGGGGATTTGGTCGAGGTCTGGAAACGTTGCTGAATGCGTGAACGAATTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACTTTATGGGATAAAGGCAATGATAATGAAATTg                                                                                                                                                               >  1:582525/1‑251 (MQ=255)                                       tCATTTAACGGCTCCGCATAAATTTGCCGAACAGAGCGACTTTCACCGTGATAAAGTTGAATCGCCCCAACGCAATCCTGCTCAATAGCCGAAAGCAAATCAAAGGGGGATTTGGTCGAGGTCTGGAAACGTTGCTGAATGCGTGAACGAATTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACTTTATGGGATAAAGGCAATGATAATGaaa                                                                                                                                                                  <  1:429840/241‑1 (MQ=255)                                       tCATTTAACGGCTCCGCATAAATTTGCCGAACAGAGCGACTTTCACCGTGATAAAGTTGAATCG‑CCCAACGCAATCCTGCTCAATAGCCGAAAGCAAATCAAAGGGGGATTTGGTCGAGGTCTGGAAACGTTGCTGAATGCGTGAACGAATTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACTTTATGGGATAAAGGCAATGATAATGAAATTGGACGGGAg                                                                                                                                                       <  1:374455/251‑1 (MQ=255)                                        cATTTAACGGCTCCGCATAAATTTGCCGAACAGAGCGACTTTCACCGTGATAAAGTTGAATCGCCCCAACGCAATCCTGCTCAATAGCCGAAAGCAAATCAAAGGGGGATTTGGTCGAGGTCTGGAAACGTTGCTGAATGCGTGAACGAATTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACTTTATGGGATAAAGGCAATGATAATGAAATTGGACGGGAg                                                                                                                                                       <  1:783795/251‑1 (MQ=255)                                          tttAACGGCTCCGCATAAATTTGCCGAACAGAGCGACTTTCACCGTGATAAAGTTGAATCGCCCCAACGCAATCCTGCTCAATAGCCGAAAGCAAATCAAAGGGGGATTTGGTCGAGGTCTGGAAACGTTGCTGAATGCGTGAACGAATTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACTTTATGGGATAAAGGc                                                                                                                                                                               >  1:679847/1‑225 (MQ=255)                                          tttAACGGCTCCGCATAAATTTGCCGAACAGAGCGACTTTCACCGTGATAAAGTTGAATCGCCCCAACGCAATCCTGCTCAATAGCCGAAAGCAAATCAAAGGGGGATTTGGTCGAGGTCTGGAAACGTTGCTGAATGCGTGAACGAATTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACTTTATGGGATAAAGGCAATGATAATGAAATTGGACGGGAgcg                                                                                                                                                     >  1:338187/1‑251 (MQ=255)                                            tAACGGCTCCGCATAAATTTGCCGAACAGAGCGACTTTCACCGTGATAAAGTTGAATCGCCCCAACGCAATCCTGCTCAATAGCCGAAAGCAAATCAAAGGGGGATTTGGTCGAGGTCTGGAAACGTTGCTGAATGCGTGATCGAATTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACTTTATGGGATAAAGGCAATGATAATGAAATTGGACGGGAgcgcg                                                                                                                                                   >  1:458255/1‑251 (MQ=255)                                                   tCCGCATAAATTTGCCGAACAGAGCGACTTTCACCGTGATAAAGTTGAATCGCCCCAACGCAATCCTGCTCAATAGCCGAAAGCAAATCAAAGGGGGATTTGGTCGAGGTCTGGAAACGTTGCTGAATGCGTGAACGAATTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACTTTATGGGATAAAGGCAATGATAATGAAATTGGACGGGAGCGCGCAGAATc                                                                                                                                            <  1:19484/251‑1 (MQ=255)                                                    ccGCATAAATTTGCCGAACAGAGCGACTTTCACCGTGATAAAGTTGAATCGCCCCAACGCAATCCTGCTCAATAGCCGAAAGCAAATCAAAGGGGGATTTGGTCGAGGTCTGGAAACGTTGCTGAATGCGTGAACGAATTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACTTTATGGGATAAAGGCAATGATAATGAAATTGGACGGGAGCGCGCAGAATCa                                                                                                                                           >  1:888750/1‑251 (MQ=255)                                                     cGCATAAATTTGCCGAACAGAGCGACTTTCACCGTGATAAAGTTGAATCGCCCCAACGCAATCCTGCTCAATAGCCGAAAGCAAATCAAAGGGGGATTTGGTCGAGGTCTGGAAACGTTGCTGAATGCGTGAACGAATTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACt                                                                                                                                                                                              <  1:560329/199‑1 (MQ=255)                                                      gCATAAATTTGCCGAACAGAGCGACTTTCACCGTGATAAAGTTGAATCGCCCCAACGCAATCCTGCTCAATAGCCGAAAGCAAATCAAAGGGGGATTTGGTCGAGGTCTGGAAACGTTGCTGAATGCGTGAACGAATTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTg                                                                                                                                                                                                                      >  1:216068/1‑174 (MQ=255)                                                              ttGCCGAACAGAGCGACTTTCACCGTGATAAAGTTGAATCGCCCCAACGCAATCCTGCTCAATAGCCGAAAGCAAATCAAAGGGGGATTTGGTCGAGGTCTGGAAACGTTGCTGAATGCGTGAACGAATTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAACCCGAATCCTCTCGGTAAACTTTATGGGATAAAGGCAATGATAATGAAATTGGACGGGAGCGCGCAGAATCAAGCCATTTTg                                                                                                                                 >  1:427459/1‑251 (MQ=255)                                                                   gAACAGAGCGACTTTCACCGTGATAAAGTTGAATCGCCCCAACGCAATCCTGCTCAATAGCCGAAAGCAAATCAAAGGGGGATTTGGTCGAGGTCTGGAAACGTTGCTGAATGCGTGAACGAATTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACTTTATGGGATAAAGGCAATGATAATGAAATTGGACggg                                                                                                                                                         <  1:750231/222‑1 (MQ=255)                                                                   gAACAGAGCGACTTTCACCGTGATAAAGTTGAATCGCCCCAACGCAATCCTGCTCAATAGCCGAAAGCAAATCAAAGGGGGATTTGGTCGAGGTCTGGAAACGTTGCTGAATGCGTGAACGAATTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACTTTATGGGATAAAGGCAATGATAATGAAATTGGACGGGAGCGCGCAGAATCAAGCCATTTTGGGGGa                                                                                                                            >  1:393053/1‑251 (MQ=255)                                                                            gACTTTCACCGTGATAAAGTTGAATCGCCCCAACGCAATCCTGCTCAATAGCCGAAAGCAAATCAAAGGGGGATTTGGTCGAGGTCTGGAAACGTTGCTGAATGCGTGAACGAATTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACTTTATGGGATAAAGGc                                                                                                                                                                               <  1:369093/191‑1 (MQ=255)                                                                              cTTTCACCGTGATAAAGTTGAATCGCCCCAACGCAATCCTGCTCAATAGCCGAAAGCAAATCAAAGGGGGATTTGGTCGAGGTCTGGAAACGTTGCTGAATGCGTGAACGAATTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACTTTATGGGATAAAGGCAATGATAATGAAATTGGACGGGAGCGCGCAGAATCAAGCCATTTTGGGGGATATTGAAATTc                                                                                                                 <  1:246550/251‑1 (MQ=255)                                                                                      gTGATAAAGTTGAATCGCCCCAACGCAATCCTGCTCAATAGCCGAAAGCAAATCAAAGGGGGATTTGGTCGAGGTCTGGAAACGTTGCTGAATGCGTGAACGAATTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACTTTATGGGATAAAGGCAATGATAATGAAATTg                                                                                                                                                               >  1:703146/1‑197 (MQ=255)                                                                                           aaaGTTGAATCGCCCCAACGCAATCCTGCTCAATAGCCGAAAGCAAATCAAAGGGGGATTTGGTCGAGGTCTGGAAACGTTGCTGAATGCGTGAACGAATTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACTTTATGGGATAAAGGCAATGATAATGAAATTGGACGGGAGCGCGCAGAATCAAGCCATTTTGGGGGATATTGAAATTCCGTCGGCCATTtg                                                                                                    <  1:862991/251‑1 (MQ=255)                                                                                            aaGTTGAATCGCCCCAACGCAATCCTGCTCAATAGCCGAAAGCAAATCAAAGGGGGATTTGGTCGAGGTCTGGAAACGTTGCTGAATGCGTGAACGAATTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACTTTATGGGATAAAGGCAATGATAATGAAATTGGACGGGAGCGCGCAGAATCAAGCCATTTTGGGGGATATTGAAATTCCGTCGGCCATTtgt                                                                                                   >  1:811383/1‑251 (MQ=255)                                                                                              gTTGAATCGCCCCAACGCAATCCTGCTCAATAGCCGAAAGCAAATCAAAGGGGGATTTGGTCGAGGTCTGGAAACGTTGCTGAATGCGTGAACGAATTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACTTTATGGGATAAAGGCAATGATAATg                                                                                                                                                                     <  1:854426/183‑1 (MQ=255)                                                                                                       ccccAACGCAATCCTGCTCAATAGCCGAAAGCAAATCAAAGGGGGATTTGGTCGAGGTCTGGAAACGTTGCTGAATGCGTGAACGAATTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACTTTATGGGATAAAGGCAATGATAATGAAATTGGACGGGAgcgcgc                                                                                                                                                  >  1:857019/1‑193 (MQ=255)                                                                                                       ccccAACGCAATCCTGCTCAATAGCCGAAAGCAAATCAAAGGGGGATTTGGTCGAGGTCTGGAAACGTTGCTGAATGCGTGAACGAATTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACTTTATGGGATAAAGGCAATGATAATGAAATTGGACGGGAGCGCGCAGAATCAAGc                                                                                                                                        <  1:542080/203‑1 (MQ=255)                                                                                                       ccccAACGCAATCCTGCTCAATAGCCGAAAGCAAATCAAAGGGGGATTTGGTCGAGGTCTGGAAACGTTGCTGAATGCGTGAACGAATTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACTTTATGGGATAAAGGCAATGATAATGAAATTGGACGGGAGCGCGCAGAATCAAGCCATTTTggggg                                                                                                                             >  1:415865/1‑214 (MQ=255)                                                                                                       ccccAACGCAATCCTGCTCAATAGCCGAAAGCAAATCAAAGGGGGATTTGGTCGAGGTCTGGAAACGTTGCTGAATGCGTGAACGAATTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACTTTATGGGATAAAGGCAATGATAATGAAATTGGACGGGAGCGCGCAGAATCAAGCCATTTTGGGGGATATTGAAATTCCGTCGGCCATTtg                                                                                                    >  1:395007/1‑239 (MQ=255)                                                                                                       ccccAACGCAATCCTGCTCAATAGCCGAAAGCAAATCAAAGGGGGATTTGGTCGAGGTCTGGAAACGTTGCTGAATGCGTGAACGAATTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACTTTATGGGATAAAGGCAATGATAATGAAATTGGACGGGAGCGCGCAGAATCAAGCCATTTTGGGGGATATTGAAATTCCGTCGGCCATTTGTGAGCTTTCGcc                                                                                        <  1:576971/251‑1 (MQ=255)                                                                                                            aCGCAATCCTGCTCAATAGCCGAAAGCAAATCAAAGGGGGATTTGGTCGAGGTCTGGAAACGTTGCTGAATGCGTGAACGAATTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCctct                                                                                                                                                                                                       <  1:524829/135‑1 (MQ=255)                                                                                                            aCGCAATCCTGCTCAATAGCCGAAAGCAAATCAAAGGGGGATTTGGTCGAGGTCTGGAAACGTTGCTGAATGCGTGAACGAATTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACTTTATGGGATAAAGGCAATGATAATGAAATTGGACGGGAGCGCGCAGAATCAAGCCATTTTGGGGGATATTGAAATTc                                                                                                                 <  1:883806/221‑1 (MQ=255)                                                                                                            aCGCAATCCTGCTCAATAGCCGAAAGCAAATCAAAGGGGGATTTGGTCGAGGTCTGGAAACGTTGCTGAATGCGTGAACGAATTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACTTTATGGGATAAAGGCAATGATAATGAAATTGGACGGGAGCGCGCAGAATCAAGCCATTTTGGGGGATATTGAAATTc                                                                                                                 <  1:723067/221‑1 (MQ=255)                                                                                                            aCGCAATCCTGCTCAATAGCCGAAAGCAAATCAAAGGGGGATTTGGTCGAGGTCTGGAAACGTTGCTGAATGCGTGAACGAATTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACTTTATGGGATAAAGGCAATGATAATGAAATTGGACGGGAGCGCGCAGAATCAAGCCATTTTGGGGGATATTGAAATTCCGTCGGCCATTTGTGAGCTTTCGCCATTCg                                                                                   >  1:489306/1‑251 (MQ=255)                                                                                                            aCGCAATCCTGCTCAATAGCCGAAAGCAAATCAAAGGGGGATTTGGTCGAGGTCTGGAAACGTTGCTGAATGCGTGAACGAATTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACTTTATGGGATAAAGGCAATGATAATGAAATTGGACGGGAGCGCGCAGAATCAAGCCATTTTGGGGGATATTGAAATTCCGTCGGCCATTTGTGAGCTTTCGCCATTCg                                                                                   >  1:757893/1‑251 (MQ=255)                                                                                                               cAATCCTGCTCAATAGCCGAAAGCAAATCAAAGGGGGATTTGGTCGAGGTCTGGAAACGTTGCTGAATGCGTGAACGAATTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACTTTATGGGATAAAGGCAATGATAATGAAATTGGACGGGAGCGCGCAGAATCAAGCCATTTTGGGGGATATTGAAATTCCGTCGGCCATTTGTGAGCTTTCGCCATTCGcc                                                                                 <  1:583869/250‑1 (MQ=255)                                                                                                                 aTCCTGCTCAATAGCCGAAAGCAAATCAAAGGGGGATTTGGTCGAGGTCTGGAAACGTTGCTGAATGCGTGAACGAATTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACTTTATGGGATAAAGGCAATGATAATGAAATTGGACGGGAGCGCGCAGAATCAAGCCATTTTGGGGGATATTGAAATTCCGTCGGCCATTTGTGAGCTTTCGCCATTCGCCGa                                                                               >  1:136717/1‑250 (MQ=255)                                                                                                                 aTCCTGCTCAATAGCCGAAAGCAAATCAAAGGGGGATTTGGTCGAGGTCTGGAAACGTTGCTGAATGCGTGAACGAATTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACTTTATGGGATAAAGGCAATGATAATGAAATTGGACGGGAGCGCGCAGAATCAAGCCATTTTGGGGGATATTGAAATTCCGTCGGCCATTTGTGAGCTTTCGCCATTCGCCGa                                                                               >  1:214783/1‑250 (MQ=255)                                                                                                                 aTCCTGCTCAATAGCCGAAAGCAAATCAAAGGGGGATTTGGTCGAGGTCTGGAAACGTTGCTGAATGCGTGAACGAATTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACTTTATGGGATAAAGGCAATGATAATGAAATTGGACGGGAGCGCGCAGAATCAAGCCATTTTGGGGGATATTGAAATTCCGTCGGCCATTTGTGAGCTTTCGCCATTCGCCGa                                                                               >  1:613336/1‑250 (MQ=255)                                                                                                                  tCCTGCTCAATAGCCGAAAGCAAATCAAAGGGGGATTTGGTCGAGGTCTGGAAACGTTGCTGAATGCGTGAACGAATTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACTTTATGGGATAAAGGCAATGATAATGAAATTGGACGGGAGCGCGCAGAATCAAGCCATTTTGGGGGATATTGAAATTCCGTCGGCCATTTGTGAGCTTTCGCCATTCGCCGAcc                                                                             <  1:803674/251‑1 (MQ=255)                                                                                                                         cAATAGCCGAAAGCAAATCAAAGGGGGATTTGGTCGAGGTCTGGAAACGTTGCTGAATGCGTGAACGAATTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACTTTATGGGATAAAGGCAATGATAATGAAATTGGACGGGAGCGCGCAGAATCAAGCCATTTTGGGGGATATTGAAATTCCGTCGGCCATTTGTGAGCTTTCGCCATTCGCCGACCAGCAAg                                                                       >  1:855511/1‑250 (MQ=255)                                                                                                                         cAATAGCCGAAAGCAAATCAAAGGGGGATTTGGTCGAGGTCTGGAAACGTTGCTGAATGCGTGAACGAATTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACTTTATGGGATAAAGGCAATGATAATGAAATTGGACGGGAGCGCGCAGAATCAAGCCATTTTGGGGGATATTGAAATTCCGTCGGCCATTTGTGAGCTTTCGCCATTCGCCGACCAGCAAg                                                                       >  1:22226/1‑250 (MQ=255)                                                                                                                         cAATAGCCGAAAGCAAATCAAAGGGGGATTTGGTCGAGGTCTGGAAACGTTGCTGAATGCGTGAACGAATTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACTTTATGGGATAAAGGCAATGATAATGAAATTGGACGGGAGCGCGCAGAATCAAGCCATTTTGGGGGATATTGAAATTCCGTCGGCCATTTGTGAGCTTTCGCCATTCGCCGACCAGCAAGc                                                                      <  1:195281/251‑1 (MQ=255)                                                                                                                              gCCGAAAGCAAATCAAAGGGGGATTTGGTCGAGGTCTGGAAACGTTGCTGAATGCGTGAACGAATTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACTTTATGGGATAAAGGCAATGATAATGaaa                                                                                                                                                                  <  1:306075/154‑1 (MQ=255)                                                                                                                              gCCGAAAGCAAATCAAAGGGGGATTTGGTCGAGGTCTGGAAACGTTGCTGAATGCGTGAACGAATTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACTTTATGGGATAAAGGCAATGATAATGAAATTGGACGGGAGCGCGCAGAATCAAGCCATTTTGGGGGATATTGAAATTCCGTCGGCCATTTGTGAGCTTTCGCCATTCGCCGACCAGCAAGCCGtt                                                                  >  1:874763/1‑250 (MQ=255)                                                                                                                                  aaaGCAAATCAAAGGGGGATTTGGTCGAGGTCTGGAAACGTTGCTGAATGCGTGAACGAATTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACTTTATGGGATAAAGGCAATGATAATGAAATTGGACGGGAGCGCGCAGAATCAAGCCATTTTGGGGGATATTGAAATTCCGTCGGCCATTTGTGAGCTTTCGCCATTCGCCGACCAGCAAGCCGTTCATTg                                                             <  1:1810/251‑1 (MQ=255)                                                                                                                                   aaGCAAATCAAAGGGGGATTTGGTCGAGGTCTGGAAACGTTGCTGAATGCGTGAACGAATTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACTTTATGGGATAAAGGCAATGATAATGAAATTGGACGGGAGCGCGCAGAATCAAGCCATTTTGGGGGATATTGAAATTCCGTCGGCCATTTGTGAGCTTTCGCCATTCGCCGACCAGCAAGCCGTTCATTGc                                                            <  1:853951/251‑1 (MQ=255)                                                                                                                                                    aTTTGGTCGAGGTCTGGAAACGTTGCTGAATGCGTGAACGAATTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACTTTATGGGATAAAGGCAATGATAATGAAATTGGACGGGAGCGCGCAGAATCAAGCCATTTTGGGGGATATTGAAATTCCGTCGGCCATTTGTGAGCTTTCGCCATTCGCCGACCAGCAAGCCGTTCATTGCGACTTTCAAGATAGCGt                                           <  1:656356/251‑1 (MQ=255)                                                                                                                                                          tCGAGGTCTGGAAACGTTGCTGAATGCGTGAACGAATTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACTTTATGGGATAAAGGCAATGATAATGAAATTGGACGGGAGCGCGCAGAATCAAGCCATTTTGGGGGATATTGAAATTCCGTCGGCCATTTGTGAGCTTTCGCCATTCGCCGACCAGCAAGCCGTTCATTGCGACTTTCAAGATAGCGTTATTc                                      <  1:771922/250‑1 (MQ=255)                                                                                                                                                               gTCTGGAAACGTTGCTGAATGCGTGAACGAATTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACTTTATggg                                                                                                                                                                                       <  1:218706/100‑1 (MQ=255)                                                                                                                                                               gTCTGGAAACGTTGCTGAATGCGTGAACGAATTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACTTTATGGGATAAAGGCAATGATAATGAAATTGGAc                                                                                                                                                            >  1:18892/1‑127 (MQ=255)                                                                                                                                                               gTCTGGAAACGTTGCTGAATGCGTGAACGAATTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACTTTATGGGATAAAGGCAATGATAATGAAATTGGACGGGAGCGCGCAGAATCAAGCCATTTTGGGGGATATTGAAATTCCGTCGGCCATTTGTGAGCTTTCGCCATTCGCCGACCAGCAAGCCGTTCATTGCGACTTTCAAGATAGCGTTATTc                                      >  1:755437/1‑245 (MQ=255)                                                                                                                                                               gTCTGGAAACGTTGCTGAATGCGTGAACGAATTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACTTTATGGGATAAAGGCAATGATAATGAAATTGGACGGGAGCGCGCAGAATCAAGCCATTTTGGGGGATATTGAAATTCCGTCGGCCATTTGTGAGCTTTCGCCATTCGCCGACCAGCAAGCCGTTCATTGCGACTTTCAAGATAGCGTTATTCATTAc                                 <  1:648996/250‑1 (MQ=255)                                                                                                                                                               gTCTGGAAACGTTGCTGAATGCGTGAACGAATTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACTTTATGGGATAAAGGCAATGATAATGAAATTGGACGGGAGCGCGCAGAATCAAGCCATTTTGGGGGATATTGAAATTCCGTCGGCCATTTGTGAGCTTTCGCCATTCGCCGACCAGCAAGCCGTTCATTGCGACTTTCAAGATAGCGTTATTCATTAc                                 >  1:135679/1‑250 (MQ=255)                                                                                                                                                               gTCTGGAAACGTTGCTGAATGCGTGAACGAATTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACTTTATGGGATAAAGGCAATGATAATGAAATTGGACGGGAGCGCGCAGAATCAAGCCATTTTGGGGGATATTGAAATTCCGTCGGCCATTTGTGAGCTTTCGCCATTCGCCGACCAGCAAGCCGTTCATTGCGACTTTCAAGATAGCGTTATTCATTAc                                 <  1:804030/250‑1 (MQ=255)                                                                                                                                                               gTCTGGAAACGTTGCTGAATGCGTGAACGAATTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACTTTATGGGATAAAGGCAATGATAATGAAATTGGACGGGAGCGCGCAGAATCAAGCCATTTTGGGGGATATTGAAATTCCGTCGGCCATTTGTGAGCTTTCGCCATTCGCCGACCAGCAAGCCGTTCATTGCGACTTTCAAGATAGCGTTATTCATTAc                                 >  1:410357/1‑250 (MQ=255)                                                                                                                                                                    gAAACGTTGCTGAATGCGTGAACGAATTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACTTTATGGGATAAAGGCAATGATAATGAAATTGGACGGGAGCGCGCAGAATCAAGCCATTTTGGGGGATATTGAAATTCCGTCGGCCATTTGTGAGCTTTCGCCATTCGCCGACCAGCAAGCCGTTCATTGCGACTTTCAAGATAGCGTTATTCATTACCACGc                            <  1:771727/250‑1 (MQ=255)                                                                                                                                                                     aaaCGTTGCTGAATGCGTGAACGAATTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACTTTATGGGATAAAGGCAATGATAATGAAATTGGACGGGAGCGCGCAGAATCAAGCCATTTTGGGGGATATTGAAATTCCGTCGGCCATTTGTGAGCTTTCGCCATTCGCCGACCAGCAAGCCGTTCATTGCGACTTTCAAGATAGCGTTATTCATTACCACGcc                           <  1:31821/250‑1 (MQ=255)                                                                                                                                                                          ttGCTGAATGCGTGAACGAATTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACTTTATGGGATAAAGGCAATGATAATGAAATTGGACGGGAGCGCGCAGAATCAAGCCATTTTGGGGGATATTGAAATTCCGTCGGCCATTTGTGAGCTTTCGCCATTCGCCGACCAGCAAGCCGTTCATTGCGACTTTCAAGATAGCGTTATTCATTACCACGCCtcatcg                     <  1:781245/251‑1 (MQ=255)                                                                                                                                                                                    cGTGAACGAATTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAAATTTATGGGATAAAGGCAATGATAATGAAATTGGACGGGGGCGCGCAGAATCAAGCCATTTTGGGGGATATTGAAATTCCGTCGGCCATTTGTGAGCTTTCGCCATTCGCCGACCAGCAAGCCGTTCATTGCGACTTTCAAGATAGCGTTATTCATTACCACGCCTCATCGTCATCGGaa            <  1:760514/250‑1 (MQ=255)                                                                                                                                                                                          cGAATTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACTTTATGGGATAAAGGCAATGATAATGAAATTGGACGGGAGCGCGCAGAATCAAGCCATTTTGGGGGATATTGAAATTCCGTCGGCCATTTGTGAGCTTTCGCCATTCGCCGACCAGCAAGCCGTTCATTGCGACTTTCAAGATAGCGTTATTCATTACCACGCCTCATCGTCATCGGAAAATGCAg     >  1:321065/1‑251 (MQ=255)                                                                                                                                                                                            aaTTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACTTTATGGGATAAAGGCAATGATAATGAAATTGGACGGGAgcgcgc                                                                                                                                                  >  1:218093/1‑108 (MQ=255)                                                                                                                                                                                            aaTTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACTTTATGGGATAAAGGCAATGATAATGAAATTGGACGGGAGCGCGCAGAATCAAGCCATTTTGGGGGATATTGAAATTc                                                                                                                 <  1:529452/141‑1 (MQ=255)                                                                                                                                                                                             aTTTGTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACTTTATGGGATAAAGGCAATGATAATGAAATTGGACGGGAGCGCGCAGAATCAAGCCATTTTGGGGGATATTGAAATTCCGTCGGCCATTTGTGAGCTTTCGCCATTCGCCGACCAGCAAGCCGTTCATTGCGACTTTCAAGATAGCGTTATTCATTACCACGCCTCATCGTCATCGGAAAATGCAGgtg  <  1:27480/251‑1 (MQ=255)                                                                                                                                                                                                 gTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACTTTATGGGATAAAGGCAATGATAATGAAATTGGACGGGAGCGCGCAGAATCAAGCCATTTTGGGGGATATTGAAATTCCGTCGGCCATTTGTGAGCTTTCGCCATTCGCCGACCAGCAAGc                                                                      <  1:540810/179‑1 (MQ=255)                                                                                                                                                                                                 gTTCATTGTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACTTTATGGGATAAAGGCAATGATAATGAAATTGGACGGGAGCGCGCAGAATCAAGCCATTTTGGGGGATATTGAAATTCCGTCGGCCATTTGTGAGCTTTCGCCATTCGCCGACCAGCAAGCCGTTCATTGc                                                            <  1:900159/189‑1 (MQ=255)                                                                                                                                                                                                        |                                                                                                                                                                                                                                                AAAGTGCGGTAGTTTTTCAACACGTTTTACACTTCTTATCATTTAACGGCTCCGCATAAATTTGCCGAACAGAGCGACTTTCACCGTGATAAAGTTGAATCGCCCCAACGCAATCCTGCTCAATAGCCGAAAGCAAATCAAAGGGGGATTTGGTCGAGGTCTGGAAACGTTGCTGAATGCGTGAACGAATTTGTTCATTTTCGGGCAAAAGATTATCGAAGAAATTGTAAACCGAATCCTCTCGGTAAACTTTATGGGATAAAGGCAATGATAATGAAATTGGACGGGAGCGCGCAGAATCAAGCCATTTTGGGGGATATTGAAATTCCGTCGGCCATTTGTGAGCTTTCGCCATTCGCCGACCAGCAAGCCGTTCATTGCGACTTTCAAGATAGCGTTATTCATTACCACGCCTCATCGTCATCGGAAAATGCAGGTG  >  1/1014904‑1015342

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑