Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	306	A→C	intergenic (–/+2124)	– / ← unknown	–/tRNA‑Ala‑GGC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	306	0	A	C	100.0%	39.3 / NA	19	intergenic (–/+2124)	–/unknown	–/tRNA‑Ala‑GGC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (11/8);  total (11/8)

TTATTATCACCAGAGGTGACGATTGGTCGTTGCAGTGGACTGACCAGGACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACAGTTTGATTTAGATCGGGCGCTGGGAGGCGAAGCTTTTGTGCTCGAAGGCGGAAACAAAGCTTTTTTGCTTAAAAAATTAAAAACGTTCAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCGGCATGTGGCGAGAACCCAAGCCAATTAAGGCGCCTTTACGT  >  1/216‑547                                                                                           |                                                                                                                                                                                                                                                  ttcttatCACCAGAGGTGACGATTGGTCGTTGCAGTGGACTGACCAGGACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATaa                                                                                    <  1:45851/247‑1 (MQ=11) ttcttatCACCAGAGGTGACGATTGGTCGTTGCAGTGGACTGACCAGGACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATaa                                                                                    <  1:656074/247‑1 (MQ=11)   cttatCACCAGAGGTGACGATTGGTCGTTGCAGTGGACTGACCAGGACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGAt                                                                                         >  1:65448/2‑243 (MQ=11)                              ttGCAGTGGACTGACCAGGACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGAttt                                                      >  1:49810/1‑251 (MQ=255)                                gCAGTGGACTGACCAGGACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTag                                                            <  1:634013/243‑1 (MQ=255)                                          gACCAGGACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTagag                                                                                                           <  1:663032/186‑1 (MQ=11)                                          gACCAGGACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTagag                                                          >  1:372580/1‑235 (MQ=11)                                          gACCAGGACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCgg                                          >  1:68208/1‑251 (MQ=255)                                               ggACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAgaga                                                                                                          >  1:23072/1‑182 (MQ=11)                                               ggACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCGGCAtgt                                     >  1:617054/1‑251 (MQ=255)                                                     aTCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCGGCATGTGGCgag                               >  1:862477/1‑251 (MQ=255)                                                     aTCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCGGCATGTGGCgag                               >  1:813220/1‑251 (MQ=255)                                                          gtgtTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCGGCATGTGGCGAGAAcc                           >  1:23451/1‑250 (MQ=255)                                                              tgtCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATaa                                                                                    <  1:679958/189‑1 (MQ=11)                                                                tctcGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCGGCATGTGGCGAGAACCCAAGcc                     <  1:473612/250‑1 (MQ=255)                                                                           cAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACgg                                                                     <  1:565227/191‑1 (MQ=11)                                                                           cAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCGGCATGTGGCGAGAACCCAAGCCAATTAAGGCGcc         <  1:104007/251‑1 (MQ=255)                                                                             aGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGAc                                                  >  1:373634/1‑208 (MQ=11)                                                                                  aGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCGGCATGTGGCGAGAACCCAAGCCAATTAAGGCGCCTTTACGt  >  1:153904/1‑251 (MQ=255)                                                                                           |                                                                                                                                                                                                                                                  TTATTATCACCAGAGGTGACGATTGGTCGTTGCAGTGGACTGACCAGGACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACAGTTTGATTTAGATCGGGCGCTGGGAGGCGAAGCTTTTGTGCTCGAAGGCGGAAACAAAGCTTTTTTGCTTAAAAAATTAAAAACGTTCAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCGGCATGTGGCGAGAACCCAAGCCAATTAAGGCGCCTTTACGT  >  1/216‑547

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑