Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	1	865,250	Δ1,249 bp	intergenic (+48052/+3530)	unknown → / ← unknown	tRNA‑Leu‑CAG/tRNA‑Lys‑TTT

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	1	865250	866498	1249	87 [0]	[0] 87	unknown/unknown	tRNA‑Leu‑CAG/tRNA‑Lys‑TTT

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	1	= 865249	0 (0.000)	87 (0.990)	56/426	0.3	100%	intergenic (+48051/+4779)	unknown/unknown	tRNA‑Leu‑CAG/tRNA‑Lys‑TTT
	?	1	866499 =	0 (0.000)					intergenic (+49301/+3529)	unknown/unknown	tRNA‑Leu‑CAG/tRNA‑Lys‑TTT

TGTGCCTTGCATGGGTGATCAATCGGTGAACGACAAAGATCCGAACGGACATCCAATGCGCCCGCACATCGTCTTTTTCGGTGAAAGTGTGCCCGTGTTGGAACCGGCCATTGATTTGGTTTCGCAAGCAGACATTGTGCTGGTCATCGGTACATCCCTGCAAGTTTACCCGGCAAACGGTTTGGTCAACGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTATTCGCCATAACGTCAACGTAGTAAAAATGAAAGCAGGCGAAGGCGTGCCTAAAGTGGTGGCGGAATTATTGGAGAAGGCAAAAAACGGTTAGAAAAATGACCGCACTTTGATCTGCACCCCAAAAGTTGGACATCACACCCAACCAATTAAGGTGCAGATTTTTATGGGCAAACATTATACGACTCATACTGCTATTGGTGTAGGGGCACGCGGGTAAATATTGCCTCACGTATCTCCATCTTACC  >  1/865002‑865495                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       tgtgCCTTGCATGGGTGATCAATCGGTGAACGACAAAGATCCGAACGGACATCCAATGCGCCCGCACATCGTCTTTTTCGGGGAAAGTGTGCCCGTGTTGGAACCGGCCATTGATTTGGTTTCGCAAGCAGACATTGTGCTGGTCATCGGTACATCCCTGCAAGTTTACCCGGCAAACGGTTTGGTCAACGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatc                                                                                                                                                                                                                                                     <  1:439526‑M1/251‑4 (MQ=255)    gCCTTGCATGGGTGATCAATCGGTGAACGACAAAGATCCGAACGGACATCCAATGCGCCCGCACATCGTCTTTTTCGGTGAAAGTGTGCCCGTGTTGGAACCGGCCATTGATTTGGTTTCGCAAGCAGACATTGTGCTGGTCATCGGTACATCCCTGCAAGTTTACCCGGCAAACGGTTTGGTCAACGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatc                                                                                                                                                                                                                                                     >  1:658570‑M1/1‑245 (MQ=255)              ggTGATCAATCGGTGAACGACAAAGATCCGAACGGACATCCAATGCGCCCGCACATCGTCTTTTTCGGTGAAAGTGTGCCCGTGTTGGAACCGGCCATTGATTTGGTTTCGCAAGCAGACATTGTGCTGGTCATCGGTACATCCCTGCAAGTTTACCCGGCAAACGGTTTGGTCAACGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatc                                                                                                                                                                                                                                                     <  1:260393‑M1/238‑4 (MQ=255)                tGATCAATCGGTGAACGACAAAGATCCGAACGGACATCCAATGCGCCCGCACATCGTCTTTTTCGGTGAAAGTGTGCCCGTGTTGGAACCGGCCATTGATTTGGTTTCGCAAGCAGACATTGTGCTGGTCATCGGTACATCCCTGCAAGTTTACCCGGCAAACGGTTTGGTCAACGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtgg                                                                                                                                                                                                                                       <  1:740254‑M1/250‑18 (MQ=255)                   tCAATCGGTGAACGACAAAGATCCGAACGGACATCCAATGCGCCCGCACATCGTCTTTTTCGGTGAAAGTGTGCCCGTGTTGGAACCGGCCATTGATTTGGTTTCGCAAGCAGACATTGTGCTGGTCATCGGTACATCCCTGCAAGTTTACCCGGCAAACGGTTTGGTCAACGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaa                                                                                                                                                                                                                                   <  1:679527‑M1/251‑22 (MQ=255)                    cAATCGGTGAACGACAAAGATCCGAACGGACATCCAATGCGCCCGCACATCGTCTTTTTCGGTGAAAGTGTGCCCGTGTTGGAACCGGCCATTGATTTGGTTTCGCAAGCAGACATTGTGCTGGTCATCGGTACATCCCTGCAAGTTTACCCGGCAAACGGTTTGGTCAACGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaac                                                                                                                                                                                                                                  <  1:595534‑M1/251‑23 (MQ=255)                                    aaGATCCGAACGGACATCCAATGCGCCCGCACATCGTCTTTTTCGGTGAAAGTGTGCCCGTGTTGGAACCGGCCATTGATTTGGTTTCGCAAGCAGACATTGTGCTGGTCATCGGTTCATCCCTGCAAGTTTACCCGGCAAACGGTTTGGTCAACGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaac                                                                                                                                                                                                                  <  1:674750‑M1/251‑39 (MQ=255)                                    aaGATCCGAACGGACATCCAATGCGCCCGCACATCGTCTTTTTCGGTGAAAGTGTGCCCGTGTTGGAACCGGCCATTGATTTGGTTTCGCAAGCAGACATTGTGCTGGTCATCGGTACATCCCTGCAAGTTTACCCGGCAAACGGTTTGGTCAACGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaac                                                                                                                                                                                                                  <  1:539508‑M1/251‑39 (MQ=255)                                    aaGATCCGAACGGACATCCAATGCGCCCGCACATCGTCTTTTTCGGTGAAAGTGTGCCCGTGTTGGAACCGGCCATTGATTTGGTTTCGCAAGCAGACATTGTGCTGGTCATCGGTACATCCCTGCAAGTTTACCCGGCAAACGGTTTGGTCAACGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaac                                                                                                                                                                                                                  <  1:299881‑M1/251‑39 (MQ=255)                                            aaCGGACATCCAATGCGCCCGCACATCGTCTTTTTCGGTGAAAGTGTGCCCGTGTTGGAACCGGCCATTGATTTGGTTTCGCAAGCAGACATTGTGCTGGTCATCGGTACATCCCTGCAAGTTTACCCGGCAAACGGTTTGGTCAACGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagt                                                                                                                                                                                                          <  1:708052‑M1/251‑47 (MQ=255)                                            aaCGGACATCCAATGCGCCCGCACATCGTCTTTTTCGGTGAAAGTGTGCCCGTGTTGGAACCGGCCATTGATTTGGTTTCGCAAGCAGACATTGTGCTGGTCATCGGTACATCCCTGCAAGTTTACCCGGCAAACGGTTTGGTCAACGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataag                                                                                                                                                                                                           <  1:728335‑M1/250‑46 (MQ=255)                                                gACATCCAATGCGCCCGCACATCGTCTTTTTCGGTGAAAGCGTGCCCGTGTTGGAACCGGCCATTGATTTGGTTTCGCAAGCAGACATTGTGCTGGTCATCGGTACATCCCTGCAAGTTTACCCGGCAAACGGTTTGGTCAACGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaac                                                                                                                                                                                                                  <  1:307922‑M1/239‑39 (MQ=255)                                                    tCCAATGCGCCCGCACATCGTCTTTTTCGGTGAAAGTGTGCCCGTGTTGGAACCGGCCATTGATTTGGTTTCGCAAGCAGACATTGTGCTGGTCATCGGTACATCCCTGCAAGTTTACCCGGCAAACGGTTTGGTCAACGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgact                                                                                                                                                                                                   >  1:548958‑M1/1‑197 (MQ=255)                                                             cccGCACATCGTCTTTTTCGGTGAAAGTGTGCCCGTGTTGGAACCGGCCATTGATTTGGTTTCGCAAGCAGACATTGTGCTGGTCATCGGTACATCCCTGCAAGTTTACCCGGCAAACGGTTTGGTCAACGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatggt                                                                                                                                                                                         <  1:826747‑M1/251‑64 (MQ=255)                                                             cccGCACATCGTCTTTTTCGGTGAAAGTGTGCCCGTGTTGGAACCGGCCATTGATTTGGTTTCGCAAGCAGACATTGTGCTGGTCATCGGTACATCCCTGCAAGTTTACCCGGCAAACGGTTTGGTCAACGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatggt                                                                                                                                                                                         <  1:62068‑M1/251‑64 (MQ=255)                                                             cccGCACATCGTCTTTTTCGGTGAAAGTGTGCCCGTGTTGGAACCGGCCATTGATTTGGTTTCGCAAGCAGACATTGTGCTGGTCATCGGTACATCCCTGCAAGTTTACCCGGCAAACGGTTTGGTCAACGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatgg                                                                                                                                                                                          <  1:529020‑M1/250‑63 (MQ=255)                                                             cccGCACATCGTCTTTTTCGGTGAAAGTGTGCCCGTGTTGGAACCGGCCATTGATTTGGTTTCGCAAGCAGACATTGTGCTGGTCATCGGTACATCCCTGCAAGTTTACCCGGCAAACGGTTTGGTCAACGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatgg                                                                                                                                                                                          <  1:628533‑M1/250‑63 (MQ=255)                                                             cccGCACATCGTCTTTTTCGGTGAAAGTGTGCCCGTGTTGGAACCGGCCATTGATTTGGTTTCGCAAGCAGACATTGTGCTGGTCATCGGTACATCCCTGCAAGTTTACCCGGCAAACGGTTTGGTCAACGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatgg                                                                                                                                                                                          <  1:310596‑M1/250‑63 (MQ=255)                                                             cccGCACATCGTCTTTTTCGGTGAAAGTGTGCCCGTGTTGGAACCGGCCATTGATTTGGTTTCGCAAGCAGACATTGTGCTGGTCATCGGTACATCCCTGCAAGTTTACCCGGCAAACGGTTTGGTCAACGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatgg                                                                                                                                                                                          <  1:299124‑M1/250‑63 (MQ=255)                                                              ccGCACATCGTCTTTTTCGGTGAAAGTGTGCCCGTGTTGGAACCGGCCATTGATTTGGTTTCGCAAGCAGACATTGTGCTGGTCATCGGTACATCCCTGCAAGTTTACCCGGCAAACGGTTTGGTCAACGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatggtt                                                                                                                                                                                        <  1:879481‑M1/251‑65 (MQ=255)                                                                   caTCGTCTTTTTCGGTGAAAGTGTGCCCGTGTTGGAACCGGCCATTGATTTGGTTTCGCAAGCAGACATTGTGTTGGTCATCGGTACATCCCTGCAAGTTTACCCGGCAAACGGTTTGGTCAACGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatggttatagc                                                                                                                                                                                   <  1:337482‑M1/251‑70 (MQ=255)                                                                   caTCGTCTTTTTCGGTGAAAGTGTGCCCGTGTTGGAACCGGCCATTGATTTGGTTTCGCAAGCAGACATTGTGCTGGTCATCGGTACATCCCTGCAAGTTTACCCGGCAAACGGTTTGGTCAACGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatggttatagc                                                                                                                                                                                   <  1:178759‑M1/251‑70 (MQ=255)                                                                   caTCGTCTTTTTCGGTGAAAGTGTGCCCGTGTTGGAACCGGCCATTGATTTGGTTTCGCAAGCAGACATTGTGCTGGTCATCGGTACATCCCTGCAAGTTTACCCGGCAAACGGTTTGGTCAACGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatggttatag                                                                                                                                                                                    >  1:313458‑M1/1‑182 (MQ=255)                                                                    aTCGTCTTTTTCGGTGAAAGTGTGCCCGTGTTGGAACCGGCCATTGATTTGGTTTCGCAAGCAGACATTGTGCTGGTCATCGGTACATCCCTGCAAGTTTACCCGGCAAACGGTTTGGTCAACGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatggttatagca                                                                                                                                                                                  <  1:372263‑M1/251‑71 (MQ=255)                                                                    aTCGTCTTTTTCGGTGAAAGTGTGCCCGTGTTGGAACCGGCCATTGATTTGGTTTCGCAAGCAGACATTGTGCTGGTCATCGGTACATCCCTGCAAGTTTACCCGGCAAACGGTTTGGTCAACGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatggttatagca                                                                                                                                                                                  <  1:346809‑M1/251‑71 (MQ=255)                                                                       gTCTTTTTCGGTGAAAGTGTGCCCGTGTTGGAACCGGCCATTGATTTGGTTTCGCAAGCAGACATTGTGCTGGTCATCGGTACATCCCTGCAAGTTTACCCGGCAAACGGTTTGGTCAACGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactggg                                                                                                                                                                                                                                          <  1:659073‑M1/192‑15 (MQ=255)                                                                        tCTTTTTCGGTGAAAGTGTGCCCGTGTTGGAACCGGCCATTGATTTGGTTTCGCAAGCAGACATTGTGCTGGTCATCGGTACATCCCTGCAAGTTTACCCGGCAAACGGTTTGGTCAACGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatggttatagcatac                                                                                                                                                                               <  1:318047‑M1/250‑74 (MQ=255)                                                                         cTTTTTCGGTGAAAGTGTGCCCGTGTTGGAACCGGCCATTGATTTGGTTTCGCAAGCAGACATTGTGCTGGTCATCGGTACATCCCTGCAAGTTTACCCGGCAAACGGTTTGGTCAACGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatggttatagcatacgt                                                                                                                                                                             <  1:100244‑M1/251‑76 (MQ=255)                                                                                 gTGAAAGTGTGCCCGTGTTGGAACCGGCCATTGATTTGGTTTCGCAAGCAGACATTGTGCTGGTCATCGGTACATCCCTGCAAGTTTACCCGGCAAACGGTTTGGTCAACGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcc                                                                                                                                                                                                                                                    >  1:833878‑M1/1‑168 (MQ=255)                                                                                  tGAAAGTGTGCCCGTGTTGGAACCGGCCATTGATTTGGTTTCGCAAGCAGACATTGTGCTGGTCATCGGTACATCCCTGCAAGTTTACCCGGCAAACGGTTTGGTCAACGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatggttatagcatacgttgtggcat                                                                                                                                                                     <  1:293226‑M1/250‑84 (MQ=255)                                                                                     aaGTGTGCCCGTGTTGGAACCGGCCATTGATTTGGTTTCGCAAGCAGACATTGTGCTGGTCATCGGTACATCCCTGCAAGTTTACCCGGCAAACGGTTTGGTCAACGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatggttatagcatacgttgtggcatatat                                                                                                                                                                 <  1:728738‑M1/251‑88 (MQ=255)                                                                                     aaGTGTGCCCGTGTTGGAACCGGCCATTGATTTGGTTTCGCAAGCAGACATTGTGCTGGTCATCGGTACATCCCTGCAAGTTTACCCGGCAAACGGTTTGGTCAACGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatggttatagcatacgttgtggcatatat                                                                                                                                                                 <  1:543002‑M1/251‑88 (MQ=255)                                                                                        tgtgCCCGTGTTGGAACCGGCCATTGATTTGGTTTCGCAAGCAGACATTGTGCTGGTCATCGGTACATCCCTGCAAGTTTACCCGGCAAACGGTTTGGTCAACGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatggttatagcatacgttgtggcatatatatc                                                                                                                                                              <  1:811332‑M1/251‑91 (MQ=255)                                                                                         gtgCCCGTGTTGGAACCGGCCATTGATTTGGTTTCGCAAGCAGACATTGTGCTGGTCATCGGTACATCCCTGCAAGTTTACCCGGCAAACGGTTTGGTCAACGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaac                                                                                                                                                                                                                                  >  1:299760‑M1/1‑160 (MQ=255)                                                                                            cccGTGTTGGAACCGGCCATTGATTTGGTTTCGCAAGCAGACATTGTGCTGGTCATCGGTACATCCCTGCAAGTTTACCCGGCAAACGGTTTGGTCAACGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatggttatagcatacgttgtggcatatatatcaatc                                                                                                                                                          <  1:821851‑M1/251‑95 (MQ=255)                                                                                            cccGTGTTGGAACCGGCCATTGATTTGGTTTCGCAAGCAGACATTGTGCTGGTCATCGGTACATCCCTGCAAGTTTACCCGGCAAACGGTTTGGTCAACGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatggttatagcatacgttgtggcatatatatcaatc                                                                                                                                                          <  1:208531‑M1/251‑95 (MQ=255)                                                                                               gtgtTGGAACCGGCCATTGATTTGGTTTCGCAAGCAGACATTGTGCTGGTCATCGGTACATCCCTGCAAGTTTACCCGGCAAACGGTTTGGTCAACGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatggttatagcatacgttgtggcatatatatcaatcttg                                                                                                                                                       >  1:173387‑M1/1‑154 (MQ=255)                                                                                               gtgtTGGAACCGGCCATTGATTTGGTTTCGCAAGCAGACATTGTGCTGGTCATCGGTACATCCCTGCAAGTTTACCCGGCAAACGGTTTGGTCAACGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatggttatagcatacgttgtggcatatatatcaatcttg                                                                                                                                                       >  1:45577‑M1/1‑154 (MQ=255)                                                                                               gtgtTGGAACCGGCCATTGATTTGGTTTCGCAAGCAGACATTGTGCTGGTCATCGGTACATCCCTGCAAGTTTACCCGGCAAACGGTTTGGTCAACGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatc                                                                                                                                                                                                                                                     <  1:555039‑M1/157‑4 (MQ=255)                                                                                                tgtTGGAACCGGCCATTGATTTGGTTTCGCAAGCAGACATTGTGCTGGTCATCGGTACATCCCTGCAAGTTTACCCGGCAAACGGTTTGGTCAACGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatggttatagcatacgttgtggcatatatatcaatcttgg                                                                                                                                                      >  1:462066‑M1/1‑153 (MQ=255)                                                                                                tgtTGGAACCGGCCATTGATTTGGTTTCGCAAGCAGACATTGTGCTGGTCATCGGTACATCCCTGCAAGTTTACCCGGCAAACGGTTTGGTCAACGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatggttatagcatacgttgtggcatatatatcaatct                                                                                                                                                         >  1:654667‑M1/1‑153 (MQ=255)                                                                                                tgtTGGAACCGGCCATTGATTTGGTTTCGCAAGCAGACATTGTGCTGGTCATCGGTACATCCCTGCAAGTTTACCCGGCAAACGGTTTGGTCAACGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatggttatagcatacgttgtggcatat                                                                                                                                                                   >  1:906‑M1/1‑153 (MQ=255)                                                                                                  ttGGAACCGGCCATTGATTTGGTTTCGCAAGCAGACATTGTGCTGGTCATCGGTACATCCCTGCAAGTTTACCCGGCAAACGGTTTGGTCAACGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatggttatagcatacgttgtggcatatatatcaatcttggac                                                                                                                                                    <  1:162450‑M1/251‑101 (MQ=255)                                                                                                              aTTGATTTGGTTTCGCAAGCAGACATTGTGCTGGTCATCGGTACATCCCTGCAAGTTTACCCGGCAAACGGTTTGGTCAACGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatggttatagcatacgttgtggcatatatatcaatcttggacttaaaatacagg                                                                                                                                        <  1:631483‑M1/251‑113 (MQ=255)                                                                                                                                   gACATTGTGCTGGTCATCGGTACATCCCTGCAAGTTTACCCGGCAAACGGTTTGGTCAACGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatggttatag                                                                                                                                                                                    >  1:316205‑M1/1‑118 (MQ=255)                                                                                                                                    aCATTGTGCTGGTCATCGGTACATCCCTGCAAGTTTACCCGGCAAACGGTTTGGTCAACGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatggttatagcatacgttgtggcatatatatcaatcttggacttaaaatacaggaggagacatggaaagaactgcg                                                                                                                  >  1:706248‑M1/1‑117 (MQ=255)                                                                                                                                       ttGTGCTGGTCATCGGTACATCCCTGCAAGTTTACCCGGCAAACGGTTTGGTCAACGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatggttatagcatacgttgtggcatatatatcaatcttggacttaaaatacaggaggagacatggaaagaactgcgaaa                                                                                                               >  1:843427‑M1/1‑114 (MQ=255)                                                                                                                                         gtgCTGGTCATCGGTACATCCCTGCAAGTTTACCCGGCAAACGGTTTGGTCAACGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatggttatagcatacgttgtggcatatatatcaatcttggacttaaaatacaggaggagacatggaaagaactgcgaaatt                                                                                                             >  1:267929‑M1/1‑112 (MQ=255)                                                                                                                                         gtgCTGGTCATCGGTACATCCCTGCAAGTTTACCCGGCAAACGGTTTGGTCAACGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatggttatagcatacgttgtggcatatatatcaatcttggacttaaaatacaggaggagacatggaaagaactgcgaaatt                                                                                                             >  1:44576‑M1/1‑112 (MQ=255)                                                                                                                                                   tCGGTACATCCCTGCAAGTTTACCCGGCAAACGGTTTGGTCAACGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactggg                                                                                                                                                                                                                                          <  1:674653‑M1/116‑15 (MQ=255)                                                                                                                                                      gTACATCCCTGCAAGTTTACCCGGCAAACGGTTTGGTCAACGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatggttatagcatacgttgtggcatatatatcaatcttggacttaaaatacaggaggagacatggaaagaactgcgaaattattcagaaacgga                                                                                                >  1:494403‑M1/1‑99 (MQ=255)                                                                                                                                                      gTACATCCCTGCAAGTTTACCCGGCAAACGGTTTGGTCAACGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatggttatagcatacgttgtggcatatatatcaatcttggacttaaaatacaggaggagacatggaaagaactgcgaaattattcagaaacgga                                                                                                >  1:663330‑M1/1‑99 (MQ=255)                                                                                                                                                      gTACATCCCTGCAAGTTTACCCGGCAAACGGTTTGGTCAACGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatggttatagcatacgttgtggcatatatatcaatcttggacttaaaatacaggaggagacatggaaagaactgcgaaattattcagaaacgga                                                                                                >  1:817399‑M1/1‑99 (MQ=255)                                                                                                                                                      gTACATCCCTGCAAGTTTACCCGGCAAACGGTTTGGTCAACGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatggttatagcatacgttgtggcatatatatcaatcttggacttaaaatacaggaggagacatggaaagaactgcgaaattattcaga                                                                                                      >  1:804970‑M1/1‑99 (MQ=255)                                                                                                                                                      gTACATCCCTGCAAGTTTACCCGGCAAACGGTTTGGTCAACGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatggttatagcatacgttgtggcatatatatc                                                                                                                                                              >  1:327265‑M1/1‑99 (MQ=255)                                                                                                                                                      gTACATCCCTGCAAGTTTACCCGGCAAACGGTTTGGTCAACGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatgg                                                                                                                                                                                          >  1:899322‑M1/1‑99 (MQ=255)                                                                                                                                                       catctattattcacctatcccgggaaacggtttggtcaaCGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatggttatagcatacgttgtggcatatatatcaatcttggacttaaaatacaggaggagacatggaaagaactgcgaaattattcagaaacgga                                                                                                >  1:155044‑M1/19‑98 (MQ=255)                                                                                                                                                            cccTGCAAGTTTACCCGGCAAACGGTTTGGTCAACGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatggttatagcatacgttgtggcatatatatcaatcttggacttaaaatacaggaggagacatggaaag                                                                                                                         <  1:5774‑M1/220‑128 (MQ=255)                                                                                                                                                             ccTGCAAGTTTACCCGGCAAACGGTTTGGTCAACGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatggttatagcatacgttgtggcatatatatcaatcttggacttaaaatacaggaggagacatggaaagaactgcgaaatta                                                                                                            <  1:631364‑M1/232‑141 (MQ=255)                                                                                                                                                             ccTGCAAGTTTACCCGGCAAACGGTTTGGTCAACGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatggttatagcatacgttgtggcatatatatcaatcttggacttaaaatacagg                                                                                                                                        <  1:782652‑M1/204‑113 (MQ=255)                                                                                                                                                              cTGCAAGTTTACCCGGCAAACGGTTTGGTCAACGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatggttatagcatacgttgtggcatatatatcaatcttggacttaaaatacaggaggagacatggaaagaactgcgaaattattcaga                                                                                                      <  1:2677‑M1/237‑147 (MQ=255)                                                                                                                                                                 cAAGTTTACCCGGCAAACGGTTTGGTCAACGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatggttatagcatacgttgtggcatatatatcaatcttggacttaaaatacaggaggagacatggaaagaactgcgaaattattcagaaacggacgcaatcaagc                                                                                     <  1:449077‑M1/251‑164 (MQ=255)                                                                                                                                                                   aGTTTACCCGGCAAACGGTTTGGTCAACGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatggttatagcatacgttgtggcatatat                                                                                                                                                                 <  1:565560‑M1/173‑88 (MQ=255)                                                                                                                                                                            ggCAAACGGTTTGGTCAACGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatggttatagcatacgttgtggcatatatatcaatcttggacttaaaa                                                                                                                                              <  1:431674‑M1/183‑107 (MQ=255)                                                                                                                                                                                        ggTCAACGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatggttatagcatacgttgtggcatatatatcaatcttggacttaaaatacaggaggagacatggaaagaactgcgaaattattcagaaacggacgcaatcaagccgtacggttaccggtggagttcg                                                              >  1:180519‑M1/1‑65 (MQ=255)                                                                                                                                                                                             aCGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatggttatagcatacgttgtggcatatatatcaatcttggacttaaaatacaggaggagacatggaaagaactgcgaaattattcagaaacggacgcaatcaagccgtacggttaccggtggagttcgaattt                                                         >  1:660427‑M1/1‑60 (MQ=255)                                                                                                                                                                                                   cTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatggttatagcatacgttgtggcatatatatcaatcttggacttaaaatacaggaggagacatggaaagaactgcgaaattattcagaaacggacgcaatcaagccgtacggttaccggtggagttcgaatttgatgc                                                    <  1:829027‑M1/250‑197 (MQ=255)                                                                                                                                                                                                    tCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatggttatagcatacgttgtggcatatatatcaatcttggacttaaaatacaggaggagacatggaaagaactgcgaaattattcagaaacggacgcaatcaagccgtacggttaccggtggagttcgaatttgatg                                                     >  1:876725‑M1/1‑53 (MQ=255)                                                                                                                                                                                                       cAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatggttatagcatacgttgtggcatatatatcaatcttggacttaaaatacaggaggagacatggaaagaactgcgaaattattcagaaacggacgcaatcaagccgtacggttaccggtggagttcgaatttgatgctgaac                                               <  1:340161‑M1/251‑202 (MQ=255)                                                                                                                                                                                                         aGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatggttatagcatacgttgtggcatatatat                                                                                                                                                               <  1:240987‑M1/137‑90 (MQ=255)                                                                                                                                                                                                             cGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatggttatagcatacgttgtggcatatat                                                                                                                                                                 <  1:166153‑M1/131‑88 (MQ=255)                                                                                                                                                                                                               cTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatggttatagcatacgttgtggcatatatatcaatcttggacttaaaatacaggaggagacatggaaagaactgcgaaattattcagaaacggacgcaatcaagccgtacggttaccggtggagttcgaatttgatgctgaacaagtatac                                       <  1:436600‑M1/251‑210 (MQ=255)                                                                                                                                                                                                                  aaaTTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatggttatagcatacgttgtggcatatatatcaatcttggacttaaaatacaggaggagacatggaaagaactgcgaaattattcagaaacggacgcaatcaagccgtacggttaccggtggagttcgaatttgatgctgaacaagtatacatt                                    <  1:863775‑M1/251‑213 (MQ=255)                                                                                                                                                                                                                   aaTTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatggttatagcatacgttgtggcatatatatcaatcttggacttaaaatacaggaggagacatggaaagaactgcgaaattattcagaaacggacgcaatcaagccgtacggttaccggtggagttcgaatttgatgctgaacaagtatac                                       <  1:796036‑M1/247‑210 (MQ=255)                                                                                                                                                                                                                   aaTTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatggttatagcatacgttgtggcatatatatcaatcttggacttaaaatacaggaggagacatggaaagaactgcgaaattattcagaaacggacgcaatcaagccgtacggttaccggtggagttcgaatttgatgctgaacaagtatac                                       >  1:663794‑M1/1‑38 (MQ=255)                                                                                                                                                                                                                   aaTTTACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatggttatagcatacgttgtggcatatatatcaatcttggacttaaaatacaggaggagacatggaaagaactg                                                                                                                    >  1:419127‑M1/1‑38 (MQ=255)                                                                                                                                                                                                                     tttACCTCATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatggttatagcatacgttgtggcatatatatcaatcttggacttaaaatacaggaggagacatggaaagaactgcgaaattattcagaaacggacgcaatcaagccgtacggttaccggtggagttcgaatttgatgctgaacaagtatacattcgt                                 <  1:144530‑M1/251‑216 (MQ=255)                                                                                                                                                                                                                            cATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatggttatagcatacgttgtggcatatatatcaatcttggacttaaaatacaggaggagacatggaaagaactgcgaaattattcagaaacggacgcaatcaagccgtacggttaccggtggagttcgaatttgatgctgaacaagtatacat                                     <  1:314816‑M1/240‑212 (MQ=255)                                                                                                                                                                                                                            cATCGACCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatggttatagcatacgt                                                                                                                                                                             <  1:478177‑M1/104‑76 (MQ=255)                                                                                                                                                                                                                                 aCCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatggttatagcatacgttgtggcatatatatcaatcttggacttaaaatacaggaggagacatggaaagaactgcga                                                                                                                 <  1:665480‑M1/159‑136 (MQ=255)                                                                                                                                                                                                                                 aCCCAAACCCGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatggttatagcatacgttgtggca                                                                                                                                                                      >  1:650396‑M1/1‑24 (MQ=255)                                                                                                                                                                                                                                        cccGAACACAGGGTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatggttatagcatacgttgtggcatatatatcaatcttggacttaaaatacaggaggagacatggaaagaactgcgaaattattcagaaacggacgcaatcaagccgtacggttaccggtggagttcgaatttgatgctgaacaagtatacattcgtaaagaacaaaatggagata              >  1:488495‑M1/1‑17 (MQ=255)                                                                                                                                                                                                                                                   ggTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatggttatagcatacgttgtggcatatatatcaatcttggacttaaaatacaggaggagacatggaaagaactgcgaaattattcagaaacggacgcaatcaagccgtacggttaccggtggagttcgaatttgatgctgaacaagtatacattcgtaaagaacaaaatggagatattgtactt      <  1:621015‑M1/248‑243 (MQ=255)                                                                                                                                                                                                                                                    gTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatggttatagcatacgttgtggcatatatatcaatcttggacttaaaatacaggaggagacatggaaagaactgcgaaattattcagaaacggacgcaatcaagccgtacggttaccggtggagttcgaatttgatgctgaacaagtatacattcgtaaagaacaaaatggagatattgtactttcat  >  1:887416‑M1/1‑5 (MQ=255)                                                                                                                                                                                                                                                    gTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatggttatagcatacgttgtggcatatatatcaatcttggacttaaaatacaggaggagacatggaaagaactgcgaaattattcagaaacggacgcaatcaagccgtacggtta                                                                           <  1:177351‑M1/178‑174 (MQ=255)                                                                                                                                                                                                                                                    gTTTAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatggttatagcatacg                                                                                                                                                                              >  1:665278‑M1/1‑5 (MQ=255)                                                                                                                                                                                                                                                      ttAatcctgttactgggtggtgaacgtcttttgcgataaacagataagttttgactcttttatggttatagcatacgttgtggcatatatatcaat                                                                                                                                                           >  1:712486‑M1/1‑3 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       TGTGCCTTGCATGGGTGATCAATCGGTGAACGACAAAGATCCGAACGGACATCCAATGCGCCCGCACATCGTCTTTTTCGGTGAAAGTGTGCCCGTGTTGGAACCGGCCATTGATTTGGTTTCGCAAGCAGACATTGTGCTGGTCATCGGTACATCCCTGCAAGTTTACCCGGCAAACGGTTTGGTCAACGAAGCTCCCAAGAACGCTCAAATTTACCTCATCGACCCAAACCCGAACACAGGGTTTATTCGCCATAACGTCAACGTAGTAAAAATGAAAGCAGGCGAAGGCGTGCCTAAAGTGGTGGCGGAATTATTGGAGAAGGCAAAAAACGGTTAGAAAAATGACCGCACTTTGATCTGCACCCCAAAAGTTGGACATCACACCCAACCAATTAAGGTGCAGATTTTTATGGGCAAACATTATACGACTCATACTGCTATTGGTGTAGGGGCACGCGGGTAAATATTGCCTCACGTATCTCCATCTTACC  >  1/865002‑865495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑