BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	519,706	+TG	intergenic (+156289/‑52313)	unknown → / → unknown	tRNA‑SeC(p)‑TCA/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	519,703	1	.	G	97.1%	282.0 / 3.9	102	intergenic (+156286/‑52316)	unknown/unknown	tRNA‑SeC(p)‑TCA/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA
Reads supporting (aligned to +/- strand): ref base . (1/2); new base G (64/35); total (65/37)
Fisher's exact test for biased strand distribution p-value = 2.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01
*	1	519,703	2	.	T	97.1%	288.2 / 2.4	102	intergenic (+156286/‑52316)	unknown/unknown	tRNA‑SeC(p)‑TCA/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA
Reads supporting (aligned to +/- strand): ref base . (1/2); new base T (64/35); total (65/37)
Fisher's exact test for biased strand distribution p-value = 2.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01

Only 100 of 103 total aligned reads displayed.

GGGATAATAACAAAATGGCTAAGGAGTTATTTTCTACTTTGAAAAAGCTTTTAATTAAATAATCGTGTAGATGGAACTTATCCTCCCTTGCTGACGCGCGCCGGTATGAATAAAAACGTTTAAAAAAATGACCGCACTTTTATTCGATATGCAAATGGTAAATCAGAGAAAATAACATGAAAACATTAGGTATTTTAGGTGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCG‑‑GTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATTGTGCAATGCCAAAAATCAGGAAATTGGGCTAAAGCAGGTGAAATTTTGGCAGAGCAGGCGCGTTTGCTGGAAAGTATTGGGGCGGAAGGTATTTTAATTGGTGCCAATACCATGCACAAAGTCGCGGATGCCGTCCAAAATGTCATTTCTGTGCCGTTGTTACATA  >  1/519453‑519938

||

gggATAATAACAAAATGGCTAAGGAGTTATTTTCTACTTTGAAAAAGCTTTTAATTAAATAATCGTGTAGATGGAACTTATCCTCCCTTGCTGACGCGCGCCGGTATGAATAAAAACGTTTAAAAAAATGACCGCACTTTTATTCGATATGCAAATGGTAAATCAGAGAAAATAACATGAAAACATTAGGTATTTTAGGTGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCg                                                                                                                                                                                                                                               >  1:558360/1‑251 (MQ=255)

       taaCAAAATGGCTAAGGAGTTATTTTCTACTTTGAAAAAGCTTTTAATTAAATAATCGTGTAGATGGAACTTATCCTCCCTTGCTGACGCGCGCCGGTATGAATAAAAACGTTTAAAAAAATGACCGCACTTTTATTCGATATGCAAATGGTAAATCAGAGAAAATAACATGAAAACATTAGGTATTTTAGGTGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCG‑‑GTgtgaa                                                                                                                                                                                                                                      >  1:769274/1‑247 (MQ=255)

         aCAAAATGGCTAAGGAGTTATTTTCTACTTTGAAAAAGCTTTTAATTAAATAATCGTGTAGATGGAACTTATCCTCCCTTGCTGACGCGCGCCGGTATGAATAAAAACGTTTAAAAAAATGACCGCACTTTTATTCGATATGCAAATGGTAAATCAGAGAAAATAACATGAAAACATTAGGTATTTTAGGTGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCG‑‑GTgtgaatc                                                                                                                                                                                                                                    <  1:704401/251‑7 (MQ=255)

            aaaTGGCTAAGGAGTTATTTTCTACTTTGAAAAAGCTTTTAATTAAATAATCGTGTAGATGGAACTTATCCTCCCTTGCTGACGCGCGCCGGTATGAATAAAAACGTTTAAAAAAATGACCGCACTTTTATTCGATATGCAAATGGTAAATCAGAGAAAATAACATGAAAACATTAGGTATTTTAGGTGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCG‑‑GTgtgaatcagg                                                                                                                                                                                                                                 <  1:108127/251‑10 (MQ=255)

             aaTGGCTAAGGAGTTATTTTCTACTTTGAAAAAGCTTTTAATTAAATAATCGTGTAGATGGAACTTATCCTCCCTTGCTGACGCGCGCCGGTATGAATAAAAACGTTTAAAAAAATGACCGCACTTTTATTCGATATGCAAATGGTAAATCAGAGAAAATAACATGAAAACATTAGGTATTTTAGGTGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGc                                                                                                                                                                                                                                  <  1:557322/251‑1 (MQ=255)

               tGGCTAAGGAGTTATTTTCTACTTTGAAAAAGCTTTTAATTAAATAATCGTGTAGATGGAACTTATCCTCCCTTGCTGACGCGCGCCGGTATGAATAAAAACGTTTAAAAAAATGACCGCACTTTTATTCGATATGCAAATGGTAAATCAGAGAAAATAACATGAAAACATTAGGTATTTTAGGTGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGt                                                                                                                                                                                                                                <  1:112476/251‑1 (MQ=255)

                ggCTAAGGAGTTATTTTCTACTTTGAAAAAGCTTTTAATTAAATAATCGTGTAGATGGAACTTATCCTCCCTTGCTGACGCGCGCCGGTATGAATAAAAACGTTTAAAAAAATGACCGCACTTTTATTCGATATGCAAATGGTAAATCAGAGAAAATAACATGAAAACATTAGGTATTTTAGGTGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGtt                                                                                                                                                                                                                               >  1:620357/1‑251 (MQ=255)

                 gCTAAGGAGTTATTTTCTACTTTGAAAAAGCTTTTAATTAAATAATCGTGTAGATGGAACTTATCCTCCCTTGCTGACGCGCGCCGGTATGAATAAAAACGTTTAAAAAAATGACCGCACTTTTATTCGATATGCAAATGGTAAATCAGAGAAAATAACATGAAAACATTAGGTATTTTAGGTGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTg                                                                                                                                                                                                                              >  1:59926/1‑251 (MQ=255)

                       gAGTTATTTTCTACTTTGAAAAAGCTTTTAATTAAATAATCGTGTAGATGGAACTTATCCTCCCTTGCTGACGCGCGCCGGTATGAATAAAAACGTTTAAAAAAATGACCGCACTTTTATTCGATATGCAAATGGTAAATCAGAGAAAATAACATGAAAACATTAGGTATTTTAGGTGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTgg                                                                                                                                                                                                                         >  1:560533/1‑250 (MQ=255)

                       gAGTTATTTTCTACTTTGAAAAAGCTTTTAATTAAATAATCGTGTAGATGGAACTTATCCTCCCTTGCTGACGCGCGCCGGTATGAATAAAAACGTTTAAAAAAATGACCGCACTTTTATTCGATATGCAAATGGTAAATCAGAGAAAATAACATGAAAACATTAGGTATTTTAGGTGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTgg                                                                                                                                                                                                                         >  1:819256/1‑250 (MQ=255)

                         gTTATTTTCTACTTTGAAAAAGCTTTTAATTAAATAATCGTGTAGATGGAACTTATCCTCCCTTGCTGACGCGCGCCGGTATGAATAAAAACGTTTAAAAAAATGACCGCACTTTTATTCGATATGCAAATGGTAAATCAGAGAAAATAACATGAAAACATTAGGTATTTTAGGTGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCaa                                                                                                                                                                                                                      >  1:456431/1‑251 (MQ=255)

                                            aaGCTTTTAATTAAATAATCGTGTAGATGGAACTTATCCTCCCTTGCTGACGCGCGCCGGTATGAATAAAAACGTTTAAAAAAATGACCGCACTTTTATTCGATATGCAAATGGTAAATCAGAGAAAATAACATGAAAACATTAGGTATTTTAGGTGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTg                                                                                                                                                                                                   <  1:765511/251‑1 (MQ=255)

                                               cTTTTAATTAAATAATCGTGTAGATGGAACTTATCCTCCCTTGCTGACGCGCGCCGGTATGAATAAAAACGTTTAAAAAAATGACCGCACTTTTATTCGATATGCAAATGGTAAATCAGAGAAAATAACATGAAAACATTAGGTATTTTAGGTGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGa                                                                                                                                                                                                  <  1:792364/249‑1 (MQ=255)

                                                      ttaaATAATCGTGTAGATGGAACTTATCCTCCCTTGCTGACGCGCGCCGGTATGAATAAAAACGTTTAAAAAAATGACCGCACTTTTATTCGATATGCAAATGGTAAATCAGAGAAAATAACATGAAAACATTAGGTATTTTAGGTGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCg                                                                                                                                                                                         >  1:705178/1‑251 (MQ=255)

                                                        aaaTAATCGTGTAGATGGAACTTATCCTCCCTTGCTGACGCGCGCCGGTATGAATAAAAACGTTTAAAAAAATGACCGCACTTTTATTCGATATGCAAATGGTAAATCAGAGAAAATAACATGAAAACATTAGGTATTTTAGGTGGGATGAGTCCCGATAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGc                                                                                                                                                                                                                        <  1:733393/218‑1 (MQ=255)

                                                        aaaTAATCGTGTAGATGGAACTTATCCTCCCTTGCTGACGCGCGCCGGTATGAATAAAAACGTTTAAAAAAATGACCGCACTTTTATTCGATATGCAAATGGTAAATCAGAGAAAATAACATGAAAACATTAGGTATTTTAGGTGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGt                                                                                                                                                                                               <  1:513896/243‑1 (MQ=255)

                                                        aaaTAATCGTGTAGATGGAACTTATCCTCCCTTGCTGACGCGCGCCGGTATGAATAAAAACGTTTAAAAAAATGACCGCACTTTTATTCGATATGCAAATGGTAAATCAGAGAAAATAACATGAAAACATTAGGTATTTTAGGTGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGt                                                                                                                                                                                        >  1:663600/1‑250 (MQ=255)

                                                          ataatCGTGTAGATGGAACTTATCCTCCCTTGCTGACGCGCGCCGGTATGAATAAAAACGTTTAAAAAAATGACCGCACTTTTATTCGATATGCAAATGGTAAATCAGAGAAAATAACATGAAAACATTAGGTATTTTAGGTGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCaa                                                                                                                                                                                     >  1:740106/1‑251 (MQ=255)

                                                                gtgtAGATGGAACTTATCCTCCCTTGCTGACGCGCGCCGGTATGAATAAAAACGTTTAAAAAAATGACCGCACTTTTATTCGATATGCAAATGGTAAATCAGAGAAAATAACATGAAAACATTAGGTATTTTAGGTGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGt                                                                                                                                                                                               >  1:781637/1‑235 (MQ=255)

                                                                     gATGGAACTTATCCTCCCTTGCTGACGCGCGCCGGTATGAATAAAAACGTTTAAAAAAATGACCGCACTTTTATTCGATATGCAAATGGTAAATCAGAGAAAATAACATGAAAACATTAGGTATTTTAGGTGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTg                                                                                                                                                                                                   <  1:426887/226‑1 (MQ=255)

                                                                     gATGGAACTTATCCTCCCTTGCTGACGCGCGCCGGTATGAATAAAAACGTTTAAAAAAATGACCGCACTTTTATTCGATATGCAAATGGTAAATCAGAGAAAATAACATGAAAACATTAGGTATTTTAGGTGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAgaga                                                                                                                                                                          >  1:261497/1‑251 (MQ=255)

                                                                     gATGGAACTTATCCTCCCTTGCTGACGCGCGCCGGTATGAATAAAAACGTTTAAAAAAATGACCGCACTTTTATTCGATATGCAAATGGTAAATCAGAGAAAATAACATGAAAACATTAGGTATTTTAGGTGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAgaga                                                                                                                                                                          >  1:285147/1‑251 (MQ=255)

                                                                     gATGGAACTTATCCTCCCTTGCTGACGCGCGCCGGTATGAATAAAAACGTTTAAAAAAATGACCGCACTTTTATTCGATATGCAAATGGTAAATCAGAGAAAATAACATGAAAACATTAGGTATTTTAGGTGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAgaga                                                                                                                                                                          >  1:851342/1‑251 (MQ=255)

                                                                         gAACTTATCCTCCCTTGCTGACGCGCGCCGGTATGAATAAAAACGTTTAAAAAAATGACCGCACTTTTATTCGATATGCAAATGGTAAATCAGAGAAAATAACATGAAAACATTAGGTATTTTAGGTGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATtgt                                                                                                                                                                      >  1:855579/1‑251 (MQ=255)

                                                                         gAACTTATCCTCCCTTGCTGACGCGCGCCGGTATGAATAAAAACGTTTAAAAAAATGACCGCACTTTTATTCGATATGCAAATGGTAAATCAGAGAAAATAACATGAAAACATTAGGTATTTTAGGTGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATtgt                                                                                                                                                                      >  1:224510/1‑251 (MQ=255)

                                                                          aaCTTATCCTCCCTTGCTGACGCGCGCCGGTATGAATAAAAACGTTTAAAAAAATGACCGCACTTTTATTCGATATGCAAATGGTAAATCAGAGAAAATAACATGAAAACATTAGGTATTTTAGGTGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATtgtg                                                                                                                                                                     >  1:662345/1‑251 (MQ=255)

                                                                          aaCTTATCCTCCCTTGCTGACGCGCGCCGGTATGAATAAAAACGTTTAAAAAAATGACCGCACTTTTATTCGATATGCAAATGGTAAATCAGAGAAAATAACATGAAAACATTAGGTATTTTAGGTGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATtgtg                                                                                                                                                                     >  1:364715/1‑251 (MQ=255)

                                                                            cTTATCCTCCCTTGCTGACGCGCGCCGGTATGAATAAAAACGTTTAAAAAAATGACCGCACTTTTATTCGATATGCAAATGGTAAATCAGAGAAAATAACATGAAAACATTAGGTATTTTAGGTGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATTGTGCa                                                                                                                                                                   <  1:317910/251‑1 (MQ=255)

                                                                                tcctccCTTGCTGACGCGCGCCGGTATGAATAAAAACGTTTAAAAAAATGACCGCACTTTTATTCGATATGCAAATGGTAAATCAGAGAAAATAACATGAAAACATTAGGTATTTTAGGTGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATTGTGCAATGc                                                                                                                                                               <  1:882263/251‑1 (MQ=255)

                                                                                    cccTTGCTGACGCGCGCCGGTATGAATAAAAACGTTTAAAAAAATGACCGCACTTTTATTCGATATGCAAATGGTAAATCAGAGAAAATAACATGAAAACATTAGGTATTTTAGGTGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGt                                                                                                                                                                                        >  1:318585/1‑222 (MQ=255)

                                                                                    cccTTGCTGACGCGCGCCGGTATGAATAAAAACGTTTAAAAAAATGACCGCACTTTTATTCGATATGCAAATGGTAAATCAGAGAAAATAACATGAAAACATTAGGTATTTTAGGTGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATTGTGCAATGCCaaa                                                                                                                                                           >  1:463128/1‑251 (MQ=255)

                                                                                    cccTTGCTGACGCGCGCCGGTATGAATAAAAACGTTTAAAAAAATGACCGCACTTTTATTCGATATGCAAATGGTAAATCAGAGAAAATAACATGAAAACATTAGGTATTTTAGGTGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATTGTGCAATGCCaaa                                                                                                                                                           >  1:329048/1‑251 (MQ=255)

                                                                                                          tGAATAAAAACGTTTAAAAAAATGACCGCACTTTTATTCGATATGCAAATGGTAAATCAGAGAAAATAACATGAAAACATTAGGTATTTTAGGTGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATTGTGCAATGCCAAAAATCAGGAAATTGGGCTAAAGc                                                                                                                                     <  1:844084/251‑1 (MQ=255)

                                                                                                           gAATAAAAACGTTTAAAAAAATGACCGCACTTTTATTCGATATGCAAATGGTAAATCAGAGAAAATAACATGAAAACATTAGGTATTTTAGGTGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTg                                                                                                                                                                                                   <  1:862293/188‑1 (MQ=255)

                                                                                                           gAATAAAAACGTTTAAAAAAATGACCGCACTTTTATTCGATATGCAAATGGTAAATCAGAGAAAATAACATGAAAACATTAGGTATTTTAGGTGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATTGTGCAATGCCAAAAATCAGGAAATTGGGCTAAAGCa                                                                                                                                    >  1:833804/1‑251 (MQ=255)

                                                                                                           gAATAAAAACGTTT‑AAAAAATGACCGCACTTTTATTCGATATGCAAATGGTAAATCAGAGAAAATAACATGAAAACATTAGGTATTTTAGGTGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCg                                                                                                                                                                                             >  1:382167/1‑193 (MQ=255)

                                                                                                               aaaaaCGTTTAAAAAAATGACCGCACTTTTATTCGATATGCAAATGGTAAATCAGAGAAAATAACATGAAAACATTAGGTATTTTAGGTGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATTGTGCAATGCCAAAAATCAGGAAATTGGGCTAAAGCAGGTg                                                                                                                                <  1:692303/251‑1 (MQ=255)

                                                                                                                                        cTTTTATTCGATATGCAAATGGTAAATCAGAGAAAATAACATGAAAACATTAGGTATTTTAGGTGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATTGTGCAATGCCAAAAATCAGGAAATTGGGCTAAAGCAGGTGAAATTTTGGCAGAGCAGGCGCGttt                                                                                                       >  1:19619/1‑251 (MQ=255)

                                                                                                                                               tCGATATGCAAATGGTAAATCAGAGAAAATAACATGAAAACATTAGGTATTTTAGGTGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATTGTGCAATGCCAAAAATCAGGAAATTGGGCTAAAGCAGGTGAAATTTTGGCAGAGCAGGCGCGTTTGCTGGaa                                                                                                <  1:829598/251‑1 (MQ=255)

                                                                                                                                                  atatGCAAATGGTAAATCAGAGAAAATAACATGAAAACATTAGGTATTTTAGGTGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATTGTGCAATGCCAAAAATCAGGAAATTGGGCTAAAGCAGGTGAAATTTTGGCAGAGCAGGCGCGTTTGCTGGAAAGt                                                                                             >  1:480304/1‑251 (MQ=255)

                                                                                                                                                     tGCAAATGGTAAATCAGAGAAAATAACATGAAAACATTAGGTATTTTAGCTGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGCGCGTCAATTTTGAAGAGATTGTGCAATGCCAAAAATCAGGAAATTGGGCTAAAGCAGGTGAAATTTTGGCAGAGCAGGCGCGTTTGCTGGAAAGTAtt                                                                                          >  1:224891/1‑251 (MQ=255)

                                                                                                                                                      gCAAATGGTAAATCAGAGAAAATAACATGAAAACATTAGGTATTTTAGGTGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATTGTGCAATGCCAAAAATCAGGAAATTGGGCTAAAGCAGGTGAAATTTTGGCAGAGCAGGCGCGTTTGCTGGAAAGTATTg                                                                                         >  1:762207/1‑251 (MQ=255)

                                                                                                                                                         aaTGGTAAATCAGAGAAAATAACATGAAAACATTAGGTATTTTAGGTGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATTGTGCAATGCCAAAAATCAGGAAATTGGGCTAAAGCAGGTGAAATTTTGGCAGAGCAGGCGCGTTTGCTGGAAAGTATTgggg                                                                                      <  1:319917/251‑1 (MQ=255)

                                                                                                                                                         aaTGGTAAATCAGAGAAAATAACATGAAAACATTAGGTATTTTAGGTGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATTGTGCAATGCCAAAAATCAGGAAATTGGGCTAAAGCAGGTGAAATTTTGGCAGAGCAGGCGCGTTTGCTGGAAAGTATTgggg                                                                                      <  1:835673/251‑1 (MQ=255)

                                                                                                                                                           tGGTAAATCAGAGAAAATAACATGAAAACATTAGGTATTTTAGGTGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATTGTGCAATGCCAAAAATCAGGAAATTGGGCTAAAGCAGGTGAAATTTTGGCAGAGCAGGCGCGTTTGCTGGAAAGTATTGGGGCg                                                                                    >  1:550334/1‑251 (MQ=255)

                                                                                                                                                           tGGTAAATCAGAGAAAATAACATGAAAACATTAGGTATTTTAGGTGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATTGTGCAATGCAAAAAATCAGGAAATTGGGCTAAAGCAGGTGAAATTTTGGCAGAGCAGGCGCGTTTGCTGGAAAGTATTGGGGCg                                                                                    >  1:544207/1‑251 (MQ=255)

                                                                                                                                                                          aaTAACATGAAAACATTAGGTATTTTAGGTGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATTGTGCAATGCCAAAAATCAGGAAATTGGGCTAAAGCAGGTGAAATTTTGGCAGAGCAGGCGCGTTTGCTGGAAAGTATTGGGGCGGAAGGTATTTTAAtt                                                                     >  1:647547/1‑251 (MQ=255)

                                                                                                                                                                              aCATGAAAACATTAGGTATTTTAGGTGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATTGTGCAATGCCAAAAATCAGGAAATTGGGCTAAAGCAGGTGAAATTTTGGCAGAGCAGGCGCGTTTGCTGGAAAGTATTGGGGCGGAAGGTATTTTAATTGGTg                                                                 >  1:593127/1‑251 (MQ=255)

                                                                                                                                                                               cATGAAAACATTAGGTAATTTAGGTGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATTGTGCAATGCCAAAAATCAGGAAATTGGGCTAAAGCAGGTGAAATTTTGGCAGAGCAGGCGCGTTTGCTGGAAAGTATTGGGGCGGAAGGTATTTTAATTGGTg                                                                 >  1:821537/1‑250 (MQ=255)

                                                                                                                                                                                     aaCATTAGGTATTTTAGGTGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATTGTGCAATGCCAAAAATCAGGAAATTGGGCTAAAGCAGGTGAAATTTTGGCAGAGCAGGCGCGTTTGCTGGAAAGTATTGGGGCGGAAGGTATTTTAATTGGTGCCAATAc                                                          <  1:878220/251‑1 (MQ=255)

                                                                                                                                                                                     aaCATTAGGTATTTTAGGTGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATTGTGCAATGCCAAAAATCAGGAAATTGGGCTAAAGCAGGTGAAATTTTGGCAGAGCAGGCGCGTTTGCTGGAAAGTATTGGGGCGGAAGGTATTTTAATTGGTGCCAATAc                                                          <  1:141503/251‑1 (MQ=255)

                                                                                                                                                                                     aaCATTAGGTATTTTAGGTGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATTGTGCAATGCCAAAAATCAGGAAATTGGGCTAAAGCAGGTGAAATTTTGGCAGAGCAGGCGCGTTTGCTGGAAAGTATTGGGGCGGAAGGTATTTTAATTGGTGCCAATAc                                                          <  1:376258/251‑1 (MQ=255)

                                                                                                                                                                                      aCATTAGGTATTTTAGGTGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATTGTGCAATGCCAAAAATCAGGAAATTGGGCTAAAGCAGGTg                                                                                                                                <  1:872032/180‑1 (MQ=255)

                                                                                                                                                                                       cATTAGGTATTTTAGGTGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATTGTGCAATGCCAAAAATCAGGAAATTGGGCTAAAGCAGGTGAAATTTTGGCAGAGCAGGCGCGTTTGCTGGAAAGTATTGGGGCGGAAGGTATTTTAATTGGTGCCAATACCa                                                        <  1:224189/251‑1 (MQ=255)

                                                                                                                                                                                             gTATTTTAGGTGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGc                                                                                                                                                                                                                                  >  1:372294/1‑75 (MQ=255)

                                                                                                                                                                                             gTATTTTAGGTGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAag                                                                                                                                                                             >  1:307886/1‑128 (MQ=255)

                                                                                                                                                                                             gTATTTTAGGTGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATTGTGCAATGCCAAAAATCAGGAAATTGGGCTAAAGCAGGTGAAATTTTGGCAGAGCAGGCGCGTTTGCTGGAAAGTATTGGGGCGGAAGGTATTTTAAt                                                                      <  1:343708/231‑1 (MQ=255)

                                                                                                                                                                                             gTATTTTAGGTGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATTGTGCAATGCCAAAAATCAGGAAATTGGGCTAAAGCAGGTGAAATTTTGGCAGAGCAGGCGCGTTTGCTGGAAAGTATTGGGGCGGAAGGTATTTTAAt                                                                      <  1:748060/231‑1 (MQ=255)

                                                                                                                                                                                             gTATTTTAGGTGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATTGTGCAATGCCAAAAATCAGGAAATTGGGCTAAAGCAGGTGAAATTTTGGCAGAGCAGGCGCGTTTGCTGGAAAGTATTGGGGCGGAAGGTATTTTAATTGGTGCCAATACCATGcaca                                                  >  1:107482/1‑251 (MQ=255)

                                                                                                                                                                                             gTATTTTAGGTGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATTGTGCAATGCCAAAAATCAGGAAATTGGGCTAAAGCAGGTGAAATTTTGGCAGAGCAGGCGCGTTTGCTGGAAAGTATTGGGGCGGAAGGTATTTTAATTGGTGCCAATACCATGcaca                                                  >  1:753235/1‑251 (MQ=255)

                                                                                                                                                                                               aTTTTAGGTGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATTGTGCAATGCCAAAAATCAGGAAATTGGGCTAAAGCAGGTGAAATTTTGGCAGAGCAGGCGCGTTTGCTGGAAAGTATTgggg                                                                                      >  1:493758/1‑213 (MQ=255)

                                                                                                                                                                                               aTTTTAGGTGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATTGTGCAATGCCAAAAATCAGGAAATTGGGCTAAAGCAGGTGAAATTTTGGCAGAGCAGGCGCGTTTGCTGGAAAGTATTGGGGCGGAAGGTATTTTAATTGGTGCCAATACCATGCACaaa                                                >  1:150122/1‑251 (MQ=255)

                                                                                                                                                                                               aTTTTAGGTGGGATGAGTCCCGAAAGCACGGTTTCCTAGTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATTGTGCAATGCCAAAAATCAGGAAATTGGGCTAAAGCAGGTGAAATTTTGGCAGAGCAGGCGCGTTTGCTGGAAAGTAt                                                                                           <  1:444247/208‑1 (MQ=255)

                                                                                                                                                                                                ttttAGGTGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATTGTGc                                                                                                                                                                    >  1:566150/1‑134 (MQ=255)

                                                                                                                                                                                                ttttAGGTGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATTGTGCAATGCCAAAAATCAGGAAATTGGGCTAAAGCAGGTGAAATTTTGGCAGAGCAGGCGCGTTTGCTGGAAAGTATTGGGGCGGAAGGTATTTTAATTGGTGCCAATACCATGCACAAAg                                               >  1:154890/1‑251 (MQ=255)

                                                                                                                                                                                                ttttAGGTGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATTGTGCAATGCCAAAAATCAGGAAATTGGGCTAAAGCAGGTAAAATTTTGGCAGAGCAGGCGCGTTTGCTGGAAAGTATTGGGGCGGAAGGTATTTTAATTGGTGCCAATACCATGCACAAAg                                               >  1:447607/1‑251 (MQ=255)

                                                                                                                                                                                                       tGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATTGTGCAATGCCAAAAATCAGGAAATTGGGCTAAAGCAGGTGAAATTTTGGCAGAGCAGGCGCGTTTGCTGGAAAGTATTgggg                                                                                      >  1:274560/1‑205 (MQ=255)

                                                                                                                                                                                                         ggATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATTGTGCAATGCCAAAAATCAGGAAATTGGGCTAAAGCAGGTGAAATTTTGGCAGAGCAGGCGCGTTTGCTGGAAAGTATTGGGGCGGAAGGTATTTTAATTGGTGCCAATACCATGCACAAAGTCGCGGAt                                       <  1:481849/250‑1 (MQ=255)

                                                                                                                                                                                                           aTGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATTGTGCAATGCCAAAAATCAGGAAATTGGGCTAAAGCAGGTGAAATTTTGGCAGAGCAGGCGCGTTTGCTGGAAAGTATTGGGGCGGAAGGTATTTTAATTGGTGCCAATACCATGCACAAAGTCGCGGATGc                                     <  1:544641/250‑1 (MQ=255)

                                                                                                                                                                                                           aTGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATTGTGCAATGCCAAAAATCAGGAAATTGGGCTAAAGCAGGTGAAATTTTGGCAGAGCAGGCGCGTTTGCTGGAAAGTATTGGGGCGGAAGGTATTTTAATTGGTGCCAATACCATGCACAAAGTCGCGGATGc                                     <  1:696295/250‑1 (MQ=255)

                                                                                                                                                                                                                 cccGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATTGTGCAATGCCAAAAATCAGGAAATTGGGCTAAAGCAGGTGAAATTTTGGCAGAGCAGGCGCGTTTGCTGGAAAGTATTGGGGCGGAAGGTATTTTAATTGGTGCCAATACCATGCACAAAGTCGCGGATGCCGTCCaa                              >  1:319098/1‑251 (MQ=255)

                                                                                                                                                                                                                 cccGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATTGTGCAATGCCAAAAATCAGGAAATTGGGCTAAAGCAGGTGAAATTTTGGCAGAGCAGGCGCGTTTGCTGGAAAGTATTGGGGCGGAAGGTATTTTAATTGGTGCCAATACCATGCACAAAGTCGCGGATGCCGTCCaa                              >  1:697319/1‑251 (MQ=255)

                                                                                                                                                                                                                     aaaGCACGGTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATTGTGCAATGCCAAAAATCAGGAAATTGGGCTAAAGCAGGTGAAATTTTGGCAGAGCAGGCGCGTTTGCTGGAAAGTATTGGGGCGGAAGGTATTTTAATTGGTGCCAATACCATGCACAAAGTCGCGGATGCCGTCCAAAATg                          <  1:788924/251‑1 (MQ=255)

                                                                                                                                                                                                                             gTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATTGTGCAATGCCAAAAATCAGGAAATTGGGCTAAAGc                                                                                                                                     >  1:73136/1‑136 (MQ=255)

                                                                                                                                                                                                                             gTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATTGTGCAATGCCAAAAATCAGGAAATTGGGCTAAAGCAGGTGAAATTTTGGCAGAGCAGGCGCGTTTGCTGGAAAGTATTGGGGCGGAAGGTATTTTAATTGGTGCCAATAc                                                          >  1:863809/1‑211 (MQ=255)

                                                                                                                                                                                                                             gTTTCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATTGTGCAATGCCAAAAATCAGGAAATTGGGCTAAAGCAGGTGAAATTTTGGCAGAGCAGGCGCGTTTGCTGGAAAGTATTGGGGCGGAAGGTATTTTAATTGGTGCCAATAc                                                          >  1:136655/1‑211 (MQ=255)

                                                                                                                                                                                                                               ttCCTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATTGTGCAATGCCAAAAATCAGGAAATTGGGCTAAAGCAGGTGAAATTTTGGCAGAGCAGGCGCGTTTGCTGGAAAGTATTGGGGCGGAAGGTATTTTAATTGGTGCCAATACCATGCACAAAGTCGCGGATGCCGTCCAAAATGTCAttt                    >  1:538229/1‑247 (MQ=255)

                                                                                                                                                                                                                                 ccTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATTGTGCAATGCCAAAAATCAGGAAATTGGGCTAAAGCAGGTGAAATTTTGGCAGAGCAGGCGCGTTTGCTGGAAAGTATTGGGGCGGAAGGTATTTTaa                                                                       >  1:663800/1‑194 (MQ=255)

                                                                                                                                                                                                                                 ccTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATTGTGCAATGCCAAAAATCAGGAAATTGGGCTAAAGCAGGTGAAATTTTGGCAGAGCAGGCGCGTTTGCTGGAAAGTATTGGGGCGGAAGGTATTTTAATTGGTGCCAATACCATGCACAAAGTCGCGGATGCCGTCCAAAATGTCATTTCtgtg               >  1:147765/1‑250 (MQ=255)

                                                                                                                                                                                                                                  cTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAagag                                                                                                                                                                           <  1:529884/93‑1 (MQ=255)

                                                                                                                                                                                                                                  cTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATTGTGCAATGCCAAAAATCAGGAAATTGGGCTAAAGCAGGTGAAATTTTGGCAGAGCAGGCGCGTTTGCTGGAAAGTATTGGGGCGGAAGGTATTTTAATTGGTGCCAATAc                                                          >  1:8294/1‑206 (MQ=255)

                                                                                                                                                                                                                                  cTATTATTTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATTGTGCAATGCCAAAAATCAGGAAATTGGGCTAAAGCAGGTGAAATTTTGGCAGAGCAGGCGCGTTTGCTGGAAAGTATTGGGGCGGAAGGTATTTTAATTGGTGCCAATACCATGCACAAAGTCGCGGATGCCGTCCAAAATGTCATTTCTGTGc              >  1:43087/1‑250 (MQ=255)

                                                                                                                                                                                                                                     ttattTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATTGTGCAATGCCAAAAATCAGGAAATTGGGCTAAAGc                                                                                                                                     <  1:151448/128‑1 (MQ=255)

                                                                                                                                                                                                                                      tattTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATTGTGCAATGCCAAAAATCAGGAAATTGGGCTAAAGCAGGTGAAATTTTGGCAGAGCAGGCGCGTTTGCTGGAAAGTATTgggg                                                                                      <  1:707674/174‑1 (MQ=255)

                                                                                                                                                                                                                                       attTAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATTGTGCAATGCCAAAAATCAGGAAATTGGGCTAAAGCAGGTGAAATTTTGGCAGAGCAGGCGCGTTTGCTGGAAAGTATTGGGGCGGAAGGTATTTTAATTGGTGCCAATACCATGCACAAAGTCGCGGATGCCGTCCAAAATGTCATTTCTGTGCCgttgt        >  1:160570/1‑251 (MQ=255)

                                                                                                                                                                                                                                         ttAATCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATTGTGCAATGCCAAAAATCAGGAAATTGGGCTAAAGCAGGTGAAATTTTGGCAGAGCAGGCGCGTTTGCTGGAAAGTATTGGGGCGGAAGGTATTTTAATTGGTGCCAATACCATGCACAAAGTCGCGGATGCCGTCCAAAATGTCATTTCTGTGCCGTTGTTa      <  1:170892/251‑1 (MQ=255)

                                                                                                                                                                                                                                            aTCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAg                                                                                                                                                                                                                >  1:644809/1‑46 (MQ=255)

                                                                                                                                                                                                                                            aTCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTg                                                                                                                                                                                                   >  1:529954/1‑59 (MQ=255)

                                                                                                                                                                                                                                            aTCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATTGTGCAATGCCAAAAATc                                                                                                                                                       <  1:669438/103‑1 (MQ=255)

                                                                                                                                                                                                                                            aTCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATTGTGCAATGCCAAAAATCAGGAAATTGGGCTAAAGCAGGTGAAATTTTGGCAGAGCAGGCGCGTTTGCTGGAAAGTATTgggg                                                                                      <  1:154763/168‑1 (MQ=255)

                                                                                                                                                                                                                                            aTCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATTGTGCAATGCCAAAAATCAGGAAATTGGGCTAAAGCAGGTGAAATTTTGGCAGAGCAGGCGCGTTTGCTGGAAAGTATTGGGGCGGAAGGTATTTTaa                                                                       >  1:23384/1‑183 (MQ=255)

                                                                                                                                                                                                                                            aTCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATTGTGCAATGCCAAAAATCAGGAAATTGGGCTAAAGCAGGTGAAATTTTGGCAGAGCAGGCGCGTTTGCTGGAAAGTATTGGGGCGGAAGGTATTTTAAt                                                                      >  1:401815/1‑184 (MQ=255)

                                                                                                                                                                                                                                            aTCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATTGTGCAATGCCAAAAATCAGGAAATTGGGCTAAAGCAGGTGAAATTTTGGCAGAGCAGGCGCGTTTGCTGGAAAGTATTGGGGCGGAAGGTATTTTAATTGGTGCCAATAc                                                          >  1:341447/1‑196 (MQ=255)

                                                                                                                                                                                                                                            aTCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATTGTGCAATGCCAAAAATCAGGAAATTGGGCTAAAGCAGGTGAAATTTTGGCAGAGCAGGCGCGTTTGCTGGAAAGTATTGGGGCGGAAGGTATTTTAATTGGTGCCAATAc                                                          >  1:635141/1‑196 (MQ=255)

                                                                                                                                                                                                                                            aTCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATTGTGCAATGCCAAAAATCAGGAAATTGGGCTAAAGCAGGTGAAATTTTGGCAGAGCAGGCGCGTTTGCTGGAAAGTATTGGGGCAGAAGGTATTTTAATTGGTGCCAATAc                                                          >  1:435935/1‑196 (MQ=255)

                                                                                                                                                                                                                                             tCATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATTGTGCAATGCCAAAAATCAGGAAATTGGGCTAAAGCAGGTGAAATTTTGGCAGAGCAGGCGCGTTTGCTGGAAAGTATTGGGGCGGAAGGTATTTTAATTGGTGCCAATACCATGCACAAAGTCGCGGATGCCGTCCAAAATGTCATTTCTGTGCCGTTGTTACata  >  1:775043/1‑251 (MQ=255)

                                                                                                                                                                                                                                              cATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATTGTGCAATGCCAAAAATCAGGAAATTGGGCTAAAGCAGGTGAAATTTTGGCAGAGCAGGCGCGTTTGCTGGAAAGTATTGGGGCGGGAGGTATTTTaa                                                                       >  1:431681/1‑181 (MQ=255)

                                                                                                                                                                                                                                              cATTAACCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATTGTGCAATGCCAAAAATCAGGAAATTGGGCTAAAGCAGGTGAAATTTTGGCAGAGCAGGCGCGTTTGCTGGAAAGTATTGGGGCGGAAGGTATTTTAATTGGTGCCAATAc                                                          <  1:604604/194‑1 (MQ=255)

                                                                                                                                                                                                                                                   aCCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGt                                                                                                                                                                                               <  1:245255/56‑1 (MQ=255)

                                                                                                                                                                                                                                                   aCCGTGCGGTGTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATTGTGCAATGCCAAAAATCAGGAAATTGGGCt                                                                                                                                          >  1:883418/1‑109 (MQ=255)

||

GGGATAATAACAAAATGGCTAAGGAGTTATTTTCTACTTTGAAAAAGCTTTTAATTAAATAATCGTGTAGATGGAACTTATCCTCCCTTGCTGACGCGCGCCGGTATGAATAAAAACGTTTAAAAAAATGACCGCACTTTTATTCGATATGCAAATGGTAAATCAGAGAAAATAACATGAAAACATTAGGTATTTTAGGTGGGATGAGTCCCGAAAGCACGGTTTCCTATTATTTAATCATTAACCGTGCG‑‑GTGAATCAGGCGTTGGGTGGCAATGCAAGTGCGCCGTTATTGATGTCGAGCGTCAATTTTGAAGAGATTGTGCAATGCCAAAAATCAGGAAATTGGGCTAAAGCAGGTGAAATTTTGGCAGAGCAGGCGCGTTTGCTGGAAAGTATTGGGGCGGAAGGTATTTTAATTGGTGCCAATACCATGCACAAAGTCGCGGATGCCGTCCAAAATGTCATTTCTGTGCCGTTGTTACATA  >  1/519453‑519938

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`